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TNF cDNA ORF clone, Homo sapiens (human)

Gene Symbol TNF
Entrez Gene ID 7124
Full Name tumor necrosis factor
Synonyms DIF, TNF-alpha, TNFA, TNFSF2
General protein information
Preferred Names
tumor necrosis factor
Names
tumor necrosis factor
TNF-a
cachectin
APC1 protein
TNF, monocyte-derived
TNF, macrophage-derived
tumor necrosis factor-alpha
tumor necrosis factor ligand superfamily member 2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.3

Summary This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

191160

Disorder Html: {Malaria, cerebral, susceptibility to}, 611162 (3); {Septic shock,

mRNA and Protein(s)

mRNA Protein Name
NM_000594 NP_000585 tumor necrosis factor

KEGG
hsa04150 mTOR signaling pathway
hsa04350 TGF-beta signaling pathway
hsa04664 Fc epsilon RI signaling pathway
hsa04650 Natural killer cell mediated cytotoxicity
hsa04210 Apoptosis
hsa04060 Cytokine-cytokine receptor interaction
hsa04010 MAPK signaling pathway
hsa04640 Hematopoietic cell lineage
hsa04660 T cell receptor signaling pathway
hsa04940 Type I diabetes mellitus
hsa04612 Antigen processing and presentation
hsa04620 Toll-like receptor signaling pathway
hsa05010 Alzheimer's disease
hsa04920 Adipocytokine signaling pathway
hsa05014 Amyotrophic lateral sclerosis (ALS)
hsa05310 Asthma
hsa05322 Systemic lupus erythematosus
hsa04930 Type II diabetes mellitus
hsa05332 Graft-versus-host disease
hsa05330 Allograft rejection
hsa05410 Hypertrophic cardiomyopathy (HCM)
hsa05414 Dilated cardiomyopathy
hsa04621 NOD-like receptor signaling pathway
hsa04622 RIG-I-like receptor signaling pathway
hsa05142 Chagas disease (American trypanosomiasis)
hsa05140 Leishmaniasis
hsa05146 Amoebiasis
hsa05145 Toxoplasmosis
hsa05144 Malaria
hsa05160 Hepatitis C
hsa04380 Osteoclast differentiation
hsa05323 Rheumatoid arthritis
hsa05143 African trypanosomiasis
hsa05152 Tuberculosis
hsa05133 Pertussis
hsa05166 HTLV-I infection
hsa05164 Influenza A
hsa05168 Herpes simplex infection
hsa05134 Legionellosis
hsa05205 Proteoglycans in cancer
hsa05161 Hepatitis B
hsa04064 NF-kappa B signaling pathway
hsa05321 Inflammatory bowel disease (IBD)
hsa04071 Sphingolipid signaling pathway
hsa04668 TNF signaling pathway
hsa04932 Non-alcoholic fatty liver disease (NAFLD)
Reactome
R-HSA-168256 Immune System
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-5668541 TNFR2 non-canonical NF-kB pathway
R-HSA-162582 Signal Transduction
R-HSA-5357786 TNFR1-induced proapoptotic signaling
R-HSA-73887 Death Receptor Signalling
R-HSA-75893 TNF signaling
R-HSA-1266738 Developmental Biology
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
Pathway Interaction Database
il27pathway IL27-mediated signaling events
caspase_pathway Caspase cascade in apoptosis
amb2_neutrophils_pathway amb2 Integrin signaling
angiopoietinreceptor_pathway Angiopoietin receptor Tie2-mediated signaling
rxr_vdr_pathway RXR and RAR heterodimerization with other nuclear receptor
hivnefpathway HIV-1 Nef: Negative effector of Fas and TNF-alpha
ceramide_pathway Ceramide signaling pathway
nfkappabcanonicalpathway Canonical NF-kappaB pathway
hdac_classi_pathway Signaling events mediated by HDAC Class I
nfat_tfpathway Calcineurin-regulated NFAT-dependent transcription in lymphocytes
il23pathway IL23-mediated signaling events
cd8tcrdownstreampathway Downstream signaling in naive CD8+ T cells
tnfpathway TNF receptor signaling pathway
anthraxpathway Cellular roles of Anthrax toxin
WikiPathways
WP382 MAPK signaling pathway
WP530 Cytokines and Inflammatory Response
WP1584 Type II diabetes mellitus
WP560 TGF Beta Signaling Pathway
WP262 EBV LMP1 signaling
WP254 Apoptosis
WP1544 MicroRNAs in cardiomyocyte hypertrophy
WP15 Selenium Pathway
WP727 Monoamine Transport
WP75 Toll-like receptor signaling pathway
WP314 FAS pathway and Stress induction of HSP regulation
WP231 TNF-alpha/NF-kB Signaling Pathway
WP706 SIDS Susceptibility Pathways
WP129 Matrix Metalloproteinases
WP236 Adipogenesis
WP268 Notch Signaling Pathway
WP2377 Integrated Pancreatic Cancer Pathway
WP2100 AhR pathway
WP2036 TWEAK Signaling Pathway
WP2059 Alzheimers Disease
WP2431 Spinal Cord Injury
WP2447 Amyotrophic lateral sclerosis (ALS)
WP1449 Regulation of toll-like receptor signaling pathway
WP2328 Allograft Rejection


