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TPM1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol TPM1
Entrez Gene ID 7168
Full Name tropomyosin 1 (alpha)
Synonyms C15orf13, CMD1Y, CMH3, HTM-alpha, LVNC9, TMSA
General protein information
Preferred Names
tropomyosin alpha-1 chain
Names
tropomyosin alpha-1 chain
alpha-tropomyosin
sarcomeric tropomyosin kappa
cardiomyopathy, hypertrophic 3
tropomyosin 1 (alpha) isoform 1
tropomyosin 1 (alpha) isoform 2
tropomyosin 1 (alpha) isoform 3
tropomyosin 1 (alpha) isoform 4
tropomyosin 1 (alpha) isoform 5
tropomyosin 1 (alpha) isoform 6
tropomyosin 1 (alpha) isoform 7
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q22.1

Summary This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

191010

Disorder Html: Cardiomyopathy, familial hypertrophic, 3, 115196 (3); Cardiomyopathy,

mRNA and Protein(s)

mRNA Protein Name
XM_005254638 XP_005254695 tropomyosin alpha-1 chain isoform X1
XM_005254640 XP_005254697 tropomyosin alpha-1 chain isoform X2
XM_005254639 XP_005254696 tropomyosin alpha-1 chain isoform X3
XM_005254641 XP_005254698 tropomyosin alpha-1 chain isoform X4
XM_006720667 XP_006720730 tropomyosin alpha-1 chain isoform X5
XM_005254645 XP_005254702 tropomyosin alpha-1 chain isoform X6
XM_005254646 XP_005254703 tropomyosin alpha-1 chain isoform X7
XM_005254647 XP_005254704 tropomyosin alpha-1 chain isoform X8
XM_005254648 XP_005254705 tropomyosin alpha-1 chain isoform X9
XM_005254650 XP_005254707 tropomyosin alpha-1 chain isoform X10
XM_005254651 XP_005254708 tropomyosin alpha-1 chain isoform X11
XM_005254652 XP_005254709 tropomyosin alpha-1 chain isoform X12
XM_005254653 XP_005254710 tropomyosin alpha-1 chain isoform X13
XM_006720669 XP_006720732 tropomyosin alpha-1 chain isoform X14
XM_005254637 XP_005254694 tropomyosin alpha-1 chain isoform X15
NM_001018008 NP_001018008 tropomyosin alpha-1 chain isoform Tpm1.12br
NM_001018006 NP_001018006 tropomyosin alpha-1 chain isoform Tpm1.7cy
NM_001018004 NP_001018004 tropomyosin alpha-1 chain isoform Tpm1.6cy
NM_001301289 NP_001288218 tropomyosin alpha-1 chain isoform Tpm1.8cy
NM_001301244 NP_001288173 tropomyosin alpha-1 chain isoform Tpm1.2st
NM_001018005 NP_001018005 tropomyosin alpha-1 chain isoform Tpm1.1st
NM_000366 NP_000357 tropomyosin alpha-1 chain isoform Tpm1.5cy
NM_001018020 NP_001018020 tropomyosin alpha-1 chain isoform Tpm1.3sm
NM_001018007 NP_001018007 tropomyosin alpha-1 chain isoform Tpm1.4sm



Homo sapiens (human) TPM1 NP_001018004.1
Pan troglodytes (chimpanzee) TPM1 XP_001173556.2
Macaca mulatta (Rhesus monkey) TPM1 XP_001104296.1
Bos taurus (cattle) TPM1 NP_001013608.1
Gallus gallus (chicken) TPM1 NP_990732.1
Danio rerio (zebrafish) tpm2 NP_001002119.1
Drosophila melanogaster (fruit fly) Tm1 NP_732001.3
Caenorhabditis elegans lev-11 NP_001021695.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TPM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu48538 XM_005254638 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48539 XM_005254640 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48540 XM_005254639 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48541 XM_005254641 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48544 XM_006720667 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48545 XM_005254645 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48546 XM_005254646 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48547 XM_005254647 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48548 XM_005254648 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48550 XM_005254650 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48551 XM_005254651 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48552 XM_005254652 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48553 XM_005254653 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48554 XM_006720669 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu48555 XM_005254637 PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu16163
NM_001018008 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu21700
NM_001018006 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
OHu21508 NM_001018004 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48549 NM_001301289 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu48542 NM_001301244 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu19397
NM_001018005 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
OHu16327
NM_000366 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu20883 NM_001018020 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu16265 NM_001018007 Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu48538
Clone ID Related Accession (Same CDS sequence) XM_005254638
Accession Version XM_005254638.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530406387. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254695.1
CDS173..1036
Misc Feature(1)191..631(+)
Misc Feature(2)314..949(+)
Translation

