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TPM1 tropomyosin 1 (alpha) [Homo sapiens (human)]

Gene Symbol TPM1
Entrez Gene ID 7168
Full Name tropomyosin 1 (alpha)
Synonyms C15orf13, CMD1Y, CMH3, HTM-alpha, LVNC9, TMSA
General protein information
Preferred Names
tropomyosin alpha-1 chain
Names
tropomyosin alpha-1 chain
alpha-tropomyosin
sarcomeric tropomyosin kappa
cardiomyopathy, hypertrophic 3
tropomyosin 1 (alpha) isoform 1
tropomyosin 1 (alpha) isoform 2
tropomyosin 1 (alpha) isoform 3
tropomyosin 1 (alpha) isoform 4
tropomyosin 1 (alpha) isoform 5
tropomyosin 1 (alpha) isoform 6
tropomyosin 1 (alpha) isoform 7
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q22.1

Summary This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

191010

Disorder Html: Cardiomyopathy, familial hypertrophic, 3, 115196 (3); Cardiomyopathy,
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu48538 XM_005254638 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48539 XM_005254640 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48540 XM_005254639 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48541 XM_005254641 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48544 XM_006720667 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48545 XM_005254645 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48546 XM_005254646 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48547 XM_005254647 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48548 XM_005254648 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48550 XM_005254650 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48551 XM_005254651 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48552 XM_005254652 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48553 XM_005254653 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48554 XM_006720669 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48555 XM_005254637 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu16163 NM_001018008 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.12, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu21700 NM_001018006 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.7, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu21508 NM_001018004 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.6, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48549 NM_001301289 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.8, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu48542 NM_001301244 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu19397 NM_001018005 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu16327 NM_000366 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.5, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu20883 NM_001018020 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu16265 NM_001018007 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.4, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu48538D
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530406387. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005254695.1
CDS173..1036
Misc Feature(1)191..631(+)
Misc Feature(2)314..949(+)
Translation
Position Chain Variation Link
78 78 a, c dbSNP:559357765
79 79 c, g dbSNP:530410579
107 107 c, t dbSNP:193170738
108 108 c, t dbSNP:34325439
116 116 c, t dbSNP:548506915
120 120 g, t dbSNP:567007157
125 125 c, g, t dbSNP:747523720
126 126 a, c, g dbSNP:372760316
127 127 c, t dbSNP:374752523
129 129 c, t dbSNP:748349242
135 135 c, g dbSNP:770121340
136 136 c, t dbSNP:772865206
138 138 c, g dbSNP:749050935
144 144 c, t dbSNP:770633915
150 150 c, g dbSNP:774229154
151 151 a, t dbSNP:759494777
154 154 a, g dbSNP:766995600
166 166 a, c, t dbSNP:370871307
173 173 a, g dbSNP:367543668
174 174 -, t dbSNP:397516366
182 182 a, g dbSNP:730881148
190 190 a, g dbSNP:753446292
192 192 -, aga dbSNP:730881155
195 195 g, t dbSNP:397516364
197 197 a, c dbSNP:730881149
199 199 a, g dbSNP:397516365
203 203 c, t dbSNP:766883758
217 217 a, g, t dbSNP:199476301
218 218 c, g dbSNP:727504290
220 220 a, g dbSNP:755581369
225 225 a, c dbSNP:730881150
226 226 c, t dbSNP:781415770
230 230 a, g dbSNP:727504391
234 234 g, t dbSNP:730881151
236 236 a, g dbSNP:397516382
239 239 c, g dbSNP:199476302
249 249 a, g dbSNP:730880234
250 250 a, g dbSNP:777981114
254 254 a, c, g, t dbSNP:397516391
259 259 a, g dbSNP:530234301
263 263 a, g dbSNP:397516396
264 264 a, c dbSNP:749500508
265 265 a, c, g dbSNP:770661916
269 269 a, g dbSNP:397516397
276 276 c, g dbSNP:730881152
281 281 a, g dbSNP:199476303
289 289 -, g dbSNP:758264780
290 290 a, g, t dbSNP:104894501
310 310 a, g dbSNP:200509525
326 326 a, g dbSNP:730881127
327 327 g, t dbSNP:757460987
332 332 a, g dbSNP:104894505
335 335 a, g dbSNP:397516363
344 344 c, g dbSNP:199476304
346 346 c, t dbSNP:11558750
349 349 a, g dbSNP:149346709
352 352 c, t dbSNP:17850194
355 355 c, t dbSNP:758150342
356 356 c, g dbSNP:199476305
360 360 c, t dbSNP:199476306
361 361 c, t dbSNP:779737809
363 363 c, t dbSNP:730881142
381 381 a, c dbSNP:199476307
383 383 c, g dbSNP:730881143
420 420 a, c dbSNP:730881130
421 421 c, t dbSNP:200257214
422 422 a, g dbSNP:754664923
424 424 c, g, t dbSNP:369617788
425 425 a, g dbSNP:730881156
429 429 c, g dbSNP:757577112
436 436 a, g dbSNP:779411131
447 447 c, t dbSNP:199476310
448 448 c, g dbSNP:730881157
450 450 a, g dbSNP:746394586
454 454 a, g dbSNP:758503054
456 456 c, t dbSNP:104894504
460 460 a, g dbSNP:192883939
464 464 c, g dbSNP:730881131
465 465 a, g dbSNP:730881158
466 466 a, g dbSNP:747206706
473 473 c, g, t dbSNP:769069020
474 474 a, g dbSNP:747907799
477 477 a, c dbSNP:397516367
478 478 c, g dbSNP:769505504
480 480 a, g dbSNP:112125078
486 486 a, g dbSNP:773149185
493 493 a, c dbSNP:397516368
495 495 c, t dbSNP:762922595
505 505 a, g dbSNP:765843551
509 509 c, g dbSNP:397516369
513 513 a, g dbSNP:397516370
515 515 a, g dbSNP:727504313
526 526 g, t dbSNP:773916751
527 527 a, g dbSNP:759173967
553 553 a, g dbSNP:201211957
557 557 -, gt dbSNP:779940940
559 559 a, c, t dbSNP:769605281
561 561 c, t dbSNP:727503517
575 575 a, c dbSNP:11558749
582 582 a, g, t dbSNP:730881134
585 585 a, g dbSNP:786204410
588 588 a, t dbSNP:727504389
590 590 a, c dbSNP:730881135
595 595 c, g dbSNP:397516371
600 600 g, t dbSNP:730881136
616 616 c, g dbSNP:376518788
619 619 a, g dbSNP:774283931
625 625 a, c dbSNP:1071646
629 629 c, g dbSNP:397516372
630 630 a, g dbSNP:727505043
631 631 a, c dbSNP:771515165
646 646 c, t dbSNP:143922069
647 647 a, g dbSNP:397516373
651 651 a, g dbSNP:199476311
658 658 c, t dbSNP:11558747
679 679 a, g dbSNP:139663836
683 683 a, g dbSNP:774903903
685 685 c, g dbSNP:730881137
686 686 a, c dbSNP:760374266
687 687 c, t dbSNP:199476312
688 688 g, t dbSNP:730881138
694 694 c, t dbSNP:200173919
695 695 a, g dbSNP:104894503
704 704 c, t dbSNP:367606352
705 705 a, g dbSNP:397516375
711 711 a, g, t dbSNP:104894502
720 720 c, t dbSNP:397516376
721 721 c, g, t dbSNP:199476313
723 723 -, a dbSNP:730881153
726 726 g, t dbSNP:199476314
727 727 c, t dbSNP:752088217
731 731 c, g dbSNP:727504264
732 732 a, t dbSNP:786204411
740 740 c, t dbSNP:730881139
744 744 cc, tt dbSNP:730881154
744 744 c, t dbSNP:772303740
745 745 c, t dbSNP:780507307
746 746 a, g dbSNP:199476315
751 751 g, t dbSNP:1131002
757 757 a, g dbSNP:3218717
758 758 c, g dbSNP:1131003
764 764 a, g dbSNP:730881140
774 774 c, t dbSNP:730881141
775 775 a, c, g dbSNP:397516485
781 781 c, g dbSNP:397516486
792 792 -, t dbSNP:587777905
797 797 a, g dbSNP:730881159
799 799 g, t dbSNP:769210513
801 801 a, c dbSNP:777139450
802 802 a, g dbSNP:761816813
804 804 c, g dbSNP:397516487
806 806 -, ga dbSNP:199990219
807 807 a, t dbSNP:769951937
808 808 g, t dbSNP:17416757
816 816 c, t dbSNP:199476316
817 817 a, g dbSNP:773403386
827 827 a, g dbSNP:727503518
831 831 a, g dbSNP:763069444
835 835 c, t dbSNP:774859181
841 841 a, g dbSNP:573495443
845 845 a, g dbSNP:193922410
848 848 a, c dbSNP:397516383
849 849 a, g dbSNP:730881144
856 856 c, t dbSNP:397516384
859 859 c, t dbSNP:759362606
860 860 a, g dbSNP:199476317
864 864 a, g dbSNP:397516385
884 884 c, t dbSNP:397516386
885 885 a, g dbSNP:777716347
887 887 a, g dbSNP:199476318
897 897 c, t dbSNP:397516387
898 898 a, g dbSNP:200484871
906 906 c, t dbSNP:397516388
907 907 a, c, g dbSNP:771961466
912 912 a, c dbSNP:760516389
914 914 a, g dbSNP:199476319
915 915 a, c dbSNP:730881160
918 918 g, t dbSNP:786204412
925 925 a, g dbSNP:764036919
934 934 g, t dbSNP:727504354
940 940 a, g dbSNP:139650306
945 945 a, t dbSNP:759481997
950 950 -, tttc dbSNP:772897124
953 953 -, cttt dbSNP:786204415
954 954 g, t dbSNP:767681625
959 959 g, t dbSNP:752883071
966 966 c, t dbSNP:756393228
969 969 a, c dbSNP:146861835
978 978 c, t dbSNP:753572625
979 979 c, g, t dbSNP:757080224
981 981 c, t dbSNP:745841842
982 982 a, g dbSNP:778395303
984 984 c, t dbSNP:538518251
985 985 a, c, g dbSNP:201563826
996 996 c, g, t dbSNP:575130839
1001 1001 c, g dbSNP:780992464
1011 1011 c, t dbSNP:749572083
1012 1012 c, t dbSNP:771345862
1016 1016 a, g dbSNP:374459540
1023 1023 a, t dbSNP:760126994
1024 1024 a, g dbSNP:140658011
1029 1029 c, t dbSNP:775604378
1035 1035 c, g dbSNP:760757735
1040 1040 c, t dbSNP:764235066
1042 1042 a, g dbSNP:754091745
1047 1047 c, g, t dbSNP:150024529
1053 1053 c, t dbSNP:750300077
1076 1076 c, t dbSNP:540306699
1087 1087 c, t dbSNP:145513507
1093 1093 a, g dbSNP:148842623
1096 1096 g, t dbSNP:7668
1103 1103 a, g dbSNP:533687214
1110 1110 c, t dbSNP:775923793
1123 1123 a, g dbSNP:545752658
1132 1132 c, t dbSNP:764354563
1162 1162 c, t dbSNP:774595807
1196 1196 c, t dbSNP:563965885
1207 1207 c, t dbSNP:531178841

