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TGFBI cDNA ORF clone, Homo sapiens (human)

Gene Symbol TGFBI
Entrez Gene ID 7045
Full Name transforming growth factor, beta-induced, 68kDa
Synonyms BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5q31

Summary This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601692

Disorder Html: Corneal dystrophy, Groenouw type I, 121900 (3); Corneal dystrophy,

mRNA and Protein(s)

mRNA Protein Name
NM_000358 NP_000349 transforming growth factor-beta-induced protein ig-h3 precursor



Homo sapiens (human) TGFBI NP_000349.1
Pan troglodytes (chimpanzee) TGFBI XP_001169327.1
Macaca mulatta (Rhesus monkey) TGFBI XP_001111447.1
Canis lupus familiaris (dog) TGFBI XP_538640.3
Bos taurus (cattle) TGFBI NP_001192331.1
Mus musculus (house mouse) Tgfbi NP_033395.1
Rattus norvegicus (Norway rat) Tgfbi NP_446254.1
Gallus gallus (chicken) TGFBI NP_990367.1
Danio rerio (zebrafish) tgfbi NP_878282.1
Drosophila melanogaster (fruit fly) mfas NP_788650.1
Xenopus (Silurana) tropicalis (western clawed frog) tgfbi NP_001072329.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TGFBI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGFBI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26539
NM_000358 Homo sapiens transforming growth factor, beta-induced, 68kDa (TGFBI), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26539
Clone ID Related Accession (Same CDS sequence) NM_000358
Accession Version NM_000358.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2052bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product transforming growth factor-beta-induced protein ig-h3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA051240.1, BC000097.2 and M77349.1. This sequence is a reference standard in the RefSeqGene project. On Mar 21, 2008 this sequence version replaced gi:4507466. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000097.2, AK094581.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCGCTCT TCGTGCGGCT GCTGGCTCTC GCCCTGGCTC TGGCCCTGGG CCCCGCCGCG 
ACCCTGGCGG GTCCCGCCAA GTCGCCCTAC CAGCTGGTGC TGCAGCACAG CAGGCTCCGG
GGCCGCCAGC ACGGCCCCAA CGTGTGTGCT GTGCAGAAGG TTATTGGCAC TAATAGGAAG
TACTTCACCA ACTGCAAGCA GTGGTACCAA AGGAAAATCT GTGGCAAATC AACAGTCATC
AGCTACGAGT GCTGTCCTGG ATATGAAAAG GTCCCTGGGG AGAAGGGCTG TCCAGCAGCC
CTACCACTCT CAAACCTTTA CGAGACCCTG GGAGTCGTTG GATCCACCAC CACTCAGCTG
TACACGGACC GCACGGAGAA GCTGAGGCCT GAGATGGAGG GGCCCGGCAG CTTCACCATC
TTCGCCCCTA GCAACGAGGC CTGGGCCTCC TTGCCAGCTG AAGTGCTGGA CTCCCTGGTC
AGCAATGTCA ACATTGAGCT GCTCAATGCC CTCCGCTACC ATATGGTGGG CAGGCGAGTC
CTGACTGATG AGCTGAAACA CGGCATGACC CTCACCTCTA TGTACCAGAA TTCCAACATC
CAGATCCACC ACTATCCTAA TGGGATTGTA ACTGTGAACT GTGCCCGGCT GCTGAAAGCC
