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TP53 cDNA ORF clone, Homo sapiens (human)

Gene Symbol TP53
Entrez Gene ID 7157
Full Name tumor protein p53
Synonyms BCC7, LFS1, P53, TRP53
General protein information
Preferred Names
cellular tumor antigen p53
Names
cellular tumor antigen p53
antigen NY-CO-13
phosphoprotein p53
p53 tumor suppressor
transformation-related protein 53
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17p13.1

Summary This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]. lac of sum
Disorder MIM:

191170

Disorder Html: Colorectal cancer, 114500 (3); Li-Fraumeni syndrome, 151623 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_001276697 NP_001263626 cellular tumor antigen p53 isoform j
NM_001276698 NP_001263627 cellular tumor antigen p53 isoform k
NM_001276699 NP_001263628 cellular tumor antigen p53 isoform l
NM_001126115 NP_001119587 cellular tumor antigen p53 isoform d
NM_001126116 NP_001119588 cellular tumor antigen p53 isoform e
NM_001126117 NP_001119589 cellular tumor antigen p53 isoform f
NM_000546 NP_000537 cellular tumor antigen p53 isoform a
NM_001126112 NP_001119584 cellular tumor antigen p53 isoform a
NM_001126113 NP_001119585 cellular tumor antigen p53 isoform c
NM_001126114 NP_001119586 cellular tumor antigen p53 isoform b
NM_001126118 NP_001119590 cellular tumor antigen p53 isoform g
NM_001276695 NP_001263624 cellular tumor antigen p53 isoform h
NM_001276696 NP_001263625 cellular tumor antigen p53 isoform i
NM_001276760 NP_001263689 cellular tumor antigen p53 isoform g
NM_001276761 NP_001263690 cellular tumor antigen p53 isoform g



Homo sapiens (human) TP53 NP_000537.3
Pan troglodytes (chimpanzee) TP53 XP_001172077.2
Macaca mulatta (Rhesus monkey) TP53 NP_001040616.1
Canis lupus familiaris (dog) TP53 NP_001003210.1
Bos taurus (cattle) TP53 NP_776626.1
Mus musculus (house mouse) Trp53 NP_035770.2
Rattus norvegicus (Norway rat) Tp53 NP_112251.2
Danio rerio (zebrafish) tp53 NP_001258749.1
Xenopus (Silurana) tropicalis (western clawed frog) tp53 NP_001001903.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TP53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TP53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20389
NM_001276697 Homo sapiens tumor protein p53 (TP53), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu20873
NM_001276698 Homo sapiens tumor protein p53 (TP53), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu21229
NM_001276699 Homo sapiens tumor protein p53 (TP53), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu19337
NM_001126115 Homo sapiens tumor protein p53 (TP53), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu18420
NM_001126116 Homo sapiens tumor protein p53 (TP53), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu18623
NM_001126117 Homo sapiens tumor protein p53 (TP53), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu20059
NM_000546 Homo sapiens tumor protein p53 (TP53), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu20059
NM_001126112 Homo sapiens tumor protein p53 (TP53), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu20186
NM_001126113 Homo sapiens tumor protein p53 (TP53), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$144.50-$202.30
$289.00
OHu20211
NM_001126114 Homo sapiens tumor protein p53 (TP53), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu16777
NM_001126118 Homo sapiens tumor protein p53 (TP53), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu16674
NM_001276695 Homo sapiens tumor protein p53 (TP53), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu16815
NM_001276696 Homo sapiens tumor protein p53 (TP53), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu16777
NM_001276760 Homo sapiens tumor protein p53 (TP53), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu16777
NM_001276761 Homo sapiens tumor protein p53 (TP53), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20389
Clone ID Related Accession (Same CDS sequence) NM_001276697
Accession Version NM_001276697.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 705bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform j
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087388.9, DQ186650.1 and AK223026.1. Transcript Variant: This variant (5) uses an alternate promoter and lacks multiple 5' exons, compared to variant 1. This variant can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (j, also known as delta160p53alpha) results from translation initiation at the downstream start codon. It has a shorter N-terminus, compared to isoform a. This variant is supported by data in PMID:16131611. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:20937277) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: DQ186650.1, BM467806.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT 
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGATCC GTGGGCGTGA GCGCTTCGAG
ATGTTCCGAG AGCTGAATGA GGCCTTGGAA CTCAAGGATG CCCAGGCTGG GAAGGAGCCA
GGGGGGAGCA GGGCTCACTC CAGCCACCTG AAGTCCAAAA AGGGTCAGTC TACCTCCCGC
CATAAAAAAC TCATGTTCAA GACAGAAGGG CCTGACTCAG ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001263626.1
CDS360..1064
Misc Feature(1)135..137(+)
Misc Feature(2)279..281(+)
Misc Feature(3)360..746(+)
Misc Feature(4)408..608(+)
Misc Feature(5)411..425(+)
Misc Feature(6)597..722(+)
Misc Feature(7)852..959(+)
Exon (1)1..441
Gene:TP53
Gene Synonym:
Exon (2)442..554
Gene:TP53
Gene Synonym:
Exon (3)555..664
Gene:TP53
Gene Synonym:
Exon (4)665..801
Gene:TP53
Gene Synonym:
Exon (5)802..875
Gene:TP53
Gene Synonym:
Exon (6)876..982
Gene:TP53
Gene Synonym:
Exon (7)983..2271
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001276697
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276697

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT 
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGATCC GTGGGCGTGA GCGCTTCGAG
ATGTTCCGAG AGCTGAATGA GGCCTTGGAA CTCAAGGATG CCCAGGCTGG GAAGGAGCCA
GGGGGGAGCA GGGCTCACTC CAGCCACCTG AAGTCCAAAA AGGGTCAGTC TACCTCCCGC
CATAAAAAAC TCATGTTCAA GACAGAAGGG CCTGACTCAG ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20873
Clone ID Related Accession (Same CDS sequence) NM_001276698
Accession Version NM_001276698.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 549bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform k
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087388.9, DQ186651.1 and AK223026.1. Transcript Variant: This variant (6) uses an alternate promoter and lacks multiple 5' exons, compared to variant 1. This variant can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (k, also known as delta160p53beta) results from translation initiation at the downstream start codon. It has a shorter N-terminus and a distinct C-terminus, compared to isoform a. This variant is supported by data in PMID:16131611. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:20937277) NMD candidate :: translation inferred ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: DQ186651.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT 
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGGACC AGACCAGCTT TCAAAAAGAA
AATTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001263627.1
CDS360..908
Misc Feature(1)135..137(+)
Misc Feature(2)279..281(+)
Misc Feature(3)360..746(+)
Misc Feature(4)408..608(+)
Misc Feature(5)411..425(+)
Misc Feature(6)597..722(+)
Exon (1)1..441
Gene:TP53
Gene Synonym:
Exon (2)442..554
Gene:TP53
Gene Synonym:
Exon (3)555..664
Gene:TP53
Gene Synonym:
Exon (4)665..801
Gene:TP53
Gene Synonym:
Exon (5)802..875
Gene:TP53
Gene Synonym:
Exon (6)876..1008
Gene:TP53
Gene Synonym:
Exon (7)1009..1115
Gene:TP53
Gene Synonym:
Exon (8)1116..2404
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001276698
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276698

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT 
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGGACC AGACCAGCTT TCAAAAAGAA
AATTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21229
Clone ID Related Accession (Same CDS sequence) NM_001276699
Accession Version NM_001276699.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 564bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform l
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087388.9, DQ186652.1 and AK223026.1. Transcript Variant: This variant (7) uses an alternate promoter and lacks multiple 5' exons, compared to variant 1. This variant can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (l, also known as delta160p53gamma) results from translation initiation at the downstream start codon. It has a shorter N-terminus and a distinct C-terminus, compared to isoform a. This variant is supported by data in PMID:16131611. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:20937277) NMD candidate :: translation inferred ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: DQ186652.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2155371 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT 
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGATGC TACTTGACTT ACGATGGTGT
TACTTCCTGA TAAACTCGTC GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001263628.1
CDS360..923
Misc Feature(1)135..137(+)
Misc Feature(2)279..281(+)
Misc Feature(3)360..746(+)
Misc Feature(4)408..608(+)
Misc Feature(5)411..425(+)
Misc Feature(6)597..722(+)
Exon (1)1..441
Gene:TP53
Gene Synonym:
Exon (2)442..554
Gene:TP53
Gene Synonym:
Exon (3)555..664
Gene:TP53
Gene Synonym:
Exon (4)665..801
Gene:TP53
Gene Synonym:
Exon (5)802..875
Gene:TP53
Gene Synonym:
Exon (6)876..935
Gene:TP53
Gene Synonym:
Exon (7)936..1042
Gene:TP53
Gene Synonym:
Exon (8)1043..2331
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001276699
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276699

