×

TNNI3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol TNNI3
Entrez Gene ID 7137
Full Name troponin I type 3 (cardiac)
Synonyms CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI
General protein information
Preferred Names
troponin I, cardiac muscle
Names
troponin I, cardiac muscle
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.4

Summary Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

191044

Disorder Html: Cardiomyopathy, familial hypertrophic, 7, 613690 (3); Cardiomyopathy,

mRNA and Protein(s)

mRNA Protein Name
NM_000363 NP_000354 troponin I, cardiac muscle



Homo sapiens (human) TNNI3 NP_000354.4
Pan troglodytes (chimpanzee) TNNI3 XP_001134934.2
Macaca mulatta (Rhesus monkey) TNNI3 XP_001085820.1
Canis lupus familiaris (dog) TNNI3 NP_001003041.1
Bos taurus (cattle) LOC511094 NP_001035607.1
Mus musculus (house mouse) Tnni3 NP_033432.1
Rattus norvegicus (Norway rat) Tnni3 NP_058840.1
Xenopus (Silurana) tropicalis (western clawed frog) tnni3 NP_001011410.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TNNI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNNI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25717
NM_000363 Homo sapiens troponin I type 3 (cardiac) (TNNI3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25717
Clone ID Related Accession (Same CDS sequence) NM_000363
Accession Version NM_000363.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 633bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product troponin I, cardiac muscle
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK223451.1. This sequence is a reference standard in the RefSeqGene project. On Jul 5, 2007 this sequence version replaced gi:30141909. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223451.1, EU747720.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGCGGATG GGAGCAGCGA TGCGGCTAGG GAACCTCGCC CTGCACCAGC CCCAATCAGA 
CGCCGCTCCT CCAACTACCG CGCTTATGCC ACGGAGCCGC ACGCCAAGAA AAAATCTAAG
ATCTCCGCCT CGAGAAAATT GCAGCTGAAG ACTCTGCTGC TGCAGATTGC AAAGCAAGAG
CTGGAGCGAG AGGCGGAGGA GCGGCGCGGA GAGAAGGGGC GCGCTCTGAG CACCCGCTGC
CAGCCGCTGG AGTTGGCCGG GCTGGGCTTC GCGGAGCTGC AGGACTTGTG CCGACAGCTC
CACGCCCGTG TGGACAAGGT GGATGAAGAG AGATACGACA TAGAGGCAAA AGTCACCAAG
AACATCACGG AGATTGCAGA TCTGACTCAG AAGATCTTTG ACCTTCGAGG CAAGTTTAAG
CGGCCCACCC TGCGGAGAGT GAGGATCTCT GCAGATGCCA TGATGCAGGC GCTGCTGGGG
GCCCGGGCTA AGGAGTCCCT GGACCTGCGG GCCCACCTCA AGCAGGTGAA GAAGGAGGAC
ACCGAGAAGG AAAACCGGGA GGTGGGAGAC TGGCGCAAGA ACATCGATGC ACTGAGTGGA
ATGGAGGGCC GCAAGAAAAA GTTTGAGAGC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000354.4
CDS144..776
Misc Feature(1)144..236(+)
Misc Feature(2)147..149(+)
Misc Feature(3)156..158(+)
Misc Feature(4)159..161(+)
Misc Feature(5)210..212(+)
Misc Feature(6)213..215(+)
Misc Feature(7)219..221(+)
Misc Feature(8)234..236(+)
Misc Feature(9)237..380(+)
Misc Feature(10)294..296(+)
Misc Feature(11)357..623(+)
Misc Feature(12)372..374(+)
Misc Feature(13)375..377(+)
Misc Feature(14)381..383(+)
Misc Feature(15)432..434(+)
Misc Feature(16)528..590(+)
Misc Feature(17)528..530(+)
Misc Feature(18)570..572(+)
Misc Feature(19)591..593(+)
Misc Feature(20)639..641(+)
Misc Feature(21)684..686(+)
Misc Feature(22)738..740(+)
Exon (1)1..154
Gene:TNNI3
Gene Synonym:
Exon (2)155..167
Gene:TNNI3
Gene Synonym:
Exon (3)168..251
Gene:TNNI3
Gene Synonym:
Exon (4)252..293
Gene:TNNI3
Gene Synonym:
Exon (5)294..425
Gene:TNNI3
Gene Synonym:
Exon (6)426..515
Gene:TNNI3
Gene Synonym:
Exon (7)516..692
Gene:TNNI3
Gene Synonym:
Exon (8)693..843
Gene:TNNI3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000363
Organism Homo sapiens (human)
Definition Homo sapiens troponin I type 3 (cardiac) (TNNI3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000363

