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ZP3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol ZP3
Entrez Gene ID 7784
Full Name zona pellucida glycoprotein 3 (sperm receptor)
Synonyms ZP3A, ZP3B, ZPC, Zp-3
General protein information
Preferred Names
zona pellucida sperm-binding protein 3
Names
zona pellucida sperm-binding protein 3
ZP3A/ZP3B
sperm receptor
zona pellucida protein C
zona pellucida glycoprotein 3B
zona pellucida glycoprotein ZP3
zona pellucida glycoprotein 3A (sperm receptor)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q11.23

Summary The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

182889

Disorder Html:





The following ZP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18037 NM_007155 Homo sapiens zona pellucida glycoprotein 3 (sperm receptor) (ZP3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu31830 NM_001110354 Homo sapiens zona pellucida glycoprotein 3 (sperm receptor) (ZP3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu18037
Accession Version NM_007155.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1122bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product zona pellucida sperm-binding protein 3 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292763.1, DB328313.1 and BC113949.1. This sequence is a reference standard in the RefSeqGene project. On Nov 16, 2007 this sequence version replaced gi:38327648. Summary: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) differs in the 5' UTR and 5' CDS compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292763.1, BC113949.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGTCATGG TCAGCAAAGA CCTTTTTGGC ACCGGGAAGC TCATCAGGGC TGCTGACCTC 
ACCTTGGGCC CAGAGGCCTG TGAGCCTCTG GTCTCCATGG ACACAGAAGA TGTGGTCAGG
TTTGAGGTTG GACTCCACGA GTGTGGCAAC AGCATGCAGG TAACTGACGA TGCCCTGGTG
TACAGCACCT TCCTGCTCCA TGACCCCCGC CCCGTGGGAA ACCTGTCCAT CGTGAGGACT
AACCGCGCAG AGATTCCCAT CGAGTGCCGC TACCCCAGGC AGGGCAATGT GAGCAGCCAG
GCCATCCTGC CCACCTGGTT GCCCTTCAGG ACCACGGTGT TCTCAGAGGA GAAGCTGACT
TTCTCTCTGC GTCTGATGGA GGAGAACTGG AACGCTGAGA AGAGGTCCCC CACCTTCCAC
CTGGGAGATG CAGCCCACCT CCAGGCAGAA ATCCACACTG GCAGCCACGT GCCACTGCGG
TTGTTTGTGG ACCACTGCGT GGCCACACCG ACACCAGACC AGAATGCCTC CCCTTATCAC
ACCATCGTGG ACTTCCATGG CTGTCTTGTC GACGGTCTCA CTGATGCCTC TTCTGCATTC
AAAGTTCCTC GACCCGGGCC AGATACACTC CAGTTCACAG TGGATGTCTT CCACTTTGCT
AATGACTCCA GAAACATGAT ATACATCACC TGCCACCTGA AGGTCACCCT AGCTGAGCAG
GACCCAGATG AACTCAACAA GGCCTGTTCC TTCAGCAAGC CTTCCAACAG CTGGTTCCCA
GTGGAAGGCT CGGCTGACAT CTGTCAATGC TGTAACAAAG GTGACTGTGG CACTCCAAGC
CATTCCAGGA GGCAGCCTCA TGTCATGAGC CAGTGGTCCA GGTCTGCTTC CCGTAACCGC
AGGCATGTGA CAGAAGAAGC AGATGTCACC GTGGGGCCAC TGATCTTCCT GGACAGGAGG
GGTGACCATG AAGTAGAGCA GTGGGCTTTG CCTTCTGACA CCTCAGTGGT GCTGCTGGGC
GTAGGCCTGG CTGTGGTGGT GTCCCTGACT CTGACTGCTG TTATCCTGGT TCTCACCAGG
AGGTGTCGCA CTGCCTCCCA CCCTGTGTCT GCTTCCGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_009086.4
CDS341..1462
Misc Feature(1)344..1096(+)
Misc Feature(2)1241..1243(+)
Exon (1)1..274
Gene:ZP3
Gene Synonym:
Exon (2)275..499
Gene:ZP3
Gene Synonym:
Exon (3)500..618
Gene:ZP3
Gene Synonym:
Exon (4)619..722
Gene:ZP3
Gene Synonym:
Exon (5)723..900
Gene:ZP3
Gene Synonym:
Exon (6)901..1018
Gene:ZP3
Gene Synonym:
Exon (7)1019..1110
Gene:ZP3
Gene Synonym:
Exon (8)1111..1247
Gene:ZP3
Gene Synonym:
