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TUBA4A cDNA ORF clone, Homo sapiens (human)

Gene Symbol TUBA4A
Entrez Gene ID 7277
Full Name tubulin, alpha 4a
Synonyms ALS22, H2-ALPHA, TUBA1
General protein information
Preferred Names
tubulin alpha-4A chain
Names
tubulin alpha-4A chain
tubulin H2-alpha
tubulin alpha-1 chain
tubulin, alpha 1 (testis specific)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q35

Summary Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]. lac of sum
Disorder MIM:

191110

Disorder Html:





The following TUBA4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TUBA4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu09800 XM_005246832 PREDICTED: Homo sapiens tubulin, alpha 4a (TUBA4A), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu21787 NM_006000 Homo sapiens tubulin, alpha 4a (TUBA4A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.30
$379.00
OHu09800 NM_001278552 Homo sapiens tubulin, alpha 4a (TUBA4A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu09800
Accession Version XM_005246832.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1302bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tubulin alpha-4A chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGCAATG CCTGCTGGGA GCTCTATTGC TTGGAACATG GGATTCAGCC TGATGGGCAG 
ATGCCCAGTG ACAAGACCAT TGGTGGAGGG GACGACTCCT TCACCACCTT CTTCTGTGAA
ACTGGTGCTG GAAAACACGT ACCCCGGGCA GTTTTTGTGG ATCTGGAGCC TACGGTCATT
GATGAGATCC GAAATGGCCC ATACCGACAG CTCTTCCACC CAGAGCAGCT CATCACTGGG
AAAGAGGATG CTGCCAACAA CTATGCCCGT GGTCACTATA CCATTGGCAA GGAGATCATT
GACCCAGTGC TGGATCGGAT CCGCAAGCTG TCTGACCAGT GCACAGGACT TCAGGGCTTC
CTGGTGTTCC ACAGCTTTGG TGGGGGCACT GGCTCTGGCT TCACCTCACT CCTGATGGAG
CGGCTCTCTG TTGACTATGG CAAGAAATCC AAGCTGGAAT TCTCCATCTA CCCAGCCCCC
CAGGTGTCTA CAGCCGTGGT CGAGCCCTAC AACTCTATCC TGACCACCCA CACCACCCTG
GAGCACTCAG ACTGTGCCTT CATGGTGGAC AACGAAGCAA TCTATGACAT CTGCCGCCGC
AACCTAGACA TCGAGCGCCC AACCTACACC AACCTCAATC GCCTCATTAG CCAAATTGTC
TCCTCCATCA CAGCTTCTCT GCGCTTTGAC GGGGCCCTCA ATGTGGACCT GACAGAGTTC
CAGACCAACC TGGTGCCCTA CCCTCGCATC CACTTCCCCC TGGCCACCTA TGCACCAGTC
ATCTCTGCAG AAAAGGCATA CCACGAGCAG CTGTCGGTGG CAGAGATCAC CAATGCCTGC
TTTGAGCCTG CCAACCAGAT GGTAAAGTGT GATCCCCGGC ACGGCAAGTA CATGGCCTGC
TGCCTGCTGT ACCGTGGAGA TGTGGTGCCC AAGGATGTCA ACGCTGCCAT TGCCGCCATC
AAGACCAAGC GCAGCATTCA GTTTGTGGAC TGGTGCCCCA CAGGCTTCAA GGTTGGTATC
AACTACCAGC CTCCCACTGT GGTGCCTGGG GGTGACCTGG CCAAGGTGCA GCGTGCCGTG
TGCATGCTGA GCAACACGAC CGCCATCGCC GAGGCCTGGG CCCGCCTGGA CCACAAGTTC
GACCTGATGT ATGCCAAGAG GGCGTTTGTG CACTGGTATG TGGGTGAGGG CATGGAGGAG
GGTGAGTTCT CCGAGGCCCG TGAGGATATG GCTGCCCTGG AGAAGGATTA TGAGGAGGTG
GGCATCGACT CCTATGAGGA CGAGGATGAG GGAGAAGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005246889.1
CDS189..1490
Misc Feature(1)189..1487(+)
Misc Feature(2)189..1448(+)
Misc Feature(3)354..827(+)
Misc Feature(4)357..815(+)
Misc Feature(5)738..1199(+)
Translation
Position Chain Variation Link
17 17 c, g dbSNP:572110487
46 46 c, g dbSNP:376146379
78 78 a, c dbSNP:181713232
88 88 a, c dbSNP:541456980
104 104 g, t dbSNP:572432268
127 127 a, g dbSNP:558936162
138 138 a, c dbSNP:545321458
173 173 a, g dbSNP:781322731
185 185 c, t dbSNP:138241750
192 192 a, g dbSNP:751783740
200 200 c, t dbSNP:777029118
209 209 a, g dbSNP:141429751
215 215 c, t dbSNP:537411926
218 218 c, g dbSNP:753230693
227 227 c, t dbSNP:145576984
230 230 a, g dbSNP:373338390
248 248 c, g dbSNP:368969801
252 252 c, t dbSNP:766075261
256 256 c, g dbSNP:760363903
265 265 a, c dbSNP:772807683
266 266 c, t dbSNP:535391274
268 268 c, t dbSNP:201865832
271 271 g, t dbSNP:774232761
273 273 a, g dbSNP:768583053
277 277 c, g dbSNP:745889662
278 278 g, t dbSNP:11556348
281 281 c, t dbSNP:140319726
282 282 a, g, t dbSNP:757156202
312 312 c, g dbSNP:747219812
317 317 c, t dbSNP:777902454
322 322 a, g dbSNP:758743744
325 325 a, g dbSNP:748558025
326 326 c, t dbSNP:376398028
329 329 a, g dbSNP:755558869
346 346 c, t dbSNP:372756767
350 350 a, t dbSNP:765910022
354 354 a, g dbSNP:755620655
362 362 a, g dbSNP:567717225
378 378 c, t dbSNP:780475810
387 387 c, t dbSNP:755609946
392 392 a, c dbSNP:749906171
393 393 c, t dbSNP:757590679
394 394 a, g dbSNP:756888946
