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UCHL3 ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) [Homo sapiens (human)]

Gene Symbol UCHL3
Entrez Gene ID 7347
Full Name ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Synonyms UCH-L3
General protein information
Preferred Names
ubiquitin carboxyl-terminal hydrolase isozyme L3
Names
ubiquitin carboxyl-terminal hydrolase isozyme L3
ubiquitin thiolesterase
ubiquitin thioesterase L3
NP_001257881.1
NP_005993.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

13

13q22.2

Summary The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]. lac of sum
Disorder MIM:

603090

Disorder Html:





The following UCHL3 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UCHL3 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu61217 XM_011535212 PREDICTED: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu61218 XM_011535213 PREDICTED: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu61219 XM_011535214 PREDICTED: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu10261 NM_001270952 Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.3
$99
OHu56052 NM_006002 Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.3
$99

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu61217
Accession Version XM_011535212.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 885bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGTCCTCC
TCGCCATCCT CATCACAGCC TCATTCTAGC CACTGCAGAA CGAAGGCCTC ATCATTGTGT
CACCATGCAT CCCTGCCCTG GGTCAAACGT AACCATGTAG GCCCTGCAAA GGCTACCAGT
CCATCTCTCC GTCTTCGTCG CTGGCGACCC TTCTTACGTC TACCATCTTT GGGTCTCCAT
TCTGTTGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGATGGGCGG AAGCCATTTC CAATTAACCA TGGTGAAACT
AGTGATGAAA CTTTATTAGA GGATGCCATA GAAGTTTGCA AGAAGTTTAT GGAGCGCGAC
CCTGATGAAC TAAGATTTAA TGCGATTGCT CTTTCTGCAG CATAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011533514.1
CDS72..956
Misc Feature(1)87..944(+)
Misc Feature(2)93..902(+)
Misc Feature(3)93..770(+)
Misc Feature(4)336..815(+)
Translation
Position Chain Variation Link
17 17 -, ggcggcggcggcgaa dbSNP:149695719
23 23 a, g dbSNP:779137098
26 26 -, ggcgaaggcggcggc dbSNP:8192737
29 29 a, g dbSNP:750111627
31 31 -, ggcggcggcggcgaa dbSNP:769130927
35 35 g, t dbSNP:755762408
37 37 c, g, t dbSNP:765711293
44 44 c, t dbSNP:758831328
45 45 a, t dbSNP:778468100
46 46 a, c, g dbSNP:367636758
49 49 c, t dbSNP:757382777
52 52 g, t dbSNP:781089163
54 54 a, g dbSNP:746104640
57 57 c, t dbSNP:201923490
58 58 a, c, t dbSNP:199605560
62 62 c, t dbSNP:7996485
65 65 c, g dbSNP:528032236
66 66 a, g dbSNP:148862350
70 70 c, t dbSNP:138830150
72 72 a, t dbSNP:772541882
73 73 c, g, t dbSNP:760249794
78 78 a, g dbSNP:753514395
79 79 a, g dbSNP:758887565
81 81 c, g dbSNP:764320440
85 85 a, g dbSNP:535349329
106 106 a, g dbSNP:141970788
110 110 c, g dbSNP:781342676
116 116 c, t dbSNP:569027565
128 128 g, t dbSNP:755293457
148 148 a, t dbSNP:781516708
152 152 c, t dbSNP:779043780
155 155 c, t dbSNP:752943510
156 156 c, t dbSNP:758153494
164 164 c, t dbSNP:192624215
165 165 a, g dbSNP:746951082
170 170 c, t dbSNP:752958414
172 172 c, t dbSNP:781258928
182 182 a, g dbSNP:745434848
188 188 c, t dbSNP:756325539
191 191 a, g dbSNP:111653662
195 195 c, t dbSNP:775053154
201 201 a, g dbSNP:372889994
206 206 a, g dbSNP:772354880
208 208 c, g dbSNP:773497877
221 221 c, t dbSNP:761015496
229 229 g, t dbSNP:146293310
260 260 a, c dbSNP:764474703
266 266 -, cagaa dbSNP:760217672
273 273 a, g dbSNP:553927686
287 287 a, t dbSNP:376728987
296 296 c, t dbSNP:767370396
301 301 g, t dbSNP:141870275
302 302 a, g dbSNP:756013143
309 309 a, g dbSNP:779443132
313 313 c, t dbSNP:753456567
316 316 c, t dbSNP:547414245
345 345 a, g dbSNP:754453843
349 349 a, g dbSNP:778587420
355 355 a, g dbSNP:747751143
364 364 c, t dbSNP:771320095
375 375 c, g dbSNP:781555331
395 395 a, g dbSNP:376626341
405 405 c, t dbSNP:572185099
416 416 c, t dbSNP:764743569
418 418 c, g dbSNP:200965579
420 420 c, t dbSNP:757903004
423 423 a, c, g dbSNP:12872971
433 433 a, g dbSNP:751145532
437 437 c, g dbSNP:756520815
444 444 a, g dbSNP:527890812
450 450 a, g, t dbSNP:749816740
452 452 a, g dbSNP:779548682
460 460 a, g dbSNP:748304370
471 471 c, t dbSNP:374305257
472 472 a, g dbSNP:11555620
485 485 a, g dbSNP:377416621
494 494 c, t dbSNP:747161304
500 500 c, t dbSNP:773674690
503 503 c, g dbSNP:761371610
504 504 c, t dbSNP:767343926
505 505 a, g dbSNP:750068505
514 514 a, g dbSNP:760002431
515 515 c, t dbSNP:765657943
546 546 -, tcc dbSNP:35701234
550 550 -, cct dbSNP:8192754
551 551 -, ctc dbSNP:68176896
551 551 c, g dbSNP:74094453
552 552 -, tcg dbSNP:199782321
553 553 c, t dbSNP:771659221
577 577 c, t dbSNP:556746768
592 592 c, t dbSNP:773031541
622 622 a, c dbSNP:578223221
628 628 c, t dbSNP:545089974
648 648 c, g dbSNP:765712894
675 675 c, t dbSNP:753063819
681 681 c, t dbSNP:763374002
687 687 c, t dbSNP:369614682
691 691 g, t dbSNP:764553436
697 697 a, g dbSNP:752087544
704 704 c, t dbSNP:553853730
708 708 c, g, t dbSNP:780661979
711 711 c, g dbSNP:757346726
741 741 c, t dbSNP:375314560
742 742 c, t dbSNP:772792014
745 745 a, g dbSNP:201962508
747 747 a, g dbSNP:770776286
749 749 a, g dbSNP:776318628
753 753 a, g dbSNP:200687621
757 757 a, g dbSNP:539792961
763 763 a, g dbSNP:764475282
767 767 a, t dbSNP:188855227
770 770 c, t dbSNP:371408920
782 782 a, g dbSNP:767594664
789 789 a, g dbSNP:750557779
797 797 a, g dbSNP:756180319
803 803 a, c dbSNP:770002848
814 814 a, g dbSNP:770547010
819 819 c, t dbSNP:776173957
833 833 -, a dbSNP:777059224
841 841 a, g dbSNP:745490042
854 854 c, t dbSNP:768902862
859 859 a, c dbSNP:774717261
861 861 a, g dbSNP:762150228
891 891 a, c, g dbSNP:752414958
892 892 a, g dbSNP:150677527
893 893 a, g dbSNP:147520161
902 902 -, g dbSNP:766467942
903 903 a, g dbSNP:772405094
904 904 a, c dbSNP:773730866
905 905 a, g dbSNP:761103437
906 906 c, t dbSNP:770928527
907 907 a, g dbSNP:776829672
908 908 c, t dbSNP:561552308
909 909 a, g dbSNP:765533437
914 914 c, t dbSNP:376497370
917 917 g, t dbSNP:760310101
934 934 c, t dbSNP:139878255
935 935 a, g dbSNP:763937455
942 942 c, g dbSNP:751552546
952 952 c, t dbSNP:757164158
953 953 a, g dbSNP:780980995
966 966 a, t dbSNP:750019599
967 967 a, g, t dbSNP:755549913
970 970 a, g dbSNP:748891906
988 988 c, t dbSNP:772458113
992 992 a, t dbSNP:371424841
1007 1007 c, g dbSNP:778053733
1017 1017 c, g dbSNP:532076425
1029 1029 a, c dbSNP:762029977
1044 1044 a, g dbSNP:550058570
1050 1050 g, t dbSNP:2274048

