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ZP3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol ZP3
Entrez Gene ID 7784
Full Name zona pellucida glycoprotein 3 (sperm receptor)
Synonyms ZP3A, ZP3B, ZPC, Zp-3
General protein information
Preferred Names
zona pellucida sperm-binding protein 3
Names
zona pellucida sperm-binding protein 3
ZP3A/ZP3B
sperm receptor
zona pellucida protein C
zona pellucida glycoprotein 3B
zona pellucida glycoprotein ZP3
zona pellucida glycoprotein 3A (sperm receptor)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q11.23

Summary The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

182889


mRNA and Protein(s)

mRNA Protein Name
NM_007155 NP_009086 zona pellucida sperm-binding protein 3 isoform 2
NM_001110354 NP_001103824 zona pellucida sperm-binding protein 3 isoform 1 precursor



Homo sapiens (human) ZP3 NP_001103824.1
Pan troglodytes (chimpanzee) ZP3 XP_528035.3
Macaca mulatta (Rhesus monkey) ZP3 XP_002803282.1
Canis lupus familiaris (dog) ZP3 NP_001003224.1
Bos taurus (cattle) ZP3 NP_776399.1
Mus musculus (house mouse) Zp3 NP_035906.1
Rattus norvegicus (Norway rat) Zp3 NP_446214.1
Gallus gallus (chicken) ZP3L2 XP_413701.4
Gallus gallus (chicken) ZP3 NP_989720.3
Gallus gallus (chicken) ZP3L1 XP_413693.2
Danio rerio (zebrafish) LOC100536860 XP_003200340.1
Danio rerio (zebrafish) zp3a.1 NP_001013289.1
Danio rerio (zebrafish) zp3a.2 NP_001025291.1
Danio rerio (zebrafish) LOC100148225 XP_005169843.1
Danio rerio (zebrafish) zgc:173556 NP_001103212.1
Danio rerio (zebrafish) zp3.2 NP_001020357.1
Danio rerio (zebrafish) zp3 NP_571406.1
Danio rerio (zebrafish) zgc:171779 NP_001103303.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC394444 NP_988853.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100495051 XP_004913254.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ZP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18037 NM_007155 Homo sapiens zona pellucida glycoprotein 3 (sperm receptor) (ZP3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu31830 NM_001110354 Homo sapiens zona pellucida glycoprotein 3 (sperm receptor) (ZP3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18037
Clone ID Related Accession (Same CDS sequence) NM_007155
Accession Version NM_007155.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1122bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product zona pellucida sperm-binding protein 3 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292763.1, DB328313.1 and BC113949.1. This sequence is a reference standard in the RefSeqGene project. On Nov 16, 2007 this sequence version replaced gi:38327648. Transcript Variant: This variant (2) differs in the 5' UTR and 5' CDS compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292763.1, BC113949.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGTCATGG TCAGCAAAGA CCTTTTTGGC ACCGGGAAGC TCATCAGGGC TGCTGACCTC 
ACCTTGGGCC CAGAGGCCTG TGAGCCTCTG GTCTCCATGG ACACAGAAGA TGTGGTCAGG
TTTGAGGTTG GACTCCACGA GTGTGGCAAC AGCATGCAGG TAACTGACGA TGCCCTGGTG
TACAGCACCT TCCTGCTCCA TGACCCCCGC CCCGTGGGAA ACCTGTCCAT CGTGAGGACT
AACCGCGCAG AGATTCCCAT CGAGTGCCGC TACCCCAGGC AGGGCAATGT GAGCAGCCAG
GCCATCCTGC CCACCTGGTT GCCCTTCAGG ACCACGGTGT TCTCAGAGGA GAAGCTGACT
TTCTCTCTGC GTCTGATGGA GGAGAACTGG AACGCTGAGA AGAGGTCCCC CACCTTCCAC
CTGGGAGATG CAGCCCACCT CCAGGCAGAA ATCCACACTG GCAGCCACGT GCCACTGCGG
TTGTTTGTGG ACCACTGCGT GGCCACACCG ACACCAGACC AGAATGCCTC CCCTTATCAC
ACCATCGTGG ACTTCCATGG CTGTCTTGTC GACGGTCTCA CTGATGCCTC TTCTGCATTC
AAAGTTCCTC GACCCGGGCC AGATACACTC CAGTTCACAG TGGATGTCTT CCACTTTGCT
AATGACTCCA GAAACATGAT ATACATCACC TGCCACCTGA AGGTCACCCT AGCTGAGCAG
GACCCAGATG AACTCAACAA GGCCTGTTCC TTCAGCAAGC CTTCCAACAG CTGGTTCCCA
GTGGAAGGCT CGGCTGACAT CTGTCAATGC TGTAACAAAG GTGACTGTGG CACTCCAAGC
CATTCCAGGA GGCAGCCTCA TGTCATGAGC CAGTGGTCCA GGTCTGCTTC CCGTAACCGC
AGGCATGTGA CAGAAGAAGC AGATGTCACC GTGGGGCCAC TGATCTTCCT GGACAGGAGG
GGTGACCATG AAGTAGAGCA GTGGGCTTTG CCTTCTGACA CCTCAGTGGT GCTGCTGGGC
GTAGGCCTGG CTGTGGTGGT GTCCCTGACT CTGACTGCTG TTATCCTGGT TCTCACCAGG
AGGTGTCGCA CTGCCTCCCA CCCTGTGTCT GCTTCCGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_009086.4
CDS341..1462
Misc Feature(1)344..1096(+)
Misc Feature(2)1241..1243(+)
Exon (1)1..274
Gene:ZP3
Gene Synonym:
Exon (2)275..499
Gene:ZP3
Gene Synonym:
Exon (3)500..618
Gene:ZP3
Gene Synonym:
Exon (4)619..722
Gene:ZP3
Gene Synonym:
Exon (5)723..900
Gene:ZP3
Gene Synonym:
Exon (6)901..1018
Gene:ZP3
Gene Synonym:
Exon (7)1019..1110
Gene:ZP3
Gene Synonym:
Exon (8)1111..1247
Gene:ZP3
Gene Synonym:
Exon (9)1248..1477
Gene:ZP3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_007155
Organism Homo sapiens (human)
Definition Homo sapiens zona pellucida glycoprotein 3 (sperm receptor) (ZP3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007155