Target ORF information:

RefSeq Version XM_005254638
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005254638

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu48539D
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530406391. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005254697.1
CDS147..1010
Misc Feature(1)165..605(+)
Misc Feature(2)288..923(+)
Translation
Position Chain Variation Link
52 52 a, c dbSNP:559357765
53 53 c, g dbSNP:530410579
81 81 c, t dbSNP:193170738
82 82 c, t dbSNP:34325439
90 90 c, t dbSNP:548506915
94 94 g, t dbSNP:567007157
99 99 c, g, t dbSNP:747523720
100 100 a, c, g dbSNP:372760316
101 101 c, t dbSNP:374752523
103 103 c, t dbSNP:748349242
109 109 c, g dbSNP:770121340
110 110 c, t dbSNP:772865206
112 112 c, g dbSNP:749050935
118 118 c, t dbSNP:770633915
124 124 c, g dbSNP:774229154
125 125 a, t dbSNP:759494777
128 128 a, g dbSNP:766995600
140 140 a, c, t dbSNP:370871307
147 147 a, g dbSNP:367543668
148 148 -, t dbSNP:397516366
156 156 a, g dbSNP:730881148
164 164 a, g dbSNP:753446292
166 166 -, aga dbSNP:730881155
169 169 g, t dbSNP:397516364
171 171 a, c dbSNP:730881149
173 173 a, g dbSNP:397516365
177 177 c, t dbSNP:766883758
191 191 a, g, t dbSNP:199476301
192 192 c, g dbSNP:727504290
194 194 a, g dbSNP:755581369
199 199 a, c dbSNP:730881150
200 200 c, t dbSNP:781415770
204 204 a, g dbSNP:727504391
208 208 g, t dbSNP:730881151
210 210 a, g dbSNP:397516382
213 213 c, g dbSNP:199476302
223 223 a, g dbSNP:730880234
224 224 a, g dbSNP:777981114
228 228 a, c, g, t dbSNP:397516391
233 233 a, g dbSNP:530234301
237 237 a, g dbSNP:397516396
238 238 a, c dbSNP:749500508
239 239 a, c, g dbSNP:770661916
243 243 a, g dbSNP:397516397
250 250 c, g dbSNP:730881152
255 255 a, g dbSNP:199476303
263 263 c, g dbSNP:201720832
266 266 a, g dbSNP:397516491
275 275 c, t dbSNP:771867998
284 284 a, g dbSNP:786205439
307 307 a, t dbSNP:730881132
320 320 c, t dbSNP:74573041
321 321 c, t dbSNP:746952638
344 344 c, g dbSNP:730881126
366 366 a, g dbSNP:768164572
369 369 -, gaa dbSNP:752113248
371 371 -, tt dbSNP:760257899
378 378 c, g dbSNP:730881133
385 385 a, g dbSNP:527917980
394 394 a, c dbSNP:730881130
395 395 c, t dbSNP:200257214
396 396 a, g dbSNP:754664923
398 398 c, g, t dbSNP:369617788
399 399 a, g dbSNP:730881156
403 403 c, g dbSNP:757577112
410 410 a, g dbSNP:779411131
421 421 c, t dbSNP:199476310
422 422 c, g dbSNP:730881157
424 424 a, g dbSNP:746394586
428 428 a, g dbSNP:758503054
430 430 c, t dbSNP:104894504
434 434 a, g dbSNP:192883939
438 438 c, g dbSNP:730881131
439 439 a, g dbSNP:730881158
440 440 a, g dbSNP:747206706
447 447 c, g, t dbSNP:769069020
448 448 a, g dbSNP:747907799
451 451 a, c dbSNP:397516367
452 452 c, g dbSNP:769505504
454 454 a, g dbSNP:112125078
460 460 a, g dbSNP:773149185
467 467 a, c dbSNP:397516368
469 469 c, t dbSNP:762922595
479 479 a, g dbSNP:765843551
483 483 c, g dbSNP:397516369
487 487 a, g dbSNP:397516370
489 489 a, g dbSNP:727504313
500 500 g, t dbSNP:773916751
501 501 a, g dbSNP:759173967
527 527 a, g dbSNP:201211957
531 531 -, gt dbSNP:779940940
533 533 a, c, t dbSNP:769605281
535 535 c, t dbSNP:727503517
549 549 a, c dbSNP:11558749
556 556 a, g, t dbSNP:730881134
559 559 a, g dbSNP:786204410
562 562 a, t dbSNP:727504389
564 564 a, c dbSNP:730881135
569 569 c, g dbSNP:397516371
574 574 g, t dbSNP:730881136
590 590 c, g dbSNP:376518788
593 593 a, g dbSNP:774283931
599 599 a, c dbSNP:1071646
603 603 c, g dbSNP:397516372
604 604 a, g dbSNP:727505043
605 605 a, c dbSNP:771515165
620 620 c, t dbSNP:143922069
621 621 a, g dbSNP:397516373
625 625 a, g dbSNP:199476311
632 632 c, t dbSNP:11558747
653 653 a, g dbSNP:139663836
657 657 a, g dbSNP:774903903
659 659 c, g dbSNP:730881137
660 660 a, c dbSNP:760374266
661 661 c, t dbSNP:199476312
662 662 g, t dbSNP:730881138
668 668 c, t dbSNP:200173919
669 669 a, g dbSNP:104894503
678 678 c, t dbSNP:367606352
679 679 a, g dbSNP:397516375
685 685 a, g, t dbSNP:104894502
694 694 c, t dbSNP:397516376
695 695 c, g, t dbSNP:199476313
697 697 -, a dbSNP:730881153
700 700 g, t dbSNP:199476314
701 701 c, t dbSNP:752088217
705 705 c, g dbSNP:727504264
706 706 a, t dbSNP:786204411
714 714 c, t dbSNP:730881139
718 718 cc, tt dbSNP:730881154
718 718 c, t dbSNP:772303740
719 719 c, t dbSNP:780507307
720 720 a, g dbSNP:199476315
725 725 g, t dbSNP:1131002
731 731 a, g dbSNP:3218717
732 732 c, g dbSNP:1131003
738 738 a, g dbSNP:730881140
748 748 c, t dbSNP:730881141
749 749 a, c, g dbSNP:397516485
755 755 c, g dbSNP:397516486
766 766 -, t dbSNP:587777905
771 771 a, g dbSNP:730881159
773 773 g, t dbSNP:769210513
775 775 a, c dbSNP:777139450
776 776 a, g dbSNP:761816813
778 778 c, g dbSNP:397516487
780 780 -, ga dbSNP:199990219
781 781 a, t dbSNP:769951937
782 782 g, t dbSNP:17416757
790 790 c, t dbSNP:199476316
791 791 a, g dbSNP:773403386
801 801 a, g dbSNP:727503518
805 805 a, g dbSNP:763069444
809 809 c, t dbSNP:774859181
815 815 a, g dbSNP:573495443
819 819 a, g dbSNP:193922410
822 822 a, c dbSNP:397516383
823 823 a, g dbSNP:730881144
830 830 c, t dbSNP:397516384
833 833 c, t dbSNP:759362606
834 834 a, g dbSNP:199476317
838 838 a, g dbSNP:397516385
858 858 c, t dbSNP:397516386
859 859 a, g dbSNP:777716347
861 861 a, g dbSNP:199476318
871 871 c, t dbSNP:397516387
872 872 a, g dbSNP:200484871
880 880 c, t dbSNP:397516388
881 881 a, c, g dbSNP:771961466
886 886 a, c dbSNP:760516389
888 888 a, g dbSNP:199476319
889 889 a, c dbSNP:730881160
892 892 g, t dbSNP:786204412
899 899 a, g dbSNP:764036919
908 908 g, t dbSNP:727504354
914 914 a, g dbSNP:139650306
919 919 a, t dbSNP:759481997
924 924 -, tttc dbSNP:772897124
927 927 -, cttt dbSNP:786204415
928 928 g, t dbSNP:767681625
933 933 g, t dbSNP:752883071
940 940 c, t dbSNP:756393228
943 943 a, c dbSNP:146861835
952 952 c, t dbSNP:753572625
953 953 c, g, t dbSNP:757080224
955 955 c, t dbSNP:745841842
956 956 a, g dbSNP:778395303
958 958 c, t dbSNP:538518251
959 959 a, c, g dbSNP:201563826
970 970 c, g, t dbSNP:575130839
975 975 c, g dbSNP:780992464
985 985 c, t dbSNP:749572083
986 986 c, t dbSNP:771345862
990 990 a, g dbSNP:374459540
997 997 a, t dbSNP:760126994
998 998 a, g dbSNP:140658011
1003 1003 c, t dbSNP:775604378
1009 1009 c, g dbSNP:760757735
1014 1014 c, t dbSNP:764235066
1016 1016 a, g dbSNP:754091745
1021 1021 c, g, t dbSNP:150024529
1027 1027 c, t dbSNP:750300077
1050 1050 c, t dbSNP:540306699
1061 1061 c, t dbSNP:145513507
1067 1067 a, g dbSNP:148842623
1070 1070 g, t dbSNP:7668
1077 1077 a, g dbSNP:533687214
1084 1084 c, t dbSNP:775923793
1097 1097 a, g dbSNP:545752658
1106 1106 c, t dbSNP:764354563
1136 1136 c, t dbSNP:774595807
1170 1170 c, t dbSNP:563965885
1181 1181 c, t dbSNP:531178841

