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WT1 Wilms tumor 1 [Homo sapiens (human)]

Gene Symbol WT1
Entrez Gene ID 7490
Full Name Wilms tumor 1
Synonyms AWT1, EWS-WT1, GUD, NPHS4, WAGR, WIT-2, WT33
General protein information
Preferred Names
Wilms tumor protein
Names
Wilms tumor protein
amino-terminal domain of EWS|last three zinc fingers of the DNA-binding domain of WT1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p13

Summary This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]. lac of sum
Disorder MIM:

607102

Disorder Html: Wilms tumor, type 1, 194070 (3); Denys-Drash syndrome, 194080 (3);
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu27056 NM_000378 Homo sapiens Wilms tumor 1 (WT1), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK Not in stock -1 Starting from $99.00
OHu27316 NM_001198551 Homo sapiens Wilms tumor 1 (WT1), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu27419 NM_024424 Homo sapiens Wilms tumor 1 (WT1), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK Not in stock -1 Starting from $99.00
OHu27290 NM_001198552 Homo sapiens Wilms tumor 1 (WT1), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu27351 NM_024426 Homo sapiens Wilms tumor 1 (WT1), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK In stock -1 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu27056D
Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product Wilms tumor protein isoform A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK291736.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Oct 23, 2010 this sequence version replaced gi:65507713. Summary: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]. Transcript Variant: This variant (A) lacks an alternate in-frame exon and uses an alternate, in-frame splice site in the 3' coding region, compared to variant D. This results in a shorter protein (isoform A), compared to isoform D. It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M30393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG CCACAGCACA
GGGTACGAGA GCGATAACCA CACAACGCCC ATCCTCTGCG GAGCCCAATA CAGAATACAC
ACGCACGGTG TCTTCAGAGG CATTCAGGAT GTGCGACGTG TGCCTGGAGT AGCCCCGACT
CTTGTACGGT CGGCATCTGA GACCAGTGAG AAACGCCCCT TCATGTGTGC TTACCCAGGC
TGCAATAAGA GATATTTTAA GCTGTCCCAC TTACAGATGC ACAGCAGGAA GCACACTGGT
GAGAAACCAT ACCAGTGTGA CTTCAAGGAC TGTGAACGAA GGTTTTCTCG TTCAGACCAG
CTCAAAAGAC ACCAAAGGAG ACATACAGGT GTGAAACCAT TCCAGTGTAA AACTTGTCAG
CGAAAGTTCT CCCGGTCCGA CCACCTGAAG ACCCACACCA GGACTCATAC AGGTGAAAAG
CCCTTCAGCT GTCGGTGGCC AAGTTGTCAG AAAAAGTTTG CCCGGTCAGA TGAATTAGTC
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_000369.3
CDS191..1684
Misc Feature(1)395..1306(+)
Misc Feature(2)728..1657(+)
Misc Feature(3)1325..1384(+)
Misc Feature(4)1331..1384(+)
Misc Feature(5)1337..1555(+)
Misc Feature(6)1358..1441(+)
Misc Feature(7)1406..1474(+)
Misc Feature(8)1406..1471(+)
Misc Feature(9)1436..1438(+)
Misc Feature(10)1451..1525(+)
Misc Feature(11)1496..1558(+)
Misc Feature(12)1496..1558(+)
Misc Feature(13)1496..1558(+)
Misc Feature(14)1496..1558(+)
Misc Feature(15)1511..1645(+)
Misc Feature(16)1520..1522(+)
Misc Feature(17)1532..1615(+)
Misc Feature(18)1580..1648(+)
Misc Feature(19)1580..1648(+)
Misc Feature(20)395..397(+)
Misc Feature(21)1185..1185(+)
Exon (1)1..836
Gene:WT1
Gene Synonym:
Exon (2)837..959
Gene:WT1
Gene Synonym:
Exon (3)960..1062
Gene:WT1
Gene Synonym:
Exon (4)1063..1140
Gene:WT1
Gene Synonym:
Exon (5)1141..1237
Gene:WT1
Gene Synonym:
Exon (6)1238..1388
Gene:WT1
Gene Synonym:
Exon (7)1389..1478
Gene:WT1
Gene Synonym:
Exon (8)1479..1562
Gene:WT1
Gene Synonym:
Exon (9)1563..2967
Gene:WT1
Gene Synonym:
Translation
Position Chain Variation Link
14 14 a, c dbSNP:772399951
54 54 c, g dbSNP:541850548
101 101 c, t dbSNP:5030133
113 113 c, t dbSNP:572838503
114 114 a, c dbSNP:552828543
115 115 c, t dbSNP:539323571
117 117 a, c dbSNP:570325451
119 119 -, ccca dbSNP:542898204
202 202 a, g dbSNP:556835183
209 209 a, c dbSNP:764111950
256 256 c, t dbSNP:755007049
258 258 a, g dbSNP:751641518
277 277 a, g dbSNP:766527734
290 290 c, t dbSNP:763147828
292 292 a, g dbSNP:537150542
297 297 c, t dbSNP:773550149
298 298 c, g dbSNP:555140661
299 299 a, g dbSNP:762288656
326 326 -, g dbSNP:776155094
337 337 c, g dbSNP:776954184
356 356 a, c, g dbSNP:2234581
368 368 a, g dbSNP:374404615
376 376 c, g dbSNP:771769393
385 385 g, t dbSNP:745506215
388 388 g, t dbSNP:2234582
391 391 g, t dbSNP:5030135
398 398 a, g dbSNP:528076586
416 416 c, g dbSNP:777617460
425 425 c, t dbSNP:756055892
434 434 a, g dbSNP:752686863
445 445 c, g dbSNP:766441234
455 455 a, g dbSNP:758512604
461 461 -, ggc dbSNP:770519620
466 466 g, t dbSNP:750588084
473 473 c, t dbSNP:567200038
484 484 a, c dbSNP:547333427
491 491 a, c dbSNP:762044578
502 502 c, g dbSNP:777059515
520 520 c, t dbSNP:1799925
528 528 c, t dbSNP:761252308
550 550 c, t dbSNP:776209354
556 556 c, g dbSNP:771681406
561 561 c, t dbSNP:745435848
566 566 a, c, t dbSNP:564706633
569 569 a, g dbSNP:773831663
573 573 c, t dbSNP:770618081
576 576 a, c dbSNP:748974419
577 577 a, g dbSNP:777527675
579 579 c, t dbSNP:769642496
581 581 a, c dbSNP:748045691
587 587 c, t dbSNP:544477985
592 592 a, g dbSNP:758361767
595 595 -, gcc dbSNP:772893663
596 596 c, t dbSNP:750548251
597 597 -, gcc dbSNP:760304811
602 602 a, t dbSNP:778937284
604 604 c, g dbSNP:531076591
607 607 c, t dbSNP:754173303
615 615 a, g dbSNP:764552529
619 619 a, g dbSNP:761166030
646 646 c, t dbSNP:753238865
650 650 a, g dbSNP:768165877
655 655 a, g dbSNP:758900425
658 658 c, t dbSNP:773922379
659 659 c, t dbSNP:770403127
678 678 a, g dbSNP:762660918
685 685 c, t dbSNP:772890986
688 688 a, c dbSNP:536728682
697 697 c, t dbSNP:747958111
718 718 c, t dbSNP:369870529
721 721 a, c dbSNP:121907911
731 731 g, t dbSNP:768644520
741 741 c, g dbSNP:745879965
742 742 g, t dbSNP:141135091
744 744 c, t dbSNP:757349907
753 753 c, t dbSNP:572926487
759 759 c, g dbSNP:778194188
761 761 a, g dbSNP:756501972
762 762 a, g dbSNP:753112302
766 766 a, g dbSNP:767850217
767 767 a, g, t dbSNP:377072761
771 771 c, g dbSNP:765879407
772 772 a, g dbSNP:762418497
784 784 c, t dbSNP:2234583
785 785 a, g dbSNP:9332973
790 790 c, g dbSNP:769467940
802 802 c, t dbSNP:761530541
803 803 g, t dbSNP:776426005
808 808 c, t dbSNP:576677344
813 813 a, g dbSNP:746964440
814 814 a, c dbSNP:775333473
816 816 a, c dbSNP:771024882
820 820 a, c dbSNP:749405227
821 821 a, g dbSNP:778069787
828 828 a, g dbSNP:756414084
830 830 a, c dbSNP:748435329
833 833 a, c, g dbSNP:373935628
837 837 a, g dbSNP:369444006
839 839 c, t dbSNP:746367691
846 846 c, t dbSNP:779451877
847 847 c, g dbSNP:756695525
849 849 c, t dbSNP:576902446
852 852 a, c, t dbSNP:556804456
853 853 c, g dbSNP:752438619
860 860 a, g dbSNP:767419243
861 861 c, g dbSNP:759464627
862 862 c, g dbSNP:760001898
864 864 c, t dbSNP:777166391
866 866 c, t dbSNP:766425764
870 870 c, g dbSNP:761913397
871 871 c, t dbSNP:9332974
883 883 a, g dbSNP:768828897
885 885 c, t dbSNP:760776653
