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VCL cDNA ORF clone, Homo sapiens (human)

Gene Symbol VCL
Entrez Gene ID 7414
Full Name vinculin
Synonyms CMD1W, CMH15, HEL114, MV, MVCL
General protein information
Preferred Names
vinculin
Names
vinculin
metavinculin
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q22.2

Summary Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

193065

Disorder Html: Cardiomyopathy, dilated, 1W, 611407 (3); Cardiomyopathy, familial

mRNA and Protein(s)

mRNA Protein Name
XM_005270142 XP_005270199 vinculin isoform X1
XM_005270143 XP_005270200 vinculin isoform X2
NM_014000 NP_054706 vinculin isoform meta-VCL
NM_003373 NP_003364 vinculin isoform VCL



Homo sapiens (human) VCL NP_054706.1
Pan troglodytes (chimpanzee) VCL XP_507854.2
Macaca mulatta (Rhesus monkey) VCL XP_001118118.1
Canis lupus familiaris (dog) VCL XP_536395.3
Bos taurus (cattle) VCL NP_001178299.1
Mus musculus (house mouse) Vcl NP_033528.3
Rattus norvegicus (Norway rat) Vcl NP_001100718.1
Gallus gallus (chicken) VCL NP_990772.1
Danio rerio (zebrafish) vcla NP_001122153.1
Xenopus (Silurana) tropicalis (western clawed frog) vcl NP_001090722.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu44532 XM_005270142 PREDICTED: Homo sapiens vinculin (VCL), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu44533 XM_005270143 PREDICTED: Homo sapiens vinculin (VCL), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu25616
NM_014000 Homo sapiens vinculin (VCL), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu25331
NM_003373 Homo sapiens vinculin (VCL), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu44532
Clone ID Related Accession (Same CDS sequence) XM_005270142
Accession Version XM_005270142.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product vinculin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG
GCCAAAGGTT GGCTCCGTGA CCCTAGTGCC TCCCCAGGGG ATGCTGGTGA GCAGGCCATC
AGACAGATCT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG
GAGATTCTGG GAACTTGCAA AATGCTAGGG CAGATGACTG ATCAAGTGGC TGACCTCCGT
GCCAGAGGAC AAGGATCCTC ACCGGTGGCC ATGCAGAAAG CTCAGCAGGT ATCTCAGGGT
CTGGATGTGC TCACAGCAAA AGTGGAAAAT GCAGCTCGCA AGCTGGAAGC CATGACCAAC
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ACTGGCTTGC AGATCCAAAT
GGTGGACCGG AAGGAGAAGA GCAGATTCGA GGTGCTTTGG CTGAAGCTCG GAAAATAGCA
GAATTATGTG ATGATCCTAA AGAAAGAGAT GACATTCTAC GTTCCCTTGG GGAAATATCT
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGGA AAGGAGATTC TCCAGAGGCT
CGAGCCTTGG CCAAACAGGT GGCCACGGCC CTGCAGAACC TGCAGACCAA AACCAACCGG
GCTGTGGCCA ACAGCAGACC GGCCAAAGCA GCTGTACACC TTGAGGGCAA GATTGAGCAA
GCACAGCGGT GGATTGATAA TCCCACAGTG GATGACCGTG GAGTCGGTCA GGCTGCCATC
CGGGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTTA TGATGGGGCC TTATCGGCAA
GATCTTCTCG CCAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCA CGAGCACTTG CATCTCAGCT CCAAGACTCC
TTAAAGGATC TAAAAGCTCG GATGCAGGAG GCCATGACTC AGGAAGTGTC AGATGTTTTC
AGCGATACCA CAACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACGGCGCC TCCTGATGCG
CCTAACAGGG AAGAGGTATT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAAG
CTTGGTGCTA CGGCCGAGAA GGCGGCTGCG GTTGGTACTG CTAATAAATC AACAGTGGAA
GGCATTCAGG CCTCAGTGAA GACGGCCCGA GAACTCACAC CCCAGGTGGT CTCGGCTGCT
CGTATCTTAC TTAGGAACCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGATACC
AAATCTCTGT TGGATGCTTC AGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGCAAGGTA
GCTATGGCCA ACATTCAGCC TCAGATGCTG GTTGCTGGGG CAACCAGTAT TGCTCGTCGG
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ATTCCGAGGA TCCCAAGTTC
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC GATGGTGATG
GATGCAAAAG CTGTGGCTGG AAACATTTCC GACCCTGGAC TGCAAAAGAG CTTCCTGGAC
TCAGGATATC GGATCCTGGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCTCAGGAG
CCTGACTTCC CGCCGCCTCC ACCAGACCTT GAACAACTCC GACTAACAGA TGAGCTTGCT
CCTCCCAAAC CACCTCTGCC TGAAGGTGAG GTCCCTCCAC CTAGGCCTCC ACCACCAGAG
GAAAAGGATG AAGAGTTCCC TGAGCAGAAG GCCGGGGAGG TGATTAACCA GCCAATGATG
ATGGCTGCCA GACAGCTCCA TGATGAAGCT CGCAAATGGT CCAGCAAGCC GGGCATCCCA
GCCGCTGAGG TGGGTATAGG TGTTGTAGCT GAGGCAGATG CGGCCGATGC TGCTGGCTTC
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCCT TGCATCGGGA AGCTACCAAG
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GCTGATGGCT
GAGATGTCTC GGCTGGTAAG AGGGGGCAGT GGTACCAAGC GGGCACTCAT TCAGTGTGCC
AAGGACATCG CCAAGGCCTC AGATGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG
TGCACAGATA AACGGATTAG AACCAACCTC TTACAGGTAT GTGAGCGAAT CCCAACCATA
AGCACCCAGC TCAAAATCCT GTCCACAGTG AAGGCCACCA TGCTGGGCCG GACCAACATC
AGTGATGAGG AGTCTGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAACCTCATG
CAGTCTGTGA AGGAGACTGT GCGGGAAGCT GAAGCTGCTT CAATCAAAAT TCGAACAGAT
GCTGGATTTA CACTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005270199.1
CDS131..3538
Misc Feature(1)137..3535(+)
Translation