397 397 a, g dbSNP:751277119
398 398 a, g dbSNP:763842483
401 401 c, g dbSNP:755752894
413 413 a, g dbSNP:45571337
446 446 c, t dbSNP:752628707
455 455 a, c, t dbSNP:11556347
457 457 a, g dbSNP:760745277
467 467 c, t dbSNP:199579096
471 471 a, g dbSNP:200651096
472 472 c, t dbSNP:201172087
475 475 a, g dbSNP:201649283
480 480 -, gag dbSNP:773787054
485 485 c, t dbSNP:531687027
492 492 c, t dbSNP:762123008
494 494 a, g dbSNP:774757044
504 504 c, t dbSNP:769120095
510 510 c, t dbSNP:749694106
511 511 a, g dbSNP:780543647
512 512 c, t dbSNP:562650415
515 515 a, g dbSNP:746442903
524 524 c, g dbSNP:775703836
542 542 a, g dbSNP:770260351
551 551 a, g dbSNP:372079932
552 552 c, g dbSNP:781745194
570 570 c, g dbSNP:770376188
575 575 a, c dbSNP:746551411
576 576 a, c dbSNP:730880029
584 584 c, t dbSNP:777343840
608 608 a, g dbSNP:368461384
610 610 a, g dbSNP:752457075
625 625 a, g dbSNP:778571547
626 626 c, t dbSNP:754843931
646 646 a, g dbSNP:753697044
665 665 c, t dbSNP:374107920
683 683 c, t dbSNP:766444250
684 684 a, g dbSNP:757373635
689 689 a, c, g, t dbSNP:575169424
690 690 a, g dbSNP:775821469
707 707 c, t dbSNP:765492925
728 728 a, g dbSNP:759963297
731 731 a, g dbSNP:777080257
755 755 g, t dbSNP:771428804
761 761 c, t dbSNP:556632744
762 762 a, g dbSNP:772698843
774 774 g, t dbSNP:771641172
783 783 c, t dbSNP:747728810
784 784 a, g dbSNP:778676398
786 786 c, t dbSNP:730880028
787 787 a, g dbSNP:371074506
791 791 c, t dbSNP:749141733
792 792 a, c dbSNP:80231183
797 797 c, t dbSNP:141146222
800 800 c, t dbSNP:756105407
803 803 a, g dbSNP:750452564
805 805 a, g dbSNP:199826343
812 812 a, c dbSNP:758550918
821 821 c, t dbSNP:752808482
862 862 c, t dbSNP:765525084
867 867 c, t dbSNP:370939488
870 870 c, t dbSNP:199881197
871 871 a, g dbSNP:766789413
872 872 c, t dbSNP:190009503
878 878 c, t dbSNP:773752934
890 890 c, t dbSNP:567800140
921 921 a, g dbSNP:747715758
924 924 c, t dbSNP:147946711
932 932 g, t dbSNP:768360652
933 933 c, t dbSNP:749005381
934 934 a, g dbSNP:141069951
943 943 c, t dbSNP:756014119
944 944 c, t dbSNP:745777122
958 958 a, g dbSNP:781297152
964 964 c, t dbSNP:758365970
965 965 a, c dbSNP:752825007
969 969 a, g dbSNP:377319362
983 983 a, c, g dbSNP:754128746
985 985 c, t dbSNP:766703407
992 992 c, t dbSNP:149475041
1004 1004 a, g dbSNP:750882247
1006 1006 g, t dbSNP:768101021
1008 1008 g, t dbSNP:548026518
1016 1016 c, t dbSNP:773926584
1034 1034 c, g dbSNP:768116035
1038 1038 a, g dbSNP:762662180
1043 1043 c, t dbSNP:775330235
1046 1046 a, g dbSNP:769660058
1052 1052 a, g dbSNP:141922502
1065 1065 c, t dbSNP:780921701
1066 1066 a, g dbSNP:770910730
1070 1070 c, t dbSNP:746979509
1087 1087 a, g dbSNP:779048233
1101 1101 c, t dbSNP:730880025
1102 1102 a, g dbSNP:730880026
1117 1117 c, g dbSNP:754040899
1130 1130 c, t dbSNP:372891488
1131 1131 a, g dbSNP:369027314
1142 1142 c, t dbSNP:147351423
1143 1143 a, g dbSNP:750792560
1144 1144 c, t dbSNP:767856035
1153 1153 c, g dbSNP:762376608
1158 1158 c, t dbSNP:752044060
1168 1168 a, g dbSNP:763637343
1170 1170 a, t dbSNP:762574079
1201 1201 c, t dbSNP:775239569
1208 1208 a, c dbSNP:759049795
1218 1218 c, t dbSNP:769571931
1227 1227 a, g dbSNP:759343956
1232 1232 a, g dbSNP:776470776
1237 1237 a, g dbSNP:770701335
1256 1256 a, g dbSNP:746937350
1260 1260 c, t dbSNP:551859540
1261 1261 a, g dbSNP:768677078
1265 1265 a, c, t dbSNP:531647997
1266 1266 a, g dbSNP:377431750
1283 1283 c, t dbSNP:746135853
1285 1285 c, t dbSNP:781587642
1286 1286 a, g dbSNP:757609748
1289 1289 c, t dbSNP:373071036
1290 1290 a, g dbSNP:368743618
1295 1295 c, t dbSNP:757911669
1298 1298 c, t dbSNP:752266046
1299 1299 a, g dbSNP:764895997
1328 1328 c, t dbSNP:562895831
1334 1334 g, t dbSNP:549035311
1336 1336 c, t dbSNP:766177312
1351 1351 c, t dbSNP:760414026
1352 1352 a, g dbSNP:750069877
1357 1357 g, t dbSNP:773161555
1363 1363 a, g dbSNP:730880027
1379 1379 a, c dbSNP:772002925
1388 1388 g, t dbSNP:749310004
1400 1400 c, t dbSNP:199590938
1401 1401 g, t dbSNP:769899863
1406 1406 a, c dbSNP:746080269
1423 1423 c, g dbSNP:781292239
1426 1426 c, t dbSNP:757601862
1427 1427 g, t dbSNP:14760
1429 1429 a, c, g dbSNP:778363691
1430 1430 a, c, g dbSNP:201092237