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT 
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGATGC TACTTGACTT ACGATGGTGT
TACTTCCTGA TAAACTCGTC GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19337
Clone ID Related Accession (Same CDS sequence) NM_001126115
Accession Version NM_001126115.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform d
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087388.9, DQ186650.1 and AK223026.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (5) uses an alternate promoter and lacks multiple 5' exons, compared to variant 1. This variant can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (d, also known as delta133p53alpha) results from translation initiation at the upstream start codon. It has a shorter N-terminus, compared to isoform a. This variant is supported by data in PMID:16131611. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ186650.1, BM467806.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTTTGCC AACTGGCCAA GACCTGCCCT GTGCAGCTGT GGGTTGATTC CACACCCCCG 
CCCGGCACCC GCGTCCGCGC CATGGCCATC TACAAGCAGT CACAGCACAT GACGGAGGTT
GTGAGGCGCT GCCCCCACCA TGAGCGCTGC TCAGATAGCG ATGGTCTGGC CCCTCCTCAG
CATCTTATCC GAGTGGAAGG AAATTTGCGT GTGGAGTATT TGGATGACAG AAACACTTTT
CGACATAGTG TGGTGGTGCC CTATGAGCCG CCTGAGGTTG GCTCTGACTG TACCACCATC
CACTACAACT ACATGTGTAA CAGTTCCTGC ATGGGCGGCA TGAACCGGAG GCCCATCCTC
ACCATCATCA CACTGGAAGA CTCCAGTGGT AATCTACTGG GACGGAACAG CTTTGAGGTG
CGTGTTTGTG CCTGTCCTGG GAGAGACCGG CGCACAGAGG AAGAGAATCT CCGCAAGAAA
GGGGAGCCTC ACCACGAGCT GCCCCCAGGG AGCACTAAGC GAGCACTGCC CAACAACACC
AGCTCCTCTC CCCAGCCAAA GAAGAAACCA CTGGATGGAG AATATTTCAC CCTTCAGATC
CGTGGGCGTG AGCGCTTCGA GATGTTCCGA GAGCTGAATG AGGCCTTGGA ACTCAAGGAT
GCCCAGGCTG GGAAGGAGCC AGGGGGGAGC AGGGCTCACT CCAGCCACCT GAAGTCCAAA
AAGGGTCAGT CTACCTCCCG CCATAAAAAA CTCATGTTCA AGACAGAAGG GCCTGACTCA
GACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001119587.1
CDS279..1064
Misc Feature(1)135..137(+)
Misc Feature(2)279..746(+)
Misc Feature(3)360..362(+)
Misc Feature(4)408..608(+)
Misc Feature(5)411..425(+)
Misc Feature(6)597..722(+)
Misc Feature(7)852..959(+)
Exon (1)1..441
Gene:TP53
Gene Synonym:
Exon (2)442..554
Gene:TP53
Gene Synonym:
Exon (3)555..664
Gene:TP53
Gene Synonym:
Exon (4)665..801
Gene:TP53
Gene Synonym:
Exon (5)802..875
Gene:TP53
Gene Synonym:
Exon (6)876..982
Gene:TP53
Gene Synonym:
Exon (7)983..2271
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001126115
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001126115

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTTTGCC AACTGGCCAA GACCTGCCCT GTGCAGCTGT GGGTTGATTC CACACCCCCG 
CCCGGCACCC GCGTCCGCGC CATGGCCATC TACAAGCAGT CACAGCACAT GACGGAGGTT
GTGAGGCGCT GCCCCCACCA TGAGCGCTGC TCAGATAGCG ATGGTCTGGC CCCTCCTCAG
CATCTTATCC GAGTGGAAGG AAATTTGCGT GTGGAGTATT TGGATGACAG AAACACTTTT
CGACATAGTG TGGTGGTGCC CTATGAGCCG CCTGAGGTTG GCTCTGACTG TACCACCATC
CACTACAACT ACATGTGTAA CAGTTCCTGC ATGGGCGGCA TGAACCGGAG GCCCATCCTC
ACCATCATCA CACTGGAAGA CTCCAGTGGT AATCTACTGG GACGGAACAG CTTTGAGGTG
CGTGTTTGTG CCTGTCCTGG GAGAGACCGG CGCACAGAGG AAGAGAATCT CCGCAAGAAA
GGGGAGCCTC ACCACGAGCT GCCCCCAGGG AGCACTAAGC GAGCACTGCC CAACAACACC
AGCTCCTCTC CCCAGCCAAA GAAGAAACCA CTGGATGGAG AATATTTCAC CCTTCAGATC
CGTGGGCGTG AGCGCTTCGA GATGTTCCGA GAGCTGAATG AGGCCTTGGA ACTCAAGGAT
GCCCAGGCTG GGAAGGAGCC AGGGGGGAGC AGGGCTCACT CCAGCCACCT GAAGTCCAAA
AAGGGTCAGT CTACCTCCCG CCATAAAAAA CTCATGTTCA AGACAGAAGG GCCTGACTCA
GACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18420
Clone ID Related Accession (Same CDS sequence) NM_001126116
Accession Version NM_001126116.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform e
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087388.9, DQ186651.1 and AK223026.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (6) uses an alternate promoter and lacks multiple 5' exons, compared to variant 1. This variant can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (e, also known as delta133p53beta) results from translation initiation at the upstream start codon. It has a shorter N-terminus and a distinct C-terminus, compared to isoform a. This variant is supported by data in PMID:16131611. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## NMD candidate :: PMID: 16131611, 16601753, 18289041 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: DQ186651.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGTTTTGCC AACTGGCCAA GACCTGCCCT GTGCAGCTGT GGGTTGATTC CACACCCCCG 
CCCGGCACCC GCGTCCGCGC CATGGCCATC TACAAGCAGT CACAGCACAT GACGGAGGTT
GTGAGGCGCT GCCCCCACCA TGAGCGCTGC TCAGATAGCG ATGGTCTGGC CCCTCCTCAG
CATCTTATCC GAGTGGAAGG AAATTTGCGT GTGGAGTATT TGGATGACAG AAACACTTTT
CGACATAGTG TGGTGGTGCC CTATGAGCCG CCTGAGGTTG GCTCTGACTG TACCACCATC
CACTACAACT ACATGTGTAA CAGTTCCTGC ATGGGCGGCA TGAACCGGAG GCCCATCCTC
ACCATCATCA CACTGGAAGA CTCCAGTGGT AATCTACTGG GACGGAACAG CTTTGAGGTG
CGTGTTTGTG CCTGTCCTGG GAGAGACCGG CGCACAGAGG AAGAGAATCT CCGCAAGAAA
GGGGAGCCTC ACCACGAGCT GCCCCCAGGG AGCACTAAGC GAGCACTGCC CAACAACACC
AGCTCCTCTC CCCAGCCAAA GAAGAAACCA CTGGATGGAG AATATTTCAC CCTTCAGGAC
CAGACCAGCT TTCAAAAAGA AAATTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001119588.1
CDS279..908
Misc Feature(1)135..137(+)
Misc Feature(2)279..746(+)
Misc Feature(3)360..362(+)
Misc Feature(4)408..608(+)
Misc Feature(5)411..425(+)
Misc Feature(6)597..722(+)
Exon (1)1..441
Gene:TP53
Gene Synonym:
Exon (2)442..554
Gene:TP53
Gene Synonym:
Exon (3)555..664
Gene:TP53
Gene Synonym:
Exon (4)665..801
Gene:TP53
Gene Synonym:
Exon (5)802..875
Gene:TP53
Gene Synonym:
Exon (6)876..1008
Gene:TP53
Gene Synonym:
Exon (7)1009..1115
Gene:TP53
Gene Synonym:
Exon (8)1116..2404
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001126116
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001126116

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGTTTTGCC AACTGGCCAA GACCTGCCCT GTGCAGCTGT GGGTTGATTC CACACCCCCG 
CCCGGCACCC GCGTCCGCGC CATGGCCATC TACAAGCAGT CACAGCACAT GACGGAGGTT
GTGAGGCGCT GCCCCCACCA TGAGCGCTGC TCAGATAGCG ATGGTCTGGC CCCTCCTCAG
CATCTTATCC GAGTGGAAGG AAATTTGCGT GTGGAGTATT TGGATGACAG AAACACTTTT
CGACATAGTG TGGTGGTGCC CTATGAGCCG CCTGAGGTTG GCTCTGACTG TACCACCATC
CACTACAACT ACATGTGTAA CAGTTCCTGC ATGGGCGGCA TGAACCGGAG GCCCATCCTC
ACCATCATCA CACTGGAAGA CTCCAGTGGT AATCTACTGG GACGGAACAG CTTTGAGGTG
CGTGTTTGTG CCTGTCCTGG GAGAGACCGG CGCACAGAGG AAGAGAATCT CCGCAAGAAA
GGGGAGCCTC ACCACGAGCT GCCCCCAGGG AGCACTAAGC GAGCACTGCC CAACAACACC
AGCTCCTCTC CCCAGCCAAA GAAGAAACCA CTGGATGGAG AATATTTCAC CCTTCAGGAC
CAGACCAGCT TTCAAAAAGA AAATTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18623
Clone ID Related Accession (Same CDS sequence) NM_001126117
Accession Version NM_001126117.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 645bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform f
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087388.9, DQ186652.1 and AK223026.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (7) uses an alternate promoter and lacks multiple 5' exons, compared to variant 1. This variant can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (f, also known as delta133p53gamma) results from translation initiation at the upstream start codon. It has a shorter N-terminus and a distinct C-terminus, compared to isoform a. This variant is supported by data in PMID:16131611. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## NMD candidate :: translation inferred ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: DQ186652.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2155371 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGTTTTGCC AACTGGCCAA GACCTGCCCT GTGCAGCTGT GGGTTGATTC CACACCCCCG 
CCCGGCACCC GCGTCCGCGC CATGGCCATC TACAAGCAGT CACAGCACAT GACGGAGGTT
GTGAGGCGCT GCCCCCACCA TGAGCGCTGC TCAGATAGCG ATGGTCTGGC CCCTCCTCAG
CATCTTATCC GAGTGGAAGG AAATTTGCGT GTGGAGTATT TGGATGACAG AAACACTTTT
CGACATAGTG TGGTGGTGCC CTATGAGCCG CCTGAGGTTG GCTCTGACTG TACCACCATC
CACTACAACT ACATGTGTAA CAGTTCCTGC ATGGGCGGCA TGAACCGGAG GCCCATCCTC
ACCATCATCA CACTGGAAGA CTCCAGTGGT AATCTACTGG GACGGAACAG CTTTGAGGTG
CGTGTTTGTG CCTGTCCTGG GAGAGACCGG CGCACAGAGG AAGAGAATCT CCGCAAGAAA
GGGGAGCCTC ACCACGAGCT GCCCCCAGGG AGCACTAAGC GAGCACTGCC CAACAACACC
AGCTCCTCTC CCCAGCCAAA GAAGAAACCA CTGGATGGAG AATATTTCAC CCTTCAGATG
CTACTTGACT TACGATGGTG TTACTTCCTG ATAAACTCGT CGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001119589.1
CDS279..923
Misc Feature(1)135..137(+)
Misc Feature(2)279..746(+)
Misc Feature(3)360..362(+)
Misc Feature(4)408..608(+)
Misc Feature(5)411..425(+)
Misc Feature(6)597..722(+)
Exon (1)1..441
Gene:TP53
Gene Synonym:
Exon (2)442..554
Gene:TP53
Gene Synonym:
Exon (3)555..664
Gene:TP53
Gene Synonym:
Exon (4)665..801
Gene:TP53
Gene Synonym:
Exon (5)802..875
Gene:TP53
Gene Synonym:
Exon (6)876..935
Gene:TP53
Gene Synonym:
Exon (7)936..1042
Gene:TP53
Gene Synonym:
Exon (8)1043..2331
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001126117
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001126117

