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TNF cDNA ORF clone, Homo sapiens (human)

Gene Symbol TNF
Entrez Gene ID 7124
Full Name tumor necrosis factor
Synonyms DIF, TNF-alpha, TNFA, TNFSF2
General protein information
Preferred Names
tumor necrosis factor
Names
tumor necrosis factor
TNF-a
cachectin
APC1 protein
TNF, monocyte-derived
TNF, macrophage-derived
tumor necrosis factor-alpha
tumor necrosis factor ligand superfamily member 2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.3

Summary This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

191160

Disorder Html: {Malaria, cerebral, susceptibility to}, 611162 (3); {Septic shock,

mRNA and Protein(s)

mRNA Protein Name
NM_000594 NP_000585 tumor necrosis factor



Homo sapiens (human) TNF NP_000585.2
Pan troglodytes (chimpanzee) TNF NP_001038976.1
Macaca mulatta (Rhesus monkey) TNF NP_001040614.1
Canis lupus familiaris (dog) TNF NP_001003244.4
Bos taurus (cattle) TNF NP_776391.2
Mus musculus (house mouse) Tnf NP_038721.1
Rattus norvegicus (Norway rat) Tnf NP_036807.1
Xenopus (Silurana) tropicalis (western clawed frog) tnf NP_001107143.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27508 NM_000594 Homo sapiens tumor necrosis factor (TNF), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27508
Clone ID Related Accession (Same CDS sequence) NM_000594
Accession Version NM_000594.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP215875.1, BC028148.1 and CA306559.1. This sequence is a reference standard in the RefSeqGene project. On Jul 12, 2012 this sequence version replaced gi:25952110. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP215875.1, BC028148.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGGCGCT CCCCAAGAAG 
ACAGGGGGGC CCCAGGGCTC CAGGCGGTGC TTGTTCCTCA GCCTCTTCTC CTTCCTGATC
GTGGCAGGCG CCACCACGCT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG
GAAGAGTTCC CCAGGGACCT CTCTCTAATC AGCCCTCTGG CCCAGGCAGT CAGATCATCT
TCTCGAACCC CGAGTGACAA GCCTGTAGCC CATGTTGTAG CAAACCCTCA AGCTGAGGGG
CAGCTCCAGT GGCTGAACCG CCGGGCCAAT GCCCTCCTGG CCAATGGCGT GGAGCTGAGA
GATAACCAGC TGGTGGTGCC ATCAGAGGGC CTGTACCTCA TCTACTCCCA GGTCCTCTTC
AAGGGCCAAG GCTGCCCCTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCATCGCC
GTCTCCTACC AGACCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCCTG CCAGAGGGAG
ACCCCAGAGG GGGCTGAGGC CAAGCCCTGG TATGAGCCCA TCTATCTGGG AGGGGTCTTC
CAGCTGGAGA AGGGTGACCG ACTCAGCGCT GAGATCAATC GGCCCGACTA TCTCGACTTT
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000585.2
CDS176..877
Misc Feature(1)98..100(+)
Misc Feature(2)179..181(+)
Misc Feature(3)230..232(+)
Misc Feature(4)233..235(+)
Misc Feature(5)278..283(+)
Misc Feature(6)281..343(+)
Misc Feature(7)290..295(+)
Misc Feature(8)320..325(+)
Misc Feature(9)326..331(+)
Misc Feature(10)395..397(+)
Misc Feature(11)395..397(+)
Misc Feature(12)395..397(+)
Misc Feature(13)401..406(+)
Misc Feature(14)401..403(+)
Misc Feature(15)401..403(+)
Misc Feature(16)410..412(+)
Misc Feature(17)437..868(+)
Misc Feature(18)446..868(+)
Misc Feature(19)488..670(+)
Exon (1)1..361
Gene:TNF
Gene Synonym:
Exon (2)362..407
Gene:TNF
Gene Synonym:
Exon (3)408..455
Gene:TNF
Gene Synonym:
Exon (4)456..1676
Gene:TNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000594
Organism Homo sapiens (human)
Definition Homo sapiens tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000594