Target ORF information:

RefSeq Version XM_005254640
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005254640

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu48540D
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530406389. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005254696.1
CDS173..1036
Misc Feature(1)191..631(+)
Misc Feature(2)314..949(+)
Translation
Position Chain Variation Link
78 78 a, c dbSNP:559357765
79 79 c, g dbSNP:530410579
107 107 c, t dbSNP:193170738
108 108 c, t dbSNP:34325439
116 116 c, t dbSNP:548506915
120 120 g, t dbSNP:567007157
125 125 c, g, t dbSNP:747523720
126 126 a, c, g dbSNP:372760316
127 127 c, t dbSNP:374752523
129 129 c, t dbSNP:748349242
135 135 c, g dbSNP:770121340
136 136 c, t dbSNP:772865206
138 138 c, g dbSNP:749050935
144 144 c, t dbSNP:770633915
150 150 c, g dbSNP:774229154
151 151 a, t dbSNP:759494777
154 154 a, g dbSNP:766995600
166 166 a, c, t dbSNP:370871307
173 173 a, g dbSNP:367543668
174 174 -, t dbSNP:397516366
182 182 a, g dbSNP:730881148
190 190 a, g dbSNP:753446292
192 192 -, aga dbSNP:730881155
195 195 g, t dbSNP:397516364
197 197 a, c dbSNP:730881149
199 199 a, g dbSNP:397516365
203 203 c, t dbSNP:766883758
217 217 a, g, t dbSNP:199476301
218 218 c, g dbSNP:727504290
220 220 a, g dbSNP:755581369
225 225 a, c dbSNP:730881150
226 226 c, t dbSNP:781415770
230 230 a, g dbSNP:727504391
234 234 g, t dbSNP:730881151
236 236 a, g dbSNP:397516382
239 239 c, g dbSNP:199476302
249 249 a, g dbSNP:730880234
250 250 a, g dbSNP:777981114
254 254 a, c, g, t dbSNP:397516391
259 259 a, g dbSNP:530234301
263 263 a, g dbSNP:397516396
264 264 a, c dbSNP:749500508
265 265 a, c, g dbSNP:770661916
269 269 a, g dbSNP:397516397
276 276 c, g dbSNP:730881152
281 281 a, g dbSNP:199476303
289 289 -, g dbSNP:758264780
290 290 a, g, t dbSNP:104894501
310 310 a, g dbSNP:200509525
326 326 a, g dbSNP:730881127
327 327 g, t dbSNP:757460987
332 332 a, g dbSNP:104894505
335 335 a, g dbSNP:397516363
344 344 c, g dbSNP:199476304
346 346 c, t dbSNP:11558750
349 349 a, g dbSNP:149346709
352 352 c, t dbSNP:17850194
355 355 c, t dbSNP:758150342
356 356 c, g dbSNP:199476305
360 360 c, t dbSNP:199476306
361 361 c, t dbSNP:779737809
363 363 c, t dbSNP:730881142
381 381 a, c dbSNP:199476307
383 383 c, g dbSNP:730881143
420 420 a, c dbSNP:730881130
421 421 c, t dbSNP:200257214
422 422 a, g dbSNP:754664923
424 424 c, g, t dbSNP:369617788
425 425 a, g dbSNP:730881156
429 429 c, g dbSNP:757577112
436 436 a, g dbSNP:779411131
447 447 c, t dbSNP:199476310
448 448 c, g dbSNP:730881157
450 450 a, g dbSNP:746394586
454 454 a, g dbSNP:758503054
456 456 c, t dbSNP:104894504
460 460 a, g dbSNP:192883939
464 464 c, g dbSNP:730881131
465 465 a, g dbSNP:730881158
466 466 a, g dbSNP:747206706
473 473 c, g, t dbSNP:769069020
474 474 a, g dbSNP:747907799
477 477 a, c dbSNP:397516367
478 478 c, g dbSNP:769505504
480 480 a, g dbSNP:112125078
486 486 a, g dbSNP:773149185
493 493 a, c dbSNP:397516368
495 495 c, t dbSNP:762922595
505 505 a, g dbSNP:765843551
509 509 c, g dbSNP:397516369
513 513 a, g dbSNP:397516370
515 515 a, g dbSNP:727504313
526 526 g, t dbSNP:773916751
527 527 a, g dbSNP:759173967
553 553 a, g dbSNP:201211957
557 557 -, gt dbSNP:779940940
559 559 a, c, t dbSNP:769605281
561 561 c, t dbSNP:727503517
575 575 a, c dbSNP:11558749
582 582 a, g, t dbSNP:730881134
585 585 a, g dbSNP:786204410
588 588 a, t dbSNP:727504389
590 590 a, c dbSNP:730881135
595 595 c, g dbSNP:397516371
600 600 g, t dbSNP:730881136
616 616 c, g dbSNP:376518788
619 619 a, g dbSNP:774283931
625 625 a, c dbSNP:1071646
629 629 c, g dbSNP:397516372
630 630 a, g dbSNP:727505043
631 631 a, c dbSNP:771515165
646 646 c, t dbSNP:143922069
647 647 a, g dbSNP:397516373
651 651 a, g dbSNP:199476311
658 658 c, t dbSNP:11558747
679 679 a, g dbSNP:139663836
683 683 a, g dbSNP:774903903
685 685 c, g dbSNP:730881137
686 686 a, c dbSNP:760374266
687 687 c, t dbSNP:199476312
688 688 g, t dbSNP:730881138
694 694 c, t dbSNP:200173919
695 695 a, g dbSNP:104894503
704 704 c, t dbSNP:367606352
705 705 a, g dbSNP:397516375
711 711 a, g, t dbSNP:104894502
720 720 c, t dbSNP:397516376
721 721 c, g, t dbSNP:199476313
723 723 -, a dbSNP:730881153
726 726 g, t dbSNP:199476314
727 727 c, t dbSNP:752088217
731 731 c, g dbSNP:727504264
732 732 a, t dbSNP:786204411
743 743 a, c, t dbSNP:201813515
744 744 a, g dbSNP:141757680
760 760 a, g dbSNP:765013065
766 766 a, t dbSNP:200794206
780 780 c, g dbSNP:766058944
782 782 c, t dbSNP:779791290
789 789 c, t dbSNP:747001359
790 790 a, g dbSNP:144700226
816 816 c, t dbSNP:199476316
817 817 a, g dbSNP:773403386
827 827 a, g dbSNP:727503518
831 831 a, g dbSNP:763069444
835 835 c, t dbSNP:774859181
841 841 a, g dbSNP:573495443
845 845 a, g dbSNP:193922410
848 848 a, c dbSNP:397516383
849 849 a, g dbSNP:730881144
856 856 c, t dbSNP:397516384
859 859 c, t dbSNP:759362606
860 860 a, g dbSNP:199476317
864 864 a, g dbSNP:397516385
884 884 c, t dbSNP:397516386
885 885 a, g dbSNP:777716347
887 887 a, g dbSNP:199476318
897 897 c, t dbSNP:397516387
898 898 a, g dbSNP:200484871
906 906 c, t dbSNP:397516388
907 907 a, c, g dbSNP:771961466
912 912 a, c dbSNP:760516389
914 914 a, g dbSNP:199476319
915 915 a, c dbSNP:730881160
918 918 g, t dbSNP:786204412
925 925 a, g dbSNP:764036919
934 934 g, t dbSNP:727504354
940 940 a, g dbSNP:139650306
945 945 a, t dbSNP:759481997
950 950 -, tttc dbSNP:772897124
953 953 -, cttt dbSNP:786204415
954 954 g, t dbSNP:767681625
959 959 g, t dbSNP:752883071
966 966 c, t dbSNP:756393228
969 969 a, c dbSNP:146861835
978 978 c, t dbSNP:753572625
979 979 c, g, t dbSNP:757080224
981 981 c, t dbSNP:745841842
982 982 a, g dbSNP:778395303
984 984 c, t dbSNP:538518251
985 985 a, c, g dbSNP:201563826
996 996 c, g, t dbSNP:575130839
1001 1001 c, g dbSNP:780992464
1011 1011 c, t dbSNP:749572083
1012 1012 c, t dbSNP:771345862
1016 1016 a, g dbSNP:374459540
1023 1023 a, t dbSNP:760126994
1024 1024 a, g dbSNP:140658011
1029 1029 c, t dbSNP:775604378
1035 1035 c, g dbSNP:760757735
1040 1040 c, t dbSNP:764235066
1042 1042 a, g dbSNP:754091745
1047 1047 c, g, t dbSNP:150024529
1053 1053 c, t dbSNP:750300077
1076 1076 c, t dbSNP:540306699
1087 1087 c, t dbSNP:145513507
1093 1093 a, g dbSNP:148842623
1096 1096 g, t dbSNP:7668
1103 1103 a, g dbSNP:533687214
1110 1110 c, t dbSNP:775923793
1123 1123 a, g dbSNP:545752658
1132 1132 c, t dbSNP:764354563
1162 1162 c, t dbSNP:774595807
1196 1196 c, t dbSNP:563965885
1207 1207 c, t dbSNP:531178841

Target ORF information:

RefSeq Version XM_005254639
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005254639