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGTTTTGCC AACTGGCCAA GACCTGCCCT GTGCAGCTGT GGGTTGATTC CACACCCCCG 
CCCGGCACCC GCGTCCGCGC CATGGCCATC TACAAGCAGT CACAGCACAT GACGGAGGTT
GTGAGGCGCT GCCCCCACCA TGAGCGCTGC TCAGATAGCG ATGGTCTGGC CCCTCCTCAG
CATCTTATCC GAGTGGAAGG AAATTTGCGT GTGGAGTATT TGGATGACAG AAACACTTTT
CGACATAGTG TGGTGGTGCC CTATGAGCCG CCTGAGGTTG GCTCTGACTG TACCACCATC
CACTACAACT ACATGTGTAA CAGTTCCTGC ATGGGCGGCA TGAACCGGAG GCCCATCCTC
ACCATCATCA CACTGGAAGA CTCCAGTGGT AATCTACTGG GACGGAACAG CTTTGAGGTG
CGTGTTTGTG CCTGTCCTGG GAGAGACCGG CGCACAGAGG AAGAGAATCT CCGCAAGAAA
GGGGAGCCTC ACCACGAGCT GCCCCCAGGG AGCACTAAGC GAGCACTGCC CAACAACACC
AGCTCCTCTC CCCAGCCAAA GAAGAAACCA CTGGATGGAG AATATTTCAC CCTTCAGATG
CTACTTGACT TACGATGGTG TTACTTCCTG ATAAACTCGT CGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20059
Clone ID Related Accession (Same CDS sequence) NM_000546 , NM_001126112
Accession Version NM_000546.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA453049.1, X02469.1, AK223026.1 and DQ186650.1. This sequence is a reference standard in the RefSeqGene project. On Jan 7, 2012 this sequence version replaced gi:187830767. Transcript Variant: This variant (1) can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (a, also known as p53alpha) results from translation initiation at the upstream start codon. Both variants 1 and 2 encode isoform a, which is the longest isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ191317.1, DQ286964.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAGGAGC CGCAGTCAGA TCCTAGCGTC GAGCCCCCTC TGAGTCAGGA AACATTTTCA 
GACCTATGGA AACTACTTCC TGAAAACAAC GTTCTGTCCC CCTTGCCGTC CCAAGCAATG
GATGATTTGA TGCTGTCCCC GGACGATATT GAACAATGGT TCACTGAAGA CCCAGGTCCA
GATGAAGCTC CCAGAATGCC AGAGGCTGCT CCCCCCGTGG CCCCTGCACC AGCAGCTCCT
ACACCGGCGG CCCCTGCACC AGCCCCCTCC TGGCCCCTGT CATCTTCTGT CCCTTCCCAG
AAAACCTACC AGGGCAGCTA CGGTTTCCGT CTGGGCTTCT TGCATTCTGG GACAGCCAAG
TCTGTGACTT GCACGTACTC CCCTGCCCTC AACAAGATGT TTTGCCAACT GGCCAAGACC
TGCCCTGTGC AGCTGTGGGT TGATTCCACA CCCCCGCCCG GCACCCGCGT CCGCGCCATG
GCCATCTACA AGCAGTCACA GCACATGACG GAGGTTGTGA GGCGCTGCCC CCACCATGAG
CGCTGCTCAG ATAGCGATGG TCTGGCCCCT CCTCAGCATC TTATCCGAGT GGAAGGAAAT
TTGCGTGTGG AGTATTTGGA TGACAGAAAC ACTTTTCGAC ATAGTGTGGT GGTGCCCTAT
GAGCCGCCTG AGGTTGGCTC TGACTGTACC ACCATCCACT ACAACTACAT GTGTAACAGT
TCCTGCATGG GCGGCATGAA CCGGAGGCCC ATCCTCACCA TCATCACACT GGAAGACTCC
AGTGGTAATC TACTGGGACG GAACAGCTTT GAGGTGCGTG TTTGTGCCTG TCCTGGGAGA
GACCGGCGCA CAGAGGAAGA GAATCTCCGC AAGAAAGGGG AGCCTCACCA CGAGCTGCCC
CCAGGGAGCA CTAAGCGAGC ACTGCCCAAC AACACCAGCT CCTCTCCCCA GCCAAAGAAG
AAACCACTGG ATGGAGAATA TTTCACCCTT CAGATCCGTG GGCGTGAGCG CTTCGAGATG
TTCCGAGAGC TGAATGAGGC CTTGGAACTC AAGGATGCCC AGGCTGGGAA GGAGCCAGGG
GGGAGCAGGG CTCACTCCAG CCACCTGAAG TCCAAAAAGG GTCAGTCTAC CTCCCGCCAT
AAAAAACTCA TGTTCAAGAC AGAAGGGCCT GACTCAGACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000537.3
CDS203..1384
Misc Feature(1)95..97(+)
Misc Feature(2)203..451(+)
Misc Feature(3)203..334(+)
Misc Feature(4)215..289(+)
Misc Feature(5)218..220(+)
Misc Feature(6)227..229(+)
Misc Feature(7)227..229(+)
Misc Feature(8)227..229(+)
Misc Feature(9)245..247(+)
Misc Feature(10)245..247(+)
Misc Feature(11)245..247(+)
Misc Feature(12)245..247(+)
Misc Feature(13)245..247(+)
Misc Feature(14)245..247(+)
Misc Feature(15)245..247(+)
Misc Feature(16)245..247(+)
Misc Feature(17)251..277(+)
Misc Feature(18)254..256(+)
Misc Feature(19)254..256(+)
Misc Feature(20)254..256(+)
Misc Feature(21)254..256(+)
Misc Feature(22)254..256(+)
Misc Feature(23)254..256(+)
Misc Feature(24)254..256(+)
Misc Feature(25)254..256(+)
Misc Feature(26)260..262(+)
Misc Feature(27)260..262(+)
Misc Feature(28)260..262(+)
Misc Feature(29)260..262(+)
Misc Feature(30)260..262(+)
Misc Feature(31)260..262(+)
Misc Feature(32)260..262(+)
Misc Feature(33)269..271(+)
Misc Feature(34)299..301(+)
Misc Feature(35)299..301(+)
Misc Feature(36)299..301(+)
Misc Feature(37)299..301(+)
Misc Feature(38)299..301(+)
Misc Feature(39)299..301(+)
Misc Feature(40)299..301(+)
Misc Feature(41)311..313(+)
Misc Feature(42)311..313(+)
Misc Feature(43)311..313(+)
Misc Feature(44)311..313(+)
Misc Feature(45)311..313(+)
Misc Feature(46)311..313(+)
Misc Feature(47)320..322(+)
Misc Feature(48)338..340(+)
Misc Feature(49)338..340(+)
Misc Feature(50)338..340(+)
Misc Feature(51)344..370(+)
Misc Feature(52)365..367(+)
Misc Feature(53)365..367(+)
Misc Feature(54)365..367(+)
Misc Feature(55)398..532(+)
Misc Feature(56)443..445(+)
Misc Feature(57)443..445(+)
Misc Feature(58)497..499(+)
Misc Feature(59)500..1312(+)
Misc Feature(60)500..1102(+)
Misc Feature(61)527..1066(+)
Misc Feature(62)539..910(+)
Misc Feature(63)548..1078(+)
Misc Feature(64)560..562(+)
Misc Feature(65)560..562(+)
Misc Feature(66)665..667(+)
Misc Feature(67)728..928(+)
Misc Feature(68)731..745(+)
Misc Feature(69)749..751(+)
Misc Feature(70)917..1042(+)
Misc Feature(71)923..946(+)
Misc Feature(72)968..1084(+)
Misc Feature(73)1007..1009(+)
Misc Feature(74)1019..1042(+)
Misc Feature(75)1052..1054(+)
Misc Feature(76)1076..1078(+)
Misc Feature(77)1100..1381(+)
Misc Feature(78)1115..1165(+)
Misc Feature(79)1115..1117(+)
Misc Feature(80)1115..1117(+)
Misc Feature(81)1145..1147(+)
Misc Feature(82)1145..1147(+)
Misc Feature(83)1145..1147(+)
Misc Feature(84)1145..1147(+)
Misc Feature(85)1145..1147(+)
Misc Feature(86)1145..1147(+)
Misc Feature(87)1145..1147(+)
Misc Feature(88)1157..1282(+)
Misc Feature(89)1157..1159(+)
Misc Feature(90)1160..1162(+)
Misc Feature(91)1160..1162(+)
Misc Feature(92)1160..1162(+)
Misc Feature(93)1163..1165(+)
Misc Feature(94)1163..1165(+)
Misc Feature(95)1172..1279(+)
Misc Feature(96)1175..1270(+)
Misc Feature(97)1217..1252(+)
Misc Feature(98)1253..1255(+)
Misc Feature(99)1271..1273(+)
Misc Feature(100)1277..1291(+)
Misc Feature(101)1298..1300(+)
Misc Feature(102)1304..1363(+)
Misc Feature(103)1310..1318(+)
Misc Feature(104)1310..1312(+)
Misc Feature(105)1310..1312(+)
Misc Feature(106)1310..1312(+)
Misc Feature(107)1310..1312(+)
Misc Feature(108)1310..1312(+)
Misc Feature(109)1313..1315(+)
Misc Feature(110)1316..1318(+)
Misc Feature(111)1316..1318(+)
Misc Feature(112)1316..1318(+)
Misc Feature(113)1316..1318(+)
Misc Feature(114)1316..1318(+)
Misc Feature(115)1319..1321(+)
Misc Feature(116)1319..1321(+)
Misc Feature(117)1319..1321(+)
Misc Feature(118)1319..1321(+)
Misc Feature(119)1319..1321(+)
Misc Feature(120)1319..1321(+)
Misc Feature(121)1334..1336(+)
Misc Feature(122)1343..1345(+)
Misc Feature(123)1343..1345(+)
Misc Feature(124)1343..1345(+)
Misc Feature(125)1343..1345(+)
Misc Feature(126)1346..1348(+)
Misc Feature(127)1346..1348(+)
Misc Feature(128)1346..1348(+)
Misc Feature(129)1346..1348(+)
Misc Feature(130)1346..1348(+)
Misc Feature(131)1346..1348(+)
Misc Feature(132)1358..1360(+)
Misc Feature(133)1358..1360(+)
Misc Feature(134)1358..1360(+)
Misc Feature(135)1376..1378(+)
Misc Feature(136)1376..1378(+)
Misc Feature(137)1376..1378(+)
Misc Feature(138)1376..1378(+)
Misc Feature(139)1376..1378(+)
Misc Feature(140)1376..1378(+)
Exon (1)1..174
Gene:TP53
Gene Synonym:
Exon (2)175..276
Gene:TP53
Gene Synonym:
Exon (3)277..298
Gene:TP53
Gene Synonym:
Exon (4)299..577
Gene:TP53
Gene Synonym:
Exon (5)578..761
Gene:TP53
Gene Synonym:
Exon (6)762..874
Gene:TP53
Gene Synonym:
Exon (7)875..984
Gene:TP53
Gene Synonym:
Exon (8)985..1121
Gene:TP53
Gene Synonym:
Exon (9)1122..1195
Gene:TP53
Gene Synonym:
Exon (10)1196..1302
Gene:TP53
Gene Synonym:
Exon (11)1303..2591
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000546
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000546