1435 1435 a, g dbSNP:753320398
1436 1436 a, c, t dbSNP:754497882
1452 1452 a, t dbSNP:753485163
1454 1454 c, g, t dbSNP:760451884
1462 1462 a, g dbSNP:376008810
1467 1467 a, g dbSNP:772897588
1472 1472 a, g dbSNP:1063394
1481 1481 a, c dbSNP:767359528
1498 1498 a, g dbSNP:142720001
1505 1505 a, g dbSNP:774319060
1513 1513 a, g dbSNP:769814111
1517 1517 c, t dbSNP:369629125
1518 1518 a, g dbSNP:776842527
1522 1522 a, g dbSNP:771067583
1524 1524 -, t dbSNP:765926994
1524 1524 c, t dbSNP:747310177
1525 1525 g, t dbSNP:778275983
1533 1533 c, t dbSNP:772607875
1537 1537 a, c, t dbSNP:376317489
1538 1538 a, g dbSNP:11556349

Target ORF information:

RefSeq Version XM_005246832
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tubulin, alpha 4a (TUBA4A), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005246832

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGCAATG CCTGCTGGGA GCTCTATTGC TTGGAACATG GGATTCAGCC TGATGGGCAG 
ATGCCCAGTG ACAAGACCAT TGGTGGAGGG GACGACTCCT TCACCACCTT CTTCTGTGAA
ACTGGTGCTG GAAAACACGT ACCCCGGGCA GTTTTTGTGG ATCTGGAGCC TACGGTCATT
GATGAGATCC GAAATGGCCC ATACCGACAG CTCTTCCACC CAGAGCAGCT CATCACTGGG
AAAGAGGATG CTGCCAACAA CTATGCCCGT GGTCACTATA CCATTGGCAA GGAGATCATT
GACCCAGTGC TGGATCGGAT CCGCAAGCTG TCTGACCAGT GCACAGGACT TCAGGGCTTC
CTGGTGTTCC ACAGCTTTGG TGGGGGCACT GGCTCTGGCT TCACCTCACT CCTGATGGAG
CGGCTCTCTG TTGACTATGG CAAGAAATCC AAGCTGGAAT TCTCCATCTA CCCAGCCCCC
CAGGTGTCTA CAGCCGTGGT CGAGCCCTAC AACTCTATCC TGACCACCCA CACCACCCTG
GAGCACTCAG ACTGTGCCTT CATGGTGGAC AACGAAGCAA TCTATGACAT CTGCCGCCGC
AACCTAGACA TCGAGCGCCC AACCTACACC AACCTCAATC GCCTCATTAG CCAAATTGTC
TCCTCCATCA CAGCTTCTCT GCGCTTTGAC GGGGCCCTCA ATGTGGACCT GACAGAGTTC
CAGACCAACC TGGTGCCCTA CCCTCGCATC CACTTCCCCC TGGCCACCTA TGCACCAGTC
ATCTCTGCAG AAAAGGCATA CCACGAGCAG CTGTCGGTGG CAGAGATCAC CAATGCCTGC
TTTGAGCCTG CCAACCAGAT GGTAAAGTGT GATCCCCGGC ACGGCAAGTA CATGGCCTGC
TGCCTGCTGT ACCGTGGAGA TGTGGTGCCC AAGGATGTCA ACGCTGCCAT TGCCGCCATC
AAGACCAAGC GCAGCATTCA GTTTGTGGAC TGGTGCCCCA CAGGCTTCAA GGTTGGTATC
AACTACCAGC CTCCCACTGT GGTGCCTGGG GGTGACCTGG CCAAGGTGCA GCGTGCCGTG
TGCATGCTGA GCAACACGAC CGCCATCGCC GAGGCCTGGG CCCGCCTGGA CCACAAGTTC
GACCTGATGT ATGCCAAGAG GGCGTTTGTG CACTGGTATG TGGGTGAGGG CATGGAGGAG
GGTGAGTTCT CCGAGGCCCG TGAGGATATG GCTGCCCTGG AGAAGGATTA TGAGGAGGTG
GGCATCGACT CCTATGAGGA CGAGGATGAG GGAGAAGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21787
Accession Version NM_006000.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tubulin alpha-4A chain isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP346611.1 and AK054731.1. This sequence is a reference standard in the RefSeqGene project. On Jun 21, 2013 this sequence version replaced gi:17921988. Summary: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK054731.1, BC009238.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGAAT GCATCTCAGT CCACGTGGGG CAGGCAGGTG TCCAGATGGG CAATGCCTGC 
TGGGAGCTCT ATTGCTTGGA ACATGGGATT CAGCCTGATG GGCAGATGCC CAGTGACAAG
ACCATTGGTG GAGGGGACGA CTCCTTCACC ACCTTCTTCT GTGAAACTGG TGCTGGAAAA
CACGTACCCC GGGCAGTTTT TGTGGATCTG GAGCCTACGG TCATTGATGA GATCCGAAAT
GGCCCATACC GACAGCTCTT CCACCCAGAG CAGCTCATCA CTGGGAAAGA GGATGCTGCC
AACAACTATG CCCGTGGTCA CTATACCATT GGCAAGGAGA TCATTGACCC AGTGCTGGAT
CGGATCCGCA AGCTGTCTGA CCAGTGCACA GGACTTCAGG GCTTCCTGGT GTTCCACAGC
TTTGGTGGGG GCACTGGCTC TGGCTTCACC TCACTCCTGA TGGAGCGGCT CTCTGTTGAC
TATGGCAAGA AATCCAAGCT GGAATTCTCC ATCTACCCAG CCCCCCAGGT GTCTACAGCC
GTGGTCGAGC CCTACAACTC TATCCTGACC ACCCACACCA CCCTGGAGCA CTCAGACTGT
GCCTTCATGG TGGACAACGA AGCAATCTAT GACATCTGCC GCCGCAACCT AGACATCGAG
CGCCCAACCT ACACCAACCT CAATCGCCTC ATTAGCCAAA TTGTCTCCTC CATCACAGCT
TCTCTGCGCT TTGACGGGGC CCTCAATGTG GACCTGACAG AGTTCCAGAC CAACCTGGTG
CCCTACCCTC GCATCCACTT CCCCCTGGCC ACCTATGCAC CAGTCATCTC TGCAGAAAAG
GCATACCACG AGCAGCTGTC GGTGGCAGAG ATCACCAATG CCTGCTTTGA GCCTGCCAAC
CAGATGGTAA AGTGTGATCC CCGGCACGGC AAGTACATGG CCTGCTGCCT GCTGTACCGT
GGAGATGTGG TGCCCAAGGA TGTCAACGCT GCCATTGCCG CCATCAAGAC CAAGCGCAGC
ATTCAGTTTG TGGACTGGTG CCCCACAGGC TTCAAGGTTG GTATCAACTA CCAGCCTCCC
ACTGTGGTGC CTGGGGGTGA CCTGGCCAAG GTGCAGCGTG CCGTGTGCAT GCTGAGCAAC
ACGACCGCCA TCGCCGAGGC CTGGGCCCGC CTGGACCACA AGTTCGACCT GATGTATGCC
AAGAGGGCGT TTGTGCACTG GTATGTGGGT GAGGGCATGG AGGAGGGTGA GTTCTCCGAG