Target ORF information:

RefSeq Version XM_005270142
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005270142

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG
GCCAAAGGTT GGCTCCGTGA CCCTAGTGCC TCCCCAGGGG ATGCTGGTGA GCAGGCCATC
AGACAGATCT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG
GAGATTCTGG GAACTTGCAA AATGCTAGGG CAGATGACTG ATCAAGTGGC TGACCTCCGT
GCCAGAGGAC AAGGATCCTC ACCGGTGGCC ATGCAGAAAG CTCAGCAGGT ATCTCAGGGT
CTGGATGTGC TCACAGCAAA AGTGGAAAAT GCAGCTCGCA AGCTGGAAGC CATGACCAAC
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ACTGGCTTGC AGATCCAAAT
GGTGGACCGG AAGGAGAAGA GCAGATTCGA GGTGCTTTGG CTGAAGCTCG GAAAATAGCA
GAATTATGTG ATGATCCTAA AGAAAGAGAT GACATTCTAC GTTCCCTTGG GGAAATATCT
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGGA AAGGAGATTC TCCAGAGGCT
CGAGCCTTGG CCAAACAGGT GGCCACGGCC CTGCAGAACC TGCAGACCAA AACCAACCGG
GCTGTGGCCA ACAGCAGACC GGCCAAAGCA GCTGTACACC TTGAGGGCAA GATTGAGCAA
GCACAGCGGT GGATTGATAA TCCCACAGTG GATGACCGTG GAGTCGGTCA GGCTGCCATC
CGGGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTTA TGATGGGGCC TTATCGGCAA
GATCTTCTCG CCAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCA CGAGCACTTG CATCTCAGCT CCAAGACTCC
TTAAAGGATC TAAAAGCTCG GATGCAGGAG GCCATGACTC AGGAAGTGTC AGATGTTTTC
AGCGATACCA CAACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACGGCGCC TCCTGATGCG
CCTAACAGGG AAGAGGTATT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAAG
CTTGGTGCTA CGGCCGAGAA GGCGGCTGCG GTTGGTACTG CTAATAAATC AACAGTGGAA
GGCATTCAGG CCTCAGTGAA GACGGCCCGA GAACTCACAC CCCAGGTGGT CTCGGCTGCT
CGTATCTTAC TTAGGAACCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGATACC
AAATCTCTGT TGGATGCTTC AGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGCAAGGTA
GCTATGGCCA ACATTCAGCC TCAGATGCTG GTTGCTGGGG CAACCAGTAT TGCTCGTCGG
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ATTCCGAGGA TCCCAAGTTC
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC GATGGTGATG
GATGCAAAAG CTGTGGCTGG AAACATTTCC GACCCTGGAC TGCAAAAGAG CTTCCTGGAC
TCAGGATATC GGATCCTGGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCTCAGGAG
CCTGACTTCC CGCCGCCTCC ACCAGACCTT GAACAACTCC GACTAACAGA TGAGCTTGCT
CCTCCCAAAC CACCTCTGCC TGAAGGTGAG GTCCCTCCAC CTAGGCCTCC ACCACCAGAG
GAAAAGGATG AAGAGTTCCC TGAGCAGAAG GCCGGGGAGG TGATTAACCA GCCAATGATG
ATGGCTGCCA GACAGCTCCA TGATGAAGCT CGCAAATGGT CCAGCAAGCC GGGCATCCCA
GCCGCTGAGG TGGGTATAGG TGTTGTAGCT GAGGCAGATG CGGCCGATGC TGCTGGCTTC
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCCT TGCATCGGGA AGCTACCAAG
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GCTGATGGCT
GAGATGTCTC GGCTGGTAAG AGGGGGCAGT GGTACCAAGC GGGCACTCAT TCAGTGTGCC
AAGGACATCG CCAAGGCCTC AGATGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG
TGCACAGATA AACGGATTAG AACCAACCTC TTACAGGTAT GTGAGCGAAT CCCAACCATA
AGCACCCAGC TCAAAATCCT GTCCACAGTG AAGGCCACCA TGCTGGGCCG GACCAACATC
AGTGATGAGG AGTCTGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAACCTCATG
CAGTCTGTGA AGGAGACTGT GCGGGAAGCT GAAGCTGCTT CAATCAAAAT TCGAACAGAT
GCTGGATTTA CACTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu44533
