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MAGED4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAGED4
Entrez Gene ID 728239
Full Name melanoma antigen family D4
Synonyms MAGE-E1, MAGE1, MAGEE1
General protein information
Preferred Names
melanoma-associated antigen D4
Names
melanoma-associated antigen D4
MAGE-D4 antigen
MAGE-E1 antigen
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp11.22

Summary lac of sum
Disorder MIM:

300702

Disorder Html:





The following MAGED4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGED4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu05151 NM_001272061 Homo sapiens melanoma antigen family D4 (MAGED4), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$391.30
$559.00
OHu05612 NM_001272063 Homo sapiens melanoma antigen family D4 (MAGED4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$391.30
$559.00
OHu05177 NM_001098800 Homo sapiens melanoma antigen family D4 (MAGED4), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD Quote Price
OHu05612 NM_001272062 Homo sapiens melanoma antigen family D4 (MAGED4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$391.30
$559.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu05151
Accession Version NM_001272061.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2274bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen D4 isoform 3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC245177.1, AK289458.1 and BC063838.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (3). This transcript structure is inferred from aligning transcripts. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GAACCCGGAG
CTCAGAGAGG AGACGCCTCT GAGCATGGCT CCCAGCTGGT ACCTGGAATA CAAGAAGATC
CCCAACAGCA ACCCACCTGA GTATGAATTC CTCTGGGGCC TGCGAGCCCG CCATGAGACC
AGCAAGATGA GGGTCCTGAG ATTCATCGCC CAGAATCAGA ACCGAGACCC CCGGGAATGG
AAGGCTCATT TCTTGGAGGC TGTGGATGAT GCTTTCAAGA CAATGGATGT GGATATGGCC
GAGGAACATG CCAGGGCCCA GATGAGGGCC CAGATGAATA TCGGGGATGA AGCGCTGATT
GGACGGTGGA GCTGGGATGA CATACAAGTC GAGCTCCTGA CCTGGGATGA GGACGGAGAT
TTTGGCGATG CCTGGGCCAG GATCCCCTTT GCTTTCTGGG CCAGATACCA TCAGTACATT
CTGAATAGCA ACCGTGCCAA CAGGAGGGCC ACGTGGAGAG CTGGCGTCAG CAGTGGCACC
AATGGAGGGG CCAGCACCAG CGTCCTAGAT GGCCCCAGCA CCAGCTCCAC CATCCGGACC
AGAAATGCTG CCAGAGCTGG CGCCAGCTTC TTCTCCTGGA TCCAGCACCG TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001258990.1
CDS196..2469
Misc Feature(1)112..114(+)
Misc Feature(2)1453..2010(+)
Exon (1)1..143
Gene:MAGED4
Gene Synonym:
Exon (2)144..405
Gene:MAGED4
Gene Synonym:
Exon (3)406..1239
Gene:MAGED4
Gene Synonym:
Exon (4)1240..1443
Gene:MAGED4
Gene Synonym:
Exon (5)1444..1507
Gene:MAGED4
Gene Synonym:
Exon (6)1508..1587
Gene:MAGED4
Gene Synonym:
Exon (7)1588..1679
Gene:MAGED4
Gene Synonym:
Exon (8)1680..1759
Gene:MAGED4
Gene Synonym:
Exon (9)1760..1802
Gene:MAGED4
Gene Synonym:
Exon (10)1803..1865
Gene:MAGED4
Gene Synonym:
Exon (11)1866..1913
Gene:MAGED4
Gene Synonym:
Exon (12)1914..2028
Gene:MAGED4
Gene Synonym:
Exon (13)2029..2459
Gene:MAGED4
Gene Synonym:
Exon (14)2460..2671
Gene:MAGED4
Gene Synonym:
Translation
Position Chain Variation Link
390 390 c, t dbSNP:201653231
2340 2340 c, g, t dbSNP:782203103
2341 2341 a, g dbSNP:782042156
2368 2368 a, g dbSNP:782149392
2377 2377 a, g dbSNP:782394547
2411 2411 a, g dbSNP:781992579
2426 2426 c, g dbSNP:782089484
2438 2438 c, g dbSNP:782724575
2440 2440 a, g dbSNP:781932311
2465 2465 a, g dbSNP:782779782
2480 2480 c, t dbSNP:781986192
2481 2481 a, g dbSNP:368363830

Target ORF information:

RefSeq Version NM_001272061
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family D4 (MAGED4), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001272061