Exon (9)1248..1477
Gene:ZP3
Gene Synonym:
Translation
Position Chain Variation Link
5 5 c, g, t dbSNP:760105811
8 8 g, t dbSNP:752811372
15 15 g, t dbSNP:756213308
19 19 c, g dbSNP:777855682
20 20 a, c, g dbSNP:754019528
28 28 c, g dbSNP:779778771
30 30 c, t dbSNP:746785580
34 34 c, t dbSNP:768764493
39 39 -, caccc dbSNP:765171596
44 44 a, c, g dbSNP:367973598
47 47 g, t dbSNP:769209494
52 52 -, g dbSNP:752179015
60 60 c, t dbSNP:551919386
67 67 c, t dbSNP:762520881
70 70 a, g dbSNP:770616035
71 71 c, g dbSNP:774638389
79 79 a, c, t dbSNP:566830363
80 80 a, c, g dbSNP:139299212
83 83 c, g dbSNP:761109308
91 91 a, g dbSNP:764085193
96 96 a, c dbSNP:201010357
97 97 c, t dbSNP:757478111
106 106 a, g dbSNP:77008819
110 110 g, t dbSNP:751442426
116 116 c, t dbSNP:754853681
122 122 a, g dbSNP:781086528
123 123 a, c dbSNP:748110203
130 130 c, t dbSNP:756017367
131 131 a, g dbSNP:199654774
133 133 a, g dbSNP:748821832
138 138 g, t dbSNP:770541886
140 140 g, t dbSNP:774020638
141 141 c, t dbSNP:745470047
144 144 g, t dbSNP:772435658
156 156 a, g dbSNP:775764139
162 162 a, g dbSNP:537628334
163 163 c, t dbSNP:764647298
167 167 c, g dbSNP:776622196
172 172 c, g dbSNP:761923569
173 173 c, t dbSNP:765193217
185 185 a, g dbSNP:750697733
187 187 a, c, g dbSNP:375655664
191 191 a, g dbSNP:752540133
192 192 a, c, g dbSNP:756066961
194 194 a, g dbSNP:749136887
200 200 c, t dbSNP:756811945
206 206 c, t dbSNP:778331965
210 210 c, t dbSNP:745520133
245 245 a, g dbSNP:771706820
263 263 -, gccc dbSNP:755592766
269 269 -, aca dbSNP:779470385
269 269 a, c dbSNP:780454436
276 276 a, g dbSNP:756687886
278 278 a, g dbSNP:2286428
279 279 a, g dbSNP:749309704
281 281 a, g dbSNP:779345366
287 287 c, t dbSNP:770998544
288 288 a, g dbSNP:774455291
295 295 c, g dbSNP:117869702
297 297 c, t dbSNP:768441500
298 298 a, g dbSNP:758754773
301 301 c, t dbSNP:199647928
302 302 a, g dbSNP:370377360
310 310 c, t dbSNP:114691990
311 311 a, g dbSNP:552362407
316 316 c, g dbSNP:765865017
321 321 a, g dbSNP:751186472
325 325 g, t dbSNP:759200816
327 327 a, g dbSNP:768063875
331 331 c, g dbSNP:753102108
333 333 c, g dbSNP:570702038
336 336 a, c, t dbSNP:778451723
341 341 a, t dbSNP:757295183
342 342 g, t dbSNP:778982670
343 343 a, g dbSNP:746141666
347 347 a, g dbSNP:772266862
349 349 a, g dbSNP:781012666
358 358 a, g dbSNP:534473985
360 360 a, g dbSNP:747906357
361 361 c, t dbSNP:769537682
366 366 c, t dbSNP:773046518
370 370 c, t dbSNP:762892301
373 373 c, t dbSNP:115350845
374 374 a, g dbSNP:773910150
376 376 a, g dbSNP:552823307
382 382 c, t dbSNP:568030181
385 385 c, t dbSNP:142032111
388 388 a, g dbSNP:369220809
389 389 g, t dbSNP:764458859
391 391 c, t dbSNP:754303319
393 393 c, t dbSNP:778344978
397 397 c, t dbSNP:535069290
398 398 c, t dbSNP:750478339
402 402 a, c dbSNP:758658851
403 403 c, g dbSNP:780286641
409 409 c, t dbSNP:143341902
416 416 a, g dbSNP:769581162
419 419 -, tgtgagcct dbSNP:748349393
425 425 a, c dbSNP:202083521
428 428 -, a dbSNP:770900728
428 428 c, g dbSNP:749239918
429 429 c, t dbSNP:151289035
431 431 a, g dbSNP:773770548
432 432 a, c, t dbSNP:745345934
433 433 c, t dbSNP:775289486
435 435 c, t dbSNP:377192244
436 436 c, t dbSNP:760349973
438 438 c, t dbSNP:574417097
443 443 a, g dbSNP:764426889
446 446 a, g dbSNP:777051289
449 449 g, t dbSNP:140647241
453 453 g, t dbSNP:765752672