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu48541D
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530406393. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005254698.1
CDS147..1010
Misc Feature(1)165..605(+)
Misc Feature(2)288..923(+)
Translation
Position Chain Variation Link
52 52 a, c dbSNP:559357765
53 53 c, g dbSNP:530410579
81 81 c, t dbSNP:193170738
82 82 c, t dbSNP:34325439
90 90 c, t dbSNP:548506915
94 94 g, t dbSNP:567007157
99 99 c, g, t dbSNP:747523720
100 100 a, c, g dbSNP:372760316
101 101 c, t dbSNP:374752523
103 103 c, t dbSNP:748349242
109 109 c, g dbSNP:770121340
110 110 c, t dbSNP:772865206
112 112 c, g dbSNP:749050935
118 118 c, t dbSNP:770633915
124 124 c, g dbSNP:774229154
125 125 a, t dbSNP:759494777
128 128 a, g dbSNP:766995600
140 140 a, c, t dbSNP:370871307
147 147 a, g dbSNP:367543668
148 148 -, t dbSNP:397516366
156 156 a, g dbSNP:730881148
164 164 a, g dbSNP:753446292
166 166 -, aga dbSNP:730881155
169 169 g, t dbSNP:397516364
171 171 a, c dbSNP:730881149
173 173 a, g dbSNP:397516365
177 177 c, t dbSNP:766883758
191 191 a, g, t dbSNP:199476301
192 192 c, g dbSNP:727504290
194 194 a, g dbSNP:755581369
199 199 a, c dbSNP:730881150
200 200 c, t dbSNP:781415770
204 204 a, g dbSNP:727504391
208 208 g, t dbSNP:730881151
210 210 a, g dbSNP:397516382
213 213 c, g dbSNP:199476302
223 223 a, g dbSNP:730880234
224 224 a, g dbSNP:777981114
228 228 a, c, g, t dbSNP:397516391
233 233 a, g dbSNP:530234301
237 237 a, g dbSNP:397516396
238 238 a, c dbSNP:749500508
239 239 a, c, g dbSNP:770661916
243 243 a, g dbSNP:397516397
250 250 c, g dbSNP:730881152
255 255 a, g dbSNP:199476303
263 263 c, g dbSNP:201720832
266 266 a, g dbSNP:397516491
275 275 c, t dbSNP:771867998
284 284 a, g dbSNP:786205439
307 307 a, t dbSNP:730881132
320 320 c, t dbSNP:74573041
321 321 c, t dbSNP:746952638
344 344 c, g dbSNP:730881126
366 366 a, g dbSNP:768164572
369 369 -, gaa dbSNP:752113248
371 371 -, tt dbSNP:760257899
378 378 c, g dbSNP:730881133
385 385 a, g dbSNP:527917980
394 394 a, c dbSNP:730881130
395 395 c, t dbSNP:200257214
396 396 a, g dbSNP:754664923
398 398 c, g, t dbSNP:369617788
399 399 a, g dbSNP:730881156
403 403 c, g dbSNP:757577112
410 410 a, g dbSNP:779411131
421 421 c, t dbSNP:199476310
422 422 c, g dbSNP:730881157
424 424 a, g dbSNP:746394586
428 428 a, g dbSNP:758503054
430 430 c, t dbSNP:104894504
434 434 a, g dbSNP:192883939
438 438 c, g dbSNP:730881131
439 439 a, g dbSNP:730881158
440 440 a, g dbSNP:747206706
447 447 c, g, t dbSNP:769069020
448 448 a, g dbSNP:747907799
451 451 a, c dbSNP:397516367
452 452 c, g dbSNP:769505504
454 454 a, g dbSNP:112125078
460 460 a, g dbSNP:773149185
467 467 a, c dbSNP:397516368
469 469 c, t dbSNP:762922595
479 479 a, g dbSNP:765843551
483 483 c, g dbSNP:397516369
487 487 a, g dbSNP:397516370
489 489 a, g dbSNP:727504313
500 500 g, t dbSNP:773916751
501 501 a, g dbSNP:759173967
527 527 a, g dbSNP:201211957
531 531 -, gt dbSNP:779940940
533 533 a, c, t dbSNP:769605281
535 535 c, t dbSNP:727503517
549 549 a, c dbSNP:11558749
556 556 a, g, t dbSNP:730881134
559 559 a, g dbSNP:786204410
562 562 a, t dbSNP:727504389
564 564 a, c dbSNP:730881135
569 569 c, g dbSNP:397516371
574 574 g, t dbSNP:730881136
590 590 c, g dbSNP:376518788
593 593 a, g dbSNP:774283931
599 599 a, c dbSNP:1071646
603 603 c, g dbSNP:397516372
604 604 a, g dbSNP:727505043
605 605 a, c dbSNP:771515165
620 620 c, t dbSNP:143922069
621 621 a, g dbSNP:397516373
625 625 a, g dbSNP:199476311
632 632 c, t dbSNP:11558747
653 653 a, g dbSNP:139663836
657 657 a, g dbSNP:774903903
659 659 c, g dbSNP:730881137
660 660 a, c dbSNP:760374266
661 661 c, t dbSNP:199476312
662 662 g, t dbSNP:730881138
668 668 c, t dbSNP:200173919
669 669 a, g dbSNP:104894503
678 678 c, t dbSNP:367606352
679 679 a, g dbSNP:397516375
685 685 a, g, t dbSNP:104894502
694 694 c, t dbSNP:397516376
695 695 c, g, t dbSNP:199476313
697 697 -, a dbSNP:730881153
700 700 g, t dbSNP:199476314
701 701 c, t dbSNP:752088217
705 705 c, g dbSNP:727504264
706 706 a, t dbSNP:786204411
717 717 a, c, t dbSNP:201813515
718 718 a, g dbSNP:141757680
734 734 a, g dbSNP:765013065
740 740 a, t dbSNP:200794206
754 754 c, g dbSNP:766058944
756 756 c, t dbSNP:779791290
763 763 c, t dbSNP:747001359
764 764 a, g dbSNP:144700226
790 790 c, t dbSNP:199476316
791 791 a, g dbSNP:773403386
801 801 a, g dbSNP:727503518
805 805 a, g dbSNP:763069444
809 809 c, t dbSNP:774859181
815 815 a, g dbSNP:573495443
819 819 a, g dbSNP:193922410
822 822 a, c dbSNP:397516383
823 823 a, g dbSNP:730881144
830 830 c, t dbSNP:397516384
833 833 c, t dbSNP:759362606
834 834 a, g dbSNP:199476317
838 838 a, g dbSNP:397516385
858 858 c, t dbSNP:397516386
859 859 a, g dbSNP:777716347
861 861 a, g dbSNP:199476318
871 871 c, t dbSNP:397516387
872 872 a, g dbSNP:200484871
880 880 c, t dbSNP:397516388
881 881 a, c, g dbSNP:771961466
886 886 a, c dbSNP:760516389
888 888 a, g dbSNP:199476319
889 889 a, c dbSNP:730881160
892 892 g, t dbSNP:786204412
899 899 a, g dbSNP:764036919
908 908 g, t dbSNP:727504354
914 914 a, g dbSNP:139650306
919 919 a, t dbSNP:759481997
924 924 -, tttc dbSNP:772897124
927 927 -, cttt dbSNP:786204415
928 928 g, t dbSNP:767681625
933 933 g, t dbSNP:752883071
940 940 c, t dbSNP:756393228
943 943 a, c dbSNP:146861835
952 952 c, t dbSNP:753572625
953 953 c, g, t dbSNP:757080224
955 955 c, t dbSNP:745841842
956 956 a, g dbSNP:778395303
958 958 c, t dbSNP:538518251
959 959 a, c, g dbSNP:201563826
970 970 c, g, t dbSNP:575130839
975 975 c, g dbSNP:780992464
985 985 c, t dbSNP:749572083
986 986 c, t dbSNP:771345862
990 990 a, g dbSNP:374459540
997 997 a, t dbSNP:760126994
998 998 a, g dbSNP:140658011
1003 1003 c, t dbSNP:775604378
1009 1009 c, g dbSNP:760757735
1014 1014 c, t dbSNP:764235066
1016 1016 a, g dbSNP:754091745
1021 1021 c, g, t dbSNP:150024529
1027 1027 c, t dbSNP:750300077
1050 1050 c, t dbSNP:540306699
1061 1061 c, t dbSNP:145513507
1067 1067 a, g dbSNP:148842623
1070 1070 g, t dbSNP:7668
1077 1077 a, g dbSNP:533687214
1084 1084 c, t dbSNP:775923793
1097 1097 a, g dbSNP:545752658
1106 1106 c, t dbSNP:764354563
1136 1136 c, t dbSNP:774595807
1170 1170 c, t dbSNP:563965885
1181 1181 c, t dbSNP:531178841

Target ORF information:

RefSeq Version XM_005254641
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005254641