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GAACCCGGAG
CTCAGAGAGG AGACGCCTCT GAGCATGGCT CCCAGCTGGT ACCTGGAATA CAAGAAGATC
CCCAACAGCA ACCCACCTGA GTATGAATTC CTCTGGGGCC TGCGAGCCCG CCATGAGACC
AGCAAGATGA GGGTCCTGAG ATTCATCGCC CAGAATCAGA ACCGAGACCC CCGGGAATGG
AAGGCTCATT TCTTGGAGGC TGTGGATGAT GCTTTCAAGA CAATGGATGT GGATATGGCC
GAGGAACATG CCAGGGCCCA GATGAGGGCC CAGATGAATA TCGGGGATGA AGCGCTGATT
GGACGGTGGA GCTGGGATGA CATACAAGTC GAGCTCCTGA CCTGGGATGA GGACGGAGAT
TTTGGCGATG CCTGGGCCAG GATCCCCTTT GCTTTCTGGG CCAGATACCA TCAGTACATT
CTGAATAGCA ACCGTGCCAA CAGGAGGGCC ACGTGGAGAG CTGGCGTCAG CAGTGGCACC
AATGGAGGGG CCAGCACCAG CGTCCTAGAT GGCCCCAGCA CCAGCTCCAC CATCCGGACC
AGAAATGCTG CCAGAGCTGG CGCCAGCTTC TTCTCCTGGA TCCAGCACCG TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu05612
Accession Version NM_001272063.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen D4 isoform 1
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC245177.1, AK289458.1 and BC063838.1. Transcript Variant: This variant (1) differs in the 5' UTR and lacks an alternate exon in the coding region, compared to variant 4. It encodes isoform 1, which is shorter than isoform 3. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGCAC
CGTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001258992.1
CDS175..2400
Misc Feature(1)112..114(+)
Misc Feature(2)1432..1941(+)
Exon (1)1..143
Gene:MAGED4
Gene Synonym:
Exon (2)144..384
Gene:MAGED4
Gene Synonym:
Exon (3)385..1218
Gene:MAGED4
Gene Synonym:
Exon (4)1219..1422
Gene:MAGED4
Gene Synonym:
Exon (5)1423..1486
Gene:MAGED4
Gene Synonym:
Exon (6)1487..1566
Gene:MAGED4
Gene Synonym:
Exon (7)1567..1658
Gene:MAGED4
Gene Synonym:
Exon (8)1659..1738
Gene:MAGED4
Gene Synonym:
Exon (9)1739..1781
Gene:MAGED4
Gene Synonym:
Exon (10)1782..1844
Gene:MAGED4
Gene Synonym:
Exon (11)1845..1959
Gene:MAGED4
Gene Synonym:
Exon (12)1960..2390
Gene:MAGED4
Gene Synonym:
Exon (13)2391..2602
Gene:MAGED4
Gene Synonym:
Translation
Position Chain Variation Link
369 369 c, t dbSNP:201653231
2271 2271 c, g, t dbSNP:782203103
2272 2272 a, g dbSNP:782042156
2299 2299 a, g dbSNP:782149392
2308 2308 a, g dbSNP:782394547
2342 2342 a, g dbSNP:781992579
2357 2357 c, g dbSNP:782089484
2369 2369 c, g dbSNP:782724575
2371 2371 a, g dbSNP:781932311
2396 2396 a, g dbSNP:782779782
2411 2411 c, t dbSNP:781986192
2412 2412 a, g dbSNP:368363830

Target ORF information:

RefSeq Version NM_001272063
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family D4 (MAGED4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001272063