Target ORF information:

RefSeq Version XM_011535212
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535212

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGTCCTCC
TCGCCATCCT CATCACAGCC TCATTCTAGC CACTGCAGAA CGAAGGCCTC ATCATTGTGT
CACCATGCAT CCCTGCCCTG GGTCAAACGT AACCATGTAG GCCCTGCAAA GGCTACCAGT
CCATCTCTCC GTCTTCGTCG CTGGCGACCC TTCTTACGTC TACCATCTTT GGGTCTCCAT
TCTGTTGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGATGGGCGG AAGCCATTTC CAATTAACCA TGGTGAAACT
AGTGATGAAA CTTTATTAGA GGATGCCATA GAAGTTTGCA AGAAGTTTAT GGAGCGCGAC
CCTGATGAAC TAAGATTTAA TGCGATTGCT CTTTCTGCAG CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61218
Accession Version XM_011535213.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 873bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGTTTCTTAA ACAATTAGGT 
CTACATCCTA ACTGGCAATT CGTTGATGTA TATGGAATGG ATCCTGAACT CCTTAGCATG
GTACCAAGAC CAGTCTGTGC AGTCTTACTT CTCTTTCCTA TTACAGAAAA GTATGAAGTA
TTCAGAACAG AAGAGGAAGA AAAAATAAAA TCTCAGGGAC AAGATGTTAC ATCATCAGTA
TATTTCATGA AGCAAACAAT CAGCAATGCC TGTGGAACAA TTGGACTGAT TCATGCTATT
GCAAACAATA AAGACAAGAT GCACTTTGAA TCTGGATCAA CCTTGAAAAA ATTCCTGGAG
GAATCTGTGT CAATGAGCCC TGAAGAACGA GCCAGATACC TGGAGAACTA TGATGCCATC
CGAGTTACTC ATGAGACCAG TGCCCATGAA GGTCAGACTG AGTCCTCCTC GCCATCCTCA
TCACAGCCTC ATTCTAGCCA CTGCAGAACG AAGGCCTCAT CATTGTGTCA CCATGCATCC
CTGCCCTGGG TCAAACGTAA CCATGTAGGC CCTGCAAAGG CTACCAGTCC ATCTCTCCGT
CTTCGTCGCT GGCGACCCTT CTTACGTCTA CCATCTTTGG GTCTCCATTC TGTTGCACCA
AGTATAGATG AGAAAGTAGA TCTTCATTTT ATTGCATTAG TTCATGTAGA TGGGCATCTC
TATGAATTAG ATGGGCGGAA GCCATTTCCA ATTAACCATG GTGAAACTAG TGATGAAACT
TTATTAGAGG ATGCCATAGA AGTTTGCAAG AAGTTTATGG AGCGCGACCC TGATGAACTA
AGATTTAATG CGATTGCTCT TTCTGCAGCA TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011533515.1
CDS62..934
Misc Feature(1)77..922(+)
Misc Feature(2)83..880(+)
Misc Feature(3)83..748(+)
Misc Feature(4)314..793(+)
Translation
Position Chain Variation Link
7 7 -, ggcggcggcggcgaa dbSNP:149695719
13 13 a, g dbSNP:779137098
16 16 -, ggcgaaggcggcggc dbSNP:8192737
19 19 a, g dbSNP:750111627
21 21 -, ggcggcggcggcgaa dbSNP:769130927
25 25 g, t dbSNP:755762408
27 27 c, g, t dbSNP:765711293
34 34 c, t dbSNP:758831328
35 35 a, t dbSNP:778468100
36 36 a, c, g dbSNP:367636758
39 39 c, t dbSNP:757382777
42 42 g, t dbSNP:781089163
44 44 a, g dbSNP:746104640
47 47 c, t dbSNP:201923490
48 48 a, c, t dbSNP:199605560
52 52 c, t dbSNP:7996485
55 55 c, g dbSNP:528032236
56 56 a, g dbSNP:148862350
60 60 c, t dbSNP:138830150
62 62 a, t dbSNP:772541882
63 63 c, g, t dbSNP:760249794
68 68 a, g dbSNP:753514395
69 69 a, g dbSNP:758887565
71 71 c, g dbSNP:764320440
75 75 a, g dbSNP:535349329
96 96 a, g dbSNP:141970788
100 100 c, g dbSNP:781342676
106 106 g, t dbSNP:755293457
126 126 a, t dbSNP:781516708
130 130 c, t dbSNP:779043780
133 133 c, t dbSNP:752943510
134 134 c, t dbSNP:758153494
142 142 c, t dbSNP:192624215
143 143 a, g dbSNP:746951082
148 148 c, t dbSNP:752958414
150 150 c, t dbSNP:781258928
160 160 a, g dbSNP:745434848
166 166 c, t dbSNP:756325539
169 169 a, g dbSNP:111653662
173 173 c, t dbSNP:775053154
179 179 a, g dbSNP:372889994
184 184 a, g dbSNP:772354880
186 186 c, g dbSNP:773497877
199 199 c, t dbSNP:761015496
