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KDM6A cDNA ORF clone, Homo sapiens (human)

Gene Symbol KDM6A
Entrez Gene ID 7403
Full Name lysine (K)-specific demethylase 6A
Synonyms KABUK2, UTX, bA386N14.2
General protein information
Preferred Names
lysine-specific demethylase 6A
Names
lysine-specific demethylase 6A
histone demethylase UTX
ubiquitously-transcribed TPR gene on the X chromosome
ubiquitously-transcribed TPR protein on the X chromosome
ubiquitously transcribed tetratricopeptide repeat protein X-linked
bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX))
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp11.2

Summary This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014]. lac of sum
Disorder MIM:

300128


mRNA and Protein(s)

mRNA Protein Name
XM_011543957 XP_011542259 lysine-specific demethylase 6A isoform X1
XM_011543958 XP_011542260 lysine-specific demethylase 6A isoform X2
XM_011543959 XP_011542261 lysine-specific demethylase 6A isoform X3
XM_011543960 XP_011542262 lysine-specific demethylase 6A isoform X4
XM_011543961 XP_011542263 lysine-specific demethylase 6A isoform X5
XM_011543962 XP_011542264 lysine-specific demethylase 6A isoform X6
XM_011543963 XP_011542265 lysine-specific demethylase 6A isoform X7
XM_011543964 XP_011542266 lysine-specific demethylase 6A isoform X8
XM_011543965 XP_011542267 lysine-specific demethylase 6A isoform X9
XM_011543966 XP_011542268 lysine-specific demethylase 6A isoform X10
XM_011543967 XP_011542269 lysine-specific demethylase 6A isoform X11
XM_005272656 XP_005272713 lysine-specific demethylase 6A isoform X12
XM_011543968 XP_011542270 lysine-specific demethylase 6A isoform X13
XM_011543969 XP_011542271 lysine-specific demethylase 6A isoform X14
XM_011543970 XP_011542272 lysine-specific demethylase 6A isoform X15
XM_011543971 XP_011542273 lysine-specific demethylase 6A isoform X16
XM_011543972 XP_011542274 lysine-specific demethylase 6A isoform X17
XM_011543973 XP_011542275 lysine-specific demethylase 6A isoform X18
XM_005272659 XP_005272716 lysine-specific demethylase 6A isoform X19
XM_011543974 XP_011542276 lysine-specific demethylase 6A isoform X20
XM_011543975 XP_011542277 lysine-specific demethylase 6A isoform X21
XM_011543976 XP_011542278 lysine-specific demethylase 6A isoform X22
NM_001291415 NP_001278344 lysine-specific demethylase 6A isoform 1
NM_001291416 NP_001278345 lysine-specific demethylase 6A isoform 2
NM_021140 NP_066963 lysine-specific demethylase 6A isoform 3
NM_001291417 NP_001278346 lysine-specific demethylase 6A isoform 4
NM_001291418 NP_001278347 lysine-specific demethylase 6A isoform 5
NM_001291421 NP_001278350 lysine-specific demethylase 6A isoform 6



Homo sapiens (human) KDM6A NP_066963.2
Pan troglodytes (chimpanzee) KDM6A XP_003954022.1
Macaca mulatta (Rhesus monkey) KDM6A XP_002806253.1
Canis lupus familiaris (dog) KDM6A NP_001184114.1
Bos taurus (cattle) KDM6A NP_001193504.1
Mus musculus (house mouse) Kdm6a NP_033509.1
Rattus norvegicus (Norway rat) Kdm6a XP_006256701.1
Gallus gallus (chicken) KDM6A XP_416762.4
Danio rerio (zebrafish) kdm6a XP_697746.2
Drosophila melanogaster (fruit fly) Utx NP_001188773.1
Caenorhabditis elegans utx-1 NP_509450.1
Xenopus (Silurana) tropicalis (western clawed frog) kdm6a NP_001123807.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KDM6A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KDM6A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu61248 XM_011543957 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $811.30
$1159.00
OHu61249 XM_011543958 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61250 XM_011543959 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61251 XM_011543960 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61252 XM_011543961 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61253 XM_011543962 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61254 XM_011543963 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61255 XM_011543964 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61256 XM_011543965 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61257 XM_011543966 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61258 XM_011543967 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu54022 XM_005272656 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61259 XM_011543968 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61260 XM_011543969 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61261 XM_011543970 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61262 XM_011543971 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X17, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61263 XM_011543972 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X18, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61264 XM_011543973 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X19, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu54024 XM_005272659 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X20, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61265 XM_011543974 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X21, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu61266 XM_011543975 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X22, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu61267 XM_011543976 PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X23, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu24601 NM_001291415 Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu24588 NM_001291416 Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu24583
NM_021140 Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$274.50-$384.30
$549.00
OHu24587 NM_001291417 Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu24605 NM_001291418 Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu24600 NM_001291421 Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu61248
Clone ID Related Accession (Same CDS sequence) XM_011543957
Accession Version XM_011543957.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG
ACACCACAGA AATTACAGCA TTTGGAACAG CTCCGCGCAA ATAGAAATAA TTTAAATCCA
GCACAGAAAC TGATGCTGGA ACAGCTGGAA AGTCAGTTTG TCTTAATGCA ACAACACCAA
ATGAGACCAA CAGGAGTTGC ACAGGTACGA TCTACTGGAA TTCCTAATGG GCCAACAGCT
GACTCATCAC TGCCTACAAA CTCAGTCTCT GGCCAGCAGC CACAGCTTGC TCTGACCAGA
GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT
GGACCCTTTT CTGCAGGCCA TGTTCCCTGT AGCACATCAA GAACGCTGGG AAGTACAGAC
ACTATTTTGA TAGGCAATAA TCATATAACA GGAAGTGGAA GTAATGGAAA CGTGCCTTAC
CTGCAGCGAA ACGCACTCAC TCTACCTCAT AACCGCACAA ACCTGACCAG CAGCGCAGAG
GAGCCGTGGA AAAACCAACT ATCTAACTCC ACTCAGGGGC TTCACAAAGG TCAGAGTTCA
CATTCGGCAG GTCCTAATGG TGAACGACCT CTCTCTTCCA CTGGGCCTTC CCAGCATCTC
CAGGCAGCTG GCTCTGGTAT TCAGAATCAG AACGGACATC CCACCCTGCC TAGCAATTCA
GTAACACAGG GGGCTGCTCT CAATCACCTC TCCTCTCACA CTGCTACCTC AGGTGGACAA
CAAGGCATTA CCTTAACCAA AGAGAGCAAG CCTTCAGGAA ACATATTGAC GGTGCCTGAA
ACAAGCAGGC ACACTGGAGA GACACCTAAC AGCACTGCCA GTGTCGAGGG ACTTCCTAAT
CATGTCCATC AGATGACGGC AGATGCTGTT TGCAGTCCTA GCCATGGAGA TTCTAAGTCA
CCAGGTTTAC TAAGTTCAGA CAATCCTCAG CTCTCTGCCT TGTTGATGGG AAAAGCCAAT
AACAATGTGG GTACTGGAAC CTGTGACAAA GTCAATAACA TCCACCCAGC TGTTCATACA
AAGACTGATA ACTCTGTTGC CTCTTCACCA TCTTCAGCCA TTTCAACAGC AACACCTTCT
CCAAAATCCA CTGAGCAGAC AACCACAAAC AGTGTTACCA GCCTTAACAG CCCTCACAGT
GGGCTACACA CAATTAATGG AGAAGGGATG GAAGAATCTC AGAGCCCCAT GAAAACAGAT
CTGCTTCTGG TTAACCACAA ACCTAGTCCA CAGATCATAC CATCAATGTC TGTGTCCATA
TACCCCAGCT CAGCAGAAGT TCTGAAGGCA TGCAGGAATC TAGGTAAAAA TGGCTTATCT
AACAGTAGCA TTTTGTTGGA TAAATGTCCA CCTCCAAGAC CACCATCTTC ACCATACCCT
CCCTTGCCAA AGGACAAGTT GAATCCACCT ACACCTAGTA TTTACTTGGA AAATAAACGT
GATGCTTTCT TTCCTCCATT ACATCAATTT TGTACAAATC CGAACAACCC TGTTACAGTA
ATACGTGGCC TTGCTGGAGC TCTTAAGTTA GACCTGGGAC TTTTCTCTAC TAAAACTTTG
GTGGAAGCTA ACAATGAACA TATGGTAGAA GTGAGGACAC AGTTGTTGCA GCCAGCAGAT
GAAAACTGGG ATCCCACTGG AACAAAGAAA ATCTGGCATT GTGAAAGTAA TAGATCTCAT
ACTACAATTG CTAAATATGC ACAGTACCAG GCCTCCTCAT TCCAGGAATC ATTGAGAGAA
GAAAATGAAA AAAGAAGTCA TCATAAAGAC CACTCAGATA GTGAATCTAC ATCGTCAGAT
AATTCTGGGA GGAGGAGGAA AGGACCCTTT AAAACCATAA AGTTTGGGAC CAATATTGAC
CTATCTGATG ACAAAAAGTG GAAGTTGCAG CTACATGAGC TGACTAAACT TCCTGCTTTT
GTGCGTGTCG TATCAGCAGG AAATCTTCTA AGCCATGTTG GTCATACCAT ATTGGGCATG
AACACAGTTC AACTATACAT GAAAGTTCCA GGGAGCAGAA CACCAGGTCA TCAGGAAAAT
AACAACTTCT GTTCAGTTAA CATAAATATT GGCCCAGGTG ACTGTGAATG GTTTGTTGTT
CCTGAAGGTT ACTGGGGTGT TCTGAATGAC TTCTGTGAAA AAAATAATTT GAATTTCCTA
ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT CTTTATGAAG CAAATGTTCC AGTGTATAGG
TTTATTCAGC GACCTGGAGA TTTGGTCTGG ATAAATGCAG GCACTGTTCA TTGGGTTCAG
GCTATTGGCT GGTGCAACAA CATTGCTTGG AATGTTGGTC CACTTACAGC CTGCCAGTAT
AAATTGGCAG TGGAACGGTA CGAATGGAAC AAATTGCAAA GTGTGAAGTC AATAGTACCC
ATGGTTCATC TTTCCTGGAA TATGGCACGA AATATCAAGG TCTCAGATCC AAAGCTTTTT
GAAATGATTA AGATATTTTC ATTGTCTCCG AATTTTAGAG CTGAAAAGTG CCTTAGAGAT
CATCTAGTTC AACCTCTCCG TTCAAATGGA GAACCTGAGC CACTAAGATT CACAGGAGAG
TATTGTCTTC TAAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA
GGAAAAGAGA TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCTCATTA CTGTAGCATT
TGTGAAGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC ACGAAAGACC
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGCG GAAACTTGGA AAACTTTGTG
GTGCTAGAAC AGTACAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT
CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542259.1
CDS378..4904
Misc Feature(1)654..740(+)
Misc Feature(2)657..971(+)
Misc Feature(3)810..1631(+)
Misc Feature(4)819..911(+)
Misc Feature(5)822..923(+)
Misc Feature(6)924..1016(+)
Misc Feature(7)1047..1133(+)
Misc Feature(8)1050..1322(+)
Misc Feature(9)1146..1271(+)
Misc Feature(10)1248..1343(+)
Misc Feature(11)1284..1370(+)
Misc Feature(12)1287..1526(+)
Misc Feature(13)1287..1370(+)
Misc Feature(14)1383..1475(+)
Misc Feature(15)1386..1487(+)
Misc Feature(16)1488..1568(+)
Misc Feature(17)3885..4079(+)
Misc Feature(18)3987..4313(+)
Translation

Target ORF information:

RefSeq Version XM_011543957
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543957

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG
ACACCACAGA AATTACAGCA TTTGGAACAG CTCCGCGCAA ATAGAAATAA TTTAAATCCA
GCACAGAAAC TGATGCTGGA ACAGCTGGAA AGTCAGTTTG TCTTAATGCA ACAACACCAA
ATGAGACCAA CAGGAGTTGC ACAGGTACGA TCTACTGGAA TTCCTAATGG GCCAACAGCT
GACTCATCAC TGCCTACAAA CTCAGTCTCT GGCCAGCAGC CACAGCTTGC TCTGACCAGA
GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT
GGACCCTTTT CTGCAGGCCA TGTTCCCTGT AGCACATCAA GAACGCTGGG AAGTACAGAC
ACTATTTTGA TAGGCAATAA TCATATAACA GGAAGTGGAA GTAATGGAAA CGTGCCTTAC
CTGCAGCGAA ACGCACTCAC TCTACCTCAT AACCGCACAA ACCTGACCAG CAGCGCAGAG
GAGCCGTGGA AAAACCAACT ATCTAACTCC ACTCAGGGGC TTCACAAAGG TCAGAGTTCA
CATTCGGCAG GTCCTAATGG TGAACGACCT CTCTCTTCCA CTGGGCCTTC CCAGCATCTC
CAGGCAGCTG GCTCTGGTAT TCAGAATCAG AACGGACATC CCACCCTGCC TAGCAATTCA
GTAACACAGG GGGCTGCTCT CAATCACCTC TCCTCTCACA CTGCTACCTC AGGTGGACAA
CAAGGCATTA CCTTAACCAA AGAGAGCAAG CCTTCAGGAA ACATATTGAC GGTGCCTGAA
ACAAGCAGGC ACACTGGAGA GACACCTAAC AGCACTGCCA GTGTCGAGGG ACTTCCTAAT
CATGTCCATC AGATGACGGC AGATGCTGTT TGCAGTCCTA GCCATGGAGA TTCTAAGTCA
CCAGGTTTAC TAAGTTCAGA CAATCCTCAG CTCTCTGCCT TGTTGATGGG AAAAGCCAAT
AACAATGTGG GTACTGGAAC CTGTGACAAA GTCAATAACA TCCACCCAGC TGTTCATACA
AAGACTGATA ACTCTGTTGC CTCTTCACCA TCTTCAGCCA TTTCAACAGC AACACCTTCT
CCAAAATCCA CTGAGCAGAC AACCACAAAC AGTGTTACCA GCCTTAACAG CCCTCACAGT
GGGCTACACA CAATTAATGG AGAAGGGATG GAAGAATCTC AGAGCCCCAT GAAAACAGAT
CTGCTTCTGG TTAACCACAA ACCTAGTCCA CAGATCATAC CATCAATGTC TGTGTCCATA
TACCCCAGCT CAGCAGAAGT TCTGAAGGCA TGCAGGAATC TAGGTAAAAA TGGCTTATCT
AACAGTAGCA TTTTGTTGGA TAAATGTCCA CCTCCAAGAC CACCATCTTC ACCATACCCT
CCCTTGCCAA AGGACAAGTT GAATCCACCT ACACCTAGTA TTTACTTGGA AAATAAACGT
GATGCTTTCT TTCCTCCATT ACATCAATTT TGTACAAATC CGAACAACCC TGTTACAGTA
ATACGTGGCC TTGCTGGAGC TCTTAAGTTA GACCTGGGAC TTTTCTCTAC TAAAACTTTG
GTGGAAGCTA ACAATGAACA TATGGTAGAA GTGAGGACAC AGTTGTTGCA GCCAGCAGAT
GAAAACTGGG ATCCCACTGG AACAAAGAAA ATCTGGCATT GTGAAAGTAA TAGATCTCAT
ACTACAATTG CTAAATATGC ACAGTACCAG GCCTCCTCAT TCCAGGAATC ATTGAGAGAA
GAAAATGAAA AAAGAAGTCA TCATAAAGAC CACTCAGATA GTGAATCTAC ATCGTCAGAT
AATTCTGGGA GGAGGAGGAA AGGACCCTTT AAAACCATAA AGTTTGGGAC CAATATTGAC
CTATCTGATG ACAAAAAGTG GAAGTTGCAG CTACATGAGC TGACTAAACT TCCTGCTTTT
GTGCGTGTCG TATCAGCAGG AAATCTTCTA AGCCATGTTG GTCATACCAT ATTGGGCATG
AACACAGTTC AACTATACAT GAAAGTTCCA GGGAGCAGAA CACCAGGTCA TCAGGAAAAT
AACAACTTCT GTTCAGTTAA CATAAATATT GGCCCAGGTG ACTGTGAATG GTTTGTTGTT
CCTGAAGGTT ACTGGGGTGT TCTGAATGAC TTCTGTGAAA AAAATAATTT GAATTTCCTA
ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT CTTTATGAAG CAAATGTTCC AGTGTATAGG
TTTATTCAGC GACCTGGAGA TTTGGTCTGG ATAAATGCAG GCACTGTTCA TTGGGTTCAG
GCTATTGGCT GGTGCAACAA CATTGCTTGG AATGTTGGTC CACTTACAGC CTGCCAGTAT
AAATTGGCAG TGGAACGGTA CGAATGGAAC AAATTGCAAA GTGTGAAGTC AATAGTACCC
ATGGTTCATC TTTCCTGGAA TATGGCACGA AATATCAAGG TCTCAGATCC AAAGCTTTTT
GAAATGATTA AGATATTTTC ATTGTCTCCG AATTTTAGAG CTGAAAAGTG CCTTAGAGAT
CATCTAGTTC AACCTCTCCG TTCAAATGGA GAACCTGAGC CACTAAGATT CACAGGAGAG
TATTGTCTTC TAAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA
GGAAAAGAGA TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCTCATTA CTGTAGCATT
TGTGAAGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC ACGAAAGACC
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGCG GAAACTTGGA AAACTTTGTG
GTGCTAGAAC AGTACAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT
CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61249
Clone ID Related Accession (Same CDS sequence) XM_011543958
Accession Version XM_011543958.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT
TTGACACCAC AGAAATTACA GCATTTGGAA CAGCTCCGCG CAAATAGAAA TAATTTAAAT
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC
CAAATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT
TTTGAAATGA TTAAGATATT TTCATTGTCT CCGAATTTTA GAGCTGAAAA GTGCCTTAGA
GATCATCTAG TTCAACCTCT CCGTTCAAAT GGAGAACCTG AGCCACTAAG ATTCACAGGA
GAGTATTGTC TTCTAAGAAC TCTGAAGCAA TGTCAGACAT TGAGGGAAGC TCTCATTGCT
GCAGGAAAAG AGATTATATG GCATGGGCGG ACAAAAGAAG AACCAGCTCA TTACTGTAGC
ATTTGTGAAG TGGAGGTTTT TGATCTGCTT TTTGTCACTA ATGAGAGTAA TTCACGAAAG
ACCTACATAG TACATTGCCA AGATTGTGCA CGAAAAACAA GCGGAAACTT GGAAAACTTT
GTGGTGCTAG AACAGTACAA AATGGAGGAC CTGATGCAAG TCTATGACCA ATTTACATTA
GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542260.1
CDS378..4847
Misc Feature(1)654..740(+)
Misc Feature(2)657..914(+)
Misc Feature(3)693..1574(+)
Misc Feature(4)762..854(+)
Misc Feature(5)765..866(+)
Misc Feature(6)867..959(+)
Misc Feature(7)990..1076(+)
Misc Feature(8)993..1265(+)
Misc Feature(9)1089..1214(+)
Misc Feature(10)1191..1286(+)
Misc Feature(11)1227..1313(+)
Misc Feature(12)1230..1469(+)
Misc Feature(13)1230..1313(+)
Misc Feature(14)1326..1418(+)
Misc Feature(15)1329..1430(+)
Misc Feature(16)1431..1511(+)
Misc Feature(17)3828..4022(+)
Misc Feature(18)3930..4256(+)
Translation

Target ORF information:

RefSeq Version XM_011543958
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543958

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT
TTGACACCAC AGAAATTACA GCATTTGGAA CAGCTCCGCG CAAATAGAAA TAATTTAAAT
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC
CAAATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT
TTTGAAATGA TTAAGATATT TTCATTGTCT CCGAATTTTA GAGCTGAAAA GTGCCTTAGA
GATCATCTAG TTCAACCTCT CCGTTCAAAT GGAGAACCTG AGCCACTAAG ATTCACAGGA
GAGTATTGTC TTCTAAGAAC TCTGAAGCAA TGTCAGACAT TGAGGGAAGC TCTCATTGCT
GCAGGAAAAG AGATTATATG GCATGGGCGG ACAAAAGAAG AACCAGCTCA TTACTGTAGC
ATTTGTGAAG TGGAGGTTTT TGATCTGCTT TTTGTCACTA ATGAGAGTAA TTCACGAAAG
ACCTACATAG TACATTGCCA AGATTGTGCA CGAAAAACAA GCGGAAACTT GGAAAACTTT
GTGGTGCTAG AACAGTACAA AATGGAGGAC CTGATGCAAG TCTATGACCA ATTTACATTA
GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61250
Clone ID Related Accession (Same CDS sequence) XM_011543959
Accession Version XM_011543959.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4425bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG
ACACCACAGA AATTACAGAT GAGACCAACA GGAGTTGCAC AGGTACGATC TACTGGAATT
CCTAATGGGC CAACAGCTGA CTCATCACTG CCTACAAACT CAGTCTCTGG CCAGCAGCCA
CAGCTTGCTC TGACCAGAGT GCCTAGCGTC TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT
GGGCAGCCTT TGGCCAATGG ACCCTTTTCT GCAGGCCATG TTCCCTGTAG CACATCAAGA
ACGCTGGGAA GTACAGACAC TATTTTGATA GGCAATAATC ATATAACAGG AAGTGGAAGT
AATGGAAACG TGCCTTACCT GCAGCGAAAC GCACTCACTC TACCTCATAA CCGCACAAAC
CTGACCAGCA GCGCAGAGGA GCCGTGGAAA AACCAACTAT CTAACTCCAC TCAGGGGCTT
CACAAAGGTC AGAGTTCACA TTCGGCAGGT CCTAATGGTG AACGACCTCT CTCTTCCACT
GGGCCTTCCC AGCATCTCCA GGCAGCTGGC TCTGGTATTC AGAATCAGAA CGGACATCCC
ACCCTGCCTA GCAATTCAGT AACACAGGGG GCTGCTCTCA ATCACCTCTC CTCTCACACT
GCTACCTCAG GTGGACAACA AGGCATTACC TTAACCAAAG AGAGCAAGCC TTCAGGAAAC
ATATTGACGG TGCCTGAAAC AAGCAGGCAC ACTGGAGAGA CACCTAACAG CACTGCCAGT
GTCGAGGGAC TTCCTAATCA TGTCCATCAG ATGACGGCAG ATGCTGTTTG CAGTCCTAGC
CATGGAGATT CTAAGTCACC AGGTTTACTA AGTTCAGACA ATCCTCAGCT CTCTGCCTTG
TTGATGGGAA AAGCCAATAA CAATGTGGGT ACTGGAACCT GTGACAAAGT CAATAACATC
CACCCAGCTG TTCATACAAA GACTGATAAC TCTGTTGCCT CTTCACCATC TTCAGCCATT
TCAACAGCAA CACCTTCTCC AAAATCCACT GAGCAGACAA CCACAAACAG TGTTACCAGC
CTTAACAGCC CTCACAGTGG GCTACACACA ATTAATGGAG AAGGGATGGA AGAATCTCAG
AGCCCCATGA AAACAGATCT GCTTCTGGTT AACCACAAAC CTAGTCCACA GATCATACCA
TCAATGTCTG TGTCCATATA CCCCAGCTCA GCAGAAGTTC TGAAGGCATG CAGGAATCTA
GGTAAAAATG GCTTATCTAA CAGTAGCATT TTGTTGGATA AATGTCCACC TCCAAGACCA
CCATCTTCAC CATACCCTCC CTTGCCAAAG GACAAGTTGA ATCCACCTAC ACCTAGTATT
TACTTGGAAA ATAAACGTGA TGCTTTCTTT CCTCCATTAC ATCAATTTTG TACAAATCCG
AACAACCCTG TTACAGTAAT ACGTGGCCTT GCTGGAGCTC TTAAGTTAGA CCTGGGACTT
TTCTCTACTA AAACTTTGGT GGAAGCTAAC AATGAACATA TGGTAGAAGT GAGGACACAG
TTGTTGCAGC CAGCAGATGA AAACTGGGAT CCCACTGGAA CAAAGAAAAT CTGGCATTGT
GAAAGTAATA GATCTCATAC TACAATTGCT AAATATGCAC AGTACCAGGC CTCCTCATTC
CAGGAATCAT TGAGAGAAGA AAATGAAAAA AGAAGTCATC ATAAAGACCA CTCAGATAGT
GAATCTACAT CGTCAGATAA TTCTGGGAGG AGGAGGAAAG GACCCTTTAA AACCATAAAG
TTTGGGACCA ATATTGACCT ATCTGATGAC AAAAAGTGGA AGTTGCAGCT ACATGAGCTG
ACTAAACTTC CTGCTTTTGT GCGTGTCGTA TCAGCAGGAA ATCTTCTAAG CCATGTTGGT
CATACCATAT TGGGCATGAA CACAGTTCAA CTATACATGA AAGTTCCAGG GAGCAGAACA
CCAGGTCATC AGGAAAATAA CAACTTCTGT TCAGTTAACA TAAATATTGG CCCAGGTGAC
TGTGAATGGT TTGTTGTTCC TGAAGGTTAC TGGGGTGTTC TGAATGACTT CTGTGAAAAA
AATAATTTGA ATTTCCTAAT GGGTTCTTGG TGGCCCAATC TTGAAGATCT TTATGAAGCA
AATGTTCCAG TGTATAGGTT TATTCAGCGA CCTGGAGATT TGGTCTGGAT AAATGCAGGC
ACTGTTCATT GGGTTCAGGC TATTGGCTGG TGCAACAACA TTGCTTGGAA TGTTGGTCCA
CTTACAGCCT GCCAGTATAA ATTGGCAGTG GAACGGTACG AATGGAACAA ATTGCAAAGT
GTGAAGTCAA TAGTACCCAT GGTTCATCTT TCCTGGAATA TGGCACGAAA TATCAAGGTC
TCAGATCCAA AGCTTTTTGA AATGATTAAG ATATTTTCAT TGTCTCCGAA TTTTAGAGCT
GAAAAGTGCC TTAGAGATCA TCTAGTTCAA CCTCTCCGTT CAAATGGAGA ACCTGAGCCA
CTAAGATTCA CAGGAGAGTA TTGTCTTCTA AGAACTCTGA AGCAATGTCA GACATTGAGG
GAAGCTCTCA TTGCTGCAGG AAAAGAGATT ATATGGCATG GGCGGACAAA AGAAGAACCA
GCTCATTACT GTAGCATTTG TGAAGTGGAG GTTTTTGATC TGCTTTTTGT CACTAATGAG
AGTAATTCAC GAAAGACCTA CATAGTACAT TGCCAAGATT GTGCACGAAA AACAAGCGGA
AACTTGGAAA ACTTTGTGGT GCTAGAACAG TACAAAATGG AGGACCTGAT GCAAGTCTAT
GACCAATTTA CATTAGCTCC TCCATTACCA TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542261.1
CDS378..4802
Misc Feature(1)654..740(+)
Misc Feature(2)657..971(+)
Misc Feature(3)810..1631(+)
Misc Feature(4)819..911(+)
Misc Feature(5)822..923(+)
Misc Feature(6)924..1016(+)
Misc Feature(7)1047..1133(+)
Misc Feature(8)1050..1322(+)
Misc Feature(9)1146..1271(+)
Misc Feature(10)1248..1343(+)
Misc Feature(11)1284..1370(+)
Misc Feature(12)1287..1526(+)
Misc Feature(13)1287..1370(+)
Misc Feature(14)1383..1475(+)
Misc Feature(15)1386..1487(+)
Misc Feature(16)1488..1568(+)
Misc Feature(17)3783..3977(+)
Misc Feature(18)3885..4211(+)
Translation

Target ORF information:

RefSeq Version XM_011543959
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543959

