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TLR1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol TLR1
Entrez Gene ID 7096
Full Name toll-like receptor 1
Synonyms CD281, TIL, TIL. LPRS5, rsc786
General protein information
Preferred Names
toll-like receptor 1
Names
toll-like receptor 1
toll/interleukin-1 receptor-like protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4p14

Summary The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601194

Disorder Html: {Leprosy, protection against}, 613223 (3); {Leprosy, susceptibility

mRNA and Protein(s)

mRNA Protein Name
XM_005262662 XP_005262719 toll-like receptor 1 isoform X1
XM_011513742 XP_011512044 toll-like receptor 1 isoform X1
XM_006714028 XP_006714091 toll-like receptor 1 isoform X1
XM_011513743 XP_011512045 toll-like receptor 1 isoform X1
XM_011513744 XP_011512046 toll-like receptor 1 isoform X1
XM_011513745 XP_011512047 toll-like receptor 1 isoform X1
NM_003263 NP_003254 toll-like receptor 1 precursor



Homo sapiens (human) TLR1 NP_003254.2
Pan troglodytes (chimpanzee) TLR1 NP_001123937.1
Macaca mulatta (Rhesus monkey) TLR1 NP_001123896.1
Canis lupus familiaris (dog) TLR1 NP_001139615.1
Mus musculus (house mouse) Tlr1 NP_001263374.1
Rattus norvegicus (Norway rat) Tlr1 NP_001165591.1
Danio rerio (zebrafish) tlr1 NP_001124065.1
Xenopus (Silurana) tropicalis (western clawed frog) tlr1 XP_002938702.2
Xenopus (Silurana) tropicalis (western clawed frog) LOC100495326 XP_002938703.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TLR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19306
XM_005262662 PREDICTED: Homo sapiens toll-like receptor 1 (TLR1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu19306
XM_011513742 PREDICTED: Homo sapiens toll-like receptor 1 (TLR1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu19306
XM_006714028 PREDICTED: Homo sapiens toll-like receptor 1 (TLR1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu19306
XM_011513743 PREDICTED: Homo sapiens toll-like receptor 1 (TLR1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu19306
XM_011513744 PREDICTED: Homo sapiens toll-like receptor 1 (TLR1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu19306
XM_011513745 PREDICTED: Homo sapiens toll-like receptor 1 (TLR1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu19306
NM_003263 Homo sapiens toll-like receptor 1 (TLR1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19306
Clone ID Related Accession (Same CDS sequence) NM_003263 , XM_005262662 , XM_006714028 , XM_011513742 , XM_011513743 , XM_011513744 , XM_011513745
Accession Version XM_005262662.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2361bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product toll-like receptor 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006238.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578808583. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005262719.1
CDS960..3320
Misc Feature(1)1098..1169(+)
Misc Feature(2)1164..1331(+)
Misc Feature(3)1167..1286(+)
Misc Feature(4)1170..1241(+)
Misc Feature(5)1242..1304(+)
Misc Feature(6)1305..1379(+)
Misc Feature(7)1380..1415(+)
Misc Feature(8)2013..2078(+)
Misc Feature(9)2016..2381(+)
Misc Feature(10)2028..2312(+)
Misc Feature(11)2079..2156(+)
Misc Feature(12)2157..2231(+)
Misc Feature(13)2232..2297(+)
Misc Feature(14)2292..2462(+)
Misc Feature(15)2298..2366(+)
Misc Feature(16)2367..2435(+)
Misc Feature(17)2439..2504(+)
Misc Feature(18)2505..2534(+)
Misc Feature(19)2529..2690(+)
Misc Feature(20)2865..3296(+)
Translation

Target ORF information:

RefSeq Version XM_005262662
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens toll-like receptor 1 (TLR1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005262662

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19306
Clone ID Related Accession (Same CDS sequence) NM_003263 , XM_005262662 , XM_006714028 , XM_011513742 , XM_011513743 , XM_011513744 , XM_011513745
Accession Version XM_011513742.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2361bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product toll-like receptor 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006238.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512044.1
CDS883..3243
Misc Feature(1)1021..1092(+)
Misc Feature(2)1087..1254(+)
Misc Feature(3)1090..1209(+)
Misc Feature(4)1093..1164(+)
Misc Feature(5)1165..1227(+)
Misc Feature(6)1228..1302(+)
Misc Feature(7)1303..1338(+)
Misc Feature(8)1936..2001(+)
Misc Feature(9)1939..2304(+)
Misc Feature(10)1951..2235(+)
Misc Feature(11)2002..2079(+)
Misc Feature(12)2080..2154(+)
Misc Feature(13)2155..2220(+)
Misc Feature(14)2215..2385(+)
Misc Feature(15)2221..2289(+)
Misc Feature(16)2290..2358(+)
Misc Feature(17)2362..2427(+)
Misc Feature(18)2428..2457(+)
Misc Feature(19)2452..2613(+)
Misc Feature(20)2788..3219(+)
Translation