207 207 g, t dbSNP:146293310
238 238 a, c dbSNP:764474703
244 244 -, cagaa dbSNP:760217672
251 251 a, g dbSNP:553927686
265 265 a, t dbSNP:376728987
274 274 c, t dbSNP:767370396
279 279 g, t dbSNP:141870275
280 280 a, g dbSNP:756013143
287 287 a, g dbSNP:779443132
291 291 c, t dbSNP:753456567
294 294 c, t dbSNP:547414245
323 323 a, g dbSNP:754453843
327 327 a, g dbSNP:778587420
333 333 a, g dbSNP:747751143
342 342 c, t dbSNP:771320095
353 353 c, g dbSNP:781555331
373 373 a, g dbSNP:376626341
383 383 c, t dbSNP:572185099
394 394 c, t dbSNP:764743569
396 396 c, g dbSNP:200965579
398 398 c, t dbSNP:757903004
401 401 a, c, g dbSNP:12872971
411 411 a, g dbSNP:751145532
415 415 c, g dbSNP:756520815
422 422 a, g dbSNP:527890812
428 428 a, g, t dbSNP:749816740
430 430 a, g dbSNP:779548682
438 438 a, g dbSNP:748304370
449 449 c, t dbSNP:374305257
450 450 a, g dbSNP:11555620
463 463 a, g dbSNP:377416621
472 472 c, t dbSNP:747161304
478 478 c, t dbSNP:773674690
481 481 c, g dbSNP:761371610
482 482 c, t dbSNP:767343926
483 483 a, g dbSNP:750068505
492 492 a, g dbSNP:760002431
493 493 c, t dbSNP:765657943
524 524 -, tcc dbSNP:35701234
528 528 -, cct dbSNP:8192754
529 529 -, ctc dbSNP:68176896
529 529 c, g dbSNP:74094453
530 530 -, tcg dbSNP:199782321
531 531 c, t dbSNP:771659221
555 555 c, t dbSNP:556746768
570 570 c, t dbSNP:773031541
600 600 a, c dbSNP:578223221
606 606 c, t dbSNP:545089974
626 626 c, g dbSNP:765712894
653 653 c, t dbSNP:753063819
659 659 c, t dbSNP:763374002
665 665 c, t dbSNP:369614682
669 669 g, t dbSNP:764553436
675 675 a, g dbSNP:752087544
682 682 c, t dbSNP:553853730
686 686 c, g, t dbSNP:780661979
689 689 c, g dbSNP:757346726
719 719 c, t dbSNP:375314560
720 720 c, t dbSNP:772792014
723 723 a, g dbSNP:201962508
725 725 a, g dbSNP:770776286
727 727 a, g dbSNP:776318628
731 731 a, g dbSNP:200687621
735 735 a, g dbSNP:539792961
741 741 a, g dbSNP:764475282
745 745 a, t dbSNP:188855227
748 748 c, t dbSNP:371408920
760 760 a, g dbSNP:767594664
767 767 a, g dbSNP:750557779
775 775 a, g dbSNP:756180319
781 781 a, c dbSNP:770002848
792 792 a, g dbSNP:770547010
797 797 c, t dbSNP:776173957
811 811 -, a dbSNP:777059224
819 819 a, g dbSNP:745490042
832 832 c, t dbSNP:768902862
837 837 a, c dbSNP:774717261
839 839 a, g dbSNP:762150228
869 869 a, c, g dbSNP:752414958
870 870 a, g dbSNP:150677527
871 871 a, g dbSNP:147520161
880 880 -, g dbSNP:766467942
881 881 a, g dbSNP:772405094
882 882 a, c dbSNP:773730866
883 883 a, g dbSNP:761103437
884 884 c, t dbSNP:770928527
885 885 a, g dbSNP:776829672
886 886 c, t dbSNP:561552308
887 887 a, g dbSNP:765533437
892 892 c, t dbSNP:376497370
895 895 g, t dbSNP:760310101
912 912 c, t dbSNP:139878255
913 913 a, g dbSNP:763937455
920 920 c, g dbSNP:751552546
930 930 c, t dbSNP:757164158
931 931 a, g dbSNP:780980995
944 944 a, t dbSNP:750019599
945 945 a, g, t dbSNP:755549913
948 948 a, g dbSNP:748891906
966 966 c, t dbSNP:772458113
970 970 a, t dbSNP:371424841
985 985 c, g dbSNP:778053733
995 995 c, g dbSNP:532076425
1007 1007 a, c dbSNP:762029977
1022 1022 a, g dbSNP:550058570
1028 1028 g, t dbSNP:2274048

Target ORF information:

RefSeq Version XM_011535213
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535213

