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ACTG1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol ACTG1
Entrez Gene ID 71
Full Name actin gamma 1
Synonyms ACT, ACTG, BRWS2, DFNA20, DFNA26, HEL-176
General protein information
Preferred Names
actin, cytoplasmic 2
Names
actin, cytoplasmic 2
cytoskeletal gamma-actin
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q25

Summary Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]. lac of sum
Disorder MIM:

102560

Disorder Html: Deafness, autosomal dominant 20/26, 604717 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001614 NP_001605 actin, cytoplasmic 2
NM_001199954 NP_001186883 actin, cytoplasmic 2



Homo sapiens (human) ACTG1 NP_001605.1
Macaca mulatta (Rhesus monkey) ACTG1 XP_001110892.2
Bos taurus (cattle) ACTG1 NP_001028790.1
Mus musculus (house mouse) Actg1 NP_033739.1
Rattus norvegicus (Norway rat) Actg1 NP_001120921.1
Gallus gallus (chicken) ACTG1 NP_001007825.1
Danio rerio (zebrafish) actb1 NP_571106.1
Danio rerio (zebrafish) actb2 NP_853632.3
Drosophila melanogaster (fruit fly) Act5C NP_001014726.1
Caenorhabditis elegans act-3 NP_505817.1
Caenorhabditis elegans act-1 NP_505819.1
Caenorhabditis elegans act-2 NP_505818.1
Caenorhabditis elegans act-4 NP_508841.1
Arabidopsis thaliana (thale cress) ACT8 NP_175350.1
Arabidopsis thaliana (thale cress) ACT2 NP_188508.1
Xenopus (Silurana) tropicalis (western clawed frog) actg1 NP_989332.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACTG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu14728
NM_001614 Homo sapiens actin gamma 1 (ACTG1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $159.50
$319.00
OHu14728
NM_001199954 Homo sapiens actin gamma 1 (ACTG1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $159.50
$319.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu14728
Clone ID Related Accession (Same CDS sequence) NM_001199954 , NM_001614
Accession Version NM_001614.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product actin, cytoplasmic 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455954.1, X04098.1 and BC063495.1. This sequence is a reference standard in the RefSeqGene project. On Jan 4, 2011 this sequence version replaced gi:11038618. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007442.2, BC000292.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001605.1
CDS140..1267
Misc Feature(1)38..40(+)
Misc Feature(2)140..142(+)
Misc Feature(3)143..145(+)
Misc Feature(4)146..1264(+)
Misc Feature(5)161..682(+)
Misc Feature(6)170..610(+)
Misc Feature(7)269..271(+)
Misc Feature(8)278..280(+)
Misc Feature(9)356..358(+)
Misc Feature(10)389..391(+)
Misc Feature(11)788..790(+)
Exon (1)1..133
Gene:ACTG1
Gene Synonym:
Exon (2)134..262
Gene:ACTG1
Gene Synonym:
Exon (3)263..502
Gene:ACTG1
Gene Synonym:
Exon (4)503..941
Gene:ACTG1
Gene Synonym:
Exon (5)942..1123
Gene:ACTG1
Gene Synonym:
Exon (6)1124..1986
Gene:ACTG1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001614
Organism Homo sapiens (human)
Definition Homo sapiens actin gamma 1 (ACTG1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001614

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14728
Clone ID Related Accession (Same CDS sequence) NM_001199954 , NM_001614
Accession Version NM_001199954.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product actin, cytoplasmic 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455954.1, AC139149.6 and BC063495.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC007442.2, BC000292.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001186883.1
CDS259..1386
Misc Feature(1)259..261(+)
Misc Feature(2)262..264(+)
Misc Feature(3)265..1383(+)
Misc Feature(4)280..801(+)
Misc Feature(5)289..729(+)
Misc Feature(6)388..390(+)
Misc Feature(7)397..399(+)
Misc Feature(8)475..477(+)
Misc Feature(9)508..510(+)
Exon (1)1..252
Gene:ACTG1
Gene Synonym:
Exon (2)253..381
Gene:ACTG1
Gene Synonym:
Exon (3)382..621
Gene:ACTG1
Gene Synonym:
Exon (4)622..1060
Gene:ACTG1
Gene Synonym:
Exon (5)1061..1242
Gene:ACTG1
Gene Synonym:
Exon (6)1243..2105
Gene:ACTG1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001199954
Organism Homo sapiens (human)
Definition Homo sapiens actin gamma 1 (ACTG1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001199954

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The actin/MKL1 signalling pathway influences cell growth and gene expression through large-scale chromatin reorganization and histone post-translational modifications
Biochem. J. 461 (2), 257-268 (2014)
Flouriot G, Huet G, Demay F, Pakdel F, Boujrad N and Michel D.


book

Loss of ASAP3 destabilizes cytoskeletal protein ACTG1 to suppress cancer cell migration
Mol Med Rep 9 (2), 387-394 (2014)
Luo Y, Kong F, Wang Z, Chen D, Liu Q, Wang T, Xu R, Wang X and Yang JY.


book

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma naive to therapy
Mol Biosyst 9 (6), 1108-1116 (2013)
Pieragostino D, Agnifili L, Fasanella V, D'Aguanno S, Mastropasqua R, Di Ilio C, Sacchetta P, Urbani A and Del Boccio P.


book

Platelet proteome analysis reveals integrin-dependent aggregation defects in patients with myelodysplastic syndromes
Mol. Cell Proteomics 12 (5), 1272-1280 (2013)
Frobel J, Cadeddu RP, Hartwig S, Bruns I, Wilk CM, Kundgen A, Fischer JC, Schroeder T, Steidl UG, Germing U, Lehr S, Haas R and Czibere A.


book

Multiphasic analysis of whole exome sequencing data identifies a novel mutation of ACTG1 in a nonsyndromic hearing loss family
BMC Genomics 14, 191 (2013)
Park G, Gim J, Kim AR, Han KH, Kim HS, Oh SH, Park T, Park WY and Choi BY.


book

Deafness and Hereditary Hearing Loss Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Smith,R.J.H., Shearer,A.E., Hildebrand,M.S. and Van Camp,G.


book

Ca2+ and pH determine the interaction of chromaffin cell scinderin with phosphatidylserine and phosphatidylinositol 4,5,-biphosphate and its cellular distribution during nicotinic-receptor stimulation and protein kinase C activation
J. Cell Biol. 119 (4), 797-810 (1992)
Rodriguez Del Castillo A, Vitale ML and Trifaro JM.


book

Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin
J. Infect. 24 (3), 317-320 (1992)
Dawson SJ and White LA.


book

Structure, chromosome location, and expression of the human gamma-actin gene: differential evolution, location, and expression of the cytoskeletal beta- and gamma-actin genes
Mol. Cell. Biol. 8 (4), 1775-1789 (1988)
Erba HP, Eddy R, Shows T, Kedes L and Gunning P.


book

Botulinum C2 toxin ADP-ribosylates cytoplasmic beta/gamma-actin in arginine 177
J. Biol. Chem. 263 (2), 696-700 (1988)
Vandekerckhove J, Schering B, Barmann M and Aktories K.


 
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