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UCHL3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol UCHL3
Entrez Gene ID 7347
Full Name ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Synonyms UCH-L3
General protein information
Preferred Names
ubiquitin carboxyl-terminal hydrolase isozyme L3
Names
ubiquitin carboxyl-terminal hydrolase isozyme L3
ubiquitin thiolesterase
ubiquitin thioesterase L3
NP_001257881.1
NP_005993.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

13

13q22.2

Summary The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]. lac of sum
Disorder MIM:

603090


mRNA and Protein(s)

mRNA Protein Name
XM_011535212 XP_011533514 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X1
XM_011535213 XP_011533515 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X2
XM_011535214 XP_011533516 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X3
NM_001270952 NP_001257881 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 1
NM_006002 NP_005993 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 2



Homo sapiens (human) UCHL3 NP_005993.1
Pan troglodytes (chimpanzee) UCHL3 XP_003314228.1
Macaca mulatta (Rhesus monkey) UCHL3 XP_001085879.2
Canis lupus familiaris (dog) UCHL3 XP_534147.3
Bos taurus (cattle) UCHL3 NP_001035631.1
Mus musculus (house mouse) Uchl3 NP_057932.2
Rattus norvegicus (Norway rat) Uchl3 NP_001103635.1
Gallus gallus (chicken) UCHL3 NP_990156.1
Danio rerio (zebrafish) uchl3 NP_001019576.1
Drosophila melanogaster (fruit fly) Uch NP_001188681.1
Arabidopsis thaliana (thale cress) UCH3 NP_193484.1
Xenopus (Silurana) tropicalis (western clawed frog) uchl3 NP_001017120.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following UCHL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UCHL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu61217 XM_011535212 PREDICTED: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu61218 XM_011535213 PREDICTED: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu61219 XM_011535214 PREDICTED: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu10261
NM_001270952 Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu56052
NM_006002 Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu61217
Clone ID Related Accession (Same CDS sequence) XM_011535212
Accession Version XM_011535212.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 885bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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181
241
301
361
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481
541
601
661
721
781
841
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGTCCTCC
TCGCCATCCT CATCACAGCC TCATTCTAGC CACTGCAGAA CGAAGGCCTC ATCATTGTGT
CACCATGCAT CCCTGCCCTG GGTCAAACGT AACCATGTAG GCCCTGCAAA GGCTACCAGT
CCATCTCTCC GTCTTCGTCG CTGGCGACCC TTCTTACGTC TACCATCTTT GGGTCTCCAT
TCTGTTGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGATGGGCGG AAGCCATTTC CAATTAACCA TGGTGAAACT
AGTGATGAAA CTTTATTAGA GGATGCCATA GAAGTTTGCA AGAAGTTTAT GGAGCGCGAC
CCTGATGAAC TAAGATTTAA TGCGATTGCT CTTTCTGCAG CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533514.1
CDS72..956
Misc Feature(1)87..944(+)
Misc Feature(2)93..902(+)
Misc Feature(3)93..770(+)
Misc Feature(4)336..815(+)
Translation

Target ORF information:

RefSeq Version XM_011535212
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535212

