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TSC1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol TSC1
Entrez Gene ID 7248
Full Name tuberous sclerosis 1
Synonyms LAM, TSC
General protein information
Preferred Names
hamartin
Names
hamartin
tumor suppressor
tuberous sclerosis 1 protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

9

9q34

Summary This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]. lac of sum
Disorder MIM:

605284

Disorder Html: Tuberous sclerosis-1, 191100 (3); Lymphangioleiomyomatosis, 606690

mRNA and Protein(s)

mRNA Protein Name
XM_005272211 XP_005272268 hamartin isoform X1
XM_006717271 XP_006717334 hamartin isoform X1
XM_011518979 XP_011517281 hamartin isoform X1
XM_006717272 XP_006717335 hamartin isoform X2
NM_000368 NP_000359 hamartin isoform 1
NM_001162426 NP_001155898 hamartin isoform 3
NM_001162427 NP_001155899 hamartin isoform 4



Homo sapiens (human) TSC1 NP_000359.1
Pan troglodytes (chimpanzee) TSC1 XP_001168992.1
Macaca mulatta (Rhesus monkey) TSC1 XP_001103846.2
Canis lupus familiaris (dog) TSC1 XP_005625233.1
Bos taurus (cattle) TSC1 XP_005213508.1
Mus musculus (house mouse) Tsc1 NP_075025.2
Rattus norvegicus (Norway rat) Tsc1 NP_068626.1
Gallus gallus (chicken) TSC1 XP_415449.2
Danio rerio (zebrafish) tsc1a NP_956346.1
Xenopus (Silurana) tropicalis (western clawed frog) tsc1 XP_002944681.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TSC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18443
XM_005272211 PREDICTED: Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu18443
XM_006717271 PREDICTED: Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu18443
XM_011518979 PREDICTED: Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu43494 XM_006717272 PREDICTED: Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu18443
NM_000368 Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu17622 NM_001162426 Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $825.30
$1179.00
OHu17591 NM_001162427 Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $790.30
$1129.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18443
Clone ID Related Accession (Same CDS sequence) NM_000368 , XM_005272211 , XM_006717271 , XM_011518979
Accession Version XM_005272211.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hamartin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008470.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005272268.1
CDS280..3774
Misc Feature(1)283..2436(+)
Misc Feature(2)2551..3033(+)
Misc Feature(3)2575..3075(+)
Translation

Target ORF information:

RefSeq Version XM_005272211
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005272211

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18443
Clone ID Related Accession (Same CDS sequence) NM_000368 , XM_005272211 , XM_006717271 , XM_011518979
Accession Version XM_006717271.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hamartin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008470.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006717334.1
CDS215..3709
Misc Feature(1)218..2371(+)
Misc Feature(2)2486..2968(+)
Misc Feature(3)2510..3010(+)
Translation

Target ORF information:

RefSeq Version XM_006717271
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006717271

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18443
Clone ID Related Accession (Same CDS sequence) NM_000368 , XM_005272211 , XM_006717271 , XM_011518979
Accession Version XM_011518979.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hamartin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008470.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011517281.1
CDS330..3824
Misc Feature(1)333..2486(+)
Misc Feature(2)2601..3083(+)
Misc Feature(3)2625..3125(+)
Translation

Target ORF information:

RefSeq Version XM_011518979
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011518979

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43494
Clone ID Related Accession (Same CDS sequence) XM_006717272
Accession Version XM_006717272.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hamartin isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008470.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578817852. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAATT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006717335.1
CDS235..2238
Misc Feature(1)238..2235(+)
Translation

Target ORF information:

RefSeq Version XM_006717272
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006717272

