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WFS1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol WFS1
Entrez Gene ID 7466
Full Name Wolfram syndrome 1 (wolframin)
Synonyms CTRCT41, WFRS, WFS, WFSL
General protein information
Preferred Names
wolframin
Names
wolframin
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4p16.1

Summary This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]. lac of sum
Disorder MIM:

606201

Disorder Html: Wolfram syndrome, 222300 (3); Deafness, autosomal dominant 6/14/38,

mRNA and Protein(s)

mRNA Protein Name
NM_006005 NP_005996 wolframin
NM_001145853 NP_001139325 wolframin



Homo sapiens (human) WFS1 NP_001139325.1
Pan troglodytes (chimpanzee) WFS1 XP_003310288.1
Macaca mulatta (Rhesus monkey) WFS1 XP_001092811.1
Canis lupus familiaris (dog) WFS1 XP_539234.4
Bos taurus (cattle) WFS1 XP_002688446.1
Mus musculus (house mouse) Wfs1 NP_035846.1
Rattus norvegicus (Norway rat) Wfs1 NP_114011.1
Gallus gallus (chicken) WFS1 XP_420803.2
Danio rerio (zebrafish) wfs1b XP_005157280.1
Drosophila melanogaster (fruit fly) wfs1 NP_001189267.1
Xenopus (Silurana) tropicalis (western clawed frog) wfs1 NP_001106459.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following WFS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WFS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26100
NM_006005 Homo sapiens Wolfram syndrome 1 (wolframin) (WFS1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu26100
NM_001145853 Homo sapiens Wolfram syndrome 1 (wolframin) (WFS1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26100
Clone ID Related Accession (Same CDS sequence) NM_001145853 , NM_006005
Accession Version NM_006005.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2673bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product wolframin
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF084481.1, BC069213.1 and Y18064.1. This sequence is a reference standard in the RefSeqGene project. On Mar 14, 2009 this sequence version replaced gi:13376995. Transcript Variant: This variant (1) is the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF084481.1, AK312897.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGACTCCA ACACTGCTCC GCTGGGCCCC TCCTGCCCAC AGCCCCCGCC AGCACCGCAG 
CCCCAGGCGC GTTCCCGACT CAATGCCACA GCCTCGTTGG AGCAGGAGAG GAGCGAAAGG
CCCCGAGCAC CCGGACCCCA GGCTGGCCCT GGCCCTGGTG TTAGAGACGC AGCGGCCCCC
GCTGAACCCC AGGCCCAGCA TACCAGGAGC CGGGAAAGAG CAGACGGCAC CGGGCCTACA
AAGGGAGACA TGGAAATCCC CTTTGAAGAA GTCCTGGAGA GGGCCAAGGC CGGGGACCCC
AAGGCACAGA CTGAGGTGGG GAAGCACTAC CTGCAGTTGG CCGGCGACAC GGATGAAGAA
CTCAACAGCT GCACCGCTGT GGACTGGCTG GTCCTCGCCG CGAAGCAGGG CCGTCGCGAG
GCTGTGAAGC TGCTTCGCCG GTGCTTGGCG GACAGAAGAG GCATCACGTC CGAGAACGAA
CGGGAGGTGA GGCAGCTCTC CTCCGAGACC GACCTGGAGA GGGCCGTGCG CAAGGCAGCC
CTGGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAGAAGC AGGTGGCCGT GGCGGAGCTG
CTGGAGAATG TCGGCCAGGT CAACGAGCAC GATGGAGGGG CGCAGCCAGG CCCCGTGCCC
AAGTCCCTGC AGAAGCAGAG GCGCATGCTG GAGCGCCTGG TCAGCAGCGA GTCCAAGAAC
TACATCGCGC TGGATGACTT TGTGGAGATC ACTAAGAAGT ACGCCAAGGG CGTCATCCCC