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGTCCTCC
TCGCCATCCT CATCACAGCC TCATTCTAGC CACTGCAGAA CGAAGGCCTC ATCATTGTGT
CACCATGCAT CCCTGCCCTG GGTCAAACGT AACCATGTAG GCCCTGCAAA GGCTACCAGT
CCATCTCTCC GTCTTCGTCG CTGGCGACCC TTCTTACGTC TACCATCTTT GGGTCTCCAT
TCTGTTGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGATGGGCGG AAGCCATTTC CAATTAACCA TGGTGAAACT
AGTGATGAAA CTTTATTAGA GGATGCCATA GAAGTTTGCA AGAAGTTTAT GGAGCGCGAC
CCTGATGAAC TAAGATTTAA TGCGATTGCT CTTTCTGCAG CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61218
Clone ID Related Accession (Same CDS sequence) XM_011535213
Accession Version XM_011535213.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 873bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGTTTCTTAA ACAATTAGGT 
CTACATCCTA ACTGGCAATT CGTTGATGTA TATGGAATGG ATCCTGAACT CCTTAGCATG
GTACCAAGAC CAGTCTGTGC AGTCTTACTT CTCTTTCCTA TTACAGAAAA GTATGAAGTA
TTCAGAACAG AAGAGGAAGA AAAAATAAAA TCTCAGGGAC AAGATGTTAC ATCATCAGTA
TATTTCATGA AGCAAACAAT CAGCAATGCC TGTGGAACAA TTGGACTGAT TCATGCTATT
GCAAACAATA AAGACAAGAT GCACTTTGAA TCTGGATCAA CCTTGAAAAA ATTCCTGGAG
GAATCTGTGT CAATGAGCCC TGAAGAACGA GCCAGATACC TGGAGAACTA TGATGCCATC
CGAGTTACTC ATGAGACCAG TGCCCATGAA GGTCAGACTG AGTCCTCCTC GCCATCCTCA
TCACAGCCTC ATTCTAGCCA CTGCAGAACG AAGGCCTCAT CATTGTGTCA CCATGCATCC
CTGCCCTGGG TCAAACGTAA CCATGTAGGC CCTGCAAAGG CTACCAGTCC ATCTCTCCGT
CTTCGTCGCT GGCGACCCTT CTTACGTCTA CCATCTTTGG GTCTCCATTC TGTTGCACCA
AGTATAGATG AGAAAGTAGA TCTTCATTTT ATTGCATTAG TTCATGTAGA TGGGCATCTC
TATGAATTAG ATGGGCGGAA GCCATTTCCA ATTAACCATG GTGAAACTAG TGATGAAACT
TTATTAGAGG ATGCCATAGA AGTTTGCAAG AAGTTTATGG AGCGCGACCC TGATGAACTA
AGATTTAATG CGATTGCTCT TTCTGCAGCA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533515.1
CDS62..934
Misc Feature(1)77..922(+)
Misc Feature(2)83..880(+)
Misc Feature(3)83..748(+)
Misc Feature(4)314..793(+)
Translation

Target ORF information:

RefSeq Version XM_011535213
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535213

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGTTTCTTAA ACAATTAGGT 
CTACATCCTA ACTGGCAATT CGTTGATGTA TATGGAATGG ATCCTGAACT CCTTAGCATG
GTACCAAGAC CAGTCTGTGC AGTCTTACTT CTCTTTCCTA TTACAGAAAA GTATGAAGTA
TTCAGAACAG AAGAGGAAGA AAAAATAAAA TCTCAGGGAC AAGATGTTAC ATCATCAGTA
TATTTCATGA AGCAAACAAT CAGCAATGCC TGTGGAACAA TTGGACTGAT TCATGCTATT
GCAAACAATA AAGACAAGAT GCACTTTGAA TCTGGATCAA CCTTGAAAAA ATTCCTGGAG
GAATCTGTGT CAATGAGCCC TGAAGAACGA GCCAGATACC TGGAGAACTA TGATGCCATC
CGAGTTACTC ATGAGACCAG TGCCCATGAA GGTCAGACTG AGTCCTCCTC GCCATCCTCA
TCACAGCCTC ATTCTAGCCA CTGCAGAACG AAGGCCTCAT CATTGTGTCA CCATGCATCC
CTGCCCTGGG TCAAACGTAA CCATGTAGGC CCTGCAAAGG CTACCAGTCC ATCTCTCCGT
CTTCGTCGCT GGCGACCCTT CTTACGTCTA CCATCTTTGG GTCTCCATTC TGTTGCACCA
AGTATAGATG AGAAAGTAGA TCTTCATTTT ATTGCATTAG TTCATGTAGA TGGGCATCTC
TATGAATTAG ATGGGCGGAA GCCATTTCCA ATTAACCATG GTGAAACTAG TGATGAAACT
TTATTAGAGG ATGCCATAGA AGTTTGCAAG AAGTTTATGG AGCGCGACCC TGATGAACTA
AGATTTAATG CGATTGCTCT TTCTGCAGCA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61219
Clone ID Related Accession (Same CDS sequence) XM_011535214
Accession Version XM_011535214.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 777bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGATCCTG AACTCCTTAG CATGGTACCA AGACCAGTCT GTGCAGTCTT ACTTCTCTTT 
CCTATTACAG AAAAGTATGA AGTATTCAGA ACAGAAGAGG AAGAAAAAAT AAAATCTCAG
GGACAAGATG TTACATCATC AGTATATTTC ATGAAGCAAA CAATCAGCAA TGCCTGTGGA
ACAATTGGAC TGATTCATGC TATTGCAAAC AATAAAGACA AGATGCACTT TGAATCTGGA
TCAACCTTGA AAAAATTCCT GGAGGAATCT GTGTCAATGA GCCCTGAAGA ACGAGCCAGA
TACCTGGAGA ACTATGATGC CATCCGAGTT ACTCATGAGA CCAGTGCCCA TGAAGGTCAG
ACTGAGTCCT CCTCGCCATC CTCATCACAG CCTCATTCTA GCCACTGCAG AACGAAGGCC
TCATCATTGT GTCACCATGC ATCCCTGCCC TGGGTCAAAC GTAACCATGT AGGCCCTGCA
AAGGCTACCA GTCCATCTCT CCGTCTTCGT CGCTGGCGAC CCTTCTTACG TCTACCATCT
TTGGGTCTCC ATTCTGTTGC ACCAAGTATA GATGAGAAAG TAGATCTTCA TTTTATTGCA
TTAGTTCATG TAGATGGGCA TCTCTATGAA TTAGATGGGC GGAAGCCATT TCCAATTAAC
CATGGTGAAA CTAGTGATGA AACTTTATTA GAGGATGCCA TAGAAGTTTG CAAGAAGTTT
ATGGAGCGCG ACCCTGATGA ACTAAGATTT AATGCGATTG CTCTTTCTGC AGCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533516.1
CDS871..1647
Misc Feature(1)871..1635(+)
Misc Feature(2)1027..1506(+)
Misc Feature(3)1027..1461(+)
Translation