GACCACCATG CAACCAACGG GGTGGTGCAC CTCATCGATA AGGTCATCTC CACCATCACC
AACAACATCC AGCAGATCAT TGAGATCGAG GACACCTTTG AGACCCTTCG GGCTGCTGTG
GCTGCATCAG GGCTCAACAC GATGCTTGAA GGTAACGGCC AGTACACGCT TTTGGCCCCG
ACCAATGAGG CCTTCGAGAA GATCCCTAGT GAGACTTTGA ACCGTATCCT GGGCGACCCA
GAAGCCCTGA GAGACCTGCT GAACAACCAC ATCTTGAAGT CAGCTATGTG TGCTGAAGCC
ATCGTTGCGG GGCTGTCTGT AGAGACCCTG GAGGGCACGA CACTGGAGGT GGGCTGCAGC
GGGGACATGC TCACTATCAA CGGGAAGGCG ATCATCTCCA ATAAAGACAT CCTAGCCACC
AACGGGGTGA TCCACTACAT TGATGAGCTA CTCATCCCAG ACTCAGCCAA GACACTATTT
GAATTGGCTG CAGAGTCTGA TGTGTCCACA GCCATTGACC TTTTCAGACA AGCCGGCCTC
GGCAATCATC TCTCTGGAAG TGAGCGGTTG ACCCTCCTGG CTCCCCTGAA TTCTGTATTC
AAAGATGGAA CCCCTCCAAT TGATGCCCAT ACAAGGAATT TGCTTCGGAA CCACATAATT
AAAGACCAGC TGGCCTCTAA GTATCTGTAC CATGGACAGA CCCTGGAAAC TCTGGGCGGC
AAAAAACTGA GAGTTTTTGT TTATCGTAAT AGCCTCTGCA TTGAGAACAG CTGCATCGCG
GCCCACGACA AGAGGGGGAG GTACGGGACC CTGTTCACGA TGGACCGGGT GCTGACCCCC
CCAATGGGGA CTGTCATGGA TGTCCTGAAG GGAGACAATC GCTTTAGCAT GCTGGTAGCT
GCCATCCAGT CTGCAGGACT GACGGAGACC CTCAACCGGG AAGGAGTCTA CACAGTCTTT
GCTCCCACAA ATGAAGCCTT CCGAGCCCTG CCACCAAGAG AACGGAGCAG ACTCTTGGGA
GATGCCAAGG AACTTGCCAA CATCCTGAAA TACCACATTG GTGATGAAAT CCTGGTTAGC
GGAGGCATCG GGGCCCTGGT GCGGCTAAAG TCTCTCCAAG GTGACAAGCT GGAAGTCAGC
TTGAAAAACA ATGTGGTGAG TGTCAACAAG GAGCCTGTTG CCGAGCCTGA CATCATGGCC
ACAAATGGCG TGGTCCATGT CATCACCAAT GTTCTGCAGC CTCCAGCCAA CAGACCTCAG
GAAAGAGGGG ATGAACTTGC AGACTCTGCG CTTGAGATCT TCAAACAAGC ATCAGCGTTT
TCCAGGGCTT CCCAGAGGTC TGTGCGACTA GCCCCTGTCT ATCAAAAGTT ATTAGAGAGG
ATGAAGCATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000349.1
CDS162..2213
Misc Feature(1)132..134(+)
Misc Feature(2)294..437(+)
Misc Feature(3)558..869(+)
Misc Feature(4)918..1280(+)
Misc Feature(5)1317..1661(+)
Misc Feature(6)1701..2063(+)
Misc Feature(7)2085..2093(+)
Exon (1)1..295
Gene:TGFBI
Gene Synonym:
Exon (2)296..394
Gene:TGFBI
Gene Synonym:
Exon (3)395..459
Gene:TGFBI
Gene Synonym:
Exon (4)460..620
Gene:TGFBI
Gene Synonym:
Exon (5)621..785
Gene:TGFBI
Gene Synonym:
Exon (6)786..932
Gene:TGFBI
Gene Synonym:
Exon (7)933..1074
Gene:TGFBI
Gene Synonym:
Exon (8)1075..1287
Gene:TGFBI
Gene Synonym:
Exon (9)1288..1425
Gene:TGFBI
Gene Synonym:
Exon (10)1426..1571
Gene:TGFBI
Gene Synonym:
Exon (11)1572..1708
Gene:TGFBI
Gene Synonym:
Exon (12)1709..1839
Gene:TGFBI
Gene Synonym:
Exon (13)1840..1964
Gene:TGFBI
Gene Synonym:
Exon (14)1965..2067
Gene:TGFBI
Gene Synonym:
Exon (15)2068..2147
Gene:TGFBI
Gene Synonym:
Exon (16)2148..2172
Gene:TGFBI
Gene Synonym:
Exon (17)2173..2805
Gene:TGFBI
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000358
Organism Homo sapiens (human)
Definition Homo sapiens transforming growth factor, beta-induced, 68kDa (TGFBI), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000358

