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TNF tumor necrosis factor [Homo sapiens (human)]

Gene Symbol TNF
Entrez Gene ID 7124
Full Name tumor necrosis factor
Synonyms DIF, TNF-alpha, TNFA, TNFSF2
General protein information
Preferred Names
tumor necrosis factor
Names
tumor necrosis factor
TNF-a
cachectin
APC1 protein
TNF, monocyte-derived
TNF, macrophage-derived
tumor necrosis factor-alpha
tumor necrosis factor ligand superfamily member 2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.3

Summary This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

191160

Disorder Html: {Malaria, cerebral, susceptibility to}, 611162 (3); {Septic shock,





The following TNF gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27508 NM_000594 Homo sapiens tumor necrosis factor (TNF), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $269

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu27508
Accession Version NM_000594.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP215875.1, BC028148.1 and CA306559.1. This sequence is a reference standard in the RefSeqGene project. On Jul 12, 2012 this sequence version replaced gi:25952110. Summary: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP215875.1, BC028148.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGGCGCT CCCCAAGAAG 
ACAGGGGGGC CCCAGGGCTC CAGGCGGTGC TTGTTCCTCA GCCTCTTCTC CTTCCTGATC
GTGGCAGGCG CCACCACGCT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG
GAAGAGTTCC CCAGGGACCT CTCTCTAATC AGCCCTCTGG CCCAGGCAGT CAGATCATCT
TCTCGAACCC CGAGTGACAA GCCTGTAGCC CATGTTGTAG CAAACCCTCA AGCTGAGGGG
CAGCTCCAGT GGCTGAACCG CCGGGCCAAT GCCCTCCTGG CCAATGGCGT GGAGCTGAGA
GATAACCAGC TGGTGGTGCC ATCAGAGGGC CTGTACCTCA TCTACTCCCA GGTCCTCTTC
AAGGGCCAAG GCTGCCCCTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCATCGCC
GTCTCCTACC AGACCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCCTG CCAGAGGGAG
ACCCCAGAGG GGGCTGAGGC CAAGCCCTGG TATGAGCCCA TCTATCTGGG AGGGGTCTTC
CAGCTGGAGA AGGGTGACCG ACTCAGCGCT GAGATCAATC GGCCCGACTA TCTCGACTTT
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000585.2
CDS176..877
Misc Feature(1)98..100(+)
Misc Feature(2)179..181(+)
Misc Feature(3)230..232(+)
Misc Feature(4)233..235(+)
Misc Feature(5)278..283(+)
Misc Feature(6)281..343(+)
Misc Feature(7)290..295(+)
Misc Feature(8)320..325(+)
Misc Feature(9)326..331(+)
Misc Feature(10)395..397(+)
Misc Feature(11)395..397(+)
Misc Feature(12)395..397(+)
Misc Feature(13)401..406(+)
Misc Feature(14)401..403(+)
Misc Feature(15)401..403(+)
Misc Feature(16)410..412(+)
Misc Feature(17)437..868(+)
Misc Feature(18)446..868(+)
Misc Feature(19)488..670(+)
Exon (1)1..361
Gene:TNF
Gene Synonym:
Exon (2)362..407
Gene:TNF
Gene Synonym:
Exon (3)408..455
Gene:TNF
Gene Synonym:
Exon (4)456..1676
Gene:TNF
Gene Synonym:
Translation
Position Chain Variation Link
5 5 c, t dbSNP:4248164
31 31 a, g dbSNP:61761335
39 39 -, ag dbSNP:4647199
61 61 -, c dbSNP:201328097
63 63 c, g dbSNP:3093660
68 68 -, c dbSNP:4645838
68 68 -, c dbSNP:386594198
116 116 a, g dbSNP:538552423
133 133 a, t dbSNP:372568141
143 143 c, t dbSNP:752785007
144 144 a, g dbSNP:554060079
146 146 c, t dbSNP:372719825
148 148 c, t dbSNP:2515924
149 149 a, c dbSNP:750426362
152 152 a, c dbSNP:755847921
160 160 c, t dbSNP:779966486
170 170 a, g dbSNP:375864945
177 177 c, g, t dbSNP:369780852
188 188 a, c dbSNP:747694851
189 189 a, g dbSNP:771650207
190 190 c, t dbSNP:565862537
197 197 c, t dbSNP:374531985
198 198 a, g dbSNP:201502336
202 202 c, t dbSNP:377627218
203 203 a, g dbSNP:759696973
205 205 a, g dbSNP:770023902
215 215 a, g dbSNP:775506502
222 222 c, g, t dbSNP:763000109
223 223 a, g dbSNP:146375573
231 231 a, g dbSNP:377338702
238 238 a, c dbSNP:766034823
239 239 a, g dbSNP:753713562
245 245 c, g dbSNP:754639425
248 248 c, g dbSNP:764955335
259 259 a, g dbSNP:779870893