Target ORF information:

RefSeq Version XM_011535214
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535214

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGATCCTG AACTCCTTAG CATGGTACCA AGACCAGTCT GTGCAGTCTT ACTTCTCTTT 
CCTATTACAG AAAAGTATGA AGTATTCAGA ACAGAAGAGG AAGAAAAAAT AAAATCTCAG
GGACAAGATG TTACATCATC AGTATATTTC ATGAAGCAAA CAATCAGCAA TGCCTGTGGA
ACAATTGGAC TGATTCATGC TATTGCAAAC AATAAAGACA AGATGCACTT TGAATCTGGA
TCAACCTTGA AAAAATTCCT GGAGGAATCT GTGTCAATGA GCCCTGAAGA ACGAGCCAGA
TACCTGGAGA ACTATGATGC CATCCGAGTT ACTCATGAGA CCAGTGCCCA TGAAGGTCAG
ACTGAGTCCT CCTCGCCATC CTCATCACAG CCTCATTCTA GCCACTGCAG AACGAAGGCC
TCATCATTGT GTCACCATGC ATCCCTGCCC TGGGTCAAAC GTAACCATGT AGGCCCTGCA
AAGGCTACCA GTCCATCTCT CCGTCTTCGT CGCTGGCGAC CCTTCTTACG TCTACCATCT
TTGGGTCTCC ATTCTGTTGC ACCAAGTATA GATGAGAAAG TAGATCTTCA TTTTATTGCA
TTAGTTCATG TAGATGGGCA TCTCTATGAA TTAGATGGGC GGAAGCCATT TCCAATTAAC
CATGGTGAAA CTAGTGATGA AACTTTATTA GAGGATGCCA TAGAAGTTTG CAAGAAGTTT
ATGGAGCGCG ACCCTGATGA ACTAAGATTT AATGCGATTG CTCTTTCTGC AGCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10261
Clone ID Related Accession (Same CDS sequence) NM_001270952
Accession Version NM_001270952.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL137244.28, BQ427804.1, BC018125.2 and AA234624.1. Transcript Variant: This variant (1) represents the longer transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU945230.1, AA746355.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGATCCTG AACTCCTTAG CATGGTACCA AGACCAGTCT GTGCAGTCTT ACTTCTCTTT 
CCTATTACAG AAAAGTATGA AGTATTCAGA ACAGAAGAGG AAGAAAAAAT AAAATCTCAG
GGACAAGATG TTACATCATC AGTATATTTC ATGAAGCAAA CAATCAGCAA TGCCTGTGGA
ACAATTGGAC TGATTCATGC TATTGCAAAC AATAAAGACA AGATGCACTT TGAATCTGGA
TCAACCTTGA AAAAATTCCT GGAGGAATCT GTGTCAATGA GCCCTGAAGA ACGAGCCAGA
TACCTGGAGA ACTATGATGC CATCCGAGTT ACTCATGAGA CCAGTGCCCA TGAAGGTCAG
ACTGAGGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGATGGGCGG AAGCCATTTC CAATTAACCA TGGTGAAACT
AGTGATGAAA CTTTATTAGA GGATGCCATA GAAGTTTGCA AGAAGTTTAT GGAGCGCGAC
CCTGATGAAC TAAGATTTAA TGCGATTGCT CTTTCTGCAG CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001257881.1
CDS258..842
Misc Feature(1)159..161(+)
Misc Feature(2)258..830(+)
Misc Feature(3)414..701(+)
Misc Feature(4)414..656(+)
Exon (1)1..191
Gene:UCHL3
Gene Synonym:
Exon (2)192..203
Gene:UCHL3
Gene Synonym:
Exon (3)204..332
Gene:UCHL3
Gene Synonym:
Exon (4)333..489
Gene:UCHL3
Gene Synonym:
Exon (5)490..575
Gene:UCHL3
Gene Synonym:
Exon (6)576..623
Gene:UCHL3
Gene Synonym:
Exon (7)624..699
Gene:UCHL3
Gene Synonym:
Exon (8)700..758
Gene:UCHL3
Gene Synonym:
Exon (9)759..1050
Gene:UCHL3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001270952
Organism Homo sapiens (human)
Definition Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001270952

