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TNNT2 troponin T type 2 (cardiac) [Homo sapiens (human)]

Gene Symbol TNNT2
Entrez Gene ID 7139
Full Name troponin T type 2 (cardiac)
Synonyms CMD1D, CMH2, CMPD2, LVNC6, RCM3, TnTC, cTnT
General protein information
Preferred Names
troponin T, cardiac muscle
Names
troponin T, cardiac muscle
troponin T2, cardiac
cardiomyopathy, hypertrophic 2
cardiomyopathy, dilated 1D (autosomal dominant)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q32

Summary The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

191045

Disorder Html: Cardiomyopathy, familial hypertrophic, 2, 115195 (3); Cardiomyopathy,





The following TNNT2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNNT2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu24970 NM_000364 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $309
OHu24695 NM_001276347 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu25008 NM_001276346 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu25054 NM_001276345 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu24894 NM_001001432 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $309
OHu24695 NM_001001430 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$309
OHu24914 NM_001001431 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$309
OHu25054 XM_011509938 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu61071 XM_011509939 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu61072 XM_011509940 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu61073 XM_011509941 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu24695 XM_006711508 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu34665 XM_006711509 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu34668 XM_011509942 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu34668 XM_011509943 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu34669 XM_011509944 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu61074 XM_011509945 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu61075 XM_011509946 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu24970
Accession Version NM_000364.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 888bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ709280.1, X79855.1, BC002653.2 and AA865519.1. On Feb 5, 2013 this sequence version replaced gi:48255876. Summary: The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) lacks an in-frame exon in the 3' coding region, compared to variant 5. It encodes a shorter isoform (1) compared to isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
ACAGAGCGGA AAAGTGGGAA GAGGCAGACT GAGCGGGAAA AGAAGAAGAA GATTCTGGCT
GAGAGGAGGA AGGTGCTGGC CATTGACCAC CTGAATGAAG ATCAGCTGAG GGAGAAGGCC
AAGGAGCTGT GGCAGAGCAT CTATAACTTG GAGGCAGAGA AGTTCGACCT GCAGGAGAAG
TTCAAGCAGC AGAAATATGA GATCAATGTT CTCCGAAACA GGATCAACGA TAACCAGAAA
GTCTCCAAGA CCCGCGGGAA GGCTAAAGTC ACCGGGCGCT GGAAATAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000355.2
CDS93..980
Misc Feature(1)75..77(+)
Misc Feature(2)96..98(+)
Misc Feature(3)96..98(+)
Misc Feature(4)399..782(+)
Misc Feature(5)693..695(+)
Misc Feature(6)693..695(+)
Misc Feature(7)705..707(+)
Misc Feature(8)705..707(+)
Misc Feature(9)720..722(+)
Misc Feature(10)720..722(+)
Misc Feature(11)963..965(+)
Misc Feature(12)963..965(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..133
Gene:TNNT2
Gene Synonym:
Exon (3)134..144
Gene:TNNT2
Gene Synonym:
Exon (4)145..159
Gene:TNNT2
Gene Synonym:
Exon (5)160..189
Gene:TNNT2
Gene Synonym:
Exon (6)190..255
Gene:TNNT2
Gene Synonym:
Exon (7)256..291
Gene:TNNT2
Gene Synonym:
Exon (8)292..325
Gene:TNNT2
Gene Synonym:
Exon (9)326..386
Gene:TNNT2
Gene Synonym:
Exon (10)387..503
Gene:TNNT2
Gene Synonym:
Exon (11)504..581
Gene:TNNT2
Gene Synonym:
Exon (12)582..692
Gene:TNNT2
Gene Synonym:
Exon (13)693..802
Gene:TNNT2
Gene Synonym:
Exon (14)803..893
Gene:TNNT2
Gene Synonym:
Exon (15)894..934
Gene:TNNT2
Gene Synonym:
Exon (16)935..1182
Gene:TNNT2
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, c dbSNP:45590943
11 11 g, t dbSNP:770524611
20 20 c, t dbSNP:746521973
27 27 c, t dbSNP:772939790
45 45 a, g dbSNP:771906661
75 75 a, t dbSNP:747865894
82 82 a, g, t dbSNP:201831895
88 88 a, g dbSNP:730881093
90 90 a, g dbSNP:752977897
92 92 a, c dbSNP:776936911
96 96 c, t dbSNP:765584396
102 102 a, c dbSNP:139705141
113 113 g, t dbSNP:777128825
125 125 c, t dbSNP:148027842
126 126 a, g dbSNP:760247765
131 131 a, g dbSNP:727503516
132 132 a, g dbSNP:772890125
146 146 a, g dbSNP:754664870
148 148 c, g dbSNP:753645200
151 151 c, t dbSNP:535282031
155 155 g, t dbSNP:397516477
159 159 a, g dbSNP:772966842
163 163 a, g dbSNP:767533710
167 167 a, g dbSNP:761895785
169 169 a, c dbSNP:751573686
176 176 a, g dbSNP:370895664
182 182 c, t dbSNP:727503515
186 186 c, g dbSNP:572078239
188 188 c, t dbSNP:751728017
189 189 a, g, t dbSNP:377474357
202 202 a, c, g dbSNP:776406819
204 204 a, g dbSNP:770771962
205 205 a, c, t dbSNP:200754249
206 206 a, g dbSNP:200283086
213 213 a, g dbSNP:748078123
223 223 c, t dbSNP:778730615
227 227 a, g dbSNP:756174289
228 228 c, g dbSNP:397516447
236 236 c, t dbSNP:746492909
237 237 a, g dbSNP:757526942
255 255 a, g dbSNP:730881120
258 258 a, g dbSNP:377341875
260 260 c, t dbSNP:771688910
267 267 c, g dbSNP:568628521
269 269 -, aga dbSNP:781616719
272 272 g, t dbSNP:774064875
286 286 a, c dbSNP:768463992
299 299 a, g dbSNP:531954320
300 300 a, c, g dbSNP:141837529
305 305 a, g dbSNP:771980829
314 314 a, g dbSNP:781024179
316 316 a, c dbSNP:371394312
319 319 -, a dbSNP:34008819
320 320 a, c, g dbSNP:727504869
321 321 a, c dbSNP:774702133
322 322 c, t dbSNP:769040140
328 328 c, t dbSNP:761953142
329 329 a, g dbSNP:3729845
337 337 a, c dbSNP:386352374
340 340 a, g dbSNP:397516450
346 346 g, t dbSNP:730881095
347 347 g, t dbSNP:780115529
351 351 a, c dbSNP:730881096
352 352 c, t dbSNP:144900708
356 356 a, g dbSNP:769993507
357 357 a, t dbSNP:746297911
358 358 a, t dbSNP:121964855
360 360 a, c, t dbSNP:397516451
361 361 c, t dbSNP:730881121
362 362 c, g, t dbSNP:140245123
365 365 c, t dbSNP:763782935
366 366 a, g dbSNP:727504255
369 369 a, g dbSNP:727504244
371 371 c, g dbSNP:727503514
373 373 -, g dbSNP:780087395
373 373 c, g dbSNP:397516452
374 374 a, t dbSNP:397516453
379 379 a, c dbSNP:397516455
383 383 g, t dbSNP:730881098
386 386 g, t dbSNP:397516454
389 389 c, t dbSNP:771514445
395 395 c, t dbSNP:267598299
396 396 -, ttc dbSNP:730881117
396 396 c, t dbSNP:397516456
397 397 a, g dbSNP:121964856
399 399 a, c dbSNP:730881122
401 401 a, g dbSNP:778426227
402 402 c, t dbSNP:727503513
403 403 a, g, t dbSNP:397516457
405 405 a, g dbSNP:397516458
406 406 c, t dbSNP:748914885
407 407 -, gga dbSNP:727504277
413 413 g, t dbSNP:397516459
417 417 c, t dbSNP:779857935
421 421 a, g dbSNP:727505027
433 433 c, t dbSNP:727504245
440 440 c, g, t dbSNP:3729547
441 441 a, g, t dbSNP:730881099
447 447 c, t dbSNP:397516460
450 450 a, t dbSNP:121964858
452 452 g, t dbSNP:727504331
467 467 -, a dbSNP:560019679
473 473 a, g dbSNP:201898087
474 474 a, g dbSNP:397516461
479 479 a, g dbSNP:199992160
480 480 c, g dbSNP:767617578
482 482 c, g dbSNP:758543857
483 483 a, g dbSNP:537067344
495 495 a, g dbSNP:765359025
496 496 a, g dbSNP:759758840
498 498 a, t dbSNP:786204405
503 503 c, t dbSNP:727504322
504 504 a, g dbSNP:730881100
508 508 a, g dbSNP:2996496
510 510 c, t dbSNP:397516463
513 513 c, t dbSNP:74315380
514 514 a, c, g dbSNP:397516464
522 522 a, c, g, t dbSNP:45525839
523 523 a, g dbSNP:754037135
524 524 a, g dbSNP:112453991
527 527 c, t dbSNP:375675827
528 528 a, c, g dbSNP:371142225
530 530 g, t dbSNP:141754300
535 535 a, g dbSNP:730880232
537 537 c, t dbSNP:397516465
538 538 a, g dbSNP:397516466
540 540 a, g dbSNP:762340105
542 542 c, t dbSNP:200604266
543 543 a, c, t dbSNP:74315379
543 543 -, c dbSNP:730881115
544 544 a, g dbSNP:730881101
545 545 c, g dbSNP:786204406
547 547 a, g dbSNP:748804579
551 551 a, g dbSNP:775274966
552 552 c, t dbSNP:483352832
553 553 a, g dbSNP:745632066
564 564 c, t dbSNP:730881123
565 565 a, g dbSNP:730881102
566 566 c, g dbSNP:35914325
571 571 a, g dbSNP:770666870
572 572 a, c dbSNP:786205908
573 573 c, t dbSNP:45608937
574 574 a, g dbSNP:201048783
579 579 a, g dbSNP:727505030
589 589 a, g dbSNP:777763436
590 590 a, g dbSNP:750399630
594 594 c, g, t dbSNP:730881103
595 595 a, g dbSNP:397516468
597 597 c, t dbSNP:397516469
598 598 a, g dbSNP:45501500
608 608 gg, tt dbSNP:730881118
609 609 -, gag dbSNP:397516470
610 610 a, g dbSNP:780067626
614 614 a, c, t dbSNP:483352833
618 618 a, g dbSNP:730881104
636 636 g, t dbSNP:730881097
639 639 c, g dbSNP:727503512
640 640 a, g dbSNP:397516471
644 644 a, g dbSNP:566113559
645 645 a, c dbSNP:558549356
650 650 c, g dbSNP:757634539
652 652 a, c dbSNP:397516473
656 656 c, g dbSNP:201270895
657 657 g, t dbSNP:377157235
658 658 c, t dbSNP:727504246
659 659 c, t dbSNP:397516474
662 662 c, t dbSNP:764636823
668 668 a, g dbSNP:763316865
669 669 c, t dbSNP:753292088
675 675 g, t dbSNP:764777623
683 683 c, t dbSNP:759134258
684 684 a, g dbSNP:730881124
691 691 a, c dbSNP:730881105
693 693 a, g dbSNP:587782981
696 696 a, g dbSNP:150008205
697 697 -, ag dbSNP:760664767
699 699 c, t dbSNP:730881106
700 700 a, g dbSNP:371047521
711 711 a, g dbSNP:727503511
721 721 c, t dbSNP:397516476
725 725 a, g dbSNP:772300998
726 726 a, c, t dbSNP:45586240
727 727 a, g, t dbSNP:121964860
741 741 -, aag dbSNP:121964859
742 742 -, aga dbSNP:45578238
742 742 a, t dbSNP:190805300
748 748 a, t dbSNP:750800588
753 753 a, g dbSNP:769128006
762 762 a, g dbSNP:397516478
765 765 g, t dbSNP:397516479
775 775 c, t dbSNP:45520032
789 789 a, g dbSNP:730881107
795 795 c, g dbSNP:730881108
806 806 a, g dbSNP:201229194
813 813 a, g dbSNP:746592264
819 819 c, t dbSNP:786204407
829 829 c, g dbSNP:2996495
830 830 c, g dbSNP:3020555
835 835 a, g dbSNP:200500421
845 845 g, t dbSNP:45466197
847 847 c, t dbSNP:369181536
850 850 a, g dbSNP:730881109
855 855 c, g, t dbSNP:730881110
857 857 c, t dbSNP:397516481
858 858 a, g dbSNP:141805127
862 862 a, c, t dbSNP:376037051
864 864 a, c dbSNP:730881111
867 867 a, g dbSNP:727504488
871 871 a, g dbSNP:3730238
886 886 a, t dbSNP:397516482
888 888 c, t dbSNP:730881112
890 890 c, t dbSNP:757379649
895 895 c, t dbSNP:371384395
896 896 c, t dbSNP:761288511
898 898 a, g dbSNP:397516483
899 899 c, t dbSNP:768470426
906 906 a, c dbSNP:748970759
907 907 a, g dbSNP:730881125
908 908 a, c dbSNP:786204408
918 918 a, g, t dbSNP:4523540
920 920 a, c, t dbSNP:376923877
921 921 a, g, t dbSNP:121964861
927 927 -, c dbSNP:730881119
941 941 a, g dbSNP:483352836
944 944 a, c dbSNP:754211195
945 945 c, t dbSNP:121964857
946 946 a, c, g dbSNP:397516484
947 947 c, t dbSNP:45503195
948 948 a, g dbSNP:757664792
949 949 a, g dbSNP:727505233
953 953 a, g dbSNP:752111672
961 961 a, t dbSNP:730880233
965 965 c, t dbSNP:45465693
966 966 a, g dbSNP:147940106
969 969 c, t dbSNP:367785431
970 970 a, g, t dbSNP:141121678
973 973 a, g dbSNP:727504247
974 974 a, g dbSNP:730881116
976 976 a, g dbSNP:770794082
977 977 a, g dbSNP:746858820
983 983 a, c dbSNP:529731863
988 988 c, t dbSNP:768709734
989 989 a, c, t dbSNP:370503996
993 993 g, t dbSNP:756239287
1002 1002 a, g dbSNP:746131983
1006 1006 g, t dbSNP:375935487
1008 1008 c, g, t dbSNP:201271311
1010 1010 c, t dbSNP:752096322
1011 1011 -, c dbSNP:776968084
1013 1013 c, t dbSNP:764761207
1014 1014 a, g dbSNP:757948388
1017 1017 c, t dbSNP:149240770
1018 1018 a, c, g dbSNP:111735792
1019 1019 c, t dbSNP:553116893
1020 1020 a, c dbSNP:766030869
1029 1029 c, t dbSNP:546008385
1030 1030 a, g dbSNP:773131107
1046 1046 a, g dbSNP:3729998
1051 1051 c, t dbSNP:3730244
1103 1103 c, t dbSNP:185927345
1106 1106 c, g dbSNP:574804643
1130 1130 c, t dbSNP:554553266
1150 1150 c, g dbSNP:534887070