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCGCTCT TCGTGCGGCT GCTGGCTCTC GCCCTGGCTC TGGCCCTGGG CCCCGCCGCG 
ACCCTGGCGG GTCCCGCCAA GTCGCCCTAC CAGCTGGTGC TGCAGCACAG CAGGCTCCGG
GGCCGCCAGC ACGGCCCCAA CGTGTGTGCT GTGCAGAAGG TTATTGGCAC TAATAGGAAG
TACTTCACCA ACTGCAAGCA GTGGTACCAA AGGAAAATCT GTGGCAAATC AACAGTCATC
AGCTACGAGT GCTGTCCTGG ATATGAAAAG GTCCCTGGGG AGAAGGGCTG TCCAGCAGCC
CTACCACTCT CAAACCTTTA CGAGACCCTG GGAGTCGTTG GATCCACCAC CACTCAGCTG
TACACGGACC GCACGGAGAA GCTGAGGCCT GAGATGGAGG GGCCCGGCAG CTTCACCATC
TTCGCCCCTA GCAACGAGGC CTGGGCCTCC TTGCCAGCTG AAGTGCTGGA CTCCCTGGTC
AGCAATGTCA ACATTGAGCT GCTCAATGCC CTCCGCTACC ATATGGTGGG CAGGCGAGTC
CTGACTGATG AGCTGAAACA CGGCATGACC CTCACCTCTA TGTACCAGAA TTCCAACATC
CAGATCCACC ACTATCCTAA TGGGATTGTA ACTGTGAACT GTGCCCGGCT GCTGAAAGCC
GACCACCATG CAACCAACGG GGTGGTGCAC CTCATCGATA AGGTCATCTC CACCATCACC
AACAACATCC AGCAGATCAT TGAGATCGAG GACACCTTTG AGACCCTTCG GGCTGCTGTG
GCTGCATCAG GGCTCAACAC GATGCTTGAA GGTAACGGCC AGTACACGCT TTTGGCCCCG
ACCAATGAGG CCTTCGAGAA GATCCCTAGT GAGACTTTGA ACCGTATCCT GGGCGACCCA
GAAGCCCTGA GAGACCTGCT GAACAACCAC ATCTTGAAGT CAGCTATGTG TGCTGAAGCC
ATCGTTGCGG GGCTGTCTGT AGAGACCCTG GAGGGCACGA CACTGGAGGT GGGCTGCAGC
GGGGACATGC TCACTATCAA CGGGAAGGCG ATCATCTCCA ATAAAGACAT CCTAGCCACC
AACGGGGTGA TCCACTACAT TGATGAGCTA CTCATCCCAG ACTCAGCCAA GACACTATTT
GAATTGGCTG CAGAGTCTGA TGTGTCCACA GCCATTGACC TTTTCAGACA AGCCGGCCTC
GGCAATCATC TCTCTGGAAG TGAGCGGTTG ACCCTCCTGG CTCCCCTGAA TTCTGTATTC
AAAGATGGAA CCCCTCCAAT TGATGCCCAT ACAAGGAATT TGCTTCGGAA CCACATAATT
AAAGACCAGC TGGCCTCTAA GTATCTGTAC CATGGACAGA CCCTGGAAAC TCTGGGCGGC
AAAAAACTGA GAGTTTTTGT TTATCGTAAT AGCCTCTGCA TTGAGAACAG CTGCATCGCG
GCCCACGACA AGAGGGGGAG GTACGGGACC CTGTTCACGA TGGACCGGGT GCTGACCCCC
CCAATGGGGA CTGTCATGGA TGTCCTGAAG GGAGACAATC GCTTTAGCAT GCTGGTAGCT
GCCATCCAGT CTGCAGGACT GACGGAGACC CTCAACCGGG AAGGAGTCTA CACAGTCTTT
GCTCCCACAA ATGAAGCCTT CCGAGCCCTG CCACCAAGAG AACGGAGCAG ACTCTTGGGA
GATGCCAAGG AACTTGCCAA CATCCTGAAA TACCACATTG GTGATGAAAT CCTGGTTAGC
GGAGGCATCG GGGCCCTGGT GCGGCTAAAG TCTCTCCAAG GTGACAAGCT GGAAGTCAGC
TTGAAAAACA ATGTGGTGAG TGTCAACAAG GAGCCTGTTG CCGAGCCTGA CATCATGGCC
ACAAATGGCG TGGTCCATGT CATCACCAAT GTTCTGCAGC CTCCAGCCAA CAGACCTCAG
GAAAGAGGGG ATGAACTTGC AGACTCTGCG CTTGAGATCT TCAAACAAGC ATCAGCGTTT
TCCAGGGCTT CCCAGAGGTC TGTGCGACTA GCCCCTGTCT ATCAAAAGTT ATTAGAGAGG
ATGAAGCATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Development of allele-specific gene-silencing siRNAs for TGFBI Arg124Cys in lattice corneal dystrophy type I
Invest. Ophthalmol. Vis. Sci. 55 (2), 977-985 (2014)
Courtney DG, Atkinson SD, Moore JE, Maurizi E, Serafini C, Pellegrini G, Black GC, Manson FD, Yam GH, Macewen CJ, Allen EH, McLean WH and Moore CB.