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGATCCTG AACTCCTTAG CATGGTACCA AGACCAGTCT GTGCAGTCTT ACTTCTCTTT 
CCTATTACAG AAAAGTATGA AGTATTCAGA ACAGAAGAGG AAGAAAAAAT AAAATCTCAG
GGACAAGATG TTACATCATC AGTATATTTC ATGAAGCAAA CAATCAGCAA TGCCTGTGGA
ACAATTGGAC TGATTCATGC TATTGCAAAC AATAAAGACA AGATGCACTT TGAATCTGGA
TCAACCTTGA AAAAATTCCT GGAGGAATCT GTGTCAATGA GCCCTGAAGA ACGAGCCAGA
TACCTGGAGA ACTATGATGC CATCCGAGTT ACTCATGAGA CCAGTGCCCA TGAAGGTCAG
ACTGAGGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGATGGGCGG AAGCCATTTC CAATTAACCA TGGTGAAACT
AGTGATGAAA CTTTATTAGA GGATGCCATA GAAGTTTGCA AGAAGTTTAT GGAGCGCGAC
CCTGATGAAC TAAGATTTAA TGCGATTGCT CTTTCTGCAG CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56052
Clone ID Related Accession (Same CDS sequence) NM_006002
Accession Version NM_006002.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 693bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA820150.1, BC018125.2 and AA234624.1. On Aug 18, 2012 this sequence version replaced gi:37059734. Transcript Variant: This variant (2) contains an alternate exon in the 5' coding region and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI602995.1, BC018125.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGGCACCA
AGTATAGATG AGAAAGTAGA TCTTCATTTT ATTGCATTAG TTCATGTAGA TGGGCATCTC
TATGAATTAG ATGGGCGGAA GCCATTTCCA ATTAACCATG GTGAAACTAG TGATGAAACT
TTATTAGAGG ATGCCATAGA AGTTTGCAAG AAGTTTATGG AGCGCGACCC TGATGAACTA
AGATTTAATG CGATTGCTCT TTCTGCAGCA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005993.1
CDS72..764
Misc Feature(1)87..752(+)
Misc Feature(2)93..710(+)
Misc Feature(3)93..578(+)
Misc Feature(4)93..110(+)
Misc Feature(5)294..296(+)
Misc Feature(6)336..623(+)
Misc Feature(7)459..461(+)
Misc Feature(8)525..548(+)
Misc Feature(9)621..623(+)
Misc Feature(10)726..743(+)
Exon (1)1..113
Gene:UCHL3
Gene Synonym:
Exon (2)114..125
Gene:UCHL3
Gene Synonym:
Exon (3)126..254
Gene:UCHL3
Gene Synonym:
Exon (4)255..411
Gene:UCHL3
Gene Synonym:
Exon (5)412..497
Gene:UCHL3
Gene Synonym:
Exon (6)498..545
Gene:UCHL3
Gene Synonym:
Exon (7)546..621
Gene:UCHL3
Gene Synonym:
Exon (8)622..680
Gene:UCHL3
Gene Synonym:
Exon (9)681..972
Gene:UCHL3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006002
Organism Homo sapiens (human)
Definition Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006002

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGGCACCA
AGTATAGATG AGAAAGTAGA TCTTCATTTT ATTGCATTAG TTCATGTAGA TGGGCATCTC
TATGAATTAG ATGGGCGGAA GCCATTTCCA ATTAACCATG GTGAAACTAG TGATGAAACT
TTATTAGAGG ATGCCATAGA AGTTTGCAAG AAGTTTATGG AGCGCGACCC TGATGAACTA
AGATTTAATG CGATTGCTCT TTCTGCAGCA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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