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAGGAGC CGCAGTCAGA TCCTAGCGTC GAGCCCCCTC TGAGTCAGGA AACATTTTCA 
GACCTATGGA AACTACTTCC TGAAAACAAC GTTCTGTCCC CCTTGCCGTC CCAAGCAATG
GATGATTTGA TGCTGTCCCC GGACGATATT GAACAATGGT TCACTGAAGA CCCAGGTCCA
GATGAAGCTC CCAGAATGCC AGAGGCTGCT CCCCCCGTGG CCCCTGCACC AGCAGCTCCT
ACACCGGCGG CCCCTGCACC AGCCCCCTCC TGGCCCCTGT CATCTTCTGT CCCTTCCCAG
AAAACCTACC AGGGCAGCTA CGGTTTCCGT CTGGGCTTCT TGCATTCTGG GACAGCCAAG
TCTGTGACTT GCACGTACTC CCCTGCCCTC AACAAGATGT TTTGCCAACT GGCCAAGACC
TGCCCTGTGC AGCTGTGGGT TGATTCCACA CCCCCGCCCG GCACCCGCGT CCGCGCCATG
GCCATCTACA AGCAGTCACA GCACATGACG GAGGTTGTGA GGCGCTGCCC CCACCATGAG
CGCTGCTCAG ATAGCGATGG TCTGGCCCCT CCTCAGCATC TTATCCGAGT GGAAGGAAAT
TTGCGTGTGG AGTATTTGGA TGACAGAAAC ACTTTTCGAC ATAGTGTGGT GGTGCCCTAT
GAGCCGCCTG AGGTTGGCTC TGACTGTACC ACCATCCACT ACAACTACAT GTGTAACAGT
TCCTGCATGG GCGGCATGAA CCGGAGGCCC ATCCTCACCA TCATCACACT GGAAGACTCC
AGTGGTAATC TACTGGGACG GAACAGCTTT GAGGTGCGTG TTTGTGCCTG TCCTGGGAGA
GACCGGCGCA CAGAGGAAGA GAATCTCCGC AAGAAAGGGG AGCCTCACCA CGAGCTGCCC
CCAGGGAGCA CTAAGCGAGC ACTGCCCAAC AACACCAGCT CCTCTCCCCA GCCAAAGAAG
AAACCACTGG ATGGAGAATA TTTCACCCTT CAGATCCGTG GGCGTGAGCG CTTCGAGATG
TTCCGAGAGC TGAATGAGGC CTTGGAACTC AAGGATGCCC AGGCTGGGAA GGAGCCAGGG
GGGAGCAGGG CTCACTCCAG CCACCTGAAG TCCAAAAAGG GTCAGTCTAC CTCCCGCCAT
AAAAAACTCA TGTTCAAGAC AGAAGGGCCT GACTCAGACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20059
Clone ID Related Accession (Same CDS sequence) NM_000546 , NM_001126112
Accession Version NM_001126112.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA453049.1, AB082923.1, X02469.1, AK223026.1 and DQ186650.1. This sequence is a reference standard in the RefSeqGene project. On Jan 7, 2012 this sequence version replaced gi:187830776. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. This variant can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (a, also known as p53alpha) results from translation initiation at the upstream start codon. Both variants 1 and 2 encode isoform a, which is the longest isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB082923.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAGGAGC CGCAGTCAGA TCCTAGCGTC GAGCCCCCTC TGAGTCAGGA AACATTTTCA 
GACCTATGGA AACTACTTCC TGAAAACAAC GTTCTGTCCC CCTTGCCGTC CCAAGCAATG
GATGATTTGA TGCTGTCCCC GGACGATATT GAACAATGGT TCACTGAAGA CCCAGGTCCA
GATGAAGCTC CCAGAATGCC AGAGGCTGCT CCCCCCGTGG CCCCTGCACC AGCAGCTCCT
ACACCGGCGG CCCCTGCACC AGCCCCCTCC TGGCCCCTGT CATCTTCTGT CCCTTCCCAG
AAAACCTACC AGGGCAGCTA CGGTTTCCGT CTGGGCTTCT TGCATTCTGG GACAGCCAAG
TCTGTGACTT GCACGTACTC CCCTGCCCTC AACAAGATGT TTTGCCAACT GGCCAAGACC
TGCCCTGTGC AGCTGTGGGT TGATTCCACA CCCCCGCCCG GCACCCGCGT CCGCGCCATG
GCCATCTACA AGCAGTCACA GCACATGACG GAGGTTGTGA GGCGCTGCCC CCACCATGAG
CGCTGCTCAG ATAGCGATGG TCTGGCCCCT CCTCAGCATC TTATCCGAGT GGAAGGAAAT
TTGCGTGTGG AGTATTTGGA TGACAGAAAC ACTTTTCGAC ATAGTGTGGT GGTGCCCTAT
GAGCCGCCTG AGGTTGGCTC TGACTGTACC ACCATCCACT ACAACTACAT GTGTAACAGT
TCCTGCATGG GCGGCATGAA CCGGAGGCCC ATCCTCACCA TCATCACACT GGAAGACTCC
AGTGGTAATC TACTGGGACG GAACAGCTTT GAGGTGCGTG TTTGTGCCTG TCCTGGGAGA
GACCGGCGCA CAGAGGAAGA GAATCTCCGC AAGAAAGGGG AGCCTCACCA CGAGCTGCCC
CCAGGGAGCA CTAAGCGAGC ACTGCCCAAC AACACCAGCT CCTCTCCCCA GCCAAAGAAG
AAACCACTGG ATGGAGAATA TTTCACCCTT CAGATCCGTG GGCGTGAGCG CTTCGAGATG
TTCCGAGAGC TGAATGAGGC CTTGGAACTC AAGGATGCCC AGGCTGGGAA GGAGCCAGGG
GGGAGCAGGG CTCACTCCAG CCACCTGAAG TCCAAAAAGG GTCAGTCTAC CTCCCGCCAT
AAAAAACTCA TGTTCAAGAC AGAAGGGCCT GACTCAGACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001119584.1
CDS200..1381
Misc Feature(1)95..97(+)
Misc Feature(2)200..448(+)
Misc Feature(3)200..331(+)
Misc Feature(4)212..286(+)
Misc Feature(5)224..226(+)
Misc Feature(6)242..244(+)
Misc Feature(7)248..274(+)
Misc Feature(8)251..253(+)
Misc Feature(9)257..259(+)
Misc Feature(10)296..298(+)
Misc Feature(11)308..310(+)
Misc Feature(12)317..319(+)
Misc Feature(13)335..337(+)
Misc Feature(14)341..367(+)
Misc Feature(15)362..364(+)
Misc Feature(16)395..529(+)
Misc Feature(17)497..1309(+)
Misc Feature(18)497..1099(+)
Misc Feature(19)524..1063(+)
Misc Feature(20)536..907(+)
Misc Feature(21)545..1075(+)
Misc Feature(22)557..559(+)
Misc Feature(23)557..559(+)
Misc Feature(24)725..925(+)
Misc Feature(25)728..742(+)
Misc Feature(26)746..748(+)
Misc Feature(27)914..1039(+)
Misc Feature(28)920..943(+)
Misc Feature(29)965..1081(+)
Misc Feature(30)1004..1006(+)
Misc Feature(31)1016..1039(+)
Misc Feature(32)1049..1051(+)
Misc Feature(33)1097..1378(+)
Misc Feature(34)1112..1162(+)
Misc Feature(35)1112..1114(+)
Misc Feature(36)1142..1144(+)
Misc Feature(37)1154..1279(+)
Misc Feature(38)1169..1276(+)
Misc Feature(39)1172..1267(+)
Misc Feature(40)1214..1249(+)
Misc Feature(41)1274..1288(+)
Misc Feature(42)1301..1360(+)
Misc Feature(43)1307..1315(+)
Misc Feature(44)1307..1309(+)
Misc Feature(45)1313..1315(+)
Misc Feature(46)1316..1318(+)
Misc Feature(47)1316..1318(+)
Misc Feature(48)1340..1342(+)
Misc Feature(49)1343..1345(+)
Misc Feature(50)1343..1345(+)
Misc Feature(51)1373..1375(+)
Exon (1)1..174
Gene:TP53
Gene Synonym:
Exon (2)175..273
Gene:TP53
Gene Synonym:
Exon (3)274..295
Gene:TP53
Gene Synonym:
Exon (4)296..574
Gene:TP53
Gene Synonym:
Exon (5)575..758
Gene:TP53
Gene Synonym:
Exon (6)759..871
Gene:TP53
Gene Synonym:
Exon (7)872..981
Gene:TP53
Gene Synonym:
Exon (8)982..1118
Gene:TP53
Gene Synonym:
Exon (9)1119..1192
Gene:TP53
Gene Synonym:
Exon (10)1193..1299
Gene:TP53
Gene Synonym:
Exon (11)1300..2588
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001126112
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001126112