Target ORF information:

RefSeq Version XM_005254638
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254638

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48539
Clone ID Related Accession (Same CDS sequence) XM_005254640
Accession Version XM_005254640.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530406391. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254697.1
CDS147..1010
Misc Feature(1)165..605(+)
Misc Feature(2)288..923(+)
Translation

Target ORF information:

RefSeq Version XM_005254640
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254640

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48540
Clone ID Related Accession (Same CDS sequence) XM_005254639
Accession Version XM_005254639.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530406389. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254696.1
CDS173..1036
Misc Feature(1)191..631(+)
Misc Feature(2)314..949(+)
Translation

Target ORF information:

RefSeq Version XM_005254639
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254639

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48541
Clone ID Related Accession (Same CDS sequence) XM_005254641
Accession Version XM_005254641.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530406393. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254698.1
CDS147..1010
Misc Feature(1)165..605(+)
Misc Feature(2)288..923(+)
Translation

Target ORF information:

RefSeq Version XM_005254641
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254641

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTTCTCCCAA GACTCCCTCG TCGAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATTTGTC TATTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48544
Clone ID Related Accession (Same CDS sequence) XM_006720667
Accession Version XM_006720667.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578827354. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TACGCTCAGA AACTGAAGTA CAAAGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006720730.1
CDS187..1041
Misc Feature(1)205..645(+)
Misc Feature(2)328..1038(+)
Translation

Target ORF information:

RefSeq Version XM_006720667
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006720667

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TACGCTCAGA AACTGAAGTA CAAAGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48545
Clone ID Related Accession (Same CDS sequence) XM_005254645
Accession Version XM_005254645.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 846bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATCAACTC
TACCAGCAAC TTGAGCAAAA TCGCCGCCTC ACTAATGAAC TAAAGCTGGC CCTGAATGAG
GATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254702.1
CDS173..1018
Misc Feature(1)191..631(+)
Misc Feature(2)314..1012(+)
Translation

Target ORF information:

RefSeq Version XM_005254645
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254645

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGATCAACTC
TACCAGCAAC TTGAGCAAAA TCGCCGCCTC ACTAATGAAC TAAAGCTGGC CCTGAATGAG
GATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48546
Clone ID Related Accession (Same CDS sequence) XM_005254646
Accession Version XM_005254646.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 828bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TCCGACAGCT GGAAGAACAA
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGGTAAGA TCTTAAGTAG TGTTTTTAGT TTAATCCTTA TGGTTGAATA CCAACCTGGC
AAAACAATTT TCCAATTCAA GGGCATCCAC ATTGATACGC TCCTTTGCAC TTGCACATTC
TTCCTGTGTG TCCTCTGGGG TTTTTCTCTG TGGCTCTTGA ACTCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254703.1
CDS126..953
Misc Feature(1)159..803(+)
Misc Feature(2)159..476(+)
Translation

Target ORF information:

RefSeq Version XM_005254646
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254646

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TCCGACAGCT GGAAGAACAA
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGGTAAGA TCTTAAGTAG TGTTTTTAGT TTAATCCTTA TGGTTGAATA CCAACCTGGC
AAAACAATTT TCCAATTCAA GGGCATCCAC ATTGATACGC TCCTTTGCAC TTGCACATTC
TTCCTGTGTG TCCTCTGGGG TTTTTCTCTG TGGCTCTTGA ACTCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48547
Clone ID Related Accession (Same CDS sequence) XM_005254647
Accession Version XM_005254647.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 756bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530406405. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACTG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATAAGT TTCTTTGCTT CACTTCTCCC AAGACTCCCT CGTCGAGCTG GATGTCCCAC
CTCTCTGAGC TCTGCATTTG TCTATTCTCC AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254704.1
CDS126..881
Misc Feature(1)159..794(+)
Misc Feature(2)159..476(+)
Translation

Target ORF information:

RefSeq Version XM_005254647
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254647

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACTG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATAAGT TTCTTTGCTT CACTTCTCCC AAGACTCCCT CGTCGAGCTG GATGTCCCAC
CTCTCTGAGC TCTGCATTTG TCTATTCTCC AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48548
Clone ID Related Accession (Same CDS sequence) XM_005254648
Accession Version XM_005254648.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 747bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACTG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGACGAGC TGTACGCTCA GAAACTGAAG TACAAAGCCA TCAGCGAGGA GCTGGACCAC
GCTCTCAACG ATATGACTTC CATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254705.1
CDS126..872
Misc Feature(1)159..869(+)
Misc Feature(2)159..476(+)
Translation