467 467 a, g dbSNP:150482826
468 468 c, t dbSNP:758504443
478 478 c, t dbSNP:142370942
479 479 a, g dbSNP:751792813
480 480 a, c dbSNP:755212923
485 485 g, t dbSNP:777493256
490 490 c, t dbSNP:749076742
492 492 a, g dbSNP:757187128
493 493 -, a dbSNP:776588795
493 493 c, t dbSNP:778927660
496 496 g, t dbSNP:745737938
498 498 a, g, t dbSNP:747489932
499 499 c, g dbSNP:775129366
508 508 c, t dbSNP:750215338
509 509 a, c, g dbSNP:144871193
520 520 a, g dbSNP:142750355
523 523 c, t dbSNP:375567174
525 525 a, g dbSNP:140906109
534 534 c, t dbSNP:780510553
535 535 a, g dbSNP:747891597
541 541 c, t dbSNP:769431095
548 548 a, c dbSNP:778149636
549 549 a, g dbSNP:368564987
550 550 c, t dbSNP:182977240
553 553 c, t dbSNP:774866065
554 554 a, g dbSNP:371803648
556 556 a, g dbSNP:772255462
566 566 c, t dbSNP:775430011
568 568 c, t dbSNP:144953873
569 569 a, g dbSNP:373571562
571 571 a, c, t dbSNP:754066676
572 572 a, g dbSNP:144669807
584 584 c, g, t dbSNP:149364780
585 585 a, g, t dbSNP:138711194
586 586 c, t dbSNP:755810089
589 589 -, ag dbSNP:765091119
596 596 c, g, t dbSNP:777422281
604 604 c, g dbSNP:757618822
608 608 a, c, t dbSNP:777581823
609 609 a, g, t dbSNP:772430243
619 619 a, g, t dbSNP:199644652
620 620 c, t dbSNP:781693114
628 628 c, g, t dbSNP:377382354
636 636 a, g dbSNP:763664408
672 672 c, t dbSNP:200478046
675 675 c, t dbSNP:761504196
676 676 a, g dbSNP:764822912
679 679 a, g dbSNP:572129555
683 683 g, t dbSNP:144048482
693 693 a, c dbSNP:199926330
694 694 a, g dbSNP:201006719
710 710 a, c, g, t dbSNP:372042157
711 711 a, c, g dbSNP:200472178
712 712 a, t dbSNP:769861881
717 717 -, g dbSNP:751250796
717 717 c, t dbSNP:777885841
724 724 g, t dbSNP:776556367
733 733 a, c, t dbSNP:371699247
734 734 a, g dbSNP:74676082
742 742 c, g dbSNP:772775993
744 744 a, g dbSNP:762551776
748 748 a, c, t dbSNP:766107926
749 749 c, t dbSNP:760008906
754 754 c, g dbSNP:767924518
757 757 c, g dbSNP:753155780
763 763 a, g dbSNP:375864419
764 764 a, g dbSNP:764124207
767 767 a, c, g dbSNP:753936084
770 770 a, g dbSNP:141376418
771 771 c, t dbSNP:746023959
773 773 g, t dbSNP:754783232
774 774 c, t dbSNP:780951371
775 775 c, t dbSNP:748101510
776 776 c, t dbSNP:769739244
795 795 a, g dbSNP:772646403
800 800 a, g dbSNP:748850636
808 808 c, g, t dbSNP:371263995
809 809 a, g dbSNP:759204850
810 810 c, t dbSNP:768047547
811 811 a, g dbSNP:775915687
812 812 a, c dbSNP:772852039
814 814 a, g dbSNP:761139076
816 816 c, t dbSNP:764688890
818 818 c, t dbSNP:754262487
819 819 a, g dbSNP:757336467
820 820 a, g dbSNP:765244181
826 826 g, t dbSNP:750702425
832 832 c, t dbSNP:758617633
838 838 c, t dbSNP:373009553
839 839 a, g dbSNP:374513771
845 845 a, g dbSNP:755814911
848 848 c, g dbSNP:777796401
849 849 c, g, t dbSNP:139729790
850 850 a, g dbSNP:367627395
853 853 a, g dbSNP:745580577
854 854 a, c dbSNP:771816389
868 868 a, c dbSNP:772983332
870 870 c, t dbSNP:761136725
872 872 c, t dbSNP:768839548
886 886 c, t dbSNP:777208815
887 887 a, g dbSNP:762295444
893 893 c, t dbSNP:145049890
897 897 a, g dbSNP:562729976
898 898 c, t dbSNP:199660958
907 907 c, t dbSNP:753652463
910 910 c, g, t dbSNP:112208102
913 913 c, t dbSNP:750323765
914 914 a, g dbSNP:147549399
944 944 g, t dbSNP:536596725
947 947 c, g dbSNP:746743218
948 948 c, g dbSNP:768164800
955 955 c, t dbSNP:781586493
956 956 a, g dbSNP:748534079