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGGCGCT CCCCAAGAAG 
ACAGGGGGGC CCCAGGGCTC CAGGCGGTGC TTGTTCCTCA GCCTCTTCTC CTTCCTGATC
GTGGCAGGCG CCACCACGCT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG
GAAGAGTTCC CCAGGGACCT CTCTCTAATC AGCCCTCTGG CCCAGGCAGT CAGATCATCT
TCTCGAACCC CGAGTGACAA GCCTGTAGCC CATGTTGTAG CAAACCCTCA AGCTGAGGGG
CAGCTCCAGT GGCTGAACCG CCGGGCCAAT GCCCTCCTGG CCAATGGCGT GGAGCTGAGA
GATAACCAGC TGGTGGTGCC ATCAGAGGGC CTGTACCTCA TCTACTCCCA GGTCCTCTTC
AAGGGCCAAG GCTGCCCCTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCATCGCC
GTCTCCTACC AGACCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCCTG CCAGAGGGAG
ACCCCAGAGG GGGCTGAGGC CAAGCCCTGG TATGAGCCCA TCTATCTGGG AGGGGTCTTC
CAGCTGGAGA AGGGTGACCG ACTCAGCGCT GAGATCAATC GGCCCGACTA TCTCGACTTT
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Sine oculis homeobox homolog 1 promotes alpha5beta1-mediated invasive migration and metastasis of cervical cancer cells
Biochem. Biophys. Res. Commun. 446 (2), 549-554 (2014)
Liu D, Zhang XX, Wan DY, Xi BX, Ma D, Wang H and Gao QL.


book

Lack of association between TNF-alpha promoter polymorphism and prostate carcinoma susceptibility in eastern Croatian population
Coll Antropol 37 (4), 1199-1202 (2013)
Horvat V, Sudarevic B, Cosic I, Milicevic N, Bosnjak S, Mandic S, Marczi S, Simunovic D and Galic J.


book

CTLA-4 +49 and TNF-alpha-308 gene polymorphisms in celiac patients with exocrine pancreatic insufficiency
Coll Antropol 37 (4), 1191-1194 (2013)
Licul V, Cizmarevic NS, Ristic S, Mikolasevic I and Mijandrusic BS.


book

Polymorphisms of the IL12B, IL1B, and TNFA genes and susceptibility to asthma
J Investig Allergol Clin Immunol 23 (7), 487-494 (2013)
Padron-Morales,J., Sanz,C., Davila,I., Munoz-Bellido,F., Lorente,F. and Isidoro-Garcia,M.


book

Single-nucleotide polymorphisms of TNFA and IL1 in allergic rhinitis
J Investig Allergol Clin Immunol 23 (7), 455-461 (2013)
Nasiri,R., Amirzargar,A.A., Movahedi,M., Hirbod-Mobarakeh,A., Farhadi,E., Behniafard,N., Tavakkol,M., Ansaripour,B., Moradi,B., Zare,A. and Rezaei,N.


book

Effects of the human immunodeficiency virus type 1 Tat protein on the expression of inflammatory cytokines
J. Virol. 66 (12), 7159-7167 (1992)
Buonaguro L, Barillari G, Chang HK, Bohan CA, Kao V, Morgan R, Gallo RC and Ensoli B.


book

Site-directed mutational analysis of human tumor necrosis factor-alpha receptor binding site and structure-functional relationship
J. Biol. Chem. 267 (33), 24069-24075 (1992)
Zhang XM, Weber I and Chen MJ.


book

Myristyl acylation of the tumor necrosis factor alpha precursor on specific lysine residues
J. Exp. Med. 176 (4), 1053-1062 (1992)
Stevenson FT, Bursten SL, Locksley RM and Lovett DH.


book

Genomic structure, induction, and production of TNF-alpha
Immunol. Ser. 56, 3-34 (1992)
Spriggs DR, Deutsch S and Kufe DW.


book

Tumor necrosis factor-alpha induces vitamin D-1-hydroxylase activity in normal human alveolar macrophages
J. Cell. Physiol. 142 (3), 652-656 (1990)
Pryke AM, Duggan C, White CP, Posen S and Mason RS.


 
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