261 261 a, g dbSNP:576621666
262 262 g, t dbSNP:2228088
270 270 c, t dbSNP:777405140
272 272 c, t dbSNP:747406665
287 287 c, t dbSNP:757794184
295 295 a, c, t dbSNP:141667614
296 296 a, g, t dbSNP:558678940
304 304 c, t dbSNP:369163834
312 312 c, t dbSNP:200241887
313 313 a, g dbSNP:144480538
316 316 c, t dbSNP:541347219
329 329 a, c dbSNP:3179060
336 336 a, g dbSNP:756182468
344 344 a, g dbSNP:777874746
346 346 c, t dbSNP:776753689
358 358 a, g dbSNP:759378982
360 360 a, g dbSNP:765178016
366 366 c, t dbSNP:35131721
367 367 c, t dbSNP:772331441
371 371 a, g dbSNP:776692566
380 380 c, t dbSNP:759609722
400 400 a, g dbSNP:769643681
407 407 a, g dbSNP:775365890
413 413 c, t dbSNP:369783162
420 420 a, g dbSNP:772270241
426 426 c, t dbSNP:4645843
427 427 a, g dbSNP:745738344
435 435 a, c dbSNP:190788828
455 455 a, g dbSNP:1800620
467 467 a, g dbSNP:375472217
481 481 c, t dbSNP:1800618
495 495 a, g dbSNP:758704433
497 497 c, t dbSNP:281865419
498 498 a, g dbSNP:747115001
502 502 a, c dbSNP:771174703
516 516 a, c dbSNP:780017660
520 520 c, t dbSNP:142488339
524 524 a, g dbSNP:548532642
525 525 c, t dbSNP:774032100
529 529 a, g dbSNP:747751043
534 534 a, g dbSNP:778657377
535 535 a, g dbSNP:569852154
556 556 a, g dbSNP:751421650
561 561 a, g dbSNP:772893367
562 562 a, g dbSNP:537268309
565 565 c, t dbSNP:150508976
574 574 c, t dbSNP:777187968
587 587 c, g dbSNP:759960481
618 618 a, c dbSNP:552363141
621 621 a, g dbSNP:753130938
622 622 a, g, t dbSNP:758652888
648 648 a, g dbSNP:369510319
652 652 c, t dbSNP:757398208
653 653 a, g dbSNP:570927821
656 656 a, g, t dbSNP:373646181
657 657 g, t dbSNP:778731236
664 664 c, t dbSNP:747910356
670 670 c, t dbSNP:771906789
695 695 a, g dbSNP:772842161
710 710 a, g dbSNP:746735395
717 717 a, c dbSNP:140654183
721 721 a, c, g dbSNP:770403685
734 734 a, g dbSNP:760036765
748 748 g, t dbSNP:765691650
752 752 c, t dbSNP:180710258
754 754 c, t dbSNP:763385232
756 756 a, t dbSNP:11574936
769 769 c, g dbSNP:751765776
772 772 c, t dbSNP:757435299
795 795 a, g dbSNP:376368223
799 799 c, t dbSNP:750502134
802 802 c, t dbSNP:756260984
803 803 a, g dbSNP:778803751
805 805 c, t dbSNP:748145803
806 806 a, g dbSNP:758189183
807 807 a, g dbSNP:777545571
815 815 c, t dbSNP:141307820
816 816 a, g dbSNP:770509340
820 820 a, c, t dbSNP:780711335
821 821 a, g, t dbSNP:370893734
825 825 a, c dbSNP:763621932
829 829 c, t dbSNP:769084971
838 838 c, t dbSNP:774879769
844 844 g, t dbSNP:762056461
851 851 a, g dbSNP:186486397
853 853 c, g dbSNP:767604552
880 880 a, g dbSNP:750709123
881 881 a, g dbSNP:190947828
882 882 a, g dbSNP:766515527
884 884 c, t dbSNP:752568182
885 885 a, g dbSNP:758342189
888 888 c, t dbSNP:777492465
895 895 c, g dbSNP:535723784
905 905 c, t dbSNP:372252268
906 906 a, g dbSNP:557263022
918 918 c, t dbSNP:780684380
921 921 a, g dbSNP:745590537
929 929 a, g dbSNP:769422678
933 933 c, t dbSNP:575712826
936 936 c, t dbSNP:749764028
937 937 c, t dbSNP:769320071
940 940 c, t dbSNP:775041173
947 947 c, t dbSNP:762064944
949 949 c, t dbSNP:772549106
954 954 a, c dbSNP:3093665
959 959 c, t dbSNP:760959992
979 979 c, t dbSNP:766384108
994 994 a, c dbSNP:753933440
995 995 a, g dbSNP:762834115
1005 1005 c, t dbSNP:763985399
1019 1019 c, g dbSNP:763152978
1023 1023 a, g dbSNP:751562173
1037 1037 a, g dbSNP:756986997
1048 1048 c, t dbSNP:753952568
1065 1065 g, t dbSNP:767411749
1101 1101 a, g dbSNP:757409861
1111 1111 c, t dbSNP:4645845
1193 1193 c, t dbSNP:79207063
1194 1194 c, t dbSNP:10947218
1210 1210 a, g dbSNP:760929534
1226 1226 a, g dbSNP:541176107
1255 1255 -, t dbSNP:55691552
1296 1296 c, t dbSNP:3093666
1330 1330 g, t dbSNP:3093667
1391 1391 g, t dbSNP:28501663
1461 1461 c, t dbSNP:548471164
1486 1486 a, t dbSNP:55788986
1547 1547 a, g dbSNP:556174575
1577 1577 a, c dbSNP:191617007
1608 1608 c, t dbSNP:746164622
1630 1630 a, c dbSNP:552358707