964 964 c, t dbSNP:770102119
970 970 c, g dbSNP:372269444
985 985 c, t dbSNP:558532708
1002 1002 a, g dbSNP:773712433
1019 1019 a, g dbSNP:775871630
1022 1022 c, t dbSNP:760969466
1024 1024 c, t dbSNP:372475962
1030 1030 c, g, t dbSNP:773111220
1034 1034 c, t dbSNP:149689401
1040 1040 a, g dbSNP:751472208
1048 1048 a, c dbSNP:754448713
1053 1053 c, g dbSNP:766955002
1062 1062 a, t dbSNP:752404438
1065 1065 c, g dbSNP:145839945
1066 1066 a, c dbSNP:778145941
1070 1070 -, g dbSNP:755052834
1081 1081 a, g dbSNP:73363163
1082 1082 g, t dbSNP:757589719
1087 1087 c, t dbSNP:779295502
1089 1089 -, c dbSNP:779116087
1095 1095 a, g dbSNP:746330441
1099 1099 g, t dbSNP:772339909
1101 1101 c, t dbSNP:780281013
1122 1122 c, g, t dbSNP:778618304
1123 1123 a, g dbSNP:771810947
1129 1129 a, c dbSNP:148976073
1130 1130 c, t dbSNP:2906999
1131 1131 c, t dbSNP:768121709
1132 1132 a, g dbSNP:553842143
1134 1134 c, g dbSNP:761425411
1142 1142 a, t dbSNP:764966389
1147 1147 a, g dbSNP:372434766
1150 1150 c, t dbSNP:772960303
1152 1152 a, g dbSNP:763416309
1153 1153 g, t dbSNP:766790658
1158 1158 a, g dbSNP:752168165
1160 1160 a, g dbSNP:146640456
1170 1170 g, t dbSNP:767534569
1175 1175 c, t dbSNP:191720772
1176 1176 c, t dbSNP:756094155
1181 1181 c, t dbSNP:371583399
1188 1188 -, g dbSNP:775352304
1195 1195 a, g dbSNP:749442543
1200 1200 a, g dbSNP:2906998
1202 1202 a, g dbSNP:779822538
1204 1204 c, t dbSNP:746992574
1205 1205 a, g dbSNP:2906997
1206 1206 g, t dbSNP:776383800
1210 1210 a, c dbSNP:374402754
1211 1211 c, t dbSNP:769119680
1216 1216 g, t dbSNP:773015077
1221 1221 c, g dbSNP:1804905
1222 1222 a, g dbSNP:766916429
1225 1225 g, t dbSNP:141493396
1227 1227 c, t dbSNP:760009534
1228 1228 c, t dbSNP:768086870
1230 1230 c, t dbSNP:753100841
1233 1233 a, g dbSNP:756220215
1238 1238 a, c, t dbSNP:764100090
1239 1239 a, c, g, t dbSNP:377699467
1240 1240 c, t dbSNP:754689780
1245 1245 a, g dbSNP:531739153
1269 1269 c, t dbSNP:201448034
1270 1270 c, t dbSNP:201735348
1271 1271 a, g dbSNP:765246190
1272 1272 -, g dbSNP:774054183
1272 1272 c, t dbSNP:750717514
1280 1280 a, c dbSNP:763079407
1285 1285 a, c dbSNP:767383001
1292 1292 g, t dbSNP:752409580
1297 1297 -, t dbSNP:761250308
1300 1300 g, t dbSNP:200481427
1301 1301 a, g dbSNP:764158147
1306 1306 -, c dbSNP:767173340
1308 1308 a, g dbSNP:753760903
1317 1317 a, g dbSNP:143649134
1318 1318 a, g dbSNP:778271151
1322 1322 c, t dbSNP:745576248
1326 1326 c, t dbSNP:201521728
1332 1332 c, t dbSNP:779709002
1354 1354 c, g dbSNP:747365569
1357 1357 c, g dbSNP:768946806
1360 1360 c, t dbSNP:777139655
1361 1361 a, g dbSNP:201251126
1367 1367 c, t dbSNP:769930483
1382 1382 c, t dbSNP:773132208
1384 1384 c, t dbSNP:763280959
1385 1385 c, g, t dbSNP:766528260
1386 1386 c, t dbSNP:760550498
1389 1389 -, ct dbSNP:750081136
1390 1390 a, t dbSNP:763923046
1402 1402 c, t dbSNP:753883780
1403 1403 a, g dbSNP:757253675
1404 1404 a, t dbSNP:764853903
1408 1408 c, g dbSNP:749924852
1412 1412 c, t dbSNP:757886196
1420 1420 a, g dbSNP:538143531
1440 1440 a, g dbSNP:201964956
1457 1457 a, g dbSNP:755397483
1463 1463 -, aag dbSNP:761016268
1472 1472 g, t dbSNP:781535325

Target ORF information:

RefSeq Version NM_007155
Organism Homo sapiens (human)
Definition Homo sapiens zona pellucida glycoprotein 3 (sperm receptor) (ZP3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007155