Target ORF information:

RefSeq Version NM_000364
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000364

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
ACAGAGCGGA AAAGTGGGAA GAGGCAGACT GAGCGGGAAA AGAAGAAGAA GATTCTGGCT
GAGAGGAGGA AGGTGCTGGC CATTGACCAC CTGAATGAAG ATCAGCTGAG GGAGAAGGCC
AAGGAGCTGT GGCAGAGCAT CTATAACTTG GAGGCAGAGA AGTTCGACCT GCAGGAGAAG
TTCAAGCAGC AGAAATATGA GATCAATGTT CTCCGAAACA GGATCAACGA TAACCAGAAA
GTCTCCAAGA CCCGCGGGAA GGCTAAAGTC ACCGGGCGCT GGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24695
Accession Version NM_001276347.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 867bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu24695D_COA.pdf (pdf)
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290621.1, BC002653.2 and AA865519.1. Summary: The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (7) differs in the 5' UTR and lacks an in-frame exon in the 5' coding region compared to variant 5. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 5. Variants 2 and 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290621.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG
AGGCAGACTG AGCGGGAAAA GAAGAAGAAG ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC
ATTGACCACC TGAATGAAGA TCAGCTGAGG GAGAAGGCCA AGGAGCTGTG GCAGAGCATC
TATAACTTGG AGGCAGAGAA GTTCGACCTG CAGGAGAAGT TCAAGCAGCA GAAATATGAG
ATCAATGTTC TCCGAAACAG GATCAACGAT AACCAGAAAG TCTCCAAGAC CCGCGGGAAG
GCTAAAGTCA CCGGGCGCTG GAAATAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001263276.1
CDS226..1092
Misc Feature(1)190..192(+)
Misc Feature(2)502..894(+)
Misc Feature(3)793..>1035(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..266
Gene:TNNT2
Gene Synonym:
Exon (3)267..277
Gene:TNNT2
Gene Synonym:
Exon (4)278..292
Gene:TNNT2
Gene Synonym:
Exon (5)293..358
Gene:TNNT2
Gene Synonym:
Exon (6)359..394
Gene:TNNT2
Gene Synonym:
Exon (7)395..428
Gene:TNNT2
Gene Synonym:
Exon (8)429..489
Gene:TNNT2
Gene Synonym:
Exon (9)490..606
Gene:TNNT2
Gene Synonym:
Exon (10)607..684
Gene:TNNT2
Gene Synonym:
Exon (11)685..795
Gene:TNNT2
Gene Synonym:
Exon (12)796..804
Gene:TNNT2
Gene Synonym:
Exon (13)805..914
Gene:TNNT2
Gene Synonym:
Exon (14)915..1005
Gene:TNNT2
Gene Synonym:
Exon (15)1006..1046
Gene:TNNT2
Gene Synonym:
Exon (16)1047..1294
Gene:TNNT2
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, c dbSNP:45590943
11 11 g, t dbSNP:770524611
20 20 c, t dbSNP:746521973
27 27 c, t dbSNP:772939790
45 45 a, g dbSNP:771906661
75 75 a, t dbSNP:747865894
95 95 a, g dbSNP:558913147
98 98 g, t dbSNP:539105911
114 114 a, c dbSNP:576423144
115 115 c, g dbSNP:761830318
120 120 c, t dbSNP:556637776
123 123 c, t dbSNP:536599596
132 132 a, c dbSNP:543810268
139 139 a, c dbSNP:567706341
140 140 c, t dbSNP:554107377
141 141 a, g dbSNP:763955491
161 161 a, g dbSNP:377579139
175 175 c, t dbSNP:762600119
181 181 c, t dbSNP:757273188
185 185 c, t dbSNP:751699004
199 199 c, t dbSNP:764292653
215 215 a, g, t dbSNP:201831895
221 221 a, g dbSNP:730881093
223 223 a, g dbSNP:752977897
225 225 a, c dbSNP:776936911
229 229 c, t dbSNP:765584396
235 235 a, c dbSNP:139705141
246 246 g, t dbSNP:777128825
258 258 c, t dbSNP:148027842
259 259 a, g dbSNP:760247765
264 264 a, g dbSNP:727503516
265 265 a, g dbSNP:772890125
279 279 a, g dbSNP:754664870
281 281 c, g dbSNP:753645200
284 284 c, t dbSNP:535282031
288 288 g, t dbSNP:397516477
292 292 a, g dbSNP:772966842
305 305 a, c, g dbSNP:776406819
307 307 a, g dbSNP:770771962
308 308 a, c, t dbSNP:200754249
309 309 a, g dbSNP:200283086
316 316 a, g dbSNP:748078123
326 326 c, t dbSNP:778730615
330 330 a, g dbSNP:756174289
331 331 c, g dbSNP:397516447
339 339 c, t dbSNP:746492909
340 340 a, g dbSNP:757526942
358 358 a, g dbSNP:730881120
361 361 a, g dbSNP:377341875
363 363 c, t dbSNP:771688910
370 370 c, g dbSNP:568628521
372 372 -, aga dbSNP:781616719
375 375 g, t dbSNP:774064875
389 389 a, c dbSNP:768463992
402 402 a, g dbSNP:531954320
403 403 a, c, g dbSNP:141837529
408 408 a, g dbSNP:771980829
417 417 a, g dbSNP:781024179
419 419 a, c dbSNP:371394312
422 422 -, a dbSNP:34008819
423 423 a, c, g dbSNP:727504869
424 424 a, c dbSNP:774702133
425 425 c, t dbSNP:769040140
431 431 c, t dbSNP:761953142
432 432 a, g dbSNP:3729845
440 440 a, c dbSNP:386352374
443 443 a, g dbSNP:397516450
449 449 g, t dbSNP:730881095
450 450 g, t dbSNP:780115529
454 454 a, c dbSNP:730881096
455 455 c, t dbSNP:144900708
459 459 a, g dbSNP:769993507
460 460 a, t dbSNP:746297911
461 461 a, t dbSNP:121964855
463 463 a, c, t dbSNP:397516451
464 464 c, t dbSNP:730881121
465 465 c, g, t dbSNP:140245123
468 468 c, t dbSNP:763782935
469 469 a, g dbSNP:727504255
472 472 a, g dbSNP:727504244
474 474 c, g dbSNP:727503514
476 476 -, g dbSNP:780087395
476 476 c, g dbSNP:397516452
477 477 a, t dbSNP:397516453
482 482 a, c dbSNP:397516455
486 486 g, t dbSNP:730881098
489 489 g, t dbSNP:397516454
492 492 c, t dbSNP:771514445
498 498 c, t dbSNP:267598299
499 499 -, ttc dbSNP:730881117
499 499 c, t dbSNP:397516456
500 500 a, g dbSNP:121964856
502 502 a, c dbSNP:730881122
504 504 a, g dbSNP:778426227
505 505 c, t dbSNP:727503513
506 506 a, g, t dbSNP:397516457
508 508 a, g dbSNP:397516458
509 509 c, t dbSNP:748914885
510 510 -, gga dbSNP:727504277
516 516 g, t dbSNP:397516459
520 520 c, t dbSNP:779857935
524 524 a, g dbSNP:727505027
536 536 c, t dbSNP:727504245
543 543 c, g, t dbSNP:3729547
544 544 a, g, t dbSNP:730881099
550 550 c, t dbSNP:397516460
553 553 a, t dbSNP:121964858
555 555 g, t dbSNP:727504331
570 570 -, a dbSNP:560019679
576 576 a, g dbSNP:201898087
577 577 a, g dbSNP:397516461
582 582 a, g dbSNP:199992160
583 583 c, g dbSNP:767617578
585 585 c, g dbSNP:758543857
586 586 a, g dbSNP:537067344
598 598 a, g dbSNP:765359025
599 599 a, g dbSNP:759758840
601 601 a, t dbSNP:786204405
606 606 c, t dbSNP:727504322
607 607 a, g dbSNP:730881100
611 611 a, g dbSNP:2996496
613 613 c, t dbSNP:397516463
616 616 c, t dbSNP:74315380
617 617 a, c, g dbSNP:397516464
625 625 a, c, g, t dbSNP:45525839
626 626 a, g dbSNP:754037135
627 627 a, g dbSNP:112453991
630 630 c, t dbSNP:375675827
631 631 a, c, g dbSNP:371142225
633 633 g, t dbSNP:141754300
638 638 a, g dbSNP:730880232
640 640 c, t dbSNP:397516465
641 641 a, g dbSNP:397516466
643 643 a, g dbSNP:762340105
645 645 c, t dbSNP:200604266
646 646 a, c, t dbSNP:74315379
646 646 -, c dbSNP:730881115
647 647 a, g dbSNP:730881101
648 648 c, g dbSNP:786204406
650 650 a, g dbSNP:748804579
654 654 a, g dbSNP:775274966
655 655 c, t dbSNP:483352832
656 656 a, g dbSNP:745632066
667 667 c, t dbSNP:730881123
668 668 a, g dbSNP:730881102
669 669 c, g dbSNP:35914325
674 674 a, g dbSNP:770666870
675 675 a, c dbSNP:786205908
676 676 c, t dbSNP:45608937
677 677 a, g dbSNP:201048783
682 682 a, g dbSNP:727505030
692 692 a, g dbSNP:777763436
693 693 a, g dbSNP:750399630
697 697 c, g, t dbSNP:730881103
698 698 a, g dbSNP:397516468
700 700 c, t dbSNP:397516469
701 701 a, g dbSNP:45501500
711 711 gg, tt dbSNP:730881118
712 712 -, gag dbSNP:397516470
713 713 a, g dbSNP:780067626
717 717 a, c, t dbSNP:483352833
721 721 a, g dbSNP:730881104
739 739 g, t dbSNP:730881097
742 742 c, g dbSNP:727503512
743 743 a, g dbSNP:397516471
747 747 a, g dbSNP:566113559
748 748 a, c dbSNP:558549356
753 753 c, g dbSNP:757634539
755 755 a, c dbSNP:397516473
759 759 c, g dbSNP:201270895
760 760 g, t dbSNP:377157235
761 761 c, t dbSNP:727504246
762 762 c, t dbSNP:397516474
765 765 c, t dbSNP:764636823
771 771 a, g dbSNP:763316865
772 772 c, t dbSNP:753292088
778 778 g, t dbSNP:764777623
786 786 c, t dbSNP:759134258
787 787 a, g dbSNP:730881124
794 794 a, c dbSNP:730881105
801 801 c, t dbSNP:767158813
805 805 a, g dbSNP:587782981
808 808 a, g dbSNP:150008205
809 809 -, ag dbSNP:760664767
811 811 c, t dbSNP:730881106
812 812 a, g dbSNP:371047521
823 823 a, g dbSNP:727503511
833 833 c, t dbSNP:397516476
837 837 a, g dbSNP:772300998
838 838 a, c, t dbSNP:45586240
839 839 a, g, t dbSNP:121964860
853 853 -, aag dbSNP:121964859
854 854 -, aga dbSNP:45578238
854 854 a, t dbSNP:190805300
860 860 a, t dbSNP:750800588
865 865 a, g dbSNP:769128006
874 874 a, g dbSNP:397516478
877 877 g, t dbSNP:397516479
887 887 c, t dbSNP:45520032
901 901 a, g dbSNP:730881107
907 907 c, g dbSNP:730881108
918 918 a, g dbSNP:201229194
925 925 a, g dbSNP:746592264
931 931 c, t dbSNP:786204407
941 941 c, g dbSNP:2996495
942 942 c, g dbSNP:3020555
947 947 a, g dbSNP:200500421
957 957 g, t dbSNP:45466197
959 959 c, t dbSNP:369181536
962 962 a, g dbSNP:730881109
967 967 c, g, t dbSNP:730881110
969 969 c, t dbSNP:397516481
970 970 a, g dbSNP:141805127
974 974 a, c, t dbSNP:376037051
976 976 a, c dbSNP:730881111
979 979 a, g dbSNP:727504488
983 983 a, g dbSNP:3730238
998 998 a, t dbSNP:397516482
1000 1000 c, t dbSNP:730881112
1002 1002 c, t dbSNP:757379649
1007 1007 c, t dbSNP:371384395
1008 1008 c, t dbSNP:761288511
1010 1010 a, g dbSNP:397516483
1011 1011 c, t dbSNP:768470426
1018 1018 a, c dbSNP:748970759
1019 1019 a, g dbSNP:730881125
1020 1020 a, c dbSNP:786204408
1030 1030 a, g, t dbSNP:4523540
1032 1032 a, c, t dbSNP:376923877
1033 1033 a, g, t dbSNP:121964861
1039 1039 -, c dbSNP:730881119
1053 1053 a, g dbSNP:483352836
1056 1056 a, c dbSNP:754211195
1057 1057 c, t dbSNP:121964857
1058 1058 a, c, g dbSNP:397516484
1059 1059 c, t dbSNP:45503195
1060 1060 a, g dbSNP:757664792
1061 1061 a, g dbSNP:727505233
1065 1065 a, g dbSNP:752111672
1073 1073 a, t dbSNP:730880233
1077 1077 c, t dbSNP:45465693
1078 1078 a, g dbSNP:147940106
1081 1081 c, t dbSNP:367785431
1082 1082 a, g, t dbSNP:141121678
1085 1085 a, g dbSNP:727504247
1086 1086 a, g dbSNP:730881116
1088 1088 a, g dbSNP:770794082
1089 1089 a, g dbSNP:746858820
1095 1095 a, c dbSNP:529731863
1100 1100 c, t dbSNP:768709734
1101 1101 a, c, t dbSNP:370503996
1105 1105 g, t dbSNP:756239287
1114 1114 a, g dbSNP:746131983
1118 1118 g, t dbSNP:375935487
1120 1120 c, g, t dbSNP:201271311
1122 1122 c, t dbSNP:752096322
1123 1123 -, c dbSNP:776968084
1125 1125 c, t dbSNP:764761207
1126 1126 a, g dbSNP:757948388
1129 1129 c, t dbSNP:149240770
1130 1130 a, c, g dbSNP:111735792
1131 1131 c, t dbSNP:553116893
1132 1132 a, c dbSNP:766030869
1141 1141 c, t dbSNP:546008385
1142 1142 a, g dbSNP:773131107
1158 1158 a, g dbSNP:3729998
1163 1163 c, t dbSNP:3730244
1215 1215 c, t dbSNP:185927345
1218 1218 c, g dbSNP:574804643
1242 1242 c, t dbSNP:554553266
1262 1262 c, g dbSNP:534887070