Homo sapiens (human) TNF NP_000585.2
Pan troglodytes (chimpanzee) TNF NP_001038976.1
Macaca mulatta (Rhesus monkey) TNF NP_001040614.1
Canis lupus familiaris (dog) TNF NP_001003244.4
Bos taurus (cattle) TNF NP_776391.2
Mus musculus (house mouse) Tnf NP_038721.1
Rattus norvegicus (Norway rat) Tnf NP_036807.1
Xenopus (Silurana) tropicalis (western clawed frog) tnf NP_001107143.1

Component

ID Name Evidence
GO:0001891 phagocytic cup ISS
GO:0005576 extracellular region IEA
GO:0005615 extracellular space IDA
GO:0005622 intracellular IEA
GO:0005886 plasma membrane EXP
GO:0005887 integral to plasma membrane IDA
GO:0009897 external side of plasma membrane ISS
GO:0009986 cell surface IDA
GO:0045121 membrane raft IDA
GO:0055037 recycling endosome ISS

Function

ID Name Evidence
GO:0002020 protease binding IPI
GO:0005125 cytokine activity IDA
GO:0005164 tumor necrosis factor receptor binding IDA
GO:0005515 protein binding IPI
GO:0042802 identical protein binding IDA

Process

ID Name Evidence
GO:0000060 protein import into nucleus, translocation IDA
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IEA
GO:0000185 activation of MAPKKK activity IDA
GO:0000187 activation of MAPK activity IDA
GO:0001666 response to hypoxia IEA
GO:0001775 cell activation IEA
GO:0001819 positive regulation of cytokine production IDA
GO:0001934 positive regulation of protein phosphorylation IDA
GO:0002037 negative regulation of L-glutamate transport IEA
GO:0002439 chronic inflammatory response to antigenic stimulus IMP
GO:0002740 negative regulation of cytokine secretion involved in immune response IDA
GO:0002876 positive regulation of chronic inflammatory response to antigenic stimulus IEA
GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin IEA
GO:0003009 skeletal muscle contraction IEA
GO:0006006 glucose metabolic process IEA
GO:0006915 apoptosis TAS
GO:0006916 anti-apoptosis IDA
GO:0006917 induction of apoptosis IDA
GO:0006919 activation of caspase activity IDA
GO:0006927 transformed cell apoptosis IDA
GO:0006954 inflammatory response IDA
GO:0006959 humoral immune response IEA
GO:0007165 signal transduction IEA
GO:0007275 multicellular organismal development IEA
GO:0008285 negative regulation of cell proliferation IEA
GO:0008624 induction of apoptosis by extracellular signals IDA
GO:0008624 induction of apoptosis by extracellular signals TAS
GO:0008625 induction of apoptosis via death domain receptors IDA
GO:0009612 response to mechanical stimulus IEA
GO:0009615 response to virus IDA
GO:0009651 response to salt stress TAS
GO:0009887 organ morphogenesis IEA
GO:0010888 negative regulation of lipid storage NAS
GO:0010940 positive regulation of necrotic cell death TAS
GO:0014823 response to activity IEA
GO:0019722 calcium-mediated signaling IEA
GO:0030198 extracellular matrix organization IEA
GO:0030730 sequestering of triglyceride IDA
GO:0031334 positive regulation of protein complex assembly IDA
GO:0031622 positive regulation of fever generation ISS
GO:0032715 negative regulation of interleukin-6 production IDA
GO:0032722 positive regulation of chemokine production IDA
GO:0032729 positive regulation of interferon-gamma production IEA
GO:0032755 positive regulation of interleukin-6 production IEA
GO:0032800 receptor biosynthetic process IDA
GO:0033138 positive regulation of peptidyl-serine phosphorylation IDA