886 886 c, t dbSNP:183263057
889 889 g, t dbSNP:772409488
892 892 c, g dbSNP:746241159
898 898 a, g dbSNP:779321799
905 905 a, t dbSNP:771455202
915 915 a, t dbSNP:749860238
921 921 a, t dbSNP:777119782
935 935 a, c, t dbSNP:2234584
938 938 a, g dbSNP:142653301
939 939 a, t dbSNP:377573993
941 941 a, g dbSNP:751432306
960 960 a, g dbSNP:372225738
978 978 c, t dbSNP:765230888
981 981 c, g dbSNP:756078681
986 986 c, t dbSNP:752801263
991 991 a, c, g dbSNP:536750501
993 993 a, g dbSNP:774626752
994 994 c, t dbSNP:766642594
1000 1000 c, g dbSNP:200222400
1005 1005 c, t dbSNP:138073760
1007 1007 c, g dbSNP:773527284
1011 1011 c, t dbSNP:770366595
1012 1012 c, g dbSNP:747613465
1023 1023 c, t dbSNP:776258782
1024 1024 c, t dbSNP:145425799
1027 1027 c, g dbSNP:548704325
1029 1029 a, g dbSNP:779813097
1034 1034 g, t dbSNP:368452754
1068 1068 -, atttataccaaatgaca dbSNP:587776573
1070 1070 a, t dbSNP:780092599
1073 1073 c, t dbSNP:758624956
1075 1075 c, t dbSNP:190440703
1076 1076 c, t dbSNP:547775568
1082 1082 a, g dbSNP:762325377
1086 1086 c, t dbSNP:267602852
1100 1100 a, g dbSNP:754336808
1114 1114 c, g dbSNP:764701739
1116 1116 c, t dbSNP:760219458
1120 1120 c, t dbSNP:775085343
1131 1131 a, c dbSNP:372630828
1144 1144 c, t dbSNP:375114529
1146 1146 a, g dbSNP:371021920
1153 1153 a, g dbSNP:752102247
1157 1157 a, g dbSNP:547783372
1159 1159 a, g dbSNP:757962229
1160 1160 a, g dbSNP:121907908
1162 1162 c, t dbSNP:750018485
1163 1163 a, g dbSNP:267602851
1173 1173 c, t dbSNP:764802290
1176 1176 a, c dbSNP:761627098
1182 1182 a, t dbSNP:548544187
1183 1183 c, t dbSNP:527655625
1187 1187 c, t dbSNP:142059681
1191 1191 a, g dbSNP:760654956
1195 1195 c, g dbSNP:775630812
1196 1196 c, g dbSNP:771117558
1198 1198 a, g dbSNP:2234590
1200 1200 a, g dbSNP:773532792
1207 1207 a, g dbSNP:17855567
1216 1216 a, c dbSNP:587778756
1218 1218 a, g dbSNP:769934402
1218 1218 -, g dbSNP:587776574
1224 1224 a, g, t dbSNP:150194429
1228 1228 a, t dbSNP:544966826
1235 1235 a, c dbSNP:747502066
1241 1241 a, g dbSNP:747377024
1243 1243 a, g dbSNP:780497248
1245 1245 a, g dbSNP:772590420
1246 1246 a, g dbSNP:16754
1248 1248 a, c, g, t dbSNP:554416372
1255 1255 c, t dbSNP:151034312
1259 1259 a, g dbSNP:755936509
1264 1264 a, c, t dbSNP:147126640
1265 1265 c, t dbSNP:754938612
1270 1270 a, t dbSNP:371168589
1277 1277 c, t dbSNP:765218093
1278 1278 a, g dbSNP:147241955
1279 1279 g, t dbSNP:776651253
1281 1281 c, g dbSNP:142937387
1282 1282 a, g dbSNP:377446096
1285 1285 a, g dbSNP:141834493
1306 1306 c, t dbSNP:147939483
1307 1307 c, t dbSNP:267602850
1312 1312 c, t dbSNP:200539928
1322 1322 c, t dbSNP:746353651
1332 1332 a, g dbSNP:121907904
1334 1334 a, c dbSNP:774976881
1335 1335 a, c, g dbSNP:748864758
1377 1377 g, t dbSNP:373176048
1393 1393 a, g dbSNP:767850636
1402 1402 c, t dbSNP:759955361
1405 1405 c, g dbSNP:774650640
1409 1409 a, g dbSNP:771494968
1421 1421 g, t dbSNP:121907905
1427 1427 c, t dbSNP:121907906
1428 1428 a, g dbSNP:144788858
1430 1430 a, g dbSNP:77183751
1439 1439 c, t dbSNP:121907910
1440 1440 a, g dbSNP:121907901
1462 1462 c, g dbSNP:121907907
1465 1465 a, t dbSNP:772822466
1470 1470 a, g dbSNP:769499906
1472 1472 c, t dbSNP:28942089
1490 1490 c, t dbSNP:28941777
1495 1495 a, g dbSNP:546743811
1502 1502 a, g dbSNP:751932589
1503 1503 c, t dbSNP:766697865
1509 1509 a, t dbSNP:533043871
1511 1511 c, t dbSNP:121907909
1517 1517 c, t dbSNP:28941779
1523 1523 c, t dbSNP:121907900
1524 1524 c, g dbSNP:121907903
1528 1528 c, t dbSNP:374799820
1529 1529 a, g dbSNP:28941778
1530 1530 a, g dbSNP:121907902
1543 1543 c, t dbSNP:769371345
1558 1558 c, t dbSNP:761414130
1578 1578 a, g dbSNP:267602849
1584 1584 a, g dbSNP:139893274
1589 1589 c, t dbSNP:761360324
1592 1592 a, g dbSNP:776256676
1593 1593 c, g dbSNP:763551837
1602 1602 a, g dbSNP:760370132
1642 1642 c, g dbSNP:369940913
1659 1659 c, t dbSNP:775063171
1662 1662 c, t dbSNP:528613270
1666 1666 a, c dbSNP:745663070
1669 1669 c, t dbSNP:267602848
1674 1674 c, t dbSNP:774228907
1677 1677 a, c, t dbSNP:749266841
1678 1678 a, g dbSNP:202118284
1683 1683 a, g dbSNP:148856160
1685 1685 a, g dbSNP:747197306
1686 1686 a, g, t dbSNP:758798048
1688 1688 c, g dbSNP:750842875
1689 1689 c, t dbSNP:765743928
1694 1694 c, t dbSNP:757807247
1696 1696 c, t dbSNP:77462662
1697 1697 a, g dbSNP:763645824
1700 1700 g, t dbSNP:2234594
1704 1704 a, g, t dbSNP:767103760
1713 1713 c, t dbSNP:759275928
1714 1714 c, t dbSNP:774104210
1719 1719 a, c dbSNP:376972948
1726 1726 a, g dbSNP:749213891
1730 1730 a, g dbSNP:773040516
1765 1765 c, t dbSNP:563643335
1768 1768 c, g dbSNP:749749213
1772 1772 a, g dbSNP:5030315
1794 1794 c, t dbSNP:757474299
1822 1822 a, g dbSNP:111351882
1824 1824 c, t dbSNP:770485644
1843 1843 c, g dbSNP:746218880
1892 1892 c, t dbSNP:560907250
1908 1908 a, g dbSNP:5030316
1951 1951 c, g dbSNP:5030317
1964 1964 g, t dbSNP:181881769
2023 2023 a, g dbSNP:544612574
2029 2029 a, g dbSNP:5030318
2049 2049 a, t dbSNP:557682653
2062 2062 a, c dbSNP:537329306
2063 2063 c, t dbSNP:746694121
2074 2074 a, t dbSNP:568703873
2075 2075 a, c dbSNP:777105291
2104 2104 g, t dbSNP:772298084
2113 2113 g, t dbSNP:554888133
2137 2137 c, g dbSNP:761025226
2143 2143 a, g dbSNP:535181585
2149 2149 a, c dbSNP:189407871
2160 2160 a, g dbSNP:757906937
2197 2197 c, t dbSNP:575602262
2198 2198 a, g dbSNP:372622383
2205 2205 a, g dbSNP:765145800
2226 2226 a, g dbSNP:142726499
2249 2249 a, t dbSNP:754974695
2254 2254 -, t dbSNP:774867727
2257 2257 -, g dbSNP:79335260
2258 2258 -, g dbSNP:5030319
2286 2286 a, g dbSNP:5030320
2288 2288 a, c dbSNP:766220081
2291 2291 a, g dbSNP:550080671
2336 2336 a, t dbSNP:761903425
2350 2350 a, g dbSNP:374306749
2353 2353 a, t dbSNP:764209237
2387 2387 a, g dbSNP:763107748
2458 2458 a, c dbSNP:184847211
2476 2476 a, g dbSNP:746314166
2481 2481 a, g dbSNP:148478722
2504 2504 c, g dbSNP:1042347
2519 2519 a, g dbSNP:5030322
2548 2548 a, g, t dbSNP:5030323
2556 2556 c, t dbSNP:576023116
2581 2581 c, g dbSNP:5030324
2587 2587 a, g dbSNP:5030325
2597 2597 c, t dbSNP:760142967
2633 2633 a, c dbSNP:776814761
2639 2639 a, g dbSNP:771360373
2656 2656 -, a dbSNP:72519751
2662 2662 -, a dbSNP:5030326
2686 2686 a, c dbSNP:575035138
2704 2704 -, cgtg dbSNP:780961917
2705 2705 -, gtgt dbSNP:58549495
2727 2727 -, gtgtg dbSNP:770524174
2729 2729 -, gtgtg dbSNP:758276052
2729 2729 -, gtg dbSNP:766176703
2731 2731 -, gtg dbSNP:750046431
2733 2733 -, gtgtgt dbSNP:147708114
2733 2733 -, gtgt dbSNP:761038390
2733 2733 -, g dbSNP:568803808
2734 2734 -, tgtgtt dbSNP:754104430
2736 2736 -, tgtt dbSNP:767995702
2737 2737 -, gt dbSNP:200382866
2746 2746 (tg)19, 20, 21, 22, 23 dbSNP:3138550
2782 2782 c, t dbSNP:5030327
2783 2783 a, g dbSNP:746578571
2816 2816 a, t dbSNP:5030328
2825 2825 a, t dbSNP:566276801
2851 2851 g, t dbSNP:771770230
2857 2857 a, c dbSNP:754404198
2893 2893 a, g dbSNP:5030329
2896 2896 c, t dbSNP:747653324
2899 2899 a, g dbSNP:539117133
2923 2923 a, t dbSNP:534396876