AGCAGCCTGT TCCTGCAGGA CGACGAAGAT GATGACGAGC TGGCGGGGAA GAGCCCTGAG
GACCTGCCAC TGCGTCTGAA GGTGGTCAAG TACCCCCTGC ACGCCATCAT GGAGATCAAG
GAGTACCTGA TTGACATGGC CTCCAGGGCA GGCATGCACT GGCTGTCCAC CATCATCCCC
ACGCACCACA TCAACGCGCT CATCTTCTTC TTCATCGTCA GCAACCTCAC CATCGACTTC
TTCGCCTTCT TCATCCCGCT GGTCATCTTC TACCTGTCCT TCATCTCCAT GGTGATCTGC
ACCCTCAAGG TGTTCCAGGA CAGCAAGGCC TGGGAGAACT TCCGCACCCT CACCGACCTG
CTGCTGCGCT TCGAGCCCAA CCTGGATGTG GAGCAGGCCG AGGTCAACTT CGGCTGGAAC
CACCTGGAGC CCTATGCCCA TTTCCTGCTC TCTGTCTTCT TCGTCATCTT CTCCTTCCCC
ATCGCCAGCA AGGACTGCAT CCCCTGCTCG GAGCTGGCTG TCATCACCGG CTTCTTTACC
GTGACCAGCT ACCTGAGCCT GAGCACCCAT GCAGAGCCCT ACACGCGCAG GGCCCTGGCC
ACCGAGGTCA CCGCCGGCCT GCTATCGCTG CTGCCCTCCA TGCCCTTGAA TTGGCCCTAC
CTGAAGGTCC TTGGCCAGAC CTTCATCACC GTGCCTGTCG GCCACCTGGT CGTCCTCAAC
GTCAGCGTCC CGTGCCTGCT CTATGTCTAC CTGCTCTATC TCTTCTTCCG CATGGCACAG
CTGAGGAATT TCAAGGGCAC CTACTGCTAC CTTGTGCCCT ACCTGGTGTG CTTCATGTGG
TGTGAGCTCT CCGTGGTCAT CCTGCTGGAG TCCACCGGCC TGGGGCTGCT CCGCGCCTCC
ATCGGCTACT TCCTCTTCCT CTTTGCCCTC CCCATCCTGG TGGCCGGCCT GGCCCTGGTG
GGCGTGCTGC AGTTCGCCCG GTGGTTCACG TCTCTGGAGC TCACCAAGAT CGCAGTCACC
GTGGCGGTCT GTAGTGTGCC CCTGCTGTTG CGCTGGTGGA CCAAGGCCAG CTTCTCTGTG
GTGGGGATGG TGAAGTCCCT GACGCGGAGC TCCATGGTCA AGCTCATCCT GGTGTGGCTC
ACGGCCATCG TGCTGTTCTG CTGGTTCTAT GTGTACCGCT CAGAGGGCAT GAAGGTCTAC
AACTCCACAC TGACCTGGCA GCAGTATGGT GCGCTGTGCG GGCCACGCGC CTGGAAGGAG
ACCAACATGG CGCGCACCCA GATCCTCTGC AGCCACCTGG AGGGCCACAG GGTCACGTGG
ACCGGCCGCT TCAAGTACGT CCGCGTGACT GACATCGACA ACAGCGCCGA GTCTGCCATC
AACATGCTCC CGTTCTTCAT CGGCGACTGG ATGCGCTGCC TCTACGGCGA GGCCTACCCT
GCCTGCAGCC CTGGCAACAC CTCCACGGCC GAGGAGGAGC TCTGTCGCCT TAAGCTGCTG
GCCAAGCACC CCTGCCACAT CAAGAAGTTC GACCGCTACA AGTTTGAGAT TACCGTGGGC
ATGCCATTCA GCAGCGGCGC TGACGGCTCG CGCAGCCGCG AGGAGGACGA CGTCACCAAG
GACATCGTGC TGCGGGCCAG CAGCGAGTTC AAGAGCGTGC TGCTCAGCCT GCGCCAGGGC
AGCCTCATCG AGTTCAGCAC CATCCTGGAG GGCCGCCTGG GCAGCAAGTG GCCTGTCTTC
GAGCTCAAGG CCATCAGCTG CCTCAACTGC ATGGCCCAGC TCTCACCCAC CAGGCGGCAC
GTGAAGATCG AGCACGACTG GCGCAGCACC GTGCATGGCG CCGTGAAGTT CGCCTTCGAC
TTCTTTTTCT TCCCATTCCT GTCGGCGGCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005996.2
CDS171..2843
Misc Feature(1)171..173(+)
Misc Feature(2)1110..1172(+)
Misc Feature(3)1188..1250(+)
Misc Feature(4)1374..1436(+)
Misc Feature(5)1449..1511(+)
Misc Feature(6)1563..1625(+)
Misc Feature(7)1656..1718(+)
Misc Feature(8)1755..1817(+)
Misc Feature(9)1857..1919(+)
Misc Feature(10)1935..1997(+)
Misc Feature(11)2064..2126(+)
Misc Feature(12)2778..2840(+)
Exon (1)1..165
Gene:WFS1
Gene Synonym:
Exon (2)166..402
Gene:WFS1
Gene Synonym:
Exon (3)403..485
Gene:WFS1
Gene Synonym:
Exon (4)486..630
Gene:WFS1
Gene Synonym:
Exon (5)631..801
Gene:WFS1
Gene Synonym:
Exon (6)802..882
Gene:WFS1
Gene Synonym:
Exon (7)883..1031
Gene:WFS1
Gene Synonym:
Exon (8)1032..3640
Gene:WFS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006005
Organism Homo sapiens (human)
Definition Homo sapiens Wolfram syndrome 1 (wolframin) (WFS1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006005