Target ORF information:

RefSeq Version XM_011513742
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens toll-like receptor 1 (TLR1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513742

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19306
Clone ID Related Accession (Same CDS sequence) NM_003263 , XM_005262662 , XM_006714028 , XM_011513742 , XM_011513743 , XM_011513744 , XM_011513745
Accession Version XM_006714028.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2361bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product toll-like receptor 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006238.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578808585. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006714091.1
CDS287..2647
Misc Feature(1)425..496(+)
Misc Feature(2)491..658(+)
Misc Feature(3)494..613(+)
Misc Feature(4)497..568(+)
Misc Feature(5)569..631(+)
Misc Feature(6)632..706(+)
Misc Feature(7)707..742(+)
Misc Feature(8)1340..1405(+)
Misc Feature(9)1343..1708(+)
Misc Feature(10)1355..1639(+)
Misc Feature(11)1406..1483(+)
Misc Feature(12)1484..1558(+)
Misc Feature(13)1559..1624(+)
Misc Feature(14)1619..1789(+)
Misc Feature(15)1625..1693(+)
Misc Feature(16)1694..1762(+)
Misc Feature(17)1766..1831(+)
Misc Feature(18)1832..1861(+)
Misc Feature(19)1856..2017(+)
Misc Feature(20)2192..2623(+)
Translation

Target ORF information:

RefSeq Version XM_006714028
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens toll-like receptor 1 (TLR1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006714028

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19306
Clone ID Related Accession (Same CDS sequence) NM_003263 , XM_005262662 , XM_006714028 , XM_011513742 , XM_011513743 , XM_011513744 , XM_011513745
Accession Version XM_011513743.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2361bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product toll-like receptor 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006238.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512045.1
CDS905..3265
Misc Feature(1)1043..1114(+)
Misc Feature(2)1109..1276(+)
Misc Feature(3)1112..1231(+)
Misc Feature(4)1115..1186(+)
Misc Feature(5)1187..1249(+)
Misc Feature(6)1250..1324(+)
Misc Feature(7)1325..1360(+)
Misc Feature(8)1958..2023(+)
Misc Feature(9)1961..2326(+)
Misc Feature(10)1973..2257(+)
Misc Feature(11)2024..2101(+)
Misc Feature(12)2102..2176(+)
Misc Feature(13)2177..2242(+)
Misc Feature(14)2237..2407(+)
Misc Feature(15)2243..2311(+)
Misc Feature(16)2312..2380(+)
Misc Feature(17)2384..2449(+)
Misc Feature(18)2450..2479(+)
Misc Feature(19)2474..2635(+)
Misc Feature(20)2810..3241(+)
Translation

Target ORF information:

RefSeq Version XM_011513743
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens toll-like receptor 1 (TLR1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513743

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19306
Clone ID Related Accession (Same CDS sequence) NM_003263 , XM_005262662 , XM_006714028 , XM_011513742 , XM_011513743 , XM_011513744 , XM_011513745
Accession Version XM_011513744.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2361bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product toll-like receptor 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006238.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512046.1
CDS828..3188
Misc Feature(1)966..1037(+)
Misc Feature(2)1032..1199(+)
Misc Feature(3)1035..1154(+)
Misc Feature(4)1038..1109(+)
Misc Feature(5)1110..1172(+)
Misc Feature(6)1173..1247(+)
Misc Feature(7)1248..1283(+)
Misc Feature(8)1881..1946(+)
Misc Feature(9)1884..2249(+)
Misc Feature(10)1896..2180(+)
Misc Feature(11)1947..2024(+)
Misc Feature(12)2025..2099(+)
Misc Feature(13)2100..2165(+)
Misc Feature(14)2160..2330(+)
Misc Feature(15)2166..2234(+)
Misc Feature(16)2235..2303(+)
Misc Feature(17)2307..2372(+)
Misc Feature(18)2373..2402(+)
Misc Feature(19)2397..2558(+)
Misc Feature(20)2733..3164(+)
Translation

Target ORF information:

RefSeq Version XM_011513744
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens toll-like receptor 1 (TLR1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513744