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAATT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18443
Clone ID Related Accession (Same CDS sequence) NM_000368 , XM_005272211 , XM_006717271 , XM_011518979
Accession Version NM_000368.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hamartin isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC070032.1, AF013168.1, D87683.1 and N63914.1. This sequence is a reference standard in the RefSeqGene project. On Jun 23, 2009 this sequence version replaced gi:56699466. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF013168.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000359.1
CDS235..3729
Misc Feature(1)172..174(+)
Misc Feature(2)238..2391(+)
Misc Feature(3)1483..1485(+)
Misc Feature(4)1747..1749(+)
Misc Feature(5)1747..1749(+)
Misc Feature(6)1747..1749(+)
Misc Feature(7)1765..1767(+)
Misc Feature(8)1984..1986(+)
Misc Feature(9)2026..2028(+)
Misc Feature(10)2506..2988(+)
Misc Feature(11)2530..3030(+)
Misc Feature(12)3373..3375(+)
Misc Feature(13)3538..3540(+)
Exon (1)1..91
Gene:TSC1
Gene Synonym:
Exon (2)92..154
Gene:TSC1
Gene Synonym:
Exon (3)155..340
Gene:TSC1
Gene Synonym:
Exon (4)341..444
Gene:TSC1
Gene Synonym:
Exon (5)445..597
Gene:TSC1
Gene Synonym:
Exon (6)598..742
Gene:TSC1
Gene Synonym:
Exon (7)743..897
Gene:TSC1
Gene Synonym:
Exon (8)898..971
Gene:TSC1
Gene Synonym:
Exon (9)972..1147
Gene:TSC1
Gene Synonym:
Exon (10)1148..1263
Gene:TSC1
Gene Synonym:
Exon (11)1264..1375
Gene:TSC1
Gene Synonym:
Exon (12)1376..1497
Gene:TSC1
Gene Synonym:
Exon (13)1498..1567
Gene:TSC1
Gene Synonym:
Exon (14)1568..1672
Gene:TSC1
Gene Synonym:
Exon (15)1673..2231
Gene:TSC1
Gene Synonym:
Exon (16)2232..2275
Gene:TSC1
Gene Synonym:
Exon (17)2276..2442
Gene:TSC1
Gene Synonym:
Exon (18)2443..2625
Gene:TSC1
Gene Synonym:
Exon (19)2626..2736
Gene:TSC1
Gene Synonym:
Exon (20)2737..2859
Gene:TSC1
Gene Synonym:
Exon (21)2860..3047
Gene:TSC1
Gene Synonym:
Exon (22)3048..3209
Gene:TSC1
Gene Synonym:
Exon (23)3210..8616
Gene:TSC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000368
Organism Homo sapiens (human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000368

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17622
Clone ID Related Accession (Same CDS sequence) NM_001162426
Accession Version NM_001162426.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hamartin isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA726552.1, BC108668.1, AF013168.1, D87683.1 and N63914.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK308412.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001155898.1
CDS235..3726
Misc Feature(1)172..174(+)
Misc Feature(2)238..2388(+)
Misc Feature(3)1744..1746(+)
Misc Feature(4)1762..1764(+)
Misc Feature(5)2023..2025(+)
Misc Feature(6)2503..2985(+)
Misc Feature(7)2527..3027(+)
Exon (1)1..91
Gene:TSC1
Gene Synonym:
Exon (2)92..154
Gene:TSC1
Gene Synonym:
Exon (3)155..340
Gene:TSC1
Gene Synonym:
Exon (4)341..444
Gene:TSC1
Gene Synonym:
Exon (5)445..597
Gene:TSC1
Gene Synonym:
Exon (6)598..742
Gene:TSC1
Gene Synonym:
Exon (7)743..897
Gene:TSC1
Gene Synonym:
Exon (8)898..971
Gene:TSC1
Gene Synonym:
Exon (9)972..1147
Gene:TSC1
Gene Synonym:
Exon (10)1148..1263
Gene:TSC1
Gene Synonym:
Exon (11)1264..1375
Gene:TSC1
Gene Synonym:
Exon (12)1376..1494
Gene:TSC1
Gene Synonym:
Exon (13)1495..1564
Gene:TSC1
Gene Synonym:
Exon (14)1565..1669
Gene:TSC1
Gene Synonym:
Exon (15)1670..2228
Gene:TSC1
Gene Synonym:
Exon (16)2229..2272
Gene:TSC1
Gene Synonym:
Exon (17)2273..2439
Gene:TSC1
Gene Synonym:
Exon (18)2440..2622
Gene:TSC1
Gene Synonym:
Exon (19)2623..2733
Gene:TSC1
Gene Synonym:
Exon (20)2734..2856
Gene:TSC1
Gene Synonym:
Exon (21)2857..3044
Gene:TSC1
Gene Synonym:
Exon (22)3045..3206
Gene:TSC1
Gene Synonym:
Exon (23)3207..8613
Gene:TSC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001162426
Organism Homo sapiens (human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001162426