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGACTCCA ACACTGCTCC GCTGGGCCCC TCCTGCCCAC AGCCCCCGCC AGCACCGCAG 
CCCCAGGCGC GTTCCCGACT CAATGCCACA GCCTCGTTGG AGCAGGAGAG GAGCGAAAGG
CCCCGAGCAC CCGGACCCCA GGCTGGCCCT GGCCCTGGTG TTAGAGACGC AGCGGCCCCC
GCTGAACCCC AGGCCCAGCA TACCAGGAGC CGGGAAAGAG CAGACGGCAC CGGGCCTACA
AAGGGAGACA TGGAAATCCC CTTTGAAGAA GTCCTGGAGA GGGCCAAGGC CGGGGACCCC
AAGGCACAGA CTGAGGTGGG GAAGCACTAC CTGCAGTTGG CCGGCGACAC GGATGAAGAA
CTCAACAGCT GCACCGCTGT GGACTGGCTG GTCCTCGCCG CGAAGCAGGG CCGTCGCGAG
GCTGTGAAGC TGCTTCGCCG GTGCTTGGCG GACAGAAGAG GCATCACGTC CGAGAACGAA
CGGGAGGTGA GGCAGCTCTC CTCCGAGACC GACCTGGAGA GGGCCGTGCG CAAGGCAGCC
CTGGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAGAAGC AGGTGGCCGT GGCGGAGCTG
CTGGAGAATG TCGGCCAGGT CAACGAGCAC GATGGAGGGG CGCAGCCAGG CCCCGTGCCC
AAGTCCCTGC AGAAGCAGAG GCGCATGCTG GAGCGCCTGG TCAGCAGCGA GTCCAAGAAC
TACATCGCGC TGGATGACTT TGTGGAGATC ACTAAGAAGT ACGCCAAGGG CGTCATCCCC
AGCAGCCTGT TCCTGCAGGA CGACGAAGAT GATGACGAGC TGGCGGGGAA GAGCCCTGAG
GACCTGCCAC TGCGTCTGAA GGTGGTCAAG TACCCCCTGC ACGCCATCAT GGAGATCAAG
GAGTACCTGA TTGACATGGC CTCCAGGGCA GGCATGCACT GGCTGTCCAC CATCATCCCC
ACGCACCACA TCAACGCGCT CATCTTCTTC TTCATCGTCA GCAACCTCAC CATCGACTTC
TTCGCCTTCT TCATCCCGCT GGTCATCTTC TACCTGTCCT TCATCTCCAT GGTGATCTGC
ACCCTCAAGG TGTTCCAGGA CAGCAAGGCC TGGGAGAACT TCCGCACCCT CACCGACCTG
CTGCTGCGCT TCGAGCCCAA CCTGGATGTG GAGCAGGCCG AGGTCAACTT CGGCTGGAAC
CACCTGGAGC CCTATGCCCA TTTCCTGCTC TCTGTCTTCT TCGTCATCTT CTCCTTCCCC
ATCGCCAGCA AGGACTGCAT CCCCTGCTCG GAGCTGGCTG TCATCACCGG CTTCTTTACC
GTGACCAGCT ACCTGAGCCT GAGCACCCAT GCAGAGCCCT ACACGCGCAG GGCCCTGGCC
ACCGAGGTCA CCGCCGGCCT GCTATCGCTG CTGCCCTCCA TGCCCTTGAA TTGGCCCTAC
CTGAAGGTCC TTGGCCAGAC CTTCATCACC GTGCCTGTCG GCCACCTGGT CGTCCTCAAC
GTCAGCGTCC CGTGCCTGCT CTATGTCTAC CTGCTCTATC TCTTCTTCCG CATGGCACAG
CTGAGGAATT TCAAGGGCAC CTACTGCTAC CTTGTGCCCT ACCTGGTGTG CTTCATGTGG
TGTGAGCTCT CCGTGGTCAT CCTGCTGGAG TCCACCGGCC TGGGGCTGCT CCGCGCCTCC
ATCGGCTACT TCCTCTTCCT CTTTGCCCTC CCCATCCTGG TGGCCGGCCT GGCCCTGGTG
GGCGTGCTGC AGTTCGCCCG GTGGTTCACG TCTCTGGAGC TCACCAAGAT CGCAGTCACC
GTGGCGGTCT GTAGTGTGCC CCTGCTGTTG CGCTGGTGGA CCAAGGCCAG CTTCTCTGTG
GTGGGGATGG TGAAGTCCCT GACGCGGAGC TCCATGGTCA AGCTCATCCT GGTGTGGCTC
ACGGCCATCG TGCTGTTCTG CTGGTTCTAT GTGTACCGCT CAGAGGGCAT GAAGGTCTAC
AACTCCACAC TGACCTGGCA GCAGTATGGT GCGCTGTGCG GGCCACGCGC CTGGAAGGAG
ACCAACATGG CGCGCACCCA GATCCTCTGC AGCCACCTGG AGGGCCACAG GGTCACGTGG
ACCGGCCGCT TCAAGTACGT CCGCGTGACT GACATCGACA ACAGCGCCGA GTCTGCCATC
AACATGCTCC CGTTCTTCAT CGGCGACTGG ATGCGCTGCC TCTACGGCGA GGCCTACCCT