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG
ACACCACAGA AATTACAGAT GAGACCAACA GGAGTTGCAC AGGTACGATC TACTGGAATT
CCTAATGGGC CAACAGCTGA CTCATCACTG CCTACAAACT CAGTCTCTGG CCAGCAGCCA
CAGCTTGCTC TGACCAGAGT GCCTAGCGTC TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT
GGGCAGCCTT TGGCCAATGG ACCCTTTTCT GCAGGCCATG TTCCCTGTAG CACATCAAGA
ACGCTGGGAA GTACAGACAC TATTTTGATA GGCAATAATC ATATAACAGG AAGTGGAAGT
AATGGAAACG TGCCTTACCT GCAGCGAAAC GCACTCACTC TACCTCATAA CCGCACAAAC
CTGACCAGCA GCGCAGAGGA GCCGTGGAAA AACCAACTAT CTAACTCCAC TCAGGGGCTT
CACAAAGGTC AGAGTTCACA TTCGGCAGGT CCTAATGGTG AACGACCTCT CTCTTCCACT
GGGCCTTCCC AGCATCTCCA GGCAGCTGGC TCTGGTATTC AGAATCAGAA CGGACATCCC
ACCCTGCCTA GCAATTCAGT AACACAGGGG GCTGCTCTCA ATCACCTCTC CTCTCACACT
GCTACCTCAG GTGGACAACA AGGCATTACC TTAACCAAAG AGAGCAAGCC TTCAGGAAAC
ATATTGACGG TGCCTGAAAC AAGCAGGCAC ACTGGAGAGA CACCTAACAG CACTGCCAGT
GTCGAGGGAC TTCCTAATCA TGTCCATCAG ATGACGGCAG ATGCTGTTTG CAGTCCTAGC
CATGGAGATT CTAAGTCACC AGGTTTACTA AGTTCAGACA ATCCTCAGCT CTCTGCCTTG
TTGATGGGAA AAGCCAATAA CAATGTGGGT ACTGGAACCT GTGACAAAGT CAATAACATC
CACCCAGCTG TTCATACAAA GACTGATAAC TCTGTTGCCT CTTCACCATC TTCAGCCATT
TCAACAGCAA CACCTTCTCC AAAATCCACT GAGCAGACAA CCACAAACAG TGTTACCAGC
CTTAACAGCC CTCACAGTGG GCTACACACA ATTAATGGAG AAGGGATGGA AGAATCTCAG
AGCCCCATGA AAACAGATCT GCTTCTGGTT AACCACAAAC CTAGTCCACA GATCATACCA
TCAATGTCTG TGTCCATATA CCCCAGCTCA GCAGAAGTTC TGAAGGCATG CAGGAATCTA
GGTAAAAATG GCTTATCTAA CAGTAGCATT TTGTTGGATA AATGTCCACC TCCAAGACCA
CCATCTTCAC CATACCCTCC CTTGCCAAAG GACAAGTTGA ATCCACCTAC ACCTAGTATT
TACTTGGAAA ATAAACGTGA TGCTTTCTTT CCTCCATTAC ATCAATTTTG TACAAATCCG
AACAACCCTG TTACAGTAAT ACGTGGCCTT GCTGGAGCTC TTAAGTTAGA CCTGGGACTT
TTCTCTACTA AAACTTTGGT GGAAGCTAAC AATGAACATA TGGTAGAAGT GAGGACACAG
TTGTTGCAGC CAGCAGATGA AAACTGGGAT CCCACTGGAA CAAAGAAAAT CTGGCATTGT
GAAAGTAATA GATCTCATAC TACAATTGCT AAATATGCAC AGTACCAGGC CTCCTCATTC
CAGGAATCAT TGAGAGAAGA AAATGAAAAA AGAAGTCATC ATAAAGACCA CTCAGATAGT
GAATCTACAT CGTCAGATAA TTCTGGGAGG AGGAGGAAAG GACCCTTTAA AACCATAAAG
TTTGGGACCA ATATTGACCT ATCTGATGAC AAAAAGTGGA AGTTGCAGCT ACATGAGCTG
ACTAAACTTC CTGCTTTTGT GCGTGTCGTA TCAGCAGGAA ATCTTCTAAG CCATGTTGGT
CATACCATAT TGGGCATGAA CACAGTTCAA CTATACATGA AAGTTCCAGG GAGCAGAACA
CCAGGTCATC AGGAAAATAA CAACTTCTGT TCAGTTAACA TAAATATTGG CCCAGGTGAC
TGTGAATGGT TTGTTGTTCC TGAAGGTTAC TGGGGTGTTC TGAATGACTT CTGTGAAAAA
AATAATTTGA ATTTCCTAAT GGGTTCTTGG TGGCCCAATC TTGAAGATCT TTATGAAGCA
AATGTTCCAG TGTATAGGTT TATTCAGCGA CCTGGAGATT TGGTCTGGAT AAATGCAGGC
ACTGTTCATT GGGTTCAGGC TATTGGCTGG TGCAACAACA TTGCTTGGAA TGTTGGTCCA
CTTACAGCCT GCCAGTATAA ATTGGCAGTG GAACGGTACG AATGGAACAA ATTGCAAAGT
GTGAAGTCAA TAGTACCCAT GGTTCATCTT TCCTGGAATA TGGCACGAAA TATCAAGGTC
TCAGATCCAA AGCTTTTTGA AATGATTAAG ATATTTTCAT TGTCTCCGAA TTTTAGAGCT
GAAAAGTGCC TTAGAGATCA TCTAGTTCAA CCTCTCCGTT CAAATGGAGA ACCTGAGCCA
CTAAGATTCA CAGGAGAGTA TTGTCTTCTA AGAACTCTGA AGCAATGTCA GACATTGAGG
GAAGCTCTCA TTGCTGCAGG AAAAGAGATT ATATGGCATG GGCGGACAAA AGAAGAACCA
GCTCATTACT GTAGCATTTG TGAAGTGGAG GTTTTTGATC TGCTTTTTGT CACTAATGAG
AGTAATTCAC GAAAGACCTA CATAGTACAT TGCCAAGATT GTGCACGAAA AACAAGCGGA
AACTTGGAAA ACTTTGTGGT GCTAGAACAG TACAAAATGG AGGACCTGAT GCAAGTCTAT
GACCAATTTA CATTAGCTCC TCCATTACCA TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61251
Clone ID Related Accession (Same CDS sequence) XM_011543960
Accession Version XM_011543960.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4419bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG
ACACCACAGA AATTACAGCA TTTGGAACAG CTCCGCGCAA ATAGAAATAA TTTAAATCCA
GCACAGAAAC TGATGCTGGA ACAGCTGGAA AGTCAGTTTG TCTTAATGCA ACAACACCAA
ATGAGACCAA CAGGAGTTGC ACAGGTACGA TCTACTGGAA TTCCTAATGG GCCAACAGCT
GACTCATCAC TGCCTACAAA CTCAGTCTCT GGCCAGCAGC CACAGCTTGC TCTGACCAGA
GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT
GGACCCTTTT CTGCAGGCCA TGTTCCCTGT AGCACATCAA GAACGCTGGG AAGTACAGAC
ACTATTTTGA TAGGCAATAA TCATATAACA GGAAGTGGAA GTAATGGAAA CGTGCCTTAC
CTGCAGCGAA ACGCACTCAC TCTACCTCAT AACCGCACAA ACCTGACCAG CAGCGCAGAG
GAGCCGTGGA AAAACCAACT ATCTAACTCC ACTCAGGGGC TTCACAAAGG TCAGAGTTCA
CATTCGGCAG GTCCTAATGG TGAACGACCT CTCTCTTCCA CTGGGCCTTC CCAGCATCTC
CAGGCAGCTG GCTCTGGTAT TCAGAATCAG AACGGACATC CCACCCTGCC TAGCAATTCA
GTAACACAGG GGGCTGCTCT CAATCACCTC TCCTCTCACA CTGCTACCTC AGGTGGACAA
CAAGGCATTA CCTTAACCAA AGAGAGCAAG CCTTCAGGAA ACATATTGAC GGTGCCTGAA
ACAAGCAGGC ACACTGGAGA GACACCTAAC AGCACTGCCA GTGTCGAGGG ACTTCCTAAT
CATGTCCATC AGATGACGGC AGATGCTGTT TGCAGTCCTA GCCATGGAGA TTCTAAGTCA
CCAGGTTTAC TAAGTTCAGA CAATCCTCAG CTCTCTGCCT TGTTGATGGG AAAAGCCAAT
AACAATGTGG GTACTGGAAC CTGTGACAAA GTCAATAACA TCCACCCAGC TGTTCATACA
AAGACTGATA ACTCTGTTGC CTCTTCACCA TCTTCAGCCA TTTCAACAGC AACACCTTCT
CCAAAATCCA CTGAGCAGAC AACCACAAAC AGTGTTACCA GCCTTAACAG CCCTCACAGT
GGGCTACACA CAATTAATGG AGAAGGGATG GAAGAATCTC AGAGCCCCAT GAAAACAGAT
CTGCTTCTGG TTAACCACAA ACCTAGTCCA CAGATCATAC CATCAATGTC TGTGTCCATA
TACCCCAGCT CAGCAGAAGT TCTGAAGGCA TGCAGGAATC TAGGTAAAAA TGGCTTATCT
AACAGTAGCA TTTTGTTGGA TAAATGTCCA CCTCCAAGAC CACCATCTTC ACCATACCCT
CCCTTGCCAA AGGACAAGTT GAATCCACCT ACACCTAGTA TTTACTTGGA AAATAAACGT
GATGCTTTCT TTCCTCCATT ACATCAATTT TGTACAAATC CGAACAACCC TGTTACAGTA
ATACGTGGCC TTGCTGGAGC TCTTAAGTTA GACCTGGGAC TTTTCTCTAC TAAAACTTTG
GTGGAAGCTA ACAATGAACA TATGGTAGAA GTGAGGACAC AGTTGTTGCA GCCAGCAGAT
GAAAACTGGG ATCCCACTGG AACAAAGAAA ATCTGGCATT GTGAAAGTAA TAGATCTCAT
ACTACAATTG CTAAATATGC ACAGTACCAG GCCTCCTCAT TCCAGGAATC ATTGAGAGAA
GAAAATGAAA AAAGAAGTCA TCATAAAGAC CACTCAGATA GTGAATCTAC ATCGTCAGAT
AATTCTGGGA GGAGGAGGAA AGGACCCTTT AAAACCATAA AGTTTGGGAC CAATATTGAC
CTATCTGATG ACAAAAAGTG GAAGTTGCAG CTACATGAGC TGACTAAACT TCCTGCTTTT
GTGCGTGTCG TATCAGCAGG AAATCTTCTA AGCCATGTTG GTCATACCAT ATTGGGCATG
AACACAGTTC AACTATACAT GAAAGTTCCA GGGAGCAGAA CACCAGGTCA TCAGGAAAAT
AACAACTTCT GTTCAGTTAA CATAAATATT GGCCCAGGTG ACTGTGAATG GTTTGTTGTT
CCTGAAGGTT ACTGGGGTGT TCTGAATGAC TTCTGTGAAA AAAATAATTT GAATTTCCTA
ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT CTTTATGAAG CAAATGTTCC AGTGTATAGG
TTTATTCAGC GACCTGGAGA TTTGGTCTGG ATAAATGCAG GCACTGTTCA TTGGGTTCAG
GCTATTGGCT GGTGCAACAA CATTGCTTGG AATGTTGGTC CACTTACAGC CTGCCAGTAT
AAATTGGCAG TGGAACGGTA CGAATGGAAC AAATTGCAAA GTGTGAAGTC AATAGTACCC
ATGGTTCATC TTTCCTGGAA TATGGCACGA AATATCAAGG TCTCAGATCC AAAGCTTTTT
GAAATGATTA AGTATTGTCT TCTAAGAACT CTGAAGCAAT GTCAGACATT GAGGGAAGCT
CTCATTGCTG CAGGAAAAGA GATTATATGG CATGGGCGGA CAAAAGAAGA ACCAGCTCAT
TACTGTAGCA TTTGTGAAGT GGAGGTTTTT GATCTGCTTT TTGTCACTAA TGAGAGTAAT
TCACGAAAGA CCTACATAGT ACATTGCCAA GATTGTGCAC GAAAAACAAG CGGAAACTTG
GAAAACTTTG TGGTGCTAGA ACAGTACAAA ATGGAGGACC TGATGCAAGT CTATGACCAA
TTTACATTAG CTCCTCCATT ACCATCCGCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542262.1
CDS378..4796
Misc Feature(1)654..740(+)
Misc Feature(2)657..971(+)
Misc Feature(3)810..1631(+)
Misc Feature(4)819..911(+)
Misc Feature(5)822..923(+)
Misc Feature(6)924..1016(+)
Misc Feature(7)1047..1133(+)
Misc Feature(8)1050..1322(+)
Misc Feature(9)1146..1271(+)
Misc Feature(10)1248..1343(+)
Misc Feature(11)1284..1370(+)
Misc Feature(12)1287..1526(+)
Misc Feature(13)1287..1370(+)
Misc Feature(14)1383..1475(+)
Misc Feature(15)1386..1487(+)
Misc Feature(16)1488..1568(+)
Misc Feature(17)3885..4079(+)
Misc Feature(18)3987..4313(+)
Translation

Target ORF information:

RefSeq Version XM_011543960
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543960

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG
ACACCACAGA AATTACAGCA TTTGGAACAG CTCCGCGCAA ATAGAAATAA TTTAAATCCA
GCACAGAAAC TGATGCTGGA ACAGCTGGAA AGTCAGTTTG TCTTAATGCA ACAACACCAA
ATGAGACCAA CAGGAGTTGC ACAGGTACGA TCTACTGGAA TTCCTAATGG GCCAACAGCT
GACTCATCAC TGCCTACAAA CTCAGTCTCT GGCCAGCAGC CACAGCTTGC TCTGACCAGA
GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT
GGACCCTTTT CTGCAGGCCA TGTTCCCTGT AGCACATCAA GAACGCTGGG AAGTACAGAC
ACTATTTTGA TAGGCAATAA TCATATAACA GGAAGTGGAA GTAATGGAAA CGTGCCTTAC
CTGCAGCGAA ACGCACTCAC TCTACCTCAT AACCGCACAA ACCTGACCAG CAGCGCAGAG
GAGCCGTGGA AAAACCAACT ATCTAACTCC ACTCAGGGGC TTCACAAAGG TCAGAGTTCA
CATTCGGCAG GTCCTAATGG TGAACGACCT CTCTCTTCCA CTGGGCCTTC CCAGCATCTC
CAGGCAGCTG GCTCTGGTAT TCAGAATCAG AACGGACATC CCACCCTGCC TAGCAATTCA
GTAACACAGG GGGCTGCTCT CAATCACCTC TCCTCTCACA CTGCTACCTC AGGTGGACAA
CAAGGCATTA CCTTAACCAA AGAGAGCAAG CCTTCAGGAA ACATATTGAC GGTGCCTGAA
ACAAGCAGGC ACACTGGAGA GACACCTAAC AGCACTGCCA GTGTCGAGGG ACTTCCTAAT
CATGTCCATC AGATGACGGC AGATGCTGTT TGCAGTCCTA GCCATGGAGA TTCTAAGTCA
CCAGGTTTAC TAAGTTCAGA CAATCCTCAG CTCTCTGCCT TGTTGATGGG AAAAGCCAAT
AACAATGTGG GTACTGGAAC CTGTGACAAA GTCAATAACA TCCACCCAGC TGTTCATACA
AAGACTGATA ACTCTGTTGC CTCTTCACCA TCTTCAGCCA TTTCAACAGC AACACCTTCT
CCAAAATCCA CTGAGCAGAC AACCACAAAC AGTGTTACCA GCCTTAACAG CCCTCACAGT
GGGCTACACA CAATTAATGG AGAAGGGATG GAAGAATCTC AGAGCCCCAT GAAAACAGAT
CTGCTTCTGG TTAACCACAA ACCTAGTCCA CAGATCATAC CATCAATGTC TGTGTCCATA
TACCCCAGCT CAGCAGAAGT TCTGAAGGCA TGCAGGAATC TAGGTAAAAA TGGCTTATCT
AACAGTAGCA TTTTGTTGGA TAAATGTCCA CCTCCAAGAC CACCATCTTC ACCATACCCT
CCCTTGCCAA AGGACAAGTT GAATCCACCT ACACCTAGTA TTTACTTGGA AAATAAACGT
GATGCTTTCT TTCCTCCATT ACATCAATTT TGTACAAATC CGAACAACCC TGTTACAGTA
ATACGTGGCC TTGCTGGAGC TCTTAAGTTA GACCTGGGAC TTTTCTCTAC TAAAACTTTG
GTGGAAGCTA ACAATGAACA TATGGTAGAA GTGAGGACAC AGTTGTTGCA GCCAGCAGAT
GAAAACTGGG ATCCCACTGG AACAAAGAAA ATCTGGCATT GTGAAAGTAA TAGATCTCAT
ACTACAATTG CTAAATATGC ACAGTACCAG GCCTCCTCAT TCCAGGAATC ATTGAGAGAA
GAAAATGAAA AAAGAAGTCA TCATAAAGAC CACTCAGATA GTGAATCTAC ATCGTCAGAT
AATTCTGGGA GGAGGAGGAA AGGACCCTTT AAAACCATAA AGTTTGGGAC CAATATTGAC
CTATCTGATG ACAAAAAGTG GAAGTTGCAG CTACATGAGC TGACTAAACT TCCTGCTTTT
GTGCGTGTCG TATCAGCAGG AAATCTTCTA AGCCATGTTG GTCATACCAT ATTGGGCATG
AACACAGTTC AACTATACAT GAAAGTTCCA GGGAGCAGAA CACCAGGTCA TCAGGAAAAT
AACAACTTCT GTTCAGTTAA CATAAATATT GGCCCAGGTG ACTGTGAATG GTTTGTTGTT
CCTGAAGGTT ACTGGGGTGT TCTGAATGAC TTCTGTGAAA AAAATAATTT GAATTTCCTA
ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT CTTTATGAAG CAAATGTTCC AGTGTATAGG
TTTATTCAGC GACCTGGAGA TTTGGTCTGG ATAAATGCAG GCACTGTTCA TTGGGTTCAG
GCTATTGGCT GGTGCAACAA CATTGCTTGG AATGTTGGTC CACTTACAGC CTGCCAGTAT
AAATTGGCAG TGGAACGGTA CGAATGGAAC AAATTGCAAA GTGTGAAGTC AATAGTACCC
ATGGTTCATC TTTCCTGGAA TATGGCACGA AATATCAAGG TCTCAGATCC AAAGCTTTTT
GAAATGATTA AGTATTGTCT TCTAAGAACT CTGAAGCAAT GTCAGACATT GAGGGAAGCT
CTCATTGCTG CAGGAAAAGA GATTATATGG CATGGGCGGA CAAAAGAAGA ACCAGCTCAT
TACTGTAGCA TTTGTGAAGT GGAGGTTTTT GATCTGCTTT TTGTCACTAA TGAGAGTAAT
TCACGAAAGA CCTACATAGT ACATTGCCAA GATTGTGCAC GAAAAACAAG CGGAAACTTG
GAAAACTTTG TGGTGCTAGA ACAGTACAAA ATGGAGGACC TGATGCAAGT CTATGACCAA
TTTACATTAG CTCCTCCATT ACCATCCGCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61252
Clone ID Related Accession (Same CDS sequence) XM_011543961
Accession Version XM_011543961.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA
CAGCATTTGG AACAGCTCCG CGCAAATAGA AATAATTTAA ATCCAGCACA GAAACTGATG
CTGGAACAGC TGGAAAGTCA GTTTGTCTTA ATGCAACAAC ACCAAATGAG ACCAACAGGA
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGATA
TTTTCATTGT CTCCGAATTT TAGAGCTGAA AAGTGCCTTA GAGATCATCT AGTTCAACCT
CTCCGTTCAA ATGGAGAACC TGAGCCACTA AGATTCACAG GAGAGTATTG TCTTCTAAGA
ACTCTGAAGC AATGTCAGAC ATTGAGGGAA GCTCTCATTG CTGCAGGAAA AGAGATTATA
TGGCATGGGC GGACAAAAGA AGAACCAGCT CATTACTGTA GCATTTGTGA AGTGGAGGTT
TTTGATCTGC TTTTTGTCAC TAATGAGAGT AATTCACGAA AGACCTACAT AGTACATTGC
CAAGATTGTG CACGAAAAAC AAGCGGAAAC TTGGAAAACT TTGTGGTGCT AGAACAGTAC
AAAATGGAGG ACCTGATGCA AGTCTATGAC CAATTTACAT TAGCTCCTCC ATTACCATCC
GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542263.1
CDS378..4769
Misc Feature(1)654..740(+)
Misc Feature(2)657..971(+)
Misc Feature(3)810..1637(+)
Misc Feature(4)819..911(+)
Misc Feature(5)822..923(+)
Misc Feature(6)924..1016(+)
Misc Feature(7)1047..1133(+)
Misc Feature(8)1050..1322(+)
Misc Feature(9)1146..1271(+)
Misc Feature(10)1248..1343(+)
Misc Feature(11)1284..1370(+)
Misc Feature(12)1287..1526(+)
Misc Feature(13)1287..1370(+)
Misc Feature(14)1383..1475(+)
Misc Feature(15)1386..1487(+)
Misc Feature(16)1488..1568(+)
Misc Feature(17)3750..3944(+)
Misc Feature(18)3852..4178(+)
Translation

Target ORF information:

RefSeq Version XM_011543961
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543961

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA
CAGCATTTGG AACAGCTCCG CGCAAATAGA AATAATTTAA ATCCAGCACA GAAACTGATG
CTGGAACAGC TGGAAAGTCA GTTTGTCTTA ATGCAACAAC ACCAAATGAG ACCAACAGGA
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGATA
TTTTCATTGT CTCCGAATTT TAGAGCTGAA AAGTGCCTTA GAGATCATCT AGTTCAACCT
CTCCGTTCAA ATGGAGAACC TGAGCCACTA AGATTCACAG GAGAGTATTG TCTTCTAAGA
ACTCTGAAGC AATGTCAGAC ATTGAGGGAA GCTCTCATTG CTGCAGGAAA AGAGATTATA
TGGCATGGGC GGACAAAAGA AGAACCAGCT CATTACTGTA GCATTTGTGA AGTGGAGGTT
TTTGATCTGC TTTTTGTCAC TAATGAGAGT AATTCACGAA AGACCTACAT AGTACATTGC
CAAGATTGTG CACGAAAAAC AAGCGGAAAC TTGGAAAACT TTGTGGTGCT AGAACAGTAC
AAAATGGAGG ACCTGATGCA AGTCTATGAC CAATTTACAT TAGCTCCTCC ATTACCATCC
GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61253
Clone ID Related Accession (Same CDS sequence) XM_011543962
Accession Version XM_011543962.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGAATA CTTCTGACAA TTGGAGTGGT GGACATGCTG TGTCACATCC TCCAGTACAG
CAACAAGCTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGCATTTGGA ACAGCTCCGC
GCAAATAGAA ATAATTTAAA TCCAGCACAG AAACTGATGC TGGAACAGCT GGAAAGTCAG
TTTGTCTTAA TGCAACAACA CCAAATGAGA CCAACAGGAG TTGCACAGGT ACGATCTACT
GGAATTCCTA ATGGGCCAAC AGCTGACTCA TCACTGCCTA CAAACTCAGT CTCTGGCCAG
CAGCCACAGC TTGCTCTGAC CAGAGTGCCT AGCGTCTCTC AGCCTGGAGT CCGTCCTGCC
TGCCCTGGGC AGCCTTTGGC CAATGGACCC TTTTCTGCAG GCCATGTTCC CTGTAGCACA
TCAAGAACGC TGGGAAGTAC AGACACTATT TTGATAGGCA ATAATCATAT AACAGGAAGT
GGAAGTAATG GAAACGTGCC TTACCTGCAG CGAAACGCAC TCACTCTACC TCATAACCGC
ACAAACCTGA CCAGCAGCGC AGAGGAGCCG TGGAAAAACC AACTATCTAA CTCCACTCAG
GGGCTTCACA AAGGTCAGAG TTCACATTCG GCAGGTCCTA ATGGTGAACG ACCTCTCTCT
TCCACTGGGC CTTCCCAGCA TCTCCAGGCA GCTGGCTCTG GTATTCAGAA TCAGAACGGA
CATCCCACCC TGCCTAGCAA TTCAGTAACA CAGGGGGCTG CTCTCAATCA CCTCTCCTCT
CACACTGCTA CCTCAGGTGG ACAACAAGGC ATTACCTTAA CCAAAGAGAG CAAGCCTTCA
GGAAACATAT TGACGGTGCC TGAAACAAGC AGGCACACTG GAGAGACACC TAACAGCACT
GCCAGTGTCG AGGGACTTCC TAATCATGTC CATCAGATGA CGGCAGATGC TGTTTGCAGT
CCTAGCCATG GAGATTCTAA GTCACCAGGT TTACTAAGTT CAGACAATCC TCAGCTCTCT
GCCTTGTTGA TGGGAAAAGC CAATAACAAT GTGGGTACTG GAACCTGTGA CAAAGTCAAT
AACATCCACC CAGCTGTTCA TACAAAGACT GATAACTCTG TTGCCTCTTC ACCATCTTCA
GCCATTTCAA CAGCAACACC TTCTCCAAAA TCCACTGAGC AGACAACCAC AAACAGTGTT
ACCAGCCTTA ACAGCCCTCA CAGTGGGCTA CACACAATTA ATGGAGAAGG GATGGAAGAA
TCTCAGAGCC CCATGAAAAC AGATCTGCTT CTGGTTAACC ACAAACCTAG TCCACAGATC
ATACCATCAA TGTCTGTGTC CATATACCCC AGCTCAGCAG AAGTTCTGAA GGCATGCAGG
AATCTAGGTA AAAATGGCTT ATCTAACAGT AGCATTTTGT TGGATAAATG TCCACCTCCA
AGACCACCAT CTTCACCATA CCCTCCCTTG CCAAAGGACA AGTTGAATCC ACCTACACCT
AGTATTTACT TGGAAAATAA ACGTGATGCT TTCTTTCCTC CATTACATCA ATTTTGTACA
AATCCGAACA ACCCTGTTAC AGTAATACGT GGCCTTGCTG GAGCTCTTAA GTTAGACCTG
GGACTTTTCT CTACTAAAAC TTTGGTGGAA GCTAACAATG AACATATGGT AGAAGTGAGG
ACACAGTTGT TGCAGCCAGC AGATGAAAAC TGGGATCCCA CTGGAACAAA GAAAATCTGG
CATTGTGAAA GTAATAGATC TCATACTACA ATTGCTAAAT ATGCACAGTA CCAGGCCTCC
TCATTCCAGG AATCATTGAG AGAAGAAAAT GAAAAAAGAA GTCATCATAA AGACCACTCA
GATAGTGAAT CTACATCGTC AGATAATTCT GGGAGGAGGA GGAAAGGACC CTTTAAAACC
ATAAAGTTTG GGACCAATAT TGACCTATCT GATGACAAAA AGTGGAAGTT GCAGCTACAT
GAGCTGACTA AACTTCCTGC TTTTGTGCGT GTCGTATCAG CAGGAAATCT TCTAAGCCAT
GTTGGTCATA CCATATTGGG CATGAACACA GTTCAACTAT ACATGAAAGT TCCAGGGAGC
AGAACACCAG GTCATCAGGA AAATAACAAC TTCTGTTCAG TTAACATAAA TATTGGCCCA
GGTGACTGTG AATGGTTTGT TGTTCCTGAA GGTTACTGGG GTGTTCTGAA TGACTTCTGT
GAAAAAAATA ATTTGAATTT CCTAATGGGT TCTTGGTGGC CCAATCTTGA AGATCTTTAT
GAAGCAAATG TTCCAGTGTA TAGGTTTATT CAGCGACCTG GAGATTTGGT CTGGATAAAT
GCAGGCACTG TTCATTGGGT TCAGGCTATT GGCTGGTGCA ACAACATTGC TTGGAATGTT
GGTCCACTTA CAGCCTGCCA GTATAAATTG GCAGTGGAAC GGTACGAATG GAACAAATTG
CAAAGTGTGA AGTCAATAGT ACCCATGGTT CATCTTTCCT GGAATATGGC ACGAAATATC
AAGGTCTCAG ATCCAAAGCT TTTTGAAATG ATTAAGATAT TTTCATTGTC TCCGAATTTT
AGAGCTGAAA AGTGCCTTAG AGATCATCTA GTTCAACCTC TCCGTTCAAA TGGAGAACCT
GAGCCACTAA GATTCACAGG AGAGTATTGT CTTCTAAGAA CTCTGAAGCA ATGTCAGACA
TTGAGGGAAG CTCTCATTGC TGCAGGAAAA GAGATTATAT GGCATGGGCG GACAAAAGAA
GAACCAGCTC ATTACTGTAG CATTTGTGAA GTGGAGGTTT TTGATCTGCT TTTTGTCACT
AATGAGAGTA ATTCACGAAA GACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA
AGCGGAAACT TGGAAAACTT TGTGGTGCTA GAACAGTACA AAATGGAGGA CCTGATGCAA
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCG CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542264.1
CDS378..4748
Misc Feature(1)654..740(+)
Misc Feature(2)657..971(+)
Misc Feature(3)810..1631(+)
Misc Feature(4)819..911(+)
Misc Feature(5)822..923(+)
Misc Feature(6)924..1016(+)
Misc Feature(7)1047..1133(+)
Misc Feature(8)1050..1322(+)
Misc Feature(9)1146..1271(+)
Misc Feature(10)1248..1343(+)
Misc Feature(11)1284..1370(+)
Misc Feature(12)1287..1526(+)
Misc Feature(13)1287..1370(+)
Misc Feature(14)1383..1475(+)
Misc Feature(15)1386..1487(+)
Misc Feature(16)1488..1568(+)
Misc Feature(17)3729..3923(+)
Misc Feature(18)3831..4157(+)
Translation

Target ORF information:

RefSeq Version XM_011543962
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543962