Target ORF information:

RefSeq Version XM_005254648
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254648

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACTG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGACGAGC TGTACGCTCA GAAACTGAAG TACAAAGCCA TCAGCGAGGA GCTGGACCAC
GCTCTCAACG ATATGACTTC CATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48550
Clone ID Related Accession (Same CDS sequence) XM_005254650
Accession Version XM_005254650.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 747bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530406411. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TCCGACAGCT GGAAGAACAA
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGACGAGC TGTACGCTCA GAAACTGAAG TACAAAGCCA TCAGCGAGGA GCTGGACCAC
GCTCTCAACG ATATGACTTC CATATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254707.1
CDS126..872
Misc Feature(1)159..869(+)
Misc Feature(2)159..476(+)
Translation

Target ORF information:

RefSeq Version XM_005254650
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254650

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TCCGACAGCT GGAAGAACAA
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGACGAGC TGTACGCTCA GAAACTGAAG TACAAAGCCA TCAGCGAGGA GCTGGACCAC
GCTCTCAACG ATATGACTTC CATATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48551
Clone ID Related Accession (Same CDS sequence) XM_005254651
Accession Version XM_005254651.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 747bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X11
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TCCGACAGCT GGAAGAACAA
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGACGAGC TGTACGCTCA GAAACTGAAG TACAAAGCCA TCAGCGAGGA GCTGGACCAC
GCTCTCAACG ATATGACTTC CATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254708.1
CDS126..872
Misc Feature(1)159..869(+)
Misc Feature(2)159..476(+)
Translation

Target ORF information:

RefSeq Version XM_005254651
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254651

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TCCGACAGCT GGAAGAACAA
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGACGAGC TGTACGCTCA GAAACTGAAG TACAAAGCCA TCAGCGAGGA GCTGGACCAC
GCTCTCAACG ATATGACTTC CATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48552
Clone ID Related Accession (Same CDS sequence) XM_005254652
Accession Version XM_005254652.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 747bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TCCGACAGCT GGAAGAACAA
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGAGAAAG TGGCTCATGC CAAAGAAGAA AACCTTAGTA TGCATCAGAT GCTGGATCAG
ACTTTACTGG AGTTAAACAA CATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254709.1
CDS126..872
Misc Feature(1)159..869(+)
Misc Feature(2)159..476(+)
Translation

Target ORF information:

RefSeq Version XM_005254652
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254652

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TCCGACAGCT GGAAGAACAA
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGAGAAAG TGGCTCATGC CAAAGAAGAA AACCTTAGTA TGCATCAGAT GCTGGATCAG
ACTTTACTGG AGTTAAACAA CATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48553
Clone ID Related Accession (Same CDS sequence) XM_005254653
Accession Version XM_005254653.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 738bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X13
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TCCGACAGCT GGAAGAACAA
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATCAAC TCTACCAGCA ACTTGAGCAA AATCGCCGCC TCACTAATGA ACTAAAGCTG
GCCCTGAATG AGGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254710.1
CDS126..863
Misc Feature(1)159..857(+)
Misc Feature(2)159..476(+)
Translation

Target ORF information:

RefSeq Version XM_005254653
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254653

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TCCGACAGCT GGAAGAACAA
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATCAAC TCTACCAGCA ACTTGAGCAA AATCGCCGCC TCACTAATGA ACTAAAGCTG
GCCCTGAATG AGGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48554
Clone ID Related Accession (Same CDS sequence) XM_006720669
Accession Version XM_006720669.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 486bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X14
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578827358. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA GATGAAGAAA AAATGGAAAT TCAGGAGATC 
CAACTGAAAG AGGCCAAGCA CATTGCTGAA GATGCCGACC GCAAATATGA AGAGGTGGCC
CGTAAGCTGG TCATCATTGA GAGCGACCTG GAACGTGCAG AGGAGCGGGC TGAGCTCTCA
GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA TTGAAAACTG TGACGAACAA CTTGAAGTCA
CTGGAGGCTC AGGCTGAGAA GTACTCGCAG AAGGAAGACA GATATGAGGA AGAGATCAAG
GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA
ACTAAATTGG AGAAAAGCAT TGATGACTTA GAAGATAAGT TTCTTTGCTT CACTTCTCCC
AAGACTCCCT CGTCGAGCTG GATGTCCCAC CTCTCTGAGC TCTGCATTTG TCTATTCTCC
AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006720732.1
CDS808..1293
Misc Feature(1)808..1206(+)
Misc Feature(2)844..1209(+)
Translation