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAGGAGC CGCAGTCAGA TCCTAGCGTC GAGCCCCCTC TGAGTCAGGA AACATTTTCA 
GACCTATGGA AACTACTTCC TGAAAACAAC GTTCTGTCCC CCTTGCCGTC CCAAGCAATG
GATGATTTGA TGCTGTCCCC GGACGATATT GAACAATGGT TCACTGAAGA CCCAGGTCCA
GATGAAGCTC CCAGAATGCC AGAGGCTGCT CCCCCCGTGG CCCCTGCACC AGCAGCTCCT
ACACCGGCGG CCCCTGCACC AGCCCCCTCC TGGCCCCTGT CATCTTCTGT CCCTTCCCAG
AAAACCTACC AGGGCAGCTA CGGTTTCCGT CTGGGCTTCT TGCATTCTGG GACAGCCAAG
TCTGTGACTT GCACGTACTC CCCTGCCCTC AACAAGATGT TTTGCCAACT GGCCAAGACC
TGCCCTGTGC AGCTGTGGGT TGATTCCACA CCCCCGCCCG GCACCCGCGT CCGCGCCATG
GCCATCTACA AGCAGTCACA GCACATGACG GAGGTTGTGA GGCGCTGCCC CCACCATGAG
CGCTGCTCAG ATAGCGATGG TCTGGCCCCT CCTCAGCATC TTATCCGAGT GGAAGGAAAT
TTGCGTGTGG AGTATTTGGA TGACAGAAAC ACTTTTCGAC ATAGTGTGGT GGTGCCCTAT
GAGCCGCCTG AGGTTGGCTC TGACTGTACC ACCATCCACT ACAACTACAT GTGTAACAGT
TCCTGCATGG GCGGCATGAA CCGGAGGCCC ATCCTCACCA TCATCACACT GGAAGACTCC
AGTGGTAATC TACTGGGACG GAACAGCTTT GAGGTGCGTG TTTGTGCCTG TCCTGGGAGA
GACCGGCGCA CAGAGGAAGA GAATCTCCGC AAGAAAGGGG AGCCTCACCA CGAGCTGCCC
CCAGGGAGCA CTAAGCGAGC ACTGCCCAAC AACACCAGCT CCTCTCCCCA GCCAAAGAAG
AAACCACTGG ATGGAGAATA TTTCACCCTT CAGATCCGTG GGCGTGAGCG CTTCGAGATG
TTCCGAGAGC TGAATGAGGC CTTGGAACTC AAGGATGCCC AGGCTGGGAA GGAGCCAGGG
GGGAGCAGGG CTCACTCCAG CCACCTGAAG TCCAAAAAGG GTCAGTCTAC CTCCCGCCAT
AAAAAACTCA TGTTCAAGAC AGAAGGGCCT GACTCAGACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20186
Clone ID Related Accession (Same CDS sequence) NM_001126113
Accession Version NM_001126113.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1041bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA453049.1, DQ186649.1, AK223026.1 and DQ186650.1. This sequence is a reference standard in the RefSeqGene project. On or before Aug 31, 2013 this sequence version replaced gi:530410603, gi:187830822. Transcript Variant: This variant (4) contains an additional exon in the 3' coding region, resulting in an alternate 3' coding region and 3' UTR, compared to variant 1. This variant can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (c, also known as p53gamma) results from translation initiation at the upstream start codon. It has a shorter and distinct C-terminus, compared to isoform a. CCDS Note: This CCDS ID represents the p53gamma isoform described in PMIDs: 16131611, 16601753 and 18289041. This variant is supported by the transcript DQ186649.1. It should be noted that this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because other similar isoforms derived from NMD transcripts were detected endogenously in PMID:16131611. In addition, experiments in PMID:17694537 indicate that this gene may be somewhat less sensitive to NMD. A contributing factor is the presence of a ubiquitination domain in the C-terminus of the full-length protein, which makes the protein less stable under non-stress conditions. C-terminally truncated proteins that lack the ubiquitination domain, such as this isoform, are therefore more stable. NMD inhibition experiments in the same study show that the majority of NMD transcripts do indeed undergo NMD, but some low level of NMD escape, combined with the increased stability of C-terminally truncated isoforms, allows for the expression of such isoforms. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## NMD candidate :: translation inferred ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: DQ186649.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2155371 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGGAGC CGCAGTCAGA TCCTAGCGTC GAGCCCCCTC TGAGTCAGGA AACATTTTCA 
GACCTATGGA AACTACTTCC TGAAAACAAC GTTCTGTCCC CCTTGCCGTC CCAAGCAATG
GATGATTTGA TGCTGTCCCC GGACGATATT GAACAATGGT TCACTGAAGA CCCAGGTCCA
GATGAAGCTC CCAGAATGCC AGAGGCTGCT CCCCCCGTGG CCCCTGCACC AGCAGCTCCT
ACACCGGCGG CCCCTGCACC AGCCCCCTCC TGGCCCCTGT CATCTTCTGT CCCTTCCCAG
AAAACCTACC AGGGCAGCTA CGGTTTCCGT CTGGGCTTCT TGCATTCTGG GACAGCCAAG
TCTGTGACTT GCACGTACTC CCCTGCCCTC AACAAGATGT TTTGCCAACT GGCCAAGACC
TGCCCTGTGC AGCTGTGGGT TGATTCCACA CCCCCGCCCG GCACCCGCGT CCGCGCCATG
GCCATCTACA AGCAGTCACA GCACATGACG GAGGTTGTGA GGCGCTGCCC CCACCATGAG
CGCTGCTCAG ATAGCGATGG TCTGGCCCCT CCTCAGCATC TTATCCGAGT GGAAGGAAAT
TTGCGTGTGG AGTATTTGGA TGACAGAAAC ACTTTTCGAC ATAGTGTGGT GGTGCCCTAT
GAGCCGCCTG AGGTTGGCTC TGACTGTACC ACCATCCACT ACAACTACAT GTGTAACAGT
TCCTGCATGG GCGGCATGAA CCGGAGGCCC ATCCTCACCA TCATCACACT GGAAGACTCC
AGTGGTAATC TACTGGGACG GAACAGCTTT GAGGTGCGTG TTTGTGCCTG TCCTGGGAGA
GACCGGCGCA CAGAGGAAGA GAATCTCCGC AAGAAAGGGG AGCCTCACCA CGAGCTGCCC
CCAGGGAGCA CTAAGCGAGC ACTGCCCAAC AACACCAGCT CCTCTCCCCA GCCAAAGAAG
AAACCACTGG ATGGAGAATA TTTCACCCTT CAGATGCTAC TTGACTTACG ATGGTGTTAC
TTCCTGATAA ACTCGTCGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001119585.1
CDS203..1243
Misc Feature(1)95..97(+)
Misc Feature(2)215..289(+)
Misc Feature(3)320..322(+)
Misc Feature(4)527..1066(+)
Misc Feature(5)728..928(+)
Misc Feature(6)731..745(+)
Misc Feature(7)917..1042(+)
Exon (1)1..174
Gene:TP53
Gene Synonym:
Exon (2)175..276
Gene:TP53
Gene Synonym:
Exon (3)277..298
Gene:TP53
Gene Synonym:
Exon (4)299..577
Gene:TP53
Gene Synonym:
Exon (5)578..761
Gene:TP53
Gene Synonym:
Exon (6)762..874
Gene:TP53
Gene Synonym:
Exon (7)875..984
Gene:TP53
Gene Synonym:
Exon (8)985..1121
Gene:TP53
Gene Synonym:
Exon (9)1122..1195
Gene:TP53
Gene Synonym:
Exon (10)1196..1255
Gene:TP53
Gene Synonym:
Exon (11)1256..1362
Gene:TP53
Gene Synonym:
Exon (12)1363..2651
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001126113
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001126113