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGTCATGG TCAGCAAAGA CCTTTTTGGC ACCGGGAAGC TCATCAGGGC TGCTGACCTC 
ACCTTGGGCC CAGAGGCCTG TGAGCCTCTG GTCTCCATGG ACACAGAAGA TGTGGTCAGG
TTTGAGGTTG GACTCCACGA GTGTGGCAAC AGCATGCAGG TAACTGACGA TGCCCTGGTG
TACAGCACCT TCCTGCTCCA TGACCCCCGC CCCGTGGGAA ACCTGTCCAT CGTGAGGACT
AACCGCGCAG AGATTCCCAT CGAGTGCCGC TACCCCAGGC AGGGCAATGT GAGCAGCCAG
GCCATCCTGC CCACCTGGTT GCCCTTCAGG ACCACGGTGT TCTCAGAGGA GAAGCTGACT
TTCTCTCTGC GTCTGATGGA GGAGAACTGG AACGCTGAGA AGAGGTCCCC CACCTTCCAC
CTGGGAGATG CAGCCCACCT CCAGGCAGAA ATCCACACTG GCAGCCACGT GCCACTGCGG
TTGTTTGTGG ACCACTGCGT GGCCACACCG ACACCAGACC AGAATGCCTC CCCTTATCAC
ACCATCGTGG ACTTCCATGG CTGTCTTGTC GACGGTCTCA CTGATGCCTC TTCTGCATTC
AAAGTTCCTC GACCCGGGCC AGATACACTC CAGTTCACAG TGGATGTCTT CCACTTTGCT
AATGACTCCA GAAACATGAT ATACATCACC TGCCACCTGA AGGTCACCCT AGCTGAGCAG
GACCCAGATG AACTCAACAA GGCCTGTTCC TTCAGCAAGC CTTCCAACAG CTGGTTCCCA
GTGGAAGGCT CGGCTGACAT CTGTCAATGC TGTAACAAAG GTGACTGTGG CACTCCAAGC
CATTCCAGGA GGCAGCCTCA TGTCATGAGC CAGTGGTCCA GGTCTGCTTC CCGTAACCGC
AGGCATGTGA CAGAAGAAGC AGATGTCACC GTGGGGCCAC TGATCTTCCT GGACAGGAGG
GGTGACCATG AAGTAGAGCA GTGGGCTTTG CCTTCTGACA CCTCAGTGGT GCTGCTGGGC
GTAGGCCTGG CTGTGGTGGT GTCCCTGACT CTGACTGCTG TTATCCTGGT TCTCACCAGG
AGGTGTCGCA CTGCCTCCCA CCCTGTGTCT GCTTCCGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu31830
Accession Version NM_001110354.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product zona pellucida sperm-binding protein 3 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M60504.1, DB328313.1 and BC113949.1. On Nov 17, 2007 this sequence version replaced gi:113419147. Summary: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M60504.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAGCTGA GCTATAGGCT CTTCATCTGC CTCCTGCTCT GGGGTAGTAC TGAGCTGTGC 
TACCCCCAAC CCCTCTGGCT CTTGCAGGGT GGAGCCAGCC ATCCTGAGAC GTCCGTACAG
CCCGTACTGG TGGAGTGTCA GGAGGCCACT CTGATGGTCA TGGTCAGCAA AGACCTTTTT
GGCACCGGGA AGCTCATCAG GGCTGCTGAC CTCACCTTGG GCCCAGAGGC CTGTGAGCCT
CTGGTCTCCA TGGACACAGA AGATGTGGTC AGGTTTGAGG TTGGACTCCA CGAGTGTGGC
AACAGCATGC AGGTAACTGA CGATGCCCTG GTGTACAGCA CCTTCCTGCT CCATGACCCC
CGCCCCGTGG GAAACCTGTC CATCGTGAGG ACTAACCGCG CAGAGATTCC CATCGAGTGC
CGCTACCCCA GGCAGGGCAA TGTGAGCAGC CAGGCCATCC TGCCCACCTG GTTGCCCTTC
AGGACCACGG TGTTCTCAGA GGAGAAGCTG ACTTTCTCTC TGCGTCTGAT GGAGGAGAAC
TGGAACGCTG AGAAGAGGTC CCCCACCTTC CACCTGGGAG ATGCAGCCCA CCTCCAGGCA
GAAATCCACA CTGGCAGCCA CGTGCCACTG CGGTTGTTTG TGGACCACTG CGTGGCCACA
CCGACACCAG ACCAGAATGC CTCCCCTTAT CACACCATCG TGGACTTCCA TGGCTGTCTT
GTCGACGGTC TCACTGATGC CTCTTCTGCA TTCAAAGTTC CTCGACCCGG GCCAGATACA
CTCCAGTTCA CAGTGGATGT CTTCCACTTT GCTAATGACT CCAGAAACAT GATATACATC
ACCTGCCACC TGAAGGTCAC CCTAGCTGAG CAGGACCCAG ATGAACTCAA CAAGGCCTGT
TCCTTCAGCA AGCCTTCCAA CAGCTGGTTC CCAGTGGAAG GCTCGGCTGA CATCTGTCAA
TGCTGTAACA AAGGTGACTG TGGCACTCCA AGCCATTCCA GGAGGCAGCC TCATGTCATG
AGCCAGTGGT CCAGGTCTGC TTCCCGTAAC CGCAGGCATG TGACAGAAGA AGCAGATGTC
ACCGTGGGGC CACTGATCTT CCTGGACAGG AGGGGTGACC ATGAAGTAGA GCAGTGGGCT
TTGCCTTCTG ACACCTCAGT GGTGCTGCTG GGCGTAGGCC TGGCTGTGGT GGTGTCCCTG
ACTCTGACTG CTGTTATCCT GGTTCTCACC AGGAGGTGTC GCACTGCCTC CCACCCTGTG
TCTGCTTCCG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001103824.1
CDS11..1285
Misc Feature(1)77..79(+)
Misc Feature(2)143..919(+)
Misc Feature(3)1172..1234(+)