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGTTTCTTAA ACAATTAGGT 
CTACATCCTA ACTGGCAATT CGTTGATGTA TATGGAATGG ATCCTGAACT CCTTAGCATG
GTACCAAGAC CAGTCTGTGC AGTCTTACTT CTCTTTCCTA TTACAGAAAA GTATGAAGTA
TTCAGAACAG AAGAGGAAGA AAAAATAAAA TCTCAGGGAC AAGATGTTAC ATCATCAGTA
TATTTCATGA AGCAAACAAT CAGCAATGCC TGTGGAACAA TTGGACTGAT TCATGCTATT
GCAAACAATA AAGACAAGAT GCACTTTGAA TCTGGATCAA CCTTGAAAAA ATTCCTGGAG
GAATCTGTGT CAATGAGCCC TGAAGAACGA GCCAGATACC TGGAGAACTA TGATGCCATC
CGAGTTACTC ATGAGACCAG TGCCCATGAA GGTCAGACTG AGTCCTCCTC GCCATCCTCA
TCACAGCCTC ATTCTAGCCA CTGCAGAACG AAGGCCTCAT CATTGTGTCA CCATGCATCC
CTGCCCTGGG TCAAACGTAA CCATGTAGGC CCTGCAAAGG CTACCAGTCC ATCTCTCCGT
CTTCGTCGCT GGCGACCCTT CTTACGTCTA CCATCTTTGG GTCTCCATTC TGTTGCACCA
AGTATAGATG AGAAAGTAGA TCTTCATTTT ATTGCATTAG TTCATGTAGA TGGGCATCTC
TATGAATTAG ATGGGCGGAA GCCATTTCCA ATTAACCATG GTGAAACTAG TGATGAAACT
TTATTAGAGG ATGCCATAGA AGTTTGCAAG AAGTTTATGG AGCGCGACCC TGATGAACTA
AGATTTAATG CGATTGCTCT TTCTGCAGCA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61219
Accession Version XM_011535214.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 777bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGATCCTG AACTCCTTAG CATGGTACCA AGACCAGTCT GTGCAGTCTT ACTTCTCTTT 
CCTATTACAG AAAAGTATGA AGTATTCAGA ACAGAAGAGG AAGAAAAAAT AAAATCTCAG
GGACAAGATG TTACATCATC AGTATATTTC ATGAAGCAAA CAATCAGCAA TGCCTGTGGA
ACAATTGGAC TGATTCATGC TATTGCAAAC AATAAAGACA AGATGCACTT TGAATCTGGA
TCAACCTTGA AAAAATTCCT GGAGGAATCT GTGTCAATGA GCCCTGAAGA ACGAGCCAGA
TACCTGGAGA ACTATGATGC CATCCGAGTT ACTCATGAGA CCAGTGCCCA TGAAGGTCAG
ACTGAGTCCT CCTCGCCATC CTCATCACAG CCTCATTCTA GCCACTGCAG AACGAAGGCC
TCATCATTGT GTCACCATGC ATCCCTGCCC TGGGTCAAAC GTAACCATGT AGGCCCTGCA
AAGGCTACCA GTCCATCTCT CCGTCTTCGT CGCTGGCGAC CCTTCTTACG TCTACCATCT
TTGGGTCTCC ATTCTGTTGC ACCAAGTATA GATGAGAAAG TAGATCTTCA TTTTATTGCA
TTAGTTCATG TAGATGGGCA TCTCTATGAA TTAGATGGGC GGAAGCCATT TCCAATTAAC
CATGGTGAAA CTAGTGATGA AACTTTATTA GAGGATGCCA TAGAAGTTTG CAAGAAGTTT
ATGGAGCGCG ACCCTGATGA ACTAAGATTT AATGCGATTG CTCTTTCTGC AGCATAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011533516.1
CDS871..1647
Misc Feature(1)871..1635(+)
Misc Feature(2)1027..1506(+)
Misc Feature(3)1027..1461(+)
Translation
Position Chain Variation Link
25 25 c, t dbSNP:554598982
84 84 c, g dbSNP:9600493
112 112 c, g dbSNP:754110303
235 235 a, g dbSNP:757470853
288 288 c, g dbSNP:778925072
296 296 c, t dbSNP:3812846
316 316 a, g dbSNP:149834078
333 333 c, t dbSNP:768490131
347 347 c, t dbSNP:781116275
386 386 c, t dbSNP:35853105
404 404 a, t dbSNP:187174652
421 421 c, t dbSNP:769465288
444 444 c, g dbSNP:3812845
451 451 c, t dbSNP:560127877
454 454 c, t dbSNP:3812844
465 465 a, t dbSNP:762731654
528 528 a, g dbSNP:116535756
535 535 a, g dbSNP:569739707
539 539 c, t dbSNP:770606143
547 547 a, g dbSNP:749913833
549 549 c, t dbSNP:543151918
553 553 a, g dbSNP:530644948
575 575 a, g dbSNP:575042213
579 579 c, g dbSNP:773750152
597 597 c, g dbSNP:145801862
599 599 c, g dbSNP:570424309
601 601 a, g dbSNP:546440611
606 606 a, c dbSNP:759023184
607 607 a, g dbSNP:534361554
636 636 g, t dbSNP:552809726
638 638 a, t dbSNP:370778850
639 639 c, g, t dbSNP:7995889
646 646 g, t dbSNP:367633793
663 663 a, c dbSNP:535245498
673 673 g, t dbSNP:556755792
674 674 a, g dbSNP:575385099
676 676 a, g dbSNP:2281762
692 692 c, t dbSNP:558827747
695 695 a, g dbSNP:764484496
729 729 -, t dbSNP:112313138
730 730 -, t dbSNP:572753140
733 733 g, t dbSNP:61472085
738 738 c, t dbSNP:541444652
775 775 -, c dbSNP:754323498
775 775 c, t dbSNP:373126097
783 783 a, c, t dbSNP:4885310
794 794 c, t dbSNP:542407363
807 807 c, t dbSNP:569027565
819 819 g, t dbSNP:755293457
839 839 a, t dbSNP:781516708
843 843 c, t dbSNP:779043780