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGAATA CTTCTGACAA TTGGAGTGGT GGACATGCTG TGTCACATCC TCCAGTACAG
CAACAAGCTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGCATTTGGA ACAGCTCCGC
GCAAATAGAA ATAATTTAAA TCCAGCACAG AAACTGATGC TGGAACAGCT GGAAAGTCAG
TTTGTCTTAA TGCAACAACA CCAAATGAGA CCAACAGGAG TTGCACAGGT ACGATCTACT
GGAATTCCTA ATGGGCCAAC AGCTGACTCA TCACTGCCTA CAAACTCAGT CTCTGGCCAG
CAGCCACAGC TTGCTCTGAC CAGAGTGCCT AGCGTCTCTC AGCCTGGAGT CCGTCCTGCC
TGCCCTGGGC AGCCTTTGGC CAATGGACCC TTTTCTGCAG GCCATGTTCC CTGTAGCACA
TCAAGAACGC TGGGAAGTAC AGACACTATT TTGATAGGCA ATAATCATAT AACAGGAAGT
GGAAGTAATG GAAACGTGCC TTACCTGCAG CGAAACGCAC TCACTCTACC TCATAACCGC
ACAAACCTGA CCAGCAGCGC AGAGGAGCCG TGGAAAAACC AACTATCTAA CTCCACTCAG
GGGCTTCACA AAGGTCAGAG TTCACATTCG GCAGGTCCTA ATGGTGAACG ACCTCTCTCT
TCCACTGGGC CTTCCCAGCA TCTCCAGGCA GCTGGCTCTG GTATTCAGAA TCAGAACGGA
CATCCCACCC TGCCTAGCAA TTCAGTAACA CAGGGGGCTG CTCTCAATCA CCTCTCCTCT
CACACTGCTA CCTCAGGTGG ACAACAAGGC ATTACCTTAA CCAAAGAGAG CAAGCCTTCA
GGAAACATAT TGACGGTGCC TGAAACAAGC AGGCACACTG GAGAGACACC TAACAGCACT
GCCAGTGTCG AGGGACTTCC TAATCATGTC CATCAGATGA CGGCAGATGC TGTTTGCAGT
CCTAGCCATG GAGATTCTAA GTCACCAGGT TTACTAAGTT CAGACAATCC TCAGCTCTCT
GCCTTGTTGA TGGGAAAAGC CAATAACAAT GTGGGTACTG GAACCTGTGA CAAAGTCAAT
AACATCCACC CAGCTGTTCA TACAAAGACT GATAACTCTG TTGCCTCTTC ACCATCTTCA
GCCATTTCAA CAGCAACACC TTCTCCAAAA TCCACTGAGC AGACAACCAC AAACAGTGTT
ACCAGCCTTA ACAGCCCTCA CAGTGGGCTA CACACAATTA ATGGAGAAGG GATGGAAGAA
TCTCAGAGCC CCATGAAAAC AGATCTGCTT CTGGTTAACC ACAAACCTAG TCCACAGATC
ATACCATCAA TGTCTGTGTC CATATACCCC AGCTCAGCAG AAGTTCTGAA GGCATGCAGG
AATCTAGGTA AAAATGGCTT ATCTAACAGT AGCATTTTGT TGGATAAATG TCCACCTCCA
AGACCACCAT CTTCACCATA CCCTCCCTTG CCAAAGGACA AGTTGAATCC ACCTACACCT
AGTATTTACT TGGAAAATAA ACGTGATGCT TTCTTTCCTC CATTACATCA ATTTTGTACA
AATCCGAACA ACCCTGTTAC AGTAATACGT GGCCTTGCTG GAGCTCTTAA GTTAGACCTG
GGACTTTTCT CTACTAAAAC TTTGGTGGAA GCTAACAATG AACATATGGT AGAAGTGAGG
ACACAGTTGT TGCAGCCAGC AGATGAAAAC TGGGATCCCA CTGGAACAAA GAAAATCTGG
CATTGTGAAA GTAATAGATC TCATACTACA ATTGCTAAAT ATGCACAGTA CCAGGCCTCC
TCATTCCAGG AATCATTGAG AGAAGAAAAT GAAAAAAGAA GTCATCATAA AGACCACTCA
GATAGTGAAT CTACATCGTC AGATAATTCT GGGAGGAGGA GGAAAGGACC CTTTAAAACC
ATAAAGTTTG GGACCAATAT TGACCTATCT GATGACAAAA AGTGGAAGTT GCAGCTACAT
GAGCTGACTA AACTTCCTGC TTTTGTGCGT GTCGTATCAG CAGGAAATCT TCTAAGCCAT
GTTGGTCATA CCATATTGGG CATGAACACA GTTCAACTAT ACATGAAAGT TCCAGGGAGC
AGAACACCAG GTCATCAGGA AAATAACAAC TTCTGTTCAG TTAACATAAA TATTGGCCCA
GGTGACTGTG AATGGTTTGT TGTTCCTGAA GGTTACTGGG GTGTTCTGAA TGACTTCTGT
GAAAAAAATA ATTTGAATTT CCTAATGGGT TCTTGGTGGC CCAATCTTGA AGATCTTTAT
GAAGCAAATG TTCCAGTGTA TAGGTTTATT CAGCGACCTG GAGATTTGGT CTGGATAAAT
GCAGGCACTG TTCATTGGGT TCAGGCTATT GGCTGGTGCA ACAACATTGC TTGGAATGTT
GGTCCACTTA CAGCCTGCCA GTATAAATTG GCAGTGGAAC GGTACGAATG GAACAAATTG
CAAAGTGTGA AGTCAATAGT ACCCATGGTT CATCTTTCCT GGAATATGGC ACGAAATATC
AAGGTCTCAG ATCCAAAGCT TTTTGAAATG ATTAAGATAT TTTCATTGTC TCCGAATTTT
AGAGCTGAAA AGTGCCTTAG AGATCATCTA GTTCAACCTC TCCGTTCAAA TGGAGAACCT
GAGCCACTAA GATTCACAGG AGAGTATTGT CTTCTAAGAA CTCTGAAGCA ATGTCAGACA
TTGAGGGAAG CTCTCATTGC TGCAGGAAAA GAGATTATAT GGCATGGGCG GACAAAAGAA
GAACCAGCTC ATTACTGTAG CATTTGTGAA GTGGAGGTTT TTGATCTGCT TTTTGTCACT
AATGAGAGTA ATTCACGAAA GACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA
AGCGGAAACT TGGAAAACTT TGTGGTGCTA GAACAGTACA AAATGGAGGA CCTGATGCAA
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCG CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61254
Clone ID Related Accession (Same CDS sequence) XM_011543963
Accession Version XM_011543963.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4335bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA
TTACAGCATT TGGAACAGCT CCGCGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACCAACA
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC
TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA
GGCAATAATC ATATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTCGGCAGGT
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC
TCTGGTATTC AGAATCAGAA CGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ATATTGACGG TGCCTGAAAC AAGCAGGCAC
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG
ATGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAC
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCAACAGCAA CACCTTCTCC AAAATCCACT
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTACACACA
ATTAATGGAG AAGGGATGGA AGAATCTCAG AGCCCCATGA AAACAGATCT GCTTCTGGTT
AACCACAAAC CTAGTCCACA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT
TTGTTGGATA AATGTCCACC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG
GACAAGTTGA ATCCACCTAC ACCTAGTATT TACTTGGAAA ATAAACGTGA TGCTTTCTTT
CCTCCATTAC ATCAATTTTG TACAAATCCG AACAACCCTG TTACAGTAAT ACGTGGCCTT
GCTGGAGCTC TTAAGTTAGA CCTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA AAACTGGGAT
CCCACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT
AAATATGCAC AGTACCAGGC CTCCTCATTC CAGGAATCAT TGAGAGAAGA AAATGAAAAA
AGAAGTCATC ATAAAGACCA CTCAGATAGT GAATCTACAT CGTCAGATAA TTCTGGGAGG
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ATATTGACCT ATCTGATGAC
AAAAAGTGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA
TCAGCAGGAA ATCTTCTAAG CCATGTTGGT CATACCATAT TGGGCATGAA CACAGTTCAA
CTATACATGA AAGTTCCAGG GAGCAGAACA CCAGGTCATC AGGAAAATAA CAACTTCTGT
TCAGTTAACA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGTTAC
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG
TGGCCCAATC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG
ATATTTTCAT TGTCTCCGAA TTTTAGAGCT GAAAAGTGCC TTAGAGATCA TCTAGTTCAA
CCTCTCCGTT CAAATGGAGA ACCTGAGCCA CTAAGATTCA CAGGAGAGTA TTGTCTTCTA
AGAACTCTGA AGCAATGTCA GACATTGAGG GAAGCTCTCA TTGCTGCAGG AAAAGAGATT
ATATGGCATG GGCGGACAAA AGAAGAACCA GCTCATTACT GTAGCATTTG TGAAGTGGAG
GTTTTTGATC TGCTTTTTGT CACTAATGAG AGTAATTCAC GAAAGACCTA CATAGTACAT
TGCCAAGATT GTGCACGAAA AACAAGCGGA AACTTGGAAA ACTTTGTGGT GCTAGAACAG
TACAAAATGG AGGACCTGAT GCAAGTCTAT GACCAATTTA CATTAGCTCC TCCATTACCA
TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542265.1
CDS378..4712
Misc Feature(1)654..740(+)
Misc Feature(2)657..914(+)
Misc Feature(3)693..1580(+)
Misc Feature(4)762..854(+)
Misc Feature(5)765..866(+)
Misc Feature(6)867..959(+)
Misc Feature(7)990..1076(+)
Misc Feature(8)993..1265(+)
Misc Feature(9)1089..1214(+)
Misc Feature(10)1191..1286(+)
Misc Feature(11)1227..1313(+)
Misc Feature(12)1230..1469(+)
Misc Feature(13)1230..1313(+)
Misc Feature(14)1326..1418(+)
Misc Feature(15)1329..1430(+)
Misc Feature(16)1431..1511(+)
Misc Feature(17)3693..3887(+)
Misc Feature(18)3795..4121(+)
Translation

Target ORF information:

RefSeq Version XM_011543963
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543963

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA
TTACAGCATT TGGAACAGCT CCGCGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACCAACA
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC
TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA
GGCAATAATC ATATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTCGGCAGGT
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC
TCTGGTATTC AGAATCAGAA CGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ATATTGACGG TGCCTGAAAC AAGCAGGCAC
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG
ATGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAC
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCAACAGCAA CACCTTCTCC AAAATCCACT
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTACACACA
ATTAATGGAG AAGGGATGGA AGAATCTCAG AGCCCCATGA AAACAGATCT GCTTCTGGTT
AACCACAAAC CTAGTCCACA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT
TTGTTGGATA AATGTCCACC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG
GACAAGTTGA ATCCACCTAC ACCTAGTATT TACTTGGAAA ATAAACGTGA TGCTTTCTTT
CCTCCATTAC ATCAATTTTG TACAAATCCG AACAACCCTG TTACAGTAAT ACGTGGCCTT
GCTGGAGCTC TTAAGTTAGA CCTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA AAACTGGGAT
CCCACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT
AAATATGCAC AGTACCAGGC CTCCTCATTC CAGGAATCAT TGAGAGAAGA AAATGAAAAA
AGAAGTCATC ATAAAGACCA CTCAGATAGT GAATCTACAT CGTCAGATAA TTCTGGGAGG
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ATATTGACCT ATCTGATGAC
AAAAAGTGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA
TCAGCAGGAA ATCTTCTAAG CCATGTTGGT CATACCATAT TGGGCATGAA CACAGTTCAA
CTATACATGA AAGTTCCAGG GAGCAGAACA CCAGGTCATC AGGAAAATAA CAACTTCTGT
TCAGTTAACA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGTTAC
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG
TGGCCCAATC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG
ATATTTTCAT TGTCTCCGAA TTTTAGAGCT GAAAAGTGCC TTAGAGATCA TCTAGTTCAA
CCTCTCCGTT CAAATGGAGA ACCTGAGCCA CTAAGATTCA CAGGAGAGTA TTGTCTTCTA
AGAACTCTGA AGCAATGTCA GACATTGAGG GAAGCTCTCA TTGCTGCAGG AAAAGAGATT
ATATGGCATG GGCGGACAAA AGAAGAACCA GCTCATTACT GTAGCATTTG TGAAGTGGAG
GTTTTTGATC TGCTTTTTGT CACTAATGAG AGTAATTCAC GAAAGACCTA CATAGTACAT
TGCCAAGATT GTGCACGAAA AACAAGCGGA AACTTGGAAA ACTTTGTGGT GCTAGAACAG
TACAAAATGG AGGACCTGAT GCAAGTCTAT GACCAATTTA CATTAGCTCC TCCATTACCA
TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61255
Clone ID Related Accession (Same CDS sequence) XM_011543964
Accession Version XM_011543964.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4314bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGA TATTTTCATT GTCTCCGAAT
TTTAGAGCTG AAAAGTGCCT TAGAGATCAT CTAGTTCAAC CTCTCCGTTC AAATGGAGAA
CCTGAGCCAC TAAGATTCAC AGGAGAGTAT TGTCTTCTAA GAACTCTGAA GCAATGTCAG
ACATTGAGGG AAGCTCTCAT TGCTGCAGGA AAAGAGATTA TATGGCATGG GCGGACAAAA
GAAGAACCAG CTCATTACTG TAGCATTTGT GAAGTGGAGG TTTTTGATCT GCTTTTTGTC
ACTAATGAGA GTAATTCACG AAAGACCTAC ATAGTACATT GCCAAGATTG TGCACGAAAA
ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG CTAGAACAGT ACAAAATGGA GGACCTGATG
CAAGTCTATG ACCAATTTAC ATTAGCTCCT CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542266.1
CDS378..4691
Misc Feature(1)654..740(+)
Misc Feature(2)657..914(+)
Misc Feature(3)693..1574(+)
Misc Feature(4)762..854(+)
Misc Feature(5)765..866(+)
Misc Feature(6)867..959(+)
Misc Feature(7)990..1076(+)
Misc Feature(8)993..1265(+)
Misc Feature(9)1089..1214(+)
Misc Feature(10)1191..1286(+)
Misc Feature(11)1227..1313(+)
Misc Feature(12)1230..1469(+)
Misc Feature(13)1230..1313(+)
Misc Feature(14)1326..1418(+)
Misc Feature(15)1329..1430(+)
Misc Feature(16)1431..1511(+)
Misc Feature(17)3672..3866(+)
Misc Feature(18)3774..4100(+)
Translation

Target ORF information:

RefSeq Version XM_011543964
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543964

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGA TATTTTCATT GTCTCCGAAT
TTTAGAGCTG AAAAGTGCCT TAGAGATCAT CTAGTTCAAC CTCTCCGTTC AAATGGAGAA
CCTGAGCCAC TAAGATTCAC AGGAGAGTAT TGTCTTCTAA GAACTCTGAA GCAATGTCAG
ACATTGAGGG AAGCTCTCAT TGCTGCAGGA AAAGAGATTA TATGGCATGG GCGGACAAAA
GAAGAACCAG CTCATTACTG TAGCATTTGT GAAGTGGAGG TTTTTGATCT GCTTTTTGTC
ACTAATGAGA GTAATTCACG AAAGACCTAC ATAGTACATT GCCAAGATTG TGCACGAAAA
ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG CTAGAACAGT ACAAAATGGA GGACCTGATG
CAAGTCTATG ACCAATTTAC ATTAGCTCCT CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61256
Clone ID Related Accession (Same CDS sequence) XM_011543965
Accession Version XM_011543965.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA
CAGATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT
TTTGAAATGA TTAAGATATT TTCATTGTCT CCGAATTTTA GAGCTGAAAA GTGCCTTAGA
GATCATCTAG TTCAACCTCT CCGTTCAAAT GGAGAACCTG AGCCACTAAG ATTCACAGGA
GAGTATTGTC TTCTAAGAAC TCTGAAGCAA TGTCAGACAT TGAGGGAAGC TCTCATTGCT
GCAGGAAAAG AGATTATATG GCATGGGCGG ACAAAAGAAG AACCAGCTCA TTACTGTAGC
ATTTGTGAAG TGGAGGTTTT TGATCTGCTT TTTGTCACTA ATGAGAGTAA TTCACGAAAG
ACCTACATAG TACATTGCCA AGATTGTGCA CGAAAAACAA GCGGAAACTT GGAAAACTTT
GTGGTGCTAG AACAGTACAA AATGGAGGAC CTGATGCAAG TCTATGACCA ATTTACATTA
GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542267.1
CDS378..4667
Misc Feature(1)654..740(+)
Misc Feature(2)657..971(+)
Misc Feature(3)810..1637(+)
Misc Feature(4)819..911(+)
Misc Feature(5)822..923(+)
Misc Feature(6)924..1016(+)
Misc Feature(7)1047..1133(+)
Misc Feature(8)1050..1322(+)
Misc Feature(9)1146..1271(+)
Misc Feature(10)1248..1343(+)
Misc Feature(11)1284..1370(+)
Misc Feature(12)1287..1526(+)
Misc Feature(13)1287..1370(+)
Misc Feature(14)1383..1475(+)
Misc Feature(15)1386..1487(+)
Misc Feature(16)1488..1568(+)
Misc Feature(17)3648..3842(+)
Misc Feature(18)3750..4076(+)
Translation

Target ORF information:

RefSeq Version XM_011543965
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543965

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA
CAGATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT
TTTGAAATGA TTAAGATATT TTCATTGTCT CCGAATTTTA GAGCTGAAAA GTGCCTTAGA
GATCATCTAG TTCAACCTCT CCGTTCAAAT GGAGAACCTG AGCCACTAAG ATTCACAGGA
GAGTATTGTC TTCTAAGAAC TCTGAAGCAA TGTCAGACAT TGAGGGAAGC TCTCATTGCT
GCAGGAAAAG AGATTATATG GCATGGGCGG ACAAAAGAAG AACCAGCTCA TTACTGTAGC
ATTTGTGAAG TGGAGGTTTT TGATCTGCTT TTTGTCACTA ATGAGAGTAA TTCACGAAAG
ACCTACATAG TACATTGCCA AGATTGTGCA CGAAAAACAA GCGGAAACTT GGAAAACTTT
GTGGTGCTAG AACAGTACAA AATGGAGGAC CTGATGCAAG TCTATGACCA ATTTACATTA
GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61257
Clone ID Related Accession (Same CDS sequence) XM_011543966
Accession Version XM_011543966.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4269bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGAATA CTTCTGACAA TTGGAGTGGT GGACATGCTG TGTCACATCC TCCAGTACAG
CAACAAGCTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGATGAGACC AACAGGAGTT
GCACAGGTAC GATCTACTGG AATTCCTAAT GGGCCAACAG CTGACTCATC ACTGCCTACA
AACTCAGTCT CTGGCCAGCA GCCACAGCTT GCTCTGACCA GAGTGCCTAG CGTCTCTCAG
CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG CCTTTGGCCA ATGGACCCTT TTCTGCAGGC
CATGTTCCCT GTAGCACATC AAGAACGCTG GGAAGTACAG ACACTATTTT GATAGGCAAT
AATCATATAA CAGGAAGTGG AAGTAATGGA AACGTGCCTT ACCTGCAGCG AAACGCACTC
ACTCTACCTC ATAACCGCAC AAACCTGACC AGCAGCGCAG AGGAGCCGTG GAAAAACCAA
CTATCTAACT CCACTCAGGG GCTTCACAAA GGTCAGAGTT CACATTCGGC AGGTCCTAAT
GGTGAACGAC CTCTCTCTTC CACTGGGCCT TCCCAGCATC TCCAGGCAGC TGGCTCTGGT
ATTCAGAATC AGAACGGACA TCCCACCCTG CCTAGCAATT CAGTAACACA GGGGGCTGCT
CTCAATCACC TCTCCTCTCA CACTGCTACC TCAGGTGGAC AACAAGGCAT TACCTTAACC
AAAGAGAGCA AGCCTTCAGG AAACATATTG ACGGTGCCTG AAACAAGCAG GCACACTGGA
GAGACACCTA ACAGCACTGC CAGTGTCGAG GGACTTCCTA ATCATGTCCA TCAGATGACG
GCAGATGCTG TTTGCAGTCC TAGCCATGGA GATTCTAAGT CACCAGGTTT ACTAAGTTCA
GACAATCCTC AGCTCTCTGC CTTGTTGATG GGAAAAGCCA ATAACAATGT GGGTACTGGA
ACCTGTGACA AAGTCAATAA CATCCACCCA GCTGTTCATA CAAAGACTGA TAACTCTGTT
GCCTCTTCAC CATCTTCAGC CATTTCAACA GCAACACCTT CTCCAAAATC CACTGAGCAG
ACAACCACAA ACAGTGTTAC CAGCCTTAAC AGCCCTCACA GTGGGCTACA CACAATTAAT
GGAGAAGGGA TGGAAGAATC TCAGAGCCCC ATGAAAACAG ATCTGCTTCT GGTTAACCAC
AAACCTAGTC CACAGATCAT ACCATCAATG TCTGTGTCCA TATACCCCAG CTCAGCAGAA
GTTCTGAAGG CATGCAGGAA TCTAGGTAAA AATGGCTTAT CTAACAGTAG CATTTTGTTG
GATAAATGTC CACCTCCAAG ACCACCATCT TCACCATACC CTCCCTTGCC AAAGGACAAG
TTGAATCCAC CTACACCTAG TATTTACTTG GAAAATAAAC GTGATGCTTT CTTTCCTCCA
TTACATCAAT TTTGTACAAA TCCGAACAAC CCTGTTACAG TAATACGTGG CCTTGCTGGA
GCTCTTAAGT TAGACCTGGG ACTTTTCTCT ACTAAAACTT TGGTGGAAGC TAACAATGAA
CATATGGTAG AAGTGAGGAC ACAGTTGTTG CAGCCAGCAG ATGAAAACTG GGATCCCACT
GGAACAAAGA AAATCTGGCA TTGTGAAAGT AATAGATCTC ATACTACAAT TGCTAAATAT
GCACAGTACC AGGCCTCCTC ATTCCAGGAA TCATTGAGAG AAGAAAATGA AAAAAGAAGT
CATCATAAAG ACCACTCAGA TAGTGAATCT ACATCGTCAG ATAATTCTGG GAGGAGGAGG
AAAGGACCCT TTAAAACCAT AAAGTTTGGG ACCAATATTG ACCTATCTGA TGACAAAAAG
TGGAAGTTGC AGCTACATGA GCTGACTAAA CTTCCTGCTT TTGTGCGTGT CGTATCAGCA
GGAAATCTTC TAAGCCATGT TGGTCATACC ATATTGGGCA TGAACACAGT TCAACTATAC
ATGAAAGTTC CAGGGAGCAG AACACCAGGT CATCAGGAAA ATAACAACTT CTGTTCAGTT
AACATAAATA TTGGCCCAGG TGACTGTGAA TGGTTTGTTG TTCCTGAAGG TTACTGGGGT
GTTCTGAATG ACTTCTGTGA AAAAAATAAT TTGAATTTCC TAATGGGTTC TTGGTGGCCC
AATCTTGAAG ATCTTTATGA AGCAAATGTT CCAGTGTATA GGTTTATTCA GCGACCTGGA
GATTTGGTCT GGATAAATGC AGGCACTGTT CATTGGGTTC AGGCTATTGG CTGGTGCAAC
AACATTGCTT GGAATGTTGG TCCACTTACA GCCTGCCAGT ATAAATTGGC AGTGGAACGG
TACGAATGGA ACAAATTGCA AAGTGTGAAG TCAATAGTAC CCATGGTTCA TCTTTCCTGG
AATATGGCAC GAAATATCAA GGTCTCAGAT CCAAAGCTTT TTGAAATGAT TAAGATATTT
TCATTGTCTC CGAATTTTAG AGCTGAAAAG TGCCTTAGAG ATCATCTAGT TCAACCTCTC
CGTTCAAATG GAGAACCTGA GCCACTAAGA TTCACAGGAG AGTATTGTCT TCTAAGAACT
CTGAAGCAAT GTCAGACATT GAGGGAAGCT CTCATTGCTG CAGGAAAAGA GATTATATGG
CATGGGCGGA CAAAAGAAGA ACCAGCTCAT TACTGTAGCA TTTGTGAAGT GGAGGTTTTT
GATCTGCTTT TTGTCACTAA TGAGAGTAAT TCACGAAAGA CCTACATAGT ACATTGCCAA
GATTGTGCAC GAAAAACAAG CGGAAACTTG GAAAACTTTG TGGTGCTAGA ACAGTACAAA
ATGGAGGACC TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCGCC
TCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542268.1
CDS378..4646
Misc Feature(1)654..740(+)
Misc Feature(2)657..971(+)
Misc Feature(3)810..1631(+)
Misc Feature(4)819..911(+)
Misc Feature(5)822..923(+)
Misc Feature(6)924..1016(+)
Misc Feature(7)1047..1133(+)
Misc Feature(8)1050..1322(+)
Misc Feature(9)1146..1271(+)
Misc Feature(10)1248..1343(+)
Misc Feature(11)1284..1370(+)
Misc Feature(12)1287..1526(+)
Misc Feature(13)1287..1370(+)
Misc Feature(14)1383..1475(+)
Misc Feature(15)1386..1487(+)
Misc Feature(16)1488..1568(+)
Misc Feature(17)3627..3821(+)
Misc Feature(18)3729..4055(+)
Translation

Target ORF information:

RefSeq Version XM_011543966
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543966

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGAATA CTTCTGACAA TTGGAGTGGT GGACATGCTG TGTCACATCC TCCAGTACAG
CAACAAGCTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGATGAGACC AACAGGAGTT
GCACAGGTAC GATCTACTGG AATTCCTAAT GGGCCAACAG CTGACTCATC ACTGCCTACA
AACTCAGTCT CTGGCCAGCA GCCACAGCTT GCTCTGACCA GAGTGCCTAG CGTCTCTCAG
CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG CCTTTGGCCA ATGGACCCTT TTCTGCAGGC
CATGTTCCCT GTAGCACATC AAGAACGCTG GGAAGTACAG ACACTATTTT GATAGGCAAT
AATCATATAA CAGGAAGTGG AAGTAATGGA AACGTGCCTT ACCTGCAGCG AAACGCACTC
ACTCTACCTC ATAACCGCAC AAACCTGACC AGCAGCGCAG AGGAGCCGTG GAAAAACCAA
CTATCTAACT CCACTCAGGG GCTTCACAAA GGTCAGAGTT CACATTCGGC AGGTCCTAAT
GGTGAACGAC CTCTCTCTTC CACTGGGCCT TCCCAGCATC TCCAGGCAGC TGGCTCTGGT
ATTCAGAATC AGAACGGACA TCCCACCCTG CCTAGCAATT CAGTAACACA GGGGGCTGCT
CTCAATCACC TCTCCTCTCA CACTGCTACC TCAGGTGGAC AACAAGGCAT TACCTTAACC
AAAGAGAGCA AGCCTTCAGG AAACATATTG ACGGTGCCTG AAACAAGCAG GCACACTGGA
GAGACACCTA ACAGCACTGC CAGTGTCGAG GGACTTCCTA ATCATGTCCA TCAGATGACG
GCAGATGCTG TTTGCAGTCC TAGCCATGGA GATTCTAAGT CACCAGGTTT ACTAAGTTCA
GACAATCCTC AGCTCTCTGC CTTGTTGATG GGAAAAGCCA ATAACAATGT GGGTACTGGA
ACCTGTGACA AAGTCAATAA CATCCACCCA GCTGTTCATA CAAAGACTGA TAACTCTGTT
GCCTCTTCAC CATCTTCAGC CATTTCAACA GCAACACCTT CTCCAAAATC CACTGAGCAG
ACAACCACAA ACAGTGTTAC CAGCCTTAAC AGCCCTCACA GTGGGCTACA CACAATTAAT
GGAGAAGGGA TGGAAGAATC TCAGAGCCCC ATGAAAACAG ATCTGCTTCT GGTTAACCAC
AAACCTAGTC CACAGATCAT ACCATCAATG TCTGTGTCCA TATACCCCAG CTCAGCAGAA
GTTCTGAAGG CATGCAGGAA TCTAGGTAAA AATGGCTTAT CTAACAGTAG CATTTTGTTG
GATAAATGTC CACCTCCAAG ACCACCATCT TCACCATACC CTCCCTTGCC AAAGGACAAG
TTGAATCCAC CTACACCTAG TATTTACTTG GAAAATAAAC GTGATGCTTT CTTTCCTCCA
TTACATCAAT TTTGTACAAA TCCGAACAAC CCTGTTACAG TAATACGTGG CCTTGCTGGA
GCTCTTAAGT TAGACCTGGG ACTTTTCTCT ACTAAAACTT TGGTGGAAGC TAACAATGAA
CATATGGTAG AAGTGAGGAC ACAGTTGTTG CAGCCAGCAG ATGAAAACTG GGATCCCACT
GGAACAAAGA AAATCTGGCA TTGTGAAAGT AATAGATCTC ATACTACAAT TGCTAAATAT
GCACAGTACC AGGCCTCCTC ATTCCAGGAA TCATTGAGAG AAGAAAATGA AAAAAGAAGT
CATCATAAAG ACCACTCAGA TAGTGAATCT ACATCGTCAG ATAATTCTGG GAGGAGGAGG
AAAGGACCCT TTAAAACCAT AAAGTTTGGG ACCAATATTG ACCTATCTGA TGACAAAAAG
TGGAAGTTGC AGCTACATGA GCTGACTAAA CTTCCTGCTT TTGTGCGTGT CGTATCAGCA
GGAAATCTTC TAAGCCATGT TGGTCATACC ATATTGGGCA TGAACACAGT TCAACTATAC
ATGAAAGTTC CAGGGAGCAG AACACCAGGT CATCAGGAAA ATAACAACTT CTGTTCAGTT
AACATAAATA TTGGCCCAGG TGACTGTGAA TGGTTTGTTG TTCCTGAAGG TTACTGGGGT
GTTCTGAATG ACTTCTGTGA AAAAAATAAT TTGAATTTCC TAATGGGTTC TTGGTGGCCC
AATCTTGAAG ATCTTTATGA AGCAAATGTT CCAGTGTATA GGTTTATTCA GCGACCTGGA
GATTTGGTCT GGATAAATGC AGGCACTGTT CATTGGGTTC AGGCTATTGG CTGGTGCAAC
AACATTGCTT GGAATGTTGG TCCACTTACA GCCTGCCAGT ATAAATTGGC AGTGGAACGG
TACGAATGGA ACAAATTGCA AAGTGTGAAG TCAATAGTAC CCATGGTTCA TCTTTCCTGG
AATATGGCAC GAAATATCAA GGTCTCAGAT CCAAAGCTTT TTGAAATGAT TAAGATATTT
TCATTGTCTC CGAATTTTAG AGCTGAAAAG TGCCTTAGAG ATCATCTAGT TCAACCTCTC
CGTTCAAATG GAGAACCTGA GCCACTAAGA TTCACAGGAG AGTATTGTCT TCTAAGAACT
CTGAAGCAAT GTCAGACATT GAGGGAAGCT CTCATTGCTG CAGGAAAAGA GATTATATGG
CATGGGCGGA CAAAAGAAGA ACCAGCTCAT TACTGTAGCA TTTGTGAAGT GGAGGTTTTT
GATCTGCTTT TTGTCACTAA TGAGAGTAAT TCACGAAAGA CCTACATAGT ACATTGCCAA
GATTGTGCAC GAAAAACAAG CGGAAACTTG GAAAACTTTG TGGTGCTAGA ACAGTACAAA
ATGGAGGACC TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCGCC
TCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61258
Clone ID Related Accession (Same CDS sequence) XM_011543967
Accession Version XM_011543967.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4263bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X11
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGAATA CTTCTGACAA TTGGAGTGGT GGACATGCTG TGTCACATCC TCCAGTACAG
CAACAAGCTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGCATTTGGA ACAGCTCCGC
GCAAATAGAA ATAATTTAAA TCCAGCACAG AAACTGATGC TGGAACAGCT GGAAAGTCAG
TTTGTCTTAA TGCAACAACA CCAAATGAGA CCAACAGGAG TTGCACAGGT ACGATCTACT
GGAATTCCTA ATGGGCCAAC AGCTGACTCA TCACTGCCTA CAAACTCAGT CTCTGGCCAG
CAGCCACAGC TTGCTCTGAC CAGAGTGCCT AGCGTCTCTC AGCCTGGAGT CCGTCCTGCC
TGCCCTGGGC AGCCTTTGGC CAATGGACCC TTTTCTGCAG GCCATGTTCC CTGTAGCACA
TCAAGAACGC TGGGAAGTAC AGACACTATT TTGATAGGCA ATAATCATAT AACAGGAAGT
GGAAGTAATG GAAACGTGCC TTACCTGCAG CGAAACGCAC TCACTCTACC TCATAACCGC
ACAAACCTGA CCAGCAGCGC AGAGGAGCCG TGGAAAAACC AACTATCTAA CTCCACTCAG
GGGCTTCACA AAGGTCAGAG TTCACATTCG GCAGGTCCTA ATGGTGAACG ACCTCTCTCT
TCCACTGGGC CTTCCCAGCA TCTCCAGGCA GCTGGCTCTG GTATTCAGAA TCAGAACGGA
CATCCCACCC TGCCTAGCAA TTCAGTAACA CAGGGGGCTG CTCTCAATCA CCTCTCCTCT
CACACTGCTA CCTCAGGTGG ACAACAAGGC ATTACCTTAA CCAAAGAGAG CAAGCCTTCA
GGAAACATAT TGACGGTGCC TGAAACAAGC AGGCACACTG GAGAGACACC TAACAGCACT
GCCAGTGTCG AGGGACTTCC TAATCATGTC CATCAGATGA CGGCAGATGC TGTTTGCAGT
CCTAGCCATG GAGATTCTAA GTCACCAGGT TTACTAAGTT CAGACAATCC TCAGCTCTCT
GCCTTGTTGA TGGGAAAAGC CAATAACAAT GTGGGTACTG GAACCTGTGA CAAAGTCAAT
AACATCCACC CAGCTGTTCA TACAAAGACT GATAACTCTG TTGCCTCTTC ACCATCTTCA
GCCATTTCAA CAGCAACACC TTCTCCAAAA TCCACTGAGC AGACAACCAC AAACAGTGTT
ACCAGCCTTA ACAGCCCTCA CAGTGGGCTA CACACAATTA ATGGAGAAGG GATGGAAGAA
TCTCAGAGCC CCATGAAAAC AGATCTGCTT CTGGTTAACC ACAAACCTAG TCCACAGATC
ATACCATCAA TGTCTGTGTC CATATACCCC AGCTCAGCAG AAGTTCTGAA GGCATGCAGG
AATCTAGGTA AAAATGGCTT ATCTAACAGT AGCATTTTGT TGGATAAATG TCCACCTCCA
AGACCACCAT CTTCACCATA CCCTCCCTTG CCAAAGGACA AGTTGAATCC ACCTACACCT
AGTATTTACT TGGAAAATAA ACGTGATGCT TTCTTTCCTC CATTACATCA ATTTTGTACA
AATCCGAACA ACCCTGTTAC AGTAATACGT GGCCTTGCTG GAGCTCTTAA GTTAGACCTG
GGACTTTTCT CTACTAAAAC TTTGGTGGAA GCTAACAATG AACATATGGT AGAAGTGAGG
ACACAGTTGT TGCAGCCAGC AGATGAAAAC TGGGATCCCA CTGGAACAAA GAAAATCTGG
CATTGTGAAA GTAATAGATC TCATACTACA ATTGCTAAAT ATGCACAGTA CCAGGCCTCC
TCATTCCAGG AATCATTGAG AGAAGAAAAT GAAAAAAGAA GTCATCATAA AGACCACTCA
GATAGTGAAT CTACATCGTC AGATAATTCT GGGAGGAGGA GGAAAGGACC CTTTAAAACC
ATAAAGTTTG GGACCAATAT TGACCTATCT GATGACAAAA AGTGGAAGTT GCAGCTACAT
GAGCTGACTA AACTTCCTGC TTTTGTGCGT GTCGTATCAG CAGGAAATCT TCTAAGCCAT
GTTGGTCATA CCATATTGGG CATGAACACA GTTCAACTAT ACATGAAAGT TCCAGGGAGC
AGAACACCAG GTCATCAGGA AAATAACAAC TTCTGTTCAG TTAACATAAA TATTGGCCCA
GGTGACTGTG AATGGTTTGT TGTTCCTGAA GGTTACTGGG GTGTTCTGAA TGACTTCTGT
GAAAAAAATA ATTTGAATTT CCTAATGGGT TCTTGGTGGC CCAATCTTGA AGATCTTTAT
GAAGCAAATG TTCCAGTGTA TAGGTTTATT CAGCGACCTG GAGATTTGGT CTGGATAAAT
GCAGGCACTG TTCATTGGGT TCAGGCTATT GGCTGGTGCA ACAACATTGC TTGGAATGTT
GGTCCACTTA CAGCCTGCCA GTATAAATTG GCAGTGGAAC GGTACGAATG GAACAAATTG
CAAAGTGTGA AGTCAATAGT ACCCATGGTT CATCTTTCCT GGAATATGGC ACGAAATATC
AAGGTCTCAG ATCCAAAGCT TTTTGAAATG ATTAAGTATT GTCTTCTAAG AACTCTGAAG
CAATGTCAGA CATTGAGGGA AGCTCTCATT GCTGCAGGAA AAGAGATTAT ATGGCATGGG
CGGACAAAAG AAGAACCAGC TCATTACTGT AGCATTTGTG AAGTGGAGGT TTTTGATCTG
CTTTTTGTCA CTAATGAGAG TAATTCACGA AAGACCTACA TAGTACATTG CCAAGATTGT
GCACGAAAAA CAAGCGGAAA CTTGGAAAAC TTTGTGGTGC TAGAACAGTA CAAAATGGAG
GACCTGATGC AAGTCTATGA CCAATTTACA TTAGCTCCTC CATTACCATC CGCCTCATCT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542269.1
CDS378..4640
Misc Feature(1)654..740(+)
Misc Feature(2)657..971(+)
Misc Feature(3)810..1631(+)
Misc Feature(4)819..911(+)
Misc Feature(5)822..923(+)
Misc Feature(6)924..1016(+)
Misc Feature(7)1047..1133(+)
Misc Feature(8)1050..1322(+)
Misc Feature(9)1146..1271(+)
Misc Feature(10)1248..1343(+)
Misc Feature(11)1284..1370(+)
Misc Feature(12)1287..1526(+)
Misc Feature(13)1287..1370(+)
Misc Feature(14)1383..1475(+)
Misc Feature(15)1386..1487(+)
Misc Feature(16)1488..1568(+)
Misc Feature(17)3729..3923(+)
Misc Feature(18)3831..4157(+)
Translation