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19306
Clone ID Related Accession (Same CDS sequence) NM_003263 , XM_005262662 , XM_006714028 , XM_011513742 , XM_011513743 , XM_011513744 , XM_011513745
Accession Version XM_011513745.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2361bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product toll-like receptor 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006238.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512047.1
CDS791..3151
Misc Feature(1)929..1000(+)
Misc Feature(2)995..1162(+)
Misc Feature(3)998..1117(+)
Misc Feature(4)1001..1072(+)
Misc Feature(5)1073..1135(+)
Misc Feature(6)1136..1210(+)
Misc Feature(7)1211..1246(+)
Misc Feature(8)1844..1909(+)
Misc Feature(9)1847..2212(+)
Misc Feature(10)1859..2143(+)
Misc Feature(11)1910..1987(+)
Misc Feature(12)1988..2062(+)
Misc Feature(13)2063..2128(+)
Misc Feature(14)2123..2293(+)
Misc Feature(15)2129..2197(+)
Misc Feature(16)2198..2266(+)
Misc Feature(17)2270..2335(+)
Misc Feature(18)2336..2365(+)
Misc Feature(19)2360..2521(+)
Misc Feature(20)2696..3127(+)
Translation

Target ORF information:

RefSeq Version XM_011513745
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens toll-like receptor 1 (TLR1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513745

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19306
Clone ID Related Accession (Same CDS sequence) NM_003263 , XM_005262662 , XM_006714028 , XM_011513742 , XM_011513743 , XM_011513744 , XM_011513745
Accession Version NM_003263.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2361bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product toll-like receptor 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL050262.1, AW408740.1, D13637.1 and BU623316.1. This sequence is a reference standard in the RefSeqGene project. On or before Sep 20, 2006 this sequence version replaced gi:113431160, gi:19718732. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL050262.1, AK292982.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003254.2
CDS275..2635
Misc Feature(1)263..265(+)
Misc Feature(2)413..484(+)
Misc Feature(3)434..505(+)
Misc Feature(4)479..646(+)
Misc Feature(5)482..601(+)
Misc Feature(6)485..556(+)
Misc Feature(7)506..577(+)
Misc Feature(8)557..619(+)
Misc Feature(9)578..649(+)
Misc Feature(10)620..694(+)
Misc Feature(11)650..724(+)
Misc Feature(12)695..730(+)
Misc Feature(13)725..799(+)
Misc Feature(14)800..871(+)
Misc Feature(15)872..943(+)
Misc Feature(16)944..1024(+)
Misc Feature(17)1025..1108(+)
Misc Feature(18)1109..1198(+)
Misc Feature(19)1199..1285(+)
Misc Feature(20)1211..1222(+)
Misc Feature(21)1286..1357(+)
Misc Feature(22)1328..1393(+)
Misc Feature(23)1331..1696(+)
Misc Feature(24)1343..1627(+)
Misc Feature(25)1358..1438(+)
Misc Feature(26)1394..1471(+)
Misc Feature(27)1439..1516(+)
Misc Feature(28)1472..1546(+)
Misc Feature(29)1517..1585(+)
Misc Feature(30)1547..1612(+)
Misc Feature(31)1586..1645(+)
Misc Feature(32)1607..1777(+)
Misc Feature(33)1613..1681(+)
Misc Feature(34)1646..1708(+)
Misc Feature(35)1682..1750(+)
Misc Feature(36)1709..1774(+)
Misc Feature(37)1754..1819(+)
Misc Feature(38)1775..1846(+)
Misc Feature(39)1820..1849(+)
Misc Feature(40)1844..2005(+)
Misc Feature(41)2015..2077(+)
Misc Feature(42)2180..2611(+)
Exon (1)1..38
Gene:TLR1
Gene Synonym:
Exon (2)39..115
Gene:TLR1
Gene Synonym:
Exon (3)116..207
Gene:TLR1
Gene Synonym:
Exon (4)208..2851
Gene:TLR1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003263
Organism Homo sapiens (human)
Definition Homo sapiens toll-like receptor 1 (TLR1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003263