GO:0033209 tumor necrosis factor-mediated signaling pathway IMP
GO:0034116 positive regulation of heterotypic cell-cell adhesion IDA
GO:0042346 positive regulation of NF-kappaB import into nucleus IDA
GO:0042493 response to drug IEA
GO:0043066 negative regulation of apoptosis IDA
GO:0043122 regulation of I-kappaB kinase/NF-kappaB cascade IDA
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade IDA
GO:0043243 positive regulation of protein complex disassembly IDA
GO:0043406 positive regulation of MAP kinase activity IDA
GO:0043525 positive regulation of neuron apoptosis IEA
GO:0044130 negative regulation of growth of symbiont in host IEA
GO:0045071 negative regulation of viral genome replication IDA
GO:0045080 positive regulation of chemokine biosynthetic process IDA
GO:0045429 positive regulation of nitric oxide biosynthetic process IDA
GO:0045599 negative regulation of fat cell differentiation NAS
GO:0045672 positive regulation of osteoclast differentiation IDA
GO:0045840 positive regulation of mitosis IEA
GO:0045892 negative regulation of transcription, DNA-dependent IDA
GO:0045893 positive regulation of transcription, DNA-dependent IDA
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IGI
GO:0045994 positive regulation of translational initiation by iron IEA
GO:0046325 negative regulation of glucose import IEA
GO:0046330 positive regulation of JNK cascade IEA
GO:0048566 embryonic digestive tract development IEP
GO:0048661 positive regulation of smooth muscle cell proliferation IDA
GO:0050715 positive regulation of cytokine secretion IDA
GO:0050796 regulation of insulin secretion IDA
GO:0050806 positive regulation of synaptic transmission IEA
GO:0050830 defense response to Gram-positive bacterium IEA
GO:0050901 leukocyte tethering or rolling IDA
GO:0050995 negative regulation of lipid catabolic process IDA
GO:0051023 regulation of immunoglobulin secretion IEA
GO:0051044 positive regulation of membrane protein ectodomain proteolysis IDA
GO:0051091 positive regulation of sequence-specific DNA binding transcription factor activity IDA
GO:0051092 positive regulation of NF-kappaB transcription factor activity IDA
GO:0051222 positive regulation of protein transport IDA
GO:0051384 response to glucocorticoid stimulus IDA
GO:0051533 positive regulation of NFAT protein import into nucleus IDA
GO:0051798 positive regulation of hair follicle development IEA
GO:0060555 induction of necroptosis by extracellular signals IDA
GO:0060557 positive regulation of vitamin D biosynthetic process IDA
GO:0060559 positive regulation of calcidiol 1-monooxygenase activity IDA
GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis IEA
GO:0060693 regulation of branching involved in salivary gland morphogenesis IEA
GO:0061048 negative regulation of branching involved in lung morphogenesis IDA
GO:0070265 necrotic cell death IDA
GO:0070374 positive regulation of ERK1 and ERK2 cascade NAS
GO:0071316 cellular response to nicotine IDA
GO:0071803 positive regulation of podosome assembly IDA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27508 NM_000594 Homo sapiens tumor necrosis factor (TNF), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu27508