Exon (1)1..322
Gene:ZP3
Gene Synonym:
Exon (2)323..441
Gene:ZP3
Gene Synonym:
Exon (3)442..545
Gene:ZP3
Gene Synonym:
Exon (4)546..723
Gene:ZP3
Gene Synonym:
Exon (5)724..841
Gene:ZP3
Gene Synonym:
Exon (6)842..933
Gene:ZP3
Gene Synonym:
Exon (7)934..1070
Gene:ZP3
Gene Synonym:
Exon (8)1071..1300
Gene:ZP3
Gene Synonym:
Translation
Position Chain Variation Link
1 1 g, t dbSNP:775147186
8 8 a, t dbSNP:760727004
9 9 c, t dbSNP:111488442
14 14 a, g dbSNP:753731255
15 15 a, g, t dbSNP:756778893
19 19 a, g dbSNP:750078193
23 23 g, t dbSNP:757981555
29 29 c, g dbSNP:780525296
31 31 c, g, t dbSNP:373904061
39 39 a, g dbSNP:754133352
41 41 c, t dbSNP:781772760
43 43 c, g, t dbSNP:148715886
53 53 a, g dbSNP:541167188
54 54 a, g, t dbSNP:749594370
60 60 c, t dbSNP:775390674
64 64 -, g dbSNP:774781857
65 65 a, c dbSNP:760414285
68 68 c, t dbSNP:763824181
74 74 a, c, g dbSNP:776560621
76 76 c, g, t dbSNP:764793375
80 80 c, t dbSNP:758119087
89 89 c, t dbSNP:765917789
94 94 a, g, t dbSNP:751185738
95 95 c, t dbSNP:781643945
97 97 g, t dbSNP:748672253
99 99 a, g dbSNP:756687886
101 101 a, g dbSNP:2286428
102 102 a, g dbSNP:749309704
104 104 a, g dbSNP:779345366
110 110 c, t dbSNP:770998544
111 111 a, g dbSNP:774455291
118 118 c, g dbSNP:117869702
120 120 c, t dbSNP:768441500
121 121 a, g dbSNP:758754773
124 124 c, t dbSNP:199647928
125 125 a, g dbSNP:370377360
133 133 c, t dbSNP:114691990
134 134 a, g dbSNP:552362407
139 139 c, g dbSNP:765865017
144 144 a, g dbSNP:751186472
148 148 g, t dbSNP:759200816
150 150 a, g dbSNP:768063875
154 154 c, g dbSNP:753102108
156 156 c, g dbSNP:570702038
159 159 a, c, t dbSNP:778451723
164 164 a, t dbSNP:757295183
165 165 g, t dbSNP:778982670
166 166 a, g dbSNP:746141666
170 170 a, g dbSNP:772266862
172 172 a, g dbSNP:781012666
181 181 a, g dbSNP:534473985
183 183 a, g dbSNP:747906357
184 184 c, t dbSNP:769537682
189 189 c, t dbSNP:773046518
193 193 c, t dbSNP:762892301
196 196 c, t dbSNP:115350845
197 197 a, g dbSNP:773910150
199 199 a, g dbSNP:552823307
205 205 c, t dbSNP:568030181
208 208 c, t dbSNP:142032111
211 211 a, g dbSNP:369220809
212 212 g, t dbSNP:764458859
214 214 c, t dbSNP:754303319
216 216 c, t dbSNP:778344978
220 220 c, t dbSNP:535069290
221 221 c, t dbSNP:750478339
225 225 a, c dbSNP:758658851
226 226 c, g dbSNP:780286641
232 232 c, t dbSNP:143341902
239 239 a, g dbSNP:769581162
242 242 -, tgtgagcct dbSNP:748349393
248 248 a, c dbSNP:202083521
251 251 -, a dbSNP:770900728
251 251 c, g dbSNP:749239918
252 252 c, t dbSNP:151289035
254 254 a, g dbSNP:773770548
255 255 a, c, t dbSNP:745345934
256 256 c, t dbSNP:775289486
258 258 c, t dbSNP:377192244
259 259 c, t dbSNP:760349973
261 261 c, t dbSNP:574417097
266 266 a, g dbSNP:764426889
269 269 a, g dbSNP:777051289
272 272 g, t dbSNP:140647241
276 276 g, t dbSNP:765752672
290 290 a, g dbSNP:150482826
291 291 c, t dbSNP:758504443
301 301 c, t dbSNP:142370942
302 302 a, g dbSNP:751792813
303 303 a, c dbSNP:755212923
308 308 g, t dbSNP:777493256
313 313 c, t dbSNP:749076742
315 315 a, g dbSNP:757187128
316 316 -, a dbSNP:776588795
316 316 c, t dbSNP:778927660
319 319 g, t dbSNP:745737938
321 321 a, g, t dbSNP:747489932
322 322 c, g dbSNP:775129366
331 331 c, t dbSNP:750215338
332 332 a, c, g dbSNP:144871193
343 343 a, g dbSNP:142750355
346 346 c, t dbSNP:375567174
348 348 a, g dbSNP:140906109