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGTCATGG TCAGCAAAGA CCTTTTTGGC ACCGGGAAGC TCATCAGGGC TGCTGACCTC 
ACCTTGGGCC CAGAGGCCTG TGAGCCTCTG GTCTCCATGG ACACAGAAGA TGTGGTCAGG
TTTGAGGTTG GACTCCACGA GTGTGGCAAC AGCATGCAGG TAACTGACGA TGCCCTGGTG
TACAGCACCT TCCTGCTCCA TGACCCCCGC CCCGTGGGAA ACCTGTCCAT CGTGAGGACT
AACCGCGCAG AGATTCCCAT CGAGTGCCGC TACCCCAGGC AGGGCAATGT GAGCAGCCAG
GCCATCCTGC CCACCTGGTT GCCCTTCAGG ACCACGGTGT TCTCAGAGGA GAAGCTGACT
TTCTCTCTGC GTCTGATGGA GGAGAACTGG AACGCTGAGA AGAGGTCCCC CACCTTCCAC
CTGGGAGATG CAGCCCACCT CCAGGCAGAA ATCCACACTG GCAGCCACGT GCCACTGCGG
TTGTTTGTGG ACCACTGCGT GGCCACACCG ACACCAGACC AGAATGCCTC CCCTTATCAC
ACCATCGTGG ACTTCCATGG CTGTCTTGTC GACGGTCTCA CTGATGCCTC TTCTGCATTC
AAAGTTCCTC GACCCGGGCC AGATACACTC CAGTTCACAG TGGATGTCTT CCACTTTGCT
AATGACTCCA GAAACATGAT ATACATCACC TGCCACCTGA AGGTCACCCT AGCTGAGCAG
GACCCAGATG AACTCAACAA GGCCTGTTCC TTCAGCAAGC CTTCCAACAG CTGGTTCCCA
GTGGAAGGCT CGGCTGACAT CTGTCAATGC TGTAACAAAG GTGACTGTGG CACTCCAAGC
CATTCCAGGA GGCAGCCTCA TGTCATGAGC CAGTGGTCCA GGTCTGCTTC CCGTAACCGC
AGGCATGTGA CAGAAGAAGC AGATGTCACC GTGGGGCCAC TGATCTTCCT GGACAGGAGG
GGTGACCATG AAGTAGAGCA GTGGGCTTTG CCTTCTGACA CCTCAGTGGT GCTGCTGGGC
GTAGGCCTGG CTGTGGTGGT GTCCCTGACT CTGACTGCTG TTATCCTGGT TCTCACCAGG
AGGTGTCGCA CTGCCTCCCA CCCTGTGTCT GCTTCCGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu31830
Clone ID Related Accession (Same CDS sequence) NM_001110354
Accession Version NM_001110354.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product zona pellucida sperm-binding protein 3 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M60504.1, DB328313.1 and BC113949.1. On Nov 17, 2007 this sequence version replaced gi:113419147. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M60504.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAGCTGA GCTATAGGCT CTTCATCTGC CTCCTGCTCT GGGGTAGTAC TGAGCTGTGC 
TACCCCCAAC CCCTCTGGCT CTTGCAGGGT GGAGCCAGCC ATCCTGAGAC GTCCGTACAG
CCCGTACTGG TGGAGTGTCA GGAGGCCACT CTGATGGTCA TGGTCAGCAA AGACCTTTTT
GGCACCGGGA AGCTCATCAG GGCTGCTGAC CTCACCTTGG GCCCAGAGGC CTGTGAGCCT
CTGGTCTCCA TGGACACAGA AGATGTGGTC AGGTTTGAGG TTGGACTCCA CGAGTGTGGC
AACAGCATGC AGGTAACTGA CGATGCCCTG GTGTACAGCA CCTTCCTGCT CCATGACCCC
CGCCCCGTGG GAAACCTGTC CATCGTGAGG ACTAACCGCG CAGAGATTCC CATCGAGTGC
CGCTACCCCA GGCAGGGCAA TGTGAGCAGC CAGGCCATCC TGCCCACCTG GTTGCCCTTC
AGGACCACGG TGTTCTCAGA GGAGAAGCTG ACTTTCTCTC TGCGTCTGAT GGAGGAGAAC
TGGAACGCTG AGAAGAGGTC CCCCACCTTC CACCTGGGAG ATGCAGCCCA CCTCCAGGCA
GAAATCCACA CTGGCAGCCA CGTGCCACTG CGGTTGTTTG TGGACCACTG CGTGGCCACA
CCGACACCAG ACCAGAATGC CTCCCCTTAT CACACCATCG TGGACTTCCA TGGCTGTCTT
GTCGACGGTC TCACTGATGC CTCTTCTGCA TTCAAAGTTC CTCGACCCGG GCCAGATACA
CTCCAGTTCA CAGTGGATGT CTTCCACTTT GCTAATGACT CCAGAAACAT GATATACATC
ACCTGCCACC TGAAGGTCAC CCTAGCTGAG CAGGACCCAG ATGAACTCAA CAAGGCCTGT
TCCTTCAGCA AGCCTTCCAA CAGCTGGTTC CCAGTGGAAG GCTCGGCTGA CATCTGTCAA
TGCTGTAACA AAGGTGACTG TGGCACTCCA AGCCATTCCA GGAGGCAGCC TCATGTCATG
AGCCAGTGGT CCAGGTCTGC TTCCCGTAAC CGCAGGCATG TGACAGAAGA AGCAGATGTC
ACCGTGGGGC CACTGATCTT CCTGGACAGG AGGGGTGACC ATGAAGTAGA GCAGTGGGCT
TTGCCTTCTG ACACCTCAGT GGTGCTGCTG GGCGTAGGCC TGGCTGTGGT GGTGTCCCTG
ACTCTGACTG CTGTTATCCT GGTTCTCACC AGGAGGTGTC GCACTGCCTC CCACCCTGTG
TCTGCTTCCG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001103824.1
CDS11..1285
Misc Feature(1)77..79(+)
Misc Feature(2)143..919(+)
Misc Feature(3)1172..1234(+)
Exon (1)1..322
Gene:ZP3
Gene Synonym:
Exon (2)323..441
Gene:ZP3
Gene Synonym:
Exon (3)442..545
Gene:ZP3
Gene Synonym:
Exon (4)546..723
Gene:ZP3
Gene Synonym:
Exon (5)724..841
Gene:ZP3
Gene Synonym:
Exon (6)842..933
Gene:ZP3
Gene Synonym:
Exon (7)934..1070
Gene:ZP3
Gene Synonym:
Exon (8)1071..1300
Gene:ZP3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001110354
Organism Homo sapiens (human)
Definition Homo sapiens zona pellucida glycoprotein 3 (sperm receptor) (ZP3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001110354