GCCCGTGAGG ATATGGCTGC CCTGGAGAAG GATTATGAGG AGGTGGGCAT CGACTCCTAT
GAGGACGAGG ATGAGGGAGA AGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_005991.1
CDS89..1435
Misc Feature(1)65..67(+)
Misc Feature(2)89..1432(+)
Misc Feature(3)92..1393(+)
Misc Feature(4)92..1144(+)
Misc Feature(5)119..772(+)
Misc Feature(6)206..208(+)
Misc Feature(7)206..208(+)
Misc Feature(8)230..232(+)
Misc Feature(9)230..232(+)
Misc Feature(10)302..760(+)
Misc Feature(11)335..337(+)
Misc Feature(12)902..904(+)
Misc Feature(13)932..934(+)
Misc Feature(14)1157..1159(+)
Misc Feature(15)1382..1384(+)
Misc Feature(16)1382..1384(+)
Misc Feature(17)1403..1405(+)
Exon (1)1..91
Gene:TUBA4A
Gene Synonym:
Exon (2)92..314
Gene:TUBA4A
Gene Synonym:
Exon (3)315..463
Gene:TUBA4A
Gene Synonym:
Exon (4)464..2076
Gene:TUBA4A
Gene Synonym:
Translation
Position Chain Variation Link
15 15 c, g dbSNP:528746051
48 48 a, g dbSNP:774963279
56 56 c, g dbSNP:559586186
57 57 a, t dbSNP:546010501
68 68 c, g dbSNP:200129330
79 79 c, t dbSNP:377724136
86 86 a, c dbSNP:200615683
118 118 a, g dbSNP:781322731
130 130 c, t dbSNP:138241750
137 137 a, g dbSNP:751783740
145 145 c, t dbSNP:777029118
154 154 a, g dbSNP:141429751
160 160 c, t dbSNP:537411926
163 163 c, g dbSNP:753230693
172 172 c, t dbSNP:145576984
175 175 a, g dbSNP:373338390
193 193 c, g dbSNP:368969801
197 197 c, t dbSNP:766075261
201 201 c, g dbSNP:760363903
210 210 a, c dbSNP:772807683
211 211 c, t dbSNP:535391274
213 213 c, t dbSNP:201865832
216 216 g, t dbSNP:774232761
218 218 a, g dbSNP:768583053
222 222 c, g dbSNP:745889662
223 223 g, t dbSNP:11556348
226 226 c, t dbSNP:140319726
227 227 a, g, t dbSNP:757156202
257 257 c, g dbSNP:747219812
262 262 c, t dbSNP:777902454
267 267 a, g dbSNP:758743744
270 270 a, g dbSNP:748558025
271 271 c, t dbSNP:376398028
274 274 a, g dbSNP:755558869
291 291 c, t dbSNP:372756767
295 295 a, t dbSNP:765910022
299 299 a, g dbSNP:755620655
307 307 a, g dbSNP:567717225
323 323 c, t dbSNP:780475810
332 332 c, t dbSNP:755609946
337 337 a, c dbSNP:749906171
338 338 c, t dbSNP:757590679
339 339 a, g dbSNP:756888946
342 342 a, g dbSNP:751277119
343 343 a, g dbSNP:763842483
346 346 c, g dbSNP:755752894
358 358 a, g dbSNP:45571337
391 391 c, t dbSNP:752628707
400 400 a, c, t dbSNP:11556347
402 402 a, g dbSNP:760745277
412 412 c, t dbSNP:199579096
416 416 a, g dbSNP:200651096
417 417 c, t dbSNP:201172087
420 420 a, g dbSNP:201649283
425 425 -, gag dbSNP:773787054
430 430 c, t dbSNP:531687027
437 437 c, t dbSNP:762123008
439 439 a, g dbSNP:774757044
449 449 c, t dbSNP:769120095
455 455 c, t dbSNP:749694106
456 456 a, g dbSNP:780543647
457 457 c, t dbSNP:562650415
460 460 a, g dbSNP:746442903
469 469 c, g dbSNP:775703836
487 487 a, g dbSNP:770260351
496 496 a, g dbSNP:372079932
497 497 c, g dbSNP:781745194
515 515 c, g dbSNP:770376188
520 520 a, c dbSNP:746551411
521 521 a, c dbSNP:730880029
529 529 c, t dbSNP:777343840
553 553 a, g dbSNP:368461384
555 555 a, g dbSNP:752457075
570 570 a, g dbSNP:778571547
571 571 c, t dbSNP:754843931
591 591 a, g dbSNP:753697044
610 610 c, t dbSNP:374107920
628 628 c, t dbSNP:766444250
629 629 a, g dbSNP:757373635
634 634 a, c, g, t dbSNP:575169424
635 635 a, g dbSNP:775821469
652 652 c, t dbSNP:765492925
673 673 a, g dbSNP:759963297
676 676 a, g dbSNP:777080257
700 700 g, t dbSNP:771428804
706 706 c, t dbSNP:556632744
707 707 a, g dbSNP:772698843
719 719 g, t dbSNP:771641172
728 728 c, t dbSNP:747728810
729 729 a, g dbSNP:778676398
731 731 c, t dbSNP:730880028
732 732 a, g dbSNP:371074506
736 736 c, t dbSNP:749141733
737 737 a, c dbSNP:80231183
742 742 c, t dbSNP:141146222
745 745 c, t dbSNP:756105407
748 748 a, g dbSNP:750452564
750 750 a, g dbSNP:199826343
757 757 a, c dbSNP:758550918
766 766 c, t dbSNP:752808482
807 807 c, t dbSNP:765525084
812 812 c, t dbSNP:370939488
815 815 c, t dbSNP:199881197
816 816 a, g dbSNP:766789413
817 817 c, t dbSNP:190009503
823 823 c, t dbSNP:773752934
835 835 c, t dbSNP:567800140
866 866 a, g dbSNP:747715758
869 869 c, t dbSNP:147946711
877 877 g, t dbSNP:768360652
878 878 c, t dbSNP:749005381
879 879 a, g dbSNP:141069951
888 888 c, t dbSNP:756014119
889 889 c, t dbSNP:745777122