Target ORF information:

RefSeq Version NM_001276347
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276347

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG
AGGCAGACTG AGCGGGAAAA GAAGAAGAAG ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC
ATTGACCACC TGAATGAAGA TCAGCTGAGG GAGAAGGCCA AGGAGCTGTG GCAGAGCATC
TATAACTTGG AGGCAGAGAA GTTCGACCTG CAGGAGAAGT TCAAGCAGCA GAAATATGAG
ATCAATGTTC TCCGAAACAG GATCAACGAT AACCAGAAAG TCTCCAAGAC CCGCGGGAAG
GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25008
Accession Version NM_001276346.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 768bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ709280.1, X79857.1 and AA865519.1. Summary: The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (6) lacks multiple in-frame exons and uses an alternate in-frame splice site in the 5' coding region, compared to variant 5. It encodes a shorter isoform (6) compared to isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGATGAAGA AGAAGAGGAA
GCAAAGGAGG CTGAAGATGG CCCAATGGAG GAGTCCAAAC CAAAGCCCAG GTCGTTCATG
CCCAACTTGG TGCCTCCCAA GATCCCCGAT GGAGAGAGAG TGGACTTTGA TGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
ACAGAGCGGA AAAGTGGGAA GAGGCAGACT GAGCGGGAAA AGAAGAAGAA GATTCTGGCT
GAGAGGAGGA AGGTGCTGGC CATTGACCAC CTGAATGAAG ATCAGCTGAG GGAGAAGGCC
AAGGAGCTGT GGCAGAGCAT CTATAACTTG GAGGCAGAGA AGTTCGACCT GCAGGAGAAG
TTCAAGCAGC AGAAATATGA GATCAATGTT CTCCGAAACA GGATCAACGA TAACCAGAAA
GTCTCCAAGA CCCGCGGGAA GGCTAAAGTC ACCGGGCGCT GGAAATAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001263275.1
CDS93..860
Misc Feature(1)75..77(+)
Misc Feature(2)603..>803(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..133
Gene:TNNT2
Gene Synonym:
Exon (3)134..144
Gene:TNNT2
Gene Synonym:
Exon (4)145..159
Gene:TNNT2
Gene Synonym:
Exon (5)160..189
Gene:TNNT2
Gene Synonym:
Exon (6)190..255
Gene:TNNT2
Gene Synonym:
Exon (7)256..288
Gene:TNNT2
Gene Synonym:
Exon (8)289..322
Gene:TNNT2
Gene Synonym:
Exon (9)323..383
Gene:TNNT2
Gene Synonym:
Exon (10)384..461
Gene:TNNT2
Gene Synonym:
Exon (11)462..572
Gene:TNNT2
Gene Synonym:
Exon (12)573..682
Gene:TNNT2
Gene Synonym:
Exon (13)683..773
Gene:TNNT2
Gene Synonym:
Exon (14)774..814
Gene:TNNT2
Gene Synonym:
Exon (15)815..1062
Gene:TNNT2
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, c dbSNP:45590943
11 11 g, t dbSNP:770524611
20 20 c, t dbSNP:746521973
27 27 c, t dbSNP:772939790
45 45 a, g dbSNP:771906661
75 75 a, t dbSNP:747865894
82 82 a, g, t dbSNP:201831895
88 88 a, g dbSNP:730881093
90 90 a, g dbSNP:752977897
92 92 a, c dbSNP:776936911
96 96 c, t dbSNP:765584396
102 102 a, c dbSNP:139705141
113 113 g, t dbSNP:777128825
125 125 c, t dbSNP:148027842
126 126 a, g dbSNP:760247765
131 131 a, g dbSNP:727503516
132 132 a, g dbSNP:772890125
146 146 a, g dbSNP:754664870
148 148 c, g dbSNP:753645200
151 151 c, t dbSNP:535282031
155 155 g, t dbSNP:397516477
159 159 a, g dbSNP:772966842
163 163 a, g dbSNP:767533710
167 167 a, g dbSNP:761895785
169 169 a, c dbSNP:751573686
176 176 a, g dbSNP:370895664
182 182 c, t dbSNP:727503515
186 186 c, g dbSNP:572078239
188 188 c, t dbSNP:751728017
189 189 a, g, t dbSNP:377474357
202 202 a, c, g dbSNP:776406819
204 204 a, g dbSNP:770771962
205 205 a, c, t dbSNP:200754249
206 206 a, g dbSNP:200283086
213 213 a, g dbSNP:748078123
223 223 c, t dbSNP:778730615
227 227 a, g dbSNP:756174289
228 228 c, g dbSNP:397516447
236 236 c, t dbSNP:746492909
237 237 a, g dbSNP:757526942
255 255 a, g dbSNP:730881120
257 257 c, t dbSNP:771688910
264 264 c, g dbSNP:568628521
266 266 -, aga dbSNP:781616719
269 269 g, t dbSNP:774064875
283 283 a, c dbSNP:768463992
296 296 a, g dbSNP:531954320
297 297 a, c, g dbSNP:141837529
302 302 a, g dbSNP:771980829
311 311 a, g dbSNP:781024179
313 313 a, c dbSNP:371394312
316 316 -, a dbSNP:34008819
317 317 a, c, g dbSNP:727504869
318 318 a, c dbSNP:774702133
319 319 c, t dbSNP:769040140
325 325 c, t dbSNP:761953142
326 326 a, g dbSNP:3729845
334 334 a, c dbSNP:386352374
337 337 a, g dbSNP:397516450
343 343 g, t dbSNP:730881095
344 344 g, t dbSNP:780115529
348 348 a, c dbSNP:730881096
349 349 c, t dbSNP:144900708
353 353 a, g dbSNP:769993507
354 354 a, t dbSNP:746297911
355 355 a, t dbSNP:121964855
357 357 a, c, t dbSNP:397516451
358 358 c, t dbSNP:730881121
359 359 c, g, t dbSNP:140245123
362 362 c, t dbSNP:763782935
363 363 a, g dbSNP:727504255
366 366 a, g dbSNP:727504244
368 368 c, g dbSNP:727503514
370 370 -, g dbSNP:780087395
370 370 c, g dbSNP:397516452
371 371 a, t dbSNP:397516453
376 376 a, c dbSNP:397516455
380 380 g, t dbSNP:730881098
383 383 g, t dbSNP:397516454
384 384 a, g dbSNP:730881100
388 388 a, g dbSNP:2996496
390 390 c, t dbSNP:397516463
393 393 c, t dbSNP:74315380
394 394 a, c, g dbSNP:397516464
402 402 a, c, g, t dbSNP:45525839
403 403 a, g dbSNP:754037135
404 404 a, g dbSNP:112453991
407 407 c, t dbSNP:375675827
408 408 a, c, g dbSNP:371142225
410 410 g, t dbSNP:141754300
415 415 a, g dbSNP:730880232
417 417 c, t dbSNP:397516465
418 418 a, g dbSNP:397516466
420 420 a, g dbSNP:762340105
422 422 c, t dbSNP:200604266
423 423 a, c, t dbSNP:74315379
423 423 -, c dbSNP:730881115
424 424 a, g dbSNP:730881101
425 425 c, g dbSNP:786204406
427 427 a, g dbSNP:748804579
431 431 a, g dbSNP:775274966
432 432 c, t dbSNP:483352832
433 433 a, g dbSNP:745632066
444 444 c, t dbSNP:730881123
445 445 a, g dbSNP:730881102
446 446 c, g dbSNP:35914325
451 451 a, g dbSNP:770666870
452 452 a, c dbSNP:786205908
453 453 c, t dbSNP:45608937
454 454 a, g dbSNP:201048783
459 459 a, g dbSNP:727505030
469 469 a, g dbSNP:777763436
470 470 a, g dbSNP:750399630
474 474 c, g, t dbSNP:730881103
475 475 a, g dbSNP:397516468
477 477 c, t dbSNP:397516469
478 478 a, g dbSNP:45501500
488 488 gg, tt dbSNP:730881118
489 489 -, gag dbSNP:397516470
490 490 a, g dbSNP:780067626
494 494 a, c, t dbSNP:483352833
498 498 a, g dbSNP:730881104
516 516 g, t dbSNP:730881097
519 519 c, g dbSNP:727503512
520 520 a, g dbSNP:397516471
524 524 a, g dbSNP:566113559
525 525 a, c dbSNP:558549356
530 530 c, g dbSNP:757634539
532 532 a, c dbSNP:397516473
536 536 c, g dbSNP:201270895
537 537 g, t dbSNP:377157235
538 538 c, t dbSNP:727504246
539 539 c, t dbSNP:397516474
542 542 c, t dbSNP:764636823
548 548 a, g dbSNP:763316865
549 549 c, t dbSNP:753292088
555 555 g, t dbSNP:764777623
563 563 c, t dbSNP:759134258
564 564 a, g dbSNP:730881124
571 571 a, c dbSNP:730881105
573 573 a, g dbSNP:587782981
576 576 a, g dbSNP:150008205
577 577 -, ag dbSNP:760664767
579 579 c, t dbSNP:730881106
580 580 a, g dbSNP:371047521
591 591 a, g dbSNP:727503511
601 601 c, t dbSNP:397516476
605 605 a, g dbSNP:772300998
606 606 a, c, t dbSNP:45586240
607 607 a, g, t dbSNP:121964860
621 621 -, aag dbSNP:121964859
622 622 -, aga dbSNP:45578238
622 622 a, t dbSNP:190805300
628 628 a, t dbSNP:750800588
633 633 a, g dbSNP:769128006
642 642 a, g dbSNP:397516478
645 645 g, t dbSNP:397516479
655 655 c, t dbSNP:45520032
669 669 a, g dbSNP:730881107
675 675 c, g dbSNP:730881108
686 686 a, g dbSNP:201229194
693 693 a, g dbSNP:746592264
699 699 c, t dbSNP:786204407
709 709 c, g dbSNP:2996495
710 710 c, g dbSNP:3020555
715 715 a, g dbSNP:200500421
725 725 g, t dbSNP:45466197
727 727 c, t dbSNP:369181536
730 730 a, g dbSNP:730881109
735 735 c, g, t dbSNP:730881110
737 737 c, t dbSNP:397516481
738 738 a, g dbSNP:141805127
742 742 a, c, t dbSNP:376037051
744 744 a, c dbSNP:730881111
747 747 a, g dbSNP:727504488
751 751 a, g dbSNP:3730238
766 766 a, t dbSNP:397516482
768 768 c, t dbSNP:730881112
770 770 c, t dbSNP:757379649
775 775 c, t dbSNP:371384395
776 776 c, t dbSNP:761288511
778 778 a, g dbSNP:397516483
779 779 c, t dbSNP:768470426
786 786 a, c dbSNP:748970759
787 787 a, g dbSNP:730881125
788 788 a, c dbSNP:786204408
798 798 a, g, t dbSNP:4523540
800 800 a, c, t dbSNP:376923877
801 801 a, g, t dbSNP:121964861
807 807 -, c dbSNP:730881119
821 821 a, g dbSNP:483352836
824 824 a, c dbSNP:754211195
825 825 c, t dbSNP:121964857
826 826 a, c, g dbSNP:397516484
827 827 c, t dbSNP:45503195
828 828 a, g dbSNP:757664792
829 829 a, g dbSNP:727505233
833 833 a, g dbSNP:752111672
841 841 a, t dbSNP:730880233
845 845 c, t dbSNP:45465693
846 846 a, g dbSNP:147940106
849 849 c, t dbSNP:367785431
850 850 a, g, t dbSNP:141121678
853 853 a, g dbSNP:727504247
854 854 a, g dbSNP:730881116
856 856 a, g dbSNP:770794082
857 857 a, g dbSNP:746858820
863 863 a, c dbSNP:529731863
868 868 c, t dbSNP:768709734
869 869 a, c, t dbSNP:370503996
873 873 g, t dbSNP:756239287
882 882 a, g dbSNP:746131983
886 886 g, t dbSNP:375935487
888 888 c, g, t dbSNP:201271311
890 890 c, t dbSNP:752096322
891 891 -, c dbSNP:776968084
893 893 c, t dbSNP:764761207
894 894 a, g dbSNP:757948388
897 897 c, t dbSNP:149240770
898 898 a, c, g dbSNP:111735792
899 899 c, t dbSNP:553116893
900 900 a, c dbSNP:766030869
909 909 c, t dbSNP:546008385
910 910 a, g dbSNP:773131107
926 926 a, g dbSNP:3729998
931 931 c, t dbSNP:3730244
983 983 c, t dbSNP:185927345
986 986 c, g dbSNP:574804643
1010 1010 c, t dbSNP:554553266
1030 1030 c, g dbSNP:534887070