Target ORF information:

RefSeq Version XM_006720669
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006720669

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA GATGAAGAAA AAATGGAAAT TCAGGAGATC 
CAACTGAAAG AGGCCAAGCA CATTGCTGAA GATGCCGACC GCAAATATGA AGAGGTGGCC
CGTAAGCTGG TCATCATTGA GAGCGACCTG GAACGTGCAG AGGAGCGGGC TGAGCTCTCA
GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA TTGAAAACTG TGACGAACAA CTTGAAGTCA
CTGGAGGCTC AGGCTGAGAA GTACTCGCAG AAGGAAGACA GATATGAGGA AGAGATCAAG
GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA
ACTAAATTGG AGAAAAGCAT TGATGACTTA GAAGATAAGT TTCTTTGCTT CACTTCTCCC
AAGACTCCCT CGTCGAGCTG GATGTCCCAC CTCTCTGAGC TCTGCATTTG TCTATTCTCC
AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48555
Clone ID Related Accession (Same CDS sequence) XM_005254637
Accession Version XM_005254637.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform X15
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
CTGGAAGATG AGCTGGTGTC ACTGCAAAAG AAACTCAAGG GCACCGAAGA TGAACTGGAC
AAATACTCTG AGGCTCTCAA AGATGCCCAG GAGAAGCTGG AGCTGGCAGA GAAAAAGGCC
ACCGATGCTG AAGCCGACGT AGCTTCTCTG AACAGACGCA TCCAGCTGGT TGAGGAAGAG
TTGGATCGTG CCCAGGAGCG TCTGGCAACA GCTTTGCAGA AGCTGGAGGA AGCTGAGAAG
GCAGCAGATG AGAGTGAGAG AGGCATGAAA GTCATTGAGA GTCGAGCCCA AAAAGATGAA
GAAAAAATGG AAATTCAGGA GATCCAACTG AAAGAGGCCA AGCACATTGC TGAAGATGCC
GACCGCAAAT ATGAAGAGGT GGCCCGTAAG CTGGTCATCA TTGAGAGCGA CCTGGAACGT
GCAGAGGAGC GGGCTGAGCT CTCAGAAGGC AAATGTGCCG AGCTTGAAGA AGAATTGAAA
ACTGTGACGA ACAACTTGAA GTCACTGGAG GCTCAGGCTG AGAAGTACTC GCAGAAGGAA
GACAGATATG AGGAAGAGAT CAAGGTCCTT TCCGACAAGC TGAAGGAGGC TGAGACTCGG
GCTGAGTTTG CGGAGAGGTC AGTAACTAAA TTGGAGAAAA GCATTGATGA CTTAGAAGAG
AAAGTGGCTC ATGCCAAAGA AGAAAACCTT AGTATGCATC AGATGCTGGA TCAGACTTTA
CTGGAGTTAA ACAACATGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254694.1
CDS147..1127
Misc Feature(1)165..731(+)
Misc Feature(2)414..1124(+)
Translation

Target ORF information:

RefSeq Version XM_005254637
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254637