Target ORF information:

RefSeq Version XM_011543967
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543967

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGAATA CTTCTGACAA TTGGAGTGGT GGACATGCTG TGTCACATCC TCCAGTACAG
CAACAAGCTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGCATTTGGA ACAGCTCCGC
GCAAATAGAA ATAATTTAAA TCCAGCACAG AAACTGATGC TGGAACAGCT GGAAAGTCAG
TTTGTCTTAA TGCAACAACA CCAAATGAGA CCAACAGGAG TTGCACAGGT ACGATCTACT
GGAATTCCTA ATGGGCCAAC AGCTGACTCA TCACTGCCTA CAAACTCAGT CTCTGGCCAG
CAGCCACAGC TTGCTCTGAC CAGAGTGCCT AGCGTCTCTC AGCCTGGAGT CCGTCCTGCC
TGCCCTGGGC AGCCTTTGGC CAATGGACCC TTTTCTGCAG GCCATGTTCC CTGTAGCACA
TCAAGAACGC TGGGAAGTAC AGACACTATT TTGATAGGCA ATAATCATAT AACAGGAAGT
GGAAGTAATG GAAACGTGCC TTACCTGCAG CGAAACGCAC TCACTCTACC TCATAACCGC
ACAAACCTGA CCAGCAGCGC AGAGGAGCCG TGGAAAAACC AACTATCTAA CTCCACTCAG
GGGCTTCACA AAGGTCAGAG TTCACATTCG GCAGGTCCTA ATGGTGAACG ACCTCTCTCT
TCCACTGGGC CTTCCCAGCA TCTCCAGGCA GCTGGCTCTG GTATTCAGAA TCAGAACGGA
CATCCCACCC TGCCTAGCAA TTCAGTAACA CAGGGGGCTG CTCTCAATCA CCTCTCCTCT
CACACTGCTA CCTCAGGTGG ACAACAAGGC ATTACCTTAA CCAAAGAGAG CAAGCCTTCA
GGAAACATAT TGACGGTGCC TGAAACAAGC AGGCACACTG GAGAGACACC TAACAGCACT
GCCAGTGTCG AGGGACTTCC TAATCATGTC CATCAGATGA CGGCAGATGC TGTTTGCAGT
CCTAGCCATG GAGATTCTAA GTCACCAGGT TTACTAAGTT CAGACAATCC TCAGCTCTCT
GCCTTGTTGA TGGGAAAAGC CAATAACAAT GTGGGTACTG GAACCTGTGA CAAAGTCAAT
AACATCCACC CAGCTGTTCA TACAAAGACT GATAACTCTG TTGCCTCTTC ACCATCTTCA
GCCATTTCAA CAGCAACACC TTCTCCAAAA TCCACTGAGC AGACAACCAC AAACAGTGTT
ACCAGCCTTA ACAGCCCTCA CAGTGGGCTA CACACAATTA ATGGAGAAGG GATGGAAGAA
TCTCAGAGCC CCATGAAAAC AGATCTGCTT CTGGTTAACC ACAAACCTAG TCCACAGATC
ATACCATCAA TGTCTGTGTC CATATACCCC AGCTCAGCAG AAGTTCTGAA GGCATGCAGG
AATCTAGGTA AAAATGGCTT ATCTAACAGT AGCATTTTGT TGGATAAATG TCCACCTCCA
AGACCACCAT CTTCACCATA CCCTCCCTTG CCAAAGGACA AGTTGAATCC ACCTACACCT
AGTATTTACT TGGAAAATAA ACGTGATGCT TTCTTTCCTC CATTACATCA ATTTTGTACA
AATCCGAACA ACCCTGTTAC AGTAATACGT GGCCTTGCTG GAGCTCTTAA GTTAGACCTG
GGACTTTTCT CTACTAAAAC TTTGGTGGAA GCTAACAATG AACATATGGT AGAAGTGAGG
ACACAGTTGT TGCAGCCAGC AGATGAAAAC TGGGATCCCA CTGGAACAAA GAAAATCTGG
CATTGTGAAA GTAATAGATC TCATACTACA ATTGCTAAAT ATGCACAGTA CCAGGCCTCC
TCATTCCAGG AATCATTGAG AGAAGAAAAT GAAAAAAGAA GTCATCATAA AGACCACTCA
GATAGTGAAT CTACATCGTC AGATAATTCT GGGAGGAGGA GGAAAGGACC CTTTAAAACC
ATAAAGTTTG GGACCAATAT TGACCTATCT GATGACAAAA AGTGGAAGTT GCAGCTACAT
GAGCTGACTA AACTTCCTGC TTTTGTGCGT GTCGTATCAG CAGGAAATCT TCTAAGCCAT
GTTGGTCATA CCATATTGGG CATGAACACA GTTCAACTAT ACATGAAAGT TCCAGGGAGC
AGAACACCAG GTCATCAGGA AAATAACAAC TTCTGTTCAG TTAACATAAA TATTGGCCCA
GGTGACTGTG AATGGTTTGT TGTTCCTGAA GGTTACTGGG GTGTTCTGAA TGACTTCTGT
GAAAAAAATA ATTTGAATTT CCTAATGGGT TCTTGGTGGC CCAATCTTGA AGATCTTTAT
GAAGCAAATG TTCCAGTGTA TAGGTTTATT CAGCGACCTG GAGATTTGGT CTGGATAAAT
GCAGGCACTG TTCATTGGGT TCAGGCTATT GGCTGGTGCA ACAACATTGC TTGGAATGTT
GGTCCACTTA CAGCCTGCCA GTATAAATTG GCAGTGGAAC GGTACGAATG GAACAAATTG
CAAAGTGTGA AGTCAATAGT ACCCATGGTT CATCTTTCCT GGAATATGGC ACGAAATATC
AAGGTCTCAG ATCCAAAGCT TTTTGAAATG ATTAAGTATT GTCTTCTAAG AACTCTGAAG
CAATGTCAGA CATTGAGGGA AGCTCTCATT GCTGCAGGAA AAGAGATTAT ATGGCATGGG
CGGACAAAAG AAGAACCAGC TCATTACTGT AGCATTTGTG AAGTGGAGGT TTTTGATCTG
CTTTTTGTCA CTAATGAGAG TAATTCACGA AAGACCTACA TAGTACATTG CCAAGATTGT
GCACGAAAAA CAAGCGGAAA CTTGGAAAAC TTTGTGGTGC TAGAACAGTA CAAAATGGAG
GACCTGATGC AAGTCTATGA CCAATTTACA TTAGCTCCTC CATTACCATC CGCCTCATCT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54022
Clone ID Related Accession (Same CDS sequence) XM_005272656
Accession Version XM_005272656.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838075. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT
TTGACACCAC AGAAATTACA GATGAGACCA ACAGGAGTTG CACAGGTACG ATCTACTGGA
ATTCCTAATG GGCCAACAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG
CCACAGCTTG CTCTGACCAG AGTGCCTAGC GTCTCTCAGC CTGGAGTCCG TCCTGCCTGC
CCTGGGCAGC CTTTGGCCAA TGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA
AGAACGCTGG GAAGTACAGA CACTATTTTG ATAGGCAATA ATCATATAAC AGGAAGTGGA
AGTAATGGAA ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA
AACCTGACCA GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG
CTTCACAAAG GTCAGAGTTC ACATTCGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC
ACTGGGCCTT CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAACGGACAT
CCCACCCTGC CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC
ACTGCTACCT CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA
AACATATTGA CGGTGCCTGA AACAAGCAGG CACACTGGAG AGACACCTAA CAGCACTGCC
AGTGTCGAGG GACTTCCTAA TCATGTCCAT CAGATGACGG CAGATGCTGT TTGCAGTCCT
AGCCATGGAG ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC
TTGTTGATGG GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC
ATCCACCCAG CTGTTCATAC AAAGACTGAT AACTCTGTTG CCTCTTCACC ATCTTCAGCC
ATTTCAACAG CAACACCTTC TCCAAAATCC ACTGAGCAGA CAACCACAAA CAGTGTTACC
AGCCTTAACA GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGGAT GGAAGAATCT
CAGAGCCCCA TGAAAACAGA TCTGCTTCTG GTTAACCACA AACCTAGTCC ACAGATCATA
CCATCAATGT CTGTGTCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAT
CTAGGTAAAA ATGGCTTATC TAACAGTAGC ATTTTGTTGG ATAAATGTCC ACCTCCAAGA
CCACCATCTT CACCATACCC TCCCTTGCCA AAGGACAAGT TGAATCCACC TACACCTAGT
ATTTACTTGG AAAATAAACG TGATGCTTTC TTTCCTCCAT TACATCAATT TTGTACAAAT
CCGAACAACC CTGTTACAGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT AGACCTGGGA
CTTTTCTCTA CTAAAACTTT GGTGGAAGCT AACAATGAAC ATATGGTAGA AGTGAGGACA
CAGTTGTTGC AGCCAGCAGA TGAAAACTGG GATCCCACTG GAACAAAGAA AATCTGGCAT
TGTGAAAGTA ATAGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCA
TTCCAGGAAT CATTGAGAGA AGAAAATGAA AAAAGAAGTC ATCATAAAGA CCACTCAGAT
AGTGAATCTA CATCGTCAGA TAATTCTGGG AGGAGGAGGA AAGGACCCTT TAAAACCATA
AAGTTTGGGA CCAATATTGA CCTATCTGAT GACAAAAAGT GGAAGTTGCA GCTACATGAG
CTGACTAAAC TTCCTGCTTT TGTGCGTGTC GTATCAGCAG GAAATCTTCT AAGCCATGTT
GGTCATACCA TATTGGGCAT GAACACAGTT CAACTATACA TGAAAGTTCC AGGGAGCAGA
ACACCAGGTC ATCAGGAAAA TAACAACTTC TGTTCAGTTA ACATAAATAT TGGCCCAGGT
GACTGTGAAT GGTTTGTTGT TCCTGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA
AAAAATAATT TGAATTTCCT AATGGGTTCT TGGTGGCCCA ATCTTGAAGA TCTTTATGAA
GCAAATGTTC CAGTGTATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA
GGCACTGTTC ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT
CCACTTACAG CCTGCCAGTA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA
AGTGTGAAGT CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG
GTCTCAGATC CAAAGCTTTT TGAAATGATT AAGTATTGTC TTCTAAGAAC TCTGAAGCAA
TGTCAGACAT TGAGGGAAGC TCTCATTGCT GCAGGAAAAG AGATTATATG GCATGGGCGG
ACAAAAGAAG AACCAGCTCA TTACTGTAGC ATTTGTGAAG TGGAGGTTTT TGATCTGCTT
TTTGTCACTA ATGAGAGTAA TTCACGAAAG ACCTACATAG TACATTGCCA AGATTGTGCA
CGAAAAACAA GCGGAAACTT GGAAAACTTT GTGGTGCTAG AACAGTACAA AATGGAGGAC
CTGATGCAAG TCTATGACCA ATTTACATTA GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005272713.1
CDS378..4637
Misc Feature(1)654..740(+)
Misc Feature(2)657..914(+)
Misc Feature(3)693..1574(+)
Misc Feature(4)762..854(+)
Misc Feature(5)765..866(+)
Misc Feature(6)867..959(+)
Misc Feature(7)990..1076(+)
Misc Feature(8)993..1265(+)
Misc Feature(9)1089..1214(+)
Misc Feature(10)1191..1286(+)
Misc Feature(11)1227..1313(+)
Misc Feature(12)1230..1469(+)
Misc Feature(13)1230..1313(+)
Misc Feature(14)1326..1418(+)
Misc Feature(15)1329..1430(+)
Misc Feature(16)1431..1511(+)
Misc Feature(17)3726..3920(+)
Misc Feature(18)3828..4154(+)
Translation

Target ORF information:

RefSeq Version XM_005272656
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005272656

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT
TTGACACCAC AGAAATTACA GATGAGACCA ACAGGAGTTG CACAGGTACG ATCTACTGGA
ATTCCTAATG GGCCAACAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG
CCACAGCTTG CTCTGACCAG AGTGCCTAGC GTCTCTCAGC CTGGAGTCCG TCCTGCCTGC
CCTGGGCAGC CTTTGGCCAA TGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA
AGAACGCTGG GAAGTACAGA CACTATTTTG ATAGGCAATA ATCATATAAC AGGAAGTGGA
AGTAATGGAA ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA
AACCTGACCA GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG
CTTCACAAAG GTCAGAGTTC ACATTCGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC
ACTGGGCCTT CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAACGGACAT
CCCACCCTGC CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC
ACTGCTACCT CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA
AACATATTGA CGGTGCCTGA AACAAGCAGG CACACTGGAG AGACACCTAA CAGCACTGCC
AGTGTCGAGG GACTTCCTAA TCATGTCCAT CAGATGACGG CAGATGCTGT TTGCAGTCCT
AGCCATGGAG ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC
TTGTTGATGG GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC
ATCCACCCAG CTGTTCATAC AAAGACTGAT AACTCTGTTG CCTCTTCACC ATCTTCAGCC
ATTTCAACAG CAACACCTTC TCCAAAATCC ACTGAGCAGA CAACCACAAA CAGTGTTACC
AGCCTTAACA GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGGAT GGAAGAATCT
CAGAGCCCCA TGAAAACAGA TCTGCTTCTG GTTAACCACA AACCTAGTCC ACAGATCATA
CCATCAATGT CTGTGTCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAT
CTAGGTAAAA ATGGCTTATC TAACAGTAGC ATTTTGTTGG ATAAATGTCC ACCTCCAAGA
CCACCATCTT CACCATACCC TCCCTTGCCA AAGGACAAGT TGAATCCACC TACACCTAGT
ATTTACTTGG AAAATAAACG TGATGCTTTC TTTCCTCCAT TACATCAATT TTGTACAAAT
CCGAACAACC CTGTTACAGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT AGACCTGGGA
CTTTTCTCTA CTAAAACTTT GGTGGAAGCT AACAATGAAC ATATGGTAGA AGTGAGGACA
CAGTTGTTGC AGCCAGCAGA TGAAAACTGG GATCCCACTG GAACAAAGAA AATCTGGCAT
TGTGAAAGTA ATAGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCA
TTCCAGGAAT CATTGAGAGA AGAAAATGAA AAAAGAAGTC ATCATAAAGA CCACTCAGAT
AGTGAATCTA CATCGTCAGA TAATTCTGGG AGGAGGAGGA AAGGACCCTT TAAAACCATA
AAGTTTGGGA CCAATATTGA CCTATCTGAT GACAAAAAGT GGAAGTTGCA GCTACATGAG
CTGACTAAAC TTCCTGCTTT TGTGCGTGTC GTATCAGCAG GAAATCTTCT AAGCCATGTT
GGTCATACCA TATTGGGCAT GAACACAGTT CAACTATACA TGAAAGTTCC AGGGAGCAGA
ACACCAGGTC ATCAGGAAAA TAACAACTTC TGTTCAGTTA ACATAAATAT TGGCCCAGGT
GACTGTGAAT GGTTTGTTGT TCCTGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA
AAAAATAATT TGAATTTCCT AATGGGTTCT TGGTGGCCCA ATCTTGAAGA TCTTTATGAA
GCAAATGTTC CAGTGTATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA
GGCACTGTTC ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT
CCACTTACAG CCTGCCAGTA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA
AGTGTGAAGT CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG
GTCTCAGATC CAAAGCTTTT TGAAATGATT AAGTATTGTC TTCTAAGAAC TCTGAAGCAA
TGTCAGACAT TGAGGGAAGC TCTCATTGCT GCAGGAAAAG AGATTATATG GCATGGGCGG
ACAAAAGAAG AACCAGCTCA TTACTGTAGC ATTTGTGAAG TGGAGGTTTT TGATCTGCTT
TTTGTCACTA ATGAGAGTAA TTCACGAAAG ACCTACATAG TACATTGCCA AGATTGTGCA
CGAAAAACAA GCGGAAACTT GGAAAACTTT GTGGTGCTAG AACAGTACAA AATGGAGGAC
CTGATGCAAG TCTATGACCA ATTTACATTA GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61259
Clone ID Related Accession (Same CDS sequence) XM_011543968
Accession Version XM_011543968.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X13
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GAATACTTCT
GACAATTGGA GTGGTGGACA TGCTGTGTCA CATCCTCCAG TACAGCAACA AGCTCATTCA
TGGTGTTTGA CACCACAGAA ATTACAGCAT TTGGAACAGC TCCGCGCAAA TAGAAATAAT
TTAAATCCAG CACAGAAACT GATGCTGGAA CAGCTGGAAA GTCAGTTTGT CTTAATGCAA
CAACACCAAA TGAGACCAAC AGGAGTTGCA CAGGTACGAT CTACTGGAAT TCCTAATGGG
CCAACAGCTG ACTCATCACT GCCTACAAAC TCAGTCTCTG GCCAGCAGCC ACAGCTTGCT
CTGACCAGAG TGCCTAGCGT CTCTCAGCCT GGAGTCCGTC CTGCCTGCCC TGGGCAGCCT
TTGGCCAATG GACCCTTTTC TGCAGGCCAT GTTCCCTGTA GCACATCAAG AACGCTGGGA
AGTACAGACA CTATTTTGAT AGGCAATAAT CATATAACAG GAAGTGGAAG TAATGGAAAC
GTGCCTTACC TGCAGCGAAA CGCACTCACT CTACCTCATA ACCGCACAAA CCTGACCAGC
AGCGCAGAGG AGCCGTGGAA AAACCAACTA TCTAACTCCA CTCAGGGGCT TCACAAAGGT
CAGAGTTCAC ATTCGGCAGG TCCTAATGGT GAACGACCTC TCTCTTCCAC TGGGCCTTCC
CAGCATCTCC AGGCAGCTGG CTCTGGTATT CAGAATCAGA ACGGACATCC CACCCTGCCT
AGCAATTCAG TAACACAGGG GGCTGCTCTC AATCACCTCT CCTCTCACAC TGCTACCTCA
GGTGGACAAC AAGGCATTAC CTTAACCAAA GAGAGCAAGC CTTCAGGAAA CATATTGACG
GTGCCTGAAA CAAGCAGGCA CACTGGAGAG ACACCTAACA GCACTGCCAG TGTCGAGGGA
CTTCCTAATC ATGTCCATCA GATGACGGCA GATGCTGTTT GCAGTCCTAG CCATGGAGAT
TCTAAGTCAC CAGGTTTACT AAGTTCAGAC AATCCTCAGC TCTCTGCCTT GTTGATGGGA
AAAGCCAATA ACAATGTGGG TACTGGAACC TGTGACAAAG TCAATAACAT CCACCCAGCT
GTTCATACAA AGACTGATAA CTCTGTTGCC TCTTCACCAT CTTCAGCCAT TTCAACAGCA
ACACCTTCTC CAAAATCCAC TGAGCAGACA ACCACAAACA GTGTTACCAG CCTTAACAGC
CCTCACAGTG GGCTACACAC AATTAATGGA GAAGGGATGG AAGAATCTCA GAGCCCCATG
AAAACAGATC TGCTTCTGGT TAACCACAAA CCTAGTCCAC AGATCATACC ATCAATGTCT
GTGTCCATAT ACCCCAGCTC AGCAGAAGTT CTGAAGGCAT GCAGGAATCT AGGTAAAAAT
GGCTTATCTA ACAGTAGCAT TTTGTTGGAT AAATGTCCAC CTCCAAGACC ACCATCTTCA
CCATACCCTC CCTTGCCAAA GGACAAGTTG AATCCACCTA CACCTAGTAT TTACTTGGAA
AATAAACGTG ATGCTTTCTT TCCTCCATTA CATCAATTTT GTACAAATCC GAACAACCCT
GTTACAGTAA TACGTGGCCT TGCTGGAGCT CTTAAGTTAG ACCTGGGACT TTTCTCTACT
AAAACTTTGG TGGAAGCTAA CAATGAACAT ATGGTAGAAG TGAGGACACA GTTGTTGCAG
CCAGCAGATG AAAACTGGGA TCCCACTGGA ACAAAGAAAA TCTGGCATTG TGAAAGTAAT
AGATCTCATA CTACAATTGC TAAATATGCA CAGTACCAGG CCTCCTCATT CCAGGAATCA
TTGAGAGAAG AAAATGAAAA AAGAAGTCAT CATAAAGACC ACTCAGATAG TGAATCTACA
TCGTCAGATA ATTCTGGGAG GAGGAGGAAA GGACCCTTTA AAACCATAAA GTTTGGGACC
AATATTGACC TATCTGATGA CAAAAAGTGG AAGTTGCAGC TACATGAGCT GACTAAACTT
CCTGCTTTTG TGCGTGTCGT ATCAGCAGGA AATCTTCTAA GCCATGTTGG TCATACCATA
TTGGGCATGA ACACAGTTCA ACTATACATG AAAGTTCCAG GGAGCAGAAC ACCAGGTCAT
CAGGAAAATA ACAACTTCTG TTCAGTTAAC ATAAATATTG GCCCAGGTGA CTGTGAATGG
TTTGTTGTTC CTGAAGGTTA CTGGGGTGTT CTGAATGACT TCTGTGAAAA AAATAATTTG
AATTTCCTAA TGGGTTCTTG GTGGCCCAAT CTTGAAGATC TTTATGAAGC AAATGTTCCA
GTGTATAGGT TTATTCAGCG ACCTGGAGAT TTGGTCTGGA TAAATGCAGG CACTGTTCAT
TGGGTTCAGG CTATTGGCTG GTGCAACAAC ATTGCTTGGA ATGTTGGTCC ACTTACAGCC
TGCCAGTATA AATTGGCAGT GGAACGGTAC GAATGGAACA AATTGCAAAG TGTGAAGTCA
ATAGTACCCA TGGTTCATCT TTCCTGGAAT ATGGCACGAA ATATCAAGGT CTCAGATCCA
AAGCTTTTTG AAATGATTAA GATATTTTCA TTGTCTCCGA ATTTTAGAGC TGAAAAGTGC
CTTAGAGATC ATCTAGTTCA ACCTCTCCGT TCAAATGGAG AACCTGAGCC ACTAAGATTC
ACAGGAGAGT ATTGTCTTCT AAGAACTCTG AAGCAATGTC AGACATTGAG GGAAGCTCTC
ATTGCTGCAG GAAAAGAGAT TATATGGCAT GGGCGGACAA AAGAAGAACC AGCTCATTAC
TGTAGCATTT GTGAAGTGGA GGTTTTTGAT CTGCTTTTTG TCACTAATGA GAGTAATTCA
CGAAAGACCT ACATAGTACA TTGCCAAGAT TGTGCACGAA AAACAAGCGG AAACTTGGAA
AACTTTGTGG TGCTAGAACA GTACAAAATG GAGGACCTGA TGCAAGTCTA TGACCAATTT
ACATTAGCTC CTCCATTACC ATCCGCCTCA TCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542270.1
CDS378..4613
Misc Feature(1)654..740(+)
Misc Feature(2)657..971(+)
Misc Feature(3)819..1016(+)
Misc Feature(4)819..911(+)
Misc Feature(5)822..923(+)
Misc Feature(6)924..1016(+)
Misc Feature(7)1047..1280(+)
Misc Feature(8)1047..1133(+)
Misc Feature(9)1050..1322(+)
Misc Feature(10)1146..1271(+)
Misc Feature(11)1248..1343(+)
Misc Feature(12)1278..1478(+)
Misc Feature(13)1284..1370(+)
Misc Feature(14)1287..1526(+)
Misc Feature(15)1287..1370(+)
Misc Feature(16)1383..1475(+)
Misc Feature(17)1386..1487(+)
Misc Feature(18)1488..1568(+)
Misc Feature(19)3594..3788(+)
Misc Feature(20)3696..4022(+)
Translation

Target ORF information:

RefSeq Version XM_011543968
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543968