GCCTGCAGCC CTGGCAACAC CTCCACGGCC GAGGAGGAGC TCTGTCGCCT TAAGCTGCTG
GCCAAGCACC CCTGCCACAT CAAGAAGTTC GACCGCTACA AGTTTGAGAT TACCGTGGGC
ATGCCATTCA GCAGCGGCGC TGACGGCTCG CGCAGCCGCG AGGAGGACGA CGTCACCAAG
GACATCGTGC TGCGGGCCAG CAGCGAGTTC AAGAGCGTGC TGCTCAGCCT GCGCCAGGGC
AGCCTCATCG AGTTCAGCAC CATCCTGGAG GGCCGCCTGG GCAGCAAGTG GCCTGTCTTC
GAGCTCAAGG CCATCAGCTG CCTCAACTGC ATGGCCCAGC TCTCACCCAC CAGGCGGCAC
GTGAAGATCG AGCACGACTG GCGCAGCACC GTGCATGGCG CCGTGAAGTT CGCCTTCGAC
TTCTTTTTCT TCCCATTCCT GTCGGCGGCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26100
Clone ID Related Accession (Same CDS sequence) NM_001145853 , NM_006005
Accession Version NM_001145853.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2673bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product wolframin
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF084481.1, BC030130.2 and Y18064.1. Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, resulting in a slightly shorter transcript, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC030130.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGACTCCA ACACTGCTCC GCTGGGCCCC TCCTGCCCAC AGCCCCCGCC AGCACCGCAG 
CCCCAGGCGC GTTCCCGACT CAATGCCACA GCCTCGTTGG AGCAGGAGAG GAGCGAAAGG
CCCCGAGCAC CCGGACCCCA GGCTGGCCCT GGCCCTGGTG TTAGAGACGC AGCGGCCCCC
GCTGAACCCC AGGCCCAGCA TACCAGGAGC CGGGAAAGAG CAGACGGCAC CGGGCCTACA
AAGGGAGACA TGGAAATCCC CTTTGAAGAA GTCCTGGAGA GGGCCAAGGC CGGGGACCCC
AAGGCACAGA CTGAGGTGGG GAAGCACTAC CTGCAGTTGG CCGGCGACAC GGATGAAGAA
CTCAACAGCT GCACCGCTGT GGACTGGCTG GTCCTCGCCG CGAAGCAGGG CCGTCGCGAG
GCTGTGAAGC TGCTTCGCCG GTGCTTGGCG GACAGAAGAG GCATCACGTC CGAGAACGAA
CGGGAGGTGA GGCAGCTCTC CTCCGAGACC GACCTGGAGA GGGCCGTGCG CAAGGCAGCC
CTGGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAGAAGC AGGTGGCCGT GGCGGAGCTG
CTGGAGAATG TCGGCCAGGT CAACGAGCAC GATGGAGGGG CGCAGCCAGG CCCCGTGCCC
AAGTCCCTGC AGAAGCAGAG GCGCATGCTG GAGCGCCTGG TCAGCAGCGA GTCCAAGAAC
TACATCGCGC TGGATGACTT TGTGGAGATC ACTAAGAAGT ACGCCAAGGG CGTCATCCCC
AGCAGCCTGT TCCTGCAGGA CGACGAAGAT GATGACGAGC TGGCGGGGAA GAGCCCTGAG
GACCTGCCAC TGCGTCTGAA GGTGGTCAAG TACCCCCTGC ACGCCATCAT GGAGATCAAG
GAGTACCTGA TTGACATGGC CTCCAGGGCA GGCATGCACT GGCTGTCCAC CATCATCCCC
ACGCACCACA TCAACGCGCT CATCTTCTTC TTCATCGTCA GCAACCTCAC CATCGACTTC
TTCGCCTTCT TCATCCCGCT GGTCATCTTC TACCTGTCCT TCATCTCCAT GGTGATCTGC
ACCCTCAAGG TGTTCCAGGA CAGCAAGGCC TGGGAGAACT TCCGCACCCT CACCGACCTG
CTGCTGCGCT TCGAGCCCAA CCTGGATGTG GAGCAGGCCG AGGTCAACTT CGGCTGGAAC
CACCTGGAGC CCTATGCCCA TTTCCTGCTC TCTGTCTTCT TCGTCATCTT CTCCTTCCCC