Target ORF information:

RefSeq Version NM_001276346
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276346

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGATGAAGA AGAAGAGGAA
GCAAAGGAGG CTGAAGATGG CCCAATGGAG GAGTCCAAAC CAAAGCCCAG GTCGTTCATG
CCCAACTTGG TGCCTCCCAA GATCCCCGAT GGAGAGAGAG TGGACTTTGA TGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
ACAGAGCGGA AAAGTGGGAA GAGGCAGACT GAGCGGGAAA AGAAGAAGAA GATTCTGGCT
GAGAGGAGGA AGGTGCTGGC CATTGACCAC CTGAATGAAG ATCAGCTGAG GGAGAAGGCC
AAGGAGCTGT GGCAGAGCAT CTATAACTTG GAGGCAGAGA AGTTCGACCT GCAGGAGAAG
TTCAAGCAGC AGAAATATGA GATCAATGTT CTCCGAAACA GGATCAACGA TAACCAGAAA
GTCTCCAAGA CCCGCGGGAA GGCTAAAGTC ACCGGGCGCT GGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25054
Accession Version NM_001276345.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 897bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ709280.1, X79855.1, BC002653.2, L40162.1 and AA865519.1. Summary: The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (5) encodes the longest isoform (5). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 12093807, 9689598 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG AGGCAGACTG AGCGGGAAAA GAAGAAGAAG
ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC ATTGACCACC TGAATGAAGA TCAGCTGAGG
GAGAAGGCCA AGGAGCTGTG GCAGAGCATC TATAACTTGG AGGCAGAGAA GTTCGACCTG
CAGGAGAAGT TCAAGCAGCA GAAATATGAG ATCAATGTTC TCCGAAACAG GATCAACGAT
AACCAGAAAG TCTCCAAGAC CCGCGGGAAG GCTAAAGTCA CCGGGCGCTG GAAATAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001263274.1
CDS93..989
Misc Feature(1)75..77(+)
Misc Feature(2)96..98(+)
Misc Feature(3)96..98(+)
Misc Feature(4)399..791(+)
Misc Feature(5)702..704(+)
Misc Feature(6)714..716(+)
Misc Feature(7)729..731(+)
Misc Feature(8)972..974(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..133
Gene:TNNT2
Gene Synonym:
Exon (3)134..144
Gene:TNNT2
Gene Synonym:
Exon (4)145..159
Gene:TNNT2
Gene Synonym:
Exon (5)160..189
Gene:TNNT2
Gene Synonym:
Exon (6)190..255
Gene:TNNT2
Gene Synonym:
Exon (7)256..291
Gene:TNNT2
Gene Synonym:
Exon (8)292..325
Gene:TNNT2
Gene Synonym:
Exon (9)326..386
Gene:TNNT2
Gene Synonym:
Exon (10)387..503
Gene:TNNT2
Gene Synonym:
Exon (11)504..581
Gene:TNNT2
Gene Synonym:
Exon (12)582..692
Gene:TNNT2
Gene Synonym:
Exon (13)693..701
Gene:TNNT2
Gene Synonym:
Exon (14)702..811
Gene:TNNT2
Gene Synonym:
Exon (15)812..902
Gene:TNNT2
Gene Synonym:
Exon (16)903..943
Gene:TNNT2
Gene Synonym:
Exon (17)944..1191
Gene:TNNT2
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, c dbSNP:45590943
11 11 g, t dbSNP:770524611
20 20 c, t dbSNP:746521973
27 27 c, t dbSNP:772939790
45 45 a, g dbSNP:771906661
75 75 a, t dbSNP:747865894
82 82 a, g, t dbSNP:201831895
88 88 a, g dbSNP:730881093
90 90 a, g dbSNP:752977897
92 92 a, c dbSNP:776936911
96 96 c, t dbSNP:765584396
102 102 a, c dbSNP:139705141
113 113 g, t dbSNP:777128825
125 125 c, t dbSNP:148027842
126 126 a, g dbSNP:760247765
131 131 a, g dbSNP:727503516
132 132 a, g dbSNP:772890125
146 146 a, g dbSNP:754664870
148 148 c, g dbSNP:753645200
151 151 c, t dbSNP:535282031
155 155 g, t dbSNP:397516477
159 159 a, g dbSNP:772966842
163 163 a, g dbSNP:767533710
167 167 a, g dbSNP:761895785
169 169 a, c dbSNP:751573686
176 176 a, g dbSNP:370895664
182 182 c, t dbSNP:727503515
186 186 c, g dbSNP:572078239
188 188 c, t dbSNP:751728017
189 189 a, g, t dbSNP:377474357
202 202 a, c, g dbSNP:776406819
204 204 a, g dbSNP:770771962
205 205 a, c, t dbSNP:200754249
206 206 a, g dbSNP:200283086
213 213 a, g dbSNP:748078123
223 223 c, t dbSNP:778730615
227 227 a, g dbSNP:756174289
228 228 c, g dbSNP:397516447
236 236 c, t dbSNP:746492909
237 237 a, g dbSNP:757526942
255 255 a, g dbSNP:730881120
258 258 a, g dbSNP:377341875
260 260 c, t dbSNP:771688910
267 267 c, g dbSNP:568628521
269 269 -, aga dbSNP:781616719
272 272 g, t dbSNP:774064875
286 286 a, c dbSNP:768463992
299 299 a, g dbSNP:531954320
300 300 a, c, g dbSNP:141837529
305 305 a, g dbSNP:771980829
314 314 a, g dbSNP:781024179
316 316 a, c dbSNP:371394312
319 319 -, a dbSNP:34008819
320 320 a, c, g dbSNP:727504869
321 321 a, c dbSNP:774702133
322 322 c, t dbSNP:769040140
328 328 c, t dbSNP:761953142
329 329 a, g dbSNP:3729845
337 337 a, c dbSNP:386352374
340 340 a, g dbSNP:397516450
346 346 g, t dbSNP:730881095
347 347 g, t dbSNP:780115529
351 351 a, c dbSNP:730881096
352 352 c, t dbSNP:144900708
356 356 a, g dbSNP:769993507
357 357 a, t dbSNP:746297911
358 358 a, t dbSNP:121964855
360 360 a, c, t dbSNP:397516451
361 361 c, t dbSNP:730881121
362 362 c, g, t dbSNP:140245123
365 365 c, t dbSNP:763782935
366 366 a, g dbSNP:727504255
369 369 a, g dbSNP:727504244
371 371 c, g dbSNP:727503514
373 373 -, g dbSNP:780087395
373 373 c, g dbSNP:397516452
374 374 a, t dbSNP:397516453
379 379 a, c dbSNP:397516455
383 383 g, t dbSNP:730881098
386 386 g, t dbSNP:397516454
389 389 c, t dbSNP:771514445
395 395 c, t dbSNP:267598299
396 396 -, ttc dbSNP:730881117
396 396 c, t dbSNP:397516456
397 397 a, g dbSNP:121964856
399 399 a, c dbSNP:730881122
401 401 a, g dbSNP:778426227
402 402 c, t dbSNP:727503513
403 403 a, g, t dbSNP:397516457
405 405 a, g dbSNP:397516458
406 406 c, t dbSNP:748914885
407 407 -, gga dbSNP:727504277
413 413 g, t dbSNP:397516459
417 417 c, t dbSNP:779857935
421 421 a, g dbSNP:727505027
433 433 c, t dbSNP:727504245
440 440 c, g, t dbSNP:3729547
441 441 a, g, t dbSNP:730881099
447 447 c, t dbSNP:397516460
450 450 a, t dbSNP:121964858
452 452 g, t dbSNP:727504331
467 467 -, a dbSNP:560019679
473 473 a, g dbSNP:201898087
474 474 a, g dbSNP:397516461
479 479 a, g dbSNP:199992160
480 480 c, g dbSNP:767617578
482 482 c, g dbSNP:758543857
483 483 a, g dbSNP:537067344
495 495 a, g dbSNP:765359025
496 496 a, g dbSNP:759758840
498 498 a, t dbSNP:786204405
503 503 c, t dbSNP:727504322
504 504 a, g dbSNP:730881100
508 508 a, g dbSNP:2996496
510 510 c, t dbSNP:397516463
513 513 c, t dbSNP:74315380
514 514 a, c, g dbSNP:397516464
522 522 a, c, g, t dbSNP:45525839
523 523 a, g dbSNP:754037135
524 524 a, g dbSNP:112453991
527 527 c, t dbSNP:375675827
528 528 a, c, g dbSNP:371142225
530 530 g, t dbSNP:141754300
535 535 a, g dbSNP:730880232
537 537 c, t dbSNP:397516465
538 538 a, g dbSNP:397516466
540 540 a, g dbSNP:762340105
542 542 c, t dbSNP:200604266
543 543 a, c, t dbSNP:74315379
543 543 -, c dbSNP:730881115
544 544 a, g dbSNP:730881101
545 545 c, g dbSNP:786204406
547 547 a, g dbSNP:748804579
551 551 a, g dbSNP:775274966
552 552 c, t dbSNP:483352832
553 553 a, g dbSNP:745632066
564 564 c, t dbSNP:730881123
565 565 a, g dbSNP:730881102
566 566 c, g dbSNP:35914325
571 571 a, g dbSNP:770666870
572 572 a, c dbSNP:786205908
573 573 c, t dbSNP:45608937
574 574 a, g dbSNP:201048783
579 579 a, g dbSNP:727505030
589 589 a, g dbSNP:777763436
590 590 a, g dbSNP:750399630
594 594 c, g, t dbSNP:730881103
595 595 a, g dbSNP:397516468
597 597 c, t dbSNP:397516469
598 598 a, g dbSNP:45501500
608 608 gg, tt dbSNP:730881118
609 609 -, gag dbSNP:397516470
610 610 a, g dbSNP:780067626
614 614 a, c, t dbSNP:483352833
618 618 a, g dbSNP:730881104
636 636 g, t dbSNP:730881097
639 639 c, g dbSNP:727503512
640 640 a, g dbSNP:397516471
644 644 a, g dbSNP:566113559
645 645 a, c dbSNP:558549356
650 650 c, g dbSNP:757634539
652 652 a, c dbSNP:397516473
656 656 c, g dbSNP:201270895
657 657 g, t dbSNP:377157235
658 658 c, t dbSNP:727504246
659 659 c, t dbSNP:397516474
662 662 c, t dbSNP:764636823
668 668 a, g dbSNP:763316865
669 669 c, t dbSNP:753292088
675 675 g, t dbSNP:764777623
683 683 c, t dbSNP:759134258
684 684 a, g dbSNP:730881124
691 691 a, c dbSNP:730881105
698 698 c, t dbSNP:767158813
702 702 a, g dbSNP:587782981
705 705 a, g dbSNP:150008205
706 706 -, ag dbSNP:760664767
708 708 c, t dbSNP:730881106
709 709 a, g dbSNP:371047521
720 720 a, g dbSNP:727503511
730 730 c, t dbSNP:397516476
734 734 a, g dbSNP:772300998
735 735 a, c, t dbSNP:45586240
736 736 a, g, t dbSNP:121964860
750 750 -, aag dbSNP:121964859
751 751 -, aga dbSNP:45578238
751 751 a, t dbSNP:190805300
757 757 a, t dbSNP:750800588
762 762 a, g dbSNP:769128006
771 771 a, g dbSNP:397516478
774 774 g, t dbSNP:397516479
784 784 c, t dbSNP:45520032
798 798 a, g dbSNP:730881107
804 804 c, g dbSNP:730881108
815 815 a, g dbSNP:201229194
822 822 a, g dbSNP:746592264
828 828 c, t dbSNP:786204407
838 838 c, g dbSNP:2996495
839 839 c, g dbSNP:3020555
844 844 a, g dbSNP:200500421
854 854 g, t dbSNP:45466197
856 856 c, t dbSNP:369181536
859 859 a, g dbSNP:730881109
864 864 c, g, t dbSNP:730881110
866 866 c, t dbSNP:397516481
867 867 a, g dbSNP:141805127
871 871 a, c, t dbSNP:376037051
873 873 a, c dbSNP:730881111
876 876 a, g dbSNP:727504488
880 880 a, g dbSNP:3730238
895 895 a, t dbSNP:397516482
897 897 c, t dbSNP:730881112
899 899 c, t dbSNP:757379649
904 904 c, t dbSNP:371384395
905 905 c, t dbSNP:761288511
907 907 a, g dbSNP:397516483
908 908 c, t dbSNP:768470426
915 915 a, c dbSNP:748970759
916 916 a, g dbSNP:730881125
917 917 a, c dbSNP:786204408
927 927 a, g, t dbSNP:4523540
929 929 a, c, t dbSNP:376923877
930 930 a, g, t dbSNP:121964861
936 936 -, c dbSNP:730881119
950 950 a, g dbSNP:483352836
953 953 a, c dbSNP:754211195
954 954 c, t dbSNP:121964857
955 955 a, c, g dbSNP:397516484
956 956 c, t dbSNP:45503195
957 957 a, g dbSNP:757664792
958 958 a, g dbSNP:727505233
962 962 a, g dbSNP:752111672
970 970 a, t dbSNP:730880233
974 974 c, t dbSNP:45465693
975 975 a, g dbSNP:147940106
978 978 c, t dbSNP:367785431
979 979 a, g, t dbSNP:141121678
982 982 a, g dbSNP:727504247
983 983 a, g dbSNP:730881116
985 985 a, g dbSNP:770794082
986 986 a, g dbSNP:746858820
992 992 a, c dbSNP:529731863
997 997 c, t dbSNP:768709734
998 998 a, c, t dbSNP:370503996
1002 1002 g, t dbSNP:756239287
1011 1011 a, g dbSNP:746131983
1015 1015 g, t dbSNP:375935487
1017 1017 c, g, t dbSNP:201271311
1019 1019 c, t dbSNP:752096322
1020 1020 -, c dbSNP:776968084
1022 1022 c, t dbSNP:764761207
1023 1023 a, g dbSNP:757948388
1026 1026 c, t dbSNP:149240770
1027 1027 a, c, g dbSNP:111735792
1028 1028 c, t dbSNP:553116893
1029 1029 a, c dbSNP:766030869
1038 1038 c, t dbSNP:546008385
1039 1039 a, g dbSNP:773131107
1055 1055 a, g dbSNP:3729998
1060 1060 c, t dbSNP:3730244
1112 1112 c, t dbSNP:185927345
1115 1115 c, g dbSNP:574804643
1139 1139 c, t dbSNP:554553266
1159 1159 c, g dbSNP:534887070