book

POSTN/TGFBI-associated stromal signature predicts poor prognosis in serous epithelial ovarian cancer
Gynecol. Oncol. 132 (2), 334-342 (2014)
Karlan BY, Dering J, Walsh C, Orsulic S, Lester J, Anderson LA, Ginther CL, Fejzo M and Slamon D.


book

Mutation in transforming growth factor beta induced protein associated with granular corneal dystrophy type 1 reduces the proteolytic susceptibility through local structural stabilization
Biochim. Biophys. Acta 1834 (12), 2812-2822 (2013)
Underhaug J, Koldso H, Runager K, Nielsen JT, Sorensen CS, Kristensen T, Otzen DE, Karring H, Malmendal A, Schiott B, Enghild JJ and Nielsen NC.


book

Benzalkonium chloride accelerates the formation of the amyloid fibrils of corneal dystrophy-associated peptides
J. Biol. Chem. 288 (35), 25109-25118 (2013)
Kato Y, Yagi H, Kaji Y, Oshika T and Goto Y.


book

Oncostatin M promotes mesenchymal stem cell-stimulated tumor growth through a paracrine mechanism involving periostin and TGFBI
Int. J. Biochem. Cell Biol. 45 (8), 1869-1877 (2013)
Lee MJ, Heo SC, Shin SH, Kwon YW, Do EK, Suh DS, Yoon MS and Kim JH.


book

Beta IG-H3, a novel secretory protein inducible by transforming growth factor-beta, is present in normal skin and promotes the adhesion and spreading of dermal fibroblasts in vitro
J. Invest. Dermatol. 104 (5), 844-849 (1995)
LeBaron RG, Bezverkov KI, Zimber MP, Pavelec R, Skonier J and Purchio AF.


book

cDNA from human ocular ciliary epithelium homologous to beta ig-h3 is preferentially expressed as an extracellular protein in the corneal epithelium
J. Cell. Physiol. 160 (3), 511-521 (1994)
Escribano J, Hernando N, Ghosh S, Crabb J and Coca-Prados M.


book

beta ig-h3: a transforming growth factor-beta-responsive gene encoding a secreted protein that inhibits cell attachment in vitro and suppresses the growth of CHO cells in nude mice
DNA Cell Biol. 13 (6), 571-584 (1994)
Skonier J, Bennett K, Rothwell V, Kosowski S, Plowman G, Wallace P, Edelhoff S, Disteche C, Neubauer M and Marquardt H.


book

Three autosomal dominant corneal dystrophies map to chromosome 5q
Nat. Genet. 6 (1), 47-51 (1994)
Stone EM, Mathers WD, Rosenwasser GO, Holland EJ, Folberg R, Krachmer JH, Nichols BE, Gorevic PD, Taylor CM and Streb LM.


book

cDNA cloning and sequence analysis of beta ig-h3, a novel gene induced in a human adenocarcinoma cell line after treatment with transforming growth factor-beta
DNA Cell Biol. 11 (7), 511-522 (1992)
Skonier J, Neubauer M, Madisen L, Bennett K, Plowman GD and Purchio AF.


 
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