Accession Version NM_000594.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP215875.1, BC028148.1 and CA306559.1. This sequence is a reference standard in the RefSeqGene project. On Jul 12, 2012 this sequence version replaced gi:25952110. Summary: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP215875.1, BC028148.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGGCGCT CCCCAAGAAG 
ACAGGGGGGC CCCAGGGCTC CAGGCGGTGC TTGTTCCTCA GCCTCTTCTC CTTCCTGATC
GTGGCAGGCG CCACCACGCT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG
GAAGAGTTCC CCAGGGACCT CTCTCTAATC AGCCCTCTGG CCCAGGCAGT CAGATCATCT
TCTCGAACCC CGAGTGACAA GCCTGTAGCC CATGTTGTAG CAAACCCTCA AGCTGAGGGG
CAGCTCCAGT GGCTGAACCG CCGGGCCAAT GCCCTCCTGG CCAATGGCGT GGAGCTGAGA
GATAACCAGC TGGTGGTGCC ATCAGAGGGC CTGTACCTCA TCTACTCCCA GGTCCTCTTC
AAGGGCCAAG GCTGCCCCTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCATCGCC
GTCTCCTACC AGACCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCCTG CCAGAGGGAG
ACCCCAGAGG GGGCTGAGGC CAAGCCCTGG TATGAGCCCA TCTATCTGGG AGGGGTCTTC
CAGCTGGAGA AGGGTGACCG ACTCAGCGCT GAGATCAATC GGCCCGACTA TCTCGACTTT
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_000585.2
CDS176..877
Misc Feature(1)98..100(+)
Misc Feature(2)179..181(+)
Misc Feature(3)230..232(+)
Misc Feature(4)233..235(+)
Misc Feature(5)278..283(+)
Misc Feature(6)281..343(+)
Misc Feature(7)290..295(+)
Misc Feature(8)320..325(+)
Misc Feature(9)326..331(+)
Misc Feature(10)395..397(+)
Misc Feature(11)395..397(+)
Misc Feature(12)395..397(+)
Misc Feature(13)401..406(+)
Misc Feature(14)401..403(+)
Misc Feature(15)401..403(+)
Misc Feature(16)410..412(+)
Misc Feature(17)437..868(+)
Misc Feature(18)446..868(+)
Misc Feature(19)488..670(+)
Exon (1)1..361
Gene:TNF
Gene Synonym:
Exon (2)362..407
Gene:TNF
Gene Synonym:
Exon (3)408..455
Gene:TNF
Gene Synonym:
Exon (4)456..1676
Gene:TNF
Gene Synonym:
Translation
Position Chain Variation Link
5 5 c, t dbSNP:4248164
31 31 a, g dbSNP:61761335
39 39 -, ag dbSNP:4647199
61 61 -, c dbSNP:201328097
63 63 c, g dbSNP:3093660
68 68 -, c dbSNP:4645838
68 68 -, c dbSNP:386594198
116 116 a, g dbSNP:538552423
133 133 a, t dbSNP:372568141
143 143 c, t dbSNP:752785007
144 144 a, g dbSNP:554060079
146 146 c, t dbSNP:372719825
148 148 c, t dbSNP:2515924
149 149 a, c dbSNP:750426362
152 152 a, c dbSNP:755847921
160 160 c, t dbSNP:779966486
170 170 a, g dbSNP:375864945
177 177 c, g, t dbSNP:369780852
188 188 a, c dbSNP:747694851
189 189 a, g dbSNP:771650207
190 190 c, t dbSNP:565862537
197 197 c, t dbSNP:374531985
198 198 a, g dbSNP:201502336
202 202 c, t dbSNP:377627218
203 203 a, g dbSNP:759696973
205 205 a, g dbSNP:770023902
215 215 a, g dbSNP:775506502
222 222 c, g, t dbSNP:763000109
223 223 a, g dbSNP:146375573
231 231 a, g dbSNP:377338702
238 238 a, c dbSNP:766034823
239 239 a, g dbSNP:753713562
245 245 c, g dbSNP:754639425
248 248 c, g dbSNP:764955335
259 259 a, g dbSNP:779870893
261 261 a, g dbSNP:576621666
262 262 g, t dbSNP:2228088
270 270 c, t dbSNP:777405140
272 272 c, t dbSNP:747406665
287 287 c, t dbSNP:757794184
295 295 a, c, t dbSNP:141667614
296 296 a, g, t dbSNP:558678940
304 304 c, t dbSNP:369163834
312 312 c, t dbSNP:200241887
313 313 a, g dbSNP:144480538
316 316 c, t dbSNP:541347219
329 329 a, c dbSNP:3179060
336 336 a, g dbSNP:756182468
344 344 a, g dbSNP:777874746
346 346 c, t dbSNP:776753689
358 358 a, g dbSNP:759378982
360 360 a, g dbSNP:765178016
366 366 c, t dbSNP:35131721