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu48544D
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578827354. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TACGCTCAGA AACTGAAGTA CAAAGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_006720730.1
CDS187..1041
Misc Feature(1)205..645(+)
Misc Feature(2)328..1038(+)
Translation
Position Chain Variation Link
1 1 a, g dbSNP:541046450
92 92 a, c dbSNP:559357765
93 93 c, g dbSNP:530410579
121 121 c, t dbSNP:193170738
122 122 c, t dbSNP:34325439
130 130 c, t dbSNP:548506915
134 134 g, t dbSNP:567007157
139 139 c, g, t dbSNP:747523720
140 140 a, c, g dbSNP:372760316
141 141 c, t dbSNP:374752523
143 143 c, t dbSNP:748349242
149 149 c, g dbSNP:770121340
150 150 c, t dbSNP:772865206
152 152 c, g dbSNP:749050935
158 158 c, t dbSNP:770633915
164 164 c, g dbSNP:774229154
165 165 a, t dbSNP:759494777
168 168 a, g dbSNP:766995600
180 180 a, c, t dbSNP:370871307
187 187 a, g dbSNP:367543668
188 188 -, t dbSNP:397516366
196 196 a, g dbSNP:730881148
204 204 a, g dbSNP:753446292
206 206 -, aga dbSNP:730881155
209 209 g, t dbSNP:397516364
211 211 a, c dbSNP:730881149
213 213 a, g dbSNP:397516365
217 217 c, t dbSNP:766883758
231 231 a, g, t dbSNP:199476301
232 232 c, g dbSNP:727504290
234 234 a, g dbSNP:755581369
239 239 a, c dbSNP:730881150
240 240 c, t dbSNP:781415770
244 244 a, g dbSNP:727504391
248 248 g, t dbSNP:730881151
250 250 a, g dbSNP:397516382
253 253 c, g dbSNP:199476302
263 263 a, g dbSNP:730880234
264 264 a, g dbSNP:777981114
268 268 a, c, g, t dbSNP:397516391
273 273 a, g dbSNP:530234301
277 277 a, g dbSNP:397516396
278 278 a, c dbSNP:749500508
279 279 a, c, g dbSNP:770661916
283 283 a, g dbSNP:397516397
290 290 c, g dbSNP:730881152
295 295 a, g dbSNP:199476303
303 303 -, g dbSNP:758264780
304 304 a, g, t dbSNP:104894501
324 324 a, g dbSNP:200509525
340 340 a, g dbSNP:730881127
341 341 g, t dbSNP:757460987
346 346 a, g dbSNP:104894505
349 349 a, g dbSNP:397516363
358 358 c, g dbSNP:199476304
360 360 c, t dbSNP:11558750
363 363 a, g dbSNP:149346709
366 366 c, t dbSNP:17850194
369 369 c, t dbSNP:758150342
370 370 c, g dbSNP:199476305
374 374 c, t dbSNP:199476306
375 375 c, t dbSNP:779737809
377 377 c, t dbSNP:730881142
395 395 a, c dbSNP:199476307
397 397 c, g dbSNP:730881143
434 434 a, c dbSNP:730881130
435 435 c, t dbSNP:200257214
436 436 a, g dbSNP:754664923
438 438 c, g, t dbSNP:369617788
439 439 a, g dbSNP:730881156
443 443 c, g dbSNP:757577112
450 450 a, g dbSNP:779411131
461 461 c, t dbSNP:199476310
462 462 c, g dbSNP:730881157
464 464 a, g dbSNP:746394586
468 468 a, g dbSNP:758503054
470 470 c, t dbSNP:104894504
474 474 a, g dbSNP:192883939
478 478 c, g dbSNP:730881131
479 479 a, g dbSNP:730881158
480 480 a, g dbSNP:747206706
487 487 c, g, t dbSNP:769069020
488 488 a, g dbSNP:747907799
491 491 a, c dbSNP:397516367
492 492 c, g dbSNP:769505504
494 494 a, g dbSNP:112125078
500 500 a, g dbSNP:773149185
507 507 a, c dbSNP:397516368
509 509 c, t dbSNP:762922595
519 519 a, g dbSNP:765843551
523 523 c, g dbSNP:397516369
527 527 a, g dbSNP:397516370
529 529 a, g dbSNP:727504313
540 540 g, t dbSNP:773916751
541 541 a, g dbSNP:759173967
567 567 a, g dbSNP:201211957
571 571 -, gt dbSNP:779940940
573 573 a, c, t dbSNP:769605281
575 575 c, t dbSNP:727503517
589 589 a, c dbSNP:11558749
596 596 a, g, t dbSNP:730881134
599 599 a, g dbSNP:786204410
602 602 a, t dbSNP:727504389
604 604 a, c dbSNP:730881135
609 609 c, g dbSNP:397516371
614 614 g, t dbSNP:730881136
630 630 c, g dbSNP:376518788
633 633 a, g dbSNP:774283931
639 639 a, c dbSNP:1071646
643 643 c, g dbSNP:397516372
644 644 a, g dbSNP:727505043
645 645 a, c dbSNP:771515165
660 660 c, t dbSNP:143922069
661 661 a, g dbSNP:397516373
665 665 a, g dbSNP:199476311
672 672 c, t dbSNP:11558747
693 693 a, g dbSNP:139663836
697 697 a, g dbSNP:774903903
699 699 c, g dbSNP:730881137
700 700 a, c dbSNP:760374266
701 701 c, t dbSNP:199476312
702 702 g, t dbSNP:730881138
708 708 c, t dbSNP:200173919
709 709 a, g dbSNP:104894503
718 718 c, t dbSNP:367606352
719 719 a, g dbSNP:397516375
725 725 a, g, t dbSNP:104894502
734 734 c, t dbSNP:397516376
735 735 c, g, t dbSNP:199476313
737 737 -, a dbSNP:730881153
740 740 g, t dbSNP:199476314
741 741 c, t dbSNP:752088217
745 745 c, g dbSNP:727504264
746 746 a, t dbSNP:786204411
757 757 a, c, t dbSNP:201813515
758 758 a, g dbSNP:141757680
774 774 a, g dbSNP:765013065
780 780 a, t dbSNP:200794206
794 794 c, g dbSNP:766058944
796 796 c, t dbSNP:779791290
803 803 c, t dbSNP:747001359
804 804 a, g dbSNP:144700226
830 830 c, t dbSNP:199476316
831 831 a, g dbSNP:773403386
841 841 a, g dbSNP:727503518
845 845 a, g dbSNP:763069444
849 849 c, t dbSNP:774859181
855 855 a, g dbSNP:573495443
859 859 a, g dbSNP:193922410
862 862 a, c dbSNP:397516383
863 863 a, g dbSNP:730881144
870 870 c, t dbSNP:397516384
873 873 c, t dbSNP:759362606
874 874 a, g dbSNP:199476317
878 878 a, g dbSNP:397516385
898 898 c, t dbSNP:397516386
899 899 a, g dbSNP:777716347
901 901 a, g dbSNP:199476318
911 911 c, t dbSNP:397516387
912 912 a, g dbSNP:200484871
920 920 c, t dbSNP:397516388
921 921 a, c, g dbSNP:771961466
926 926 a, c dbSNP:760516389
928 928 a, g dbSNP:199476319
929 929 a, c dbSNP:730881160
932 932 g, t dbSNP:786204412
939 939 a, g dbSNP:764036919
948 948 g, t dbSNP:727504354
954 954 a, g dbSNP:139650306
960 960 c, t dbSNP:762282433
961 961 a, c, g dbSNP:397516389
969 969 c, t dbSNP:751001221
970 970 a, g dbSNP:758506771
973 973 c, g dbSNP:730881147
976 976 a, g dbSNP:397516390
983 983 a, g dbSNP:371934474
993 993 c, t dbSNP:751778912
1000 1000 a, g dbSNP:755226348
1001 1001 a, c, g dbSNP:727503519
1014 1014 c, t dbSNP:368813394
1015 1015 a, g dbSNP:149659674
1016 1016 c, g, t dbSNP:199476320
1018 1018 c, t dbSNP:730881161
1021 1021 a, c dbSNP:397516392
1023 1023 c, t dbSNP:397516393
1024 1024 a, g dbSNP:777635889
1026 1026 c, t dbSNP:749271066
1027 1027 a, g dbSNP:397516394
1028 1028 c, t dbSNP:199476321
1031 1031 c, g dbSNP:397516395
1034 1034 -, c dbSNP:775857037
1036 1036 a, g dbSNP:199476322
1037 1037 c, t dbSNP:745520822
1038 1038 a, t dbSNP:759481997
1043 1043 -, tttc dbSNP:772897124
1046 1046 -, cttt dbSNP:786204415
1047 1047 g, t dbSNP:767681625
1052 1052 g, t dbSNP:752883071
1059 1059 c, t dbSNP:756393228
1062 1062 a, c dbSNP:146861835
1071 1071 c, t dbSNP:753572625
1072 1072 c, g, t dbSNP:757080224
1074 1074 c, t dbSNP:745841842
1075 1075 a, g dbSNP:778395303
1077 1077 c, t dbSNP:538518251
1078 1078 a, c, g dbSNP:201563826
1089 1089 c, g, t dbSNP:575130839
1094 1094 c, g dbSNP:780992464
1104 1104 c, t dbSNP:749572083
1105 1105 c, t dbSNP:771345862
1109 1109 a, g dbSNP:374459540
1116 1116 a, t dbSNP:760126994
1117 1117 a, g dbSNP:140658011
1122 1122 c, t dbSNP:775604378
1128 1128 c, g dbSNP:760757735
1133 1133 c, t dbSNP:764235066
1135 1135 a, g dbSNP:754091745
1140 1140 c, g, t dbSNP:150024529
1146 1146 c, t dbSNP:750300077
1169 1169 c, t dbSNP:540306699
1180 1180 c, t dbSNP:145513507
1186 1186 a, g dbSNP:148842623
1189 1189 g, t dbSNP:7668
1196 1196 a, g dbSNP:533687214
1203 1203 c, t dbSNP:775923793
1216 1216 a, g dbSNP:545752658
1225 1225 c, t dbSNP:764354563
1255 1255 c, t dbSNP:774595807
1289 1289 c, t dbSNP:563965885
1300 1300 c, t dbSNP:531178841

Target ORF information:

RefSeq Version XM_006720667
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_006720667