Target ORF information:

RefSeq Version NM_000594
Organism Homo sapiens (human)
Definition Homo sapiens tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000594

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGGCGCT CCCCAAGAAG 
ACAGGGGGGC CCCAGGGCTC CAGGCGGTGC TTGTTCCTCA GCCTCTTCTC CTTCCTGATC
GTGGCAGGCG CCACCACGCT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG
GAAGAGTTCC CCAGGGACCT CTCTCTAATC AGCCCTCTGG CCCAGGCAGT CAGATCATCT
TCTCGAACCC CGAGTGACAA GCCTGTAGCC CATGTTGTAG CAAACCCTCA AGCTGAGGGG
CAGCTCCAGT GGCTGAACCG CCGGGCCAAT GCCCTCCTGG CCAATGGCGT GGAGCTGAGA
GATAACCAGC TGGTGGTGCC ATCAGAGGGC CTGTACCTCA TCTACTCCCA GGTCCTCTTC
AAGGGCCAAG GCTGCCCCTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCATCGCC
GTCTCCTACC AGACCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCCTG CCAGAGGGAG
ACCCCAGAGG GGGCTGAGGC CAAGCCCTGG TATGAGCCCA TCTATCTGGG AGGGGTCTTC
CAGCTGGAGA AGGGTGACCG ACTCAGCGCT GAGATCAATC GGCCCGACTA TCTCGACTTT
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.