367 367 c, t dbSNP:772331441
371 371 a, g dbSNP:776692566
380 380 c, t dbSNP:759609722
400 400 a, g dbSNP:769643681
407 407 a, g dbSNP:775365890
413 413 c, t dbSNP:369783162
420 420 a, g dbSNP:772270241
426 426 c, t dbSNP:4645843
427 427 a, g dbSNP:745738344
435 435 a, c dbSNP:190788828
455 455 a, g dbSNP:1800620
467 467 a, g dbSNP:375472217
481 481 c, t dbSNP:1800618
495 495 a, g dbSNP:758704433
497 497 c, t dbSNP:281865419
498 498 a, g dbSNP:747115001
502 502 a, c dbSNP:771174703
516 516 a, c dbSNP:780017660
520 520 c, t dbSNP:142488339
524 524 a, g dbSNP:548532642
525 525 c, t dbSNP:774032100
529 529 a, g dbSNP:747751043
534 534 a, g dbSNP:778657377
535 535 a, g dbSNP:569852154
556 556 a, g dbSNP:751421650
561 561 a, g dbSNP:772893367
562 562 a, g dbSNP:537268309
565 565 c, t dbSNP:150508976
574 574 c, t dbSNP:777187968
587 587 c, g dbSNP:759960481
618 618 a, c dbSNP:552363141
621 621 a, g dbSNP:753130938
622 622 a, g, t dbSNP:758652888
648 648 a, g dbSNP:369510319
652 652 c, t dbSNP:757398208
653 653 a, g dbSNP:570927821
656 656 a, g, t dbSNP:373646181
657 657 g, t dbSNP:778731236
664 664 c, t dbSNP:747910356
670 670 c, t dbSNP:771906789
695 695 a, g dbSNP:772842161
710 710 a, g dbSNP:746735395
717 717 a, c dbSNP:140654183
721 721 a, c, g dbSNP:770403685
734 734 a, g dbSNP:760036765
748 748 g, t dbSNP:765691650
752 752 c, t dbSNP:180710258
754 754 c, t dbSNP:763385232
756 756 a, t dbSNP:11574936
769 769 c, g dbSNP:751765776
772 772 c, t dbSNP:757435299
795 795 a, g dbSNP:376368223
799 799 c, t dbSNP:750502134
802 802 c, t dbSNP:756260984
803 803 a, g dbSNP:778803751
805 805 c, t dbSNP:748145803
806 806 a, g dbSNP:758189183
807 807 a, g dbSNP:777545571
815 815 c, t dbSNP:141307820
816 816 a, g dbSNP:770509340
820 820 a, c, t dbSNP:780711335
821 821 a, g, t dbSNP:370893734
825 825 a, c dbSNP:763621932
829 829 c, t dbSNP:769084971
838 838 c, t dbSNP:774879769
844 844 g, t dbSNP:762056461
851 851 a, g dbSNP:186486397
853 853 c, g dbSNP:767604552
880 880 a, g dbSNP:750709123
881 881 a, g dbSNP:190947828
882 882 a, g dbSNP:766515527
884 884 c, t dbSNP:752568182
885 885 a, g dbSNP:758342189
888 888 c, t dbSNP:777492465
895 895 c, g dbSNP:535723784
905 905 c, t dbSNP:372252268
906 906 a, g dbSNP:557263022
918 918 c, t dbSNP:780684380
921 921 a, g dbSNP:745590537
929 929 a, g dbSNP:769422678
933 933 c, t dbSNP:575712826
936 936 c, t dbSNP:749764028
937 937 c, t dbSNP:769320071
940 940 c, t dbSNP:775041173
947 947 c, t dbSNP:762064944
949 949 c, t dbSNP:772549106
954 954 a, c dbSNP:3093665
959 959 c, t dbSNP:760959992
979 979 c, t dbSNP:766384108
994 994 a, c dbSNP:753933440
995 995 a, g dbSNP:762834115
1005 1005 c, t dbSNP:763985399
1019 1019 c, g dbSNP:763152978
1023 1023 a, g dbSNP:751562173
1037 1037 a, g dbSNP:756986997
1048 1048 c, t dbSNP:753952568
1065 1065 g, t dbSNP:767411749
1101 1101 a, g dbSNP:757409861
1111 1111 c, t dbSNP:4645845
1193 1193 c, t dbSNP:79207063
1194 1194 c, t dbSNP:10947218
1210 1210 a, g dbSNP:760929534
1226 1226 a, g dbSNP:541176107
1255 1255 -, t dbSNP:55691552
1296 1296 c, t dbSNP:3093666
1330 1330 g, t dbSNP:3093667
1391 1391 g, t dbSNP:28501663
1461 1461 c, t dbSNP:548471164
1486 1486 a, t dbSNP:55788986
1547 1547 a, g dbSNP:556174575
1577 1577 a, c dbSNP:191617007
1608 1608 c, t dbSNP:746164622
1630 1630 a, c dbSNP:552358707