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGCAC
CGTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu05177
Accession Version NM_001098800.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2220bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen D4 isoform 2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC245177.1, AK289458.1 and BC063838.1. On Jan 5, 2013 this sequence version replaced gi:149274613. Transcript Variant: This variant (3) differs in the 5' UTR, lacks an alternate exon, and uses an alternate splice site in the 3' coding region, compared to variant 4. This results in a frameshift and an early stop codon. The encoded protein (isoform 2) is shorter and has a distinct C-terminus, compared to isoform 3. This transcript structure is inferred from aligning transcripts. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001092270.1
CDS145..2364
Misc Feature(1)112..114(+)
Misc Feature(2)1402..1911(+)
Exon (1)1..143
Gene:MAGED4
Gene Synonym:
Exon (2)144..354
Gene:MAGED4
Gene Synonym:
Exon (3)355..1188
Gene:MAGED4
Gene Synonym:
Exon (4)1189..1392
Gene:MAGED4
Gene Synonym:
Exon (5)1393..1456
Gene:MAGED4
Gene Synonym:
Exon (6)1457..1536
Gene:MAGED4
Gene Synonym:
Exon (7)1537..1628
Gene:MAGED4
Gene Synonym:
Exon (8)1629..1708
Gene:MAGED4
Gene Synonym:
Exon (9)1709..1751
Gene:MAGED4
Gene Synonym:
Exon (10)1752..1814
Gene:MAGED4
Gene Synonym:
Exon (11)1815..1929
Gene:MAGED4
Gene Synonym:
Exon (12)1930..2372
Gene:MAGED4
Gene Synonym:
Exon (13)2373..2584
Gene:MAGED4
Gene Synonym:
Translation
Position Chain Variation Link
339 339 c, t dbSNP:201653231
2241 2241 c, g, t dbSNP:782203103
2242 2242 a, g dbSNP:782042156
2269 2269 a, g dbSNP:782149392
2278 2278 a, g dbSNP:782394547
2312 2312 a, g dbSNP:781992579
2327 2327 c, g dbSNP:782089484
2339 2339 c, g dbSNP:782724575
2341 2341 a, g dbSNP:781932311
2378 2378 a, g dbSNP:782779782
2393 2393 c, t dbSNP:781986192
2394 2394 a, g dbSNP:368363830

Target ORF information:

RefSeq Version NM_001098800
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family D4 (MAGED4), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001098800

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu05612
Accession Version NM_001272062.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen D4 isoform 1
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC245177.1, AK289458.1 and BC063838.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate exon in the coding region, compared to variant 4. It encodes isoform 1, which is shorter than isoform 3. Variants 1 and 2 encode the same isoform (1). This transcript structure is inferred from aligning transcripts. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGCAC
CGTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001258991.1
CDS145..2370
Misc Feature(1)112..114(+)
Misc Feature(2)1402..1911(+)
Exon (1)1..143
Gene:MAGED4
Gene Synonym:
Exon (2)144..354
Gene:MAGED4
Gene Synonym:
Exon (3)355..1188
Gene:MAGED4
Gene Synonym:
Exon (4)1189..1392
Gene:MAGED4
Gene Synonym:
Exon (5)1393..1456
Gene:MAGED4
Gene Synonym:
Exon (6)1457..1536
Gene:MAGED4
Gene Synonym:
Exon (7)1537..1628
Gene:MAGED4
Gene Synonym:
Exon (8)1629..1708
Gene:MAGED4
Gene Synonym:
Exon (9)1709..1751
Gene:MAGED4
Gene Synonym:
Exon (10)1752..1814
Gene:MAGED4
Gene Synonym:
Exon (11)1815..1929
Gene:MAGED4
Gene Synonym:
Exon (12)1930..2360
Gene:MAGED4
Gene Synonym:
Exon (13)2361..2572
Gene:MAGED4
Gene Synonym:
Translation
Position Chain Variation Link
339 339 c, t dbSNP:201653231
2241 2241 c, g, t dbSNP:782203103
2242 2242 a, g dbSNP:782042156
2269 2269 a, g dbSNP:782149392
2278 2278 a, g dbSNP:782394547
2312 2312 a, g dbSNP:781992579
2327 2327 c, g dbSNP:782089484
2339 2339 c, g dbSNP:782724575
2341 2341 a, g dbSNP:781932311
2366 2366 a, g dbSNP:782779782
2381 2381 c, t dbSNP:781986192
2382 2382 a, g dbSNP:368363830

Target ORF information:

RefSeq Version NM_001272062
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family D4 (MAGED4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001272062