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGGAGC CGCAGTCAGA TCCTAGCGTC GAGCCCCCTC TGAGTCAGGA AACATTTTCA 
GACCTATGGA AACTACTTCC TGAAAACAAC GTTCTGTCCC CCTTGCCGTC CCAAGCAATG
GATGATTTGA TGCTGTCCCC GGACGATATT GAACAATGGT TCACTGAAGA CCCAGGTCCA
GATGAAGCTC CCAGAATGCC AGAGGCTGCT CCCCCCGTGG CCCCTGCACC AGCAGCTCCT
ACACCGGCGG CCCCTGCACC AGCCCCCTCC TGGCCCCTGT CATCTTCTGT CCCTTCCCAG
AAAACCTACC AGGGCAGCTA CGGTTTCCGT CTGGGCTTCT TGCATTCTGG GACAGCCAAG
TCTGTGACTT GCACGTACTC CCCTGCCCTC AACAAGATGT TTTGCCAACT GGCCAAGACC
TGCCCTGTGC AGCTGTGGGT TGATTCCACA CCCCCGCCCG GCACCCGCGT CCGCGCCATG
GCCATCTACA AGCAGTCACA GCACATGACG GAGGTTGTGA GGCGCTGCCC CCACCATGAG
CGCTGCTCAG ATAGCGATGG TCTGGCCCCT CCTCAGCATC TTATCCGAGT GGAAGGAAAT
TTGCGTGTGG AGTATTTGGA TGACAGAAAC ACTTTTCGAC ATAGTGTGGT GGTGCCCTAT
GAGCCGCCTG AGGTTGGCTC TGACTGTACC ACCATCCACT ACAACTACAT GTGTAACAGT
TCCTGCATGG GCGGCATGAA CCGGAGGCCC ATCCTCACCA TCATCACACT GGAAGACTCC
AGTGGTAATC TACTGGGACG GAACAGCTTT GAGGTGCGTG TTTGTGCCTG TCCTGGGAGA
GACCGGCGCA CAGAGGAAGA GAATCTCCGC AAGAAAGGGG AGCCTCACCA CGAGCTGCCC
CCAGGGAGCA CTAAGCGAGC ACTGCCCAAC AACACCAGCT CCTCTCCCCA GCCAAAGAAG
AAACCACTGG ATGGAGAATA TTTCACCCTT CAGATGCTAC TTGACTTACG ATGGTGTTAC
TTCCTGATAA ACTCGTCGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20211
Clone ID Related Accession (Same CDS sequence) NM_001126114
Accession Version NM_001126114.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1026bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA453049.1, DQ186648.1, DQ186649.1, AK223026.1 and DQ186650.1. This sequence is a reference standard in the RefSeqGene project. On or before Aug 31, 2013 this sequence version replaced gi:530410604, gi:187830854. Transcript Variant: This variant (3) contains an additional exon in the 3' coding region, resulting in an alternate 3' coding region and 3' UTR, compared to variant 1. This variant can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (b, also known as p53beta) results from translation initiation at the upstream start codon. It has a shorter and distinct C-terminus, compared to isoform a. CCDS Note: This CCDS ID represents the p53beta isoform described in PMIDs: 16131611, 16601753 and 18289041. This variant is supported by the transcript DQ186648.1. It should be noted that this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID:16131611 using an antibody specific for the distinct C-terminus of this isoform. In addition, experiments in PMID:17694537 indicate that this gene may be somewhat less sensitive to NMD. A contributing factor is the presence of a ubiquitination domain in the C-terminus of the full-length protein, which makes the protein less stable under non-stress conditions. C-terminally truncated proteins that lack the ubiquitination domain, such as this isoform, are therefore more stable. NMD inhibition experiments in the same study show that the majority of NMD transcripts do indeed undergo NMD, but some low level of NMD escape, combined with the increased stability of C-terminally truncated isoforms, allows for the expression of such isoforms. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## NMD candidate :: PMID: 16131611, 16601753, 18289041 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: DQ186648.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGGAGC CGCAGTCAGA TCCTAGCGTC GAGCCCCCTC TGAGTCAGGA AACATTTTCA 
GACCTATGGA AACTACTTCC TGAAAACAAC GTTCTGTCCC CCTTGCCGTC CCAAGCAATG
GATGATTTGA TGCTGTCCCC GGACGATATT GAACAATGGT TCACTGAAGA CCCAGGTCCA
GATGAAGCTC CCAGAATGCC AGAGGCTGCT CCCCCCGTGG CCCCTGCACC AGCAGCTCCT
ACACCGGCGG CCCCTGCACC AGCCCCCTCC TGGCCCCTGT CATCTTCTGT CCCTTCCCAG
AAAACCTACC AGGGCAGCTA CGGTTTCCGT CTGGGCTTCT TGCATTCTGG GACAGCCAAG
TCTGTGACTT GCACGTACTC CCCTGCCCTC AACAAGATGT TTTGCCAACT GGCCAAGACC
TGCCCTGTGC AGCTGTGGGT TGATTCCACA CCCCCGCCCG GCACCCGCGT CCGCGCCATG
GCCATCTACA AGCAGTCACA GCACATGACG GAGGTTGTGA GGCGCTGCCC CCACCATGAG
CGCTGCTCAG ATAGCGATGG TCTGGCCCCT CCTCAGCATC TTATCCGAGT GGAAGGAAAT
TTGCGTGTGG AGTATTTGGA TGACAGAAAC ACTTTTCGAC ATAGTGTGGT GGTGCCCTAT
GAGCCGCCTG AGGTTGGCTC TGACTGTACC ACCATCCACT ACAACTACAT GTGTAACAGT
TCCTGCATGG GCGGCATGAA CCGGAGGCCC ATCCTCACCA TCATCACACT GGAAGACTCC
AGTGGTAATC TACTGGGACG GAACAGCTTT GAGGTGCGTG TTTGTGCCTG TCCTGGGAGA
GACCGGCGCA CAGAGGAAGA GAATCTCCGC AAGAAAGGGG AGCCTCACCA CGAGCTGCCC
CCAGGGAGCA CTAAGCGAGC ACTGCCCAAC AACACCAGCT CCTCTCCCCA GCCAAAGAAG
AAACCACTGG ATGGAGAATA TTTCACCCTT CAGGACCAGA CCAGCTTTCA AAAAGAAAAT
TGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001119586.1
CDS203..1228
Misc Feature(1)95..97(+)
Misc Feature(2)203..451(+)
Misc Feature(3)203..334(+)
Misc Feature(4)215..289(+)
Misc Feature(5)227..229(+)
Misc Feature(6)245..247(+)
Misc Feature(7)251..277(+)
Misc Feature(8)254..256(+)
Misc Feature(9)260..262(+)
Misc Feature(10)299..301(+)
Misc Feature(11)311..313(+)
Misc Feature(12)320..322(+)
Misc Feature(13)338..340(+)
Misc Feature(14)344..370(+)
Misc Feature(15)365..367(+)
Misc Feature(16)398..532(+)
Misc Feature(17)500..1102(+)
Misc Feature(18)527..1066(+)
Misc Feature(19)539..910(+)
Misc Feature(20)548..1078(+)
Misc Feature(21)560..562(+)
Misc Feature(22)560..562(+)
Misc Feature(23)728..928(+)
Misc Feature(24)731..745(+)
Misc Feature(25)749..751(+)
Misc Feature(26)917..1042(+)
Misc Feature(27)923..946(+)
Misc Feature(28)968..1084(+)
Misc Feature(29)1007..1009(+)
Misc Feature(30)1019..1042(+)
Misc Feature(31)1052..1054(+)
Misc Feature(32)1115..1165(+)
Misc Feature(33)1115..1117(+)
Misc Feature(34)1145..1147(+)
Exon (1)1..174
Gene:TP53
Gene Synonym:
Exon (2)175..276
Gene:TP53
Gene Synonym:
Exon (3)277..298
Gene:TP53
Gene Synonym:
Exon (4)299..577
Gene:TP53
Gene Synonym:
Exon (5)578..761
Gene:TP53
Gene Synonym:
Exon (6)762..874
Gene:TP53
Gene Synonym:
Exon (7)875..984
Gene:TP53
Gene Synonym:
Exon (8)985..1121
Gene:TP53
Gene Synonym:
Exon (9)1122..1195
Gene:TP53
Gene Synonym:
Exon (10)1196..1328
Gene:TP53
Gene Synonym:
Exon (11)1329..1435
Gene:TP53
Gene Synonym:
Exon (12)1436..2724
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001126114
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001126114

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGGAGC CGCAGTCAGA TCCTAGCGTC GAGCCCCCTC TGAGTCAGGA AACATTTTCA 
GACCTATGGA AACTACTTCC TGAAAACAAC GTTCTGTCCC CCTTGCCGTC CCAAGCAATG
GATGATTTGA TGCTGTCCCC GGACGATATT GAACAATGGT TCACTGAAGA CCCAGGTCCA
GATGAAGCTC CCAGAATGCC AGAGGCTGCT CCCCCCGTGG CCCCTGCACC AGCAGCTCCT
ACACCGGCGG CCCCTGCACC AGCCCCCTCC TGGCCCCTGT CATCTTCTGT CCCTTCCCAG
AAAACCTACC AGGGCAGCTA CGGTTTCCGT CTGGGCTTCT TGCATTCTGG GACAGCCAAG
TCTGTGACTT GCACGTACTC CCCTGCCCTC AACAAGATGT TTTGCCAACT GGCCAAGACC
TGCCCTGTGC AGCTGTGGGT TGATTCCACA CCCCCGCCCG GCACCCGCGT CCGCGCCATG
GCCATCTACA AGCAGTCACA GCACATGACG GAGGTTGTGA GGCGCTGCCC CCACCATGAG
CGCTGCTCAG ATAGCGATGG TCTGGCCCCT CCTCAGCATC TTATCCGAGT GGAAGGAAAT
TTGCGTGTGG AGTATTTGGA TGACAGAAAC ACTTTTCGAC ATAGTGTGGT GGTGCCCTAT
GAGCCGCCTG AGGTTGGCTC TGACTGTACC ACCATCCACT ACAACTACAT GTGTAACAGT
TCCTGCATGG GCGGCATGAA CCGGAGGCCC ATCCTCACCA TCATCACACT GGAAGACTCC
AGTGGTAATC TACTGGGACG GAACAGCTTT GAGGTGCGTG TTTGTGCCTG TCCTGGGAGA
GACCGGCGCA CAGAGGAAGA GAATCTCCGC AAGAAAGGGG AGCCTCACCA CGAGCTGCCC
CCAGGGAGCA CTAAGCGAGC ACTGCCCAAC AACACCAGCT CCTCTCCCCA GCCAAAGAAG
AAACCACTGG ATGGAGAATA TTTCACCCTT CAGGACCAGA CCAGCTTTCA AAAAGAAAAT
TGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16777
Clone ID Related Accession (Same CDS sequence) NM_001126118 , NM_001276760 , NM_001276761
Accession Version NM_001126118.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1065bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform g
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA453049.1, DC347346.1, X60016.1, DQ191317.1, AK223026.1 and DQ186650.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (8, also known as p53I2) differs in the 5' UTR, and uses an in-frame downstream start codon, compared to variant 1. The encoded isoform (g, also known as delta40p53alpha or deltaNp53) has a shorter N-terminus, compared to isoform a. This variant is supported by data in PMID:21112961. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JN900492.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGATGATT TGATGCTGTC CCCGGACGAT ATTGAACAAT GGTTCACTGA AGACCCAGGT 
CCAGATGAAG CTCCCAGAAT GCCAGAGGCT GCTCCCCCCG TGGCCCCTGC ACCAGCAGCT
CCTACACCGG CGGCCCCTGC ACCAGCCCCC TCCTGGCCCC TGTCATCTTC TGTCCCTTCC
CAGAAAACCT ACCAGGGCAG CTACGGTTTC CGTCTGGGCT TCTTGCATTC TGGGACAGCC
AAGTCTGTGA CTTGCACGTA CTCCCCTGCC CTCAACAAGA TGTTTTGCCA ACTGGCCAAG
ACCTGCCCTG TGCAGCTGTG GGTTGATTCC ACACCCCCGC CCGGCACCCG CGTCCGCGCC
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGATCC GTGGGCGTGA GCGCTTCGAG
ATGTTCCGAG AGCTGAATGA GGCCTTGGAA CTCAAGGATG CCCAGGCTGG GAAGGAGCCA
GGGGGGAGCA GGGCTCACTC CAGCCACCTG AAGTCCAAAA AGGGTCAGTC TACCTCCCGC
CATAAAAAAC TCATGTTCAA GACAGAAGGG CCTGACTCAG ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001119590.1
CDS437..1501
Misc Feature(1)278..280(+)
Misc Feature(2)644..1183(+)
Misc Feature(3)845..1045(+)
Misc Feature(4)848..862(+)
Misc Feature(5)1034..1159(+)
Misc Feature(6)1289..1396(+)
Exon (1)1..174
Gene:TP53
Gene Synonym:
Exon (2)175..415
Gene:TP53
Gene Synonym:
Exon (3)416..694
Gene:TP53
Gene Synonym:
Exon (4)695..878
Gene:TP53
Gene Synonym:
Exon (5)879..991
Gene:TP53
Gene Synonym:
Exon (6)992..1101
Gene:TP53
Gene Synonym:
Exon (7)1102..1238
Gene:TP53
Gene Synonym:
Exon (8)1239..1312
Gene:TP53
Gene Synonym:
Exon (9)1313..1419
Gene:TP53
Gene Synonym:
Exon (10)1420..2708
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001126118
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001126118