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
CTGGAAGATG AGCTGGTGTC ACTGCAAAAG AAACTCAAGG GCACCGAAGA TGAACTGGAC
AAATACTCTG AGGCTCTCAA AGATGCCCAG GAGAAGCTGG AGCTGGCAGA GAAAAAGGCC
ACCGATGCTG AAGCCGACGT AGCTTCTCTG AACAGACGCA TCCAGCTGGT TGAGGAAGAG
TTGGATCGTG CCCAGGAGCG TCTGGCAACA GCTTTGCAGA AGCTGGAGGA AGCTGAGAAG
GCAGCAGATG AGAGTGAGAG AGGCATGAAA GTCATTGAGA GTCGAGCCCA AAAAGATGAA
GAAAAAATGG AAATTCAGGA GATCCAACTG AAAGAGGCCA AGCACATTGC TGAAGATGCC
GACCGCAAAT ATGAAGAGGT GGCCCGTAAG CTGGTCATCA TTGAGAGCGA CCTGGAACGT
GCAGAGGAGC GGGCTGAGCT CTCAGAAGGC AAATGTGCCG AGCTTGAAGA AGAATTGAAA
ACTGTGACGA ACAACTTGAA GTCACTGGAG GCTCAGGCTG AGAAGTACTC GCAGAAGGAA
GACAGATATG AGGAAGAGAT CAAGGTCCTT TCCGACAAGC TGAAGGAGGC TGAGACTCGG
GCTGAGTTTG CGGAGAGGTC AGTAACTAAA TTGGAGAAAA GCATTGATGA CTTAGAAGAG
AAAGTGGCTC ATGCCAAAGA AGAAAACCTT AGTATGCATC AGATGCTGGA TCAGACTTTA
CTGGAGTTAA ACAACATGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16163
Clone ID Related Accession (Same CDS sequence) NM_001018008
Accession Version NM_001018008.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 738bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform Tpm1.12br
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079328.11, BC053545.1 and BC050473.1. Transcript Variant: This variant (Tpm1.12, also known as variant 6) contains alternate in-frame exons in the 5' and 3' coding region, compared to variant Tpm1.1. It encodes isoform Tpm1.12br, also known as the TMBr-3 brain isoform, which has distinct N- and C-termini and is shorter than isoform Tpm1.1st. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053545.1, AB209041.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACTG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATCAAC TCTACCAGCA ACTTGAGCAA AATCGCCGCC TCACTAATGA ACTAAAGCTG
GCCCTGAATG AGGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001018008.1
CDS140..877
Misc Feature(1)140..142(+)
Misc Feature(2)173..871(+)
Misc Feature(3)173..490(+)
Exon (1)1..271
Gene:TPM1
Gene Synonym:
Exon (2)272..405
Gene:TPM1
Gene Synonym:
Exon (3)406..523
Gene:TPM1
Gene Synonym:
Exon (4)524..594
Gene:TPM1
Gene Synonym:
Exon (5)595..670
Gene:TPM1
Gene Synonym:
Exon (6)671..733
Gene:TPM1
Gene Synonym:
Exon (7)734..803
Gene:TPM1
Gene Synonym:
Exon (8)804..1566
Gene:TPM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001018008
Organism Homo sapiens (human)
Definition Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001018008

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACTG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATCAAC TCTACCAGCA ACTTGAGCAA AATCGCCGCC TCACTAATGA ACTAAAGCTG
GCCCTGAATG AGGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21700
Clone ID Related Accession (Same CDS sequence) NM_001018006
Accession Version NM_001018006.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform Tpm1.7cy
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG107949.1, AC079328.11 and AJ000147.1. Transcript Variant: This variant (Tpm1.7, also known as variant 4) contains alternate, in-frame exons in the 3' coding region, compared to variant Tpm1.1. It encodes isoform Tpm1.7cy, also known as the TM-3 fibroblast isoform, which has a distinct C-terminus, compared to isoform Tpm1.1st. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL541623.3, BX439394.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCTCATGCCA AAGAAGAAAA CCTTAGTATG CATCAGATGC TGGATCAGAC TTTACTGGAG
TTAAACAACA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001018006.1
CDS192..1046
Misc Feature(1)192..194(+)
Misc Feature(2)210..650(+)
Misc Feature(3)333..1043(+)
Exon (1)1..305
Gene:TPM1
Gene Synonym:
Exon (2)306..431
Gene:TPM1
Gene Synonym:
Exon (3)432..565
Gene:TPM1
Gene Synonym:
Exon (4)566..683
Gene:TPM1
Gene Synonym:
Exon (5)684..754
Gene:TPM1
Gene Synonym:
Exon (6)755..830
Gene:TPM1
Gene Synonym:
Exon (7)831..893
Gene:TPM1
Gene Synonym:
Exon (8)894..963
Gene:TPM1
Gene Synonym:
Exon (9)964..1789
Gene:TPM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001018006
Organism Homo sapiens (human)
Definition Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001018006