ATCGCCAGCA AGGACTGCAT CCCCTGCTCG GAGCTGGCTG TCATCACCGG CTTCTTTACC
GTGACCAGCT ACCTGAGCCT GAGCACCCAT GCAGAGCCCT ACACGCGCAG GGCCCTGGCC
ACCGAGGTCA CCGCCGGCCT GCTATCGCTG CTGCCCTCCA TGCCCTTGAA TTGGCCCTAC
CTGAAGGTCC TTGGCCAGAC CTTCATCACC GTGCCTGTCG GCCACCTGGT CGTCCTCAAC
GTCAGCGTCC CGTGCCTGCT CTATGTCTAC CTGCTCTATC TCTTCTTCCG CATGGCACAG
CTGAGGAATT TCAAGGGCAC CTACTGCTAC CTTGTGCCCT ACCTGGTGTG CTTCATGTGG
TGTGAGCTCT CCGTGGTCAT CCTGCTGGAG TCCACCGGCC TGGGGCTGCT CCGCGCCTCC
ATCGGCTACT TCCTCTTCCT CTTTGCCCTC CCCATCCTGG TGGCCGGCCT GGCCCTGGTG
GGCGTGCTGC AGTTCGCCCG GTGGTTCACG TCTCTGGAGC TCACCAAGAT CGCAGTCACC
GTGGCGGTCT GTAGTGTGCC CCTGCTGTTG CGCTGGTGGA CCAAGGCCAG CTTCTCTGTG
GTGGGGATGG TGAAGTCCCT GACGCGGAGC TCCATGGTCA AGCTCATCCT GGTGTGGCTC
ACGGCCATCG TGCTGTTCTG CTGGTTCTAT GTGTACCGCT CAGAGGGCAT GAAGGTCTAC
AACTCCACAC TGACCTGGCA GCAGTATGGT GCGCTGTGCG GGCCACGCGC CTGGAAGGAG
ACCAACATGG CGCGCACCCA GATCCTCTGC AGCCACCTGG AGGGCCACAG GGTCACGTGG
ACCGGCCGCT TCAAGTACGT CCGCGTGACT GACATCGACA ACAGCGCCGA GTCTGCCATC
AACATGCTCC CGTTCTTCAT CGGCGACTGG ATGCGCTGCC TCTACGGCGA GGCCTACCCT
GCCTGCAGCC CTGGCAACAC CTCCACGGCC GAGGAGGAGC TCTGTCGCCT TAAGCTGCTG
GCCAAGCACC CCTGCCACAT CAAGAAGTTC GACCGCTACA AGTTTGAGAT TACCGTGGGC
ATGCCATTCA GCAGCGGCGC TGACGGCTCG CGCAGCCGCG AGGAGGACGA CGTCACCAAG
GACATCGTGC TGCGGGCCAG CAGCGAGTTC AAGAGCGTGC TGCTCAGCCT GCGCCAGGGC
AGCCTCATCG AGTTCAGCAC CATCCTGGAG GGCCGCCTGG GCAGCAAGTG GCCTGTCTTC
GAGCTCAAGG CCATCAGCTG CCTCAACTGC ATGGCCCAGC TCTCACCCAC CAGGCGGCAC
GTGAAGATCG AGCACGACTG GCGCAGCACC GTGCATGGCG CCGTGAAGTT CGCCTTCGAC
TTCTTTTTCT TCCCATTCCT GTCGGCGGCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001139325.1
CDS167..2839
Misc Feature(1)17..19(+)
Misc Feature(2)167..169(+)
Misc Feature(3)1106..1168(+)
Misc Feature(4)1184..1246(+)
Misc Feature(5)1370..1432(+)
Misc Feature(6)1445..1507(+)
Misc Feature(7)1559..1621(+)
Misc Feature(8)1652..1714(+)
Misc Feature(9)1751..1813(+)
Misc Feature(10)1853..1915(+)
Misc Feature(11)1931..1993(+)
Misc Feature(12)2060..2122(+)
Misc Feature(13)2774..2836(+)
Exon (1)1..165
Gene:WFS1
Gene Synonym:
Exon (2)166..398
Gene:WFS1
Gene Synonym:
Exon (3)399..481
Gene:WFS1
Gene Synonym:
Exon (4)482..626
Gene:WFS1
Gene Synonym:
Exon (5)627..797
Gene:WFS1
Gene Synonym:
Exon (6)798..878
Gene:WFS1
Gene Synonym:
Exon (7)879..1027
Gene:WFS1
Gene Synonym:
Exon (8)1028..3636
Gene:WFS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001145853
Organism Homo sapiens (human)
Definition Homo sapiens Wolfram syndrome 1 (wolframin) (WFS1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001145853