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGATGATT TGATGCTGTC CCCGGACGAT ATTGAACAAT GGTTCACTGA AGACCCAGGT 
CCAGATGAAG CTCCCAGAAT GCCAGAGGCT GCTCCCCCCG TGGCCCCTGC ACCAGCAGCT
CCTACACCGG CGGCCCCTGC ACCAGCCCCC TCCTGGCCCC TGTCATCTTC TGTCCCTTCC
CAGAAAACCT ACCAGGGCAG CTACGGTTTC CGTCTGGGCT TCTTGCATTC TGGGACAGCC
AAGTCTGTGA CTTGCACGTA CTCCCCTGCC CTCAACAAGA TGTTTTGCCA ACTGGCCAAG
ACCTGCCCTG TGCAGCTGTG GGTTGATTCC ACACCCCCGC CCGGCACCCG CGTCCGCGCC
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGATCC GTGGGCGTGA GCGCTTCGAG
ATGTTCCGAG AGCTGAATGA GGCCTTGGAA CTCAAGGATG CCCAGGCTGG GAAGGAGCCA
GGGGGGAGCA GGGCTCACTC CAGCCACCTG AAGTCCAAAA AGGGTCAGTC TACCTCCCGC
CATAAAAAAC TCATGTTCAA GACAGAAGGG CCTGACTCAG ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16674
Clone ID Related Accession (Same CDS sequence) NM_001276695
Accession Version NM_001276695.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 924bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform h
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA453049.1, DQ186649.1, AK223026.1 and DQ186650.1. Transcript Variant: This variant (4) contains an additional exon in the 3' coding region, resulting in an alternate 3' coding region and 3' UTR, compared to variant 1. This variant can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (h, also known as delta40p53gamma) results from translation initiation at the downstream start codon. It has a shorter N-terminus and a distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:12032546) NMD candidate :: translation inferred ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: DQ186649.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2155371 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGATGATT TGATGCTGTC CCCGGACGAT ATTGAACAAT GGTTCACTGA AGACCCAGGT 
CCAGATGAAG CTCCCAGAAT GCCAGAGGCT GCTCCCCCCG TGGCCCCTGC ACCAGCAGCT
CCTACACCGG CGGCCCCTGC ACCAGCCCCC TCCTGGCCCC TGTCATCTTC TGTCCCTTCC
CAGAAAACCT ACCAGGGCAG CTACGGTTTC CGTCTGGGCT TCTTGCATTC TGGGACAGCC
AAGTCTGTGA CTTGCACGTA CTCCCCTGCC CTCAACAAGA TGTTTTGCCA ACTGGCCAAG
ACCTGCCCTG TGCAGCTGTG GGTTGATTCC ACACCCCCGC CCGGCACCCG CGTCCGCGCC
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGATGC TACTTGACTT ACGATGGTGT
TACTTCCTGA TAAACTCGTC GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001263624.1
CDS320..1243
Misc Feature(1)95..97(+)
Misc Feature(2)203..205(+)
Misc Feature(3)527..1066(+)
Misc Feature(4)728..928(+)
Misc Feature(5)731..745(+)
Misc Feature(6)917..1042(+)
Exon (1)1..174
Gene:TP53
Gene Synonym:
Exon (2)175..276
Gene:TP53
Gene Synonym:
Exon (3)277..298
Gene:TP53
Gene Synonym:
Exon (4)299..577
Gene:TP53
Gene Synonym:
Exon (5)578..761
Gene:TP53
Gene Synonym:
Exon (6)762..874
Gene:TP53
Gene Synonym:
Exon (7)875..984
Gene:TP53
Gene Synonym:
Exon (8)985..1121
Gene:TP53
Gene Synonym:
Exon (9)1122..1195
Gene:TP53
Gene Synonym:
Exon (10)1196..1255
Gene:TP53
Gene Synonym:
Exon (11)1256..1362
Gene:TP53
Gene Synonym:
Exon (12)1363..2651
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001276695
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276695

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGATGATT TGATGCTGTC CCCGGACGAT ATTGAACAAT GGTTCACTGA AGACCCAGGT 
CCAGATGAAG CTCCCAGAAT GCCAGAGGCT GCTCCCCCCG TGGCCCCTGC ACCAGCAGCT
CCTACACCGG CGGCCCCTGC ACCAGCCCCC TCCTGGCCCC TGTCATCTTC TGTCCCTTCC
CAGAAAACCT ACCAGGGCAG CTACGGTTTC CGTCTGGGCT TCTTGCATTC TGGGACAGCC
AAGTCTGTGA CTTGCACGTA CTCCCCTGCC CTCAACAAGA TGTTTTGCCA ACTGGCCAAG
ACCTGCCCTG TGCAGCTGTG GGTTGATTCC ACACCCCCGC CCGGCACCCG CGTCCGCGCC
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGATGC TACTTGACTT ACGATGGTGT
TACTTCCTGA TAAACTCGTC GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16815
Clone ID Related Accession (Same CDS sequence) NM_001276696
Accession Version NM_001276696.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 909bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform i
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA453049.1, DQ186648.1, DQ186649.1, AK223026.1 and DQ186650.1. Transcript Variant: This variant (3) contains an additional exon in the 3' region, resulting in an alternate 3' coding region and 3' UTR, compared to variant 1. This variant can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (i, also known as delta40p53beta) results from translation initiation at the downstream start codon. It has a shorter N-terminus and a distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:12032546) NMD candidate :: translation inferred ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: DQ186648.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGATGATT TGATGCTGTC CCCGGACGAT ATTGAACAAT GGTTCACTGA AGACCCAGGT 
CCAGATGAAG CTCCCAGAAT GCCAGAGGCT GCTCCCCCCG TGGCCCCTGC ACCAGCAGCT
CCTACACCGG CGGCCCCTGC ACCAGCCCCC TCCTGGCCCC TGTCATCTTC TGTCCCTTCC
CAGAAAACCT ACCAGGGCAG CTACGGTTTC CGTCTGGGCT TCTTGCATTC TGGGACAGCC
AAGTCTGTGA CTTGCACGTA CTCCCCTGCC CTCAACAAGA TGTTTTGCCA ACTGGCCAAG
ACCTGCCCTG TGCAGCTGTG GGTTGATTCC ACACCCCCGC CCGGCACCCG CGTCCGCGCC
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGGACC AGACCAGCTT TCAAAAAGAA
AATTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001263625.1
CDS320..1228
Misc Feature(1)95..97(+)
Misc Feature(2)203..205(+)
Misc Feature(3)527..1066(+)
Misc Feature(4)728..928(+)
Misc Feature(5)731..745(+)
Misc Feature(6)917..1042(+)
Exon (1)1..174
Gene:TP53
Gene Synonym:
Exon (2)175..276
Gene:TP53
Gene Synonym:
Exon (3)277..298
Gene:TP53
Gene Synonym:
Exon (4)299..577
Gene:TP53
Gene Synonym:
Exon (5)578..761
Gene:TP53
Gene Synonym:
Exon (6)762..874
Gene:TP53
Gene Synonym:
Exon (7)875..984
Gene:TP53
Gene Synonym:
Exon (8)985..1121
Gene:TP53
Gene Synonym:
Exon (9)1122..1195
Gene:TP53
Gene Synonym:
Exon (10)1196..1328
Gene:TP53
Gene Synonym:
Exon (11)1329..1435
Gene:TP53
Gene Synonym:
Exon (12)1436..2724
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001276696
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276696

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGATGATT TGATGCTGTC CCCGGACGAT ATTGAACAAT GGTTCACTGA AGACCCAGGT 
CCAGATGAAG CTCCCAGAAT GCCAGAGGCT GCTCCCCCCG TGGCCCCTGC ACCAGCAGCT
CCTACACCGG CGGCCCCTGC ACCAGCCCCC TCCTGGCCCC TGTCATCTTC TGTCCCTTCC
CAGAAAACCT ACCAGGGCAG CTACGGTTTC CGTCTGGGCT TCTTGCATTC TGGGACAGCC
AAGTCTGTGA CTTGCACGTA CTCCCCTGCC CTCAACAAGA TGTTTTGCCA ACTGGCCAAG
ACCTGCCCTG TGCAGCTGTG GGTTGATTCC ACACCCCCGC CCGGCACCCG CGTCCGCGCC
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGGACC AGACCAGCTT TCAAAAAGAA
AATTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16777
Clone ID Related Accession (Same CDS sequence) NM_001126118 , NM_001276760 , NM_001276761
Accession Version NM_001276760.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1065bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform g
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA453049.1, X02469.1, AK223026.1 and DQ186650.1. Transcript Variant: This variant (1) can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (g, also known as delta40p53alpha or deltaNp53) results from translation initiation at the downstream start codon. It has a shorter N-terminus, compared to isoform a. Variants 1, 2, and 8 encode isoform g. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:12032546) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: DQ191317.1, DQ286964.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGATGATT TGATGCTGTC CCCGGACGAT ATTGAACAAT GGTTCACTGA AGACCCAGGT 
CCAGATGAAG CTCCCAGAAT GCCAGAGGCT GCTCCCCCCG TGGCCCCTGC ACCAGCAGCT
CCTACACCGG CGGCCCCTGC ACCAGCCCCC TCCTGGCCCC TGTCATCTTC TGTCCCTTCC
CAGAAAACCT ACCAGGGCAG CTACGGTTTC CGTCTGGGCT TCTTGCATTC TGGGACAGCC
AAGTCTGTGA CTTGCACGTA CTCCCCTGCC CTCAACAAGA TGTTTTGCCA ACTGGCCAAG
ACCTGCCCTG TGCAGCTGTG GGTTGATTCC ACACCCCCGC CCGGCACCCG CGTCCGCGCC
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGATCC GTGGGCGTGA GCGCTTCGAG
ATGTTCCGAG AGCTGAATGA GGCCTTGGAA CTCAAGGATG CCCAGGCTGG GAAGGAGCCA
GGGGGGAGCA GGGCTCACTC CAGCCACCTG AAGTCCAAAA AGGGTCAGTC TACCTCCCGC
CATAAAAAAC TCATGTTCAA GACAGAAGGG CCTGACTCAG ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001263689.1
CDS320..1384
Misc Feature(1)95..97(+)
Misc Feature(2)203..205(+)
Misc Feature(3)527..1066(+)
Misc Feature(4)728..928(+)
Misc Feature(5)731..745(+)
Misc Feature(6)917..1042(+)
Misc Feature(7)1172..1279(+)
Exon (1)1..174
Gene:TP53
Gene Synonym:
Exon (2)175..276
Gene:TP53
Gene Synonym:
Exon (3)277..298
Gene:TP53
Gene Synonym:
Exon (4)299..577
Gene:TP53
Gene Synonym:
Exon (5)578..761
Gene:TP53
Gene Synonym:
Exon (6)762..874
Gene:TP53
Gene Synonym:
Exon (7)875..984
Gene:TP53
Gene Synonym:
Exon (8)985..1121
Gene:TP53
Gene Synonym:
Exon (9)1122..1195
Gene:TP53
Gene Synonym:
Exon (10)1196..1302
Gene:TP53
Gene Synonym:
Exon (11)1303..2591
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001276760
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276760