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GAATACTTCT
GACAATTGGA GTGGTGGACA TGCTGTGTCA CATCCTCCAG TACAGCAACA AGCTCATTCA
TGGTGTTTGA CACCACAGAA ATTACAGCAT TTGGAACAGC TCCGCGCAAA TAGAAATAAT
TTAAATCCAG CACAGAAACT GATGCTGGAA CAGCTGGAAA GTCAGTTTGT CTTAATGCAA
CAACACCAAA TGAGACCAAC AGGAGTTGCA CAGGTACGAT CTACTGGAAT TCCTAATGGG
CCAACAGCTG ACTCATCACT GCCTACAAAC TCAGTCTCTG GCCAGCAGCC ACAGCTTGCT
CTGACCAGAG TGCCTAGCGT CTCTCAGCCT GGAGTCCGTC CTGCCTGCCC TGGGCAGCCT
TTGGCCAATG GACCCTTTTC TGCAGGCCAT GTTCCCTGTA GCACATCAAG AACGCTGGGA
AGTACAGACA CTATTTTGAT AGGCAATAAT CATATAACAG GAAGTGGAAG TAATGGAAAC
GTGCCTTACC TGCAGCGAAA CGCACTCACT CTACCTCATA ACCGCACAAA CCTGACCAGC
AGCGCAGAGG AGCCGTGGAA AAACCAACTA TCTAACTCCA CTCAGGGGCT TCACAAAGGT
CAGAGTTCAC ATTCGGCAGG TCCTAATGGT GAACGACCTC TCTCTTCCAC TGGGCCTTCC
CAGCATCTCC AGGCAGCTGG CTCTGGTATT CAGAATCAGA ACGGACATCC CACCCTGCCT
AGCAATTCAG TAACACAGGG GGCTGCTCTC AATCACCTCT CCTCTCACAC TGCTACCTCA
GGTGGACAAC AAGGCATTAC CTTAACCAAA GAGAGCAAGC CTTCAGGAAA CATATTGACG
GTGCCTGAAA CAAGCAGGCA CACTGGAGAG ACACCTAACA GCACTGCCAG TGTCGAGGGA
CTTCCTAATC ATGTCCATCA GATGACGGCA GATGCTGTTT GCAGTCCTAG CCATGGAGAT
TCTAAGTCAC CAGGTTTACT AAGTTCAGAC AATCCTCAGC TCTCTGCCTT GTTGATGGGA
AAAGCCAATA ACAATGTGGG TACTGGAACC TGTGACAAAG TCAATAACAT CCACCCAGCT
GTTCATACAA AGACTGATAA CTCTGTTGCC TCTTCACCAT CTTCAGCCAT TTCAACAGCA
ACACCTTCTC CAAAATCCAC TGAGCAGACA ACCACAAACA GTGTTACCAG CCTTAACAGC
CCTCACAGTG GGCTACACAC AATTAATGGA GAAGGGATGG AAGAATCTCA GAGCCCCATG
AAAACAGATC TGCTTCTGGT TAACCACAAA CCTAGTCCAC AGATCATACC ATCAATGTCT
GTGTCCATAT ACCCCAGCTC AGCAGAAGTT CTGAAGGCAT GCAGGAATCT AGGTAAAAAT
GGCTTATCTA ACAGTAGCAT TTTGTTGGAT AAATGTCCAC CTCCAAGACC ACCATCTTCA
CCATACCCTC CCTTGCCAAA GGACAAGTTG AATCCACCTA CACCTAGTAT TTACTTGGAA
AATAAACGTG ATGCTTTCTT TCCTCCATTA CATCAATTTT GTACAAATCC GAACAACCCT
GTTACAGTAA TACGTGGCCT TGCTGGAGCT CTTAAGTTAG ACCTGGGACT TTTCTCTACT
AAAACTTTGG TGGAAGCTAA CAATGAACAT ATGGTAGAAG TGAGGACACA GTTGTTGCAG
CCAGCAGATG AAAACTGGGA TCCCACTGGA ACAAAGAAAA TCTGGCATTG TGAAAGTAAT
AGATCTCATA CTACAATTGC TAAATATGCA CAGTACCAGG CCTCCTCATT CCAGGAATCA
TTGAGAGAAG AAAATGAAAA AAGAAGTCAT CATAAAGACC ACTCAGATAG TGAATCTACA
TCGTCAGATA ATTCTGGGAG GAGGAGGAAA GGACCCTTTA AAACCATAAA GTTTGGGACC
AATATTGACC TATCTGATGA CAAAAAGTGG AAGTTGCAGC TACATGAGCT GACTAAACTT
CCTGCTTTTG TGCGTGTCGT ATCAGCAGGA AATCTTCTAA GCCATGTTGG TCATACCATA
TTGGGCATGA ACACAGTTCA ACTATACATG AAAGTTCCAG GGAGCAGAAC ACCAGGTCAT
CAGGAAAATA ACAACTTCTG TTCAGTTAAC ATAAATATTG GCCCAGGTGA CTGTGAATGG
TTTGTTGTTC CTGAAGGTTA CTGGGGTGTT CTGAATGACT TCTGTGAAAA AAATAATTTG
AATTTCCTAA TGGGTTCTTG GTGGCCCAAT CTTGAAGATC TTTATGAAGC AAATGTTCCA
GTGTATAGGT TTATTCAGCG ACCTGGAGAT TTGGTCTGGA TAAATGCAGG CACTGTTCAT
TGGGTTCAGG CTATTGGCTG GTGCAACAAC ATTGCTTGGA ATGTTGGTCC ACTTACAGCC
TGCCAGTATA AATTGGCAGT GGAACGGTAC GAATGGAACA AATTGCAAAG TGTGAAGTCA
ATAGTACCCA TGGTTCATCT TTCCTGGAAT ATGGCACGAA ATATCAAGGT CTCAGATCCA
AAGCTTTTTG AAATGATTAA GATATTTTCA TTGTCTCCGA ATTTTAGAGC TGAAAAGTGC
CTTAGAGATC ATCTAGTTCA ACCTCTCCGT TCAAATGGAG AACCTGAGCC ACTAAGATTC
ACAGGAGAGT ATTGTCTTCT AAGAACTCTG AAGCAATGTC AGACATTGAG GGAAGCTCTC
ATTGCTGCAG GAAAAGAGAT TATATGGCAT GGGCGGACAA AAGAAGAACC AGCTCATTAC
TGTAGCATTT GTGAAGTGGA GGTTTTTGAT CTGCTTTTTG TCACTAATGA GAGTAATTCA
CGAAAGACCT ACATAGTACA TTGCCAAGAT TGTGCACGAA AAACAAGCGG AAACTTGGAA
AACTTTGTGG TGCTAGAACA GTACAAAATG GAGGACCTGA TGCAAGTCTA TGACCAATTT
ACATTAGCTC CTCCATTACC ATCCGCCTCA TCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61260
Clone ID Related Accession (Same CDS sequence) XM_011543969
Accession Version XM_011543969.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X14
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA
TTACAGATGA GACCAACAGG AGTTGCACAG GTACGATCTA CTGGAATTCC TAATGGGCCA
ACAGCTGACT CATCACTGCC TACAAACTCA GTCTCTGGCC AGCAGCCACA GCTTGCTCTG
ACCAGAGTGC CTAGCGTCTC TCAGCCTGGA GTCCGTCCTG CCTGCCCTGG GCAGCCTTTG
GCCAATGGAC CCTTTTCTGC AGGCCATGTT CCCTGTAGCA CATCAAGAAC GCTGGGAAGT
ACAGACACTA TTTTGATAGG CAATAATCAT ATAACAGGAA GTGGAAGTAA TGGAAACGTG
CCTTACCTGC AGCGAAACGC ACTCACTCTA CCTCATAACC GCACAAACCT GACCAGCAGC
GCAGAGGAGC CGTGGAAAAA CCAACTATCT AACTCCACTC AGGGGCTTCA CAAAGGTCAG
AGTTCACATT CGGCAGGTCC TAATGGTGAA CGACCTCTCT CTTCCACTGG GCCTTCCCAG
CATCTCCAGG CAGCTGGCTC TGGTATTCAG AATCAGAACG GACATCCCAC CCTGCCTAGC
AATTCAGTAA CACAGGGGGC TGCTCTCAAT CACCTCTCCT CTCACACTGC TACCTCAGGT
GGACAACAAG GCATTACCTT AACCAAAGAG AGCAAGCCTT CAGGAAACAT ATTGACGGTG
CCTGAAACAA GCAGGCACAC TGGAGAGACA CCTAACAGCA CTGCCAGTGT CGAGGGACTT
CCTAATCATG TCCATCAGAT GACGGCAGAT GCTGTTTGCA GTCCTAGCCA TGGAGATTCT
AAGTCACCAG GTTTACTAAG TTCAGACAAT CCTCAGCTCT CTGCCTTGTT GATGGGAAAA
GCCAATAACA ATGTGGGTAC TGGAACCTGT GACAAAGTCA ATAACATCCA CCCAGCTGTT
CATACAAAGA CTGATAACTC TGTTGCCTCT TCACCATCTT CAGCCATTTC AACAGCAACA
CCTTCTCCAA AATCCACTGA GCAGACAACC ACAAACAGTG TTACCAGCCT TAACAGCCCT
CACAGTGGGC TACACACAAT TAATGGAGAA GGGATGGAAG AATCTCAGAG CCCCATGAAA
ACAGATCTGC TTCTGGTTAA CCACAAACCT AGTCCACAGA TCATACCATC AATGTCTGTG
TCCATATACC CCAGCTCAGC AGAAGTTCTG AAGGCATGCA GGAATCTAGG TAAAAATGGC
TTATCTAACA GTAGCATTTT GTTGGATAAA TGTCCACCTC CAAGACCACC ATCTTCACCA
TACCCTCCCT TGCCAAAGGA CAAGTTGAAT CCACCTACAC CTAGTATTTA CTTGGAAAAT
AAACGTGATG CTTTCTTTCC TCCATTACAT CAATTTTGTA CAAATCCGAA CAACCCTGTT
ACAGTAATAC GTGGCCTTGC TGGAGCTCTT AAGTTAGACC TGGGACTTTT CTCTACTAAA
ACTTTGGTGG AAGCTAACAA TGAACATATG GTAGAAGTGA GGACACAGTT GTTGCAGCCA
GCAGATGAAA ACTGGGATCC CACTGGAACA AAGAAAATCT GGCATTGTGA AAGTAATAGA
TCTCATACTA CAATTGCTAA ATATGCACAG TACCAGGCCT CCTCATTCCA GGAATCATTG
AGAGAAGAAA ATGAAAAAAG AAGTCATCAT AAAGACCACT CAGATAGTGA ATCTACATCG
TCAGATAATT CTGGGAGGAG GAGGAAAGGA CCCTTTAAAA CCATAAAGTT TGGGACCAAT
ATTGACCTAT CTGATGACAA AAAGTGGAAG TTGCAGCTAC ATGAGCTGAC TAAACTTCCT
GCTTTTGTGC GTGTCGTATC AGCAGGAAAT CTTCTAAGCC ATGTTGGTCA TACCATATTG
GGCATGAACA CAGTTCAACT ATACATGAAA GTTCCAGGGA GCAGAACACC AGGTCATCAG
GAAAATAACA ACTTCTGTTC AGTTAACATA AATATTGGCC CAGGTGACTG TGAATGGTTT
GTTGTTCCTG AAGGTTACTG GGGTGTTCTG AATGACTTCT GTGAAAAAAA TAATTTGAAT
TTCCTAATGG GTTCTTGGTG GCCCAATCTT GAAGATCTTT ATGAAGCAAA TGTTCCAGTG
TATAGGTTTA TTCAGCGACC TGGAGATTTG GTCTGGATAA ATGCAGGCAC TGTTCATTGG
GTTCAGGCTA TTGGCTGGTG CAACAACATT GCTTGGAATG TTGGTCCACT TACAGCCTGC
CAGTATAAAT TGGCAGTGGA ACGGTACGAA TGGAACAAAT TGCAAAGTGT GAAGTCAATA
GTACCCATGG TTCATCTTTC CTGGAATATG GCACGAAATA TCAAGGTCTC AGATCCAAAG
CTTTTTGAAA TGATTAAGAT ATTTTCATTG TCTCCGAATT TTAGAGCTGA AAAGTGCCTT
AGAGATCATC TAGTTCAACC TCTCCGTTCA AATGGAGAAC CTGAGCCACT AAGATTCACA
GGAGAGTATT GTCTTCTAAG AACTCTGAAG CAATGTCAGA CATTGAGGGA AGCTCTCATT
GCTGCAGGAA AAGAGATTAT ATGGCATGGG CGGACAAAAG AAGAACCAGC TCATTACTGT
AGCATTTGTG AAGTGGAGGT TTTTGATCTG CTTTTTGTCA CTAATGAGAG TAATTCACGA
AAGACCTACA TAGTACATTG CCAAGATTGT GCACGAAAAA CAAGCGGAAA CTTGGAAAAC
TTTGTGGTGC TAGAACAGTA CAAAATGGAG GACCTGATGC AAGTCTATGA CCAATTTACA
TTAGCTCCTC CATTACCATC CGCCTCATCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542271.1
CDS378..4610
Misc Feature(1)654..740(+)
Misc Feature(2)657..914(+)
Misc Feature(3)693..1580(+)
Misc Feature(4)762..854(+)
Misc Feature(5)765..866(+)
Misc Feature(6)867..959(+)
Misc Feature(7)990..1076(+)
Misc Feature(8)993..1265(+)
Misc Feature(9)1089..1214(+)
Misc Feature(10)1191..1286(+)
Misc Feature(11)1227..1313(+)
Misc Feature(12)1230..1469(+)
Misc Feature(13)1230..1313(+)
Misc Feature(14)1326..1418(+)
Misc Feature(15)1329..1430(+)
Misc Feature(16)1431..1511(+)
Misc Feature(17)3591..3785(+)
Misc Feature(18)3693..4019(+)
Translation

Target ORF information:

RefSeq Version XM_011543969
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543969

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA
TTACAGATGA GACCAACAGG AGTTGCACAG GTACGATCTA CTGGAATTCC TAATGGGCCA
ACAGCTGACT CATCACTGCC TACAAACTCA GTCTCTGGCC AGCAGCCACA GCTTGCTCTG
ACCAGAGTGC CTAGCGTCTC TCAGCCTGGA GTCCGTCCTG CCTGCCCTGG GCAGCCTTTG
GCCAATGGAC CCTTTTCTGC AGGCCATGTT CCCTGTAGCA CATCAAGAAC GCTGGGAAGT
ACAGACACTA TTTTGATAGG CAATAATCAT ATAACAGGAA GTGGAAGTAA TGGAAACGTG
CCTTACCTGC AGCGAAACGC ACTCACTCTA CCTCATAACC GCACAAACCT GACCAGCAGC
GCAGAGGAGC CGTGGAAAAA CCAACTATCT AACTCCACTC AGGGGCTTCA CAAAGGTCAG
AGTTCACATT CGGCAGGTCC TAATGGTGAA CGACCTCTCT CTTCCACTGG GCCTTCCCAG
CATCTCCAGG CAGCTGGCTC TGGTATTCAG AATCAGAACG GACATCCCAC CCTGCCTAGC
AATTCAGTAA CACAGGGGGC TGCTCTCAAT CACCTCTCCT CTCACACTGC TACCTCAGGT
GGACAACAAG GCATTACCTT AACCAAAGAG AGCAAGCCTT CAGGAAACAT ATTGACGGTG
CCTGAAACAA GCAGGCACAC TGGAGAGACA CCTAACAGCA CTGCCAGTGT CGAGGGACTT
CCTAATCATG TCCATCAGAT GACGGCAGAT GCTGTTTGCA GTCCTAGCCA TGGAGATTCT
AAGTCACCAG GTTTACTAAG TTCAGACAAT CCTCAGCTCT CTGCCTTGTT GATGGGAAAA
GCCAATAACA ATGTGGGTAC TGGAACCTGT GACAAAGTCA ATAACATCCA CCCAGCTGTT
CATACAAAGA CTGATAACTC TGTTGCCTCT TCACCATCTT CAGCCATTTC AACAGCAACA
CCTTCTCCAA AATCCACTGA GCAGACAACC ACAAACAGTG TTACCAGCCT TAACAGCCCT
CACAGTGGGC TACACACAAT TAATGGAGAA GGGATGGAAG AATCTCAGAG CCCCATGAAA
ACAGATCTGC TTCTGGTTAA CCACAAACCT AGTCCACAGA TCATACCATC AATGTCTGTG
TCCATATACC CCAGCTCAGC AGAAGTTCTG AAGGCATGCA GGAATCTAGG TAAAAATGGC
TTATCTAACA GTAGCATTTT GTTGGATAAA TGTCCACCTC CAAGACCACC ATCTTCACCA
TACCCTCCCT TGCCAAAGGA CAAGTTGAAT CCACCTACAC CTAGTATTTA CTTGGAAAAT
AAACGTGATG CTTTCTTTCC TCCATTACAT CAATTTTGTA CAAATCCGAA CAACCCTGTT
ACAGTAATAC GTGGCCTTGC TGGAGCTCTT AAGTTAGACC TGGGACTTTT CTCTACTAAA
ACTTTGGTGG AAGCTAACAA TGAACATATG GTAGAAGTGA GGACACAGTT GTTGCAGCCA
GCAGATGAAA ACTGGGATCC CACTGGAACA AAGAAAATCT GGCATTGTGA AAGTAATAGA
TCTCATACTA CAATTGCTAA ATATGCACAG TACCAGGCCT CCTCATTCCA GGAATCATTG
AGAGAAGAAA ATGAAAAAAG AAGTCATCAT AAAGACCACT CAGATAGTGA ATCTACATCG
TCAGATAATT CTGGGAGGAG GAGGAAAGGA CCCTTTAAAA CCATAAAGTT TGGGACCAAT
ATTGACCTAT CTGATGACAA AAAGTGGAAG TTGCAGCTAC ATGAGCTGAC TAAACTTCCT
GCTTTTGTGC GTGTCGTATC AGCAGGAAAT CTTCTAAGCC ATGTTGGTCA TACCATATTG
GGCATGAACA CAGTTCAACT ATACATGAAA GTTCCAGGGA GCAGAACACC AGGTCATCAG
GAAAATAACA ACTTCTGTTC AGTTAACATA AATATTGGCC CAGGTGACTG TGAATGGTTT
GTTGTTCCTG AAGGTTACTG GGGTGTTCTG AATGACTTCT GTGAAAAAAA TAATTTGAAT
TTCCTAATGG GTTCTTGGTG GCCCAATCTT GAAGATCTTT ATGAAGCAAA TGTTCCAGTG
TATAGGTTTA TTCAGCGACC TGGAGATTTG GTCTGGATAA ATGCAGGCAC TGTTCATTGG
GTTCAGGCTA TTGGCTGGTG CAACAACATT GCTTGGAATG TTGGTCCACT TACAGCCTGC
CAGTATAAAT TGGCAGTGGA ACGGTACGAA TGGAACAAAT TGCAAAGTGT GAAGTCAATA
GTACCCATGG TTCATCTTTC CTGGAATATG GCACGAAATA TCAAGGTCTC AGATCCAAAG
CTTTTTGAAA TGATTAAGAT ATTTTCATTG TCTCCGAATT TTAGAGCTGA AAAGTGCCTT
AGAGATCATC TAGTTCAACC TCTCCGTTCA AATGGAGAAC CTGAGCCACT AAGATTCACA
GGAGAGTATT GTCTTCTAAG AACTCTGAAG CAATGTCAGA CATTGAGGGA AGCTCTCATT
GCTGCAGGAA AAGAGATTAT ATGGCATGGG CGGACAAAAG AAGAACCAGC TCATTACTGT
AGCATTTGTG AAGTGGAGGT TTTTGATCTG CTTTTTGTCA CTAATGAGAG TAATTCACGA
AAGACCTACA TAGTACATTG CCAAGATTGT GCACGAAAAA CAAGCGGAAA CTTGGAAAAC
TTTGTGGTGC TAGAACAGTA CAAAATGGAG GACCTGATGC AAGTCTATGA CCAATTTACA
TTAGCTCCTC CATTACCATC CGCCTCATCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61261
Clone ID Related Accession (Same CDS sequence) XM_011543970
Accession Version XM_011543970.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X15
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACCAACAGGA
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGATA
TTTTCATTGT CTCCGAATTT TAGAGCTGAA AAGTGCCTTA GAGATCATCT AGTTCAACCT
CTCCGTTCAA ATGGAGAACC TGAGCCACTA AGATTCACAG GAGAGTATTG TCTTCTAAGA
ACTCTGAAGC AATGTCAGAC ATTGAGGGAA GCTCTCATTG CTGCAGGAAA AGAGATTATA
TGGCATGGGC GGACAAAAGA AGAACCAGCT CATTACTGTA GCATTTGTGA AGTGGAGGTT
TTTGATCTGC TTTTTGTCAC TAATGAGAGT AATTCACGAA AGACCTACAT AGTACATTGC
CAAGATTGTG CACGAAAAAC AAGCGGAAAC TTGGAAAACT TTGTGGTGCT AGAACAGTAC
AAAATGGAGG ACCTGATGCA AGTCTATGAC CAATTTACAT TAGCTCCTCC ATTACCATCC
GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542272.1
CDS378..4589
Misc Feature(1)654..740(+)
Misc Feature(2)657..914(+)
Misc Feature(3)693..1574(+)
Misc Feature(4)762..854(+)
Misc Feature(5)765..866(+)
Misc Feature(6)867..959(+)
Misc Feature(7)990..1076(+)
Misc Feature(8)993..1265(+)
Misc Feature(9)1089..1214(+)
Misc Feature(10)1191..1286(+)
Misc Feature(11)1227..1313(+)
Misc Feature(12)1230..1469(+)
Misc Feature(13)1230..1313(+)
Misc Feature(14)1326..1418(+)
Misc Feature(15)1329..1430(+)
Misc Feature(16)1431..1511(+)
Misc Feature(17)3570..3764(+)
Misc Feature(18)3672..3998(+)
Translation

Target ORF information:

RefSeq Version XM_011543970
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543970

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACCAACAGGA
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGATA
TTTTCATTGT CTCCGAATTT TAGAGCTGAA AAGTGCCTTA GAGATCATCT AGTTCAACCT
CTCCGTTCAA ATGGAGAACC TGAGCCACTA AGATTCACAG GAGAGTATTG TCTTCTAAGA
ACTCTGAAGC AATGTCAGAC ATTGAGGGAA GCTCTCATTG CTGCAGGAAA AGAGATTATA
TGGCATGGGC GGACAAAAGA AGAACCAGCT CATTACTGTA GCATTTGTGA AGTGGAGGTT
TTTGATCTGC TTTTTGTCAC TAATGAGAGT AATTCACGAA AGACCTACAT AGTACATTGC
CAAGATTGTG CACGAAAAAC AAGCGGAAAC TTGGAAAACT TTGTGGTGCT AGAACAGTAC
AAAATGGAGG ACCTGATGCA AGTCTATGAC CAATTTACAT TAGCTCCTCC ATTACCATCC
GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61262
Clone ID Related Accession (Same CDS sequence) XM_011543971
Accession Version XM_011543971.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X16
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA
CAGATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT
TTTGAAATGA TTAAGTATTG TCTTCTAAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCT
CATTACTGTA GCATTTGTGA AGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT
AATTCACGAA AGACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGCGGAAAC
TTGGAAAACT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542273.1
CDS378..4559
Misc Feature(1)654..740(+)
Misc Feature(2)657..971(+)
Misc Feature(3)810..1637(+)
Misc Feature(4)819..911(+)
Misc Feature(5)822..923(+)
Misc Feature(6)924..1016(+)
Misc Feature(7)1047..1133(+)
Misc Feature(8)1050..1322(+)
Misc Feature(9)1146..1271(+)
Misc Feature(10)1248..1343(+)
Misc Feature(11)1284..1370(+)
Misc Feature(12)1287..1526(+)
Misc Feature(13)1287..1370(+)
Misc Feature(14)1383..1475(+)
Misc Feature(15)1386..1487(+)
Misc Feature(16)1488..1568(+)
Misc Feature(17)3648..3842(+)
Misc Feature(18)3750..4076(+)
Translation

Target ORF information:

RefSeq Version XM_011543971
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X17, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543971

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA
CAGATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT
TTTGAAATGA TTAAGTATTG TCTTCTAAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCT
CATTACTGTA GCATTTGTGA AGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT
AATTCACGAA AGACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGCGGAAAC
TTGGAAAACT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61263
Clone ID Related Accession (Same CDS sequence) XM_011543972
Accession Version XM_011543972.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X17
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT
TCATGGTGTT TGACACCACA GAAATTACAG CATTTGGAAC AGCTCCGCGC AAATAGAAAT
AATTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG
CAACAACACC AAATGAGACC AACAGGAGTT GCACAGGTAC GATCTACTGG AATTCCTAAT
GGGCCAACAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT
GCTCTGACCA GAGTGCCTAG CGTCTCTCAG CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG
CCTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG
GGAAGTACAG ACACTATTTT GATAGGCAAT AATCATATAA CAGGAAGTGG AAGTAATGGA
AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA
GGTCAGAGTT CACATTCGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAACGGACA TCCCACCCTG
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACATATTG
ACGGTGCCTG AAACAAGCAG GCACACTGGA GAGACACCTA ACAGCACTGC CAGTGTCGAG
GGACTTCCTA ATCATGTCCA TCAGATGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG
GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA
GCTGTTCATA CAAAGACTGA TAACTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCAACA
GCAACACCTT CTCCAAAATC CACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC
AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGGA TGGAAGAATC TCAGAGCCCC
ATGAAAACAG ATCTGCTTCT GGTTAACCAC AAACCTAGTC CACAGATCAT ACCATCAATG
TCTGTGTCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA TCTAGGTAAA
AATGGCTTAT CTAACAGTAG CATTTTGTTG GATAAATGTC CACCTCCAAG ACCACCATCT
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTACTTG
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA TCCGAACAAC
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TAGACCTGGG ACTTTTCTCT
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTG
CAGCCAGCAG ATGAAAACTG GGATCCCACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT
AATAGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC ATTCCAGGAA
TCATTGAGAG AAGAAAATGA AAAAAGAAGT CATCATAAAG ACCACTCAGA TAGTGAATCT
ACATCGTCAG ATAATTCTGG GAGGAGGAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG
ACCAATATTG ACCTATCTGA TGACAAAAAG TGGAAGTTGC AGCTACATGA GCTGACTAAA
CTTCCTGCTT TTGTGCGTGT CGTATCAGCA GGAAATCTTC TAAGCCATGT TGGTCATACC
ATATTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGGAGCAG AACACCAGGT
CATCAGGAAA ATAACAACTT CTGTTCAGTT AACATAAATA TTGGCCCAGG TGACTGTGAA
TGGTTTGTTG TTCCTGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT
TTGAATTTCC TAATGGGTTC TTGGTGGCCC AATCTTGAAG ATCTTTATGA AGCAAATGTT
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTT
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA
GCCTGCCAGT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT
CCAAAGCTTT TTGAAATGAT TAAGATATTT TCATTGTCTC CGAATTTTAG AGCTGAAAAG
TGCCTTAGAG ATCATCTAGT TCAACCTCTC CGTTCAAATG GAGAACCTGA GCCACTAAGA
TTCACAGGAG AGTATTGTCT TCTAAGAACT CTGAAGCAAT GTCAGACATT GAGGGAAGCT
CTCATTGCTG CAGGAAAAGA GATTATATGG CATGGGCGGA CAAAAGAAGA ACCAGCTCAT
TACTGTAGCA TTTGTGAAGT GGAGGTTTTT GATCTGCTTT TTGTCACTAA TGAGAGTAAT
TCACGAAAGA CCTACATAGT ACATTGCCAA GATTGTGCAC GAAAAACAAG CGGAAACTTG
GAAAACTTTG TGGTGCTAGA ACAGTACAAA ATGGAGGACC TGATGCAAGT CTATGACCAA
TTTACATTAG CTCCTCCATT ACCATCCGCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542274.1
CDS378..4556
Misc Feature(1)651..860(+)
Misc Feature(2)654..740(+)
Misc Feature(3)657..914(+)
Misc Feature(4)759..959(+)
Misc Feature(5)762..854(+)
Misc Feature(6)765..866(+)
Misc Feature(7)867..959(+)
Misc Feature(8)990..1223(+)
Misc Feature(9)990..1076(+)
Misc Feature(10)993..1265(+)
Misc Feature(11)1089..1214(+)
Misc Feature(12)1191..1286(+)
Misc Feature(13)1221..1421(+)
Misc Feature(14)1227..1313(+)
Misc Feature(15)1230..1469(+)
Misc Feature(16)1230..1313(+)
Misc Feature(17)1326..1418(+)
Misc Feature(18)1329..1430(+)
Misc Feature(19)1431..1511(+)
Misc Feature(20)3537..3731(+)
Misc Feature(21)3639..3965(+)
Translation

Target ORF information:

RefSeq Version XM_011543972
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X18, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543972

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT
TCATGGTGTT TGACACCACA GAAATTACAG CATTTGGAAC AGCTCCGCGC AAATAGAAAT
AATTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG
CAACAACACC AAATGAGACC AACAGGAGTT GCACAGGTAC GATCTACTGG AATTCCTAAT
GGGCCAACAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT
GCTCTGACCA GAGTGCCTAG CGTCTCTCAG CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG
CCTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG
GGAAGTACAG ACACTATTTT GATAGGCAAT AATCATATAA CAGGAAGTGG AAGTAATGGA
AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA
GGTCAGAGTT CACATTCGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAACGGACA TCCCACCCTG
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACATATTG
ACGGTGCCTG AAACAAGCAG GCACACTGGA GAGACACCTA ACAGCACTGC CAGTGTCGAG
GGACTTCCTA ATCATGTCCA TCAGATGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG
GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA
GCTGTTCATA CAAAGACTGA TAACTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCAACA
GCAACACCTT CTCCAAAATC CACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC
AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGGA TGGAAGAATC TCAGAGCCCC
ATGAAAACAG ATCTGCTTCT GGTTAACCAC AAACCTAGTC CACAGATCAT ACCATCAATG
TCTGTGTCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA TCTAGGTAAA
AATGGCTTAT CTAACAGTAG CATTTTGTTG GATAAATGTC CACCTCCAAG ACCACCATCT
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTACTTG
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA TCCGAACAAC
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TAGACCTGGG ACTTTTCTCT
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTG
CAGCCAGCAG ATGAAAACTG GGATCCCACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT
AATAGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC ATTCCAGGAA
TCATTGAGAG AAGAAAATGA AAAAAGAAGT CATCATAAAG ACCACTCAGA TAGTGAATCT
ACATCGTCAG ATAATTCTGG GAGGAGGAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG
ACCAATATTG ACCTATCTGA TGACAAAAAG TGGAAGTTGC AGCTACATGA GCTGACTAAA
CTTCCTGCTT TTGTGCGTGT CGTATCAGCA GGAAATCTTC TAAGCCATGT TGGTCATACC
ATATTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGGAGCAG AACACCAGGT
CATCAGGAAA ATAACAACTT CTGTTCAGTT AACATAAATA TTGGCCCAGG TGACTGTGAA
TGGTTTGTTG TTCCTGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT
TTGAATTTCC TAATGGGTTC TTGGTGGCCC AATCTTGAAG ATCTTTATGA AGCAAATGTT
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTT
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA
GCCTGCCAGT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT
CCAAAGCTTT TTGAAATGAT TAAGATATTT TCATTGTCTC CGAATTTTAG AGCTGAAAAG
TGCCTTAGAG ATCATCTAGT TCAACCTCTC CGTTCAAATG GAGAACCTGA GCCACTAAGA
TTCACAGGAG AGTATTGTCT TCTAAGAACT CTGAAGCAAT GTCAGACATT GAGGGAAGCT
CTCATTGCTG CAGGAAAAGA GATTATATGG CATGGGCGGA CAAAAGAAGA ACCAGCTCAT
TACTGTAGCA TTTGTGAAGT GGAGGTTTTT GATCTGCTTT TTGTCACTAA TGAGAGTAAT
TCACGAAAGA CCTACATAGT ACATTGCCAA GATTGTGCAC GAAAAACAAG CGGAAACTTG
GAAAACTTTG TGGTGCTAGA ACAGTACAAA ATGGAGGACC TGATGCAAGT CTATGACCAA
TTTACATTAG CTCCTCCATT ACCATCCGCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61264
Clone ID Related Accession (Same CDS sequence) XM_011543973
Accession Version XM_011543973.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4128bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X18
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GAATACTTCT
GACAATTGGA GTGGTGGACA TGCTGTGTCA CATCCTCCAG TACAGCAACA AGCTCATTCA
TGGTGTTTGA CACCACAGAA ATTACAGCAT TTGGAACAGC TCCGCGCAAA TAGAAATAAT
TTAAATCCAG CACAGAAACT GATGCTGGAA CAGCTGGAAA GTCAGTTTGT CTTAATGCAA
CAACACCAAA TGAGACCAAC AGGAGTTGCA CAGGTACGAT CTACTGGAAT TCCTAATGGG
CCAACAGCTG ACTCATCACT GCCTACAAAC TCAGTCTCTG GCCAGCAGCC ACAGCTTGCT
CTGACCAGAG TGCCTAGCGT CTCTCAGCCT GGAGTCCGTC CTGCCTGCCC TGGGCAGCCT
TTGGCCAATG GACCCTTTTC TGCAGGCCAT GTTCCCTGTA GCACATCAAG AACGCTGGGA
AGTACAGACA CTATTTTGAT AGGCAATAAT CATATAACAG GAAGTGGAAG TAATGGAAAC
GTGCCTTACC TGCAGCGAAA CGCACTCACT CTACCTCATA ACCGCACAAA CCTGACCAGC
AGCGCAGAGG AGCCGTGGAA AAACCAACTA TCTAACTCCA CTCAGGGGCT TCACAAAGGT
CAGAGTTCAC ATTCGGCAGG TCCTAATGGT GAACGACCTC TCTCTTCCAC TGGGCCTTCC
CAGCATCTCC AGGCAGCTGG CTCTGGTATT CAGAATCAGA ACGGACATCC CACCCTGCCT
AGCAATTCAG TAACACAGGG GGCTGCTCTC AATCACCTCT CCTCTCACAC TGCTACCTCA
GGTGGACAAC AAGGCATTAC CTTAACCAAA GAGAGCAAGC CTTCAGGAAA CATATTGACG
GTGCCTGAAA CAAGCAGGCA CACTGGAGAG ACACCTAACA GCACTGCCAG TGTCGAGGGA
CTTCCTAATC ATGTCCATCA GATGACGGCA GATGCTGTTT GCAGTCCTAG CCATGGAGAT
TCTAAGTCAC CAGGTTTACT AAGTTCAGAC AATCCTCAGC TCTCTGCCTT GTTGATGGGA
AAAGCCAATA ACAATGTGGG TACTGGAACC TGTGACAAAG TCAATAACAT CCACCCAGCT
GTTCATACAA AGACTGATAA CTCTGTTGCC TCTTCACCAT CTTCAGCCAT TTCAACAGCA
ACACCTTCTC CAAAATCCAC TGAGCAGACA ACCACAAACA GTGTTACCAG CCTTAACAGC
CCTCACAGTG GGCTACACAC AATTAATGGA GAAGGGATGG AAGAATCTCA GAGCCCCATG
AAAACAGATC TGCTTCTGGT TAACCACAAA CCTAGTCCAC AGATCATACC ATCAATGTCT
GTGTCCATAT ACCCCAGCTC AGCAGAAGTT CTGAAGGCAT GCAGGAATCT AGGTAAAAAT
GGCTTATCTA ACAGTAGCAT TTTGTTGGAT AAATGTCCAC CTCCAAGACC ACCATCTTCA
CCATACCCTC CCTTGCCAAA GGACAAGTTG AATCCACCTA CACCTAGTAT TTACTTGGAA
AATAAACGTG ATGCTTTCTT TCCTCCATTA CATCAATTTT GTACAAATCC GAACAACCCT
GTTACAGTAA TACGTGGCCT TGCTGGAGCT CTTAAGTTAG ACCTGGGACT TTTCTCTACT
AAAACTTTGG TGGAAGCTAA CAATGAACAT ATGGTAGAAG TGAGGACACA GTTGTTGCAG
CCAGCAGATG AAAACTGGGA TCCCACTGGA ACAAAGAAAA TCTGGCATTG TGAAAGTAAT
AGATCTCATA CTACAATTGC TAAATATGCA CAGTACCAGG CCTCCTCATT CCAGGAATCA
TTGAGAGAAG AAAATGAAAA AAGAAGTCAT CATAAAGACC ACTCAGATAG TGAATCTACA
TCGTCAGATA ATTCTGGGAG GAGGAGGAAA GGACCCTTTA AAACCATAAA GTTTGGGACC
AATATTGACC TATCTGATGA CAAAAAGTGG AAGTTGCAGC TACATGAGCT GACTAAACTT
CCTGCTTTTG TGCGTGTCGT ATCAGCAGGA AATCTTCTAA GCCATGTTGG TCATACCATA
TTGGGCATGA ACACAGTTCA ACTATACATG AAAGTTCCAG GGAGCAGAAC ACCAGGTCAT
CAGGAAAATA ACAACTTCTG TTCAGTTAAC ATAAATATTG GCCCAGGTGA CTGTGAATGG
TTTGTTGTTC CTGAAGGTTA CTGGGGTGTT CTGAATGACT TCTGTGAAAA AAATAATTTG
AATTTCCTAA TGGGTTCTTG GTGGCCCAAT CTTGAAGATC TTTATGAAGC AAATGTTCCA
GTGTATAGGT TTATTCAGCG ACCTGGAGAT TTGGTCTGGA TAAATGCAGG CACTGTTCAT
TGGGTTCAGG CTATTGGCTG GTGCAACAAC ATTGCTTGGA ATGTTGGTCC ACTTACAGCC
TGCCAGTATA AATTGGCAGT GGAACGGTAC GAATGGAACA AATTGCAAAG TGTGAAGTCA
ATAGTACCCA TGGTTCATCT TTCCTGGAAT ATGGCACGAA ATATCAAGGT CTCAGATCCA
AAGCTTTTTG AAATGATTAA GTATTGTCTT CTAAGAACTC TGAAGCAATG TCAGACATTG
AGGGAAGCTC TCATTGCTGC AGGAAAAGAG ATTATATGGC ATGGGCGGAC AAAAGAAGAA
CCAGCTCATT ACTGTAGCAT TTGTGAAGTG GAGGTTTTTG ATCTGCTTTT TGTCACTAAT
GAGAGTAATT CACGAAAGAC CTACATAGTA CATTGCCAAG ATTGTGCACG AAAAACAAGC
GGAAACTTGG AAAACTTTGT GGTGCTAGAA CAGTACAAAA TGGAGGACCT GATGCAAGTC
TATGACCAAT TTACATTAGC TCCTCCATTA CCATCCGCCT CATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542275.1
CDS378..4505
Misc Feature(1)654..740(+)
Misc Feature(2)657..971(+)
Misc Feature(3)819..1016(+)
Misc Feature(4)819..911(+)
Misc Feature(5)822..923(+)
Misc Feature(6)924..1016(+)
Misc Feature(7)1047..1280(+)
Misc Feature(8)1047..1133(+)
Misc Feature(9)1050..1322(+)
Misc Feature(10)1146..1271(+)
Misc Feature(11)1248..1343(+)
Misc Feature(12)1278..1478(+)
Misc Feature(13)1284..1370(+)
Misc Feature(14)1287..1526(+)
Misc Feature(15)1287..1370(+)
Misc Feature(16)1383..1475(+)
Misc Feature(17)1386..1487(+)
Misc Feature(18)1488..1568(+)
Misc Feature(19)3594..3788(+)
Misc Feature(20)3696..4022(+)
Translation