903 903 a, g dbSNP:781297152
909 909 c, t dbSNP:758365970
910 910 a, c dbSNP:752825007
914 914 a, g dbSNP:377319362
928 928 a, c, g dbSNP:754128746
930 930 c, t dbSNP:766703407
937 937 c, t dbSNP:149475041
949 949 a, g dbSNP:750882247
951 951 g, t dbSNP:768101021
953 953 g, t dbSNP:548026518
961 961 c, t dbSNP:773926584
979 979 c, g dbSNP:768116035
983 983 a, g dbSNP:762662180
988 988 c, t dbSNP:775330235
991 991 a, g dbSNP:769660058
997 997 a, g dbSNP:141922502
1010 1010 c, t dbSNP:780921701
1011 1011 a, g dbSNP:770910730
1015 1015 c, t dbSNP:746979509
1032 1032 a, g dbSNP:779048233
1046 1046 c, t dbSNP:730880025
1047 1047 a, g dbSNP:730880026
1062 1062 c, g dbSNP:754040899
1075 1075 c, t dbSNP:372891488
1076 1076 a, g dbSNP:369027314
1087 1087 c, t dbSNP:147351423
1088 1088 a, g dbSNP:750792560
1089 1089 c, t dbSNP:767856035
1098 1098 c, g dbSNP:762376608
1103 1103 c, t dbSNP:752044060
1113 1113 a, g dbSNP:763637343
1115 1115 a, t dbSNP:762574079
1146 1146 c, t dbSNP:775239569
1153 1153 a, c dbSNP:759049795
1163 1163 c, t dbSNP:769571931
1172 1172 a, g dbSNP:759343956
1177 1177 a, g dbSNP:776470776
1182 1182 a, g dbSNP:770701335
1201 1201 a, g dbSNP:746937350
1205 1205 c, t dbSNP:551859540
1206 1206 a, g dbSNP:768677078
1210 1210 a, c, t dbSNP:531647997
1211 1211 a, g dbSNP:377431750
1228 1228 c, t dbSNP:746135853
1230 1230 c, t dbSNP:781587642
1231 1231 a, g dbSNP:757609748
1234 1234 c, t dbSNP:373071036
1235 1235 a, g dbSNP:368743618
1240 1240 c, t dbSNP:757911669
1243 1243 c, t dbSNP:752266046
1244 1244 a, g dbSNP:764895997
1273 1273 c, t dbSNP:562895831
1279 1279 g, t dbSNP:549035311
1281 1281 c, t dbSNP:766177312
1296 1296 c, t dbSNP:760414026
1297 1297 a, g dbSNP:750069877
1302 1302 g, t dbSNP:773161555
1308 1308 a, g dbSNP:730880027
1324 1324 a, c dbSNP:772002925
1333 1333 g, t dbSNP:749310004
1345 1345 c, t dbSNP:199590938
1346 1346 g, t dbSNP:769899863
1351 1351 a, c dbSNP:746080269
1368 1368 c, g dbSNP:781292239
1371 1371 c, t dbSNP:757601862
1372 1372 g, t dbSNP:14760
1374 1374 a, c, g dbSNP:778363691
1375 1375 a, c, g dbSNP:201092237
1380 1380 a, g dbSNP:753320398
1381 1381 a, c, t dbSNP:754497882
1397 1397 a, t dbSNP:753485163
1399 1399 c, g, t dbSNP:760451884
1407 1407 a, g dbSNP:376008810
1412 1412 a, g dbSNP:772897588
1417 1417 a, g dbSNP:1063394
1426 1426 a, c dbSNP:767359528
1443 1443 a, g dbSNP:142720001
1450 1450 a, g dbSNP:774319060
1458 1458 a, g dbSNP:769814111
1462 1462 c, t dbSNP:369629125
1463 1463 a, g dbSNP:776842527
1467 1467 a, g dbSNP:771067583
1469 1469 -, t dbSNP:765926994
1469 1469 c, t dbSNP:747310177
1470 1470 g, t dbSNP:778275983
1478 1478 c, t dbSNP:772607875
1482 1482 a, c, t dbSNP:376317489
1483 1483 a, g dbSNP:11556349
1541 1541 a, g dbSNP:532913310
1594 1594 c, t dbSNP:9808425
1618 1618 c, t dbSNP:369044508
1635 1635 g, t dbSNP:192126963
1661 1661 c, t dbSNP:188037939
1679 1679 a, g dbSNP:371557574
1690 1690 a, g dbSNP:74429631
1738 1738 a, g dbSNP:541429892
1749 1749 a, t dbSNP:375486393
1751 1751 a, c dbSNP:183276392
1758 1758 a, c dbSNP:760053346
1766 1766 c, t dbSNP:376690665
1807 1807 a, c, t dbSNP:191481780
1829 1829 a, c dbSNP:1054431
1830 1830 a, g dbSNP:187700226
1856 1856 a, g dbSNP:534937714
1879 1879 c, t dbSNP:138911012
1880 1880 a, g dbSNP:539684656
1896 1896 a, g dbSNP:558329660
1900 1900 a, g dbSNP:146214371
1946 1946 g, t dbSNP:569191103
1979 1979 c, t dbSNP:749591134
1981 1981 a, g dbSNP:183003213
2000 2000 -, tct dbSNP:571856046
2006 2006 -, g dbSNP:774698445
2076 2076 c, t dbSNP:778077752

Target ORF information:

RefSeq Version NM_006000
Organism Homo sapiens (human)
Definition Homo sapiens tubulin, alpha 4a (TUBA4A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006000

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGAAT GCATCTCAGT CCACGTGGGG CAGGCAGGTG TCCAGATGGG CAATGCCTGC 
TGGGAGCTCT ATTGCTTGGA ACATGGGATT CAGCCTGATG GGCAGATGCC CAGTGACAAG
ACCATTGGTG GAGGGGACGA CTCCTTCACC ACCTTCTTCT GTGAAACTGG TGCTGGAAAA
CACGTACCCC GGGCAGTTTT TGTGGATCTG GAGCCTACGG TCATTGATGA GATCCGAAAT
GGCCCATACC GACAGCTCTT CCACCCAGAG CAGCTCATCA CTGGGAAAGA GGATGCTGCC
AACAACTATG CCCGTGGTCA CTATACCATT GGCAAGGAGA TCATTGACCC AGTGCTGGAT