Target ORF information:

RefSeq Version NM_001276345
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276345

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG AGGCAGACTG AGCGGGAAAA GAAGAAGAAG
ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC ATTGACCACC TGAATGAAGA TCAGCTGAGG
GAGAAGGCCA AGGAGCTGTG GCAGAGCATC TATAACTTGG AGGCAGAGAA GTTCGACCTG
CAGGAGAAGT TCAAGCAGCA GAAATATGAG ATCAATGTTC TCCGAAACAG GATCAACGAT
AACCAGAAAG TCTCCAAGAC CCGCGGGAAG GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24894
Accession Version NM_001001432.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 849bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ709280.1, X79861.1, BC002653.2 and AA865519.1. On Feb 5, 2013 this sequence version replaced gi:48255882. Summary: The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (4) lacks multiple in-frame exons, compared to variant 5. It encodes a shorter isoform (4) compared to isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAGCAGGAG 
GAGGCAGCGG AAGAGGATGC TGAAGCAGAG GCTGAGACCG AGGAGACCAG GGCAGAAGAA
GATGAAGAAG AAGAGGAAGC AAAGGAGGCT GAAGATGGCC CAATGGAGGA GTCCAAACCA
AAGCCCAGGT CGTTCATGCC CAACTTGGTG CCTCCCAAGA TCCCCGATGG AGAGAGAGTG
GACTTTGATG ACATCCACCG GAAGCGCATG GAGAAGGACC TGAATGAGTT GCAGGCGCTG
ATCGAGGCTC ACTTTGAGAA CAGGAAGAAA GAGGAGGAGG AGCTCGTTTC TCTCAAAGAC
AGGATCGAGA GACGTCGGGC AGAGCGGGCC GAGCAGCAGC GCATCCGGAA TGAGCGGGAG
AAGGAGCGGC AGAACCGCCT GGCTGAAGAG AGGGCTCGAC GAGAGGAGGA GGAGAACAGG
AGGAAGGCTG AGGATGAGGC CCGGAAGAAG AAGGCTTTGT CCAACATGAT GCATTTTGGG
GGTTACATCC AGAAGGCCCA GACAGAGCGG AAAAGTGGGA AGAGGCAGAC TGAGCGGGAA
AAGAAGAAGA AGATTCTGGC TGAGAGGAGG AAGGTGCTGG CCATTGACCA CCTGAATGAA
GATCAGCTGA GGGAGAAGGC CAAGGAGCTG TGGCAGAGCA TCTATAACTT GGAGGCAGAG
AAGTTCGACC TGCAGGAGAA GTTCAAGCAG CAGAAATATG AGATCAATGT TCTCCGAAAC
AGGATCAACG ATAACCAGAA AGTCTCCAAG ACCCGCGGGA AGGCTAAAGT CACCGGGCGC
TGGAAATAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001001432.1
CDS93..941
Misc Feature(1)75..77(+)
Misc Feature(2)354..743(+)
Misc Feature(3)642..>884(+)
Misc Feature(4)654..656(+)
Misc Feature(5)666..668(+)
Misc Feature(6)681..683(+)
Misc Feature(7)924..926(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..133
Gene:TNNT2
Gene Synonym:
Exon (3)134..144
Gene:TNNT2
Gene Synonym:
Exon (4)145..210
Gene:TNNT2
Gene Synonym:
Exon (5)211..246
Gene:TNNT2
Gene Synonym:
Exon (6)247..280
Gene:TNNT2
Gene Synonym:
Exon (7)281..341
Gene:TNNT2
Gene Synonym:
Exon (8)342..458
Gene:TNNT2
Gene Synonym:
Exon (9)459..536
Gene:TNNT2
Gene Synonym:
Exon (10)537..647
Gene:TNNT2
Gene Synonym:
Exon (11)648..653
Gene:TNNT2
Gene Synonym:
Exon (12)654..763
Gene:TNNT2
Gene Synonym:
Exon (13)764..854
Gene:TNNT2
Gene Synonym:
Exon (14)855..895
Gene:TNNT2
Gene Synonym:
Exon (15)896..1143
Gene:TNNT2
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, c dbSNP:45590943
11 11 g, t dbSNP:770524611
20 20 c, t dbSNP:746521973
27 27 c, t dbSNP:772939790
45 45 a, g dbSNP:771906661
75 75 a, t dbSNP:747865894
82 82 a, g, t dbSNP:201831895
88 88 a, g dbSNP:730881093
90 90 a, g dbSNP:752977897
92 92 a, c dbSNP:776936911
96 96 c, t dbSNP:765584396
102 102 a, c dbSNP:139705141
113 113 g, t dbSNP:777128825
125 125 c, t dbSNP:148027842
126 126 a, g dbSNP:760247765
131 131 a, g dbSNP:727503516
132 132 a, g dbSNP:772890125
157 157 a, c, g dbSNP:776406819
159 159 a, g dbSNP:770771962
160 160 a, c, t dbSNP:200754249
161 161 a, g dbSNP:200283086
168 168 a, g dbSNP:748078123
178 178 c, t dbSNP:778730615
182 182 a, g dbSNP:756174289
183 183 c, g dbSNP:397516447
191 191 c, t dbSNP:746492909
192 192 a, g dbSNP:757526942
210 210 a, g dbSNP:730881120
213 213 a, g dbSNP:377341875
215 215 c, t dbSNP:771688910
222 222 c, g dbSNP:568628521
224 224 -, aga dbSNP:781616719
227 227 g, t dbSNP:774064875
241 241 a, c dbSNP:768463992
254 254 a, g dbSNP:531954320
255 255 a, c, g dbSNP:141837529
260 260 a, g dbSNP:771980829
269 269 a, g dbSNP:781024179
271 271 a, c dbSNP:371394312
274 274 -, a dbSNP:34008819
275 275 a, c, g dbSNP:727504869
276 276 a, c dbSNP:774702133
277 277 c, t dbSNP:769040140
283 283 c, t dbSNP:761953142
284 284 a, g dbSNP:3729845
292 292 a, c dbSNP:386352374
295 295 a, g dbSNP:397516450
301 301 g, t dbSNP:730881095
302 302 g, t dbSNP:780115529
306 306 a, c dbSNP:730881096
307 307 c, t dbSNP:144900708
311 311 a, g dbSNP:769993507
312 312 a, t dbSNP:746297911
313 313 a, t dbSNP:121964855
315 315 a, c, t dbSNP:397516451
316 316 c, t dbSNP:730881121
317 317 c, g, t dbSNP:140245123
320 320 c, t dbSNP:763782935
321 321 a, g dbSNP:727504255
324 324 a, g dbSNP:727504244
326 326 c, g dbSNP:727503514
328 328 -, g dbSNP:780087395
328 328 c, g dbSNP:397516452
329 329 a, t dbSNP:397516453
334 334 a, c dbSNP:397516455
338 338 g, t dbSNP:730881098
341 341 g, t dbSNP:397516454
344 344 c, t dbSNP:771514445
350 350 c, t dbSNP:267598299
351 351 -, ttc dbSNP:730881117
351 351 c, t dbSNP:397516456
352 352 a, g dbSNP:121964856
354 354 a, c dbSNP:730881122
356 356 a, g dbSNP:778426227
357 357 c, t dbSNP:727503513
358 358 a, g, t dbSNP:397516457
360 360 a, g dbSNP:397516458
361 361 c, t dbSNP:748914885
362 362 -, gga dbSNP:727504277
368 368 g, t dbSNP:397516459
372 372 c, t dbSNP:779857935
376 376 a, g dbSNP:727505027
388 388 c, t dbSNP:727504245
395 395 c, g, t dbSNP:3729547
396 396 a, g, t dbSNP:730881099
402 402 c, t dbSNP:397516460
405 405 a, t dbSNP:121964858
407 407 g, t dbSNP:727504331
422 422 -, a dbSNP:560019679
428 428 a, g dbSNP:201898087
429 429 a, g dbSNP:397516461
434 434 a, g dbSNP:199992160
435 435 c, g dbSNP:767617578
437 437 c, g dbSNP:758543857
438 438 a, g dbSNP:537067344
450 450 a, g dbSNP:765359025
451 451 a, g dbSNP:759758840
453 453 a, t dbSNP:786204405
458 458 c, t dbSNP:727504322
459 459 a, g dbSNP:730881100
463 463 a, g dbSNP:2996496
465 465 c, t dbSNP:397516463
468 468 c, t dbSNP:74315380
469 469 a, c, g dbSNP:397516464
477 477 a, c, g, t dbSNP:45525839
478 478 a, g dbSNP:754037135
479 479 a, g dbSNP:112453991
482 482 c, t dbSNP:375675827
483 483 a, c, g dbSNP:371142225
485 485 g, t dbSNP:141754300
490 490 a, g dbSNP:730880232
492 492 c, t dbSNP:397516465
493 493 a, g dbSNP:397516466
495 495 a, g dbSNP:762340105
497 497 c, t dbSNP:200604266
498 498 a, c, t dbSNP:74315379
498 498 -, c dbSNP:730881115
499 499 a, g dbSNP:730881101
500 500 c, g dbSNP:786204406
502 502 a, g dbSNP:748804579
506 506 a, g dbSNP:775274966
507 507 c, t dbSNP:483352832
508 508 a, g dbSNP:745632066
519 519 c, t dbSNP:730881123
520 520 a, g dbSNP:730881102
521 521 c, g dbSNP:35914325
526 526 a, g dbSNP:770666870
527 527 a, c dbSNP:786205908
528 528 c, t dbSNP:45608937
529 529 a, g dbSNP:201048783
534 534 a, g dbSNP:727505030
544 544 a, g dbSNP:777763436
545 545 a, g dbSNP:750399630
549 549 c, g, t dbSNP:730881103
550 550 a, g dbSNP:397516468
552 552 c, t dbSNP:397516469
553 553 a, g dbSNP:45501500
563 563 gg, tt dbSNP:730881118
564 564 -, gag dbSNP:397516470
565 565 a, g dbSNP:780067626
569 569 a, c, t dbSNP:483352833
573 573 a, g dbSNP:730881104
591 591 g, t dbSNP:730881097
594 594 c, g dbSNP:727503512
595 595 a, g dbSNP:397516471
599 599 a, g dbSNP:566113559
600 600 a, c dbSNP:558549356
605 605 c, g dbSNP:757634539
607 607 a, c dbSNP:397516473
611 611 c, g dbSNP:201270895
612 612 g, t dbSNP:377157235
613 613 c, t dbSNP:727504246
614 614 c, t dbSNP:397516474
617 617 c, t dbSNP:764636823
623 623 a, g dbSNP:763316865
624 624 c, t dbSNP:753292088
630 630 g, t dbSNP:764777623
638 638 c, t dbSNP:759134258
639 639 a, g dbSNP:730881124
646 646 a, c dbSNP:730881105
650 650 c, t dbSNP:767158813
654 654 a, g dbSNP:587782981
657 657 a, g dbSNP:150008205
658 658 -, ag dbSNP:760664767
660 660 c, t dbSNP:730881106
661 661 a, g dbSNP:371047521
672 672 a, g dbSNP:727503511
682 682 c, t dbSNP:397516476
686 686 a, g dbSNP:772300998
687 687 a, c, t dbSNP:45586240
688 688 a, g, t dbSNP:121964860
702 702 -, aag dbSNP:121964859
703 703 -, aga dbSNP:45578238
703 703 a, t dbSNP:190805300
709 709 a, t dbSNP:750800588
714 714 a, g dbSNP:769128006
723 723 a, g dbSNP:397516478
726 726 g, t dbSNP:397516479
736 736 c, t dbSNP:45520032
750 750 a, g dbSNP:730881107
756 756 c, g dbSNP:730881108
767 767 a, g dbSNP:201229194
774 774 a, g dbSNP:746592264
780 780 c, t dbSNP:786204407
790 790 c, g dbSNP:2996495
791 791 c, g dbSNP:3020555
796 796 a, g dbSNP:200500421
806 806 g, t dbSNP:45466197
808 808 c, t dbSNP:369181536
811 811 a, g dbSNP:730881109
816 816 c, g, t dbSNP:730881110
818 818 c, t dbSNP:397516481
819 819 a, g dbSNP:141805127
823 823 a, c, t dbSNP:376037051
825 825 a, c dbSNP:730881111
828 828 a, g dbSNP:727504488
832 832 a, g dbSNP:3730238
847 847 a, t dbSNP:397516482
849 849 c, t dbSNP:730881112
851 851 c, t dbSNP:757379649
856 856 c, t dbSNP:371384395
857 857 c, t dbSNP:761288511
859 859 a, g dbSNP:397516483
860 860 c, t dbSNP:768470426
867 867 a, c dbSNP:748970759
868 868 a, g dbSNP:730881125
869 869 a, c dbSNP:786204408
879 879 a, g, t dbSNP:4523540
881 881 a, c, t dbSNP:376923877
882 882 a, g, t dbSNP:121964861
888 888 -, c dbSNP:730881119
902 902 a, g dbSNP:483352836
905 905 a, c dbSNP:754211195
906 906 c, t dbSNP:121964857
907 907 a, c, g dbSNP:397516484
908 908 c, t dbSNP:45503195
909 909 a, g dbSNP:757664792
910 910 a, g dbSNP:727505233
914 914 a, g dbSNP:752111672
922 922 a, t dbSNP:730880233
926 926 c, t dbSNP:45465693
927 927 a, g dbSNP:147940106
930 930 c, t dbSNP:367785431
931 931 a, g, t dbSNP:141121678
934 934 a, g dbSNP:727504247
935 935 a, g dbSNP:730881116
937 937 a, g dbSNP:770794082
938 938 a, g dbSNP:746858820
944 944 a, c dbSNP:529731863
949 949 c, t dbSNP:768709734
950 950 a, c, t dbSNP:370503996
954 954 g, t dbSNP:756239287
963 963 a, g dbSNP:746131983
967 967 g, t dbSNP:375935487
969 969 c, g, t dbSNP:201271311
971 971 c, t dbSNP:752096322
972 972 -, c dbSNP:776968084
974 974 c, t dbSNP:764761207
975 975 a, g dbSNP:757948388
978 978 c, t dbSNP:149240770
979 979 a, c, g dbSNP:111735792
980 980 c, t dbSNP:553116893
981 981 a, c dbSNP:766030869
990 990 c, t dbSNP:546008385
991 991 a, g dbSNP:773131107
1007 1007 a, g dbSNP:3729998
1012 1012 c, t dbSNP:3730244
1064 1064 c, t dbSNP:185927345
1067 1067 c, g dbSNP:574804643
1091 1091 c, t dbSNP:554553266
1111 1111 c, g dbSNP:534887070

Target ORF information:

RefSeq Version NM_001001432
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001001432