Target ORF information:

RefSeq Version XM_011543973
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X19, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543973

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GAATACTTCT
GACAATTGGA GTGGTGGACA TGCTGTGTCA CATCCTCCAG TACAGCAACA AGCTCATTCA
TGGTGTTTGA CACCACAGAA ATTACAGCAT TTGGAACAGC TCCGCGCAAA TAGAAATAAT
TTAAATCCAG CACAGAAACT GATGCTGGAA CAGCTGGAAA GTCAGTTTGT CTTAATGCAA
CAACACCAAA TGAGACCAAC AGGAGTTGCA CAGGTACGAT CTACTGGAAT TCCTAATGGG
CCAACAGCTG ACTCATCACT GCCTACAAAC TCAGTCTCTG GCCAGCAGCC ACAGCTTGCT
CTGACCAGAG TGCCTAGCGT CTCTCAGCCT GGAGTCCGTC CTGCCTGCCC TGGGCAGCCT
TTGGCCAATG GACCCTTTTC TGCAGGCCAT GTTCCCTGTA GCACATCAAG AACGCTGGGA
AGTACAGACA CTATTTTGAT AGGCAATAAT CATATAACAG GAAGTGGAAG TAATGGAAAC
GTGCCTTACC TGCAGCGAAA CGCACTCACT CTACCTCATA ACCGCACAAA CCTGACCAGC
AGCGCAGAGG AGCCGTGGAA AAACCAACTA TCTAACTCCA CTCAGGGGCT TCACAAAGGT
CAGAGTTCAC ATTCGGCAGG TCCTAATGGT GAACGACCTC TCTCTTCCAC TGGGCCTTCC
CAGCATCTCC AGGCAGCTGG CTCTGGTATT CAGAATCAGA ACGGACATCC CACCCTGCCT
AGCAATTCAG TAACACAGGG GGCTGCTCTC AATCACCTCT CCTCTCACAC TGCTACCTCA
GGTGGACAAC AAGGCATTAC CTTAACCAAA GAGAGCAAGC CTTCAGGAAA CATATTGACG
GTGCCTGAAA CAAGCAGGCA CACTGGAGAG ACACCTAACA GCACTGCCAG TGTCGAGGGA
CTTCCTAATC ATGTCCATCA GATGACGGCA GATGCTGTTT GCAGTCCTAG CCATGGAGAT
TCTAAGTCAC CAGGTTTACT AAGTTCAGAC AATCCTCAGC TCTCTGCCTT GTTGATGGGA
AAAGCCAATA ACAATGTGGG TACTGGAACC TGTGACAAAG TCAATAACAT CCACCCAGCT
GTTCATACAA AGACTGATAA CTCTGTTGCC TCTTCACCAT CTTCAGCCAT TTCAACAGCA
ACACCTTCTC CAAAATCCAC TGAGCAGACA ACCACAAACA GTGTTACCAG CCTTAACAGC
CCTCACAGTG GGCTACACAC AATTAATGGA GAAGGGATGG AAGAATCTCA GAGCCCCATG
AAAACAGATC TGCTTCTGGT TAACCACAAA CCTAGTCCAC AGATCATACC ATCAATGTCT
GTGTCCATAT ACCCCAGCTC AGCAGAAGTT CTGAAGGCAT GCAGGAATCT AGGTAAAAAT
GGCTTATCTA ACAGTAGCAT TTTGTTGGAT AAATGTCCAC CTCCAAGACC ACCATCTTCA
CCATACCCTC CCTTGCCAAA GGACAAGTTG AATCCACCTA CACCTAGTAT TTACTTGGAA
AATAAACGTG ATGCTTTCTT TCCTCCATTA CATCAATTTT GTACAAATCC GAACAACCCT
GTTACAGTAA TACGTGGCCT TGCTGGAGCT CTTAAGTTAG ACCTGGGACT TTTCTCTACT
AAAACTTTGG TGGAAGCTAA CAATGAACAT ATGGTAGAAG TGAGGACACA GTTGTTGCAG
CCAGCAGATG AAAACTGGGA TCCCACTGGA ACAAAGAAAA TCTGGCATTG TGAAAGTAAT
AGATCTCATA CTACAATTGC TAAATATGCA CAGTACCAGG CCTCCTCATT CCAGGAATCA
TTGAGAGAAG AAAATGAAAA AAGAAGTCAT CATAAAGACC ACTCAGATAG TGAATCTACA
TCGTCAGATA ATTCTGGGAG GAGGAGGAAA GGACCCTTTA AAACCATAAA GTTTGGGACC
AATATTGACC TATCTGATGA CAAAAAGTGG AAGTTGCAGC TACATGAGCT GACTAAACTT
CCTGCTTTTG TGCGTGTCGT ATCAGCAGGA AATCTTCTAA GCCATGTTGG TCATACCATA
TTGGGCATGA ACACAGTTCA ACTATACATG AAAGTTCCAG GGAGCAGAAC ACCAGGTCAT
CAGGAAAATA ACAACTTCTG TTCAGTTAAC ATAAATATTG GCCCAGGTGA CTGTGAATGG
TTTGTTGTTC CTGAAGGTTA CTGGGGTGTT CTGAATGACT TCTGTGAAAA AAATAATTTG
AATTTCCTAA TGGGTTCTTG GTGGCCCAAT CTTGAAGATC TTTATGAAGC AAATGTTCCA
GTGTATAGGT TTATTCAGCG ACCTGGAGAT TTGGTCTGGA TAAATGCAGG CACTGTTCAT
TGGGTTCAGG CTATTGGCTG GTGCAACAAC ATTGCTTGGA ATGTTGGTCC ACTTACAGCC
TGCCAGTATA AATTGGCAGT GGAACGGTAC GAATGGAACA AATTGCAAAG TGTGAAGTCA
ATAGTACCCA TGGTTCATCT TTCCTGGAAT ATGGCACGAA ATATCAAGGT CTCAGATCCA
AAGCTTTTTG AAATGATTAA GTATTGTCTT CTAAGAACTC TGAAGCAATG TCAGACATTG
AGGGAAGCTC TCATTGCTGC AGGAAAAGAG ATTATATGGC ATGGGCGGAC AAAAGAAGAA
CCAGCTCATT ACTGTAGCAT TTGTGAAGTG GAGGTTTTTG ATCTGCTTTT TGTCACTAAT
GAGAGTAATT CACGAAAGAC CTACATAGTA CATTGCCAAG ATTGTGCACG AAAAACAAGC
GGAAACTTGG AAAACTTTGT GGTGCTAGAA CAGTACAAAA TGGAGGACCT GATGCAAGTC
TATGACCAAT TTACATTAGC TCCTCCATTA CCATCCGCCT CATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54024
Clone ID Related Accession (Same CDS sequence) XM_005272659
Accession Version XM_005272659.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4104bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X19
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838078. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACCAACAGGA
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT
TGTCTTCTAA GAACTCTGAA GCAATGTCAG ACATTGAGGG AAGCTCTCAT TGCTGCAGGA
AAAGAGATTA TATGGCATGG GCGGACAAAA GAAGAACCAG CTCATTACTG TAGCATTTGT
GAAGTGGAGG TTTTTGATCT GCTTTTTGTC ACTAATGAGA GTAATTCACG AAAGACCTAC
ATAGTACATT GCCAAGATTG TGCACGAAAA ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG
CTAGAACAGT ACAAAATGGA GGACCTGATG CAAGTCTATG ACCAATTTAC ATTAGCTCCT
CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005272716.1
CDS378..4481
Misc Feature(1)654..740(+)
Misc Feature(2)657..914(+)
Misc Feature(3)693..1574(+)
Misc Feature(4)762..854(+)
Misc Feature(5)765..866(+)
Misc Feature(6)867..959(+)
Misc Feature(7)990..1076(+)
Misc Feature(8)993..1265(+)
Misc Feature(9)1089..1214(+)
Misc Feature(10)1191..1286(+)
Misc Feature(11)1227..1313(+)
Misc Feature(12)1230..1469(+)
Misc Feature(13)1230..1313(+)
Misc Feature(14)1326..1418(+)
Misc Feature(15)1329..1430(+)
Misc Feature(16)1431..1511(+)
Misc Feature(17)3570..3764(+)
Misc Feature(18)3672..3998(+)
Translation

Target ORF information:

RefSeq Version XM_005272659
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X20, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005272659

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACCAACAGGA
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT
TGTCTTCTAA GAACTCTGAA GCAATGTCAG ACATTGAGGG AAGCTCTCAT TGCTGCAGGA
AAAGAGATTA TATGGCATGG GCGGACAAAA GAAGAACCAG CTCATTACTG TAGCATTTGT
GAAGTGGAGG TTTTTGATCT GCTTTTTGTC ACTAATGAGA GTAATTCACG AAAGACCTAC
ATAGTACATT GCCAAGATTG TGCACGAAAA ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG
CTAGAACAGT ACAAAATGGA GGACCTGATG CAAGTCTATG ACCAATTTAC ATTAGCTCCT
CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61265
Clone ID Related Accession (Same CDS sequence) XM_011543974
Accession Version XM_011543974.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X20
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542276.1
CDS378..4259
Misc Feature(1)654..740(+)
Misc Feature(2)657..914(+)
Misc Feature(3)693..1574(+)
Misc Feature(4)762..854(+)
Misc Feature(5)765..866(+)
Misc Feature(6)867..959(+)
Misc Feature(7)990..1076(+)
Misc Feature(8)993..1265(+)
Misc Feature(9)1089..1214(+)
Misc Feature(10)1191..1286(+)
Misc Feature(11)1227..1313(+)
Misc Feature(12)1230..1469(+)
Misc Feature(13)1230..1313(+)
Misc Feature(14)1326..1418(+)
Misc Feature(15)1329..1430(+)
Misc Feature(16)1431..1511(+)
Misc Feature(17)3672..3866(+)
Misc Feature(18)3774..4100(+)
Translation

Target ORF information:

RefSeq Version XM_011543974
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X21, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543974

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61266
Clone ID Related Accession (Same CDS sequence) XM_011543975
Accession Version XM_011543975.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X21
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCATCACA CTGTAGATCT CCTGGGAGAT AAAGCCACCA AGGAAAGCTA TGCTATTCAG 
TATCTCCAAA AGTCCTTGGA AGCAGATCCT AATTCTGGCC AGTCCTGGTA TTTCCTCGGA
AGGTGCTATT CAAGTATTGG GAAAGTTCAG GATGCCTTTA TATCTTACAG GCAGTCTATT
GATAAATCAG AAGCAAGTGC AGATACATGG TGTTCAATAG GTGTGCTATA TCAGCAGCAA
AATCAGCCCA TGGATGCTTT ACAGGCCTAT ATTTGTGCTG TACAATTGGA CCATGGCCAT
GCTGCAGCCT GGATGGACCT AGGCACTCTC TATGAATCCT GCAACCAGCC TCAGGATGCC
ATTAAATGCT ACTTAAATGC AACTAGAAGC AAAAGTTGTA GTAATACCTC TGCACTTGCA
GCACGAATTA AGTATTTACA GGCTCAGTTG TGTAACCTTC CACAAGGTAG TCTACAGAAT
AAAACTAAAT TACTTCCTAG TATTGAGGAG GCGTGGAGCC TACCAATTCC CGCAGAGCTT
ACCTCCAGGC AGGGTGCCAT GAACACAGCA CAGCAGGCAT GTAAACCTCA TCATCCAAAT
ACTGAACCTG TATTAGGCCT CAGTCAAACA CCAATTTCAC AGCAATCCTT GCCACTACAC
ATGATTCCTT CTAGCCAAGT AGATGACCTG TCCAGTCCTG CCAAGAGGAA AAGAACATCT
AGTCCAACAA AGAATACTTC TGACAATTGG AGTGGTGGAC ATGCTGTGTC ACATCCTCCA
GTACAGCAAC AAGCTCATTC ATGGTGTTTG ACACCACAGA AATTACAGCA TTTGGAACAG
CTCCGCGCAA ATAGAAATAA TTTAAATCCA GCACAGAAAC TGATGCTGGA ACAGCTGGAA
AGTCAGTTTG TCTTAATGCA ACAACACCAA ATGAGACCAA CAGGAGTTGC ACAGGTACGA
TCTACTGGAA TTCCTAATGG GCCAACAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT
GGCCAGCAGC CACAGCTTGC TCTGACCAGA GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT
CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT GGACCCTTTT CTGCAGGCCA TGTTCCCTGT
AGCACATCAA GAACGCTGGG AAGTACAGAC ACTATTTTGA TAGGCAATAA TCATATAACA
GGAAGTGGAA GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT
AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC
ACTCAGGGGC TTCACAAAGG TCAGAGTTCA CATTCGGCAG GTCCTAATGG TGAACGACCT
CTCTCTTCCA CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG
AACGGACATC CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC
TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG
CCTTCAGGAA ACATATTGAC GGTGCCTGAA ACAAGCAGGC ACACTGGAGA GACACCTAAC
AGCACTGCCA GTGTCGAGGG ACTTCCTAAT CATGTCCATC AGATGACGGC AGATGCTGTT
TGCAGTCCTA GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG
CTCTCTGCCT TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA
GTCAATAACA TCCACCCAGC TGTTCATACA AAGACTGATA ACTCTGTTGC CTCTTCACCA
TCTTCAGCCA TTTCAACAGC AACACCTTCT CCAAAATCCA CTGAGCAGAC AACCACAAAC
AGTGTTACCA GCCTTAACAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGGATG
GAAGAATCTC AGAGCCCCAT GAAAACAGAT CTGCTTCTGG TTAACCACAA ACCTAGTCCA
CAGATCATAC CATCAATGTC TGTGTCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA
TGCAGGAATC TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTGTTGGA TAAATGTCCA
CCTCCAAGAC CACCATCTTC ACCATACCCT CCCTTGCCAA AGGACAAGTT GAATCCACCT
ACACCTAGTA TTTACTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACATCAATTT
TGTACAAATC CGAACAACCC TGTTACAGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTA
GACCTGGGAC TTTTCTCTAC TAAAACTTTG GTGGAAGCTA ACAATGAACA TATGGTAGAA
GTGAGGACAC AGTTGTTGCA GCCAGCAGAT GAAAACTGGG ATCCCACTGG AACAAAGAAA
ATCTGGCATT GTGAAAGTAA TAGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG
GCCTCCTCAT TCCAGGAATC ATTGAGAGAA GAAAATGAAA AAAGAAGTCA TCATAAAGAC
CACTCAGATA GTGAATCTAC ATCGTCAGAT AATTCTGGGA GGAGGAGGAA AGGACCCTTT
AAAACCATAA AGTTTGGGAC CAATATTGAC CTATCTGATG ACAAAAAGTG GAAGTTGCAG
CTACATGAGC TGACTAAACT TCCTGCTTTT GTGCGTGTCG TATCAGCAGG AAATCTTCTA
AGCCATGTTG GTCATACCAT ATTGGGCATG AACACAGTTC AACTATACAT GAAAGTTCCA
GGGAGCAGAA CACCAGGTCA TCAGGAAAAT AACAACTTCT GTTCAGTTAA CATAAATATT
GGCCCAGGTG ACTGTGAATG GTTTGTTGTT CCTGAAGGTT ACTGGGGTGT TCTGAATGAC
TTCTGTGAAA AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT
CTTTATGAAG CAAATGTTCC AGTGTATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG
ATAAATGCAG GCACTGTTCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG
AATGTTGGTC CACTTACAGC CTGCCAGTAT AAATTGGCAG TGGAACGGTA CGAATGGAAC
AAATTGCAAA GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA
AATATCAAGG TCTCAGATCC AAAGCTTTTT GAAATGATTA AGATATTTTC ATTGTCTCCG
AATTTTAGAG CTGAAAAGTG CCTTAGAGAT CATCTAGTTC AACCTCTCCG TTCAAATGGA
GAACCTGAGC CACTAAGATT CACAGGAGAG TATTGTCTTC TAAGAACTCT GAAGCAATGT
CAGACATTGA GGGAAGCTCT CATTGCTGCA GGAAAAGAGA TTATATGGCA TGGGCGGACA
AAAGAAGAAC CAGCTCATTA CTGTAGCATT TGTGAAGTGG AGGTTTTTGA TCTGCTTTTT
GTCACTAATG AGAGTAATTC ACGAAAGACC TACATAGTAC ATTGCCAAGA TTGTGCACGA
AAAACAAGCG GAAACTTGGA AAACTTTGTG GTGCTAGAAC AGTACAAAAT GGAGGACCTG
ATGCAAGTCT ATGACCAATT TACATTAGCT CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542277.1
CDS301..4017
Misc Feature(1)361..456(+)
Misc Feature(2)391..591(+)
Misc Feature(3)400..639(+)
Misc Feature(4)400..483(+)
Misc Feature(5)496..588(+)
Misc Feature(6)499..600(+)
Misc Feature(7)601..681(+)
Misc Feature(8)2998..3192(+)
Misc Feature(9)3100..3426(+)
Translation

Target ORF information:

RefSeq Version XM_011543975
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X22, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543975

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCATCACA CTGTAGATCT CCTGGGAGAT AAAGCCACCA AGGAAAGCTA TGCTATTCAG 
TATCTCCAAA AGTCCTTGGA AGCAGATCCT AATTCTGGCC AGTCCTGGTA TTTCCTCGGA
AGGTGCTATT CAAGTATTGG GAAAGTTCAG GATGCCTTTA TATCTTACAG GCAGTCTATT
GATAAATCAG AAGCAAGTGC AGATACATGG TGTTCAATAG GTGTGCTATA TCAGCAGCAA
AATCAGCCCA TGGATGCTTT ACAGGCCTAT ATTTGTGCTG TACAATTGGA CCATGGCCAT
GCTGCAGCCT GGATGGACCT AGGCACTCTC TATGAATCCT GCAACCAGCC TCAGGATGCC
ATTAAATGCT ACTTAAATGC AACTAGAAGC AAAAGTTGTA GTAATACCTC TGCACTTGCA
GCACGAATTA AGTATTTACA GGCTCAGTTG TGTAACCTTC CACAAGGTAG TCTACAGAAT
AAAACTAAAT TACTTCCTAG TATTGAGGAG GCGTGGAGCC TACCAATTCC CGCAGAGCTT
ACCTCCAGGC AGGGTGCCAT GAACACAGCA CAGCAGGCAT GTAAACCTCA TCATCCAAAT
ACTGAACCTG TATTAGGCCT CAGTCAAACA CCAATTTCAC AGCAATCCTT GCCACTACAC
ATGATTCCTT CTAGCCAAGT AGATGACCTG TCCAGTCCTG CCAAGAGGAA AAGAACATCT
AGTCCAACAA AGAATACTTC TGACAATTGG AGTGGTGGAC ATGCTGTGTC ACATCCTCCA
GTACAGCAAC AAGCTCATTC ATGGTGTTTG ACACCACAGA AATTACAGCA TTTGGAACAG
CTCCGCGCAA ATAGAAATAA TTTAAATCCA GCACAGAAAC TGATGCTGGA ACAGCTGGAA
AGTCAGTTTG TCTTAATGCA ACAACACCAA ATGAGACCAA CAGGAGTTGC ACAGGTACGA
TCTACTGGAA TTCCTAATGG GCCAACAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT
GGCCAGCAGC CACAGCTTGC TCTGACCAGA GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT
CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT GGACCCTTTT CTGCAGGCCA TGTTCCCTGT
AGCACATCAA GAACGCTGGG AAGTACAGAC ACTATTTTGA TAGGCAATAA TCATATAACA
GGAAGTGGAA GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT
AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC
ACTCAGGGGC TTCACAAAGG TCAGAGTTCA CATTCGGCAG GTCCTAATGG TGAACGACCT
CTCTCTTCCA CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG
AACGGACATC CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC
TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG
CCTTCAGGAA ACATATTGAC GGTGCCTGAA ACAAGCAGGC ACACTGGAGA GACACCTAAC
AGCACTGCCA GTGTCGAGGG ACTTCCTAAT CATGTCCATC AGATGACGGC AGATGCTGTT
TGCAGTCCTA GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG
CTCTCTGCCT TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA
GTCAATAACA TCCACCCAGC TGTTCATACA AAGACTGATA ACTCTGTTGC CTCTTCACCA
TCTTCAGCCA TTTCAACAGC AACACCTTCT CCAAAATCCA CTGAGCAGAC AACCACAAAC
AGTGTTACCA GCCTTAACAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGGATG
GAAGAATCTC AGAGCCCCAT GAAAACAGAT CTGCTTCTGG TTAACCACAA ACCTAGTCCA
CAGATCATAC CATCAATGTC TGTGTCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA
TGCAGGAATC TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTGTTGGA TAAATGTCCA
CCTCCAAGAC CACCATCTTC ACCATACCCT CCCTTGCCAA AGGACAAGTT GAATCCACCT
ACACCTAGTA TTTACTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACATCAATTT
TGTACAAATC CGAACAACCC TGTTACAGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTA
GACCTGGGAC TTTTCTCTAC TAAAACTTTG GTGGAAGCTA ACAATGAACA TATGGTAGAA
GTGAGGACAC AGTTGTTGCA GCCAGCAGAT GAAAACTGGG ATCCCACTGG AACAAAGAAA
ATCTGGCATT GTGAAAGTAA TAGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG
GCCTCCTCAT TCCAGGAATC ATTGAGAGAA GAAAATGAAA AAAGAAGTCA TCATAAAGAC
CACTCAGATA GTGAATCTAC ATCGTCAGAT AATTCTGGGA GGAGGAGGAA AGGACCCTTT
AAAACCATAA AGTTTGGGAC CAATATTGAC CTATCTGATG ACAAAAAGTG GAAGTTGCAG
CTACATGAGC TGACTAAACT TCCTGCTTTT GTGCGTGTCG TATCAGCAGG AAATCTTCTA
AGCCATGTTG GTCATACCAT ATTGGGCATG AACACAGTTC AACTATACAT GAAAGTTCCA
GGGAGCAGAA CACCAGGTCA TCAGGAAAAT AACAACTTCT GTTCAGTTAA CATAAATATT
GGCCCAGGTG ACTGTGAATG GTTTGTTGTT CCTGAAGGTT ACTGGGGTGT TCTGAATGAC
TTCTGTGAAA AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT
CTTTATGAAG CAAATGTTCC AGTGTATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG
ATAAATGCAG GCACTGTTCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG
AATGTTGGTC CACTTACAGC CTGCCAGTAT AAATTGGCAG TGGAACGGTA CGAATGGAAC
AAATTGCAAA GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA
AATATCAAGG TCTCAGATCC AAAGCTTTTT GAAATGATTA AGATATTTTC ATTGTCTCCG
AATTTTAGAG CTGAAAAGTG CCTTAGAGAT CATCTAGTTC AACCTCTCCG TTCAAATGGA
GAACCTGAGC CACTAAGATT CACAGGAGAG TATTGTCTTC TAAGAACTCT GAAGCAATGT
CAGACATTGA GGGAAGCTCT CATTGCTGCA GGAAAAGAGA TTATATGGCA TGGGCGGACA
AAAGAAGAAC CAGCTCATTA CTGTAGCATT TGTGAAGTGG AGGTTTTTGA TCTGCTTTTT
GTCACTAATG AGAGTAATTC ACGAAAGACC TACATAGTAC ATTGCCAAGA TTGTGCACGA
AAAACAAGCG GAAACTTGGA AAACTTTGTG GTGCTAGAAC AGTACAAAAT GGAGGACCTG
ATGCAAGTCT ATGACCAATT TACATTAGCT CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61267
Clone ID Related Accession (Same CDS sequence) XM_011543976
Accession Version XM_011543976.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform X22
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG
ACACCACAGA AATTACAGCA TTTGGAACAG CTCCGCGCAA ATAGAAATAA TTTAAATCCA
GCACAGAAAC TGATGCTGGA ACAGCTGGAA AGTCAGTTTG TCTTAATGCA ACAACACCAA
ATGAGACCAA CAGGAGTTGC ACAGGTACGA TCTACTGGAA TTCCTAATGG GCCAACAGCT
GACTCATCAC TGCCTACAAA CTCAGTCTCT GGCCAGCAGC CACAGCTTGC TCTGACCAGA
GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT
GGACCCTTTT CTGCAGGCCA TGTTCCCTGT AGCACATCAA GAACGCTGGG AAGTACAGAC
ACTATTTTGA TAGGCAATAA TCATATAACA GGAAGTGGAA GTAATGGAAA CGTGCCTTAC
CTGCAGCGAA ACGCACTCAC TCTACCTCAT AACCGCACAA ACCTGACCAG CAGCGCAGAG
GAGCCGTGGA AAAACCAACT ATCTAACTCC ACTCAGGGGC TTCACAAAGG TCAGAGTTCA
CATTCGGCAG GTCCTAATGG TGAACGACCT CTCTCTTCCA CTGGGCCTTC CCAGCATCTC
CAGGCAGCTG GCTCTGGTAT TCAGAATCAG AACGGACATC CCACCCTGCC TAGCAATTCA
GTAACACAGG GGGCTGCTCT CAATCACCTC TCCTCTCACA CTGCTACCTC AGGTGGACAA
CAAGGCATTA CCTTAACCAA AGAGAGCAAG CCTTCAGGAA ACATATTGAC GGTGCCTGAA
ACAAGCAGGC ACACTGGAGA GACACCTAAC AGCACTGCCA GTGTCGAGGG ACTTCCTAAT
CATGTCCATC AGATGACGGC AGATGCTGTT TGCAGTCCTA GCCATGGAGA TTCTAAGTCA
CCAGGTTTAC TAAGTTCAGA CAATCCTCAG CTCTCTGCCT TGTTGATGGG AAAAGCCAAT
AACAATGTGG GTACTGGAAC CTGTGACAAA GTCAATAACA TCCACCCAGC TGTTCATACA
AAGACTGATA ACTCTGTTGC CTCTTCACCA TCTTCAGCCA TTTCAACAGC AACACCTTCT
CCAAAATCCA CTGAGCAGAC AACCACAAAC AGTGTTACCA GCCTTAACAG CCCTCACAGT
GGGCTACACA CAATTAATGG AGAAGGGATG GAAGAATCTC AGAGCCCCAT GAAAACAGAT
CTGCTTCTGG TTAACCACAA ACCTAGTCCA CAGATCATAC CATCAATGTC TGTGTCCATA
TACCCCAGCT CAGCAGAAGT TCTGAAGGCA TGCAGGAATC TAGGTAAAAA TGGCTTATCT
AACAGTAGCA TTTTGTTGGA TAAATGTCCA CCTCCAAGAC CACCATCTTC ACCATACCCT
CCCTTGCCAA AGGACAAGTT GAATCCACCT ACACCTAGTA TTTACTTGGA AAATAAACGT
GATGCTTTCT TTCCTCCATT ACATCAATTT TGTACAAATC CGAACAACCC TGTTACAGTA
ATACGTGGCC TTGCTGGAGC TCTTAAGTTA GACCTGGGAC TTTTCTCTAC TAAAACTTTG
GTGGAAGCTA ACAATGAACA TATGGTAGAA GTGAGGACAC AGTTGTTGCA GCCAGCAGAT
GAAAACTGGG ATCCCACTGG AACAAAGAAA ATCTGGCATT GTGAAAGTAA TAGATCTCAT
ACTACAATTG CTAAATATGC ACAGTACCAG GCCTCCTCAT TCCAGGAATC ATTGAGAGAA
GAAAATGAAA AAAGAAGTCA TCATAAAGAC CACTCAGATA GTGAATCTAC ATCGTCAGAT
AATTCTGGGA GGAGGAGGAA AGGACCCTTT AAAACCATAA AGTTTGGGAC CAATATTGAC
CTATCTGATG ACAAAAAGTG GAAGTTGCAG CTACATGAGC TGACTAAACT TCCTGCTTTT
GTGCGTGTCG TATCAGCAGG AAATCTTCTA AGCCATGTTG GTCATACCAT ATTGGGCATG
AACACAGTTC AACTATACAT GAAAGTTCCA GGGAGCAGAA CACCAGAAAT AATTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542278.1
CDS378..4034
Misc Feature(1)654..740(+)
Misc Feature(2)657..971(+)
Misc Feature(3)810..1631(+)
Misc Feature(4)819..911(+)
Misc Feature(5)822..923(+)
Misc Feature(6)924..1016(+)
Misc Feature(7)1047..1133(+)
Misc Feature(8)1050..1322(+)
Misc Feature(9)1146..1271(+)
Misc Feature(10)1248..1343(+)
Misc Feature(11)1284..1370(+)
Misc Feature(12)1287..1526(+)
Misc Feature(13)1287..1370(+)
Misc Feature(14)1383..1475(+)
Misc Feature(15)1386..1487(+)
Misc Feature(16)1488..1568(+)
Misc Feature(17)3885..>4022(+)
Translation