357 357 c, t dbSNP:780510553
358 358 a, g dbSNP:747891597
364 364 c, t dbSNP:769431095
371 371 a, c dbSNP:778149636
372 372 a, g dbSNP:368564987
373 373 c, t dbSNP:182977240
376 376 c, t dbSNP:774866065
377 377 a, g dbSNP:371803648
379 379 a, g dbSNP:772255462
389 389 c, t dbSNP:775430011
391 391 c, t dbSNP:144953873
392 392 a, g dbSNP:373571562
394 394 a, c, t dbSNP:754066676
395 395 a, g dbSNP:144669807
407 407 c, g, t dbSNP:149364780
408 408 a, g, t dbSNP:138711194
409 409 c, t dbSNP:755810089
412 412 -, ag dbSNP:765091119
419 419 c, g, t dbSNP:777422281
427 427 c, g dbSNP:757618822
431 431 a, c, t dbSNP:777581823
432 432 a, g, t dbSNP:772430243
442 442 a, g, t dbSNP:199644652
443 443 c, t dbSNP:781693114
451 451 c, g, t dbSNP:377382354
459 459 a, g dbSNP:763664408
495 495 c, t dbSNP:200478046
498 498 c, t dbSNP:761504196
499 499 a, g dbSNP:764822912
502 502 a, g dbSNP:572129555
506 506 g, t dbSNP:144048482
516 516 a, c dbSNP:199926330
517 517 a, g dbSNP:201006719
533 533 a, c, g, t dbSNP:372042157
534 534 a, c, g dbSNP:200472178
535 535 a, t dbSNP:769861881
540 540 -, g dbSNP:751250796
540 540 c, t dbSNP:777885841
547 547 g, t dbSNP:776556367
556 556 a, c, t dbSNP:371699247
557 557 a, g dbSNP:74676082
565 565 c, g dbSNP:772775993
567 567 a, g dbSNP:762551776
571 571 a, c, t dbSNP:766107926
572 572 c, t dbSNP:760008906
577 577 c, g dbSNP:767924518
580 580 c, g dbSNP:753155780
586 586 a, g dbSNP:375864419
587 587 a, g dbSNP:764124207
590 590 a, c, g dbSNP:753936084
593 593 a, g dbSNP:141376418
594 594 c, t dbSNP:746023959
596 596 g, t dbSNP:754783232
597 597 c, t dbSNP:780951371
598 598 c, t dbSNP:748101510
599 599 c, t dbSNP:769739244
618 618 a, g dbSNP:772646403
623 623 a, g dbSNP:748850636
631 631 c, g, t dbSNP:371263995
632 632 a, g dbSNP:759204850
633 633 c, t dbSNP:768047547
634 634 a, g dbSNP:775915687
635 635 a, c dbSNP:772852039
637 637 a, g dbSNP:761139076
639 639 c, t dbSNP:764688890
641 641 c, t dbSNP:754262487
642 642 a, g dbSNP:757336467
643 643 a, g dbSNP:765244181
649 649 g, t dbSNP:750702425
655 655 c, t dbSNP:758617633
661 661 c, t dbSNP:373009553
662 662 a, g dbSNP:374513771
668 668 a, g dbSNP:755814911
671 671 c, g dbSNP:777796401
672 672 c, g, t dbSNP:139729790
673 673 a, g dbSNP:367627395
676 676 a, g dbSNP:745580577
677 677 a, c dbSNP:771816389
691 691 a, c dbSNP:772983332
693 693 c, t dbSNP:761136725
695 695 c, t dbSNP:768839548
709 709 c, t dbSNP:777208815
710 710 a, g dbSNP:762295444
716 716 c, t dbSNP:145049890
720 720 a, g dbSNP:562729976
721 721 c, t dbSNP:199660958
730 730 c, t dbSNP:753652463
733 733 c, g, t dbSNP:112208102
736 736 c, t dbSNP:750323765
737 737 a, g dbSNP:147549399
767 767 g, t dbSNP:536596725
770 770 c, g dbSNP:746743218
771 771 c, g dbSNP:768164800
778 778 c, t dbSNP:781586493
779 779 a, g dbSNP:748534079
787 787 c, t dbSNP:770102119
793 793 c, g dbSNP:372269444
808 808 c, t dbSNP:558532708
825 825 a, g dbSNP:773712433
842 842 a, g dbSNP:775871630
845 845 c, t dbSNP:760969466
847 847 c, t dbSNP:372475962
853 853 c, g, t dbSNP:773111220
857 857 c, t dbSNP:149689401
863 863 a, g dbSNP:751472208
871 871 a, c dbSNP:754448713
876 876 c, g dbSNP:766955002
885 885 a, t dbSNP:752404438
888 888 c, g dbSNP:145839945
889 889 a, c dbSNP:778145941
893 893 -, g dbSNP:755052834
904 904 a, g dbSNP:73363163
905 905 g, t dbSNP:757589719