Target ORF information:

RefSeq Version NM_000378
Organism Homo sapiens (human)
Definition Homo sapiens Wilms tumor 1 (WT1), transcript variant A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_000378

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG CCACAGCACA
GGGTACGAGA GCGATAACCA CACAACGCCC ATCCTCTGCG GAGCCCAATA CAGAATACAC
ACGCACGGTG TCTTCAGAGG CATTCAGGAT GTGCGACGTG TGCCTGGAGT AGCCCCGACT
CTTGTACGGT CGGCATCTGA GACCAGTGAG AAACGCCCCT TCATGTGTGC TTACCCAGGC
TGCAATAAGA GATATTTTAA GCTGTCCCAC TTACAGATGC ACAGCAGGAA GCACACTGGT
GAGAAACCAT ACCAGTGTGA CTTCAAGGAC TGTGAACGAA GGTTTTCTCG TTCAGACCAG
CTCAAAAGAC ACCAAAGGAG ACATACAGGT GTGAAACCAT TCCAGTGTAA AACTTGTCAG
CGAAAGTTCT CCCGGTCCGA CCACCTGAAG ACCCACACCA GGACTCATAC AGGTGAAAAG
CCCTTCAGCT GTCGGTGGCC AAGTTGTCAG AAAAAGTTTG CCCGGTCAGA TGAATTAGTC
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu27316D
Sequence Information ORF Nucleotide Sequence (Length: 909bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product Wilms tumor protein isoform E
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG718348.1, BC032861.2, AK093168.1, M30393.1 and BC046461.1. This sequence is a reference standard in the RefSeqGene project. Summary: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]. Transcript Variant: This variant (E) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant D. This variant also uses an alternate, in-frame splice site in the 3' coding region, compared to variant D. The encoded isoform (E) has a distinct N-terminus and is shorter than isoform D. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032861.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA
GCCACCTTAA AGGGAGTTGC TGCTGGGAGC TCCAGCTCAG TGAAATGGAC AGAAGGGCAG
AGCAACCACA GCACAGGGTA CGAGAGCGAT AACCACACAA CGCCCATCCT CTGCGGAGCC
CAATACAGAA TACACACGCA CGGTGTCTTC AGAGGCATTC AGGATGTGCG ACGTGTGCCT
GGAGTAGCCC CGACTCTTGT ACGGTCGGCA TCTGAGACCA GTGAGAAACG CCCCTTCATG
TGTGCTTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC
AGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGTGA ACGAAGGTTT
TCTCGTTCAG ACCAGCTCAA AAGACACCAA AGGAGACATA CAGGTGTGAA ACCATTCCAG
TGTAAAACTT GTCAGCGAAA GTTCTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT
CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG
GCGCTTTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001185480.1
CDS279..1187
Misc Feature(1)231..233(+)
Misc Feature(2)285..809(+)
Misc Feature(3)783..>980(+)
Misc Feature(4)828..887(+)
Misc Feature(5)834..887(+)
Misc Feature(6)840..1058(+)
Misc Feature(7)861..944(+)
Misc Feature(8)909..977(+)
Misc Feature(9)909..974(+)
Misc Feature(10)954..1028(+)
Misc Feature(11)987..>1163(+)
Misc Feature(12)999..1061(+)
Misc Feature(13)999..1061(+)
Misc Feature(14)999..1061(+)
Misc Feature(15)999..1061(+)
Misc Feature(16)1014..1148(+)
Misc Feature(17)1035..1118(+)
Misc Feature(18)1083..1151(+)
Misc Feature(19)1083..1151(+)
Misc Feature(20)688..688(+)
Exon (1)1..288
Gene:WT1
Gene Synonym:
Exon (2)289..411
Gene:WT1
Gene Synonym:
Exon (3)412..514
Gene:WT1
Gene Synonym:
Exon (4)515..592
Gene:WT1
Gene Synonym:
Exon (5)593..643
Gene:WT1
Gene Synonym:
Exon (6)644..740
Gene:WT1
Gene Synonym:
Exon (7)741..891
Gene:WT1
Gene Synonym:
Exon (8)892..981
Gene:WT1
Gene Synonym:
Exon (9)982..1065
Gene:WT1
Gene Synonym:
Exon (10)1066..2470
Gene:WT1
Gene Synonym:
Translation
Position Chain Variation Link
4 4 c, g dbSNP:762096406
25 25 c, g dbSNP:565551027
26 26 a, g dbSNP:75870920
78 78 c, t dbSNP:557323865
84 84 c, g dbSNP:148234826
122 122 a, g dbSNP:577579117
123 123 -, g dbSNP:76500597
153 153 a, g dbSNP:536745067
200 200 g, t dbSNP:574176186
202 202 c, t dbSNP:766680590
219 219 a, g dbSNP:554252347
234 234 g, t dbSNP:760438749
239 239 a, g dbSNP:775453593
240 240 c, t dbSNP:771938327
242 242 a, c dbSNP:534172168
248 248 c, t dbSNP:773408487
257 257 c, t dbSNP:770062804
260 260 c, t dbSNP:748347306
268 268 c, t dbSNP:781316935
271 271 -, gc dbSNP:71873106
289 289 a, g dbSNP:369444006
291 291 c, t dbSNP:746367691
298 298 c, t dbSNP:779451877
299 299 c, g dbSNP:756695525
301 301 c, t dbSNP:576902446
304 304 a, c, t dbSNP:556804456
305 305 c, g dbSNP:752438619
312 312 a, g dbSNP:767419243
313 313 c, g dbSNP:759464627
314 314 c, g dbSNP:760001898
316 316 c, t dbSNP:777166391
318 318 c, t dbSNP:766425764
322 322 c, g dbSNP:761913397
323 323 c, t dbSNP:9332974
335 335 a, g dbSNP:768828897
337 337 c, t dbSNP:760776653
338 338 c, t dbSNP:183263057
341 341 g, t dbSNP:772409488
344 344 c, g dbSNP:746241159
350 350 a, g dbSNP:779321799
357 357 a, t dbSNP:771455202
367 367 a, t dbSNP:749860238
373 373 a, t dbSNP:777119782
387 387 a, c, t dbSNP:2234584
390 390 a, g dbSNP:142653301
391 391 a, t dbSNP:377573993
393 393 a, g dbSNP:751432306
412 412 a, g dbSNP:372225738
430 430 c, t dbSNP:765230888
433 433 c, g dbSNP:756078681
438 438 c, t dbSNP:752801263
443 443 a, c, g dbSNP:536750501
445 445 a, g dbSNP:774626752
446 446 c, t dbSNP:766642594
452 452 c, g dbSNP:200222400
457 457 c, t dbSNP:138073760
459 459 c, g dbSNP:773527284
463 463 c, t dbSNP:770366595
464 464 c, g dbSNP:747613465
475 475 c, t dbSNP:776258782
476 476 c, t dbSNP:145425799
479 479 c, g dbSNP:548704325
481 481 a, g dbSNP:779813097
486 486 g, t dbSNP:368452754
520 520 -, atttataccaaatgaca dbSNP:587776573
522 522 a, t dbSNP:780092599
525 525 c, t dbSNP:758624956
527 527 c, t dbSNP:190440703
528 528 c, t dbSNP:547775568
534 534 a, g dbSNP:762325377
538 538 c, t dbSNP:267602852
552 552 a, g dbSNP:754336808
566 566 c, g dbSNP:764701739
568 568 c, t dbSNP:760219458
572 572 c, t dbSNP:775085343
583 583 a, c dbSNP:372630828
593 593 a, c dbSNP:776902132
601 601 -, ctg dbSNP:769982733
604 604 c, g dbSNP:766054482
608 608 c, t dbSNP:371218185
616 616 c, t dbSNP:762688982
623 623 a, g dbSNP:758410591
637 637 a, g dbSNP:769618028
640 640 a, g dbSNP:748112905
647 647 c, t dbSNP:375114529
649 649 a, g dbSNP:371021920
656 656 a, g dbSNP:752102247
660 660 a, g dbSNP:547783372
662 662 a, g dbSNP:757962229
663 663 a, g dbSNP:121907908
665 665 c, t dbSNP:750018485
666 666 a, g dbSNP:267602851
676 676 c, t dbSNP:764802290
679 679 a, c dbSNP:761627098
685 685 a, t dbSNP:548544187
686 686 c, t dbSNP:527655625
690 690 c, t dbSNP:142059681
694 694 a, g dbSNP:760654956
698 698 c, g dbSNP:775630812
699 699 c, g dbSNP:771117558
701 701 a, g dbSNP:2234590
703 703 a, g dbSNP:773532792
710 710 a, g dbSNP:17855567
719 719 a, c dbSNP:587778756
721 721 a, g dbSNP:769934402
721 721 -, g dbSNP:587776574
727 727 a, g, t dbSNP:150194429
731 731 a, t dbSNP:544966826
738 738 a, c dbSNP:747502066
744 744 a, g dbSNP:747377024
746 746 a, g dbSNP:780497248
748 748 a, g dbSNP:772590420
749 749 a, g dbSNP:16754
751 751 a, c, g, t dbSNP:554416372
758 758 c, t dbSNP:151034312
762 762 a, g dbSNP:755936509
767 767 a, c, t dbSNP:147126640
768 768 c, t dbSNP:754938612
773 773 a, t dbSNP:371168589
780 780 c, t dbSNP:765218093
781 781 a, g dbSNP:147241955
782 782 g, t dbSNP:776651253
784 784 c, g dbSNP:142937387
785 785 a, g dbSNP:377446096
788 788 a, g dbSNP:141834493
809 809 c, t dbSNP:147939483
810 810 c, t dbSNP:267602850
815 815 c, t dbSNP:200539928
825 825 c, t dbSNP:746353651
835 835 a, g dbSNP:121907904
837 837 a, c dbSNP:774976881
838 838 a, c, g dbSNP:748864758
880 880 g, t dbSNP:373176048
896 896 a, g dbSNP:767850636
905 905 c, t dbSNP:759955361
908 908 c, g dbSNP:774650640
912 912 a, g dbSNP:771494968
924 924 g, t dbSNP:121907905
930 930 c, t dbSNP:121907906
931 931 a, g dbSNP:144788858
933 933 a, g dbSNP:77183751
942 942 c, t dbSNP:121907910
943 943 a, g dbSNP:121907901
965 965 c, g dbSNP:121907907
968 968 a, t dbSNP:772822466
973 973 a, g dbSNP:769499906
975 975 c, t dbSNP:28942089
993 993 c, t dbSNP:28941777
998 998 a, g dbSNP:546743811
1005 1005 a, g dbSNP:751932589
1006 1006 c, t dbSNP:766697865
1012 1012 a, t dbSNP:533043871
1014 1014 c, t dbSNP:121907909
1020 1020 c, t dbSNP:28941779
1026 1026 c, t dbSNP:121907900
1027 1027 c, g dbSNP:121907903
1031 1031 c, t dbSNP:374799820
1032 1032 a, g dbSNP:28941778
1033 1033 a, g dbSNP:121907902
1046 1046 c, t dbSNP:769371345
1061 1061 c, t dbSNP:761414130
1081 1081 a, g dbSNP:267602849
1087 1087 a, g dbSNP:139893274
1092 1092 c, t dbSNP:761360324
1095 1095 a, g dbSNP:776256676
1096 1096 c, g dbSNP:763551837
1105 1105 a, g dbSNP:760370132
1145 1145 c, g dbSNP:369940913
1162 1162 c, t dbSNP:775063171
1165 1165 c, t dbSNP:528613270
1169 1169 a, c dbSNP:745663070
1172 1172 c, t dbSNP:267602848
1177 1177 c, t dbSNP:774228907
1180 1180 a, c, t dbSNP:749266841
1181 1181 a, g dbSNP:202118284
1186 1186 a, g dbSNP:148856160
1188 1188 a, g dbSNP:747197306
1189 1189 a, g, t dbSNP:758798048
1191 1191 c, g dbSNP:750842875
1192 1192 c, t dbSNP:765743928
1197 1197 c, t dbSNP:757807247
1199 1199 c, t dbSNP:77462662
1200 1200 a, g dbSNP:763645824
1203 1203 g, t dbSNP:2234594
1207 1207 a, g, t dbSNP:767103760
1216 1216 c, t dbSNP:759275928
1217 1217 c, t dbSNP:774104210
1222 1222 a, c dbSNP:376972948
1229 1229 a, g dbSNP:749213891
1233 1233 a, g dbSNP:773040516
1268 1268 c, t dbSNP:563643335
1271 1271 c, g dbSNP:749749213
1275 1275 a, g dbSNP:5030315
1297 1297 c, t dbSNP:757474299
1325 1325 a, g dbSNP:111351882
1327 1327 c, t dbSNP:770485644
1346 1346 c, g dbSNP:746218880
1395 1395 c, t dbSNP:560907250
1411 1411 a, g dbSNP:5030316
1454 1454 c, g dbSNP:5030317
1467 1467 g, t dbSNP:181881769
1526 1526 a, g dbSNP:544612574
1532 1532 a, g dbSNP:5030318
1552 1552 a, t dbSNP:557682653
1565 1565 a, c dbSNP:537329306
1566 1566 c, t dbSNP:746694121
1577 1577 a, t dbSNP:568703873
1578 1578 a, c dbSNP:777105291
1607 1607 g, t dbSNP:772298084
1616 1616 g, t dbSNP:554888133
1640 1640 c, g dbSNP:761025226
1646 1646 a, g dbSNP:535181585
1652 1652 a, c dbSNP:189407871
1663 1663 a, g dbSNP:757906937
1700 1700 c, t dbSNP:575602262
1701 1701 a, g dbSNP:372622383
1708 1708 a, g dbSNP:765145800
1729 1729 a, g dbSNP:142726499
1752 1752 a, t dbSNP:754974695
1757 1757 -, t dbSNP:774867727
1760 1760 -, g dbSNP:79335260
1761 1761 -, g dbSNP:5030319
1789 1789 a, g dbSNP:5030320
1791 1791 a, c dbSNP:766220081
1794 1794 a, g dbSNP:550080671
1839 1839 a, t dbSNP:761903425
1853 1853 a, g dbSNP:374306749
1856 1856 a, t dbSNP:764209237
1890 1890 a, g dbSNP:763107748
1961 1961 a, c dbSNP:184847211
1979 1979 a, g dbSNP:746314166
1984 1984 a, g dbSNP:148478722
2007 2007 c, g dbSNP:1042347
2022 2022 a, g dbSNP:5030322
2051 2051 a, g, t dbSNP:5030323
2059 2059 c, t dbSNP:576023116
2084 2084 c, g dbSNP:5030324
2090 2090 a, g dbSNP:5030325
2100 2100 c, t dbSNP:760142967
2136 2136 a, c dbSNP:776814761
2142 2142 a, g dbSNP:771360373
2159 2159 -, a dbSNP:72519751
2165 2165 -, a dbSNP:5030326
2189 2189 a, c dbSNP:575035138
2207 2207 -, cgtg dbSNP:780961917
2208 2208 -, gtgt dbSNP:58549495
2230 2230 -, gtgtg dbSNP:770524174
2232 2232 -, gtgtg dbSNP:758276052
2232 2232 -, gtg dbSNP:766176703
2234 2234 -, gtg dbSNP:750046431
2236 2236 -, gtgtgt dbSNP:147708114
2236 2236 -, gtgt dbSNP:761038390
2236 2236 -, g dbSNP:568803808
2237 2237 -, tgtgtt dbSNP:754104430
2239 2239 -, tgtt dbSNP:767995702
2240 2240 -, gt dbSNP:200382866
2249 2249 (tg)19, 20, 21, 22, 23 dbSNP:3138550
2285 2285 c, t dbSNP:5030327
2286 2286 a, g dbSNP:746578571
2319 2319 a, t dbSNP:5030328
2328 2328 a, t dbSNP:566276801
2354 2354 g, t dbSNP:771770230
2360 2360 a, c dbSNP:754404198
2396 2396 a, g dbSNP:5030329
2399 2399 c, t dbSNP:747653324
2402 2402 a, g dbSNP:539117133
2426 2426 a, t dbSNP:534396876