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGATGATT TGATGCTGTC CCCGGACGAT ATTGAACAAT GGTTCACTGA AGACCCAGGT 
CCAGATGAAG CTCCCAGAAT GCCAGAGGCT GCTCCCCCCG TGGCCCCTGC ACCAGCAGCT
CCTACACCGG CGGCCCCTGC ACCAGCCCCC TCCTGGCCCC TGTCATCTTC TGTCCCTTCC
CAGAAAACCT ACCAGGGCAG CTACGGTTTC CGTCTGGGCT TCTTGCATTC TGGGACAGCC
AAGTCTGTGA CTTGCACGTA CTCCCCTGCC CTCAACAAGA TGTTTTGCCA ACTGGCCAAG
ACCTGCCCTG TGCAGCTGTG GGTTGATTCC ACACCCCCGC CCGGCACCCG CGTCCGCGCC
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGATCC GTGGGCGTGA GCGCTTCGAG
ATGTTCCGAG AGCTGAATGA GGCCTTGGAA CTCAAGGATG CCCAGGCTGG GAAGGAGCCA
GGGGGGAGCA GGGCTCACTC CAGCCACCTG AAGTCCAAAA AGGGTCAGTC TACCTCCCGC
CATAAAAAAC TCATGTTCAA GACAGAAGGG CCTGACTCAG ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16777
Clone ID Related Accession (Same CDS sequence) NM_001126118 , NM_001276760 , NM_001276761
Accession Version NM_001276761.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1065bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cellular tumor antigen p53 isoform g
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA453049.1, AB082923.1, X02469.1, AK223026.1 and DQ186650.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. This variant can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (g, also known as delta40p53alpha or deltaNp53) results from translation initiation at the downstream start codon. It has a shorter N-terminus, compared to isoform a. Variants 1, 2, and 8 encode isoform g. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:12032546) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AB082923.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGATGATT TGATGCTGTC CCCGGACGAT ATTGAACAAT GGTTCACTGA AGACCCAGGT 
CCAGATGAAG CTCCCAGAAT GCCAGAGGCT GCTCCCCCCG TGGCCCCTGC ACCAGCAGCT
CCTACACCGG CGGCCCCTGC ACCAGCCCCC TCCTGGCCCC TGTCATCTTC TGTCCCTTCC
CAGAAAACCT ACCAGGGCAG CTACGGTTTC CGTCTGGGCT TCTTGCATTC TGGGACAGCC
AAGTCTGTGA CTTGCACGTA CTCCCCTGCC CTCAACAAGA TGTTTTGCCA ACTGGCCAAG
ACCTGCCCTG TGCAGCTGTG GGTTGATTCC ACACCCCCGC CCGGCACCCG CGTCCGCGCC
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGATCC GTGGGCGTGA GCGCTTCGAG
ATGTTCCGAG AGCTGAATGA GGCCTTGGAA CTCAAGGATG CCCAGGCTGG GAAGGAGCCA
GGGGGGAGCA GGGCTCACTC CAGCCACCTG AAGTCCAAAA AGGGTCAGTC TACCTCCCGC
CATAAAAAAC TCATGTTCAA GACAGAAGGG CCTGACTCAG ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001263690.1
CDS317..1381
Misc Feature(1)95..97(+)
Misc Feature(2)200..202(+)
Misc Feature(3)524..1063(+)
Misc Feature(4)725..925(+)
Misc Feature(5)728..742(+)
Misc Feature(6)914..1039(+)
Misc Feature(7)1169..1276(+)
Exon (1)1..174
Gene:TP53
Gene Synonym:
Exon (2)175..273
Gene:TP53
Gene Synonym:
Exon (3)274..295
Gene:TP53
Gene Synonym:
Exon (4)296..574
Gene:TP53
Gene Synonym:
Exon (5)575..758
Gene:TP53
Gene Synonym:
Exon (6)759..871
Gene:TP53
Gene Synonym:
Exon (7)872..981
Gene:TP53
Gene Synonym:
Exon (8)982..1118
Gene:TP53
Gene Synonym:
Exon (9)1119..1192
Gene:TP53
Gene Synonym:
Exon (10)1193..1299
Gene:TP53
Gene Synonym:
Exon (11)1300..2588
Gene:TP53
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001276761
Organism Homo sapiens (human)
Definition Homo sapiens tumor protein p53 (TP53), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276761

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGATGATT TGATGCTGTC CCCGGACGAT ATTGAACAAT GGTTCACTGA AGACCCAGGT 
CCAGATGAAG CTCCCAGAAT GCCAGAGGCT GCTCCCCCCG TGGCCCCTGC ACCAGCAGCT
CCTACACCGG CGGCCCCTGC ACCAGCCCCC TCCTGGCCCC TGTCATCTTC TGTCCCTTCC
CAGAAAACCT ACCAGGGCAG CTACGGTTTC CGTCTGGGCT TCTTGCATTC TGGGACAGCC
AAGTCTGTGA CTTGCACGTA CTCCCCTGCC CTCAACAAGA TGTTTTGCCA ACTGGCCAAG
ACCTGCCCTG TGCAGCTGTG GGTTGATTCC ACACCCCCGC CCGGCACCCG CGTCCGCGCC
ATGGCCATCT ACAAGCAGTC ACAGCACATG ACGGAGGTTG TGAGGCGCTG CCCCCACCAT
GAGCGCTGCT CAGATAGCGA TGGTCTGGCC CCTCCTCAGC ATCTTATCCG AGTGGAAGGA
AATTTGCGTG TGGAGTATTT GGATGACAGA AACACTTTTC GACATAGTGT GGTGGTGCCC
TATGAGCCGC CTGAGGTTGG CTCTGACTGT ACCACCATCC ACTACAACTA CATGTGTAAC
AGTTCCTGCA TGGGCGGCAT GAACCGGAGG CCCATCCTCA CCATCATCAC ACTGGAAGAC
TCCAGTGGTA ATCTACTGGG ACGGAACAGC TTTGAGGTGC GTGTTTGTGC CTGTCCTGGG
AGAGACCGGC GCACAGAGGA AGAGAATCTC CGCAAGAAAG GGGAGCCTCA CCACGAGCTG
CCCCCAGGGA GCACTAAGCG AGCACTGCCC AACAACACCA GCTCCTCTCC CCAGCCAAAG
AAGAAACCAC TGGATGGAGA ATATTTCACC CTTCAGATCC GTGGGCGTGA GCGCTTCGAG
ATGTTCCGAG AGCTGAATGA GGCCTTGGAA CTCAAGGATG CCCAGGCTGG GAAGGAGCCA
GGGGGGAGCA GGGCTCACTC CAGCCACCTG AAGTCCAAAA AGGGTCAGTC TACCTCCCGC
CATAAAAAAC TCATGTTCAA GACAGAAGGG CCTGACTCAG ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

G-quadruplex structures in TP53 intron 3: role in alternative splicing and in production of p53 mRNA isoforms
Carcinogenesis 32 (3), 271-278 (2011)
Marcel V, Tran PL, Sagne C, Martel-Planche G, Vaslin L, Teulade-Fichou MP, Hall J, Mergny JL, Hainaut P and Van Dyck E.


book

Delta160p53 is a novel N-terminal p53 isoform encoded by Delta133p53 transcript
FEBS Lett. 584 (21), 4463-4468 (2010)
Marcel V, Perrier S, Aoubala M, Ageorges S, Groves MJ, Diot A, Fernandes K, Tauro S and Bourdon JC.


book

Does the nonsense-mediated mRNA decay mechanism prevent the synthesis of truncated BRCA1, CHK2, and p53 proteins?
Hum. Mutat. 29 (1), 65-73 (2008)
Anczukow O, Ware MD, Buisson M, Zetoune AB, Stoppa-Lyonnet D, Sinilnikova OM and Mazoyer S.


book

p53 Family isoforms
Curr Pharm Biotechnol 8 (6), 332-336 (2007)
Bourdon JC.


book

Li-Fraumeni Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Schneider,K., Zelley,K., Nichols,K.E. and Garber,J.


book

Characterization of the human p53 gene
Mol. Cell. Biol. 6 (5), 1379-1385 (1986)
Lamb,P. and Crawford,L.


book

Localization of gene for human p53 tumour antigen to band 17p13
Nature 320 (6057), 84-85 (1986)
Isobe,M., Emanuel,B.S., Givol,D., Oren,M. and Croce,C.M.


book

Transformation associated p53 protein is encoded by a gene on human chromosome 17
Somat. Cell Mol. Genet. 11 (5), 505-510 (1985)
Benchimol,S., Lamb,P., Crawford,L.V., Sheer,D., Shows,T.B., Bruns,G.A. and Peacock,J.


book

Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53
Mol. Cell. Biol. 5 (7), 1601-1610 (1985)
Harlow,E., Williamson,N.M., Ralston,R., Helfman,D.M. and Adams,T.E.


book

Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells
EMBO J. 4 (5), 1251-1255 (1985)
Zakut-Houri,R., Bienz-Tadmor,B., Givol,D. and Oren,M.


 
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