846 846 c, t dbSNP:752943510
847 847 c, t dbSNP:758153494
855 855 c, t dbSNP:192624215
856 856 a, g dbSNP:746951082
861 861 c, t dbSNP:752958414
863 863 c, t dbSNP:781258928
873 873 a, g dbSNP:745434848
879 879 c, t dbSNP:756325539
882 882 a, g dbSNP:111653662
886 886 c, t dbSNP:775053154
892 892 a, g dbSNP:372889994
897 897 a, g dbSNP:772354880
899 899 c, g dbSNP:773497877
912 912 c, t dbSNP:761015496
920 920 g, t dbSNP:146293310
951 951 a, c dbSNP:764474703
957 957 -, cagaa dbSNP:760217672
964 964 a, g dbSNP:553927686
978 978 a, t dbSNP:376728987
987 987 c, t dbSNP:767370396
992 992 g, t dbSNP:141870275
993 993 a, g dbSNP:756013143
1000 1000 a, g dbSNP:779443132
1004 1004 c, t dbSNP:753456567
1007 1007 c, t dbSNP:547414245
1036 1036 a, g dbSNP:754453843
1040 1040 a, g dbSNP:778587420
1046 1046 a, g dbSNP:747751143
1055 1055 c, t dbSNP:771320095
1066 1066 c, g dbSNP:781555331
1086 1086 a, g dbSNP:376626341
1096 1096 c, t dbSNP:572185099
1107 1107 c, t dbSNP:764743569
1109 1109 c, g dbSNP:200965579
1111 1111 c, t dbSNP:757903004
1114 1114 a, c, g dbSNP:12872971
1124 1124 a, g dbSNP:751145532
1128 1128 c, g dbSNP:756520815
1135 1135 a, g dbSNP:527890812
1141 1141 a, g, t dbSNP:749816740
1143 1143 a, g dbSNP:779548682
1151 1151 a, g dbSNP:748304370
1162 1162 c, t dbSNP:374305257
1163 1163 a, g dbSNP:11555620
1176 1176 a, g dbSNP:377416621
1185 1185 c, t dbSNP:747161304
1191 1191 c, t dbSNP:773674690
1194 1194 c, g dbSNP:761371610
1195 1195 c, t dbSNP:767343926
1196 1196 a, g dbSNP:750068505
1205 1205 a, g dbSNP:760002431
1206 1206 c, t dbSNP:765657943
1237 1237 -, tcc dbSNP:35701234
1241 1241 -, cct dbSNP:8192754
1242 1242 -, ctc dbSNP:68176896
1242 1242 c, g dbSNP:74094453
1243 1243 -, tcg dbSNP:199782321
1244 1244 c, t dbSNP:771659221
1268 1268 c, t dbSNP:556746768
1283 1283 c, t dbSNP:773031541
1313 1313 a, c dbSNP:578223221
1319 1319 c, t dbSNP:545089974
1339 1339 c, g dbSNP:765712894
1366 1366 c, t dbSNP:753063819
1372 1372 c, t dbSNP:763374002
1378 1378 c, t dbSNP:369614682
1382 1382 g, t dbSNP:764553436
1388 1388 a, g dbSNP:752087544
1395 1395 c, t dbSNP:553853730
1399 1399 c, g, t dbSNP:780661979
1402 1402 c, g dbSNP:757346726
1432 1432 c, t dbSNP:375314560
1433 1433 c, t dbSNP:772792014
1436 1436 a, g dbSNP:201962508
1438 1438 a, g dbSNP:770776286
1440 1440 a, g dbSNP:776318628
1444 1444 a, g dbSNP:200687621
1448 1448 a, g dbSNP:539792961
1454 1454 a, g dbSNP:764475282
1458 1458 a, t dbSNP:188855227
1461 1461 c, t dbSNP:371408920
1473 1473 a, g dbSNP:767594664
1480 1480 a, g dbSNP:750557779
1488 1488 a, g dbSNP:756180319
1494 1494 a, c dbSNP:770002848
1505 1505 a, g dbSNP:770547010
1510 1510 c, t dbSNP:776173957
1524 1524 -, a dbSNP:777059224
1532 1532 a, g dbSNP:745490042
1545 1545 c, t dbSNP:768902862
1550 1550 a, c dbSNP:774717261
1552 1552 a, g dbSNP:762150228
1582 1582 a, c, g dbSNP:752414958
1583 1583 a, g dbSNP:150677527
1584 1584 a, g dbSNP:147520161
1593 1593 -, g dbSNP:766467942
1594 1594 a, g dbSNP:772405094
1595 1595 a, c dbSNP:773730866
1596 1596 a, g dbSNP:761103437
1597 1597 c, t dbSNP:770928527
1598 1598 a, g dbSNP:776829672
1599 1599 c, t dbSNP:561552308
1600 1600 a, g dbSNP:765533437
1605 1605 c, t dbSNP:376497370
1608 1608 g, t dbSNP:760310101
1625 1625 c, t dbSNP:139878255
1626 1626 a, g dbSNP:763937455
1633 1633 c, g dbSNP:751552546
1643 1643 c, t dbSNP:757164158
1644 1644 a, g dbSNP:780980995
1657 1657 a, t dbSNP:750019599
1658 1658 a, g, t dbSNP:755549913
1661 1661 a, g dbSNP:748891906
1679 1679 c, t dbSNP:772458113
1683 1683 a, t dbSNP:371424841
1698 1698 c, g dbSNP:778053733
1708 1708 c, g dbSNP:532076425
1720 1720 a, c dbSNP:762029977
1735 1735 a, g dbSNP:550058570
1741 1741 g, t dbSNP:2274048