Target ORF information:

RefSeq Version NM_001198551
Organism Homo sapiens (human)
Definition Homo sapiens Wilms tumor 1 (WT1), transcript variant E, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001198551

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA
GCCACCTTAA AGGGAGTTGC TGCTGGGAGC TCCAGCTCAG TGAAATGGAC AGAAGGGCAG
AGCAACCACA GCACAGGGTA CGAGAGCGAT AACCACACAA CGCCCATCCT CTGCGGAGCC
CAATACAGAA TACACACGCA CGGTGTCTTC AGAGGCATTC AGGATGTGCG ACGTGTGCCT
GGAGTAGCCC CGACTCTTGT ACGGTCGGCA TCTGAGACCA GTGAGAAACG CCCCTTCATG
TGTGCTTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC
AGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGTGA ACGAAGGTTT
TCTCGTTCAG ACCAGCTCAA AAGACACCAA AGGAGACATA CAGGTGTGAA ACCATTCCAG
TGTAAAACTT GTCAGCGAAA GTTCTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT
CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu27419D
Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product Wilms tumor protein isoform B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK291736.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Oct 23, 2010 this sequence version replaced gi:65507816. Summary: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]. Transcript Variant: This variant (B) uses an alternate, in-frame splice site in the 3' coding region, compared to variant D. This results in a shorter protein (isoform B), compared to isoform D. It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291736.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG AGTTGCTGCT
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACAGCAC AGGGTACGAG
AGCGATAACC ACACAACGCC CATCCTCTGC GGAGCCCAAT ACAGAATACA CACGCACGGT
GTCTTCAGAG GCATTCAGGA TGTGCGACGT GTGCCTGGAG TAGCCCCGAC TCTTGTACGG
TCGGCATCTG AGACCAGTGA GAAACGCCCC TTCATGTGTG CTTACCCAGG CTGCAATAAG
AGATATTTTA AGCTGTCCCA CTTACAGATG CACAGCAGGA AGCACACTGG TGAGAAACCA
TACCAGTGTG ACTTCAAGGA CTGTGAACGA AGGTTTTCTC GTTCAGACCA GCTCAAAAGA
CACCAAAGGA GACATACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTC
TCCCGGTCCG ACCACCTGAA GACCCACACC AGGACTCATA CAGGTGAAAA GCCCTTCAGC
TGTCGGTGGC CAAGTTGTCA GAAAAAGTTT GCCCGGTCAG ATGAATTAGT CCGCCATCAC
AACATGCATC AGAGAAACAT GACCAAACTC CAGCTGGCGC TTTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_077742.2
CDS191..1735
Misc Feature(1)395..1357(+)
Misc Feature(2)1346..1603(+)
Misc Feature(3)1376..1435(+)
Misc Feature(4)1382..1435(+)
Misc Feature(5)1388..1606(+)
Misc Feature(6)1409..1492(+)
Misc Feature(7)1457..1525(+)
Misc Feature(8)1457..1522(+)
Misc Feature(9)1487..1489(+)
Misc Feature(10)1502..1576(+)
Misc Feature(11)1535..>1711(+)
Misc Feature(12)1547..1609(+)
Misc Feature(13)1547..1609(+)
Misc Feature(14)1547..1609(+)
Misc Feature(15)1547..1609(+)
Misc Feature(16)1562..1696(+)
Misc Feature(17)1571..1573(+)
Misc Feature(18)1583..1666(+)
Misc Feature(19)1631..1699(+)
Misc Feature(20)1631..1699(+)
Misc Feature(21)395..397(+)
Misc Feature(22)1236..1236(+)
Exon (1)1..836
Gene:WT1
Gene Synonym:
Exon (2)837..959
Gene:WT1
Gene Synonym:
Exon (3)960..1062
Gene:WT1
Gene Synonym:
Exon (4)1063..1140
Gene:WT1
Gene Synonym:
Exon (5)1141..1191
Gene:WT1
Gene Synonym:
Exon (6)1192..1288
Gene:WT1
Gene Synonym:
Exon (7)1289..1439
Gene:WT1
Gene Synonym:
Exon (8)1440..1529
Gene:WT1
Gene Synonym:
Exon (9)1530..1613
Gene:WT1
Gene Synonym:
Exon (10)1614..3018
Gene:WT1
Gene Synonym:
Translation
Position Chain Variation Link
14 14 a, c dbSNP:772399951
54 54 c, g dbSNP:541850548
101 101 c, t dbSNP:5030133
113 113 c, t dbSNP:572838503
114 114 a, c dbSNP:552828543
115 115 c, t dbSNP:539323571
117 117 a, c dbSNP:570325451
119 119 -, ccca dbSNP:542898204
202 202 a, g dbSNP:556835183
209 209 a, c dbSNP:764111950
256 256 c, t dbSNP:755007049
258 258 a, g dbSNP:751641518
277 277 a, g dbSNP:766527734
290 290 c, t dbSNP:763147828
292 292 a, g dbSNP:537150542
297 297 c, t dbSNP:773550149
298 298 c, g dbSNP:555140661
299 299 a, g dbSNP:762288656
326 326 -, g dbSNP:776155094
337 337 c, g dbSNP:776954184
356 356 a, c, g dbSNP:2234581
368 368 a, g dbSNP:374404615
376 376 c, g dbSNP:771769393
385 385 g, t dbSNP:745506215
388 388 g, t dbSNP:2234582
391 391 g, t dbSNP:5030135
398 398 a, g dbSNP:528076586
416 416 c, g dbSNP:777617460
425 425 c, t dbSNP:756055892
434 434 a, g dbSNP:752686863
445 445 c, g dbSNP:766441234
455 455 a, g dbSNP:758512604
461 461 -, ggc dbSNP:770519620
466 466 g, t dbSNP:750588084
473 473 c, t dbSNP:567200038
484 484 a, c dbSNP:547333427
491 491 a, c dbSNP:762044578
502 502 c, g dbSNP:777059515
520 520 c, t dbSNP:1799925
528 528 c, t dbSNP:761252308
550 550 c, t dbSNP:776209354
556 556 c, g dbSNP:771681406
561 561 c, t dbSNP:745435848
566 566 a, c, t dbSNP:564706633
569 569 a, g dbSNP:773831663
573 573 c, t dbSNP:770618081
576 576 a, c dbSNP:748974419
577 577 a, g dbSNP:777527675
579 579 c, t dbSNP:769642496
581 581 a, c dbSNP:748045691
587 587 c, t dbSNP:544477985
592 592 a, g dbSNP:758361767
595 595 -, gcc dbSNP:772893663
596 596 c, t dbSNP:750548251
597 597 -, gcc dbSNP:760304811
602 602 a, t dbSNP:778937284
604 604 c, g dbSNP:531076591
607 607 c, t dbSNP:754173303
615 615 a, g dbSNP:764552529
619 619 a, g dbSNP:761166030
646 646 c, t dbSNP:753238865
650 650 a, g dbSNP:768165877
655 655 a, g dbSNP:758900425
658 658 c, t dbSNP:773922379
659 659 c, t dbSNP:770403127
678 678 a, g dbSNP:762660918
685 685 c, t dbSNP:772890986
688 688 a, c dbSNP:536728682
697 697 c, t dbSNP:747958111
718 718 c, t dbSNP:369870529
721 721 a, c dbSNP:121907911
731 731 g, t dbSNP:768644520
741 741 c, g dbSNP:745879965
742 742 g, t dbSNP:141135091
744 744 c, t dbSNP:757349907
753 753 c, t dbSNP:572926487
759 759 c, g dbSNP:778194188
761 761 a, g dbSNP:756501972
762 762 a, g dbSNP:753112302
766 766 a, g dbSNP:767850217
767 767 a, g, t dbSNP:377072761
771 771 c, g dbSNP:765879407
772 772 a, g dbSNP:762418497
784 784 c, t dbSNP:2234583
785 785 a, g dbSNP:9332973
790 790 c, g dbSNP:769467940
802 802 c, t dbSNP:761530541
803 803 g, t dbSNP:776426005
808 808 c, t dbSNP:576677344
813 813 a, g dbSNP:746964440
814 814 a, c dbSNP:775333473
816 816 a, c dbSNP:771024882
820 820 a, c dbSNP:749405227
821 821 a, g dbSNP:778069787
828 828 a, g dbSNP:756414084
830 830 a, c dbSNP:748435329
833 833 a, c, g dbSNP:373935628
837 837 a, g dbSNP:369444006
839 839 c, t dbSNP:746367691
846 846 c, t dbSNP:779451877
847 847 c, g dbSNP:756695525
849 849 c, t dbSNP:576902446
852 852 a, c, t dbSNP:556804456
853 853 c, g dbSNP:752438619
860 860 a, g dbSNP:767419243
861 861 c, g dbSNP:759464627
862 862 c, g dbSNP:760001898
864 864 c, t dbSNP:777166391
866 866 c, t dbSNP:766425764
870 870 c, g dbSNP:761913397
871 871 c, t dbSNP:9332974
883 883 a, g dbSNP:768828897
885 885 c, t dbSNP:760776653
886 886 c, t dbSNP:183263057
889 889 g, t dbSNP:772409488
892 892 c, g dbSNP:746241159
898 898 a, g dbSNP:779321799
905 905 a, t dbSNP:771455202
915 915 a, t dbSNP:749860238
921 921 a, t dbSNP:777119782
935 935 a, c, t dbSNP:2234584
938 938 a, g dbSNP:142653301