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAGCAGGAG 
GAGGCAGCGG AAGAGGATGC TGAAGCAGAG GCTGAGACCG AGGAGACCAG GGCAGAAGAA
GATGAAGAAG AAGAGGAAGC AAAGGAGGCT GAAGATGGCC CAATGGAGGA GTCCAAACCA
AAGCCCAGGT CGTTCATGCC CAACTTGGTG CCTCCCAAGA TCCCCGATGG AGAGAGAGTG
GACTTTGATG ACATCCACCG GAAGCGCATG GAGAAGGACC TGAATGAGTT GCAGGCGCTG
ATCGAGGCTC ACTTTGAGAA CAGGAAGAAA GAGGAGGAGG AGCTCGTTTC TCTCAAAGAC
AGGATCGAGA GACGTCGGGC AGAGCGGGCC GAGCAGCAGC GCATCCGGAA TGAGCGGGAG
AAGGAGCGGC AGAACCGCCT GGCTGAAGAG AGGGCTCGAC GAGAGGAGGA GGAGAACAGG
AGGAAGGCTG AGGATGAGGC CCGGAAGAAG AAGGCTTTGT CCAACATGAT GCATTTTGGG
GGTTACATCC AGAAGGCCCA GACAGAGCGG AAAAGTGGGA AGAGGCAGAC TGAGCGGGAA
AAGAAGAAGA AGATTCTGGC TGAGAGGAGG AAGGTGCTGG CCATTGACCA CCTGAATGAA
GATCAGCTGA GGGAGAAGGC CAAGGAGCTG TGGCAGAGCA TCTATAACTT GGAGGCAGAG
AAGTTCGACC TGCAGGAGAA GTTCAAGCAG CAGAAATATG AGATCAATGT TCTCCGAAAC
AGGATCAACG ATAACCAGAA AGTCTCCAAG ACCCGCGGGA AGGCTAAAGT CACCGGGCGC
TGGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24695
Accession Version NM_001001430.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 867bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu24695D_COA.pdf (pdf)
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ709280.1, BC002653.2, L40162.1 and AA865519.1. On Feb 5, 2013 this sequence version replaced gi:48255878. Summary: The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 5. It encodes a shorter isoform (2) compared to isoform 5. Variants 2 and 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L40162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG
AGGCAGACTG AGCGGGAAAA GAAGAAGAAG ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC
ATTGACCACC TGAATGAAGA TCAGCTGAGG GAGAAGGCCA AGGAGCTGTG GCAGAGCATC
TATAACTTGG AGGCAGAGAA GTTCGACCTG CAGGAGAAGT TCAAGCAGCA GAAATATGAG
ATCAATGTTC TCCGAAACAG GATCAACGAT AACCAGAAAG TCTCCAAGAC CCGCGGGAAG
GCTAAAGTCA CCGGGCGCTG GAAATAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001001430.1
CDS93..959
Misc Feature(1)75..77(+)
Misc Feature(2)369..761(+)
Misc Feature(3)660..>902(+)
Misc Feature(4)672..674(+)
Misc Feature(5)684..686(+)
Misc Feature(6)699..701(+)
Misc Feature(7)942..944(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..133
Gene:TNNT2
Gene Synonym:
Exon (3)134..144
Gene:TNNT2
Gene Synonym:
Exon (4)145..159
Gene:TNNT2
Gene Synonym:
Exon (5)160..225
Gene:TNNT2
Gene Synonym:
Exon (6)226..261
Gene:TNNT2
Gene Synonym:
Exon (7)262..295
Gene:TNNT2
Gene Synonym:
Exon (8)296..356
Gene:TNNT2
Gene Synonym:
Exon (9)357..473
Gene:TNNT2
Gene Synonym:
Exon (10)474..551
Gene:TNNT2
Gene Synonym:
Exon (11)552..662
Gene:TNNT2
Gene Synonym:
Exon (12)663..671
Gene:TNNT2
Gene Synonym:
Exon (13)672..781
Gene:TNNT2
Gene Synonym:
Exon (14)782..872
Gene:TNNT2
Gene Synonym:
Exon (15)873..913
Gene:TNNT2
Gene Synonym:
Exon (16)914..1161
Gene:TNNT2
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, c dbSNP:45590943
11 11 g, t dbSNP:770524611
20 20 c, t dbSNP:746521973
27 27 c, t dbSNP:772939790
45 45 a, g dbSNP:771906661
75 75 a, t dbSNP:747865894
82 82 a, g, t dbSNP:201831895
88 88 a, g dbSNP:730881093
90 90 a, g dbSNP:752977897
92 92 a, c dbSNP:776936911
96 96 c, t dbSNP:765584396
102 102 a, c dbSNP:139705141
113 113 g, t dbSNP:777128825
125 125 c, t dbSNP:148027842
126 126 a, g dbSNP:760247765
131 131 a, g dbSNP:727503516
132 132 a, g dbSNP:772890125
146 146 a, g dbSNP:754664870
148 148 c, g dbSNP:753645200
151 151 c, t dbSNP:535282031
155 155 g, t dbSNP:397516477
159 159 a, g dbSNP:772966842
172 172 a, c, g dbSNP:776406819
174 174 a, g dbSNP:770771962
175 175 a, c, t dbSNP:200754249
176 176 a, g dbSNP:200283086
183 183 a, g dbSNP:748078123
193 193 c, t dbSNP:778730615
197 197 a, g dbSNP:756174289
198 198 c, g dbSNP:397516447
206 206 c, t dbSNP:746492909
207 207 a, g dbSNP:757526942
225 225 a, g dbSNP:730881120
228 228 a, g dbSNP:377341875
230 230 c, t dbSNP:771688910
237 237 c, g dbSNP:568628521
239 239 -, aga dbSNP:781616719
242 242 g, t dbSNP:774064875
256 256 a, c dbSNP:768463992
269 269 a, g dbSNP:531954320
270 270 a, c, g dbSNP:141837529
275 275 a, g dbSNP:771980829
284 284 a, g dbSNP:781024179
286 286 a, c dbSNP:371394312
289 289 -, a dbSNP:34008819
290 290 a, c, g dbSNP:727504869
291 291 a, c dbSNP:774702133
292 292 c, t dbSNP:769040140
298 298 c, t dbSNP:761953142
299 299 a, g dbSNP:3729845
307 307 a, c dbSNP:386352374
310 310 a, g dbSNP:397516450
316 316 g, t dbSNP:730881095
317 317 g, t dbSNP:780115529
321 321 a, c dbSNP:730881096
322 322 c, t dbSNP:144900708
326 326 a, g dbSNP:769993507
327 327 a, t dbSNP:746297911
328 328 a, t dbSNP:121964855
330 330 a, c, t dbSNP:397516451
331 331 c, t dbSNP:730881121
332 332 c, g, t dbSNP:140245123
335 335 c, t dbSNP:763782935
336 336 a, g dbSNP:727504255
339 339 a, g dbSNP:727504244
341 341 c, g dbSNP:727503514
343 343 -, g dbSNP:780087395
343 343 c, g dbSNP:397516452
344 344 a, t dbSNP:397516453
349 349 a, c dbSNP:397516455
353 353 g, t dbSNP:730881098
356 356 g, t dbSNP:397516454
359 359 c, t dbSNP:771514445
365 365 c, t dbSNP:267598299
366 366 -, ttc dbSNP:730881117
366 366 c, t dbSNP:397516456
367 367 a, g dbSNP:121964856
369 369 a, c dbSNP:730881122
371 371 a, g dbSNP:778426227
372 372 c, t dbSNP:727503513
373 373 a, g, t dbSNP:397516457
375 375 a, g dbSNP:397516458