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCGGATG GGAGCAGCGA TGCGGCTAGG GAACCTCGCC CTGCACCAGC CCCAATCAGA 
CGCCGCTCCT CCAACTACCG CGCTTATGCC ACGGAGCCGC ACGCCAAGAA AAAATCTAAG
ATCTCCGCCT CGAGAAAATT GCAGCTGAAG ACTCTGCTGC TGCAGATTGC AAAGCAAGAG
CTGGAGCGAG AGGCGGAGGA GCGGCGCGGA GAGAAGGGGC GCGCTCTGAG CACCCGCTGC
CAGCCGCTGG AGTTGGCCGG GCTGGGCTTC GCGGAGCTGC AGGACTTGTG CCGACAGCTC
CACGCCCGTG TGGACAAGGT GGATGAAGAG AGATACGACA TAGAGGCAAA AGTCACCAAG
AACATCACGG AGATTGCAGA TCTGACTCAG AAGATCTTTG ACCTTCGAGG CAAGTTTAAG
CGGCCCACCC TGCGGAGAGT GAGGATCTCT GCAGATGCCA TGATGCAGGC GCTGCTGGGG
GCCCGGGCTA AGGAGTCCCT GGACCTGCGG GCCCACCTCA AGCAGGTGAA GAAGGAGGAC
ACCGAGAAGG AAAACCGGGA GGTGGGAGAC TGGCGCAAGA ACATCGATGC ACTGAGTGGA
ATGGAGGGCC GCAAGAAAAA GTTTGAGAGC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Early increases in multiple biomarkers predict subsequent cardiotoxicity in patients with breast cancer treated with doxorubicin, taxanes, and trastuzumab
J. Am. Coll. Cardiol. 63 (8), 809-816 (2014)
Ky B, Putt M, Sawaya H, French B, Januzzi JL Jr, Sebag IA, Plana JC, Cohen V, Banchs J, Carver JR, Wiegers SE, Martin RP, Picard MH, Gerszten RE, Halpern EF, Passeri J, Kuter I and Scherrer-Crosbie M.


book

High-sensitivity troponin I for risk assessment in patients with atrial fibrillation: insights from the Apixaban for Reduction in Stroke and other Thromboembolic Events in Atrial Fibrillation (ARISTOTLE) trial
Circulation 129 (6), 625-634 (2014)
Hijazi Z, Siegbahn A, Andersson U, Granger CB, Alexander JH, Atar D, Gersh BJ, Mohan P, Harjola VP, Horowitz J, Husted S, Hylek EM, Lopes RD, McMurray JJ and Wallentin L.


book

Cardiac troponin I in patients with chronic kidney disease stage 3 to 5 in conditions other than acute coronary syndrome
Clin. Lab. 60 (2), 281-290 (2014)
Flores-Solis,L.M. and Hernandez-Dominguez,J.L.


book

Persistently elevated troponin I in paracetamol hepatotoxicity: association with liver injury, organ failure, and outcome
Clin Toxicol (Phila) 51 (7), 532-539 (2013)
Moore JK, Craig DG, Pryde EA, Walker SW, Beckett GJ, Hayes PC and Simpson KJ.


book

Hypertrophic Cardiomyopathy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Cirino,A.L. and Ho,C.


book

Dilated Cardiomyopathy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Hershberger,R.E. and Morales,A.


book

The human cardiac troponin I locus: assignment to chromosome 19p13.2-19q13.2
Hum. Genet. 88 (1), 101-104 (1991)
MacGeoch C, Barton PJ, Vallins WJ, Bhavsar P and Spurr NK.


book

Troponin I isoform expression in human heart
Circ. Res. 69 (5), 1409-1414 (1991)
Hunkeler NM, Kullman J and Murphy AM.


book

A common motif of two adjacent phosphoserines in bovine, rabbit and human cardiac troponin I
FEBS Lett. 273 (1-2), 41-45 (1990)
Mittmann K, Jaquet K and Heilmeyer LM Jr.


book

Molecular cloning of human cardiac troponin I using polymerase chain reaction
FEBS Lett. 270 (1-2), 57-61 (1990)
Vallins WJ, Brand NJ, Dabhade N, Butler-Browne G, Yacoub MH and Barton PJ.


 
*
*
*
*