939 939 a, t dbSNP:377573993
941 941 a, g dbSNP:751432306
960 960 a, g dbSNP:372225738
978 978 c, t dbSNP:765230888
981 981 c, g dbSNP:756078681
986 986 c, t dbSNP:752801263
991 991 a, c, g dbSNP:536750501
993 993 a, g dbSNP:774626752
994 994 c, t dbSNP:766642594
1000 1000 c, g dbSNP:200222400
1005 1005 c, t dbSNP:138073760
1007 1007 c, g dbSNP:773527284
1011 1011 c, t dbSNP:770366595
1012 1012 c, g dbSNP:747613465
1023 1023 c, t dbSNP:776258782
1024 1024 c, t dbSNP:145425799
1027 1027 c, g dbSNP:548704325
1029 1029 a, g dbSNP:779813097
1034 1034 g, t dbSNP:368452754
1068 1068 -, atttataccaaatgaca dbSNP:587776573
1070 1070 a, t dbSNP:780092599
1073 1073 c, t dbSNP:758624956
1075 1075 c, t dbSNP:190440703
1076 1076 c, t dbSNP:547775568
1082 1082 a, g dbSNP:762325377
1086 1086 c, t dbSNP:267602852
1100 1100 a, g dbSNP:754336808
1114 1114 c, g dbSNP:764701739
1116 1116 c, t dbSNP:760219458
1120 1120 c, t dbSNP:775085343
1131 1131 a, c dbSNP:372630828
1141 1141 a, c dbSNP:776902132
1149 1149 -, ctg dbSNP:769982733
1152 1152 c, g dbSNP:766054482
1156 1156 c, t dbSNP:371218185
1164 1164 c, t dbSNP:762688982
1171 1171 a, g dbSNP:758410591
1185 1185 a, g dbSNP:769618028
1188 1188 a, g dbSNP:748112905
1195 1195 c, t dbSNP:375114529
1197 1197 a, g dbSNP:371021920
1204 1204 a, g dbSNP:752102247
1208 1208 a, g dbSNP:547783372
1210 1210 a, g dbSNP:757962229
1211 1211 a, g dbSNP:121907908
1213 1213 c, t dbSNP:750018485
1214 1214 a, g dbSNP:267602851
1224 1224 c, t dbSNP:764802290
1227 1227 a, c dbSNP:761627098
1233 1233 a, t dbSNP:548544187
1234 1234 c, t dbSNP:527655625
1238 1238 c, t dbSNP:142059681
1242 1242 a, g dbSNP:760654956
1246 1246 c, g dbSNP:775630812
1247 1247 c, g dbSNP:771117558
1249 1249 a, g dbSNP:2234590
1251 1251 a, g dbSNP:773532792
1258 1258 a, g dbSNP:17855567
1267 1267 a, c dbSNP:587778756
1269 1269 a, g dbSNP:769934402
1269 1269 -, g dbSNP:587776574
1275 1275 a, g, t dbSNP:150194429
1279 1279 a, t dbSNP:544966826
1286 1286 a, c dbSNP:747502066
1292 1292 a, g dbSNP:747377024
1294 1294 a, g dbSNP:780497248
1296 1296 a, g dbSNP:772590420
1297 1297 a, g dbSNP:16754
1299 1299 a, c, g, t dbSNP:554416372
1306 1306 c, t dbSNP:151034312
1310 1310 a, g dbSNP:755936509
1315 1315 a, c, t dbSNP:147126640
1316 1316 c, t dbSNP:754938612
1321 1321 a, t dbSNP:371168589
1328 1328 c, t dbSNP:765218093
1329 1329 a, g dbSNP:147241955
1330 1330 g, t dbSNP:776651253
1332 1332 c, g dbSNP:142937387
1333 1333 a, g dbSNP:377446096
1336 1336 a, g dbSNP:141834493
1357 1357 c, t dbSNP:147939483
1358 1358 c, t dbSNP:267602850
1363 1363 c, t dbSNP:200539928
1373 1373 c, t dbSNP:746353651
1383 1383 a, g dbSNP:121907904
1385 1385 a, c dbSNP:774976881
1386 1386 a, c, g dbSNP:748864758
1428 1428 g, t dbSNP:373176048
1444 1444 a, g dbSNP:767850636
1453 1453 c, t dbSNP:759955361
1456 1456 c, g dbSNP:774650640
1460 1460 a, g dbSNP:771494968
1472 1472 g, t dbSNP:121907905
1478 1478 c, t dbSNP:121907906
1479 1479 a, g dbSNP:144788858
1481 1481 a, g dbSNP:77183751
1490 1490 c, t dbSNP:121907910
1491 1491 a, g dbSNP:121907901
1513 1513 c, g dbSNP:121907907
1516 1516 a, t dbSNP:772822466
1521 1521 a, g dbSNP:769499906
1523 1523 c, t dbSNP:28942089
1541 1541 c, t dbSNP:28941777
1546 1546 a, g dbSNP:546743811
1553 1553 a, g dbSNP:751932589
1554 1554 c, t dbSNP:766697865
1560 1560 a, t dbSNP:533043871
1562 1562 c, t dbSNP:121907909
1568 1568 c, t dbSNP:28941779
1574 1574 c, t dbSNP:121907900
1575 1575 c, g dbSNP:121907903
1579 1579 c, t dbSNP:374799820
1580 1580 a, g dbSNP:28941778
1581 1581 a, g dbSNP:121907902
1594 1594 c, t dbSNP:769371345
1609 1609 c, t dbSNP:761414130
1629 1629 a, g dbSNP:267602849
1635 1635 a, g dbSNP:139893274
1640 1640 c, t dbSNP:761360324
1643 1643 a, g dbSNP:776256676
1644 1644 c, g dbSNP:763551837
1653 1653 a, g dbSNP:760370132
1693 1693 c, g dbSNP:369940913
1710 1710 c, t dbSNP:775063171
1713 1713 c, t dbSNP:528613270
1717 1717 a, c dbSNP:745663070
1720 1720 c, t dbSNP:267602848
1725 1725 c, t dbSNP:774228907
1728 1728 a, c, t dbSNP:749266841
1729 1729 a, g dbSNP:202118284
1734 1734 a, g dbSNP:148856160
1736 1736 a, g dbSNP:747197306
1737 1737 a, g, t dbSNP:758798048
1739 1739 c, g dbSNP:750842875
1740 1740 c, t dbSNP:765743928
1745 1745 c, t dbSNP:757807247
1747 1747 c, t dbSNP:77462662
1748 1748 a, g dbSNP:763645824
1751 1751 g, t dbSNP:2234594
1755 1755 a, g, t dbSNP:767103760
1764 1764 c, t dbSNP:759275928
1765 1765 c, t dbSNP:774104210
1770 1770 a, c dbSNP:376972948
1777 1777 a, g dbSNP:749213891
1781 1781 a, g dbSNP:773040516
1816 1816 c, t dbSNP:563643335
1819 1819 c, g dbSNP:749749213
1823 1823 a, g dbSNP:5030315
1845 1845 c, t dbSNP:757474299
1873 1873 a, g dbSNP:111351882
1875 1875 c, t dbSNP:770485644
1894 1894 c, g dbSNP:746218880
1943 1943 c, t dbSNP:560907250
1959 1959 a, g dbSNP:5030316
2002 2002 c, g dbSNP:5030317
2015 2015 g, t dbSNP:181881769
2074 2074 a, g dbSNP:544612574
2080 2080 a, g dbSNP:5030318
2100 2100 a, t dbSNP:557682653
2113 2113 a, c dbSNP:537329306
2114 2114 c, t dbSNP:746694121
2125 2125 a, t dbSNP:568703873
2126 2126 a, c dbSNP:777105291
2155 2155 g, t dbSNP:772298084
2164 2164 g, t dbSNP:554888133
2188 2188 c, g dbSNP:761025226
2194 2194 a, g dbSNP:535181585
2200 2200 a, c dbSNP:189407871
2211 2211 a, g dbSNP:757906937
2248 2248 c, t dbSNP:575602262
2249 2249 a, g dbSNP:372622383
2256 2256 a, g dbSNP:765145800
2277 2277 a, g dbSNP:142726499
2300 2300 a, t dbSNP:754974695
2305 2305 -, t dbSNP:774867727
2308 2308 -, g dbSNP:79335260
2309 2309 -, g dbSNP:5030319
2337 2337 a, g dbSNP:5030320
2339 2339 a, c dbSNP:766220081
2342 2342 a, g dbSNP:550080671
2387 2387 a, t dbSNP:761903425
2401 2401 a, g dbSNP:374306749
2404 2404 a, t dbSNP:764209237
2438 2438 a, g dbSNP:763107748
2509 2509 a, c dbSNP:184847211
2527 2527 a, g dbSNP:746314166
2532 2532 a, g dbSNP:148478722
2555 2555 c, g dbSNP:1042347
2570 2570 a, g dbSNP:5030322
2599 2599 a, g, t dbSNP:5030323
2607 2607 c, t dbSNP:576023116
2632 2632 c, g dbSNP:5030324
2638 2638 a, g dbSNP:5030325
2648 2648 c, t dbSNP:760142967
2684 2684 a, c dbSNP:776814761
2690 2690 a, g dbSNP:771360373
2707 2707 -, a dbSNP:72519751
2713 2713 -, a dbSNP:5030326
2737 2737 a, c dbSNP:575035138
2755 2755 -, cgtg dbSNP:780961917
2756 2756 -, gtgt dbSNP:58549495
2778 2778 -, gtgtg dbSNP:770524174
2780 2780 -, gtgtg dbSNP:758276052
2780 2780 -, gtg dbSNP:766176703
2782 2782 -, gtg dbSNP:750046431
2784 2784 -, gtgtgt dbSNP:147708114
2784 2784 -, gtgt dbSNP:761038390
2784 2784 -, g dbSNP:568803808
2785 2785 -, tgtgtt dbSNP:754104430
2787 2787 -, tgtt dbSNP:767995702
2788 2788 -, gt dbSNP:200382866
2797 2797 (tg)19, 20, 21, 22, 23 dbSNP:3138550
2833 2833 c, t dbSNP:5030327
2834 2834 a, g dbSNP:746578571
2867 2867 a, t dbSNP:5030328
2876 2876 a, t dbSNP:566276801
2902 2902 g, t dbSNP:771770230
2908 2908 a, c dbSNP:754404198
2944 2944 a, g dbSNP:5030329
2947 2947 c, t dbSNP:747653324
2950 2950 a, g dbSNP:539117133
2974 2974 a, t dbSNP:534396876