Target ORF information:

RefSeq Version XM_011535214
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535214

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGATCCTG AACTCCTTAG CATGGTACCA AGACCAGTCT GTGCAGTCTT ACTTCTCTTT 
CCTATTACAG AAAAGTATGA AGTATTCAGA ACAGAAGAGG AAGAAAAAAT AAAATCTCAG
GGACAAGATG TTACATCATC AGTATATTTC ATGAAGCAAA CAATCAGCAA TGCCTGTGGA
ACAATTGGAC TGATTCATGC TATTGCAAAC AATAAAGACA AGATGCACTT TGAATCTGGA
TCAACCTTGA AAAAATTCCT GGAGGAATCT GTGTCAATGA GCCCTGAAGA ACGAGCCAGA
TACCTGGAGA ACTATGATGC CATCCGAGTT ACTCATGAGA CCAGTGCCCA TGAAGGTCAG
ACTGAGTCCT CCTCGCCATC CTCATCACAG CCTCATTCTA GCCACTGCAG AACGAAGGCC
TCATCATTGT GTCACCATGC ATCCCTGCCC TGGGTCAAAC GTAACCATGT AGGCCCTGCA
AAGGCTACCA GTCCATCTCT CCGTCTTCGT CGCTGGCGAC CCTTCTTACG TCTACCATCT
TTGGGTCTCC ATTCTGTTGC ACCAAGTATA GATGAGAAAG TAGATCTTCA TTTTATTGCA
TTAGTTCATG TAGATGGGCA TCTCTATGAA TTAGATGGGC GGAAGCCATT TCCAATTAAC
CATGGTGAAA CTAGTGATGA AACTTTATTA GAGGATGCCA TAGAAGTTTG CAAGAAGTTT
ATGGAGCGCG ACCCTGATGA ACTAAGATTT AATGCGATTG CTCTTTCTGC AGCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10261
Accession Version NM_001270952.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL137244.28, BQ427804.1, BC018125.2 and AA234624.1. Summary: The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]. Transcript Variant: This variant (1) represents the longer transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU945230.1, AA746355.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGATCCTG AACTCCTTAG CATGGTACCA AGACCAGTCT GTGCAGTCTT ACTTCTCTTT 
CCTATTACAG AAAAGTATGA AGTATTCAGA ACAGAAGAGG AAGAAAAAAT AAAATCTCAG
GGACAAGATG TTACATCATC AGTATATTTC ATGAAGCAAA CAATCAGCAA TGCCTGTGGA
ACAATTGGAC TGATTCATGC TATTGCAAAC AATAAAGACA AGATGCACTT TGAATCTGGA
TCAACCTTGA AAAAATTCCT GGAGGAATCT GTGTCAATGA GCCCTGAAGA ACGAGCCAGA
TACCTGGAGA ACTATGATGC CATCCGAGTT ACTCATGAGA CCAGTGCCCA TGAAGGTCAG
ACTGAGGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGATGGGCGG AAGCCATTTC CAATTAACCA TGGTGAAACT
AGTGATGAAA CTTTATTAGA GGATGCCATA GAAGTTTGCA AGAAGTTTAT GGAGCGCGAC
CCTGATGAAC TAAGATTTAA TGCGATTGCT CTTTCTGCAG CATAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001257881.1
CDS258..842
Misc Feature(1)159..161(+)
Misc Feature(2)258..830(+)
Misc Feature(3)414..701(+)
Misc Feature(4)414..656(+)
Exon (1)1..191
Gene:UCHL3
Gene Synonym:
Exon (2)192..203
Gene:UCHL3
Gene Synonym:
Exon (3)204..332
Gene:UCHL3
Gene Synonym:
Exon (4)333..489
Gene:UCHL3
Gene Synonym:
Exon (5)490..575
Gene:UCHL3
Gene Synonym:
Exon (6)576..623
Gene:UCHL3
Gene Synonym:
Exon (7)624..699
Gene:UCHL3
Gene Synonym:
Exon (8)700..758
Gene:UCHL3
Gene Synonym:
Exon (9)759..1050
Gene:UCHL3
Gene Synonym:
Translation
Position Chain Variation Link
23 23 g, t dbSNP:552809726
25 25 a, t dbSNP:370778850
26 26 c, g, t dbSNP:7995889
33 33 g, t dbSNP:367633793
50 50 a, c dbSNP:535245498
60 60 g, t dbSNP:556755792
61 61 a, g dbSNP:575385099
63 63 a, g dbSNP:2281762
79 79 c, t dbSNP:558827747
82 82 a, g dbSNP:764484496
116 116 -, t dbSNP:112313138
117 117 -, t dbSNP:572753140
120 120 g, t dbSNP:61472085
125 125 c, t dbSNP:541444652
162 162 -, c dbSNP:754323498
162 162 c, t dbSNP:373126097
170 170 a, c, t dbSNP:4885310
181 181 c, t dbSNP:542407363
194 194 c, t dbSNP:569027565
206 206 g, t dbSNP:755293457
226 226 a, t dbSNP:781516708
230 230 c, t dbSNP:779043780
233 233 c, t dbSNP:752943510
234 234 c, t dbSNP:758153494
242 242 c, t dbSNP:192624215
243 243 a, g dbSNP:746951082
248 248 c, t dbSNP:752958414
250 250 c, t dbSNP:781258928
260 260 a, g dbSNP:745434848
266 266 c, t dbSNP:756325539
269 269 a, g dbSNP:111653662
273 273 c, t dbSNP:775053154
279 279 a, g dbSNP:372889994
284 284 a, g dbSNP:772354880
286 286 c, g dbSNP:773497877
299 299 c, t dbSNP:761015496
307 307 g, t dbSNP:146293310
338 338 a, c dbSNP:764474703
344 344 -, cagaa dbSNP:760217672
351 351 a, g dbSNP:553927686
365 365 a, t dbSNP:376728987
374 374 c, t dbSNP:767370396
379 379 g, t dbSNP:141870275
380 380 a, g dbSNP:756013143
387 387 a, g dbSNP:779443132
391 391 c, t dbSNP:753456567
394 394 c, t dbSNP:547414245
423 423 a, g dbSNP:754453843
427 427 a, g dbSNP:778587420
433 433 a, g dbSNP:747751143
442 442 c, t dbSNP:771320095
453 453 c, g dbSNP:781555331
473 473 a, g dbSNP:376626341
483 483 c, t dbSNP:572185099
494 494 c, t dbSNP:764743569
496 496 c, g dbSNP:200965579
498 498 c, t dbSNP:757903004
501 501 a, c, g dbSNP:12872971
511 511 a, g dbSNP:751145532
515 515 c, g dbSNP:756520815
522 522 a, g dbSNP:527890812
528 528 a, g, t dbSNP:749816740
530 530 a, g dbSNP:779548682
538 538 a, g dbSNP:748304370
549 549 c, t dbSNP:374305257
550 550 a, g dbSNP:11555620
563 563 a, g dbSNP:377416621
572 572 c, t dbSNP:747161304
578 578 c, t dbSNP:773674690
581 581 c, g dbSNP:761371610
582 582 c, t dbSNP:767343926
583 583 a, g dbSNP:750068505
592 592 a, g dbSNP:760002431
593 593 c, t dbSNP:765657943
627 627 c, t dbSNP:375314560
628 628 c, t dbSNP:772792014
631 631 a, g dbSNP:201962508
633 633 a, g dbSNP:770776286
635 635 a, g dbSNP:776318628
639 639 a, g dbSNP:200687621
643 643 a, g dbSNP:539792961
649 649 a, g dbSNP:764475282
653 653 a, t dbSNP:188855227
656 656 c, t dbSNP:371408920
668 668 a, g dbSNP:767594664
675 675 a, g dbSNP:750557779
683 683 a, g dbSNP:756180319
689 689 a, c dbSNP:770002848
700 700 a, g dbSNP:770547010
705 705 c, t dbSNP:776173957
719 719 -, a dbSNP:777059224
727 727 a, g dbSNP:745490042
740 740 c, t dbSNP:768902862
745 745 a, c dbSNP:774717261
747 747 a, g dbSNP:762150228
777 777 a, c, g dbSNP:752414958
778 778 a, g dbSNP:150677527
779 779 a, g dbSNP:147520161
788 788 -, g dbSNP:766467942
789 789 a, g dbSNP:772405094
790 790 a, c dbSNP:773730866
791 791 a, g dbSNP:761103437
792 792 c, t dbSNP:770928527
793 793 a, g dbSNP:776829672
794 794 c, t dbSNP:561552308
795 795 a, g dbSNP:765533437
800 800 c, t dbSNP:376497370
803 803 g, t dbSNP:760310101
820 820 c, t dbSNP:139878255
821 821 a, g dbSNP:763937455
828 828 c, g dbSNP:751552546
838 838 c, t dbSNP:757164158
839 839 a, g dbSNP:780980995
852 852 a, t dbSNP:750019599
853 853 a, g, t dbSNP:755549913
856 856 a, g dbSNP:748891906
874 874 c, t dbSNP:772458113
878 878 a, t dbSNP:371424841
893 893 c, g dbSNP:778053733
903 903 c, g dbSNP:532076425
915 915 a, c dbSNP:762029977
930 930 a, g dbSNP:550058570
936 936 g, t dbSNP:2274048
977 977 a, t dbSNP:753348223
1011 1011 c, t dbSNP:112597363
1018 1018 c, t dbSNP:532073619
1043 1043 a, t dbSNP:757726253