CGGATCCGCA AGCTGTCTGA CCAGTGCACA GGACTTCAGG GCTTCCTGGT GTTCCACAGC
TTTGGTGGGG GCACTGGCTC TGGCTTCACC TCACTCCTGA TGGAGCGGCT CTCTGTTGAC
TATGGCAAGA AATCCAAGCT GGAATTCTCC ATCTACCCAG CCCCCCAGGT GTCTACAGCC
GTGGTCGAGC CCTACAACTC TATCCTGACC ACCCACACCA CCCTGGAGCA CTCAGACTGT
GCCTTCATGG TGGACAACGA AGCAATCTAT GACATCTGCC GCCGCAACCT AGACATCGAG
CGCCCAACCT ACACCAACCT CAATCGCCTC ATTAGCCAAA TTGTCTCCTC CATCACAGCT
TCTCTGCGCT TTGACGGGGC CCTCAATGTG GACCTGACAG AGTTCCAGAC CAACCTGGTG
CCCTACCCTC GCATCCACTT CCCCCTGGCC ACCTATGCAC CAGTCATCTC TGCAGAAAAG
GCATACCACG AGCAGCTGTC GGTGGCAGAG ATCACCAATG CCTGCTTTGA GCCTGCCAAC
CAGATGGTAA AGTGTGATCC CCGGCACGGC AAGTACATGG CCTGCTGCCT GCTGTACCGT
GGAGATGTGG TGCCCAAGGA TGTCAACGCT GCCATTGCCG CCATCAAGAC CAAGCGCAGC
ATTCAGTTTG TGGACTGGTG CCCCACAGGC TTCAAGGTTG GTATCAACTA CCAGCCTCCC
ACTGTGGTGC CTGGGGGTGA CCTGGCCAAG GTGCAGCGTG CCGTGTGCAT GCTGAGCAAC
ACGACCGCCA TCGCCGAGGC CTGGGCCCGC CTGGACCACA AGTTCGACCT GATGTATGCC
AAGAGGGCGT TTGTGCACTG GTATGTGGGT GAGGGCATGG AGGAGGGTGA GTTCTCCGAG
GCCCGTGAGG ATATGGCTGC CCTGGAGAAG GATTATGAGG AGGTGGGCAT CGACTCCTAT
GAGGACGAGG ATGAGGGAGA AGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09800
Accession Version NM_001278552.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1302bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tubulin alpha-4A chain isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK299958.1 and AK054731.1. Summary: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]. Transcript Variant: This variant (2) contains an alternate 5' exon, which results in translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299958.1, BE741651.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA2144333 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGCAATG CCTGCTGGGA GCTCTATTGC TTGGAACATG GGATTCAGCC TGATGGGCAG 
ATGCCCAGTG ACAAGACCAT TGGTGGAGGG GACGACTCCT TCACCACCTT CTTCTGTGAA
ACTGGTGCTG GAAAACACGT ACCCCGGGCA GTTTTTGTGG ATCTGGAGCC TACGGTCATT
GATGAGATCC GAAATGGCCC ATACCGACAG CTCTTCCACC CAGAGCAGCT CATCACTGGG
AAAGAGGATG CTGCCAACAA CTATGCCCGT GGTCACTATA CCATTGGCAA GGAGATCATT
GACCCAGTGC TGGATCGGAT CCGCAAGCTG TCTGACCAGT GCACAGGACT TCAGGGCTTC
CTGGTGTTCC ACAGCTTTGG TGGGGGCACT GGCTCTGGCT TCACCTCACT CCTGATGGAG
CGGCTCTCTG TTGACTATGG CAAGAAATCC AAGCTGGAAT TCTCCATCTA CCCAGCCCCC
CAGGTGTCTA CAGCCGTGGT CGAGCCCTAC AACTCTATCC TGACCACCCA CACCACCCTG
GAGCACTCAG ACTGTGCCTT CATGGTGGAC AACGAAGCAA TCTATGACAT CTGCCGCCGC
AACCTAGACA TCGAGCGCCC AACCTACACC AACCTCAATC GCCTCATTAG CCAAATTGTC
TCCTCCATCA CAGCTTCTCT GCGCTTTGAC GGGGCCCTCA ATGTGGACCT GACAGAGTTC
CAGACCAACC TGGTGCCCTA CCCTCGCATC CACTTCCCCC TGGCCACCTA TGCACCAGTC
ATCTCTGCAG AAAAGGCATA CCACGAGCAG CTGTCGGTGG CAGAGATCAC CAATGCCTGC
TTTGAGCCTG CCAACCAGAT GGTAAAGTGT GATCCCCGGC ACGGCAAGTA CATGGCCTGC
TGCCTGCTGT ACCGTGGAGA TGTGGTGCCC AAGGATGTCA ACGCTGCCAT TGCCGCCATC
AAGACCAAGC GCAGCATTCA GTTTGTGGAC TGGTGCCCCA CAGGCTTCAA GGTTGGTATC
AACTACCAGC CTCCCACTGT GGTGCCTGGG GGTGACCTGG CCAAGGTGCA GCGTGCCGTG
TGCATGCTGA GCAACACGAC CGCCATCGCC GAGGCCTGGG CCCGCCTGGA CCACAAGTTC
GACCTGATGT ATGCCAAGAG GGCGTTTGTG CACTGGTATG TGGGTGAGGG CATGGAGGAG
GGTGAGTTCT CCGAGGCCCG TGAGGATATG GCTGCCCTGG AGAAGGATTA TGAGGAGGTG
GGCATCGACT CCTATGAGGA CGAGGATGAG GGAGAAGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001265481.1
CDS557..1858
Misc Feature(1)101..103(+)
Misc Feature(2)557..1855(+)
Misc Feature(3)557..1816(+)
Misc Feature(4)722..1195(+)
Misc Feature(5)725..1183(+)
Misc Feature(6)1106..1567(+)
Exon (1)1..514
Gene:TUBA4A
Gene Synonym:
Exon (2)515..737
Gene:TUBA4A
Gene Synonym:
Exon (3)738..886
Gene:TUBA4A
Gene Synonym:
Exon (4)887..2499
Gene:TUBA4A
Gene Synonym:
Translation
Position Chain Variation Link
23 23 a, g dbSNP:750424321
51 51 c, g dbSNP:559500391
98 98 a, g dbSNP:764908922
115 115 g, t dbSNP:545928182
172 172 a, g dbSNP:45627932
224 224 c, t dbSNP:45496400
226 226 -, c dbSNP:769602490
263 263 c, t dbSNP:45444896
274 274 c, g dbSNP:576941701
301 301 c, g dbSNP:557007249
351 351 a, g dbSNP:45476194
356 356 a, c dbSNP:45476301
399 399 c, g dbSNP:374651868
490 490 a, g dbSNP:534607715
541 541 a, g dbSNP:781322731
553 553 c, t dbSNP:138241750
560 560 a, g dbSNP:751783740
568 568 c, t dbSNP:777029118
577 577 a, g dbSNP:141429751
583 583 c, t dbSNP:537411926