Target ORF information:

RefSeq Version NM_024424
Organism Homo sapiens (human)
Definition Homo sapiens Wilms tumor 1 (WT1), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_024424

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG AGTTGCTGCT
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACAGCAC AGGGTACGAG
AGCGATAACC ACACAACGCC CATCCTCTGC GGAGCCCAAT ACAGAATACA CACGCACGGT
GTCTTCAGAG GCATTCAGGA TGTGCGACGT GTGCCTGGAG TAGCCCCGAC TCTTGTACGG
TCGGCATCTG AGACCAGTGA GAAACGCCCC TTCATGTGTG CTTACCCAGG CTGCAATAAG
AGATATTTTA AGCTGTCCCA CTTACAGATG CACAGCAGGA AGCACACTGG TGAGAAACCA
TACCAGTGTG ACTTCAAGGA CTGTGAACGA AGGTTTTCTC GTTCAGACCA GCTCAAAAGA
CACCAAAGGA GACATACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTC
TCCCGGTCCG ACCACCTGAA GACCCACACC AGGACTCATA CAGGTGAAAA GCCCTTCAGC
TGTCGGTGGC CAAGTTGTCA GAAAAAGTTT GCCCGGTCAG ATGAATTAGT CCGCCATCAC
AACATGCATC AGAGAAACAT GACCAAACTC CAGCTGGCGC TTTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu27290D
Sequence Information ORF Nucleotide Sequence (Length: 867bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product Wilms tumor protein isoform F
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG718348.1, AK093168.1, M30393.1 and BC046461.1. Summary: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]. Transcript Variant: This variant (F) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant D. This variant also lacks an alternate, in-frame exon compared to variant D. The encoded isoform (F) has a distinct N-terminus and is shorter than isoform D. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK093168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA
GCCACCTTAA AGGGCCACAG CACAGGGTAC GAGAGCGATA ACCACACAAC GCCCATCCTC
TGCGGAGCCC AATACAGAAT ACACACGCAC GGTGTCTTCA GAGGCATTCA GGATGTGCGA
CGTGTGCCTG GAGTAGCCCC GACTCTTGTA CGGTCGGCAT CTGAGACCAG TGAGAAACGC
CCCTTCATGT GTGCTTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG
ATGCACAGCA GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGTGAA
CGAAGGTTTT CTCGTTCAGA CCAGCTCAAA AGACACCAAA GGAGACATAC AGGTGTGAAA
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTCTCCCGGT CCGACCACCT GAAGACCCAC
ACCAGGACTC ATACAGGTAA AACAAGTGAA AAGCCCTTCA GCTGTCGGTG GCCAAGTTGT
CAGAAAAAGT TTGCCCGGTC AGATGAATTA GTCCGCCATC ACAACATGCA TCAGAGAAAC
ATGACCAAAC TCCAGCTGGC GCTTTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001185481.1
CDS279..1145
Misc Feature(1)231..233(+)
Misc Feature(2)285..758(+)
Misc Feature(3)732..>1118(+)
Misc Feature(4)777..836(+)
Misc Feature(5)783..836(+)
Misc Feature(6)789..1007(+)
Misc Feature(7)810..893(+)
Misc Feature(8)858..926(+)
Misc Feature(9)858..923(+)
Misc Feature(10)903..977(+)
Misc Feature(11)948..1010(+)
Misc Feature(12)948..1010(+)
Misc Feature(13)948..1010(+)
Misc Feature(14)948..1010(+)
Misc Feature(15)963..1106(+)
Misc Feature(16)1041..1109(+)
Misc Feature(17)1041..1109(+)
Misc Feature(18)637..637(+)
Exon (1)1..288
Gene:WT1
Gene Synonym:
Exon (2)289..411
Gene:WT1
Gene Synonym:
Exon (3)412..514
Gene:WT1
Gene Synonym:
Exon (4)515..592
Gene:WT1
Gene Synonym:
Exon (5)593..689
Gene:WT1
Gene Synonym:
Exon (6)690..840
Gene:WT1
Gene Synonym:
Exon (7)841..930
Gene:WT1
Gene Synonym:
Exon (8)931..1023
Gene:WT1
Gene Synonym:
Exon (9)1024..2428
Gene:WT1
Gene Synonym:
Translation
Position Chain Variation Link
4 4 c, g dbSNP:762096406
25 25 c, g dbSNP:565551027
26 26 a, g dbSNP:75870920
78 78 c, t dbSNP:557323865
84 84 c, g dbSNP:148234826
122 122 a, g dbSNP:577579117
123 123 -, g dbSNP:76500597
153 153 a, g dbSNP:536745067
200 200 g, t dbSNP:574176186
202 202 c, t dbSNP:766680590
219 219 a, g dbSNP:554252347
234 234 g, t dbSNP:760438749
239 239 a, g dbSNP:775453593
240 240 c, t dbSNP:771938327
242 242 a, c dbSNP:534172168
248 248 c, t dbSNP:773408487
257 257 c, t dbSNP:770062804
260 260 c, t dbSNP:748347306
268 268 c, t dbSNP:781316935
271 271 -, gc dbSNP:71873106
289 289 a, g dbSNP:369444006
291 291 c, t dbSNP:746367691
298 298 c, t dbSNP:779451877
299 299 c, g dbSNP:756695525
301 301 c, t dbSNP:576902446
304 304 a, c, t dbSNP:556804456
305 305 c, g dbSNP:752438619
312 312 a, g dbSNP:767419243
313 313 c, g dbSNP:759464627
314 314 c, g dbSNP:760001898
316 316 c, t dbSNP:777166391
318 318 c, t dbSNP:766425764
322 322 c, g dbSNP:761913397
323 323 c, t dbSNP:9332974
335 335 a, g dbSNP:768828897
337 337 c, t dbSNP:760776653
338 338 c, t dbSNP:183263057
341 341 g, t dbSNP:772409488
344 344 c, g dbSNP:746241159
350 350 a, g dbSNP:779321799
357 357 a, t dbSNP:771455202
367 367 a, t dbSNP:749860238
373 373 a, t dbSNP:777119782
387 387 a, c, t dbSNP:2234584
390 390 a, g dbSNP:142653301
391 391 a, t dbSNP:377573993
393 393 a, g dbSNP:751432306
412 412 a, g dbSNP:372225738
430 430 c, t dbSNP:765230888
433 433 c, g dbSNP:756078681
438 438 c, t dbSNP:752801263
443 443 a, c, g dbSNP:536750501
445 445 a, g dbSNP:774626752
446 446 c, t dbSNP:766642594
452 452 c, g dbSNP:200222400
457 457 c, t dbSNP:138073760
459 459 c, g dbSNP:773527284
463 463 c, t dbSNP:770366595
464 464 c, g dbSNP:747613465
475 475 c, t dbSNP:776258782
476 476 c, t dbSNP:145425799
479 479 c, g dbSNP:548704325
481 481 a, g dbSNP:779813097
486 486 g, t dbSNP:368452754
520 520 -, atttataccaaatgaca dbSNP:587776573
522 522 a, t dbSNP:780092599
525 525 c, t dbSNP:758624956
527 527 c, t dbSNP:190440703
528 528 c, t dbSNP:547775568
534 534 a, g dbSNP:762325377
538 538 c, t dbSNP:267602852
552 552 a, g dbSNP:754336808
566 566 c, g dbSNP:764701739
568 568 c, t dbSNP:760219458
572 572 c, t dbSNP:775085343
583 583 a, c dbSNP:372630828
596 596 c, t dbSNP:375114529
598 598 a, g dbSNP:371021920
605 605 a, g dbSNP:752102247
609 609 a, g dbSNP:547783372
611 611 a, g dbSNP:757962229
612 612 a, g dbSNP:121907908
614 614 c, t dbSNP:750018485
615 615 a, g dbSNP:267602851
625 625 c, t dbSNP:764802290
628 628 a, c dbSNP:761627098
634 634 a, t dbSNP:548544187
635 635 c, t dbSNP:527655625
639 639 c, t dbSNP:142059681
643 643 a, g dbSNP:760654956
647 647 c, g dbSNP:775630812
648 648 c, g dbSNP:771117558
650 650 a, g dbSNP:2234590
652 652 a, g dbSNP:773532792
659 659 a, g dbSNP:17855567
668 668 a, c dbSNP:587778756
670 670 a, g dbSNP:769934402
670 670 -, g dbSNP:587776574
676 676 a, g, t dbSNP:150194429
680 680 a, t dbSNP:544966826
687 687 a, c dbSNP:747502066
693 693 a, g dbSNP:747377024
695 695 a, g dbSNP:780497248
697 697 a, g dbSNP:772590420
698 698 a, g dbSNP:16754
700 700 a, c, g, t dbSNP:554416372
707 707 c, t dbSNP:151034312
711 711 a, g dbSNP:755936509
716 716 a, c, t dbSNP:147126640
717 717 c, t dbSNP:754938612
722 722 a, t dbSNP:371168589
729 729 c, t dbSNP:765218093
730 730 a, g dbSNP:147241955
731 731 g, t dbSNP:776651253
733 733 c, g dbSNP:142937387
734 734 a, g dbSNP:377446096
737 737 a, g dbSNP:141834493
758 758 c, t dbSNP:147939483
759 759 c, t dbSNP:267602850
764 764 c, t dbSNP:200539928
774 774 c, t dbSNP:746353651
784 784 a, g dbSNP:121907904
786 786 a, c dbSNP:774976881
787 787 a, c, g dbSNP:748864758
829 829 g, t dbSNP:373176048
845 845 a, g dbSNP:767850636
854 854 c, t dbSNP:759955361
857 857 c, g dbSNP:774650640
861 861 a, g dbSNP:771494968
873 873 g, t dbSNP:121907905
879 879 c, t dbSNP:121907906
880 880 a, g dbSNP:144788858
882 882 a, g dbSNP:77183751
891 891 c, t dbSNP:121907910
892 892 a, g dbSNP:121907901
914 914 c, g dbSNP:121907907
917 917 a, t dbSNP:772822466
922 922 a, g dbSNP:769499906
924 924 c, t dbSNP:28942089
942 942 c, t dbSNP:28941777
947 947 a, g dbSNP:546743811
954 954 a, g dbSNP:751932589
955 955 c, t dbSNP:766697865
961 961 a, t dbSNP:533043871
963 963 c, t dbSNP:121907909
969 969 c, t dbSNP:28941779
975 975 c, t dbSNP:121907900
976 976 c, g dbSNP:121907903
980 980 c, t dbSNP:374799820
981 981 a, g dbSNP:28941778
982 982 a, g dbSNP:121907902
995 995 c, t dbSNP:769371345
1010 1010 c, t dbSNP:761414130
1039 1039 a, g dbSNP:267602849
1045 1045 a, g dbSNP:139893274
1050 1050 c, t dbSNP:761360324
1053 1053 a, g dbSNP:776256676
1054 1054 c, g dbSNP:763551837
1063 1063 a, g dbSNP:760370132
1103 1103 c, g dbSNP:369940913
1120 1120 c, t dbSNP:775063171
1123 1123 c, t dbSNP:528613270
1127 1127 a, c dbSNP:745663070
1130 1130 c, t dbSNP:267602848
1135 1135 c, t dbSNP:774228907
1138 1138 a, c, t dbSNP:749266841
1139 1139 a, g dbSNP:202118284
1144 1144 a, g dbSNP:148856160
1146 1146 a, g dbSNP:747197306
1147 1147 a, g, t dbSNP:758798048
1149 1149 c, g dbSNP:750842875
1150 1150 c, t dbSNP:765743928
1155 1155 c, t dbSNP:757807247
1157 1157 c, t dbSNP:77462662
1158 1158 a, g dbSNP:763645824
1161 1161 g, t dbSNP:2234594
1165 1165 a, g, t dbSNP:767103760
1174 1174 c, t dbSNP:759275928
1175 1175 c, t dbSNP:774104210
1180 1180 a, c dbSNP:376972948
1187 1187 a, g dbSNP:749213891
1191 1191 a, g dbSNP:773040516
1226 1226 c, t dbSNP:563643335
1229 1229 c, g dbSNP:749749213
1233 1233 a, g dbSNP:5030315
1255 1255 c, t dbSNP:757474299
1283 1283 a, g dbSNP:111351882
1285 1285 c, t dbSNP:770485644
1304 1304 c, g dbSNP:746218880
1353 1353 c, t dbSNP:560907250
1369 1369 a, g dbSNP:5030316
1412 1412 c, g dbSNP:5030317
1425 1425 g, t dbSNP:181881769
1484 1484 a, g dbSNP:544612574
1490 1490 a, g dbSNP:5030318
1510 1510 a, t dbSNP:557682653
1523 1523 a, c dbSNP:537329306
1524 1524 c, t dbSNP:746694121
1535 1535 a, t dbSNP:568703873
1536 1536 a, c dbSNP:777105291
1565 1565 g, t dbSNP:772298084
1574 1574 g, t dbSNP:554888133
1598 1598 c, g dbSNP:761025226
1604 1604 a, g dbSNP:535181585
1610 1610 a, c dbSNP:189407871
1621 1621 a, g dbSNP:757906937
1658 1658 c, t dbSNP:575602262
1659 1659 a, g dbSNP:372622383
1666 1666 a, g dbSNP:765145800
1687 1687 a, g dbSNP:142726499
1710 1710 a, t dbSNP:754974695
1715 1715 -, t dbSNP:774867727
1718 1718 -, g dbSNP:79335260
1719 1719 -, g dbSNP:5030319
1747 1747 a, g dbSNP:5030320
1749 1749 a, c dbSNP:766220081
1752 1752 a, g dbSNP:550080671
1797 1797 a, t dbSNP:761903425
1811 1811 a, g dbSNP:374306749
1814 1814 a, t dbSNP:764209237
1848 1848 a, g dbSNP:763107748
1919 1919 a, c dbSNP:184847211
1937 1937 a, g dbSNP:746314166
1942 1942 a, g dbSNP:148478722
1965 1965 c, g dbSNP:1042347
1980 1980 a, g dbSNP:5030322
2009 2009 a, g, t dbSNP:5030323
2017 2017 c, t dbSNP:576023116
2042 2042 c, g dbSNP:5030324
2048 2048 a, g dbSNP:5030325
2058 2058 c, t dbSNP:760142967
2094 2094 a, c dbSNP:776814761
2100 2100 a, g dbSNP:771360373
2117 2117 -, a dbSNP:72519751
2123 2123 -, a dbSNP:5030326
2147 2147 a, c dbSNP:575035138
2165 2165 -, cgtg dbSNP:780961917
2166 2166 -, gtgt dbSNP:58549495
2188 2188 -, gtgtg dbSNP:770524174
2190 2190 -, gtgtg dbSNP:758276052
2190 2190 -, gtg dbSNP:766176703
2192 2192 -, gtg dbSNP:750046431
2194 2194 -, gtgtgt dbSNP:147708114
2194 2194 -, gtgt dbSNP:761038390
2194 2194 -, g dbSNP:568803808
2195 2195 -, tgtgtt dbSNP:754104430
2197 2197 -, tgtt dbSNP:767995702
2198 2198 -, gt dbSNP:200382866
2207 2207 (tg)19, 20, 21, 22, 23 dbSNP:3138550
2243 2243 c, t dbSNP:5030327
2244 2244 a, g dbSNP:746578571
2277 2277 a, t dbSNP:5030328
2286 2286 a, t dbSNP:566276801
2312 2312 g, t dbSNP:771770230
2318 2318 a, c dbSNP:754404198
2354 2354 a, g dbSNP:5030329
2357 2357 c, t dbSNP:747653324
2360 2360 a, g dbSNP:539117133
2384 2384 a, t dbSNP:534396876