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAGCTGA GCTATAGGCT CTTCATCTGC CTCCTGCTCT GGGGTAGTAC TGAGCTGTGC 
TACCCCCAAC CCCTCTGGCT CTTGCAGGGT GGAGCCAGCC ATCCTGAGAC GTCCGTACAG
CCCGTACTGG TGGAGTGTCA GGAGGCCACT CTGATGGTCA TGGTCAGCAA AGACCTTTTT
GGCACCGGGA AGCTCATCAG GGCTGCTGAC CTCACCTTGG GCCCAGAGGC CTGTGAGCCT
CTGGTCTCCA TGGACACAGA AGATGTGGTC AGGTTTGAGG TTGGACTCCA CGAGTGTGGC
AACAGCATGC AGGTAACTGA CGATGCCCTG GTGTACAGCA CCTTCCTGCT CCATGACCCC
CGCCCCGTGG GAAACCTGTC CATCGTGAGG ACTAACCGCG CAGAGATTCC CATCGAGTGC
CGCTACCCCA GGCAGGGCAA TGTGAGCAGC CAGGCCATCC TGCCCACCTG GTTGCCCTTC
AGGACCACGG TGTTCTCAGA GGAGAAGCTG ACTTTCTCTC TGCGTCTGAT GGAGGAGAAC
TGGAACGCTG AGAAGAGGTC CCCCACCTTC CACCTGGGAG ATGCAGCCCA CCTCCAGGCA
GAAATCCACA CTGGCAGCCA CGTGCCACTG CGGTTGTTTG TGGACCACTG CGTGGCCACA
CCGACACCAG ACCAGAATGC CTCCCCTTAT CACACCATCG TGGACTTCCA TGGCTGTCTT
GTCGACGGTC TCACTGATGC CTCTTCTGCA TTCAAAGTTC CTCGACCCGG GCCAGATACA
CTCCAGTTCA CAGTGGATGT CTTCCACTTT GCTAATGACT CCAGAAACAT GATATACATC
ACCTGCCACC TGAAGGTCAC CCTAGCTGAG CAGGACCCAG ATGAACTCAA CAAGGCCTGT
TCCTTCAGCA AGCCTTCCAA CAGCTGGTTC CCAGTGGAAG GCTCGGCTGA CATCTGTCAA
TGCTGTAACA AAGGTGACTG TGGCACTCCA AGCCATTCCA GGAGGCAGCC TCATGTCATG
AGCCAGTGGT CCAGGTCTGC TTCCCGTAAC CGCAGGCATG TGACAGAAGA AGCAGATGTC
ACCGTGGGGC CACTGATCTT CCTGGACAGG AGGGGTGACC ATGAAGTAGA GCAGTGGGCT
TTGCCTTCTG ACACCTCAGT GGTGCTGCTG GGCGTAGGCC TGGCTGTGGT GGTGTCCCTG
ACTCTGACTG CTGTTATCCT GGTTCTCACC AGGAGGTGTC GCACTGCCTC CCACCCTGTG
TCTGCTTCCG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Genetic and physiological study of morphologically abnormal human zona pellucida
Eur. J. Obstet. Gynecol. Reprod. Biol. 165 (1), 70-76 (2012)
Margalit M, Paz G, Yavetz H, Yogev L, Amit A, Hevlin-Schwartz T, Gupta SK and Kleiman SE.