376 376 c, t dbSNP:748914885
377 377 -, gga dbSNP:727504277
383 383 g, t dbSNP:397516459
387 387 c, t dbSNP:779857935
391 391 a, g dbSNP:727505027
403 403 c, t dbSNP:727504245
410 410 c, g, t dbSNP:3729547
411 411 a, g, t dbSNP:730881099
417 417 c, t dbSNP:397516460
420 420 a, t dbSNP:121964858
422 422 g, t dbSNP:727504331
437 437 -, a dbSNP:560019679
443 443 a, g dbSNP:201898087
444 444 a, g dbSNP:397516461
449 449 a, g dbSNP:199992160
450 450 c, g dbSNP:767617578
452 452 c, g dbSNP:758543857
453 453 a, g dbSNP:537067344
465 465 a, g dbSNP:765359025
466 466 a, g dbSNP:759758840
468 468 a, t dbSNP:786204405
473 473 c, t dbSNP:727504322
474 474 a, g dbSNP:730881100
478 478 a, g dbSNP:2996496
480 480 c, t dbSNP:397516463
483 483 c, t dbSNP:74315380
484 484 a, c, g dbSNP:397516464
492 492 a, c, g, t dbSNP:45525839
493 493 a, g dbSNP:754037135
494 494 a, g dbSNP:112453991
497 497 c, t dbSNP:375675827
498 498 a, c, g dbSNP:371142225
500 500 g, t dbSNP:141754300
505 505 a, g dbSNP:730880232
507 507 c, t dbSNP:397516465
508 508 a, g dbSNP:397516466
510 510 a, g dbSNP:762340105
512 512 c, t dbSNP:200604266
513 513 a, c, t dbSNP:74315379
513 513 -, c dbSNP:730881115
514 514 a, g dbSNP:730881101
515 515 c, g dbSNP:786204406
517 517 a, g dbSNP:748804579
521 521 a, g dbSNP:775274966
522 522 c, t dbSNP:483352832
523 523 a, g dbSNP:745632066
534 534 c, t dbSNP:730881123
535 535 a, g dbSNP:730881102
536 536 c, g dbSNP:35914325
541 541 a, g dbSNP:770666870
542 542 a, c dbSNP:786205908
543 543 c, t dbSNP:45608937
544 544 a, g dbSNP:201048783
549 549 a, g dbSNP:727505030
559 559 a, g dbSNP:777763436
560 560 a, g dbSNP:750399630
564 564 c, g, t dbSNP:730881103
565 565 a, g dbSNP:397516468
567 567 c, t dbSNP:397516469
568 568 a, g dbSNP:45501500
578 578 gg, tt dbSNP:730881118
579 579 -, gag dbSNP:397516470
580 580 a, g dbSNP:780067626
584 584 a, c, t dbSNP:483352833
588 588 a, g dbSNP:730881104
606 606 g, t dbSNP:730881097
609 609 c, g dbSNP:727503512
610 610 a, g dbSNP:397516471
614 614 a, g dbSNP:566113559
615 615 a, c dbSNP:558549356
620 620 c, g dbSNP:757634539
622 622 a, c dbSNP:397516473
626 626 c, g dbSNP:201270895
627 627 g, t dbSNP:377157235
628 628 c, t dbSNP:727504246
629 629 c, t dbSNP:397516474
632 632 c, t dbSNP:764636823
638 638 a, g dbSNP:763316865
639 639 c, t dbSNP:753292088
645 645 g, t dbSNP:764777623
653 653 c, t dbSNP:759134258
654 654 a, g dbSNP:730881124
661 661 a, c dbSNP:730881105
668 668 c, t dbSNP:767158813
672 672 a, g dbSNP:587782981
675 675 a, g dbSNP:150008205
676 676 -, ag dbSNP:760664767
678 678 c, t dbSNP:730881106
679 679 a, g dbSNP:371047521
690 690 a, g dbSNP:727503511
700 700 c, t dbSNP:397516476
704 704 a, g dbSNP:772300998
705 705 a, c, t dbSNP:45586240
706 706 a, g, t dbSNP:121964860
720 720 -, aag dbSNP:121964859
721 721 -, aga dbSNP:45578238
721 721 a, t dbSNP:190805300
727 727 a, t dbSNP:750800588
732 732 a, g dbSNP:769128006
741 741 a, g dbSNP:397516478
744 744 g, t dbSNP:397516479
754 754 c, t dbSNP:45520032
768 768 a, g dbSNP:730881107
774 774 c, g dbSNP:730881108
785 785 a, g dbSNP:201229194
792 792 a, g dbSNP:746592264
798 798 c, t dbSNP:786204407
808 808 c, g dbSNP:2996495
809 809 c, g dbSNP:3020555
814 814 a, g dbSNP:200500421
824 824 g, t dbSNP:45466197
826 826 c, t dbSNP:369181536
829 829 a, g dbSNP:730881109
834 834 c, g, t dbSNP:730881110
836 836 c, t dbSNP:397516481
837 837 a, g dbSNP:141805127
841 841 a, c, t dbSNP:376037051
843 843 a, c dbSNP:730881111
846 846 a, g dbSNP:727504488
850 850 a, g dbSNP:3730238
865 865 a, t dbSNP:397516482
867 867 c, t dbSNP:730881112
869 869 c, t dbSNP:757379649
874 874 c, t dbSNP:371384395
875 875 c, t dbSNP:761288511
877 877 a, g dbSNP:397516483
878 878 c, t dbSNP:768470426
885 885 a, c dbSNP:748970759
886 886 a, g dbSNP:730881125
887 887 a, c dbSNP:786204408
897 897 a, g, t dbSNP:4523540
899 899 a, c, t dbSNP:376923877
900 900 a, g, t dbSNP:121964861
906 906 -, c dbSNP:730881119
920 920 a, g dbSNP:483352836
923 923 a, c dbSNP:754211195
924 924 c, t dbSNP:121964857
925 925 a, c, g dbSNP:397516484
926 926 c, t dbSNP:45503195
927 927 a, g dbSNP:757664792
928 928 a, g dbSNP:727505233
932 932 a, g dbSNP:752111672
940 940 a, t dbSNP:730880233
944 944 c, t dbSNP:45465693
945 945 a, g dbSNP:147940106
948 948 c, t dbSNP:367785431
949 949 a, g, t dbSNP:141121678
952 952 a, g dbSNP:727504247
953 953 a, g dbSNP:730881116
955 955 a, g dbSNP:770794082
956 956 a, g dbSNP:746858820
962 962 a, c dbSNP:529731863
967 967 c, t dbSNP:768709734
968 968 a, c, t dbSNP:370503996
972 972 g, t dbSNP:756239287
981 981 a, g dbSNP:746131983
985 985 g, t dbSNP:375935487
987 987 c, g, t dbSNP:201271311
989 989 c, t dbSNP:752096322
990 990 -, c dbSNP:776968084
992 992 c, t dbSNP:764761207
993 993 a, g dbSNP:757948388
996 996 c, t dbSNP:149240770
997 997 a, c, g dbSNP:111735792
998 998 c, t dbSNP:553116893
999 999 a, c dbSNP:766030869
1008 1008 c, t dbSNP:546008385
1009 1009 a, g dbSNP:773131107
1025 1025 a, g dbSNP:3729998
1030 1030 c, t dbSNP:3730244
1082 1082 c, t dbSNP:185927345
1085 1085 c, g dbSNP:574804643
1109 1109 c, t dbSNP:554553266
1129 1129 c, g dbSNP:534887070

Target ORF information:

RefSeq Version NM_001001430
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001001430