Target ORF information:

RefSeq Version XM_011543976
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant X23, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543976

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG
ACACCACAGA AATTACAGCA TTTGGAACAG CTCCGCGCAA ATAGAAATAA TTTAAATCCA
GCACAGAAAC TGATGCTGGA ACAGCTGGAA AGTCAGTTTG TCTTAATGCA ACAACACCAA
ATGAGACCAA CAGGAGTTGC ACAGGTACGA TCTACTGGAA TTCCTAATGG GCCAACAGCT
GACTCATCAC TGCCTACAAA CTCAGTCTCT GGCCAGCAGC CACAGCTTGC TCTGACCAGA
GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT
GGACCCTTTT CTGCAGGCCA TGTTCCCTGT AGCACATCAA GAACGCTGGG AAGTACAGAC
ACTATTTTGA TAGGCAATAA TCATATAACA GGAAGTGGAA GTAATGGAAA CGTGCCTTAC
CTGCAGCGAA ACGCACTCAC TCTACCTCAT AACCGCACAA ACCTGACCAG CAGCGCAGAG
GAGCCGTGGA AAAACCAACT ATCTAACTCC ACTCAGGGGC TTCACAAAGG TCAGAGTTCA
CATTCGGCAG GTCCTAATGG TGAACGACCT CTCTCTTCCA CTGGGCCTTC CCAGCATCTC
CAGGCAGCTG GCTCTGGTAT TCAGAATCAG AACGGACATC CCACCCTGCC TAGCAATTCA
GTAACACAGG GGGCTGCTCT CAATCACCTC TCCTCTCACA CTGCTACCTC AGGTGGACAA
CAAGGCATTA CCTTAACCAA AGAGAGCAAG CCTTCAGGAA ACATATTGAC GGTGCCTGAA
ACAAGCAGGC ACACTGGAGA GACACCTAAC AGCACTGCCA GTGTCGAGGG ACTTCCTAAT
CATGTCCATC AGATGACGGC AGATGCTGTT TGCAGTCCTA GCCATGGAGA TTCTAAGTCA
CCAGGTTTAC TAAGTTCAGA CAATCCTCAG CTCTCTGCCT TGTTGATGGG AAAAGCCAAT
AACAATGTGG GTACTGGAAC CTGTGACAAA GTCAATAACA TCCACCCAGC TGTTCATACA
AAGACTGATA ACTCTGTTGC CTCTTCACCA TCTTCAGCCA TTTCAACAGC AACACCTTCT
CCAAAATCCA CTGAGCAGAC AACCACAAAC AGTGTTACCA GCCTTAACAG CCCTCACAGT
GGGCTACACA CAATTAATGG AGAAGGGATG GAAGAATCTC AGAGCCCCAT GAAAACAGAT
CTGCTTCTGG TTAACCACAA ACCTAGTCCA CAGATCATAC CATCAATGTC TGTGTCCATA
TACCCCAGCT CAGCAGAAGT TCTGAAGGCA TGCAGGAATC TAGGTAAAAA TGGCTTATCT
AACAGTAGCA TTTTGTTGGA TAAATGTCCA CCTCCAAGAC CACCATCTTC ACCATACCCT
CCCTTGCCAA AGGACAAGTT GAATCCACCT ACACCTAGTA TTTACTTGGA AAATAAACGT
GATGCTTTCT TTCCTCCATT ACATCAATTT TGTACAAATC CGAACAACCC TGTTACAGTA
ATACGTGGCC TTGCTGGAGC TCTTAAGTTA GACCTGGGAC TTTTCTCTAC TAAAACTTTG
GTGGAAGCTA ACAATGAACA TATGGTAGAA GTGAGGACAC AGTTGTTGCA GCCAGCAGAT
GAAAACTGGG ATCCCACTGG AACAAAGAAA ATCTGGCATT GTGAAAGTAA TAGATCTCAT
ACTACAATTG CTAAATATGC ACAGTACCAG GCCTCCTCAT TCCAGGAATC ATTGAGAGAA
GAAAATGAAA AAAGAAGTCA TCATAAAGAC CACTCAGATA GTGAATCTAC ATCGTCAGAT
AATTCTGGGA GGAGGAGGAA AGGACCCTTT AAAACCATAA AGTTTGGGAC CAATATTGAC
CTATCTGATG ACAAAAAGTG GAAGTTGCAG CTACATGAGC TGACTAAACT TCCTGCTTTT
GTGCGTGTCG TATCAGCAGG AAATCTTCTA AGCCATGTTG GTCATACCAT ATTGGGCATG
AACACAGTTC AACTATACAT GAAAGTTCCA GGGAGCAGAA CACCAGAAAT AATTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24601
Clone ID Related Accession (Same CDS sequence) NM_001291415
Accession Version NM_001291415.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4362bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381812.1, BC143277.1, BC143278.1, AL831996.2, AL041377.1 and AI364446.1. This sequence is a reference standard in the RefSeqGene project. On Apr 12, 2014 this sequence version replaced gi:578838074. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143277.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT
TTGACACCAC AGAAATTACA GCATTTGGAA CAGCTCCGCG CAAATAGAAA TAATTTAAAT
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC
CAAATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT
TTTGAAATGA TTAAGTATTG TCTTCTAAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCT
CATTACTGTA GCATTTGTGA AGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT
AATTCACGAA AGACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGCGGAAAC
TTGGAAAACT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278344.1
CDS378..4739
Misc Feature(1)360..362(+)
Misc Feature(2)654..740(+)
Misc Feature(3)657..914(+)
Misc Feature(4)693..1574(+)
Misc Feature(5)762..854(+)
Misc Feature(6)765..866(+)
Misc Feature(7)867..959(+)
Misc Feature(8)990..1076(+)
Misc Feature(9)993..1265(+)
Misc Feature(10)1089..1214(+)
Misc Feature(11)1191..1286(+)
Misc Feature(12)1227..1313(+)
Misc Feature(13)1230..1469(+)
Misc Feature(14)1230..1313(+)
Misc Feature(15)1326..1418(+)
Misc Feature(16)1329..1430(+)
Misc Feature(17)1431..1511(+)
Misc Feature(18)3828..4022(+)
Misc Feature(19)3930..4256(+)
Exon (1)1..538
Gene:KDM6A
Gene Synonym:
Exon (2)539..602
Gene:KDM6A
Gene Synonym:
Exon (3)603..711
Gene:KDM6A
Gene Synonym:
Exon (4)712..761
Gene:KDM6A
Gene Synonym:
Exon (5)762..820
Gene:KDM6A
Gene Synonym:
Exon (6)821..941
Gene:KDM6A
Gene Synonym:
Exon (7)942..996
Gene:KDM6A
Gene Synonym:
Exon (8)997..1031
Gene:KDM6A
Gene Synonym:
Exon (9)1032..1125
Gene:KDM6A
Gene Synonym:
Exon (10)1126..1252
Gene:KDM6A
Gene Synonym:
Exon (11)1253..1351
Gene:KDM6A
Gene Synonym:
Exon (12)1352..1571
Gene:KDM6A
Gene Synonym:
Exon (13)1572..1706
Gene:KDM6A
Gene Synonym:
Exon (14)1707..1862
Gene:KDM6A
Gene Synonym:
Exon (15)1863..1958
Gene:KDM6A
Gene Synonym:
Exon (16)1959..2060
Gene:KDM6A
Gene Synonym:
Exon (17)2061..2456
Gene:KDM6A
Gene Synonym:
Exon (18)2457..3235
Gene:KDM6A
Gene Synonym:
Exon (19)3236..3365
Gene:KDM6A
Gene Synonym:
Exon (20)3366..3471
Gene:KDM6A
Gene Synonym:
Exon (21)3472..3677
Gene:KDM6A
Gene Synonym:
Exon (22)3678..3742
Gene:KDM6A
Gene Synonym:
Exon (23)3743..3817
Gene:KDM6A
Gene Synonym:
Exon (24)3818..3966
Gene:KDM6A
Gene Synonym:
Exon (25)3967..4081
Gene:KDM6A
Gene Synonym:
Exon (26)4082..4269
Gene:KDM6A
Gene Synonym:
Exon (27)4270..4411
Gene:KDM6A
Gene Synonym:
Exon (28)4412..4538
Gene:KDM6A
Gene Synonym:
Exon (29)4539..4709
Gene:KDM6A
Gene Synonym:
Exon (30)4710..5940
Gene:KDM6A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001291415
Organism Homo sapiens (human)
Definition Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001291415

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT
TTGACACCAC AGAAATTACA GCATTTGGAA CAGCTCCGCG CAAATAGAAA TAATTTAAAT
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC
CAAATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT
TTTGAAATGA TTAAGTATTG TCTTCTAAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCT
CATTACTGTA GCATTTGTGA AGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT
AATTCACGAA AGACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGCGGAAAC
TTGGAAAACT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24588
Clone ID Related Accession (Same CDS sequence) NM_001291416
Accession Version NM_001291416.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381812.1, BC143272.1, BC143278.1, AL831996.2, AL041377.1 and AI364446.1. On Apr 12, 2014 this sequence version replaced gi:578838076. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143272.1, EU026283.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA
TTACAGCATT TGGAACAGCT CCGCGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACCAACA
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC
TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA
GGCAATAATC ATATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTCGGCAGGT
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC
TCTGGTATTC AGAATCAGAA CGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ATATTGACGG TGCCTGAAAC AAGCAGGCAC
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG
ATGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAC
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCAACAGCAA CACCTTCTCC AAAATCCACT
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTACACACA
ATTAATGGAG AAGGGATGGA AGAATCTCAG AGCCCCATGA AAACAGATCT GCTTCTGGTT
AACCACAAAC CTAGTCCACA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT
TTGTTGGATA AATGTCCACC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG
GACAAGTTGA ATCCACCTAC ACCTAGTATT TACTTGGAAA ATAAACGTGA TGCTTTCTTT
CCTCCATTAC ATCAATTTTG TACAAATCCG AACAACCCTG TTACAGTAAT ACGTGGCCTT
GCTGGAGCTC TTAAGTTAGA CCTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA AAACTGGGAT
CCCACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT
AAATATGCAC AGTACCAGGC CTCCTCATTC CAGGAATCAT TGAGAGAAGA AAATGAAAAA
AGAAGTCATC ATAAAGACCA CTCAGATAGT GAATCTACAT CGTCAGATAA TTCTGGGAGG
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ATATTGACCT ATCTGATGAC
AAAAAGTGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA
TCAGCAGGAA ATCTTCTAAG CCATGTTGGT CATACCATAT TGGGCATGAA CACAGTTCAA
CTATACATGA AAGTTCCAGG GAGCAGAACA CCAGGTCATC AGGAAAATAA CAACTTCTGT
TCAGTTAACA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGTTAC
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG
TGGCCCAATC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG
TATTGTCTTC TAAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA
GGAAAAGAGA TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCTCATTA CTGTAGCATT
TGTGAAGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC ACGAAAGACC
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGCG GAAACTTGGA AAACTTTGTG
GTGCTAGAAC AGTACAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT
CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278345.1
CDS378..4604
Misc Feature(1)360..362(+)
Misc Feature(2)654..740(+)
Misc Feature(3)657..914(+)
Misc Feature(4)693..1580(+)
Misc Feature(5)762..854(+)
Misc Feature(6)765..866(+)
Misc Feature(7)867..959(+)
Misc Feature(8)990..1076(+)
Misc Feature(9)993..1265(+)
Misc Feature(10)1089..1214(+)
Misc Feature(11)1191..1286(+)
Misc Feature(12)1227..1313(+)
Misc Feature(13)1230..1469(+)
Misc Feature(14)1230..1313(+)
Misc Feature(15)1326..1418(+)
Misc Feature(16)1329..1430(+)
Misc Feature(17)1431..1511(+)
Misc Feature(18)3693..3887(+)
Misc Feature(19)3795..4121(+)
Exon (1)1..538
Gene:KDM6A
Gene Synonym:
Exon (2)539..602
Gene:KDM6A
Gene Synonym:
Exon (3)603..711
Gene:KDM6A
Gene Synonym:
Exon (4)712..761
Gene:KDM6A
Gene Synonym:
Exon (5)762..820
Gene:KDM6A
Gene Synonym:
Exon (6)821..941
Gene:KDM6A
Gene Synonym:
Exon (7)942..996
Gene:KDM6A
Gene Synonym:
Exon (8)997..1031
Gene:KDM6A
Gene Synonym:
Exon (9)1032..1125
Gene:KDM6A
Gene Synonym:
Exon (10)1126..1252
Gene:KDM6A
Gene Synonym:
Exon (11)1253..1351
Gene:KDM6A
Gene Synonym:
Exon (12)1352..1571
Gene:KDM6A
Gene Synonym:
Exon (13)1572..1727
Gene:KDM6A
Gene Synonym:
Exon (14)1728..1823
Gene:KDM6A
Gene Synonym:
Exon (15)1824..1925
Gene:KDM6A
Gene Synonym:
Exon (16)1926..2321
Gene:KDM6A
Gene Synonym:
Exon (17)2322..3100
Gene:KDM6A
Gene Synonym:
Exon (18)3101..3230
Gene:KDM6A
Gene Synonym:
Exon (19)3231..3336
Gene:KDM6A
Gene Synonym:
Exon (20)3337..3542
Gene:KDM6A
Gene Synonym:
Exon (21)3543..3607
Gene:KDM6A
Gene Synonym:
Exon (22)3608..3682
Gene:KDM6A
Gene Synonym:
Exon (23)3683..3831
Gene:KDM6A
Gene Synonym:
Exon (24)3832..3946
Gene:KDM6A
Gene Synonym:
Exon (25)3947..4134
Gene:KDM6A
Gene Synonym:
Exon (26)4135..4276
Gene:KDM6A
Gene Synonym:
Exon (27)4277..4403
Gene:KDM6A
Gene Synonym:
Exon (28)4404..4574
Gene:KDM6A
Gene Synonym:
Exon (29)4575..5805
Gene:KDM6A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001291416
Organism Homo sapiens (human)
Definition Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001291416

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA
TTACAGCATT TGGAACAGCT CCGCGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACCAACA
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC
TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA
GGCAATAATC ATATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTCGGCAGGT
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC
TCTGGTATTC AGAATCAGAA CGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ATATTGACGG TGCCTGAAAC AAGCAGGCAC
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG
ATGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAC
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCAACAGCAA CACCTTCTCC AAAATCCACT
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTACACACA
ATTAATGGAG AAGGGATGGA AGAATCTCAG AGCCCCATGA AAACAGATCT GCTTCTGGTT
AACCACAAAC CTAGTCCACA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT
TTGTTGGATA AATGTCCACC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG
GACAAGTTGA ATCCACCTAC ACCTAGTATT TACTTGGAAA ATAAACGTGA TGCTTTCTTT
CCTCCATTAC ATCAATTTTG TACAAATCCG AACAACCCTG TTACAGTAAT ACGTGGCCTT
GCTGGAGCTC TTAAGTTAGA CCTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA AAACTGGGAT
CCCACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT
AAATATGCAC AGTACCAGGC CTCCTCATTC CAGGAATCAT TGAGAGAAGA AAATGAAAAA
AGAAGTCATC ATAAAGACCA CTCAGATAGT GAATCTACAT CGTCAGATAA TTCTGGGAGG
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ATATTGACCT ATCTGATGAC
AAAAAGTGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA
TCAGCAGGAA ATCTTCTAAG CCATGTTGGT CATACCATAT TGGGCATGAA CACAGTTCAA
CTATACATGA AAGTTCCAGG GAGCAGAACA CCAGGTCATC AGGAAAATAA CAACTTCTGT
TCAGTTAACA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGTTAC
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG
TGGCCCAATC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG
TATTGTCTTC TAAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA
GGAAAAGAGA TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCTCATTA CTGTAGCATT
TGTGAAGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC ACGAAAGACC
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGCG GAAACTTGGA AAACTTTGTG
GTGCTAGAAC AGTACAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT
CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24583
Clone ID Related Accession (Same CDS sequence) NM_021140
Accession Version NM_021140.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381812.1, AB208795.1, AL041377.1 and AI364446.1. On Apr 12, 2014 this sequence version replaced gi:189011543. Transcript Variant: This variant (3) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF000993.1, AB208795.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT AAGAACTCTG
AAGCAATGTC AGACATTGAG GGAAGCTCTC ATTGCTGCAG GAAAAGAGAT TATATGGCAT
GGGCGGACAA AAGAAGAACC AGCTCATTAC TGTAGCATTT GTGAAGTGGA GGTTTTTGAT
CTGCTTTTTG TCACTAATGA GAGTAATTCA CGAAAGACCT ACATAGTACA TTGCCAAGAT
TGTGCACGAA AAACAAGCGG AAACTTGGAA AACTTTGTGG TGCTAGAACA GTACAAAATG
GAGGACCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC CTCCATTACC ATCCGCCTCA
TCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_066963.2
CDS378..4583
Misc Feature(1)360..362(+)
Misc Feature(2)378..3662(+)
Misc Feature(3)654..740(+)
Misc Feature(4)657..914(+)
Misc Feature(5)693..1574(+)
Misc Feature(6)762..854(+)
Misc Feature(7)765..866(+)
Misc Feature(8)867..959(+)
Misc Feature(9)990..1076(+)
Misc Feature(10)993..1265(+)
Misc Feature(11)1089..1214(+)
Misc Feature(12)1191..1286(+)
Misc Feature(13)1227..1313(+)
Misc Feature(14)1230..1469(+)
Misc Feature(15)1230..1313(+)
Misc Feature(16)1326..1418(+)
Misc Feature(17)1329..1430(+)
Misc Feature(18)1431..1511(+)
Misc Feature(19)2682..2684(+)
Misc Feature(20)2862..2864(+)
Misc Feature(21)3672..3866(+)
Misc Feature(22)3774..4100(+)
Exon (1)1..538
Gene:KDM6A
Gene Synonym:
Exon (2)539..602
Gene:KDM6A
Gene Synonym:
Exon (3)603..711
Gene:KDM6A
Gene Synonym:
Exon (4)712..761
Gene:KDM6A
Gene Synonym:
Exon (5)762..820
Gene:KDM6A
Gene Synonym:
Exon (6)821..941
Gene:KDM6A
Gene Synonym:
Exon (7)942..996
Gene:KDM6A
Gene Synonym:
Exon (8)997..1031
Gene:KDM6A
Gene Synonym:
Exon (9)1032..1125
Gene:KDM6A
Gene Synonym:
Exon (10)1126..1252
Gene:KDM6A
Gene Synonym:
Exon (11)1253..1351
Gene:KDM6A
Gene Synonym:
Exon (12)1352..1571
Gene:KDM6A
Gene Synonym:
Exon (13)1572..1706
Gene:KDM6A
Gene Synonym:
Exon (14)1707..1802
Gene:KDM6A
Gene Synonym:
Exon (15)1803..1904
Gene:KDM6A
Gene Synonym:
Exon (16)1905..2300
Gene:KDM6A
Gene Synonym:
Exon (17)2301..3079
Gene:KDM6A
Gene Synonym:
Exon (18)3080..3209
Gene:KDM6A
Gene Synonym:
Exon (19)3210..3315
Gene:KDM6A
Gene Synonym:
Exon (20)3316..3521
Gene:KDM6A
Gene Synonym:
Exon (21)3522..3586
Gene:KDM6A
Gene Synonym:
Exon (22)3587..3661
Gene:KDM6A
Gene Synonym:
Exon (23)3662..3810
Gene:KDM6A
Gene Synonym:
Exon (24)3811..3925
Gene:KDM6A
Gene Synonym:
Exon (25)3926..4113
Gene:KDM6A
Gene Synonym:
Exon (26)4114..4255
Gene:KDM6A
Gene Synonym:
Exon (27)4256..4382
Gene:KDM6A
Gene Synonym:
Exon (28)4383..4553
Gene:KDM6A
Gene Synonym:
Exon (29)4554..5784
Gene:KDM6A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_021140
Organism Homo sapiens (human)
Definition Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_021140

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT AAGAACTCTG
AAGCAATGTC AGACATTGAG GGAAGCTCTC ATTGCTGCAG GAAAAGAGAT TATATGGCAT
GGGCGGACAA AAGAAGAACC AGCTCATTAC TGTAGCATTT GTGAAGTGGA GGTTTTTGAT
CTGCTTTTTG TCACTAATGA GAGTAATTCA CGAAAGACCT ACATAGTACA TTGCCAAGAT
TGTGCACGAA AAACAAGCGG AAACTTGGAA AACTTTGTGG TGCTAGAACA GTACAAAATG
GAGGACCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC CTCCATTACC ATCCGCCTCA
TCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24587
Clone ID Related Accession (Same CDS sequence) NM_001291417
Accession Version NM_001291417.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4071bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381812.1, BC143278.1, AK303430.1, AL831996.2, AL041377.1 and AI364446.1. On Apr 12, 2014 this sequence version replaced gi:578838079. Transcript Variant: This variant (4) lacks two consecutive in-frame exons in the coding region, compared to variant 1. The resulting isoform (4) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143278.1, DQ062678.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT
TCATGGTGTT TGACACCACA GAAATTACAG CATTTGGAAC AGCTCCGCGC AAATAGAAAT
AATTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG
CAACAACACC AAATGAGACC AACAGGAGTT GCACAGGTAC GATCTACTGG AATTCCTAAT
GGGCCAACAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT
GCTCTGACCA GAGTGCCTAG CGTCTCTCAG CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG
CCTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG
GGAAGTACAG ACACTATTTT GATAGGCAAT AATCATATAA CAGGAAGTGG AAGTAATGGA
AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA
GGTCAGAGTT CACATTCGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAACGGACA TCCCACCCTG
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACATATTG
ACGGTGCCTG AAACAAGCAG GCACACTGGA GAGACACCTA ACAGCACTGC CAGTGTCGAG
GGACTTCCTA ATCATGTCCA TCAGATGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG
GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA
GCTGTTCATA CAAAGACTGA TAACTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCAACA
GCAACACCTT CTCCAAAATC CACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC
AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGGA TGGAAGAATC TCAGAGCCCC
ATGAAAACAG ATCTGCTTCT GGTTAACCAC AAACCTAGTC CACAGATCAT ACCATCAATG
TCTGTGTCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA TCTAGGTAAA
AATGGCTTAT CTAACAGTAG CATTTTGTTG GATAAATGTC CACCTCCAAG ACCACCATCT
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTACTTG
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA TCCGAACAAC
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TAGACCTGGG ACTTTTCTCT
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTG
CAGCCAGCAG ATGAAAACTG GGATCCCACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT
AATAGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC ATTCCAGGAA
TCATTGAGAG AAGAAAATGA AAAAAGAAGT CATCATAAAG ACCACTCAGA TAGTGAATCT
ACATCGTCAG ATAATTCTGG GAGGAGGAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG
ACCAATATTG ACCTATCTGA TGACAAAAAG TGGAAGTTGC AGCTACATGA GCTGACTAAA
CTTCCTGCTT TTGTGCGTGT CGTATCAGCA GGAAATCTTC TAAGCCATGT TGGTCATACC
ATATTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGGAGCAG AACACCAGGT
CATCAGGAAA ATAACAACTT CTGTTCAGTT AACATAAATA TTGGCCCAGG TGACTGTGAA
TGGTTTGTTG TTCCTGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT
TTGAATTTCC TAATGGGTTC TTGGTGGCCC AATCTTGAAG ATCTTTATGA AGCAAATGTT
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTT
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA
GCCTGCCAGT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTAAGAA CTCTGAAGCA ATGTCAGACA
TTGAGGGAAG CTCTCATTGC TGCAGGAAAA GAGATTATAT GGCATGGGCG GACAAAAGAA
GAACCAGCTC ATTACTGTAG CATTTGTGAA GTGGAGGTTT TTGATCTGCT TTTTGTCACT
AATGAGAGTA ATTCACGAAA GACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA
AGCGGAAACT TGGAAAACTT TGTGGTGCTA GAACAGTACA AAATGGAGGA CCTGATGCAA
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCG CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278346.1
CDS378..4448
Misc Feature(1)360..362(+)
Misc Feature(2)651..860(+)
Misc Feature(3)654..740(+)
Misc Feature(4)657..914(+)
Misc Feature(5)759..959(+)
Misc Feature(6)762..854(+)
Misc Feature(7)765..866(+)
Misc Feature(8)867..959(+)
Misc Feature(9)990..1223(+)
Misc Feature(10)990..1076(+)
Misc Feature(11)993..1265(+)
Misc Feature(12)1089..1214(+)
Misc Feature(13)1191..1286(+)
Misc Feature(14)1221..1421(+)
Misc Feature(15)1227..1313(+)
Misc Feature(16)1230..1469(+)
Misc Feature(17)1230..1313(+)
Misc Feature(18)1326..1418(+)
Misc Feature(19)1329..1430(+)
Misc Feature(20)1431..1511(+)
Misc Feature(21)3537..3731(+)
Misc Feature(22)3639..3965(+)
Exon (1)1..538
Gene:KDM6A
Gene Synonym:
Exon (2)539..602
Gene:KDM6A
Gene Synonym:
Exon (3)603..711
Gene:KDM6A
Gene Synonym:
Exon (4)712..761
Gene:KDM6A
Gene Synonym:
Exon (5)762..820
Gene:KDM6A
Gene Synonym:
Exon (6)821..941
Gene:KDM6A
Gene Synonym:
Exon (7)942..996
Gene:KDM6A
Gene Synonym:
Exon (8)997..1031
Gene:KDM6A
Gene Synonym:
Exon (9)1032..1125
Gene:KDM6A
Gene Synonym:
Exon (10)1126..1252
Gene:KDM6A
Gene Synonym:
Exon (11)1253..1351
Gene:KDM6A
Gene Synonym:
Exon (12)1352..1571
Gene:KDM6A
Gene Synonym:
Exon (13)1572..1667
Gene:KDM6A
Gene Synonym:
Exon (14)1668..1769
Gene:KDM6A
Gene Synonym:
Exon (15)1770..2165
Gene:KDM6A
Gene Synonym:
Exon (16)2166..2944
Gene:KDM6A
Gene Synonym:
Exon (17)2945..3074
Gene:KDM6A
Gene Synonym:
Exon (18)3075..3180
Gene:KDM6A
Gene Synonym:
Exon (19)3181..3386
Gene:KDM6A
Gene Synonym:
Exon (20)3387..3451
Gene:KDM6A
Gene Synonym:
Exon (21)3452..3526
Gene:KDM6A
Gene Synonym:
Exon (22)3527..3675
Gene:KDM6A
Gene Synonym:
Exon (23)3676..3790
Gene:KDM6A
Gene Synonym:
Exon (24)3791..3978
Gene:KDM6A
Gene Synonym:
Exon (25)3979..4120
Gene:KDM6A
Gene Synonym:
Exon (26)4121..4247
Gene:KDM6A
Gene Synonym:
Exon (27)4248..4418
Gene:KDM6A
Gene Synonym:
Exon (28)4419..5649
Gene:KDM6A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001291417
Organism Homo sapiens (human)
Definition Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001291417