586 586 c, g dbSNP:753230693
595 595 c, t dbSNP:145576984
598 598 a, g dbSNP:373338390
616 616 c, g dbSNP:368969801
620 620 c, t dbSNP:766075261
624 624 c, g dbSNP:760363903
633 633 a, c dbSNP:772807683
634 634 c, t dbSNP:535391274
636 636 c, t dbSNP:201865832
639 639 g, t dbSNP:774232761
641 641 a, g dbSNP:768583053
645 645 c, g dbSNP:745889662
646 646 g, t dbSNP:11556348
649 649 c, t dbSNP:140319726
650 650 a, g, t dbSNP:757156202
680 680 c, g dbSNP:747219812
685 685 c, t dbSNP:777902454
690 690 a, g dbSNP:758743744
693 693 a, g dbSNP:748558025
694 694 c, t dbSNP:376398028
697 697 a, g dbSNP:755558869
714 714 c, t dbSNP:372756767
718 718 a, t dbSNP:765910022
722 722 a, g dbSNP:755620655
730 730 a, g dbSNP:567717225
746 746 c, t dbSNP:780475810
755 755 c, t dbSNP:755609946
760 760 a, c dbSNP:749906171
761 761 c, t dbSNP:757590679
762 762 a, g dbSNP:756888946
765 765 a, g dbSNP:751277119
766 766 a, g dbSNP:763842483
769 769 c, g dbSNP:755752894
781 781 a, g dbSNP:45571337
814 814 c, t dbSNP:752628707
823 823 a, c, t dbSNP:11556347
825 825 a, g dbSNP:760745277
835 835 c, t dbSNP:199579096
839 839 a, g dbSNP:200651096
840 840 c, t dbSNP:201172087
843 843 a, g dbSNP:201649283
848 848 -, gag dbSNP:773787054
853 853 c, t dbSNP:531687027
860 860 c, t dbSNP:762123008
862 862 a, g dbSNP:774757044
872 872 c, t dbSNP:769120095
878 878 c, t dbSNP:749694106
879 879 a, g dbSNP:780543647
880 880 c, t dbSNP:562650415
883 883 a, g dbSNP:746442903
892 892 c, g dbSNP:775703836
910 910 a, g dbSNP:770260351
919 919 a, g dbSNP:372079932
920 920 c, g dbSNP:781745194
938 938 c, g dbSNP:770376188
943 943 a, c dbSNP:746551411
944 944 a, c dbSNP:730880029
952 952 c, t dbSNP:777343840
976 976 a, g dbSNP:368461384
978 978 a, g dbSNP:752457075
993 993 a, g dbSNP:778571547
994 994 c, t dbSNP:754843931
1014 1014 a, g dbSNP:753697044
1033 1033 c, t dbSNP:374107920
1051 1051 c, t dbSNP:766444250
1052 1052 a, g dbSNP:757373635
1057 1057 a, c, g, t dbSNP:575169424
1058 1058 a, g dbSNP:775821469
1075 1075 c, t dbSNP:765492925
1096 1096 a, g dbSNP:759963297
1099 1099 a, g dbSNP:777080257
1123 1123 g, t dbSNP:771428804
1129 1129 c, t dbSNP:556632744
1130 1130 a, g dbSNP:772698843
1142 1142 g, t dbSNP:771641172
1151 1151 c, t dbSNP:747728810
1152 1152 a, g dbSNP:778676398
1154 1154 c, t dbSNP:730880028
1155 1155 a, g dbSNP:371074506
1159 1159 c, t dbSNP:749141733
1160 1160 a, c dbSNP:80231183
1165 1165 c, t dbSNP:141146222
1168 1168 c, t dbSNP:756105407
1171 1171 a, g dbSNP:750452564
1173 1173 a, g dbSNP:199826343
1180 1180 a, c dbSNP:758550918
1189 1189 c, t dbSNP:752808482
1230 1230 c, t dbSNP:765525084
1235 1235 c, t dbSNP:370939488
1238 1238 c, t dbSNP:199881197
1239 1239 a, g dbSNP:766789413
1240 1240 c, t dbSNP:190009503
1246 1246 c, t dbSNP:773752934
1258 1258 c, t dbSNP:567800140
1289 1289 a, g dbSNP:747715758
1292 1292 c, t dbSNP:147946711
1300 1300 g, t dbSNP:768360652
1301 1301 c, t dbSNP:749005381
1302 1302 a, g dbSNP:141069951
1311 1311 c, t dbSNP:756014119
1312 1312 c, t dbSNP:745777122
1326 1326 a, g dbSNP:781297152
1332 1332 c, t dbSNP:758365970
1333 1333 a, c dbSNP:752825007
1337 1337 a, g dbSNP:377319362
1351 1351 a, c, g dbSNP:754128746
1353 1353 c, t dbSNP:766703407
1360 1360 c, t dbSNP:149475041
1372 1372 a, g dbSNP:750882247
1374 1374 g, t dbSNP:768101021
1376 1376 g, t dbSNP:548026518
1384 1384 c, t dbSNP:773926584
1402 1402 c, g dbSNP:768116035
1406 1406 a, g dbSNP:762662180
1411 1411 c, t dbSNP:775330235
1414 1414 a, g dbSNP:769660058
1420 1420 a, g dbSNP:141922502
1433 1433 c, t dbSNP:780921701
1434 1434 a, g dbSNP:770910730
1438 1438 c, t dbSNP:746979509
1455 1455 a, g dbSNP:779048233
1469 1469 c, t dbSNP:730880025
1470 1470 a, g dbSNP:730880026
1485 1485 c, g dbSNP:754040899
1498 1498 c, t dbSNP:372891488
1499 1499 a, g dbSNP:369027314
1510 1510 c, t dbSNP:147351423
1511 1511 a, g dbSNP:750792560
1512 1512 c, t dbSNP:767856035
1521 1521 c, g dbSNP:762376608
1526 1526 c, t dbSNP:752044060
1536 1536 a, g dbSNP:763637343
1538 1538 a, t dbSNP:762574079
1569 1569 c, t dbSNP:775239569
1576 1576 a, c dbSNP:759049795
1586 1586 c, t dbSNP:769571931
1595 1595 a, g dbSNP:759343956
1600 1600 a, g dbSNP:776470776