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG
AGGCAGACTG AGCGGGAAAA GAAGAAGAAG ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC
ATTGACCACC TGAATGAAGA TCAGCTGAGG GAGAAGGCCA AGGAGCTGTG GCAGAGCATC
TATAACTTGG AGGCAGAGAA GTTCGACCTG CAGGAGAAGT TCAAGCAGCA GAAATATGAG
ATCAATGTTC TCCGAAACAG GATCAACGAT AACCAGAAAG TCTCCAAGAC CCGCGGGAAG
GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24914
Accession Version NM_001001431.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 858bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu24914D_COA.pdf (pdf)
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ709280.1, BC002653.2 and AA865519.1. On or before Aug 31, 2013 this sequence version replaced gi:530365340, gi:48255880. Summary: The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (3) lacks multiple in-frame exons, compared to variant 5. It encodes a shorter isoform (3) compared to isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002653.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155550, SAMEA2159607 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG ACAGAGCGGA AAAGTGGGAA GAGGCAGACT
GAGCGGGAAA AGAAGAAGAA GATTCTGGCT GAGAGGAGGA AGGTGCTGGC CATTGACCAC
CTGAATGAAG ATCAGCTGAG GGAGAAGGCC AAGGAGCTGT GGCAGAGCAT CTATAACTTG
GAGGCAGAGA AGTTCGACCT GCAGGAGAAG TTCAAGCAGC AGAAATATGA GATCAATGTT
CTCCGAAACA GGATCAACGA TAACCAGAAA GTCTCCAAGA CCCGCGGGAA GGCTAAAGTC
ACCGGGCGCT GGAAATAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001001431.1
CDS93..950
Misc Feature(1)75..77(+)
Misc Feature(2)369..752(+)
Misc Feature(3)663..665(+)
Misc Feature(4)675..677(+)
Misc Feature(5)690..692(+)
Misc Feature(6)933..935(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..133
Gene:TNNT2
Gene Synonym:
Exon (3)134..144
Gene:TNNT2
Gene Synonym:
Exon (4)145..159
Gene:TNNT2
Gene Synonym:
Exon (5)160..225
Gene:TNNT2
Gene Synonym:
Exon (6)226..261
Gene:TNNT2
Gene Synonym:
Exon (7)262..295
Gene:TNNT2
Gene Synonym:
Exon (8)296..356
Gene:TNNT2
Gene Synonym:
Exon (9)357..473
Gene:TNNT2
Gene Synonym:
Exon (10)474..551
Gene:TNNT2
Gene Synonym:
Exon (11)552..662
Gene:TNNT2
Gene Synonym:
Exon (12)663..772
Gene:TNNT2
Gene Synonym:
Exon (13)773..863
Gene:TNNT2
Gene Synonym:
Exon (14)864..904
Gene:TNNT2
Gene Synonym:
Exon (15)905..1152
Gene:TNNT2
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, c dbSNP:45590943
11 11 g, t dbSNP:770524611
20 20 c, t dbSNP:746521973
27 27 c, t dbSNP:772939790
45 45 a, g dbSNP:771906661
75 75 a, t dbSNP:747865894
82 82 a, g, t dbSNP:201831895
88 88 a, g dbSNP:730881093
90 90 a, g dbSNP:752977897
92 92 a, c dbSNP:776936911
96 96 c, t dbSNP:765584396
102 102 a, c dbSNP:139705141
113 113 g, t dbSNP:777128825
125 125 c, t dbSNP:148027842
126 126 a, g dbSNP:760247765
131 131 a, g dbSNP:727503516
132 132 a, g dbSNP:772890125
146 146 a, g dbSNP:754664870
148 148 c, g dbSNP:753645200
151 151 c, t dbSNP:535282031
155 155 g, t dbSNP:397516477
159 159 a, g dbSNP:772966842
172 172 a, c, g dbSNP:776406819
174 174 a, g dbSNP:770771962
175 175 a, c, t dbSNP:200754249
176 176 a, g dbSNP:200283086
183 183 a, g dbSNP:748078123
193 193 c, t dbSNP:778730615
197 197 a, g dbSNP:756174289
198 198 c, g dbSNP:397516447
206 206 c, t dbSNP:746492909
207 207 a, g dbSNP:757526942
225 225 a, g dbSNP:730881120
228 228 a, g dbSNP:377341875
230 230 c, t dbSNP:771688910
237 237 c, g dbSNP:568628521
239 239 -, aga dbSNP:781616719
242 242 g, t dbSNP:774064875
256 256 a, c dbSNP:768463992
269 269 a, g dbSNP:531954320
270 270 a, c, g dbSNP:141837529
275 275 a, g dbSNP:771980829
284 284 a, g dbSNP:781024179
286 286 a, c dbSNP:371394312
289 289 -, a dbSNP:34008819
290 290 a, c, g dbSNP:727504869
291 291 a, c dbSNP:774702133
292 292 c, t dbSNP:769040140
298 298 c, t dbSNP:761953142
299 299 a, g dbSNP:3729845
307 307 a, c dbSNP:386352374
310 310 a, g dbSNP:397516450
316 316 g, t dbSNP:730881095
317 317 g, t dbSNP:780115529
321 321 a, c dbSNP:730881096
322 322 c, t dbSNP:144900708
326 326 a, g dbSNP:769993507
327 327 a, t dbSNP:746297911
328 328 a, t dbSNP:121964855
330 330 a, c, t dbSNP:397516451
331 331 c, t dbSNP:730881121
332 332 c, g, t dbSNP:140245123
335 335 c, t dbSNP:763782935
336 336 a, g dbSNP:727504255
339 339 a, g dbSNP:727504244
341 341 c, g dbSNP:727503514
343 343 -, g dbSNP:780087395
343 343 c, g dbSNP:397516452
344 344 a, t dbSNP:397516453
349 349 a, c dbSNP:397516455
353 353 g, t dbSNP:730881098
356 356 g, t dbSNP:397516454
359 359 c, t dbSNP:771514445
365 365 c, t dbSNP:267598299
366 366 -, ttc dbSNP:730881117
366 366 c, t dbSNP:397516456
367 367 a, g dbSNP:121964856
369 369 a, c dbSNP:730881122
371 371 a, g dbSNP:778426227
372 372 c, t dbSNP:727503513
373 373 a, g, t dbSNP:397516457
375 375 a, g dbSNP:397516458
376 376 c, t dbSNP:748914885
377 377 -, gga dbSNP:727504277
383 383 g, t dbSNP:397516459
387 387 c, t dbSNP:779857935
391 391 a, g dbSNP:727505027
403 403 c, t dbSNP:727504245
410 410 c, g, t dbSNP:3729547
411 411 a, g, t dbSNP:730881099
417 417 c, t dbSNP:397516460
420 420 a, t dbSNP:121964858
422 422 g, t dbSNP:727504331
437 437 -, a dbSNP:560019679
443 443 a, g dbSNP:201898087
444 444 a, g dbSNP:397516461
449 449 a, g dbSNP:199992160
450 450 c, g dbSNP:767617578
452 452 c, g dbSNP:758543857
453 453 a, g dbSNP:537067344
465 465 a, g dbSNP:765359025
466 466 a, g dbSNP:759758840
468 468 a, t dbSNP:786204405
473 473 c, t dbSNP:727504322
474 474 a, g dbSNP:730881100
478 478 a, g dbSNP:2996496
480 480 c, t dbSNP:397516463
483 483 c, t dbSNP:74315380
484 484 a, c, g dbSNP:397516464
492 492 a, c, g, t dbSNP:45525839
493 493 a, g dbSNP:754037135
494 494 a, g dbSNP:112453991
497 497 c, t dbSNP:375675827
498 498 a, c, g dbSNP:371142225
500 500 g, t dbSNP:141754300
505 505 a, g dbSNP:730880232
507 507 c, t dbSNP:397516465
508 508 a, g dbSNP:397516466
510 510 a, g dbSNP:762340105
512 512 c, t dbSNP:200604266
513 513 a, c, t dbSNP:74315379
513 513 -, c dbSNP:730881115
514 514 a, g dbSNP:730881101
515 515 c, g dbSNP:786204406
517 517 a, g dbSNP:748804579
521 521 a, g dbSNP:775274966
522 522 c, t dbSNP:483352832
523 523 a, g dbSNP:745632066
534 534 c, t dbSNP:730881123
535 535 a, g dbSNP:730881102
536 536 c, g dbSNP:35914325
541 541 a, g dbSNP:770666870
542 542 a, c dbSNP:786205908
543 543 c, t dbSNP:45608937
544 544 a, g dbSNP:201048783
549 549 a, g dbSNP:727505030
559 559 a, g dbSNP:777763436
560 560 a, g dbSNP:750399630
564 564 c, g, t dbSNP:730881103
565 565 a, g dbSNP:397516468
567 567 c, t dbSNP:397516469
568 568 a, g dbSNP:45501500
578 578 gg, tt dbSNP:730881118
579 579 -, gag dbSNP:397516470
580 580 a, g dbSNP:780067626
584 584 a, c, t dbSNP:483352833
588 588 a, g dbSNP:730881104
606 606 g, t dbSNP:730881097
609 609 c, g dbSNP:727503512
610 610 a, g dbSNP:397516471
614 614 a, g dbSNP:566113559
615 615 a, c dbSNP:558549356
620 620 c, g dbSNP:757634539
622 622 a, c dbSNP:397516473
626 626 c, g dbSNP:201270895
627 627 g, t dbSNP:377157235
628 628 c, t dbSNP:727504246
629 629 c, t dbSNP:397516474
632 632 c, t dbSNP:764636823
638 638 a, g dbSNP:763316865
639 639 c, t dbSNP:753292088
645 645 g, t dbSNP:764777623
653 653 c, t dbSNP:759134258
654 654 a, g dbSNP:730881124
661 661 a, c dbSNP:730881105
663 663 a, g dbSNP:587782981
666 666 a, g dbSNP:150008205
667 667 -, ag dbSNP:760664767
669 669 c, t dbSNP:730881106
670 670 a, g dbSNP:371047521
681 681 a, g dbSNP:727503511
691 691 c, t dbSNP:397516476
695 695 a, g dbSNP:772300998
696 696 a, c, t dbSNP:45586240
697 697 a, g, t dbSNP:121964860
711 711 -, aag dbSNP:121964859
712 712 -, aga dbSNP:45578238
712 712 a, t dbSNP:190805300
718 718 a, t dbSNP:750800588
723 723 a, g dbSNP:769128006
732 732 a, g dbSNP:397516478
735 735 g, t dbSNP:397516479
745 745 c, t dbSNP:45520032
759 759 a, g dbSNP:730881107
765 765 c, g dbSNP:730881108
776 776 a, g dbSNP:201229194
783 783 a, g dbSNP:746592264
789 789 c, t dbSNP:786204407
799 799 c, g dbSNP:2996495
800 800 c, g dbSNP:3020555
805 805 a, g dbSNP:200500421
815 815 g, t dbSNP:45466197
817 817 c, t dbSNP:369181536
820 820 a, g dbSNP:730881109
825 825 c, g, t dbSNP:730881110
827 827 c, t dbSNP:397516481
828 828 a, g dbSNP:141805127
832 832 a, c, t dbSNP:376037051
834 834 a, c dbSNP:730881111
837 837 a, g dbSNP:727504488
841 841 a, g dbSNP:3730238
856 856 a, t dbSNP:397516482
858 858 c, t dbSNP:730881112
860 860 c, t dbSNP:757379649
865 865 c, t dbSNP:371384395
866 866 c, t dbSNP:761288511
868 868 a, g dbSNP:397516483
869 869 c, t dbSNP:768470426
876 876 a, c dbSNP:748970759
877 877 a, g dbSNP:730881125
878 878 a, c dbSNP:786204408
888 888 a, g, t dbSNP:4523540
890 890 a, c, t dbSNP:376923877
891 891 a, g, t dbSNP:121964861
897 897 -, c dbSNP:730881119
911 911 a, g dbSNP:483352836
914 914 a, c dbSNP:754211195
915 915 c, t dbSNP:121964857
916 916 a, c, g dbSNP:397516484
917 917 c, t dbSNP:45503195
918 918 a, g dbSNP:757664792
919 919 a, g dbSNP:727505233
923 923 a, g dbSNP:752111672
931 931 a, t dbSNP:730880233
935 935 c, t dbSNP:45465693
936 936 a, g dbSNP:147940106
939 939 c, t dbSNP:367785431
940 940 a, g, t dbSNP:141121678
943 943 a, g dbSNP:727504247
944 944 a, g dbSNP:730881116
946 946 a, g dbSNP:770794082
947 947 a, g dbSNP:746858820
953 953 a, c dbSNP:529731863
958 958 c, t dbSNP:768709734
959 959 a, c, t dbSNP:370503996
963 963 g, t dbSNP:756239287
972 972 a, g dbSNP:746131983
976 976 g, t dbSNP:375935487
978 978 c, g, t dbSNP:201271311
980 980 c, t dbSNP:752096322
981 981 -, c dbSNP:776968084
983 983 c, t dbSNP:764761207
984 984 a, g dbSNP:757948388
987 987 c, t dbSNP:149240770
988 988 a, c, g dbSNP:111735792
989 989 c, t dbSNP:553116893
990 990 a, c dbSNP:766030869
999 999 c, t dbSNP:546008385
1000 1000 a, g dbSNP:773131107
1016 1016 a, g dbSNP:3729998
1021 1021 c, t dbSNP:3730244
1073 1073 c, t dbSNP:185927345
1076 1076 c, g dbSNP:574804643
1100 1100 c, t dbSNP:554553266
1120 1120 c, g dbSNP:534887070

Target ORF information:

RefSeq Version NM_001001431
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001001431

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG ACAGAGCGGA AAAGTGGGAA GAGGCAGACT
GAGCGGGAAA AGAAGAAGAA GATTCTGGCT GAGAGGAGGA AGGTGCTGGC CATTGACCAC
CTGAATGAAG ATCAGCTGAG GGAGAAGGCC AAGGAGCTGT GGCAGAGCAT CTATAACTTG
GAGGCAGAGA AGTTCGACCT GCAGGAGAAG TTCAAGCAGC AGAAATATGA GATCAATGTT
CTCCGAAACA GGATCAACGA TAACCAGAAA GTCTCCAAGA CCCGCGGGAA GGCTAAAGTC
ACCGGGCGCT GGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25054
Accession Version XM_011509938.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 897bp)
Protein sequence