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCTCATGCCA AAGAAGAAAA CCTTAGTATG CATCAGATGC TGGATCAGAC TTTACTGGAG
TTAAACAACA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21508
Clone ID Related Accession (Same CDS sequence) NM_001018004
Accession Version NM_001018004.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform Tpm1.6cy
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG107949.1, AC079328.11 and AJ000147.1. Transcript Variant: This variant (Tpm1.6, also known as variant 3) contains an alternate, in-frame exon in the 3' coding region, compared to variant Tpm1.1. It encodes isoform Tpm1.6cy, also known as the TM-2 fibroblast isoform, which has a distinct C-terminus, compared to isoform Tpm1.1st. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL544353.3, BX448134.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCTCATGCCA AAGAAGAAAA CCTTAGTATG CATCAGATGC TGGATCAGAC TTTACTGGAG
TTAAACAACA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001018004.1
CDS192..1046
Misc Feature(1)192..194(+)
Misc Feature(2)210..650(+)
Misc Feature(3)333..1043(+)
Exon (1)1..305
Gene:TPM1
Gene Synonym:
Exon (2)306..431
Gene:TPM1
Gene Synonym:
Exon (3)432..565
Gene:TPM1
Gene Synonym:
Exon (4)566..683
Gene:TPM1
Gene Synonym:
Exon (5)684..754
Gene:TPM1
Gene Synonym:
Exon (6)755..830
Gene:TPM1
Gene Synonym:
Exon (7)831..893
Gene:TPM1
Gene Synonym:
Exon (8)894..963
Gene:TPM1
Gene Synonym:
Exon (9)964..1789
Gene:TPM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001018004
Organism Homo sapiens (human)
Definition Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001018004

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCTCATGCCA AAGAAGAAAA CCTTAGTATG CATCAGATGC TGGATCAGAC TTTACTGGAG
TTAAACAACA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48549
Clone ID Related Accession (Same CDS sequence) NM_001301289
Accession Version NM_001301289.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 747bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform Tpm1.8cy
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079328.11. On Aug 1, 2014 this sequence version replaced gi:530406409. Transcript Variant: This variant (Tpm1.8, also known as variant 9) contains alternate in-frame exons in the 5' and 3' coding region, compared to variant Tpm1.1. It encodes isoform Tpm1.8cy, which has distinct N- and C-termini and is shorter than isoform Tpm1.1st. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301319.1, BI829132.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACTG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGAGAAAG TGGCTCATGC CAAAGAAGAA AACCTTAGTA TGCATCAGAT GCTGGATCAG
ACTTTACTGG AGTTAAACAA CATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001288218.1
CDS140..886
Misc Feature(1)173..883(+)
Misc Feature(2)173..490(+)
Exon (1)1..271
Gene:TPM1
Gene Synonym:
Exon (2)272..405
Gene:TPM1
Gene Synonym:
Exon (3)406..523
Gene:TPM1
Gene Synonym:
Exon (4)524..594
Gene:TPM1
Gene Synonym:
Exon (5)595..670
Gene:TPM1
Gene Synonym:
Exon (6)671..733
Gene:TPM1
Gene Synonym:
Exon (7)734..803
Gene:TPM1
Gene Synonym:
Exon (8)804..1629
Gene:TPM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001301289
Organism Homo sapiens (human)
Definition Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001301289

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGGA GTAGCTCGCT GGAGGCGGTG CGCAGGAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGACGCCG CTGAGGAGCG CGCGGGCACC CTGCAGCGCG AGCTGGACCA CGAGAGGAAG
CTGAGGGAGA CCGCTGAAGC CGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAAGAGTTGG ATCGTGCCCA GGAGCGTCTG GCAACAGCTT TGCAGAAGCT GGAGGAAGCT
GAGAAGGCAG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAGAGTCG AGCCCAAAAA
GATGAAGAAA AAATGGAAAT TCAGGAGATC CAACTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCCGACC GCAAATATGA AGAGGTGGCC CGTAAGCTGG TCATCATTGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACTG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCGCAG
AAGGAAGACA GATATGAGGA AGAGATCAAG GTCCTTTCCG ACAAGCTGAA GGAGGCTGAG
ACTCGGGCTG AGTTTGCGGA GAGGTCAGTA ACTAAATTGG AGAAAAGCAT TGATGACTTA
GAAGAGAAAG TGGCTCATGC CAAAGAAGAA AACCTTAGTA TGCATCAGAT GCTGGATCAG
ACTTTACTGG AGTTAAACAA CATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48542
Clone ID Related Accession (Same CDS sequence) NM_001301244
Accession Version NM_001301244.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform Tpm1.2st
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN423297.1, AY640414.1 and CD679271.1. On Jul 31, 2014 this sequence version replaced gi:530406397. Transcript Variant: This variant (Tpm1.2, also known as variant 8) contains an alternate, in-frame exon in the 5' coding region, compared to variant Tpm1.1. It encodes isoform Tpm1.2st, which has a distinct N-terminus, compared to isoform Tpm1.1st. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TACGCTCAGA AACTGAAGTA CAAAGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001288173.1
CDS192..1046
Misc Feature(1)210..650(+)
Misc Feature(2)333..1043(+)
Exon (1)1..305
Gene:TPM1
Gene Synonym:
Exon (2)306..431
Gene:TPM1
Gene Synonym:
Exon (3)432..565
Gene:TPM1
Gene Synonym:
Exon (4)566..683
Gene:TPM1
Gene Synonym:
Exon (5)684..754
Gene:TPM1
Gene Synonym:
Exon (6)755..830
Gene:TPM1
Gene Synonym:
Exon (7)831..893
Gene:TPM1
Gene Synonym:
Exon (8)894..963
Gene:TPM1
Gene Synonym:
Exon (9)964..1042
Gene:TPM1
Gene Synonym:
Exon (10)1043..1240
Gene:TPM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001301244
Organism Homo sapiens (human)
Definition Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001301244