Clone ID Related Accession (Same CDS sequence) XM_005270143
Accession Version XM_005270143.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product vinculin isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG
GCCAAAGGTT GGCTCCGTGA CCCTAGTGCC TCCCCAGGGG ATGCTGGTGA GCAGGCCATC
AGACAGATCT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG
GAGATTCTGG GAACTTGCAA AATGCTAGGG CAGATGACTG ATCAAGTGGC TGACCTCCGT
GCCAGAGGAC AAGGATCCTC ACCGGTGGCC ATGCAGAAAG CTCAGCAGGT ATCTCAGGGT
CTGGATGTGC TCACAGCAAA AGTGGAAAAT GCAGCTCGCA AGCTGGAAGC CATGACCAAC
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ACTGGCTTGC AGATCCAAAT
GGTGGACCGG AAGGAGAAGA GCAGATTCGA GGTGCTTTGG CTGAAGCTCG GAAAATAGCA
GAATTATGTG ATGATCCTAA AGAAAGAGAT GACATTCTAC GTTCCCTTGG GGAAATATCT
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGGA AAGGAGATTC TCCAGAGGCT
CGAGCCTTGG CCAAACAGGT GGCCACGGCC CTGCAGAACC TGCAGACCAA AACCAACCGG
GCTGTGGCCA ACAGCAGACC GGCCAAAGCA GCTGTACACC TTGAGGGCAA GATTGAGCAA
GCACAGCGGT GGATTGATAA TCCCACAGTG GATGACCGTG GAGTCGGTCA GGCTGCCATC
CGGGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTTA TGATGGGGCC TTATCGGCAA
GATCTTCTCG CCAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCA CGAGCACTTG CATCTCAGCT CCAAGACTCC
TTAAAGGATC TAAAAGCTCG GATGCAGGAG GCCATGACTC AGGAAGTGTC AGATGTTTTC
AGCGATACCA CAACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACGGCGCC TCCTGATGCG
CCTAACAGGG AAGAGGTATT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAAG
CTTGGTGCTA CGGCCGAGAA GGCGGCTGCG GTTGGTACTG CTAATAAATC AACAGTGGAA
GGCATTCAGG CCTCAGTGAA GACGGCCCGA GAACTCACAC CCCAGGTGGT CTCGGCTGCT
CGTATCTTAC TTAGGAACCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGATACC
AAATCTCTGT TGGATGCTTC AGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGCAAGGTA
GCTATGGCCA ACATTCAGCC TCAGATGCTG GTTGCTGGGG CAACCAGTAT TGCTCGTCGG
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ATTCCGAGGA TCCCAAGTTC
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC GATGGTGATG
GATGCAAAAG CTGTGGCTGG AAACATTTCC GACCCTGGAC TGCAAAAGAG CTTCCTGGAC
TCAGGATATC GGATCCTGGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCTCAGGAG
CCTGACTTCC CGCCGCCTCC ACCAGACCTT GAACAACTCC GACTAACAGA TGAGCTTGCT
CCTCCCAAAC CACCTCTGCC TGAAGGTGAG GTCCCTCCAC CTAGGCCTCC ACCACCAGAG
GAAAAGGATG AAGAGTTCCC TGAGCAGAAG GCCGGGGAGG TGATTAACCA GCCAATGATG
ATGGCTGCCA GACAGCTCCA TGATGAAGCT CGCAAATGGT CCAGCAAGGG CAATGACATC
ATTGCAGCAG CCAAGCGCAT GGCTCTGCTG ATGGCTGAGA TGTCTCGGCT GGTAAGAGGG
GGCAGTGGTA CCAAGCGGGC ACTCATTCAG TGTGCCAAGG ACATCGCCAA GGCCTCAGAT
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAACG GATTAGAACC
AACCTCTTAC AGGTATGTGA GCGAATCCCA ACCATAAGCA CCCAGCTCAA AATCCTGTCC
ACAGTGAAGG CCACCATGCT GGGCCGGACC AACATCAGTG ATGAGGAGTC TGAGCAGGCC
ACAGAGATGC TGGTTCACAA TGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACTGTGCGG
GAAGCTGAAG CTGCTTCAAT CAAAATTCGA ACAGATGCTG GATTTACACT GCGCTGGGTT
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005270200.1
CDS131..3334
Misc Feature(1)137..3331(+)
Translation