book

New genetic associations detected in a host response study to hepatitis B vaccine
Genes Immun. 11 (3), 232-238 (2010)
Davila S, Froeling FE, Tan A, Bonnard C, Boland GJ, Snippe H, Hibberd ML and Seielstad M.


book

Identification of human sperm proteins that interact with human zona pellucida3 (ZP3) using yeast two-hybrid system
J. Reprod. Immunol. 84 (1), 24-31 (2010)
Naz RK and Dhandapani L.


book

Binding characteristics of sperm with recombinant human zona pellucida glycoprotein-3 coated beads
Indian J. Med. Res. 130 (1), 37-43 (2009)
Bansal P and Gupta SK.


book

Functional activity of human ZP3 primary sperm receptor resides toward its C-terminus
Biol. Reprod. 81 (1), 7-15 (2009)
Bansal P, Chakrabarti K and Gupta SK.


book

POM-ZP3, a bipartite transcript derived from human ZP3 and a POM121 homologue
Genomics 25 (2), 354-359 (1995)
Kipersztok S, Osawa GA, Liang LF, Modi WS and Dean J.


book

Sperm require beta-N-acetylglucosaminidase to penetrate through the egg zona pellucida
Development 118 (4), 1279-1289 (1993)
Miller DJ, Gong X and Shur BD.


book

The human gene for the zona pellucida glycoprotein ZP3 and a second polymorphic locus are located on chromosome 7
Cytogenet. Cell Genet. 63 (2), 111-113 (1993)
van Duin M, Polman JE, Suikerbuijk RF, Geurts-van Kessel AH and Olijve W.


book

Cloning and characterization of the human sperm receptor ligand ZP3: evidence for a second polymorphic allele with a different frequency in the Caucasian and Japanese populations
Genomics 14 (4), 1064-1070 (1992)
van Duin M, Polman JE, Verkoelen CC, Bunschoten H, Meyerink JH, Olijve W and Aitken RJ.


book

Human homolog of the mouse sperm receptor
Proc. Natl. Acad. Sci. U.S.A. 87 (16), 6014-6018 (1990)
Chamberlin ME and Dean J.


 
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