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TACGCTCAGA AACTGAAGTA CAAAGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19397
Clone ID Related Accession (Same CDS sequence) NM_001018005
Accession Version NM_001018005.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform Tpm1.1st
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG107949.1, M19713.1 and BC007433.2. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (Tpm1.1, also known as variant 1) represents the shortest transcript. It encodes the longest isoform (Tpm1.1st), also known as the fast skeletal muscle isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M19713.1, AK299387.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TACGCTCAGA AACTGAAGTA CAAAGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001018005.1
CDS192..1046
Misc Feature(1)192..194(+)
Misc Feature(2)192..194(+)
Misc Feature(3)210..650(+)
Misc Feature(4)333..1043(+)
Misc Feature(5)348..350(+)
Misc Feature(6)372..374(+)
Misc Feature(7)426..428(+)
Misc Feature(8)513..515(+)
Misc Feature(9)711..713(+)
Misc Feature(10)807..809(+)
Misc Feature(11)1035..1037(+)
Misc Feature(12)1038..1040(+)
Exon (1)1..305
Gene:TPM1
Gene Synonym:
Exon (2)306..431
Gene:TPM1
Gene Synonym:
Exon (3)432..565
Gene:TPM1
Gene Synonym:
Exon (4)566..683
Gene:TPM1
Gene Synonym:
Exon (5)684..754
Gene:TPM1
Gene Synonym:
Exon (6)755..830
Gene:TPM1
Gene Synonym:
Exon (7)831..893
Gene:TPM1
Gene Synonym:
Exon (8)894..963
Gene:TPM1
Gene Synonym:
Exon (9)964..1042
Gene:TPM1
Gene Synonym:
Exon (10)1043..1240
Gene:TPM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001018005
Organism Homo sapiens (human)
Definition Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001018005

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TACGCTCAGA AACTGAAGTA CAAAGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16327
Clone ID Related Accession (Same CDS sequence) NM_000366
Accession Version NM_000366.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform Tpm1.5cy
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG107949.1, BC007433.2 and CN423344.1. On May 11, 2005 this sequence version replaced gi:34222222. Transcript Variant: This variant (Tpm1.5, also known as variant 5) contains an alternate, in-frame exon and uses an alternate in-frame splice site and upstream stop codon, compared to variant Tpm1.1. It encodes isoform Tpm1.5cy, which has a different C-terminus, compared to isoform Tpm1.1st. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007433.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TACGCTCAGA AACTGAAGTA CAAAGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000357.3
CDS192..1046
Misc Feature(1)192..194(+)
Misc Feature(2)210..650(+)
Misc Feature(3)333..1043(+)
Misc Feature(4)1038..1040(+)
Exon (1)1..305
Gene:TPM1
Gene Synonym:
Exon (2)306..431
Gene:TPM1
Gene Synonym:
Exon (3)432..565
Gene:TPM1
Gene Synonym:
Exon (4)566..683
Gene:TPM1
Gene Synonym:
Exon (5)684..754
Gene:TPM1
Gene Synonym:
Exon (6)755..830
Gene:TPM1
Gene Synonym:
Exon (7)831..893
Gene:TPM1
Gene Synonym:
Exon (8)894..963
Gene:TPM1
Gene Synonym:
Exon (9)964..1090
Gene:TPM1
Gene Synonym:
Exon (10)1091..1288
Gene:TPM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000366
Organism Homo sapiens (human)
Definition Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000366