1605 1605 a, g dbSNP:770701335
1624 1624 a, g dbSNP:746937350
1628 1628 c, t dbSNP:551859540
1629 1629 a, g dbSNP:768677078
1633 1633 a, c, t dbSNP:531647997
1634 1634 a, g dbSNP:377431750
1651 1651 c, t dbSNP:746135853
1653 1653 c, t dbSNP:781587642
1654 1654 a, g dbSNP:757609748
1657 1657 c, t dbSNP:373071036
1658 1658 a, g dbSNP:368743618
1663 1663 c, t dbSNP:757911669
1666 1666 c, t dbSNP:752266046
1667 1667 a, g dbSNP:764895997
1696 1696 c, t dbSNP:562895831
1702 1702 g, t dbSNP:549035311
1704 1704 c, t dbSNP:766177312
1719 1719 c, t dbSNP:760414026
1720 1720 a, g dbSNP:750069877
1725 1725 g, t dbSNP:773161555
1731 1731 a, g dbSNP:730880027
1747 1747 a, c dbSNP:772002925
1756 1756 g, t dbSNP:749310004
1768 1768 c, t dbSNP:199590938
1769 1769 g, t dbSNP:769899863
1774 1774 a, c dbSNP:746080269
1791 1791 c, g dbSNP:781292239
1794 1794 c, t dbSNP:757601862
1795 1795 g, t dbSNP:14760
1797 1797 a, c, g dbSNP:778363691
1798 1798 a, c, g dbSNP:201092237
1803 1803 a, g dbSNP:753320398
1804 1804 a, c, t dbSNP:754497882
1820 1820 a, t dbSNP:753485163
1822 1822 c, g, t dbSNP:760451884
1830 1830 a, g dbSNP:376008810
1835 1835 a, g dbSNP:772897588
1840 1840 a, g dbSNP:1063394
1849 1849 a, c dbSNP:767359528
1866 1866 a, g dbSNP:142720001
1873 1873 a, g dbSNP:774319060
1881 1881 a, g dbSNP:769814111
1885 1885 c, t dbSNP:369629125
1886 1886 a, g dbSNP:776842527
1890 1890 a, g dbSNP:771067583
1892 1892 -, t dbSNP:765926994
1892 1892 c, t dbSNP:747310177
1893 1893 g, t dbSNP:778275983
1901 1901 c, t dbSNP:772607875
1905 1905 a, c, t dbSNP:376317489
1906 1906 a, g dbSNP:11556349
1964 1964 a, g dbSNP:532913310
2017 2017 c, t dbSNP:9808425
2041 2041 c, t dbSNP:369044508
2058 2058 g, t dbSNP:192126963
2084 2084 c, t dbSNP:188037939
2102 2102 a, g dbSNP:371557574
2113 2113 a, g dbSNP:74429631
2161 2161 a, g dbSNP:541429892
2172 2172 a, t dbSNP:375486393
2174 2174 a, c dbSNP:183276392
2181 2181 a, c dbSNP:760053346
2189 2189 c, t dbSNP:376690665
2230 2230 a, c, t dbSNP:191481780
2252 2252 a, c dbSNP:1054431
2253 2253 a, g dbSNP:187700226
2279 2279 a, g dbSNP:534937714
2302 2302 c, t dbSNP:138911012
2303 2303 a, g dbSNP:539684656
2319 2319 a, g dbSNP:558329660
2323 2323 a, g dbSNP:146214371
2369 2369 g, t dbSNP:569191103
2402 2402 c, t dbSNP:749591134
2404 2404 a, g dbSNP:183003213
2423 2423 -, tct dbSNP:571856046
2429 2429 -, g dbSNP:774698445
2499 2499 c, t dbSNP:778077752

Target ORF information:

RefSeq Version NM_001278552
Organism Homo sapiens (human)
Definition Homo sapiens tubulin, alpha 4a (TUBA4A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001278552

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGCAATG CCTGCTGGGA GCTCTATTGC TTGGAACATG GGATTCAGCC TGATGGGCAG 
ATGCCCAGTG ACAAGACCAT TGGTGGAGGG GACGACTCCT TCACCACCTT CTTCTGTGAA
ACTGGTGCTG GAAAACACGT ACCCCGGGCA GTTTTTGTGG ATCTGGAGCC TACGGTCATT
GATGAGATCC GAAATGGCCC ATACCGACAG CTCTTCCACC CAGAGCAGCT CATCACTGGG
AAAGAGGATG CTGCCAACAA CTATGCCCGT GGTCACTATA CCATTGGCAA GGAGATCATT
GACCCAGTGC TGGATCGGAT CCGCAAGCTG TCTGACCAGT GCACAGGACT TCAGGGCTTC
CTGGTGTTCC ACAGCTTTGG TGGGGGCACT GGCTCTGGCT TCACCTCACT CCTGATGGAG
CGGCTCTCTG TTGACTATGG CAAGAAATCC AAGCTGGAAT TCTCCATCTA CCCAGCCCCC
CAGGTGTCTA CAGCCGTGGT CGAGCCCTAC AACTCTATCC TGACCACCCA CACCACCCTG
GAGCACTCAG ACTGTGCCTT CATGGTGGAC AACGAAGCAA TCTATGACAT CTGCCGCCGC
AACCTAGACA TCGAGCGCCC AACCTACACC AACCTCAATC GCCTCATTAG CCAAATTGTC
TCCTCCATCA CAGCTTCTCT GCGCTTTGAC GGGGCCCTCA ATGTGGACCT GACAGAGTTC
CAGACCAACC TGGTGCCCTA CCCTCGCATC CACTTCCCCC TGGCCACCTA TGCACCAGTC
ATCTCTGCAG AAAAGGCATA CCACGAGCAG CTGTCGGTGG CAGAGATCAC CAATGCCTGC
TTTGAGCCTG CCAACCAGAT GGTAAAGTGT GATCCCCGGC ACGGCAAGTA CATGGCCTGC
TGCCTGCTGT ACCGTGGAGA TGTGGTGCCC AAGGATGTCA ACGCTGCCAT TGCCGCCATC
AAGACCAAGC GCAGCATTCA GTTTGTGGAC TGGTGCCCCA CAGGCTTCAA GGTTGGTATC
AACTACCAGC CTCCCACTGT GGTGCCTGGG GGTGACCTGG CCAAGGTGCA GCGTGCCGTG
TGCATGCTGA GCAACACGAC CGCCATCGCC GAGGCCTGGG CCCGCCTGGA CCACAAGTTC
GACCTGATGT ATGCCAAGAG GGCGTTTGTG CACTGGTATG TGGGTGAGGG CATGGAGGAG
GGTGAGTTCT CCGAGGCCCG TGAGGATATG GCTGCCCTGG AGAAGGATTA TGAGGAGGTG
GGCATCGACT CCTATGAGGA CGAGGATGAG GGAGAAGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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