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGACTAGCA TCTTCCATTT TGCCATTATC TTCATGTTAA TACTTCAGAT CAGAATACAA 
TTATCTGAAG AAAGTGAATT TTTAGTTGAT AGGTCAAAAA ACGGTCTCAT CCACGTTCCT
AAAGACCTAT CCCAGAAAAC AACAATCTTA AATATATCGC AAAATTATAT ATCTGAGCTT
TGGACTTCTG ACATCTTATC ACTGTCAAAA CTGAGGATTT TGATAATTTC TCATAATAGA
ATCCAGTATC TTGATATCAG TGTTTTCAAA TTCAACCAGG AATTGGAATA CTTGGATTTG
TCCCACAACA AGTTGGTGAA GATTTCTTGC CACCCTACTG TGAACCTCAA GCACTTGGAC
CTGTCATTTA ATGCATTTGA TGCCCTGCCT ATATGCAAAG AGTTTGGCAA TATGTCTCAA
CTAAAATTTC TGGGGTTGAG CACCACACAC TTAGAAAAAT CTAGTGTGCT GCCAATTGCT
CATTTGAATA TCAGCAAGGT CTTGCTGGTC TTAGGAGAGA CTTATGGGGA AAAAGAAGAC
CCTGAGGGCC TTCAAGACTT TAACACTGAG AGTCTGCACA TTGTGTTCCC CACAAACAAA
GAATTCCATT TTATTTTGGA TGTGTCAGTC AAGACTGTAG CAAATCTGGA ACTATCTAAT
ATCAAATGTG TGCTAGAAGA TAACAAATGT TCTTACTTCC TAAGTATTCT GGCGAAACTT
CAAACAAATC CAAAGTTATC AAATCTTACC TTAAACAACA TTGAAACAAC TTGGAATTCT
TTCATTAGGA TCCTCCAGCT GGTTTGGCAT ACAACTGTAT GGTATTTCTC AATTTCAAAC
GTGAAGCTAC AGGGTCAGCT GGACTTCAGA GATTTTGATT ATTCTGGCAC TTCCTTGAAG
GCCTTGTCTA TACACCAAGT TGTCAGCGAT GTGTTCGGTT TTCCGCAAAG TTATATCTAT
GAAATCTTTT CGAATATGAA CATCAAAAAT TTCACAGTGT CTGGTACACG CATGGTCCAC
ATGCTTTGCC CATCCAAAAT TAGCCCGTTC CTGCATTTGG ATTTTTCCAA TAATCTCTTA
ACAGACACGG TTTTTGAAAA TTGTGGGCAC CTTACTGAGT TGGAGACACT TATTTTACAA
ATGAATCAAT TAAAAGAACT TTCAAAAATA GCTGAAATGA CTACACAGAT GAAGTCTCTG
CAACAATTGG ATATTAGCCA GAATTCTGTA AGCTATGATG AAAAGAAAGG AGACTGTTCT
TGGACTAAAA GTTTATTAAG TTTAAATATG TCTTCAAATA TACTTACTGA CACTATTTTC
AGATGTTTAC CTCCCAGGAT CAAGGTACTT GATCTTCACA GCAATAAAAT AAAGAGCATT
CCTAAACAAG TCGTAAAACT GGAAGCTTTG CAAGAACTCA ATGTTGCTTT CAATTCTTTA
ACTGACCTTC CTGGATGTGG CAGCTTTAGC AGCCTTTCTG TATTGATCAT TGATCACAAT
TCAGTTTCCC ACCCATCGGC TGATTTCTTC CAGAGCTGCC AGAAGATGAG GTCAATAAAA
GCAGGGGACA ATCCATTCCA ATGTACCTGT GAGCTAGGAG AATTTGTCAA AAATATAGAC
CAAGTATCAA GTGAAGTGTT AGAGGGCTGG CCTGATTCTT ATAAGTGTGA CTACCCGGAA
AGTTATAGAG GAACCCTACT AAAGGACTTT CACATGTCTG AATTATCCTG CAACATAACT
CTGCTGATCG TCACCATCGT TGCCACCATG CTGGTGTTGG CTGTGACTGT GACCTCCCTC
TGCAGCTACT TGGATCTGCC CTGGTATCTC AGGATGGTGT GCCAGTGGAC CCAGACCCGG
CGCAGGGCCA GGAACATACC CTTAGAAGAA CTCCAAAGAA ATCTCCAGTT TCATGCATTT
ATTTCATATA GTGGGCACGA TTCTTTCTGG GTGAAGAATG AATTATTGCC AAACCTAGAG
AAAGAAGGTA TGCAGATTTG CCTTCATGAG AGAAACTTTG TTCCTGGCAA GAGCATTGTG
GAAAATATCA TCACCTGCAT TGAGAAGAGT TACAAGTCCA TCTTTGTTTT GTCTCCCAAC
TTTGTCCAGA GTGAATGGTG CCATTATGAA CTCTACTTTG CCCATCACAA TCTCTTTCAT
GAAGGATCTA ATAGCTTAAT CCTGATCTTG CTGGAACCCA TTCCGCAGTA CTCCATTCCT
AGCAGTTATC ACAAGCTCAA AAGTCTCATG GCCAGGAGGA CTTATTTGGA ATGGCCCAAG
GAAAAGAGCA AACGTGGCCT TTTTTGGGCT AACTTAAGGG CAGCCATTAA TATTAAGCTG
ACAGAGCAAG CAAAGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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