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17591
Clone ID Related Accession (Same CDS sequence) NM_001162427
Accession Version NM_001162427.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3342bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hamartin isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI463204.1, AK303030.1, AF013168.1, D87683.1 and N63914.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303030.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG ATGGACACTG ACGTCGTTGT CCTCACAACA
GGCGTCTTGG TGTTGATAAC CATGCTACCA ATGATTCCAC AGTCTGGGAA ACAGCATCTT
CTTGATTTCT TTGACATTTT TGGCCGTCTG TCATCATGGT GCCTGAAGAA ACCAGGCCAC
GTGGCGGAAG TCTATCTCGT CCATCTCCAT GCCAGTGTGT ACGCACTCTT TCATCGCCTT
TATGGAATGT ACCCTTGCAA CTTCGTCTCC TTTTTGCGTT CTCATTACAG TATGAAAGAA
AACCTGGAGA CTTTTGAAGA AGTGGTCAAG CCAATGATGG AGCATGTGCG AATTCATCCG
GAATTAGTGA CTGGATCCAA GGACCATGAA CTGGACCCTC GAAGGTGGAA GAGATTAGAA
ACTCATGATG TTGTGATCGA GTGTGCCAAA ATCTCTCTGG ATCCCACAGA AGCCTCATAT
GAAGATGGCT ATTCTGTGTC TCACCAAATC TCAGCCCGCT TTCCTCATCG TTCAGCCGAT
GTCACCACCA GCCCTTATGC TGACACACAG AATAGCTATG GGTGTGCTAC TTCTACCCCT
TACTCCACGT CTCGGCTGAT GTTGTTAAAT ATGCCAGGGC AGCTACCTCA GACTCTGAGT
TCCCCATCGA CACGGCTGAT AACTGAACCA CCACAAGCTA CTCTTTGGAG CCCATCTATG
GTTTGTGGTA TGACCACTCC TCCAACTTCT CCTGGAAATG TCCCACCTGA TCTGTCACAC
CCTTACAGTA AAGTCTTTGG TACAACTGCA GGTGGAAAAG GAACTCCTCT GGGAACCCCA
GCAACCTCTC CTCCTCCAGC CCCACTCTGT CATTCGGATG ACTACGTGCA CATTTCACTC
CCCCAGGCCA CAGTCACACC CCCCAGGAAG GAAGAGAGAA TGGATTCTGC AAGACCATGT
CTACACAGAC AACACCATCT TCTGAATGAC AGAGGATCAG AAGAGCCACC TGGCAGCAAA
GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT
AGTATTGAAA AAGATAAAGA AGAAGCTGCA ATATCTAGAG AACTTTCTGA GATCACCACA
GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA GGCTTTGACT CTCCCTTTTA CCGAGACAGT
CTCCCAGGTT CTCAGCGGAA GACCCACTCG GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG
AACCCTGAGC CTTTACACTC CTCCCTGGAC AAGCTTGGGC CTGACACACC AAAGCAAGCC
TTTACTCCCA TAGACCTGCC CTGCGGCAGT GCTGATGAAA GCCCTGCGGG AGACAGGGAA
TGCCAGACTT CTTTGGAGAC CAGTATCTTC ACTCCCAGTC CTTGTAAAAT TCCACCTCCG
ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT CCCCCGTATG ATCATCTTTT TGAGGTGGCA
TTGCCAAAGA CAGCCCATCA TTTTGTCATC AGGAAGACTG AGGAGCTGTT AAAGAAAGCA
AAAGGAAACA CAGAGGAAGA TGGTGTGCCC TCTACCTCCC CAATGGAAGT GCTGGACAGA
CTGATACAGC AGGGAGCAGA CGCGCACAGC AAGGAGCTGA ACAAGTTGCC TTTACCCAGC
AAGTCTGTCG ACTGGACCCA CTTTGGAGGC TCTCCTCCTT CAGATGAGAT CCGCACCCTC
CGAGACCAGT TGCTTTTACT GCACAACCAG TTACTCTATG AGCGTTTTAA GAGGCAGCAG
CATGCCCTCC GGAACAGGCG GCTCCTCCGC AAGGTGATCA AAGCAGCAGC TCTGGAGGAA
CATAATGCTG CCATGAAAGA TCAGTTGAAG TTACAAGAGA AGGACATCCA GATGTGGAAG
GTTAGTCTGC AGAAAGAACA AGCTAGATAC AATCAGCTCC AGGAGCAGCG TGACACTATG