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TACGCTCAGA AACTGAAGTA CAAAGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu48545D
Sequence Information ORF Nucleotide Sequence (Length: 846bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATCAACTC
TACCAGCAAC TTGAGCAAAA TCGCCGCCTC ACTAATGAAC TAAAGCTGGC CCTGAATGAG
GATTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005254702.1
CDS173..1018
Misc Feature(1)191..631(+)
Misc Feature(2)314..1012(+)
Translation
Position Chain Variation Link
78 78 a, c dbSNP:559357765
79 79 c, g dbSNP:530410579
107 107 c, t dbSNP:193170738
108 108 c, t dbSNP:34325439
116 116 c, t dbSNP:548506915
120 120 g, t dbSNP:567007157
125 125 c, g, t dbSNP:747523720
126 126 a, c, g dbSNP:372760316
127 127 c, t dbSNP:374752523
129 129 c, t dbSNP:748349242
135 135 c, g dbSNP:770121340
136 136 c, t dbSNP:772865206
138 138 c, g dbSNP:749050935
144 144 c, t dbSNP:770633915
150 150 c, g dbSNP:774229154
151 151 a, t dbSNP:759494777
154 154 a, g dbSNP:766995600
166 166 a, c, t dbSNP:370871307
173 173 a, g dbSNP:367543668
174 174 -, t dbSNP:397516366
182 182 a, g dbSNP:730881148
190 190 a, g dbSNP:753446292
192 192 -, aga dbSNP:730881155
195 195 g, t dbSNP:397516364
197 197 a, c dbSNP:730881149
199 199 a, g dbSNP:397516365
203 203 c, t dbSNP:766883758
217 217 a, g, t dbSNP:199476301
218 218 c, g dbSNP:727504290
220 220 a, g dbSNP:755581369
225 225 a, c dbSNP:730881150
226 226 c, t dbSNP:781415770
230 230 a, g dbSNP:727504391
234 234 g, t dbSNP:730881151
236 236 a, g dbSNP:397516382
239 239 c, g dbSNP:199476302
249 249 a, g dbSNP:730880234
250 250 a, g dbSNP:777981114
254 254 a, c, g, t dbSNP:397516391
259 259 a, g dbSNP:530234301
263 263 a, g dbSNP:397516396
264 264 a, c dbSNP:749500508
265 265 a, c, g dbSNP:770661916
269 269 a, g dbSNP:397516397
276 276 c, g dbSNP:730881152
281 281 a, g dbSNP:199476303
289 289 -, g dbSNP:758264780
290 290 a, g, t dbSNP:104894501
310 310 a, g dbSNP:200509525
326 326 a, g dbSNP:730881127
327 327 g, t dbSNP:757460987
332 332 a, g dbSNP:104894505
335 335 a, g dbSNP:397516363
344 344 c, g dbSNP:199476304
346 346 c, t dbSNP:11558750
349 349 a, g dbSNP:149346709
352 352 c, t dbSNP:17850194
355 355 c, t dbSNP:758150342
356 356 c, g dbSNP:199476305
360 360 c, t dbSNP:199476306
361 361 c, t dbSNP:779737809
363 363 c, t dbSNP:730881142
381 381 a, c dbSNP:199476307
383 383 c, g dbSNP:730881143
420 420 a, c dbSNP:730881130
421 421 c, t dbSNP:200257214
422 422 a, g dbSNP:754664923
424 424 c, g, t dbSNP:369617788
425 425 a, g dbSNP:730881156
429 429 c, g dbSNP:757577112
436 436 a, g dbSNP:779411131
447 447 c, t dbSNP:199476310
448 448 c, g dbSNP:730881157
450 450 a, g dbSNP:746394586
454 454 a, g dbSNP:758503054
456 456 c, t dbSNP:104894504
460 460 a, g dbSNP:192883939
464 464 c, g dbSNP:730881131
465 465 a, g dbSNP:730881158
466 466 a, g dbSNP:747206706
473 473 c, g, t dbSNP:769069020
474 474 a, g dbSNP:747907799
477 477 a, c dbSNP:397516367
478 478 c, g dbSNP:769505504
480 480 a, g dbSNP:112125078
486 486 a, g dbSNP:773149185
493 493 a, c dbSNP:397516368
495 495 c, t dbSNP:762922595
505 505 a, g dbSNP:765843551
509 509 c, g dbSNP:397516369
513 513 a, g dbSNP:397516370
515 515 a, g dbSNP:727504313
526 526 g, t dbSNP:773916751
527 527 a, g dbSNP:759173967
553 553 a, g dbSNP:201211957
557 557 -, gt dbSNP:779940940
559 559 a, c, t dbSNP:769605281
561 561 c, t dbSNP:727503517
575 575 a, c dbSNP:11558749
582 582 a, g, t dbSNP:730881134
585 585 a, g dbSNP:786204410
588 588 a, t dbSNP:727504389
590 590 a, c dbSNP:730881135
595 595 c, g dbSNP:397516371
600 600 g, t dbSNP:730881136
616 616 c, g dbSNP:376518788
619 619 a, g dbSNP:774283931
625 625 a, c dbSNP:1071646
629 629 c, g dbSNP:397516372
630 630 a, g dbSNP:727505043
631 631 a, c dbSNP:771515165
646 646 c, t dbSNP:143922069
647 647 a, g dbSNP:397516373
651 651 a, g dbSNP:199476311
658 658 c, t dbSNP:11558747
679 679 a, g dbSNP:139663836
683 683 a, g dbSNP:774903903
685 685 c, g dbSNP:730881137
686 686 a, c dbSNP:760374266
687 687 c, t dbSNP:199476312
688 688 g, t dbSNP:730881138
694 694 c, t dbSNP:200173919
695 695 a, g dbSNP:104894503
704 704 c, t dbSNP:367606352
705 705 a, g dbSNP:397516375
711 711 a, g, t dbSNP:104894502
720 720 c, t dbSNP:397516376
721 721 c, g, t dbSNP:199476313
723 723 -, a dbSNP:730881153
726 726 g, t dbSNP:199476314
727 727 c, t dbSNP:752088217
731 731 c, g dbSNP:727504264
732 732 a, t dbSNP:786204411
740 740 c, t dbSNP:730881139
744 744 cc, tt dbSNP:730881154
744 744 c, t dbSNP:772303740
745 745 c, t dbSNP:780507307
746 746 a, g dbSNP:199476315
751 751 g, t dbSNP:1131002
757 757 a, g dbSNP:3218717
758 758 c, g dbSNP:1131003
764 764 a, g dbSNP:730881140
774 774 c, t dbSNP:730881141
775 775 a, c, g dbSNP:397516485
781 781 c, g dbSNP:397516486
792 792 -, t dbSNP:587777905
797 797 a, g dbSNP:730881159
799 799 g, t dbSNP:769210513
801 801 a, c dbSNP:777139450
802 802 a, g dbSNP:761816813
804 804 c, g dbSNP:397516487
806 806 -, ga dbSNP:199990219
807 807 a, t dbSNP:769951937
808 808 g, t dbSNP:17416757
816 816 c, t dbSNP:199476316
817 817 a, g dbSNP:773403386
827 827 a, g dbSNP:727503518
831 831 a, g dbSNP:763069444
835 835 c, t dbSNP:774859181
841 841 a, g dbSNP:573495443
845 845 a, g dbSNP:193922410
848 848 a, c dbSNP:397516383
849 849 a, g dbSNP:730881144
856 856 c, t dbSNP:397516384
859 859 c, t dbSNP:759362606
860 860 a, g dbSNP:199476317
864 864 a, g dbSNP:397516385
884 884 c, t dbSNP:397516386
885 885 a, g dbSNP:777716347
887 887 a, g dbSNP:199476318
897 897 c, t dbSNP:397516387
898 898 a, g dbSNP:200484871
906 906 c, t dbSNP:397516388
907 907 a, c, g dbSNP:771961466
912 912 a, c dbSNP:760516389
914 914 a, g dbSNP:199476319
915 915 a, c dbSNP:730881160
918 918 g, t dbSNP:786204412
925 925 a, g dbSNP:764036919
934 934 g, t dbSNP:727504354
940 940 a, g dbSNP:139650306
950 950 c, g dbSNP:567912676
955 955 c, t dbSNP:369307852
959 959 a, c dbSNP:771167283
965 965 a, g dbSNP:779398864
966 966 a, g dbSNP:746345875
968 968 c, t dbSNP:772509023
975 975 a, g dbSNP:730881128
977 977 c, t dbSNP:760706706
978 978 a, g dbSNP:144451403
982 982 a, c, t dbSNP:776697819
987 987 a, g dbSNP:764939532
991 991 a, g dbSNP:187444963
998 998 c, g, t dbSNP:762673645
1010 1010 -, g dbSNP:762701496
1012 1012 a, g dbSNP:772871597
1013 1013 a, g dbSNP:754511941
1017 1017 a, c dbSNP:139159081
1023 1023 a, c dbSNP:369978558
1025 1025 c, g dbSNP:755864491
1027 1027 a, g dbSNP:373028120
1037 1037 -, tt dbSNP:766260876
1037 1037 g, t dbSNP:531582147
1040 1040 c, g dbSNP:758785276
1042 1042 a, c dbSNP:780497547
1044 1044 a, g dbSNP:747553234
1048 1048 g, t dbSNP:141403692
1049 1049 c, g dbSNP:568658056
1054 1054 c, t dbSNP:375617240
1055 1055 a, c, g dbSNP:73440433
1062 1062 a, c dbSNP:547633494
1064 1064 a, g dbSNP:762800883
1071 1071 -, g dbSNP:751435902
1102 1102 c, t dbSNP:114073739
1132 1132 a, g dbSNP:191417491
1135 1135 c, g dbSNP:764526574
1143 1143 a, g dbSNP:557865070
1168 1168 a, g dbSNP:569700841
1169 1169 c, t dbSNP:536630665
1184 1184 a, g dbSNP:751816418
1203 1203 a, g dbSNP:555677406
1246 1246 a, t dbSNP:573805348
1250 1250 a, g dbSNP:757526232
1255 1255 g, t dbSNP:2414814
1288 1288 a, t dbSNP:570213136
1299 1299 a, g dbSNP:552724410
1363 1363 -, tg, tgtg dbSNP:151128731
1365 1365 a, g dbSNP:182044936
1366 1366 -, gtgt dbSNP:61703644
1367 1367 a, g dbSNP:79345411
1369 1369 a, g dbSNP:76275714
1376 1376 -, atac dbSNP:554415917
1379 1379 -, tatatatg, tg dbSNP:775945347
1379 1379 a, g dbSNP:200580287
1381 1381 a, g dbSNP:77963487
1382 1382 -, atatatatatatatatat, atatatatatatgtgt, atatatatatgtgtgt dbSNP:58377224
1383 1383 a, g dbSNP:143569714
1385 1385 a, g dbSNP:531642589
1399 1399 a, g dbSNP:200938707
1401 1401 -, tgta, tgtgtgta dbSNP:147930353
1401 1401 a, g dbSNP:113519012
1402 1402 -, gtatat, gtgtgt dbSNP:61140537
1403 1403 a, g dbSNP:12903543
1408 1408 -, atat dbSNP:60393555
1432 1432 a, g dbSNP:759514819
1502 1502 -, a dbSNP:34533927
1516 1516 a, g dbSNP:16946436
1517 1517 g, t dbSNP:746060317
1560 1560 c, g dbSNP:547990922
1564 1564 c, t dbSNP:557704771
1656 1656 a, g dbSNP:775653798
1665 1665 a, g dbSNP:756237380
1672 1672 a, g dbSNP:192584695
1723 1723 c, t dbSNP:148028089
1737 1737 a, g dbSNP:79829181
1776 1776 a, g dbSNP:569762069
1779 1779 c, t dbSNP:185031539
1849 1849 g, t dbSNP:113189897
1854 1854 c, t dbSNP:555030859
1857 1857 c, t dbSNP:567575430
1863 1863 g, t dbSNP:534642379
1865 1865 c, g dbSNP:370627204
1877 1877 c, t dbSNP:577455713
1878 1878 g, t dbSNP:749317017
1882 1882 a, g dbSNP:190272500
1889 1889 c, t dbSNP:9920576
1899 1899 a, c dbSNP:11632190
1901 1901 c, t dbSNP:15880
1903 1903 c, t dbSNP:747847709
1906 1906 a, g dbSNP:771881952
1937 1937 c, g dbSNP:372644159
1953 1953 -, tg dbSNP:762843585
1962 1962 a, c dbSNP:58853216
1964 1964 a, g dbSNP:561904883
1965 1965 a, t dbSNP:573955623
2002 2002 c, t dbSNP:574075039
2003 2003 a, g dbSNP:192526701
2029 2029 c, t dbSNP:760378631