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGACTCCA ACACTGCTCC GCTGGGCCCC TCCTGCCCAC AGCCCCCGCC AGCACCGCAG 
CCCCAGGCGC GTTCCCGACT CAATGCCACA GCCTCGTTGG AGCAGGAGAG GAGCGAAAGG
CCCCGAGCAC CCGGACCCCA GGCTGGCCCT GGCCCTGGTG TTAGAGACGC AGCGGCCCCC
GCTGAACCCC AGGCCCAGCA TACCAGGAGC CGGGAAAGAG CAGACGGCAC CGGGCCTACA
AAGGGAGACA TGGAAATCCC CTTTGAAGAA GTCCTGGAGA GGGCCAAGGC CGGGGACCCC
AAGGCACAGA CTGAGGTGGG GAAGCACTAC CTGCAGTTGG CCGGCGACAC GGATGAAGAA
CTCAACAGCT GCACCGCTGT GGACTGGCTG GTCCTCGCCG CGAAGCAGGG CCGTCGCGAG
GCTGTGAAGC TGCTTCGCCG GTGCTTGGCG GACAGAAGAG GCATCACGTC CGAGAACGAA
CGGGAGGTGA GGCAGCTCTC CTCCGAGACC GACCTGGAGA GGGCCGTGCG CAAGGCAGCC
CTGGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAGAAGC AGGTGGCCGT GGCGGAGCTG
CTGGAGAATG TCGGCCAGGT CAACGAGCAC GATGGAGGGG CGCAGCCAGG CCCCGTGCCC
AAGTCCCTGC AGAAGCAGAG GCGCATGCTG GAGCGCCTGG TCAGCAGCGA GTCCAAGAAC
TACATCGCGC TGGATGACTT TGTGGAGATC ACTAAGAAGT ACGCCAAGGG CGTCATCCCC
AGCAGCCTGT TCCTGCAGGA CGACGAAGAT GATGACGAGC TGGCGGGGAA GAGCCCTGAG
GACCTGCCAC TGCGTCTGAA GGTGGTCAAG TACCCCCTGC ACGCCATCAT GGAGATCAAG
GAGTACCTGA TTGACATGGC CTCCAGGGCA GGCATGCACT GGCTGTCCAC CATCATCCCC
ACGCACCACA TCAACGCGCT CATCTTCTTC TTCATCGTCA GCAACCTCAC CATCGACTTC
TTCGCCTTCT TCATCCCGCT GGTCATCTTC TACCTGTCCT TCATCTCCAT GGTGATCTGC
ACCCTCAAGG TGTTCCAGGA CAGCAAGGCC TGGGAGAACT TCCGCACCCT CACCGACCTG
CTGCTGCGCT TCGAGCCCAA CCTGGATGTG GAGCAGGCCG AGGTCAACTT CGGCTGGAAC
CACCTGGAGC CCTATGCCCA TTTCCTGCTC TCTGTCTTCT TCGTCATCTT CTCCTTCCCC
ATCGCCAGCA AGGACTGCAT CCCCTGCTCG GAGCTGGCTG TCATCACCGG CTTCTTTACC
GTGACCAGCT ACCTGAGCCT GAGCACCCAT GCAGAGCCCT ACACGCGCAG GGCCCTGGCC
ACCGAGGTCA CCGCCGGCCT GCTATCGCTG CTGCCCTCCA TGCCCTTGAA TTGGCCCTAC