GTAACCAAGC TCCACAGCCA GATCAGACAG CTGCAGCATG ACCGAGAGGA ATTCTACAAC
CAGAGCCAGG AATTACAGAC GAAGCTGGAG GACTGCAGGA ACATGATTGC GGAGCTGCGG
ATAGAACTGA AGAAGGCCAA CAACAAGGTG TGTCACACTG AGCTGCTGCT CAGTCAGGTT
TCCCAAAAGC TCTCAAACAG TGAGTCGGTC CAGCAGCAGA TGGAGTTCTT GAACAGGCAG
CTGTTGGTTC TTGGGGAGGT CAACGAGCTC TATTTGGAAC AACTGCAGAA CAAGCACTCA
GATACCACAA AGGAAGTAGA AATGATGAAA GCCGCCTATC GGAAAGAGCT AGAAAAAAAC
AGAAGCCATG TTCTCCAGCA GACTCAGAGG CTTGATACCT CCCAAAAACG GATTTTGGAA
CTGGAATCTC ACCTGGCCAA GAAAGACCAC CTTCTTTTGG AACAGAAGAA ATATCTAGAG
GATGTCAAAC TCCAGGCAAG AGGACAGCTG CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG
AAAAGGATAA CCCAGGTGTT TGAATTGGAG ATCTTAGATT TATATGGCAG GTTGGAGAAA
GATGGCCTCC TGAAAAAACT TGAAGAAGAA AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA
AGGCTTGACT GTTGTAATGA CGGGTGCTCA GATTCCATGG TAGGGCACAA TGAAGAGGCA
TCTGGCCACA ACGGTGAGAC CAAGACCCCC AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA
AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC AGCAGCGAGC TTTCTACCCC AGAGAAACCC
CCACACCAGA GGGCAGGCCC ATTCAGCAGT CGGTGGGAGA CGACTATGGG AGAAGCGTCT
GCCAGCATCC CCACCACTGT GGGCTCACTT CCCAGTTCAA AAAGCTTCCT GGGTATGAAG
GCTCGAGAGT TATTTCGTAA TAAGAGCGAG AGCCAGTGTG ATGAGGACGG CATGACCAGT
AGCCTTTCTG AGAGCCTAAA GACAGAACTG GGCAAAGACT TGGGTGTGGA AGCCAAGATT
CCCCTGAACC TAGATGGCCC TCACCCGTCT CCCCCGACCC CGGACAGTGT TGGACAGCTA
CATATCATGG ACTACAATGA GACTCATCAT GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001155899.1
CDS235..3576
Misc Feature(1)172..174(+)
Misc Feature(2)238..2238(+)
Misc Feature(3)2353..2835(+)
Misc Feature(4)2377..2877(+)
Exon (1)1..91
Gene:TSC1
Gene Synonym:
Exon (2)92..154
Gene:TSC1
Gene Synonym:
Exon (3)155..340
Gene:TSC1
Gene Synonym:
Exon (4)341..444
Gene:TSC1
Gene Synonym:
Exon (5)445..589
Gene:TSC1
Gene Synonym:
Exon (6)590..744
Gene:TSC1
Gene Synonym:
Exon (7)745..818
Gene:TSC1
Gene Synonym:
Exon (8)819..994
Gene:TSC1
Gene Synonym:
Exon (9)995..1110
Gene:TSC1
Gene Synonym:
Exon (10)1111..1222
Gene:TSC1
Gene Synonym:
Exon (11)1223..1344
Gene:TSC1
Gene Synonym:
Exon (12)1345..1414
Gene:TSC1
Gene Synonym:
Exon (13)1415..1519
Gene:TSC1
Gene Synonym:
Exon (14)1520..2078
Gene:TSC1
Gene Synonym:
Exon (15)2079..2122
Gene:TSC1
Gene Synonym:
Exon (16)2123..2289
Gene:TSC1
Gene Synonym:
Exon (17)2290..2472
Gene:TSC1
Gene Synonym:
Exon (18)2473..2583
Gene:TSC1
Gene Synonym:
Exon (19)2584..2706
Gene:TSC1
Gene Synonym:
Exon (20)2707..2894
Gene:TSC1
Gene Synonym:
Exon (21)2895..3056
Gene:TSC1
Gene Synonym:
Exon (22)3057..8463
Gene:TSC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001162427
Organism Homo sapiens (human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001162427