Target ORF information:

RefSeq Version XM_005270143
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005270143

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG
GCCAAAGGTT GGCTCCGTGA CCCTAGTGCC TCCCCAGGGG ATGCTGGTGA GCAGGCCATC
AGACAGATCT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG
GAGATTCTGG GAACTTGCAA AATGCTAGGG CAGATGACTG ATCAAGTGGC TGACCTCCGT
GCCAGAGGAC AAGGATCCTC ACCGGTGGCC ATGCAGAAAG CTCAGCAGGT ATCTCAGGGT
CTGGATGTGC TCACAGCAAA AGTGGAAAAT GCAGCTCGCA AGCTGGAAGC CATGACCAAC
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ACTGGCTTGC AGATCCAAAT
GGTGGACCGG AAGGAGAAGA GCAGATTCGA GGTGCTTTGG CTGAAGCTCG GAAAATAGCA
GAATTATGTG ATGATCCTAA AGAAAGAGAT GACATTCTAC GTTCCCTTGG GGAAATATCT
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGGA AAGGAGATTC TCCAGAGGCT
CGAGCCTTGG CCAAACAGGT GGCCACGGCC CTGCAGAACC TGCAGACCAA AACCAACCGG
GCTGTGGCCA ACAGCAGACC GGCCAAAGCA GCTGTACACC TTGAGGGCAA GATTGAGCAA
GCACAGCGGT GGATTGATAA TCCCACAGTG GATGACCGTG GAGTCGGTCA GGCTGCCATC
CGGGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTTA TGATGGGGCC TTATCGGCAA
GATCTTCTCG CCAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCA CGAGCACTTG CATCTCAGCT CCAAGACTCC
TTAAAGGATC TAAAAGCTCG GATGCAGGAG GCCATGACTC AGGAAGTGTC AGATGTTTTC
AGCGATACCA CAACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACGGCGCC TCCTGATGCG
CCTAACAGGG AAGAGGTATT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAAG
CTTGGTGCTA CGGCCGAGAA GGCGGCTGCG GTTGGTACTG CTAATAAATC AACAGTGGAA
GGCATTCAGG CCTCAGTGAA GACGGCCCGA GAACTCACAC CCCAGGTGGT CTCGGCTGCT
CGTATCTTAC TTAGGAACCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGATACC
AAATCTCTGT TGGATGCTTC AGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGCAAGGTA
GCTATGGCCA ACATTCAGCC TCAGATGCTG GTTGCTGGGG CAACCAGTAT TGCTCGTCGG
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ATTCCGAGGA TCCCAAGTTC
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC GATGGTGATG
GATGCAAAAG CTGTGGCTGG AAACATTTCC GACCCTGGAC TGCAAAAGAG CTTCCTGGAC
TCAGGATATC GGATCCTGGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCTCAGGAG
CCTGACTTCC CGCCGCCTCC ACCAGACCTT GAACAACTCC GACTAACAGA TGAGCTTGCT
CCTCCCAAAC CACCTCTGCC TGAAGGTGAG GTCCCTCCAC CTAGGCCTCC ACCACCAGAG
GAAAAGGATG AAGAGTTCCC TGAGCAGAAG GCCGGGGAGG TGATTAACCA GCCAATGATG
ATGGCTGCCA GACAGCTCCA TGATGAAGCT CGCAAATGGT CCAGCAAGGG CAATGACATC
ATTGCAGCAG CCAAGCGCAT GGCTCTGCTG ATGGCTGAGA TGTCTCGGCT GGTAAGAGGG
GGCAGTGGTA CCAAGCGGGC ACTCATTCAG TGTGCCAAGG ACATCGCCAA GGCCTCAGAT
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAACG GATTAGAACC
AACCTCTTAC AGGTATGTGA GCGAATCCCA ACCATAAGCA CCCAGCTCAA AATCCTGTCC
ACAGTGAAGG CCACCATGCT GGGCCGGACC AACATCAGTG ATGAGGAGTC TGAGCAGGCC
ACAGAGATGC TGGTTCACAA TGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACTGTGCGG
GAAGCTGAAG CTGCTTCAAT CAAAATTCGA ACAGATGCTG GATTTACACT GCGCTGGGTT
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25616
Clone ID Related Accession (Same CDS sequence) NM_014000
Accession Version NM_014000.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product vinculin isoform meta-VCL