CTGAAGGTCC TTGGCCAGAC CTTCATCACC GTGCCTGTCG GCCACCTGGT CGTCCTCAAC
GTCAGCGTCC CGTGCCTGCT CTATGTCTAC CTGCTCTATC TCTTCTTCCG CATGGCACAG
CTGAGGAATT TCAAGGGCAC CTACTGCTAC CTTGTGCCCT ACCTGGTGTG CTTCATGTGG
TGTGAGCTCT CCGTGGTCAT CCTGCTGGAG TCCACCGGCC TGGGGCTGCT CCGCGCCTCC
ATCGGCTACT TCCTCTTCCT CTTTGCCCTC CCCATCCTGG TGGCCGGCCT GGCCCTGGTG
GGCGTGCTGC AGTTCGCCCG GTGGTTCACG TCTCTGGAGC TCACCAAGAT CGCAGTCACC
GTGGCGGTCT GTAGTGTGCC CCTGCTGTTG CGCTGGTGGA CCAAGGCCAG CTTCTCTGTG
GTGGGGATGG TGAAGTCCCT GACGCGGAGC TCCATGGTCA AGCTCATCCT GGTGTGGCTC
ACGGCCATCG TGCTGTTCTG CTGGTTCTAT GTGTACCGCT CAGAGGGCAT GAAGGTCTAC
AACTCCACAC TGACCTGGCA GCAGTATGGT GCGCTGTGCG GGCCACGCGC CTGGAAGGAG
ACCAACATGG CGCGCACCCA GATCCTCTGC AGCCACCTGG AGGGCCACAG GGTCACGTGG
ACCGGCCGCT TCAAGTACGT CCGCGTGACT GACATCGACA ACAGCGCCGA GTCTGCCATC
AACATGCTCC CGTTCTTCAT CGGCGACTGG ATGCGCTGCC TCTACGGCGA GGCCTACCCT
GCCTGCAGCC CTGGCAACAC CTCCACGGCC GAGGAGGAGC TCTGTCGCCT TAAGCTGCTG
GCCAAGCACC CCTGCCACAT CAAGAAGTTC GACCGCTACA AGTTTGAGAT TACCGTGGGC
ATGCCATTCA GCAGCGGCGC TGACGGCTCG CGCAGCCGCG AGGAGGACGA CGTCACCAAG
GACATCGTGC TGCGGGCCAG CAGCGAGTTC AAGAGCGTGC TGCTCAGCCT GCGCCAGGGC
AGCCTCATCG AGTTCAGCAC CATCCTGGAG GGCCGCCTGG GCAGCAAGTG GCCTGTCTTC
GAGCTCAAGG CCATCAGCTG CCTCAACTGC ATGGCCCAGC TCTCACCCAC CAGGCGGCAC
GTGAAGATCG AGCACGACTG GCGCAGCACC GTGCATGGCG CCGTGAAGTT CGCCTTCGAC
TTCTTTTTCT TCCCATTCCT GTCGGCGGCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Autosomal dominant diabetes arising from a Wolfram syndrome 1 mutation
Diabetes 62 (11), 3943-3950 (2013)
Bonnycastle LL, Chines PS, Hara T, Huyghe JR, Swift AJ, Heikinheimo P, Mahadevan J, Peltonen S, Huopio H, Nuutila P, Narisu N, Goldfeder RL, Stitzel ML, Lu S, Boehnke M, Urano F, Collins FS and Laakso M.