Target ORF information:

RefSeq Version NM_000594
Organism Homo sapiens (human)
Definition Homo sapiens tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000594

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGGCGCT CCCCAAGAAG 
ACAGGGGGGC CCCAGGGCTC CAGGCGGTGC TTGTTCCTCA GCCTCTTCTC CTTCCTGATC
GTGGCAGGCG CCACCACGCT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG
GAAGAGTTCC CCAGGGACCT CTCTCTAATC AGCCCTCTGG CCCAGGCAGT CAGATCATCT
TCTCGAACCC CGAGTGACAA GCCTGTAGCC CATGTTGTAG CAAACCCTCA AGCTGAGGGG
CAGCTCCAGT GGCTGAACCG CCGGGCCAAT GCCCTCCTGG CCAATGGCGT GGAGCTGAGA
GATAACCAGC TGGTGGTGCC ATCAGAGGGC CTGTACCTCA TCTACTCCCA GGTCCTCTTC
AAGGGCCAAG GCTGCCCCTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCATCGCC
GTCTCCTACC AGACCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCCTG CCAGAGGGAG
ACCCCAGAGG GGGCTGAGGC CAAGCCCTGG TATGAGCCCA TCTATCTGGG AGGGGTCTTC
CAGCTGGAGA AGGGTGACCG ACTCAGCGCT GAGATCAATC GGCCCGACTA TCTCGACTTT
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Sine oculis homeobox homolog 1 promotes alpha5beta1-mediated invasive migration and metastasis of cervical cancer cells
Biochem. Biophys. Res. Commun. 446 (2), 549-554 (2014)
Liu D, Zhang XX, Wan DY, Xi BX, Ma D, Wang H and Gao QL.


book

Lack of association between TNF-alpha promoter polymorphism and prostate carcinoma susceptibility in eastern Croatian population
Coll Antropol 37 (4), 1199-1202 (2013)
Horvat V, Sudarevic B, Cosic I, Milicevic N, Bosnjak S, Mandic S, Marczi S, Simunovic D and Galic J.


book

CTLA-4 +49 and TNF-alpha-308 gene polymorphisms in celiac patients with exocrine pancreatic insufficiency
Coll Antropol 37 (4), 1191-1194 (2013)
Licul V, Cizmarevic NS, Ristic S, Mikolasevic I and Mijandrusic BS.


book

Polymorphisms of the IL12B, IL1B, and TNFA genes and susceptibility to asthma
J Investig Allergol Clin Immunol 23 (7), 487-494 (2013)
Padron-Morales,J., Sanz,C., Davila,I., Munoz-Bellido,F., Lorente,F. and Isidoro-Garcia,M.


book

Single-nucleotide polymorphisms of TNFA and IL1 in allergic rhinitis
J Investig Allergol Clin Immunol 23 (7), 455-461 (2013)
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