Target ORF information:

RefSeq Version NM_001270952
Organism Homo sapiens (human)
Definition Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001270952

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGATCCTG AACTCCTTAG CATGGTACCA AGACCAGTCT GTGCAGTCTT ACTTCTCTTT 
CCTATTACAG AAAAGTATGA AGTATTCAGA ACAGAAGAGG AAGAAAAAAT AAAATCTCAG
GGACAAGATG TTACATCATC AGTATATTTC ATGAAGCAAA CAATCAGCAA TGCCTGTGGA
ACAATTGGAC TGATTCATGC TATTGCAAAC AATAAAGACA AGATGCACTT TGAATCTGGA
TCAACCTTGA AAAAATTCCT GGAGGAATCT GTGTCAATGA GCCCTGAAGA ACGAGCCAGA
TACCTGGAGA ACTATGATGC CATCCGAGTT ACTCATGAGA CCAGTGCCCA TGAAGGTCAG
ACTGAGGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGATGGGCGG AAGCCATTTC CAATTAACCA TGGTGAAACT
AGTGATGAAA CTTTATTAGA GGATGCCATA GAAGTTTGCA AGAAGTTTAT GGAGCGCGAC
CCTGATGAAC TAAGATTTAA TGCGATTGCT CTTTCTGCAG CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56052
Accession Version NM_006002.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 693bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA820150.1, BC018125.2 and AA234624.1. On Aug 18, 2012 this sequence version replaced gi:37059734. Summary: The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]. Transcript Variant: This variant (2) contains an alternate exon in the 5' coding region and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI602995.1, BC018125.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
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121
181
241
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361
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481
541
601
661
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGGCACCA
AGTATAGATG AGAAAGTAGA TCTTCATTTT ATTGCATTAG TTCATGTAGA TGGGCATCTC
TATGAATTAG ATGGGCGGAA GCCATTTCCA ATTAACCATG GTGAAACTAG TGATGAAACT
TTATTAGAGG ATGCCATAGA AGTTTGCAAG AAGTTTATGG AGCGCGACCC TGATGAACTA
AGATTTAATG CGATTGCTCT TTCTGCAGCA TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_005993.1
CDS72..764
Misc Feature(1)87..752(+)
Misc Feature(2)93..710(+)
Misc Feature(3)93..578(+)
Misc Feature(4)93..110(+)
Misc Feature(5)294..296(+)
Misc Feature(6)336..623(+)
Misc Feature(7)459..461(+)
Misc Feature(8)525..548(+)
Misc Feature(9)621..623(+)
Misc Feature(10)726..743(+)
Exon (1)1..113
Gene:UCHL3
Gene Synonym:
Exon (2)114..125
Gene:UCHL3
Gene Synonym:
Exon (3)126..254
Gene:UCHL3
Gene Synonym:
Exon (4)255..411
Gene:UCHL3
Gene Synonym:
Exon (5)412..497
Gene:UCHL3
Gene Synonym:
Exon (6)498..545
Gene:UCHL3
Gene Synonym:
Exon (7)546..621
Gene:UCHL3
Gene Synonym:
Exon (8)622..680
Gene:UCHL3
Gene Synonym:
Exon (9)681..972
Gene:UCHL3
Gene Synonym:
Translation
Position Chain Variation Link
17 17 -, ggcggcggcggcgaa dbSNP:149695719
23 23 a, g dbSNP:779137098
26 26 -, ggcgaaggcggcggc dbSNP:8192737
29 29 a, g dbSNP:750111627
31 31 -, ggcggcggcggcgaa dbSNP:769130927
35 35 g, t dbSNP:755762408
37 37 c, g, t dbSNP:765711293
44 44 c, t dbSNP:758831328
45 45 a, t dbSNP:778468100
46 46 a, c, g dbSNP:367636758
49 49 c, t dbSNP:757382777
52 52 g, t dbSNP:781089163
54 54 a, g dbSNP:746104640
57 57 c, t dbSNP:201923490
58 58 a, c, t dbSNP:199605560
62 62 c, t dbSNP:7996485
65 65 c, g dbSNP:528032236
66 66 a, g dbSNP:148862350
70 70 c, t dbSNP:138830150
72 72 a, t dbSNP:772541882
73 73 c, g, t dbSNP:760249794
78 78 a, g dbSNP:753514395
79 79 a, g dbSNP:758887565
81 81 c, g dbSNP:764320440
85 85 a, g dbSNP:535349329
106 106 a, g dbSNP:141970788
110 110 c, g dbSNP:781342676
116 116 c, t dbSNP:569027565
128 128 g, t dbSNP:755293457
148 148 a, t dbSNP:781516708
152 152 c, t dbSNP:779043780