Target ORF information:

RefSeq Version XM_005254645
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005254645

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATCAACTC
TACCAGCAAC TTGAGCAAAA TCGCCGCCTC ACTAATGAAC TAAAGCTGGC CCTGAATGAG
GATTAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu48546D
Sequence Information ORF Nucleotide Sequence (Length: 828bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TCCGACAGCT GGAAGAACAA
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGGTAAGA TCTTAAGTAG TGTTTTTAGT TTAATCCTTA TGGTTGAATA CCAACCTGGC
AAAACAATTT TCCAATTCAA GGGCATCCAC ATTGATACGC TCCTTTGCAC TTGCACATTC
TTCCTGTGTG TCCTCTGGGG TTTTTCTCTG TGGCTCTTGA ACTCATGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005254703.1
CDS126..953
Misc Feature(1)159..803(+)
Misc Feature(2)159..476(+)
Translation
Position Chain Variation Link
23 23 c, t dbSNP:754685532
27 27 c, t dbSNP:375924161
28 28 c, t dbSNP:767366662
34 34 -, t dbSNP:146654937
49 49 c, g dbSNP:538759113
54 54 a, g dbSNP:540091293
56 56 c, t dbSNP:111470259
76 76 -, c dbSNP:771295189
84 84 c, t dbSNP:748866546
91 91 a, c dbSNP:756805858
109 109 a, g dbSNP:778363083
132 132 a, g dbSNP:397516490
149 149 a, g dbSNP:768857765
156 156 c, t dbSNP:777033105
159 159 a, g, t dbSNP:397516489
166 166 c, t dbSNP:200927546
167 167 c, g dbSNP:730881129
172 172 g, t dbSNP:199476308
182 182 c, g dbSNP:770341251
183 183 c, g, t dbSNP:543980919
188 188 a, g dbSNP:771069931
191 191 c, g dbSNP:563678042
192 192 a, g dbSNP:142657113
200 200 a, g dbSNP:549116295
215 215 c, t dbSNP:377063875
233 233 c, g dbSNP:373786931
236 236 c, t dbSNP:767388543
265 265 a, c dbSNP:730881130
266 266 c, t dbSNP:200257214
267 267 a, g dbSNP:754664923
269 269 c, g, t dbSNP:369617788
270 270 a, g dbSNP:730881156
274 274 c, g dbSNP:757577112
281 281 a, g dbSNP:779411131
292 292 c, t dbSNP:199476310
293 293 c, g dbSNP:730881157
295 295 a, g dbSNP:746394586
299 299 a, g dbSNP:758503054
301 301 c, t dbSNP:104894504
305 305 a, g dbSNP:192883939
309 309 c, g dbSNP:730881131
310 310 a, g dbSNP:730881158
311 311 a, g dbSNP:747206706
318 318 c, g, t dbSNP:769069020
319 319 a, g dbSNP:747907799
322 322 a, c dbSNP:397516367
323 323 c, g dbSNP:769505504
325 325 a, g dbSNP:112125078
331 331 a, g dbSNP:773149185
338 338 a, c dbSNP:397516368
340 340 c, t dbSNP:762922595
350 350 a, g dbSNP:765843551
354 354 c, g dbSNP:397516369
358 358 a, g dbSNP:397516370
360 360 a, g dbSNP:727504313
371 371 g, t dbSNP:773916751
372 372 a, g dbSNP:759173967
398 398 a, g dbSNP:201211957
402 402 -, gt dbSNP:779940940
404 404 a, c, t dbSNP:769605281
406 406 c, t dbSNP:727503517
420 420 a, c dbSNP:11558749
427 427 a, g, t dbSNP:730881134
430 430 a, g dbSNP:786204410
433 433 a, t dbSNP:727504389
435 435 a, c dbSNP:730881135
440 440 c, g dbSNP:397516371
445 445 g, t dbSNP:730881136
461 461 c, g dbSNP:376518788
464 464 a, g dbSNP:774283931
470 470 a, c dbSNP:1071646
474 474 c, g dbSNP:397516372
475 475 a, g dbSNP:727505043
476 476 a, c dbSNP:771515165
491 491 c, t dbSNP:143922069
492 492 a, g dbSNP:397516373
496 496 a, g dbSNP:199476311
503 503 c, t dbSNP:11558747
524 524 a, g dbSNP:139663836
528 528 a, g dbSNP:774903903
530 530 c, g dbSNP:730881137
531 531 a, c dbSNP:760374266
532 532 c, t dbSNP:199476312
533 533 g, t dbSNP:730881138
539 539 c, t dbSNP:200173919
540 540 a, g dbSNP:104894503
549 549 c, t dbSNP:367606352
550 550 a, g dbSNP:397516375
556 556 a, g, t dbSNP:104894502
565 565 c, t dbSNP:397516376
566 566 c, g, t dbSNP:199476313
568 568 -, a dbSNP:730881153
571 571 g, t dbSNP:199476314
572 572 c, t dbSNP:752088217
576 576 c, g dbSNP:727504264
577 577 a, t dbSNP:786204411
588 588 a, c, t dbSNP:201813515
589 589 a, g dbSNP:141757680
605 605 a, g dbSNP:765013065
611 611 a, t dbSNP:200794206
625 625 c, g dbSNP:766058944
627 627 c, t dbSNP:779791290
634 634 c, t dbSNP:747001359
635 635 a, g dbSNP:144700226
661 661 c, t dbSNP:199476316
662 662 a, g dbSNP:773403386
672 672 a, g dbSNP:727503518
676 676 a, g dbSNP:763069444
680 680 c, t dbSNP:774859181
686 686 a, g dbSNP:573495443
690 690 a, g dbSNP:193922410
693 693 a, c dbSNP:397516383
694 694 a, g dbSNP:730881144
701 701 c, t dbSNP:397516384
704 704 c, t dbSNP:759362606
705 705 a, g dbSNP:199476317
709 709 a, g dbSNP:397516385
729 729 c, t dbSNP:397516386
730 730 a, g dbSNP:777716347
732 732 a, g dbSNP:199476318
742 742 c, t dbSNP:397516387
743 743 a, g dbSNP:200484871
751 751 c, t dbSNP:397516388
752 752 a, c, g dbSNP:771961466
757 757 a, c dbSNP:760516389
759 759 a, g dbSNP:199476319
760 760 a, c dbSNP:730881160
763 763 g, t dbSNP:786204412
770 770 a, g dbSNP:764036919
779 779 g, t dbSNP:727504354
785 785 a, g dbSNP:139650306
790 790 a, g dbSNP:113368770
801 801 a, g dbSNP:761349063
804 804 a, g dbSNP:764753829
814 814 c, g dbSNP:750059091
825 825 a, g dbSNP:375508214
829 829 a, t dbSNP:376976043
833 833 a, t dbSNP:753045789
834 834 c, t dbSNP:756435612
840 840 -, c dbSNP:746894840
840 840 c, t dbSNP:538085950
852 852 a, g dbSNP:372214593
860 860 a, c, g dbSNP:569383169
866 866 a, g dbSNP:746085373
868 868 a, g dbSNP:772481168
870 870 a, t dbSNP:775869894
883 883 cgctcc, tt dbSNP:397515630
883 883 -, cgc dbSNP:57598555
883 883 a, c, t dbSNP:746753372
883 883 c, tt dbSNP:193922411
885 885 c, t dbSNP:776585935
887 887 -, c dbSNP:58882433
888 888 c, t dbSNP:56054026
893 893 c, g dbSNP:764698939
899 899 c, t dbSNP:772764185
905 905 c, t dbSNP:762459188
909 909 c, t dbSNP:765946293
911 911 a, g dbSNP:751198820
921 921 -, g dbSNP:35278823
925 925 a, g dbSNP:777519708
931 931 c, t dbSNP:555024045
933 933 c, t dbSNP:573182762
934 934 g, t dbSNP:754339678
937 937 c, g dbSNP:746916388
944 944 g, t dbSNP:186484541
952 952 c, g dbSNP:190305952
960 960 a, g dbSNP:779124171
965 965 a, c dbSNP:773865422
967 967 a, g dbSNP:144670024
972 972 c, t dbSNP:182481458
973 973 -, g dbSNP:772606005
990 990 a, c dbSNP:200925955
994 994 a, g dbSNP:780385364
997 997 a, g dbSNP:747220478
1020 1020 g, t dbSNP:544415066
1033 1033 a, g dbSNP:770902938
1035 1035 -, c dbSNP:750192930
1037 1037 a, c, g dbSNP:12442826
1074 1074 a, g dbSNP:771712597
1115 1115 a, g dbSNP:148203848
1147 1147 a, g dbSNP:541554334
1172 1172 a, g dbSNP:560178311
1192 1192 a, c dbSNP:527337816
1214 1214 c, g dbSNP:545781648
1220 1220 a, t dbSNP:11071723
1224 1224 a, t dbSNP:531595370
1226 1226 a, g dbSNP:775079270
1238 1238 -, g dbSNP:35851171
1260 1260 c, g dbSNP:184405423
1284 1284 c, t dbSNP:12148828
1318 1318 a, g dbSNP:529076885
1322 1322 c, t dbSNP:548425328
1328 1328 g, t dbSNP:115521315
1376 1376 c, t dbSNP:533982288
1379 1379 a, g dbSNP:558861959
1400 1400 a, g dbSNP:370070818
1416 1416 c, t dbSNP:55982639
1482 1482 a, g dbSNP:761146266
1487 1487 a, g dbSNP:764392088