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL708715.1, BC039174.1, AK126978.1, AL832130.1, AA368699.1, AL700886.1, BC013847.1 and AI290052.1. This sequence is a reference standard in the RefSeqGene project. On Jul 26, 2004 this sequence version replaced gi:7669549. Transcript Variant: This variant (1) differs from variant 2 in having an additional exon in the 3' coding region. It thus encodes a longer isoform (meta-VCL). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG CATCCCAGCC
GCTGAGGTGG GTATAGGTGT TGTAGCTGAG GCAGATGCGG CCGATGCTGC TGGCTTCCCT
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ATCGGGAAGC TACCAAGTGG
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCTGAG
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTCATTCA GTGTGCCAAG
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC
ACAGATAAAC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC AACCATAAGC
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG
TCTGTGAAGG AGACTGTGCG GGAAGCTGAA GCTGCTTCAA TCAAAATTCG AACAGATGCT
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_054706.1
CDS95..3499
Misc Feature(1)2..4(+)
Misc Feature(2)98..2599(+)
Misc Feature(3)101..3496(+)
Misc Feature(4)347..349(+)
Misc Feature(5)392..394(+)
Misc Feature(6)596..718(+)
Misc Feature(7)611..613(+)
Misc Feature(8)869..1861(+)
Misc Feature(9)956..958(+)
Misc Feature(10)956..958(+)
Misc Feature(11)962..964(+)
Misc Feature(12)962..964(+)
Misc Feature(13)1130..1132(+)
Misc Feature(14)1394..1396(+)
Misc Feature(15)1580..1582(+)
Misc Feature(16)1904..1906(+)
Misc Feature(17)2168..2170(+)
Misc Feature(18)2255..2257(+)
Misc Feature(19)2255..2257(+)
Misc Feature(20)2558..2560(+)
Misc Feature(21)2558..2560(+)
Misc Feature(22)2600..2728(+)
Misc Feature(23)2729..3496(+)
Misc Feature(24)3101..3232(+)
Misc Feature(25)3395..3397(+)
Misc Feature(26)3431..3433(+)
Misc Feature(27)3452..3496(+)
Misc Feature(28)3491..3493(+)
Misc Feature(29)3491..3493(+)
Exon (1)1..262
Gene:VCL
Gene Synonym:
Exon (2)263..333
Gene:VCL
Gene Synonym:
Exon (3)334..484
Gene:VCL
Gene Synonym:
Exon (4)485..593
Gene:VCL
Gene Synonym:
Exon (5)594..716
Gene:VCL
Gene Synonym:
Exon (6)717..877
Gene:VCL
Gene Synonym:
Exon (7)878..968
Gene:VCL
Gene Synonym:
Exon (8)969..1116
Gene:VCL
Gene Synonym:
Exon (9)1117..1270
Gene:VCL
Gene Synonym:
Exon (10)1271..1446
Gene:VCL
Gene Synonym:
Exon (11)1447..1637
Gene:VCL
Gene Synonym:
Exon (12)1638..1837
Gene:VCL
Gene Synonym:
Exon (13)1838..1966
Gene:VCL
Gene Synonym:
Exon (14)1967..2116
Gene:VCL
Gene Synonym:
Exon (15)2117..2225
Gene:VCL
Gene Synonym:
Exon (16)2226..2528
Gene:VCL
Gene Synonym:
Exon (17)2529..2653
Gene:VCL
Gene Synonym:
Exon (18)2654..2839
Gene:VCL
Gene Synonym:
Exon (19)2840..3043
Gene:VCL
Gene Synonym:
Exon (20)3044..3247
Gene:VCL
Gene Synonym:
Exon (21)3248..3352
Gene:VCL
Gene Synonym:
Exon (22)3353..5490
Gene:VCL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_014000
Organism Homo sapiens (human)
Definition Homo sapiens vinculin (VCL), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014000