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG ATGGACACTG ACGTCGTTGT CCTCACAACA
GGCGTCTTGG TGTTGATAAC CATGCTACCA ATGATTCCAC AGTCTGGGAA ACAGCATCTT
CTTGATTTCT TTGACATTTT TGGCCGTCTG TCATCATGGT GCCTGAAGAA ACCAGGCCAC
GTGGCGGAAG TCTATCTCGT CCATCTCCAT GCCAGTGTGT ACGCACTCTT TCATCGCCTT
TATGGAATGT ACCCTTGCAA CTTCGTCTCC TTTTTGCGTT CTCATTACAG TATGAAAGAA
AACCTGGAGA CTTTTGAAGA AGTGGTCAAG CCAATGATGG AGCATGTGCG AATTCATCCG
GAATTAGTGA CTGGATCCAA GGACCATGAA CTGGACCCTC GAAGGTGGAA GAGATTAGAA
ACTCATGATG TTGTGATCGA GTGTGCCAAA ATCTCTCTGG ATCCCACAGA AGCCTCATAT
GAAGATGGCT ATTCTGTGTC TCACCAAATC TCAGCCCGCT TTCCTCATCG TTCAGCCGAT
GTCACCACCA GCCCTTATGC TGACACACAG AATAGCTATG GGTGTGCTAC TTCTACCCCT
TACTCCACGT CTCGGCTGAT GTTGTTAAAT ATGCCAGGGC AGCTACCTCA GACTCTGAGT
TCCCCATCGA CACGGCTGAT AACTGAACCA CCACAAGCTA CTCTTTGGAG CCCATCTATG
GTTTGTGGTA TGACCACTCC TCCAACTTCT CCTGGAAATG TCCCACCTGA TCTGTCACAC
CCTTACAGTA AAGTCTTTGG TACAACTGCA GGTGGAAAAG GAACTCCTCT GGGAACCCCA
GCAACCTCTC CTCCTCCAGC CCCACTCTGT CATTCGGATG ACTACGTGCA CATTTCACTC
CCCCAGGCCA CAGTCACACC CCCCAGGAAG GAAGAGAGAA TGGATTCTGC AAGACCATGT
CTACACAGAC AACACCATCT TCTGAATGAC AGAGGATCAG AAGAGCCACC TGGCAGCAAA
GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT
AGTATTGAAA AAGATAAAGA AGAAGCTGCA ATATCTAGAG AACTTTCTGA GATCACCACA
GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA GGCTTTGACT CTCCCTTTTA CCGAGACAGT
CTCCCAGGTT CTCAGCGGAA GACCCACTCG GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG
AACCCTGAGC CTTTACACTC CTCCCTGGAC AAGCTTGGGC CTGACACACC AAAGCAAGCC
TTTACTCCCA TAGACCTGCC CTGCGGCAGT GCTGATGAAA GCCCTGCGGG AGACAGGGAA
TGCCAGACTT CTTTGGAGAC CAGTATCTTC ACTCCCAGTC CTTGTAAAAT TCCACCTCCG
ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT CCCCCGTATG ATCATCTTTT TGAGGTGGCA
TTGCCAAAGA CAGCCCATCA TTTTGTCATC AGGAAGACTG AGGAGCTGTT AAAGAAAGCA
AAAGGAAACA CAGAGGAAGA TGGTGTGCCC TCTACCTCCC CAATGGAAGT GCTGGACAGA