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT
TCATGGTGTT TGACACCACA GAAATTACAG CATTTGGAAC AGCTCCGCGC AAATAGAAAT
AATTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG
CAACAACACC AAATGAGACC AACAGGAGTT GCACAGGTAC GATCTACTGG AATTCCTAAT
GGGCCAACAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT
GCTCTGACCA GAGTGCCTAG CGTCTCTCAG CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG
CCTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG
GGAAGTACAG ACACTATTTT GATAGGCAAT AATCATATAA CAGGAAGTGG AAGTAATGGA
AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA
GGTCAGAGTT CACATTCGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAACGGACA TCCCACCCTG
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACATATTG
ACGGTGCCTG AAACAAGCAG GCACACTGGA GAGACACCTA ACAGCACTGC CAGTGTCGAG
GGACTTCCTA ATCATGTCCA TCAGATGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG
GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA
GCTGTTCATA CAAAGACTGA TAACTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCAACA
GCAACACCTT CTCCAAAATC CACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC
AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGGA TGGAAGAATC TCAGAGCCCC
ATGAAAACAG ATCTGCTTCT GGTTAACCAC AAACCTAGTC CACAGATCAT ACCATCAATG
TCTGTGTCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA TCTAGGTAAA
AATGGCTTAT CTAACAGTAG CATTTTGTTG GATAAATGTC CACCTCCAAG ACCACCATCT
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTACTTG
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA TCCGAACAAC
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TAGACCTGGG ACTTTTCTCT
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTG
CAGCCAGCAG ATGAAAACTG GGATCCCACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT
AATAGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC ATTCCAGGAA
TCATTGAGAG AAGAAAATGA AAAAAGAAGT CATCATAAAG ACCACTCAGA TAGTGAATCT
ACATCGTCAG ATAATTCTGG GAGGAGGAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG
ACCAATATTG ACCTATCTGA TGACAAAAAG TGGAAGTTGC AGCTACATGA GCTGACTAAA
CTTCCTGCTT TTGTGCGTGT CGTATCAGCA GGAAATCTTC TAAGCCATGT TGGTCATACC
ATATTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGGAGCAG AACACCAGGT
CATCAGGAAA ATAACAACTT CTGTTCAGTT AACATAAATA TTGGCCCAGG TGACTGTGAA
TGGTTTGTTG TTCCTGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT
TTGAATTTCC TAATGGGTTC TTGGTGGCCC AATCTTGAAG ATCTTTATGA AGCAAATGTT
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTT
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA
GCCTGCCAGT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTAAGAA CTCTGAAGCA ATGTCAGACA
TTGAGGGAAG CTCTCATTGC TGCAGGAAAA GAGATTATAT GGCATGGGCG GACAAAAGAA
GAACCAGCTC ATTACTGTAG CATTTGTGAA GTGGAGGTTT TTGATCTGCT TTTTGTCACT
AATGAGAGTA ATTCACGAAA GACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA
AGCGGAAACT TGGAAAACTT TGTGGTGCTA GAACAGTACA AAATGGAGGA CCTGATGCAA
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCG CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24605
Clone ID Related Accession (Same CDS sequence) NM_001291418
Accession Version NM_001291418.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381812.1, BC143278.1, DQ062677.1, AB208795.1, AL041377.1 and AI364446.1. On Apr 12, 2014 this sequence version replaced gi:578838080. Transcript Variant: This variant (5) lacks two consecutive in-frame exons and a single in-frame exon in the coding region, compared to variant 1. The resulting isoform (5) lacks two internal segments, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ062677.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT
TCATGGTGTT TGACACCACA GAAATTACAG ATGAGACCAA CAGGAGTTGC ACAGGTACGA
TCTACTGGAA TTCCTAATGG GCCAACAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT
GGCCAGCAGC CACAGCTTGC TCTGACCAGA GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT
CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT GGACCCTTTT CTGCAGGCCA TGTTCCCTGT
AGCACATCAA GAACGCTGGG AAGTACAGAC ACTATTTTGA TAGGCAATAA TCATATAACA
GGAAGTGGAA GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT
AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC
ACTCAGGGGC TTCACAAAGG TCAGAGTTCA CATTCGGCAG GTCCTAATGG TGAACGACCT
CTCTCTTCCA CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG
AACGGACATC CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC
TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG
CCTTCAGGAA ACATATTGAC GGTGCCTGAA ACAAGCAGGC ACACTGGAGA GACACCTAAC
AGCACTGCCA GTGTCGAGGG ACTTCCTAAT CATGTCCATC AGATGACGGC AGATGCTGTT
TGCAGTCCTA GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG
CTCTCTGCCT TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA
GTCAATAACA TCCACCCAGC TGTTCATACA AAGACTGATA ACTCTGTTGC CTCTTCACCA
TCTTCAGCCA TTTCAACAGC AACACCTTCT CCAAAATCCA CTGAGCAGAC AACCACAAAC
AGTGTTACCA GCCTTAACAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGGATG
GAAGAATCTC AGAGCCCCAT GAAAACAGAT CTGCTTCTGG TTAACCACAA ACCTAGTCCA
CAGATCATAC CATCAATGTC TGTGTCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA
TGCAGGAATC TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTGTTGGA TAAATGTCCA
CCTCCAAGAC CACCATCTTC ACCATACCCT CCCTTGCCAA AGGACAAGTT GAATCCACCT
ACACCTAGTA TTTACTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACATCAATTT
TGTACAAATC CGAACAACCC TGTTACAGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTA
GACCTGGGAC TTTTCTCTAC TAAAACTTTG GTGGAAGCTA ACAATGAACA TATGGTAGAA
GTGAGGACAC AGTTGTTGCA GCCAGCAGAT GAAAACTGGG ATCCCACTGG AACAAAGAAA
ATCTGGCATT GTGAAAGTAA TAGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG
GCCTCCTCAT TCCAGGAATC ATTGAGAGAA GAAAATGAAA AAAGAAGTCA TCATAAAGAC
CACTCAGATA GTGAATCTAC ATCGTCAGAT AATTCTGGGA GGAGGAGGAA AGGACCCTTT
AAAACCATAA AGTTTGGGAC CAATATTGAC CTATCTGATG ACAAAAAGTG GAAGTTGCAG
CTACATGAGC TGACTAAACT TCCTGCTTTT GTGCGTGTCG TATCAGCAGG AAATCTTCTA
AGCCATGTTG GTCATACCAT ATTGGGCATG AACACAGTTC AACTATACAT GAAAGTTCCA
GGGAGCAGAA CACCAGGTCA TCAGGAAAAT AACAACTTCT GTTCAGTTAA CATAAATATT
GGCCCAGGTG ACTGTGAATG GTTTGTTGTT CCTGAAGGTT ACTGGGGTGT TCTGAATGAC
TTCTGTGAAA AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT
CTTTATGAAG CAAATGTTCC AGTGTATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG
ATAAATGCAG GCACTGTTCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG
AATGTTGGTC CACTTACAGC CTGCCAGTAT AAATTGGCAG TGGAACGGTA CGAATGGAAC
AAATTGCAAA GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA
AATATCAAGG TCTCAGATCC AAAGCTTTTT GAAATGATTA AGTATTGTCT TCTAAGAACT
CTGAAGCAAT GTCAGACATT GAGGGAAGCT CTCATTGCTG CAGGAAAAGA GATTATATGG
CATGGGCGGA CAAAAGAAGA ACCAGCTCAT TACTGTAGCA TTTGTGAAGT GGAGGTTTTT
GATCTGCTTT TTGTCACTAA TGAGAGTAAT TCACGAAAGA CCTACATAGT ACATTGCCAA
GATTGTGCAC GAAAAACAAG CGGAAACTTG GAAAACTTTG TGGTGCTAGA ACAGTACAAA
ATGGAGGACC TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCGCC
TCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278347.1
CDS378..4346
Misc Feature(1)360..362(+)
Misc Feature(2)651..860(+)
Misc Feature(3)654..740(+)
Misc Feature(4)657..914(+)
Misc Feature(5)759..959(+)
Misc Feature(6)762..854(+)
Misc Feature(7)765..866(+)
Misc Feature(8)867..959(+)
Misc Feature(9)990..1223(+)
Misc Feature(10)990..1076(+)
Misc Feature(11)993..1265(+)
Misc Feature(12)1089..1214(+)
Misc Feature(13)1191..1286(+)
Misc Feature(14)1221..1421(+)
Misc Feature(15)1227..1313(+)
Misc Feature(16)1230..1469(+)
Misc Feature(17)1230..1313(+)
Misc Feature(18)1326..1418(+)
Misc Feature(19)1329..1430(+)
Misc Feature(20)1431..1511(+)
Misc Feature(21)3435..3629(+)
Misc Feature(22)3537..3863(+)
Exon (1)1..538
Gene:KDM6A
Gene Synonym:
Exon (2)539..602
Gene:KDM6A
Gene Synonym:
Exon (3)603..711
Gene:KDM6A
Gene Synonym:
Exon (4)712..761
Gene:KDM6A
Gene Synonym:
Exon (5)762..820
Gene:KDM6A
Gene Synonym:
Exon (6)821..941
Gene:KDM6A
Gene Synonym:
Exon (7)942..996
Gene:KDM6A
Gene Synonym:
Exon (8)997..1031
Gene:KDM6A
Gene Synonym:
Exon (9)1032..1125
Gene:KDM6A
Gene Synonym:
Exon (10)1126..1252
Gene:KDM6A
Gene Synonym:
Exon (11)1253..1351
Gene:KDM6A
Gene Synonym:
Exon (12)1352..1571
Gene:KDM6A
Gene Synonym:
Exon (13)1572..1667
Gene:KDM6A
Gene Synonym:
Exon (14)1668..2063
Gene:KDM6A
Gene Synonym:
Exon (15)2064..2842
Gene:KDM6A
Gene Synonym:
Exon (16)2843..2972
Gene:KDM6A
Gene Synonym:
Exon (17)2973..3078
Gene:KDM6A
Gene Synonym:
Exon (18)3079..3284
Gene:KDM6A
Gene Synonym:
Exon (19)3285..3349
Gene:KDM6A
Gene Synonym:
Exon (20)3350..3424
Gene:KDM6A
Gene Synonym:
Exon (21)3425..3573
Gene:KDM6A
Gene Synonym:
Exon (22)3574..3688
Gene:KDM6A
Gene Synonym:
Exon (23)3689..3876
Gene:KDM6A
Gene Synonym:
Exon (24)3877..4018
Gene:KDM6A
Gene Synonym:
Exon (25)4019..4145
Gene:KDM6A
Gene Synonym:
Exon (26)4146..4316
Gene:KDM6A
Gene Synonym:
Exon (27)4317..5547
Gene:KDM6A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001291418
Organism Homo sapiens (human)
Definition Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001291418

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT
TCATGGTGTT TGACACCACA GAAATTACAG ATGAGACCAA CAGGAGTTGC ACAGGTACGA
TCTACTGGAA TTCCTAATGG GCCAACAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT
GGCCAGCAGC CACAGCTTGC TCTGACCAGA GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT
CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT GGACCCTTTT CTGCAGGCCA TGTTCCCTGT
AGCACATCAA GAACGCTGGG AAGTACAGAC ACTATTTTGA TAGGCAATAA TCATATAACA
GGAAGTGGAA GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT
AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC
ACTCAGGGGC TTCACAAAGG TCAGAGTTCA CATTCGGCAG GTCCTAATGG TGAACGACCT
CTCTCTTCCA CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG
AACGGACATC CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC
TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG
CCTTCAGGAA ACATATTGAC GGTGCCTGAA ACAAGCAGGC ACACTGGAGA GACACCTAAC
AGCACTGCCA GTGTCGAGGG ACTTCCTAAT CATGTCCATC AGATGACGGC AGATGCTGTT
TGCAGTCCTA GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG
CTCTCTGCCT TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA
GTCAATAACA TCCACCCAGC TGTTCATACA AAGACTGATA ACTCTGTTGC CTCTTCACCA
TCTTCAGCCA TTTCAACAGC AACACCTTCT CCAAAATCCA CTGAGCAGAC AACCACAAAC
AGTGTTACCA GCCTTAACAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGGATG
GAAGAATCTC AGAGCCCCAT GAAAACAGAT CTGCTTCTGG TTAACCACAA ACCTAGTCCA
CAGATCATAC CATCAATGTC TGTGTCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA
TGCAGGAATC TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTGTTGGA TAAATGTCCA
CCTCCAAGAC CACCATCTTC ACCATACCCT CCCTTGCCAA AGGACAAGTT GAATCCACCT
ACACCTAGTA TTTACTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACATCAATTT
TGTACAAATC CGAACAACCC TGTTACAGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTA
GACCTGGGAC TTTTCTCTAC TAAAACTTTG GTGGAAGCTA ACAATGAACA TATGGTAGAA
GTGAGGACAC AGTTGTTGCA GCCAGCAGAT GAAAACTGGG ATCCCACTGG AACAAAGAAA
ATCTGGCATT GTGAAAGTAA TAGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG
GCCTCCTCAT TCCAGGAATC ATTGAGAGAA GAAAATGAAA AAAGAAGTCA TCATAAAGAC
CACTCAGATA GTGAATCTAC ATCGTCAGAT AATTCTGGGA GGAGGAGGAA AGGACCCTTT
AAAACCATAA AGTTTGGGAC CAATATTGAC CTATCTGATG ACAAAAAGTG GAAGTTGCAG
CTACATGAGC TGACTAAACT TCCTGCTTTT GTGCGTGTCG TATCAGCAGG AAATCTTCTA
AGCCATGTTG GTCATACCAT ATTGGGCATG AACACAGTTC AACTATACAT GAAAGTTCCA
GGGAGCAGAA CACCAGGTCA TCAGGAAAAT AACAACTTCT GTTCAGTTAA CATAAATATT
GGCCCAGGTG ACTGTGAATG GTTTGTTGTT CCTGAAGGTT ACTGGGGTGT TCTGAATGAC
TTCTGTGAAA AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT
CTTTATGAAG CAAATGTTCC AGTGTATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG
ATAAATGCAG GCACTGTTCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG
AATGTTGGTC CACTTACAGC CTGCCAGTAT AAATTGGCAG TGGAACGGTA CGAATGGAAC
AAATTGCAAA GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA
AATATCAAGG TCTCAGATCC AAAGCTTTTT GAAATGATTA AGTATTGTCT TCTAAGAACT
CTGAAGCAAT GTCAGACATT GAGGGAAGCT CTCATTGCTG CAGGAAAAGA GATTATATGG
CATGGGCGGA CAAAAGAAGA ACCAGCTCAT TACTGTAGCA TTTGTGAAGT GGAGGTTTTT
GATCTGCTTT TTGTCACTAA TGAGAGTAAT TCACGAAAGA CCTACATAGT ACATTGCCAA
GATTGTGCAC GAAAAACAAG CGGAAACTTG GAAAACTTTG TGGTGCTAGA ACAGTACAAA
ATGGAGGACC TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCGCC
TCATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24600
Clone ID Related Accession (Same CDS sequence) NM_001291421
Accession Version NM_001291421.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product lysine-specific demethylase 6A isoform 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381812.1, BC143275.1, BC143278.1, AL831996.2, AL041377.1 and AI364446.1. Transcript Variant: This variant (6) lacks an internal exon in the 5' region, which results in translation initiation from a downstream start codon, and also lacks two consecutive in-frame exons in the coding region, compared to variant 1. The resulting isoform (6) has a shorter N-terminus and lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143275.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGCATCACA CTGTAGATCT CCTGGGAGAT AAAGCCACCA AGGAAAGCTA TGCTATTCAG 
TATCTCCAAA AGTCCTTGGA AGCAGATCCT AATTCTGGCC AGTCCTGGTA TTTCCTCGGA
AGGTGCTATT CAAGTATTGG GAAAGTTCAG GATGCCTTTA TATCTTACAG GCAGTCTATT
GATAAATCAG AAGCAAGTGC AGATACATGG TGTTCAATAG GTGTGCTATA TCAGCAGCAA
AATCAGCCCA TGGATGCTTT ACAGGCCTAT ATTTGTGCTG TACAATTGGA CCATGGCCAT
GCTGCAGCCT GGATGGACCT AGGCACTCTC TATGAATCCT GCAACCAGCC TCAGGATGCC
ATTAAATGCT ACTTAAATGC AACTAGAAGC AAAAGTTGTA GTAATACCTC TGCACTTGCA
GCACGAATTA AGTATTTACA GAATACTTCT GACAATTGGA GTGGTGGACA TGCTGTGTCA
CATCCTCCAG TACAGCAACA AGCTCATTCA TGGTGTTTGA CACCACAGAA ATTACAGCAT
TTGGAACAGC TCCGCGCAAA TAGAAATAAT TTAAATCCAG CACAGAAACT GATGCTGGAA
CAGCTGGAAA GTCAGTTTGT CTTAATGCAA CAACACCAAA TGAGACCAAC AGGAGTTGCA
CAGGTACGAT CTACTGGAAT TCCTAATGGG CCAACAGCTG ACTCATCACT GCCTACAAAC
TCAGTCTCTG GCCAGCAGCC ACAGCTTGCT CTGACCAGAG TGCCTAGCGT CTCTCAGCCT
GGAGTCCGTC CTGCCTGCCC TGGGCAGCCT TTGGCCAATG GACCCTTTTC TGCAGGCCAT
GTTCCCTGTA GCACATCAAG AACGCTGGGA AGTACAGACA CTATTTTGAT AGGCAATAAT
CATATAACAG GAAGTGGAAG TAATGGAAAC GTGCCTTACC TGCAGCGAAA CGCACTCACT
CTACCTCATA ACCGCACAAA CCTGACCAGC AGCGCAGAGG AGCCGTGGAA AAACCAACTA
TCTAACTCCA CTCAGGGGCT TCACAAAGGT CAGAGTTCAC ATTCGGCAGG TCCTAATGGT
GAACGACCTC TCTCTTCCAC TGGGCCTTCC CAGCATCTCC AGGCAGCTGG CTCTGGTATT
CAGAATCAGA ACGGACATCC CACCCTGCCT AGCAATTCAG TAACACAGGG GGCTGCTCTC
AATCACCTCT CCTCTCACAC TGCTACCTCA GGTGGACAAC AAGGCATTAC CTTAACCAAA
GAGAGCAAGC CTTCAGGAAA CATATTGACG GTGCCTGAAA CAAGCAGGCA CACTGGAGAG
ACACCTAACA GCACTGCCAG TGTCGAGGGA CTTCCTAATC ATGTCCATCA GATGACGGCA
GATGCTGTTT GCAGTCCTAG CCATGGAGAT TCTAAGTCAC CAGGTTTACT AAGTTCAGAC
AATCCTCAGC TCTCTGCCTT GTTGATGGGA AAAGCCAATA ACAATGTGGG TACTGGAACC
TGTGACAAAG TCAATAACAT CCACCCAGCT GTTCATACAA AGACTGATAA CTCTGTTGCC
TCTTCACCAT CTTCAGCCAT TTCAACAGCA ACACCTTCTC CAAAATCCAC TGAGCAGACA
ACCACAAACA GTGTTACCAG CCTTAACAGC CCTCACAGTG GGCTACACAC AATTAATGGA
GAAGGGATGG AAGAATCTCA GAGCCCCATG AAAACAGATC TGCTTCTGGT TAACCACAAA
CCTAGTCCAC AGATCATACC ATCAATGTCT GTGTCCATAT ACCCCAGCTC AGCAGAAGTT
CTGAAGGCAT GCAGGAATCT AGGTAAAAAT GGCTTATCTA ACAGTAGCAT TTTGTTGGAT
AAATGTCCAC CTCCAAGACC ACCATCTTCA CCATACCCTC CCTTGCCAAA GGACAAGTTG
AATCCACCTA CACCTAGTAT TTACTTGGAA AATAAACGTG ATGCTTTCTT TCCTCCATTA
CATCAATTTT GTACAAATCC GAACAACCCT GTTACAGTAA TACGTGGCCT TGCTGGAGCT
CTTAAGTTAG ACCTGGGACT TTTCTCTACT AAAACTTTGG TGGAAGCTAA CAATGAACAT
ATGGTAGAAG TGAGGACACA GTTGTTGCAG CCAGCAGATG AAAACTGGGA TCCCACTGGA
ACAAAGAAAA TCTGGCATTG TGAAAGTAAT AGATCTCATA CTACAATTGC TAAATATGCA
CAGTACCAGG CCTCCTCATT CCAGGAATCA TTGAGAGAAG AAAATGAAAA AAGAAGTCAT
CATAAAGACC ACTCAGATAG TGAATCTACA TCGTCAGATA ATTCTGGGAG GAGGAGGAAA
GGACCCTTTA AAACCATAAA GTTTGGGACC AATATTGACC TATCTGATGA CAAAAAGTGG
AAGTTGCAGC TACATGAGCT GACTAAACTT CCTGCTTTTG TGCGTGTCGT ATCAGCAGGA
AATCTTCTAA GCCATGTTGG TCATACCATA TTGGGCATGA ACACAGTTCA ACTATACATG
AAAGTTCCAG GGAGCAGAAC ACCAGGTCAT CAGGAAAATA ACAACTTCTG TTCAGTTAAC
ATAAATATTG GCCCAGGTGA CTGTGAATGG TTTGTTGTTC CTGAAGGTTA CTGGGGTGTT
CTGAATGACT TCTGTGAAAA AAATAATTTG AATTTCCTAA TGGGTTCTTG GTGGCCCAAT
CTTGAAGATC TTTATGAAGC AAATGTTCCA GTGTATAGGT TTATTCAGCG ACCTGGAGAT
TTGGTCTGGA TAAATGCAGG CACTGTTCAT TGGGTTCAGG CTATTGGCTG GTGCAACAAC
ATTGCTTGGA ATGTTGGTCC ACTTACAGCC TGCCAGTATA AATTGGCAGT GGAACGGTAC
GAATGGAACA AATTGCAAAG TGTGAAGTCA ATAGTACCCA TGGTTCATCT TTCCTGGAAT
ATGGCACGAA ATATCAAGGT CTCAGATCCA AAGCTTTTTG AAATGATTAA GTATTGTCTT
CTAAGAACTC TGAAGCAATG TCAGACATTG AGGGAAGCTC TCATTGCTGC AGGAAAAGAG
ATTATATGGC ATGGGCGGAC AAAAGAAGAA CCAGCTCATT ACTGTAGCAT TTGTGAAGTG
GAGGTTTTTG ATCTGCTTTT TGTCACTAAT GAGAGTAATT CACGAAAGAC CTACATAGTA
CATTGCCAAG ATTGTGCACG AAAAACAAGC GGAAACTTGG AAAACTTTGT GGTGCTAGAA
CAGTACAAAA TGGAGGACCT GATGCAAGTC TATGACCAAT TTACATTAGC TCCTCCATTA
CCATCCGCCT CATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278350.1
CDS1010..4327
Misc Feature(1)806..808(+)
Misc Feature(2)1070..1165(+)
Misc Feature(3)1100..1300(+)
Misc Feature(4)1109..1348(+)
Misc Feature(5)1109..1192(+)
Misc Feature(6)1205..1297(+)
Misc Feature(7)1208..1309(+)
Misc Feature(8)1310..1390(+)
Misc Feature(9)3416..3610(+)
Misc Feature(10)3518..3844(+)
Exon (1)1..538
Gene:KDM6A
Gene Synonym:
Exon (2)539..602
Gene:KDM6A
Gene Synonym:
Exon (3)603..711
Gene:KDM6A
Gene Synonym:
Exon (4)712..761
Gene:KDM6A
Gene Synonym:
Exon (5)762..820
Gene:KDM6A
Gene Synonym:
Exon (6)821..875
Gene:KDM6A
Gene Synonym:
Exon (7)876..910
Gene:KDM6A
Gene Synonym:
Exon (8)911..1004
Gene:KDM6A
Gene Synonym:
Exon (9)1005..1131
Gene:KDM6A
Gene Synonym:
Exon (10)1132..1230
Gene:KDM6A
Gene Synonym:
Exon (11)1231..1450
Gene:KDM6A
Gene Synonym:
Exon (12)1451..1546
Gene:KDM6A
Gene Synonym:
Exon (13)1547..1648
Gene:KDM6A
Gene Synonym:
Exon (14)1649..2044
Gene:KDM6A
Gene Synonym:
Exon (15)2045..2823
Gene:KDM6A
Gene Synonym:
Exon (16)2824..2953
Gene:KDM6A
Gene Synonym:
Exon (17)2954..3059
Gene:KDM6A
Gene Synonym:
Exon (18)3060..3265
Gene:KDM6A
Gene Synonym:
Exon (19)3266..3330
Gene:KDM6A
Gene Synonym:
Exon (20)3331..3405
Gene:KDM6A
Gene Synonym:
Exon (21)3406..3554
Gene:KDM6A
Gene Synonym:
Exon (22)3555..3669
Gene:KDM6A
Gene Synonym:
Exon (23)3670..3857
Gene:KDM6A
Gene Synonym:
Exon (24)3858..3999
Gene:KDM6A
Gene Synonym:
Exon (25)4000..4126
Gene:KDM6A
Gene Synonym:
Exon (26)4127..4297
Gene:KDM6A
Gene Synonym:
Exon (27)4298..5528
Gene:KDM6A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001291421
Organism Homo sapiens (human)
Definition Homo sapiens lysine (K)-specific demethylase 6A (KDM6A), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001291421

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGCATCACA CTGTAGATCT CCTGGGAGAT AAAGCCACCA AGGAAAGCTA TGCTATTCAG 
TATCTCCAAA AGTCCTTGGA AGCAGATCCT AATTCTGGCC AGTCCTGGTA TTTCCTCGGA
AGGTGCTATT CAAGTATTGG GAAAGTTCAG GATGCCTTTA TATCTTACAG GCAGTCTATT
GATAAATCAG AAGCAAGTGC AGATACATGG TGTTCAATAG GTGTGCTATA TCAGCAGCAA
AATCAGCCCA TGGATGCTTT ACAGGCCTAT ATTTGTGCTG TACAATTGGA CCATGGCCAT
GCTGCAGCCT GGATGGACCT AGGCACTCTC TATGAATCCT GCAACCAGCC TCAGGATGCC
ATTAAATGCT ACTTAAATGC AACTAGAAGC AAAAGTTGTA GTAATACCTC TGCACTTGCA
GCACGAATTA AGTATTTACA GAATACTTCT GACAATTGGA GTGGTGGACA TGCTGTGTCA
CATCCTCCAG TACAGCAACA AGCTCATTCA TGGTGTTTGA CACCACAGAA ATTACAGCAT
TTGGAACAGC TCCGCGCAAA TAGAAATAAT TTAAATCCAG CACAGAAACT GATGCTGGAA
CAGCTGGAAA GTCAGTTTGT CTTAATGCAA CAACACCAAA TGAGACCAAC AGGAGTTGCA
CAGGTACGAT CTACTGGAAT TCCTAATGGG CCAACAGCTG ACTCATCACT GCCTACAAAC
TCAGTCTCTG GCCAGCAGCC ACAGCTTGCT CTGACCAGAG TGCCTAGCGT CTCTCAGCCT
GGAGTCCGTC CTGCCTGCCC TGGGCAGCCT TTGGCCAATG GACCCTTTTC TGCAGGCCAT
GTTCCCTGTA GCACATCAAG AACGCTGGGA AGTACAGACA CTATTTTGAT AGGCAATAAT
CATATAACAG GAAGTGGAAG TAATGGAAAC GTGCCTTACC TGCAGCGAAA CGCACTCACT
CTACCTCATA ACCGCACAAA CCTGACCAGC AGCGCAGAGG AGCCGTGGAA AAACCAACTA
TCTAACTCCA CTCAGGGGCT TCACAAAGGT CAGAGTTCAC ATTCGGCAGG TCCTAATGGT
GAACGACCTC TCTCTTCCAC TGGGCCTTCC CAGCATCTCC AGGCAGCTGG CTCTGGTATT
CAGAATCAGA ACGGACATCC CACCCTGCCT AGCAATTCAG TAACACAGGG GGCTGCTCTC
AATCACCTCT CCTCTCACAC TGCTACCTCA GGTGGACAAC AAGGCATTAC CTTAACCAAA
GAGAGCAAGC CTTCAGGAAA CATATTGACG GTGCCTGAAA CAAGCAGGCA CACTGGAGAG
ACACCTAACA GCACTGCCAG TGTCGAGGGA CTTCCTAATC ATGTCCATCA GATGACGGCA
GATGCTGTTT GCAGTCCTAG CCATGGAGAT TCTAAGTCAC CAGGTTTACT AAGTTCAGAC
AATCCTCAGC TCTCTGCCTT GTTGATGGGA AAAGCCAATA ACAATGTGGG TACTGGAACC
TGTGACAAAG TCAATAACAT CCACCCAGCT GTTCATACAA AGACTGATAA CTCTGTTGCC
TCTTCACCAT CTTCAGCCAT TTCAACAGCA ACACCTTCTC CAAAATCCAC TGAGCAGACA
ACCACAAACA GTGTTACCAG CCTTAACAGC CCTCACAGTG GGCTACACAC AATTAATGGA
GAAGGGATGG AAGAATCTCA GAGCCCCATG AAAACAGATC TGCTTCTGGT TAACCACAAA
CCTAGTCCAC AGATCATACC ATCAATGTCT GTGTCCATAT ACCCCAGCTC AGCAGAAGTT
CTGAAGGCAT GCAGGAATCT AGGTAAAAAT GGCTTATCTA ACAGTAGCAT TTTGTTGGAT
AAATGTCCAC CTCCAAGACC ACCATCTTCA CCATACCCTC CCTTGCCAAA GGACAAGTTG
AATCCACCTA CACCTAGTAT TTACTTGGAA AATAAACGTG ATGCTTTCTT TCCTCCATTA
CATCAATTTT GTACAAATCC GAACAACCCT GTTACAGTAA TACGTGGCCT TGCTGGAGCT
CTTAAGTTAG ACCTGGGACT TTTCTCTACT AAAACTTTGG TGGAAGCTAA CAATGAACAT
ATGGTAGAAG TGAGGACACA GTTGTTGCAG CCAGCAGATG AAAACTGGGA TCCCACTGGA
ACAAAGAAAA TCTGGCATTG TGAAAGTAAT AGATCTCATA CTACAATTGC TAAATATGCA
CAGTACCAGG CCTCCTCATT CCAGGAATCA TTGAGAGAAG AAAATGAAAA AAGAAGTCAT
CATAAAGACC ACTCAGATAG TGAATCTACA TCGTCAGATA ATTCTGGGAG GAGGAGGAAA
GGACCCTTTA AAACCATAAA GTTTGGGACC AATATTGACC TATCTGATGA CAAAAAGTGG
AAGTTGCAGC TACATGAGCT GACTAAACTT CCTGCTTTTG TGCGTGTCGT ATCAGCAGGA
AATCTTCTAA GCCATGTTGG TCATACCATA TTGGGCATGA ACACAGTTCA ACTATACATG
AAAGTTCCAG GGAGCAGAAC ACCAGGTCAT CAGGAAAATA ACAACTTCTG TTCAGTTAAC
ATAAATATTG GCCCAGGTGA CTGTGAATGG TTTGTTGTTC CTGAAGGTTA CTGGGGTGTT
CTGAATGACT TCTGTGAAAA AAATAATTTG AATTTCCTAA TGGGTTCTTG GTGGCCCAAT
CTTGAAGATC TTTATGAAGC AAATGTTCCA GTGTATAGGT TTATTCAGCG ACCTGGAGAT
TTGGTCTGGA TAAATGCAGG CACTGTTCAT TGGGTTCAGG CTATTGGCTG GTGCAACAAC
ATTGCTTGGA ATGTTGGTCC ACTTACAGCC TGCCAGTATA AATTGGCAGT GGAACGGTAC
GAATGGAACA AATTGCAAAG TGTGAAGTCA ATAGTACCCA TGGTTCATCT TTCCTGGAAT
ATGGCACGAA ATATCAAGGT CTCAGATCCA AAGCTTTTTG AAATGATTAA GTATTGTCTT
CTAAGAACTC TGAAGCAATG TCAGACATTG AGGGAAGCTC TCATTGCTGC AGGAAAAGAG
ATTATATGGC ATGGGCGGAC AAAAGAAGAA CCAGCTCATT ACTGTAGCAT TTGTGAAGTG
GAGGTTTTTG ATCTGCTTTT TGTCACTAAT GAGAGTAATT CACGAAAGAC CTACATAGTA
CATTGCCAAG ATTGTGCACG AAAAACAAGC GGAAACTTGG AAAACTTTGT GGTGCTAGAA
CAGTACAAAA TGGAGGACCT GATGCAAGTC TATGACCAAT TTACATTAGC TCCTCCATTA
CCATCCGCCT CATCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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