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG CATCCCAGCC
GCTGAGGTGG GTATAGGTGT TGTAGCTGAG GCAGATGCGG CCGATGCTGC TGGCTTCCCT
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ATCGGGAAGC TACCAAGTGG
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCTGAG
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTCATTCA GTGTGCCAAG
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC
ACAGATAAAC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC AACCATAAGC
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG
TCTGTGAAGG AGACTGTGCG GGAAGCTGAA GCTGCTTCAA TCAAAATTCG AACAGATGCT
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25331
Clone ID Related Accession (Same CDS sequence) NM_003373
Accession Version NM_003373.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product vinculin isoform VCL
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL708715.1, BC039174.1, BX537994.1, AK126978.1, BC013847.1, AI290052.1 and AL832130.1. On Jul 26, 2004 this sequence version replaced gi:7669551. Transcript Variant: This variant (2) lacks a 3' coding exon, as compared to variant 1. It encodes a shorter isoform (VCL), which is the major talin-binding protein in platelets. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC039174.1, M33308.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC
AGTGGTACCA AGCGGGCACT CATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAACGGAT TAGAACCAAC
CTCTTACAGG TATGTGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA
GCTGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003364.1
CDS95..3295
Misc Feature(1)2..4(+)
Misc Feature(2)101..3292(+)
Misc Feature(3)347..349(+)
Misc Feature(4)392..394(+)
Misc Feature(5)956..958(+)
Misc Feature(6)962..964(+)
Misc Feature(7)1904..1906(+)
Misc Feature(8)2168..2170(+)
Misc Feature(9)2255..2257(+)
Misc Feature(10)2558..2560(+)
Misc Feature(11)3191..3193(+)
Misc Feature(12)3227..3229(+)
Misc Feature(13)3287..3289(+)
Exon (1)1..262
Gene:VCL
Gene Synonym:
Exon (2)263..333
Gene:VCL
Gene Synonym:
Exon (3)334..484
Gene:VCL
Gene Synonym:
Exon (4)485..593
Gene:VCL
Gene Synonym:
Exon (5)594..716
Gene:VCL
Gene Synonym:
Exon (6)717..877
Gene:VCL
Gene Synonym:
Exon (7)878..968
Gene:VCL
Gene Synonym:
Exon (8)969..1116
Gene:VCL
Gene Synonym:
Exon (9)1117..1270
Gene:VCL
Gene Synonym:
Exon (10)1271..1446
Gene:VCL
Gene Synonym:
Exon (11)1447..1637
Gene:VCL
Gene Synonym:
Exon (12)1638..1837
Gene:VCL
Gene Synonym:
Exon (13)1838..1966
Gene:VCL
Gene Synonym:
Exon (14)1967..2116
Gene:VCL
Gene Synonym:
Exon (15)2117..2225
Gene:VCL
Gene Synonym:
Exon (16)2226..2528
Gene:VCL
Gene Synonym:
Exon (17)2529..2653
Gene:VCL
Gene Synonym:
Exon (18)2654..2839
Gene:VCL
Gene Synonym:
Exon (19)2840..3043
Gene:VCL
Gene Synonym:
Exon (20)3044..3148
Gene:VCL
Gene Synonym:
Exon (21)3149..5286
Gene:VCL
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003373
Organism Homo sapiens (human)
Definition Homo sapiens vinculin (VCL), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003373

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC
AGTGGTACCA AGCGGGCACT CATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAACGGAT TAGAACCAAC
CTCTTACAGG TATGTGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA
GCTGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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