Target ORF information:

RefSeq Version XM_005254646
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005254646

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TCCGACAGCT GGAAGAACAA
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGGTAAGA TCTTAAGTAG TGTTTTTAGT TTAATCCTTA TGGTTGAATA CCAACCTGGC
AAAACAATTT TCCAATTCAA GGGCATCCAC ATTGATACGC TCCTTTGCAC TTGCACATTC
TTCCTGTGTG TCCTCTGGGG TTTTTCTCTG TGGCTCTTGA ACTCATGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu48547D
Sequence Information ORF Nucleotide Sequence (Length: 756bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530406405. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACTG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATAAGT TTCTTTGCTT CACTTCTCCC AAGACTCCCT CGTCGAGCTG GATGTCCCAC
CTCTCTGAGC TCTGCATTTG TCTATTCTCC AGCTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005254704.1
CDS126..881
Misc Feature(1)159..794(+)
Misc Feature(2)159..476(+)
Translation
Position Chain Variation Link
23 23 c, t dbSNP:754685532
27 27 c, t dbSNP:375924161
28 28 c, t dbSNP:767366662
34 34 -, t dbSNP:146654937
49 49 c, g dbSNP:538759113
54 54 a, g dbSNP:540091293
56 56 c, t dbSNP:111470259
76 76 -, c dbSNP:771295189
84 84 c, t dbSNP:748866546
91 91 a, c dbSNP:756805858
109 109 a, g dbSNP:778363083
132 132 a, g dbSNP:397516490
149 149 a, g dbSNP:768857765
156 156 c, t dbSNP:777033105
159 159 a, g, t dbSNP:397516489
166 166 c, t dbSNP:200927546
167 167 c, g dbSNP:730881129
172 172 g, t dbSNP:199476308
182 182 c, g dbSNP:770341251
183 183 c, g, t dbSNP:543980919
188 188 a, g dbSNP:771069931
191 191 c, g dbSNP:563678042
192 192 a, g dbSNP:142657113
200 200 a, g dbSNP:549116295
215 215 c, t dbSNP:377063875
233 233 c, g dbSNP:373786931
236 236 c, t dbSNP:767388543
265 265 a, c dbSNP:730881130
266 266 c, t dbSNP:200257214
267 267 a, g dbSNP:754664923
269 269 c, g, t dbSNP:369617788
270 270 a, g dbSNP:730881156
274 274 c, g dbSNP:757577112
281 281 a, g dbSNP:779411131
292 292 c, t dbSNP:199476310
293 293 c, g dbSNP:730881157
295 295 a, g dbSNP:746394586
299 299 a, g dbSNP:758503054
301 301 c, t dbSNP:104894504
305 305 a, g dbSNP:192883939
309 309 c, g dbSNP:730881131
310 310 a, g dbSNP:730881158
311 311 a, g dbSNP:747206706
318 318 c, g, t dbSNP:769069020
319 319 a, g dbSNP:747907799
322 322 a, c dbSNP:397516367
323 323 c, g dbSNP:769505504
325 325 a, g dbSNP:112125078
331 331 a, g dbSNP:773149185
338 338 a, c dbSNP:397516368
340 340 c, t dbSNP:762922595
350 350 a, g dbSNP:765843551
354 354 c, g dbSNP:397516369
358 358 a, g dbSNP:397516370
360 360 a, g dbSNP:727504313
371 371 g, t dbSNP:773916751
372 372 a, g dbSNP:759173967
398 398 a, g dbSNP:201211957
402 402 -, gt dbSNP:779940940
404 404 a, c, t dbSNP:769605281
406 406 c, t dbSNP:727503517
420 420 a, c dbSNP:11558749
427 427 a, g, t dbSNP:730881134
430 430 a, g dbSNP:786204410
433 433 a, t dbSNP:727504389
435 435 a, c dbSNP:730881135
440 440 c, g dbSNP:397516371
445 445 g, t dbSNP:730881136
461 461 c, g dbSNP:376518788
464 464 a, g dbSNP:774283931
470 470 a, c dbSNP:1071646
474 474 c, g dbSNP:397516372
475 475 a, g dbSNP:727505043
476 476 a, c dbSNP:771515165
491 491 c, t dbSNP:143922069
492 492 a, g dbSNP:397516373
496 496 a, g dbSNP:199476311
503 503 c, t dbSNP:11558747
524 524 a, g dbSNP:139663836
528 528 a, g dbSNP:774903903
530 530 c, g dbSNP:730881137
531 531 a, c dbSNP:760374266
532 532 c, t dbSNP:199476312
533 533 g, t dbSNP:730881138
539 539 c, t dbSNP:200173919
540 540 a, g dbSNP:104894503
549 549 c, t dbSNP:367606352
550 550 a, g dbSNP:397516375
556 556 a, g, t dbSNP:104894502
565 565 c, t dbSNP:397516376
566 566 c, g, t dbSNP:199476313
568 568 -, a dbSNP:730881153
571 571 g, t dbSNP:199476314
572 572 c, t dbSNP:752088217
576 576 c, g dbSNP:727504264
577 577 a, t dbSNP:786204411
585 585 c, t dbSNP:730881139
589 589 cc, tt dbSNP:730881154
589 589 c, t dbSNP:772303740
590 590 c, t dbSNP:780507307
591 591 a, g dbSNP:199476315
596 596 g, t dbSNP:1131002
602 602 a, g dbSNP:3218717
603 603 c, g dbSNP:1131003
609 609 a, g dbSNP:730881140
619 619 c, t dbSNP:730881141
620 620 a, c, g dbSNP:397516485
626 626 c, g dbSNP:397516486
637 637 -, t dbSNP:587777905
642 642 a, g dbSNP:730881159
644 644 g, t dbSNP:769210513
646 646 a, c dbSNP:777139450
647 647 a, g dbSNP:761816813
649 649 c, g dbSNP:397516487
651 651 -, ga dbSNP:199990219
652 652 a, t dbSNP:769951937
653 653 g, t dbSNP:17416757
661 661 c, t dbSNP:199476316
662 662 a, g dbSNP:773403386
672 672 a, g dbSNP:727503518
676 676 a, g dbSNP:763069444
680 680 c, t dbSNP:774859181
686 686 a, g dbSNP:573495443
690 690 a, g dbSNP:193922410
693 693 a, c dbSNP:397516383
694 694 a, g dbSNP:730881144
701 701 c, t dbSNP:397516384
704 704 c, t dbSNP:759362606
705 705 a, g dbSNP:199476317
709 709 a, g dbSNP:397516385
729 729 c, t dbSNP:397516386
730 730 a, g dbSNP:777716347
732 732 a, g dbSNP:199476318
742 742 c, t dbSNP:397516387
743 743 a, g dbSNP:200484871
751 751 c, t dbSNP:397516388
752 752 a, c, g dbSNP:771961466
757 757 a, c dbSNP:760516389
759 759 a, g dbSNP:199476319
760 760 a, c dbSNP:730881160
763 763 g, t dbSNP:786204412
770 770 a, g dbSNP:764036919
779 779 g, t dbSNP:727504354
785 785 a, g dbSNP:139650306
790 790 a, t dbSNP:759481997
795 795 -, tttc dbSNP:772897124
798 798 -, cttt dbSNP:786204415
799 799 g, t dbSNP:767681625
804 804 g, t dbSNP:752883071
811 811 c, t dbSNP:756393228
814 814 a, c dbSNP:146861835
823 823 c, t dbSNP:753572625
824 824 c, g, t dbSNP:757080224
826 826 c, t dbSNP:745841842
827 827 a, g dbSNP:778395303
829 829 c, t dbSNP:538518251
830 830 a, c, g dbSNP:201563826
841 841 c, g, t dbSNP:575130839
846 846 c, g dbSNP:780992464
856 856 c, t dbSNP:749572083
857 857 c, t dbSNP:771345862
861 861 a, g dbSNP:374459540
868 868 a, t dbSNP:760126994
869 869 a, g dbSNP:140658011
874 874 c, t dbSNP:775604378
880 880 c, g dbSNP:760757735
885 885 c, t dbSNP:764235066
887 887 a, g dbSNP:754091745
892 892 c, g, t dbSNP:150024529
898 898 c, t dbSNP:750300077
921 921 c, t dbSNP:540306699
932 932 c, t dbSNP:145513507
938 938 a, g dbSNP:148842623
941 941 g, t dbSNP:7668
948 948 a, g dbSNP:533687214
955 955 c, t dbSNP:775923793
968 968 a, g dbSNP:545752658
977 977 c, t dbSNP:764354563
1007 1007 c, t dbSNP:774595807
1041 1041 c, t dbSNP:563965885
1052 1052 c, t dbSNP:531178841

Target ORF information:

RefSeq Version XM_005254647
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005254647

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACTG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATAAGT TTCTTTGCTT CACTTCTCCC AAGACTCCCT CGTCGAGCTG GATGTCCCAC
CTCTCTGAGC TCTGCATTTG TCTATTCTCC AGCTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu48548D
Sequence Information ORF Nucleotide Sequence (Length: 747bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACTG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGACGAGC TGTACGCTCA GAAACTGAAG TACAAAGCCA TCAGCGAGGA GCTGGACCAC
GCTCTCAACG ATATGACTTC CATGTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005254705.1
CDS126..872
Misc Feature(1)159..869(+)
Misc Feature(2)159..476(+)
Translation
Position Chain Variation Link
23 23 c, t dbSNP:754685532
27 27 c, t dbSNP:375924161
28 28 c, t dbSNP:767366662
34 34 -, t dbSNP:146654937
49 49 c, g dbSNP:538759113
54 54 a, g dbSNP:540091293
56 56 c, t dbSNP:111470259
76 76 -, c dbSNP:771295189
84 84 c, t dbSNP:748866546
91 91 a, c dbSNP:756805858
109 109 a, g dbSNP:778363083
132 132 a, g dbSNP:397516490
149 149 a, g dbSNP:768857765
156 156 c, t dbSNP:777033105
159 159 a, g, t dbSNP:397516489
166 166 c, t dbSNP:200927546
167 167 c, g dbSNP:730881129
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