CTGATACAGC AGGGAGCAGA CGCGCACAGC AAGGAGCTGA ACAAGTTGCC TTTACCCAGC
AAGTCTGTCG ACTGGACCCA CTTTGGAGGC TCTCCTCCTT CAGATGAGAT CCGCACCCTC
CGAGACCAGT TGCTTTTACT GCACAACCAG TTACTCTATG AGCGTTTTAA GAGGCAGCAG
CATGCCCTCC GGAACAGGCG GCTCCTCCGC AAGGTGATCA AAGCAGCAGC TCTGGAGGAA
CATAATGCTG CCATGAAAGA TCAGTTGAAG TTACAAGAGA AGGACATCCA GATGTGGAAG
GTTAGTCTGC AGAAAGAACA AGCTAGATAC AATCAGCTCC AGGAGCAGCG TGACACTATG
GTAACCAAGC TCCACAGCCA GATCAGACAG CTGCAGCATG ACCGAGAGGA ATTCTACAAC
CAGAGCCAGG AATTACAGAC GAAGCTGGAG GACTGCAGGA ACATGATTGC GGAGCTGCGG
ATAGAACTGA AGAAGGCCAA CAACAAGGTG TGTCACACTG AGCTGCTGCT CAGTCAGGTT
TCCCAAAAGC TCTCAAACAG TGAGTCGGTC CAGCAGCAGA TGGAGTTCTT GAACAGGCAG
CTGTTGGTTC TTGGGGAGGT CAACGAGCTC TATTTGGAAC AACTGCAGAA CAAGCACTCA
GATACCACAA AGGAAGTAGA AATGATGAAA GCCGCCTATC GGAAAGAGCT AGAAAAAAAC
AGAAGCCATG TTCTCCAGCA GACTCAGAGG CTTGATACCT CCCAAAAACG GATTTTGGAA
CTGGAATCTC ACCTGGCCAA GAAAGACCAC CTTCTTTTGG AACAGAAGAA ATATCTAGAG
GATGTCAAAC TCCAGGCAAG AGGACAGCTG CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG
AAAAGGATAA CCCAGGTGTT TGAATTGGAG ATCTTAGATT TATATGGCAG GTTGGAGAAA
GATGGCCTCC TGAAAAAACT TGAAGAAGAA AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA
AGGCTTGACT GTTGTAATGA CGGGTGCTCA GATTCCATGG TAGGGCACAA TGAAGAGGCA
TCTGGCCACA ACGGTGAGAC CAAGACCCCC AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA
AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC AGCAGCGAGC TTTCTACCCC AGAGAAACCC
CCACACCAGA GGGCAGGCCC ATTCAGCAGT CGGTGGGAGA CGACTATGGG AGAAGCGTCT
GCCAGCATCC CCACCACTGT GGGCTCACTT CCCAGTTCAA AAAGCTTCCT GGGTATGAAG
GCTCGAGAGT TATTTCGTAA TAAGAGCGAG AGCCAGTGTG ATGAGGACGG CATGACCAGT
AGCCTTTCTG AGAGCCTAAA GACAGAACTG GGCAAAGACT TGGGTGTGGA AGCCAAGATT
CCCCTGAACC TAGATGGCCC TCACCCGTCT CCCCCGACCC CGGACAGTGT TGGACAGCTA
CATATCATGG ACTACAATGA GACTCATCAT GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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