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ACTG1 actin gamma 1 [Homo sapiens (human)]

Gene Symbol ACTG1
Entrez Gene ID 71
Full Name actin gamma 1
Synonyms ACT, ACTG, BRWS2, DFNA20, DFNA26, HEL-176
General protein information
Preferred Names
actin, cytoplasmic 2
Names
actin, cytoplasmic 2
cytoskeletal gamma-actin
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q25

Summary Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]. lac of sum
Disorder MIM:

102560

Disorder Html: Deafness, autosomal dominant 20/26, 604717 (3)





The following ACTG1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTG1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu14728 NM_001614 Homo sapiens actin gamma 1 (ACTG1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $379
OHu14728 NM_001199954 Homo sapiens actin gamma 1 (ACTG1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu14728
Accession Version NM_001614.3
Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product actin, cytoplasmic 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455954.1, X04098.1 and BC063495.1. This sequence is a reference standard in the RefSeqGene project. On Jan 4, 2011 this sequence version replaced gi:11038618. Summary: Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007442.2, BC000292.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001605.1
CDS140..1267
Misc Feature(1)38..40(+)
Misc Feature(2)140..142(+)
Misc Feature(3)143..145(+)
Misc Feature(4)146..1264(+)
Misc Feature(5)161..682(+)
Misc Feature(6)170..610(+)
Misc Feature(7)269..271(+)
Misc Feature(8)278..280(+)
Misc Feature(9)356..358(+)
Misc Feature(10)389..391(+)
Misc Feature(11)788..790(+)
Exon (1)1..133
Gene:ACTG1
Gene Synonym:
Exon (2)134..262
Gene:ACTG1
Gene Synonym:
Exon (3)263..502
Gene:ACTG1
Gene Synonym:
Exon (4)503..941
Gene:ACTG1
Gene Synonym:
Exon (5)942..1123
Gene:ACTG1
Gene Synonym:
Exon (6)1124..1986
Gene:ACTG1
Gene Synonym:
Translation
Position Chain Variation Link
85 85 a, g dbSNP:7503278
316 316 a, g dbSNP:11549220
405 405 c, t dbSNP:28999111
472 472 c, t dbSNP:727502884
492 492 a, g, t dbSNP:104894544
493 493 a, c, g dbSNP:267606630
498 498 c, t dbSNP:281875325
503 503 a, g dbSNP:281875330
506 506 a, t dbSNP:767493603
538 538 c, t dbSNP:2230158
543 543 c, t dbSNP:11549190
553 553 a, c, g, t dbSNP:143978597
565 565 c, t dbSNP:112043655
571 571 c, g, t dbSNP:372748659
597 597 g, t dbSNP:140176659
603 603 c, t dbSNP:281875326
637 637 c, t dbSNP:373572370
649 649 c, t dbSNP:558086995
659 659 g, t dbSNP:587780274
660 660 c, g dbSNP:727504862
737 737 a, t dbSNP:587780275
747 747 a, c dbSNP:281875327
750 750 c, t dbSNP:11549225
775 775 a, c dbSNP:727502883
835 835 a, c, g, t dbSNP:202155328
860 860 a, g dbSNP:267606631
868 868 c, t dbSNP:2230159
899 899 a, c, t dbSNP:281875328
905 905 a, c, t dbSNP:281875329
930 930 c, t dbSNP:104894546
967 967 a, g dbSNP:727504806
972 972 c, t dbSNP:28999112
992 992 c, t dbSNP:727502882
1057 1057 c, t dbSNP:1139405
1069 1069 c, t dbSNP:1135989
1084 1084 a, g dbSNP:727502881
1133 1133 c, g dbSNP:104894545
1141 1141 a, g dbSNP:113262912
1153 1153 a, g dbSNP:1139807
1165 1165 c, t dbSNP:1139406
1190 1190 a, c dbSNP:727502880
1247 1247 a, g dbSNP:201509098
1248 1248 c, t dbSNP:104894547
1249 1249 a, c dbSNP:727502879
1267 1267 a, g dbSNP:11549223
1326 1326 c, t dbSNP:751477808
1332 1332 c, g, t dbSNP:766222446
1401 1401 c, g, t dbSNP:28478053
1408 1408 c, t dbSNP:55978907
1459 1459 c, g dbSNP:55902924
1485 1485 c, g dbSNP:761318967
1541 1541 c, t dbSNP:776240569
1551 1551 a, g dbSNP:11549167
1627 1627 a, g dbSNP:1140892
1636 1636 a, c, t dbSNP:757530454
1637 1637 a, g dbSNP:1139408
1774 1774 a, g dbSNP:751563267
1792 1792 c, t dbSNP:1139409
1793 1793 c, t dbSNP:1139410
1827 1827 a, g dbSNP:764904808
1867 1867 a, g dbSNP:7502276
1884 1884 a, g dbSNP:1139414