Target ORF information:

RefSeq Version NM_001198552
Organism Homo sapiens (human)
Definition Homo sapiens Wilms tumor 1 (WT1), transcript variant F, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001198552

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA
GCCACCTTAA AGGGCCACAG CACAGGGTAC GAGAGCGATA ACCACACAAC GCCCATCCTC
TGCGGAGCCC AATACAGAAT ACACACGCAC GGTGTCTTCA GAGGCATTCA GGATGTGCGA
CGTGTGCCTG GAGTAGCCCC GACTCTTGTA CGGTCGGCAT CTGAGACCAG TGAGAAACGC
CCCTTCATGT GTGCTTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG
ATGCACAGCA GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGTGAA
CGAAGGTTTT CTCGTTCAGA CCAGCTCAAA AGACACCAAA GGAGACATAC AGGTGTGAAA
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTCTCCCGGT CCGACCACCT GAAGACCCAC
ACCAGGACTC ATACAGGTAA AACAAGTGAA AAGCCCTTCA GCTGTCGGTG GCCAAGTTGT
CAGAAAAAGT TTGCCCGGTC AGATGAATTA GTCCGCCATC ACAACATGCA TCAGAGAAAC
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu27351D
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product Wilms tumor protein isoform D
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK291736.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Oct 23, 2010 this sequence version replaced gi:65508003. Summary: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]. Transcript Variant: This variant (D) represents the longest transcript and encodes the longest isoform (D). It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X51630.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG AGTTGCTGCT
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACAGCAC AGGGTACGAG
AGCGATAACC ACACAACGCC CATCCTCTGC GGAGCCCAAT ACAGAATACA CACGCACGGT
GTCTTCAGAG GCATTCAGGA TGTGCGACGT GTGCCTGGAG TAGCCCCGAC TCTTGTACGG
TCGGCATCTG AGACCAGTGA GAAACGCCCC TTCATGTGTG CTTACCCAGG CTGCAATAAG
AGATATTTTA AGCTGTCCCA CTTACAGATG CACAGCAGGA AGCACACTGG TGAGAAACCA
TACCAGTGTG ACTTCAAGGA CTGTGAACGA AGGTTTTCTC GTTCAGACCA GCTCAAAAGA
CACCAAAGGA GACATACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTC
TCCCGGTCCG ACCACCTGAA GACCCACACC AGGACTCATA CAGGTAAAAC AAGTGAAAAG
CCCTTCAGCT GTCGGTGGCC AAGTTGTCAG AAAAAGTTTG CCCGGTCAGA TGAATTAGTC
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_077744.3
CDS191..1744
Misc Feature(1)395..1357(+)
Misc Feature(2)1331..>1717(+)
Misc Feature(3)1376..1435(+)
Misc Feature(4)1382..1435(+)
Misc Feature(5)1388..1606(+)
Misc Feature(6)1409..1492(+)
Misc Feature(7)1457..1525(+)
Misc Feature(8)1457..1522(+)
Misc Feature(9)1487..1489(+)
Misc Feature(10)1502..1576(+)
Misc Feature(11)1547..1609(+)
Misc Feature(12)1547..1609(+)
Misc Feature(13)1547..1609(+)
Misc Feature(14)1547..1609(+)
Misc Feature(15)1562..1705(+)
Misc Feature(16)1571..1573(+)
Misc Feature(17)1640..1708(+)
Misc Feature(18)1640..1708(+)
Misc Feature(19)395..397(+)
Misc Feature(20)1236..1236(+)
Exon (1)1..836
Gene:WT1
Gene Synonym:
Exon (2)837..959
Gene:WT1
Gene Synonym:
Exon (3)960..1062
Gene:WT1
Gene Synonym:
Exon (4)1063..1140
Gene:WT1
Gene Synonym:
Exon (5)1141..1191
Gene:WT1
Gene Synonym:
Exon (6)1192..1288
Gene:WT1
Gene Synonym:
Exon (7)1289..1439
Gene:WT1
Gene Synonym:
Exon (8)1440..1529
Gene:WT1
Gene Synonym:
Exon (9)1530..1622
Gene:WT1
Gene Synonym:
Exon (10)1623..3027
Gene:WT1
Gene Synonym:
Translation
Position Chain Variation Link
14 14 a, c dbSNP:772399951
54 54 c, g dbSNP:541850548
101 101 c, t dbSNP:5030133
113 113 c, t dbSNP:572838503
114 114 a, c dbSNP:552828543
115 115 c, t dbSNP:539323571
117 117 a, c dbSNP:570325451
119 119 -, ccca dbSNP:542898204
202 202 a, g dbSNP:556835183
209 209 a, c dbSNP:764111950
256 256 c, t dbSNP:755007049
258 258 a, g dbSNP:751641518
277 277 a, g dbSNP:766527734
290 290 c, t dbSNP:763147828
292 292 a, g dbSNP:537150542
297 297 c, t dbSNP:773550149
298 298 c, g dbSNP:555140661
299 299 a, g dbSNP:762288656
326 326 -, g dbSNP:776155094
337 337 c, g dbSNP:776954184
356 356 a, c, g dbSNP:2234581
368 368 a, g dbSNP:374404615
376 376 c, g dbSNP:771769393
385 385 g, t dbSNP:745506215
388 388 g, t dbSNP:2234582
391 391 g, t dbSNP:5030135
398 398 a, g dbSNP:528076586
416 416 c, g dbSNP:777617460
425 425 c, t dbSNP:756055892
434 434 a, g dbSNP:752686863
445 445 c, g dbSNP:766441234
455 455 a, g dbSNP:758512604
461 461 -, ggc dbSNP:770519620
466 466 g, t dbSNP:750588084
473 473 c, t dbSNP:567200038
484 484 a, c dbSNP:547333427
491 491 a, c dbSNP:762044578
502 502 c, g dbSNP:777059515
520 520 c, t dbSNP:1799925
528 528 c, t dbSNP:761252308
550 550 c, t dbSNP:776209354
556 556 c, g dbSNP:771681406
561 561 c, t dbSNP:745435848
566 566 a, c, t dbSNP:564706633
569 569 a, g dbSNP:773831663
573 573 c, t dbSNP:770618081
576 576 a, c dbSNP:748974419
577 577 a, g dbSNP:777527675
579 579 c, t dbSNP:769642496
581 581 a, c dbSNP:748045691
587 587 c, t dbSNP:544477985
592 592 a, g dbSNP:758361767
595 595 -, gcc dbSNP:772893663
596 596 c, t dbSNP:750548251
597 597 -, gcc dbSNP:760304811
602 602 a, t dbSNP:778937284
604 604 c, g dbSNP:531076591
607 607 c, t dbSNP:754173303
615 615 a, g dbSNP:764552529
619 619 a, g dbSNP:761166030
646 646 c, t dbSNP:753238865
650 650 a, g dbSNP:768165877
655 655 a, g dbSNP:758900425
658 658 c, t dbSNP:773922379
659 659 c, t dbSNP:770403127
678 678 a, g dbSNP:762660918
685 685 c, t dbSNP:772890986
688 688 a, c dbSNP:536728682
697 697 c, t dbSNP:747958111
718 718 c, t dbSNP:369870529
721 721 a, c dbSNP:121907911
731 731 g, t dbSNP:768644520
741 741 c, g dbSNP:745879965
742 742 g, t dbSNP:141135091
744 744 c, t dbSNP:757349907
753 753 c, t dbSNP:572926487
759 759 c, g dbSNP:778194188
761 761 a, g dbSNP:756501972
762 762 a, g dbSNP:753112302
766 766 a, g dbSNP:767850217
767 767 a, g, t dbSNP:377072761
771 771 c, g dbSNP:765879407
772 772 a, g dbSNP:762418497
784 784 c, t dbSNP:2234583
785 785 a, g dbSNP:9332973
790 790 c, g dbSNP:769467940
802 802 c, t dbSNP:761530541
803 803 g, t dbSNP:776426005
808 808 c, t dbSNP:576677344
813 813 a, g dbSNP:746964440
814 814 a, c dbSNP:775333473
816 816 a, c dbSNP:771024882
820 820 a, c dbSNP:749405227
821 821 a, g dbSNP:778069787
828 828 a, g dbSNP:756414084
830 830 a, c dbSNP:748435329
833 833 a, c, g dbSNP:373935628
837 837 a, g dbSNP:369444006
839 839 c, t dbSNP:746367691
846 846 c, t dbSNP:779451877
847 847 c, g dbSNP:756695525
849 849 c, t dbSNP:576902446
852 852 a, c, t dbSNP:556804456
853 853 c, g dbSNP:752438619
860 860 a, g dbSNP:767419243
861 861 c, g dbSNP:759464627
862 862 c, g dbSNP:760001898
864 864 c, t dbSNP:777166391
866 866 c, t dbSNP:766425764
870 870 c, g dbSNP:761913397
871 871 c, t dbSNP:9332974
883 883 a, g dbSNP:768828897
885 885 c, t dbSNP:760776653
886 886 c, t dbSNP:183263057
889 889 g, t dbSNP:772409488
892 892 c, g dbSNP:746241159
898 898 a, g dbSNP:779321799
905 905 a, t dbSNP:771455202
915 915 a, t dbSNP:749860238
921 921 a, t dbSNP:777119782
935 935 a, c, t dbSNP:2234584
938 938 a, g dbSNP:142653301
939 939 a, t dbSNP:377573993
941 941 a, g dbSNP:751432306
960 960 a, g dbSNP:372225738
978 978 c, t dbSNP:765230888
981 981 c, g dbSNP:756078681
986 986 c, t dbSNP:752801263
991 991 a, c, g dbSNP:536750501
993 993 a, g dbSNP:774626752
994 994 c, t dbSNP:766642594
1000 1000 c, g dbSNP:200222400
1005 1005 c, t dbSNP:138073760
1007 1007 c, g dbSNP:773527284
1011 1011 c, t dbSNP:770366595
1012 1012 c, g dbSNP:747613465
1023 1023 c, t dbSNP:776258782
1024 1024 c, t dbSNP:145425799
1027 1027 c, g dbSNP:548704325
1029 1029 a, g dbSNP:779813097