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACCG AAGATGAACT GGACAAATAC
TCTGAGGCTC TCAAAGATGC CCAGGAGAAG CTGGAGCTGG CAGAGAAAAA GGCCACCGAT
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TACGCTCAGA AACTGAAGTA CAAAGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20883
Clone ID Related Accession (Same CDS sequence) NM_001018020
Accession Version NM_001018020.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform Tpm1.3sm
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG107949.1, BX458841.2, AC079328.11 and AJ000147.1. Transcript Variant: This variant (Tpm1.3, also known as variant 7) contains alternate, in-frame exons in the coding region, compared to variant Tpm1.1. It encodes isoform Tpm1.3sm, which has distinct N- and C-termini, compared to isoform Tpm1.1st. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL550928.3, BX458841.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCTCATGCCA AAGAAGAAAA CCTTAGTATG CATCAGATGC TGGATCAGAC TTTACTGGAG
TTAAACAACA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001018020.1
CDS192..1046
Misc Feature(1)192..194(+)
Misc Feature(2)210..650(+)
Misc Feature(3)333..1043(+)
Exon (1)1..305
Gene:TPM1
Gene Synonym:
Exon (2)306..431
Gene:TPM1
Gene Synonym:
Exon (3)432..565
Gene:TPM1
Gene Synonym:
Exon (4)566..683
Gene:TPM1
Gene Synonym:
Exon (5)684..754
Gene:TPM1
Gene Synonym:
Exon (6)755..830
Gene:TPM1
Gene Synonym:
Exon (7)831..893
Gene:TPM1
Gene Synonym:
Exon (8)894..963
Gene:TPM1
Gene Synonym:
Exon (9)964..1789
Gene:TPM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001018020
Organism Homo sapiens (human)
Definition Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001018020

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTC CGACAGCTGG AAGAACAATT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCTCATGCCA AAGAAGAAAA CCTTAGTATG CATCAGATGC TGGATCAGAC TTTACTGGAG
TTAAACAACA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16265
Clone ID Related Accession (Same CDS sequence) NM_001018007
Accession Version NM_001018007.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product tropomyosin alpha-1 chain isoform Tpm1.4sm
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG107949.1 and AL050179.1. Transcript Variant: This variant (Tpm1.4, also known as variant 2) contains alternate, in-frame exons in the 5' and 3' coding region, compared to variant Tpm1.1. It encodes isoform Tpm1.4sm, also known as the smooth muscle isoform, which has distinct N- and C-termini, compared to isoform Tpm1.1st. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL050179.1, AL542677.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCTCATGCCA AAGAAGAAAA CCTTAGTATG CATCAGATGC TGGATCAGAC TTTACTGGAG
TTAAACAACA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001018007.1
CDS192..1046
Misc Feature(1)192..194(+)
Misc Feature(2)210..650(+)
Misc Feature(3)333..1043(+)
Exon (1)1..305
Gene:TPM1
Gene Synonym:
Exon (2)306..431
Gene:TPM1
Gene Synonym:
Exon (3)432..565
Gene:TPM1
Gene Synonym:
Exon (4)566..683
Gene:TPM1
Gene Synonym:
Exon (5)684..754
Gene:TPM1
Gene Synonym:
Exon (6)755..830
Gene:TPM1
Gene Synonym:
Exon (7)831..893
Gene:TPM1
Gene Synonym:
Exon (8)894..963
Gene:TPM1
Gene Synonym:
Exon (9)964..1789
Gene:TPM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001018007
Organism Homo sapiens (human)
Definition Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant Tpm1.4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001018007

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAGGAGAA CGCCTTGGAT 
CGAGCTGAGC AGGCGGAGGC CGACAAGAAG GCGGCGGAAG ACAGGAGCAA GCAGCTCGAG
GAGGACATCG CGGCCAAGGA GAAGTTGCTG CGGGTGTCGG AGGACGAGCG GGACCGGGTG
CTGGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCCG CCGAAGAGGC CGCCGCCAAG
GCTGAAGCCG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA AGAGTTGGAT
CGTGCCCAGG AGCGTCTGGC AACAGCTTTG CAGAAGCTGG AGGAAGCTGA GAAGGCAGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAGAGTCGAG CCCAAAAAGA TGAAGAAAAA
ATGGAAATTC AGGAGATCCA ACTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCCGACCGC
AAATATGAAG AGGTGGCCCG TAAGCTGGTC ATCATTGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACTGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCGCAGAA GGAAGACAGA
TATGAGGAAG AGATCAAGGT CCTTTCCGAC AAGCTGAAGG AGGCTGAGAC TCGGGCTGAG
TTTGCGGAGA GGTCAGTAAC TAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCTCATGCCA AAGAAGAAAA CCTTAGTATG CATCAGATGC TGGATCAGAC TTTACTGGAG
TTAAACAACA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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