book

Molecular characterization of WFS1 in an Iranian family with Wolfram syndrome reveals a novel frameshift mutation associated with early symptoms
Gene 528 (2), 309-313 (2013)
Sobhani M, Tabatabaiefar MA, Rajab A, Kajbafzadeh AM and Noori-Daloii MR.


book

WFS1 variants in Finnish patients with diabetes mellitus, sensorineural hearing impairment or optic atrophy, and in suicide victims
J. Hum. Genet. 58 (8), 495-500 (2013)
Kytovuori L, Seppanen A, Martikainen MH, Moilanen JS, Kamppari S, Sarkioja T, Remes AM, Rasanen P, Ronnemaa T and Majamaa K.


book

Association of aggression with a novel microRNA binding site polymorphism in the wolframin gene
Am. J. Med. Genet. B Neuropsychiatr. Genet. 162B (4), 404-412 (2013)
Kovacs-Nagy R, Elek Z, Szekely A, Nanasi T, Sasvari-Szekely M and Ronai Z.


book

Diabetes insipidus, diabetes mellitus, optic atrophy and deafness (DIDMOAD) caused by mutations in a novel gene (wolframin) coding for a predicted transmembrane protein
Hum. Mol. Genet. 7 (13), 2021-2028 (1998)
Strom TM, Hortnagel K, Hofmann S, Gekeler F, Scharfe C, Rabl W, Gerbitz KD and Meitinger T.


book

A gene encoding a transmembrane protein is mutated in patients with diabetes mellitus and optic atrophy (Wolfram syndrome)
Nat. Genet. 20 (2), 143-148 (1998)
Inoue H, Tanizawa Y, Wasson J, Behn P, Kalidas K, Bernal-Mizrachi E, Mueckler M, Marshall H, Donis-Keller H, Crock P, Rogers D, Mikuni M, Kumashiro H, Higashi K, Sobue G, Oka Y and Permutt MA.


book

A gene for autosomal dominant nonsyndromic hereditary hearing impairment maps to 4p16.3
Hum. Mol. Genet. 4 (10), 1967-1972 (1995)
Lesperance MM, Hall JW 3rd, Bess FH, Fukushima K, Jain PK, Ploplis B, San Agustin TB, Skarka H, Smith RJ and Wills M.


book

Linkage of the gene for Wolfram syndrome to markers on the short arm of chromosome 4
Nat. Genet. 8 (1), 95-97 (1994)
Polymeropoulos MH, Swift RG and Swift M.


book

WFS1-Related Disorders
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Tranebjaerg,L., Barrett,T. and Rendtorff,N.D.


book

Deafness and Hereditary Hearing Loss Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Smith,R.J.H., Shearer,A.E., Hildebrand,M.S. and Van Camp,G.


 
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