1034 1034 g, t dbSNP:368452754
1068 1068 -, atttataccaaatgaca dbSNP:587776573
1070 1070 a, t dbSNP:780092599
1073 1073 c, t dbSNP:758624956
1075 1075 c, t dbSNP:190440703
1076 1076 c, t dbSNP:547775568
1082 1082 a, g dbSNP:762325377
1086 1086 c, t dbSNP:267602852
1100 1100 a, g dbSNP:754336808
1114 1114 c, g dbSNP:764701739
1116 1116 c, t dbSNP:760219458
1120 1120 c, t dbSNP:775085343
1131 1131 a, c dbSNP:372630828
1141 1141 a, c dbSNP:776902132
1149 1149 -, ctg dbSNP:769982733
1152 1152 c, g dbSNP:766054482
1156 1156 c, t dbSNP:371218185
1164 1164 c, t dbSNP:762688982
1171 1171 a, g dbSNP:758410591
1185 1185 a, g dbSNP:769618028
1188 1188 a, g dbSNP:748112905
1195 1195 c, t dbSNP:375114529
1197 1197 a, g dbSNP:371021920
1204 1204 a, g dbSNP:752102247
1208 1208 a, g dbSNP:547783372
1210 1210 a, g dbSNP:757962229
1211 1211 a, g dbSNP:121907908
1213 1213 c, t dbSNP:750018485
1214 1214 a, g dbSNP:267602851
1224 1224 c, t dbSNP:764802290
1227 1227 a, c dbSNP:761627098
1233 1233 a, t dbSNP:548544187
1234 1234 c, t dbSNP:527655625
1238 1238 c, t dbSNP:142059681
1242 1242 a, g dbSNP:760654956
1246 1246 c, g dbSNP:775630812
1247 1247 c, g dbSNP:771117558
1249 1249 a, g dbSNP:2234590
1251 1251 a, g dbSNP:773532792
1258 1258 a, g dbSNP:17855567
1267 1267 a, c dbSNP:587778756
1269 1269 a, g dbSNP:769934402
1269 1269 -, g dbSNP:587776574
1275 1275 a, g, t dbSNP:150194429
1279 1279 a, t dbSNP:544966826
1286 1286 a, c dbSNP:747502066
1292 1292 a, g dbSNP:747377024
1294 1294 a, g dbSNP:780497248
1296 1296 a, g dbSNP:772590420
1297 1297 a, g dbSNP:16754
1299 1299 a, c, g, t dbSNP:554416372
1306 1306 c, t dbSNP:151034312
1310 1310 a, g dbSNP:755936509
1315 1315 a, c, t dbSNP:147126640
1316 1316 c, t dbSNP:754938612
1321 1321 a, t dbSNP:371168589
1328 1328 c, t dbSNP:765218093
1329 1329 a, g dbSNP:147241955
1330 1330 g, t dbSNP:776651253
1332 1332 c, g dbSNP:142937387
1333 1333 a, g dbSNP:377446096
1336 1336 a, g dbSNP:141834493
1357 1357 c, t dbSNP:147939483
1358 1358 c, t dbSNP:267602850
1363 1363 c, t dbSNP:200539928
1373 1373 c, t dbSNP:746353651
1383 1383 a, g dbSNP:121907904
1385 1385 a, c dbSNP:774976881
1386 1386 a, c, g dbSNP:748864758
1428 1428 g, t dbSNP:373176048
1444 1444 a, g dbSNP:767850636
1453 1453 c, t dbSNP:759955361
1456 1456 c, g dbSNP:774650640
1460 1460 a, g dbSNP:771494968
1472 1472 g, t dbSNP:121907905
1478 1478 c, t dbSNP:121907906
1479 1479 a, g dbSNP:144788858
1481 1481 a, g dbSNP:77183751
1490 1490 c, t dbSNP:121907910
1491 1491 a, g dbSNP:121907901
1513 1513 c, g dbSNP:121907907
1516 1516 a, t dbSNP:772822466
1521 1521 a, g dbSNP:769499906
1523 1523 c, t dbSNP:28942089
1541 1541 c, t dbSNP:28941777
1546 1546 a, g dbSNP:546743811
1553 1553 a, g dbSNP:751932589
1554 1554 c, t dbSNP:766697865
1560 1560 a, t dbSNP:533043871
1562 1562 c, t dbSNP:121907909
1568 1568 c, t dbSNP:28941779
1574 1574 c, t dbSNP:121907900
1575 1575 c, g dbSNP:121907903
1579 1579 c, t dbSNP:374799820
1580 1580 a, g dbSNP:28941778
1581 1581 a, g dbSNP:121907902
1594 1594 c, t dbSNP:769371345
1609 1609 c, t dbSNP:761414130
1638 1638 a, g dbSNP:267602849
1644 1644 a, g dbSNP:139893274
1649 1649 c, t dbSNP:761360324
1652 1652 a, g dbSNP:776256676
1653 1653 c, g dbSNP:763551837
1662 1662 a, g dbSNP:760370132
1702 1702 c, g dbSNP:369940913
1719 1719 c, t dbSNP:775063171
1722 1722 c, t dbSNP:528613270
1726 1726 a, c dbSNP:745663070
1729 1729 c, t dbSNP:267602848
1734 1734 c, t dbSNP:774228907
1737 1737 a, c, t dbSNP:749266841
1738 1738 a, g dbSNP:202118284
1743 1743 a, g dbSNP:148856160
1745 1745 a, g dbSNP:747197306
1746 1746 a, g, t dbSNP:758798048
1748 1748 c, g dbSNP:750842875
1749 1749 c, t dbSNP:765743928
1754 1754 c, t dbSNP:757807247
1756 1756 c, t dbSNP:77462662
1757 1757 a, g dbSNP:763645824
1760 1760 g, t dbSNP:2234594
1764 1764 a, g, t dbSNP:767103760
1773 1773 c, t dbSNP:759275928
1774 1774 c, t dbSNP:774104210
1779 1779 a, c dbSNP:376972948
1786 1786 a, g dbSNP:749213891
1790 1790 a, g dbSNP:773040516
1825 1825 c, t dbSNP:563643335
1828 1828 c, g dbSNP:749749213
1832 1832 a, g dbSNP:5030315
1854 1854 c, t dbSNP:757474299
1882 1882 a, g dbSNP:111351882
1884 1884 c, t dbSNP:770485644
1903 1903 c, g dbSNP:746218880
1952 1952 c, t dbSNP:560907250
1968 1968 a, g dbSNP:5030316
2011 2011 c, g dbSNP:5030317
2024 2024 g, t dbSNP:181881769
2083 2083 a, g dbSNP:544612574
2089 2089 a, g dbSNP:5030318
2109 2109 a, t dbSNP:557682653
2122 2122 a, c dbSNP:537329306
2123 2123 c, t dbSNP:746694121
2134 2134 a, t dbSNP:568703873
2135 2135 a, c dbSNP:777105291
2164 2164 g, t dbSNP:772298084
2173 2173 g, t dbSNP:554888133
2197 2197 c, g dbSNP:761025226
2203 2203 a, g dbSNP:535181585
2209 2209 a, c dbSNP:189407871
2220 2220 a, g dbSNP:757906937
2257 2257 c, t dbSNP:575602262
2258 2258 a, g dbSNP:372622383
2265 2265 a, g dbSNP:765145800
2286 2286 a, g dbSNP:142726499
2309 2309 a, t dbSNP:754974695
2314 2314 -, t dbSNP:774867727
2317 2317 -, g dbSNP:79335260
2318 2318 -, g dbSNP:5030319
2346 2346 a, g dbSNP:5030320
2348 2348 a, c dbSNP:766220081
2351 2351 a, g dbSNP:550080671
2396 2396 a, t dbSNP:761903425
2410 2410 a, g dbSNP:374306749
2413 2413 a, t dbSNP:764209237
2447 2447 a, g dbSNP:763107748
2518 2518 a, c dbSNP:184847211
2536 2536 a, g dbSNP:746314166
2541 2541 a, g dbSNP:148478722
2564 2564 c, g dbSNP:1042347
2579 2579 a, g dbSNP:5030322
2608 2608 a, g, t dbSNP:5030323
2616 2616 c, t dbSNP:576023116
2641 2641 c, g dbSNP:5030324
2647 2647 a, g dbSNP:5030325
2657 2657 c, t dbSNP:760142967
2693 2693 a, c dbSNP:776814761
2699 2699 a, g dbSNP:771360373
2716 2716 -, a dbSNP:72519751
2722 2722 -, a dbSNP:5030326
2746 2746 a, c dbSNP:575035138
2764 2764 -, cgtg dbSNP:780961917
2765 2765 -, gtgt dbSNP:58549495
2787 2787 -, gtgtg dbSNP:770524174
2789 2789 -, gtgtg dbSNP:758276052
2789 2789 -, gtg dbSNP:766176703
2791 2791 -, gtg dbSNP:750046431
2793 2793 -, gtgtgt dbSNP:147708114
2793 2793 -, gtgt dbSNP:761038390
2793 2793 -, g dbSNP:568803808
2794 2794 -, tgtgtt dbSNP:754104430
2796 2796 -, tgtt dbSNP:767995702
2797 2797 -, gt dbSNP:200382866
2806 2806 (tg)19, 20, 21, 22, 23 dbSNP:3138550
2842 2842 c, t dbSNP:5030327
2843 2843 a, g dbSNP:746578571
2876 2876 a, t dbSNP:5030328
2885 2885 a, t dbSNP:566276801
2911 2911 g, t dbSNP:771770230
2917 2917 a, c dbSNP:754404198
2953 2953 a, g dbSNP:5030329
2956 2956 c, t dbSNP:747653324
2959 2959 a, g dbSNP:539117133
2983 2983 a, t dbSNP:534396876

Target ORF information:

RefSeq Version NM_024426
Organism Homo sapiens (human)
Definition Homo sapiens Wilms tumor 1 (WT1), transcript variant D, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_024426

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG AGTTGCTGCT
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACAGCAC AGGGTACGAG
AGCGATAACC ACACAACGCC CATCCTCTGC GGAGCCCAAT ACAGAATACA CACGCACGGT
GTCTTCAGAG GCATTCAGGA TGTGCGACGT GTGCCTGGAG TAGCCCCGAC TCTTGTACGG
TCGGCATCTG AGACCAGTGA GAAACGCCCC TTCATGTGTG CTTACCCAGG CTGCAATAAG
AGATATTTTA AGCTGTCCCA CTTACAGATG CACAGCAGGA AGCACACTGG TGAGAAACCA
TACCAGTGTG ACTTCAAGGA CTGTGAACGA AGGTTTTCTC GTTCAGACCA GCTCAAAAGA
CACCAAAGGA GACATACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTC
TCCCGGTCCG ACCACCTGAA GACCCACACC AGGACTCATA CAGGTAAAAC AAGTGAAAAG
CCCTTCAGCT GTCGGTGGCC AAGTTGTCAG AAAAAGTTTG CCCGGTCAGA TGAATTAGTC
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.