155 155 c, t dbSNP:752943510
156 156 c, t dbSNP:758153494
164 164 c, t dbSNP:192624215
165 165 a, g dbSNP:746951082
170 170 c, t dbSNP:752958414
172 172 c, t dbSNP:781258928
182 182 a, g dbSNP:745434848
188 188 c, t dbSNP:756325539
191 191 a, g dbSNP:111653662
195 195 c, t dbSNP:775053154
201 201 a, g dbSNP:372889994
206 206 a, g dbSNP:772354880
208 208 c, g dbSNP:773497877
221 221 c, t dbSNP:761015496
229 229 g, t dbSNP:146293310
260 260 a, c dbSNP:764474703
266 266 -, cagaa dbSNP:760217672
273 273 a, g dbSNP:553927686
287 287 a, t dbSNP:376728987
296 296 c, t dbSNP:767370396
301 301 g, t dbSNP:141870275
302 302 a, g dbSNP:756013143
309 309 a, g dbSNP:779443132
313 313 c, t dbSNP:753456567
316 316 c, t dbSNP:547414245
345 345 a, g dbSNP:754453843
349 349 a, g dbSNP:778587420
355 355 a, g dbSNP:747751143
364 364 c, t dbSNP:771320095
375 375 c, g dbSNP:781555331
395 395 a, g dbSNP:376626341
405 405 c, t dbSNP:572185099
416 416 c, t dbSNP:764743569
418 418 c, g dbSNP:200965579
420 420 c, t dbSNP:757903004
423 423 a, c, g dbSNP:12872971
433 433 a, g dbSNP:751145532
437 437 c, g dbSNP:756520815
444 444 a, g dbSNP:527890812
450 450 a, g, t dbSNP:749816740
452 452 a, g dbSNP:779548682
460 460 a, g dbSNP:748304370
471 471 c, t dbSNP:374305257
472 472 a, g dbSNP:11555620
485 485 a, g dbSNP:377416621
494 494 c, t dbSNP:747161304
500 500 c, t dbSNP:773674690
503 503 c, g dbSNP:761371610
504 504 c, t dbSNP:767343926
505 505 a, g dbSNP:750068505
514 514 a, g dbSNP:760002431
515 515 c, t dbSNP:765657943
549 549 c, t dbSNP:375314560
550 550 c, t dbSNP:772792014
553 553 a, g dbSNP:201962508
555 555 a, g dbSNP:770776286
557 557 a, g dbSNP:776318628
561 561 a, g dbSNP:200687621
565 565 a, g dbSNP:539792961
571 571 a, g dbSNP:764475282
575 575 a, t dbSNP:188855227
578 578 c, t dbSNP:371408920
590 590 a, g dbSNP:767594664
597 597 a, g dbSNP:750557779
605 605 a, g dbSNP:756180319
611 611 a, c dbSNP:770002848
622 622 a, g dbSNP:770547010
627 627 c, t dbSNP:776173957
641 641 -, a dbSNP:777059224
649 649 a, g dbSNP:745490042
662 662 c, t dbSNP:768902862
667 667 a, c dbSNP:774717261
669 669 a, g dbSNP:762150228
699 699 a, c, g dbSNP:752414958
700 700 a, g dbSNP:150677527
701 701 a, g dbSNP:147520161
710 710 -, g dbSNP:766467942
711 711 a, g dbSNP:772405094
712 712 a, c dbSNP:773730866
713 713 a, g dbSNP:761103437
714 714 c, t dbSNP:770928527
715 715 a, g dbSNP:776829672
716 716 c, t dbSNP:561552308
717 717 a, g dbSNP:765533437
722 722 c, t dbSNP:376497370
725 725 g, t dbSNP:760310101
742 742 c, t dbSNP:139878255
743 743 a, g dbSNP:763937455
750 750 c, g dbSNP:751552546
760 760 c, t dbSNP:757164158
761 761 a, g dbSNP:780980995
774 774 a, t dbSNP:750019599
775 775 a, g, t dbSNP:755549913
778 778 a, g dbSNP:748891906
796 796 c, t dbSNP:772458113
800 800 a, t dbSNP:371424841
815 815 c, g dbSNP:778053733
825 825 c, g dbSNP:532076425
837 837 a, c dbSNP:762029977
852 852 a, g dbSNP:550058570
858 858 g, t dbSNP:2274048
899 899 a, t dbSNP:753348223
933 933 c, t dbSNP:112597363
940 940 c, t dbSNP:532073619
965 965 a, t dbSNP:757726253

Target ORF information:

RefSeq Version NM_006002
Organism Homo sapiens (human)
Definition Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006002

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGGCACCA
AGTATAGATG AGAAAGTAGA TCTTCATTTT ATTGCATTAG TTCATGTAGA TGGGCATCTC
TATGAATTAG ATGGGCGGAA GCCATTTCCA ATTAACCATG GTGAAACTAG TGATGAAACT
TTATTAGAGG ATGCCATAGA AGTTTGCAAG AAGTTTATGG AGCGCGACCC TGATGAACTA
AGATTTAATG CGATTGCTCT TTCTGCAGCA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.