Target ORF information:

RefSeq Version NM_001614
Organism Homo sapiens (human)
Definition Homo sapiens actin gamma 1 (ACTG1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001614

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14728
Accession Version NM_001199954.1
Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product actin, cytoplasmic 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455954.1, AC139149.6 and BC063495.1. Summary: Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC007442.2, BC000292.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001186883.1
CDS259..1386
Misc Feature(1)259..261(+)
Misc Feature(2)262..264(+)
Misc Feature(3)265..1383(+)
Misc Feature(4)280..801(+)
Misc Feature(5)289..729(+)
Misc Feature(6)388..390(+)
Misc Feature(7)397..399(+)
Misc Feature(8)475..477(+)
Misc Feature(9)508..510(+)
Exon (1)1..252
Gene:ACTG1
Gene Synonym:
Exon (2)253..381
Gene:ACTG1
Gene Synonym:
Exon (3)382..621
Gene:ACTG1
Gene Synonym:
Exon (4)622..1060
Gene:ACTG1
Gene Synonym:
Exon (5)1061..1242
Gene:ACTG1
Gene Synonym:
Exon (6)1243..2105
Gene:ACTG1
Gene Synonym:
Translation
Position Chain Variation Link
85 85 a, g dbSNP:7503278
435 435 a, g dbSNP:11549220
524 524 c, t dbSNP:28999111
591 591 c, t dbSNP:727502884
611 611 a, g, t dbSNP:104894544
612 612 a, c, g dbSNP:267606630
617 617 c, t dbSNP:281875325
622 622 a, g dbSNP:281875330
625 625 a, t dbSNP:767493603
657 657 c, t dbSNP:2230158
662 662 c, t dbSNP:11549190
672 672 a, c, g, t dbSNP:143978597
684 684 c, t dbSNP:112043655
690 690 c, g, t dbSNP:372748659
716 716 g, t dbSNP:140176659
722 722 c, t dbSNP:281875326
756 756 c, t dbSNP:373572370
768 768 c, t dbSNP:558086995
778 778 g, t dbSNP:587780274
779 779 c, g dbSNP:727504862
856 856 a, t dbSNP:587780275
866 866 a, c dbSNP:281875327
869 869 c, t dbSNP:11549225
894 894 a, c dbSNP:727502883
954 954 a, c, g, t dbSNP:202155328
979 979 a, g dbSNP:267606631
987 987 c, t dbSNP:2230159
1018 1018 a, c, t dbSNP:281875328
1024 1024 a, c, t dbSNP:281875329
1049 1049 c, t dbSNP:104894546
1086 1086 a, g dbSNP:727504806
1091 1091 c, t dbSNP:28999112
1111 1111 c, t dbSNP:727502882
1176 1176 c, t dbSNP:1139405
1188 1188 c, t dbSNP:1135989
1203 1203 a, g dbSNP:727502881
1252 1252 c, g dbSNP:104894545
1260 1260 a, g dbSNP:113262912
1272 1272 a, g dbSNP:1139807
1284 1284 c, t dbSNP:1139406
1309 1309 a, c dbSNP:727502880
1366 1366 a, g dbSNP:201509098
1367 1367 c, t dbSNP:104894547
1368 1368 a, c dbSNP:727502879
1386 1386 a, g dbSNP:11549223
1445 1445 c, t dbSNP:751477808
1451 1451 c, g, t dbSNP:766222446
1520 1520 c, g, t dbSNP:28478053
1527 1527 c, t dbSNP:55978907
1578 1578 c, g dbSNP:55902924
1604 1604 c, g dbSNP:761318967
1660 1660 c, t dbSNP:776240569
1670 1670 a, g dbSNP:11549167
1746 1746 a, g dbSNP:1140892
1755 1755 a, c, t dbSNP:757530454
1756 1756 a, g dbSNP:1139408
1893 1893 a, g dbSNP:751563267
1911 1911 c, t dbSNP:1139409
1912 1912 c, t dbSNP:1139410
1946 1946 a, g dbSNP:764904808
1986 1986 a, g dbSNP:7502276
2003 2003 a, g dbSNP:1139414

Target ORF information:

RefSeq Version NM_001199954
Organism Homo sapiens (human)
Definition Homo sapiens actin gamma 1 (ACTG1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001199954

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.