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCTGAGG GAAGCTTCAG CGTGCAATCG GAAAGCTACA GTGTTGAAGA CATGGATGAG 
GGTAGCGACG AAGTCGGGGA GGAAGAGATG GTTGAAGGCA ACGACTATGA AGAATTCGGT
GCGTTTGGTG GCTATGGCAC CCTCACCAGC TTTGACATCC ATATCCTCAG AGCCTTCGGA
AGCTTGGGTC CAGGCCTTCG CATCTTATCG AATGAGCCCT GGGAACTGGA AAACCCTGTG
CTGGCCCAGA CCCTGGTGGA GGCATTGCAG CTGGATCCGG AAACACTTGC CAATGAGACG
GCCGCCCGTG CTGCCAACGT AGCCCGCGCC GCCGCCTCCA ACCGTGCGGC TCGGGCCGCT
GCCGCCGCTG CCCGTACCGC CTTCAGTCAG GTGGTCGCTA GCCACCGGGT GGCCACGCCG
CAGGTCTCAG GAGAGGATAC CCAGCCCACG ACCTACGCCG CCGAGGCTCA GGGGCCCACC
CCTGAGCCAC CCCTTGCTTC TCCGCAGACC TCCCAGATGT TAGTCACCAG TAAGATGGCT
GCCCCCGAGG CTCCGGCAAC CTCCGCACAG TCCCAGACAG GCTCCCCGGC CCAGGAGGCT
GCTACTGAGG GCCCTAGTAG CGCCTGTGCT TTCTCTCAGG CTCCGTGTGC CAGGGAGGTG
GACGCCAACC GGCCCAGCAC AGCCTTCCTG GGCCAGAATG ATGTCTTCGA TTTCACTCAG
CCGGCAGGTG TCAGTGGCAT GGCCTTCCCG CGCCCCAAGA GACCTGCCCC AGCCCAAGAG
GCTGCCACAG AGGGCCCCAG TGCTGCCTCT GGTGTGCCCC AGACGGGACC TGGCAGGGAG
GTGGCAGCCA CCCGGCCCAA GACCACCAAG TCGGGGAAGG CGCTGGCCAA GACTCGGTGG
GTGGAGCCTC AGAATGTTGT GGCAGCAGCT GCTGCCAAGG CCAAGATGGC CACGAGCATC
CCTGAGCCGG AGGGTGCAGC TGCTGCCACT GCTCAGCACA GTGCTGAGCC CTGGGCCAGG
ATGGGAGGCA AGAGGACCAA GAAGTCCAAG CACCTGGATG ATGAGTATGA GAGCAGCGAG
GAGGAGAGAG AGACTCCCGC GGTCCCACCC ACCTGGAGAG CATCACAGCC CTCATTGACG
GTGCGGGCTC AGTTGGCCCC TCGGCCCCCG ATGGCCCCGA GGTCCCAGAT ACCCTCAAGG
CACGTACTGT GCCTGCCCCC CCGCAACGTG ACCCTTCTGC AGGAGAGGGC AAATAAGTTG
GTGAAATACC TGATGATTAA GGACTACAAG AAGATCCCCA TCAAGCGCGC AGACATGCTG
AAGGATGTCA TCAGAGAATA TGATGAACAT TTCCCTGAGA TCATTGAACG AGCAACGTAC
ACCCTGGAAA AGAAGTTTGG GATCCACCTG AAGGAGATCG ACAAGGAAGA ACACCTGTAT
ATTCTTGTCT GCACACGGGA CTCCTCAGCT CGCCTCCTTG GAAAAACCAA GGACACTCCC
AGGCTGAGTC TCCTCTTGGT GATTCTGGGC GTCATCTTCA TGAATGGCAA CCGTGCCAGC
GAGGCTGTCC TCTGGGAGGC ACTACGCAAG ATGGGACTGC GCCCTGGGGT GAGGCACCCA
TTCCTCGGCG ATCTGAGGAA GCTCATCACA GATGACTTTG TGAAGCAGAA GTACCTGGAA
TACAAGAAGA TCCCCAACAG CAACCCACCT GAGTATGAAT TCCTCTGGGG CCTGCGAGCC
CGCCATGAGA CCAGCAAGAT GAGGGTCCTG AGATTCATCG CCCAGAATCA GAACCGAGAC
CCCCGGGAAT GGAAGGCTCA TTTCTTGGAG GCTGTGGATG ATGCTTTCAA GACAATGGAT
GTGGATATGG CCGAGGAACA TGCCAGGGCC CAGATGAGGG CCCAGATGAA TATCGGGGAT
GAAGCGCTGA TTGGACGGTG GAGCTGGGAT GACATACAAG TCGAGCTCCT GACCTGGGAT
GAGGACGGAG ATTTTGGCGA TGCCTGGGCC AGGATCCCCT TTGCTTTCTG GGCCAGATAC
CATCAGTACA TTCTGAATAG CAACCGTGCC AACAGGAGGG CCACGTGGAG AGCTGGCGTC
AGCAGTGGCA CCAATGGAGG GGCCAGCACC AGCGTCCTAG ATGGCCCCAG CACCAGCTCC
ACCATCCGGA CCAGAAATGC TGCCAGAGCT GGCGCCAGCT TCTTCTCCTG GATCCAGCAC
CGTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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