910 910 c, t dbSNP:779295502
912 912 -, c dbSNP:779116087
918 918 a, g dbSNP:746330441
922 922 g, t dbSNP:772339909
924 924 c, t dbSNP:780281013
945 945 c, g, t dbSNP:778618304
946 946 a, g dbSNP:771810947
952 952 a, c dbSNP:148976073
953 953 c, t dbSNP:2906999
954 954 c, t dbSNP:768121709
955 955 a, g dbSNP:553842143
957 957 c, g dbSNP:761425411
965 965 a, t dbSNP:764966389
970 970 a, g dbSNP:372434766
973 973 c, t dbSNP:772960303
975 975 a, g dbSNP:763416309
976 976 g, t dbSNP:766790658
981 981 a, g dbSNP:752168165
983 983 a, g dbSNP:146640456
993 993 g, t dbSNP:767534569
998 998 c, t dbSNP:191720772
999 999 c, t dbSNP:756094155
1004 1004 c, t dbSNP:371583399
1011 1011 -, g dbSNP:775352304
1018 1018 a, g dbSNP:749442543
1023 1023 a, g dbSNP:2906998
1025 1025 a, g dbSNP:779822538
1027 1027 c, t dbSNP:746992574
1028 1028 a, g dbSNP:2906997
1029 1029 g, t dbSNP:776383800
1033 1033 a, c dbSNP:374402754
1034 1034 c, t dbSNP:769119680
1039 1039 g, t dbSNP:773015077
1044 1044 c, g dbSNP:1804905
1045 1045 a, g dbSNP:766916429
1048 1048 g, t dbSNP:141493396
1050 1050 c, t dbSNP:760009534
1051 1051 c, t dbSNP:768086870
1053 1053 c, t dbSNP:753100841
1056 1056 a, g dbSNP:756220215
1061 1061 a, c, t dbSNP:764100090
1062 1062 a, c, g, t dbSNP:377699467
1063 1063 c, t dbSNP:754689780
1068 1068 a, g dbSNP:531739153
1092 1092 c, t dbSNP:201448034
1093 1093 c, t dbSNP:201735348
1094 1094 a, g dbSNP:765246190
1095 1095 -, g dbSNP:774054183
1095 1095 c, t dbSNP:750717514
1103 1103 a, c dbSNP:763079407
1108 1108 a, c dbSNP:767383001
1115 1115 g, t dbSNP:752409580
1120 1120 -, t dbSNP:761250308
1123 1123 g, t dbSNP:200481427
1124 1124 a, g dbSNP:764158147
1129 1129 -, c dbSNP:767173340
1131 1131 a, g dbSNP:753760903
1140 1140 a, g dbSNP:143649134
1141 1141 a, g dbSNP:778271151
1145 1145 c, t dbSNP:745576248
1149 1149 c, t dbSNP:201521728
1155 1155 c, t dbSNP:779709002
1177 1177 c, g dbSNP:747365569
1180 1180 c, g dbSNP:768946806
1183 1183 c, t dbSNP:777139655
1184 1184 a, g dbSNP:201251126
1190 1190 c, t dbSNP:769930483
1205 1205 c, t dbSNP:773132208
1207 1207 c, t dbSNP:763280959
1208 1208 c, g, t dbSNP:766528260
1209 1209 c, t dbSNP:760550498
1212 1212 -, ct dbSNP:750081136
1213 1213 a, t dbSNP:763923046
1225 1225 c, t dbSNP:753883780
1226 1226 a, g dbSNP:757253675
1227 1227 a, t dbSNP:764853903
1231 1231 c, g dbSNP:749924852
1235 1235 c, t dbSNP:757886196
1243 1243 a, g dbSNP:538143531
1263 1263 a, g dbSNP:201964956
1280 1280 a, g dbSNP:755397483
1286 1286 -, aag dbSNP:761016268
1295 1295 g, t dbSNP:781535325

Target ORF information:

RefSeq Version NM_001110354
Organism Homo sapiens (human)
Definition Homo sapiens zona pellucida glycoprotein 3 (sperm receptor) (ZP3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001110354

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAGCTGA GCTATAGGCT CTTCATCTGC CTCCTGCTCT GGGGTAGTAC TGAGCTGTGC 
TACCCCCAAC CCCTCTGGCT CTTGCAGGGT GGAGCCAGCC ATCCTGAGAC GTCCGTACAG
CCCGTACTGG TGGAGTGTCA GGAGGCCACT CTGATGGTCA TGGTCAGCAA AGACCTTTTT
GGCACCGGGA AGCTCATCAG GGCTGCTGAC CTCACCTTGG GCCCAGAGGC CTGTGAGCCT
CTGGTCTCCA TGGACACAGA AGATGTGGTC AGGTTTGAGG TTGGACTCCA CGAGTGTGGC
AACAGCATGC AGGTAACTGA CGATGCCCTG GTGTACAGCA CCTTCCTGCT CCATGACCCC
CGCCCCGTGG GAAACCTGTC CATCGTGAGG ACTAACCGCG CAGAGATTCC CATCGAGTGC
CGCTACCCCA GGCAGGGCAA TGTGAGCAGC CAGGCCATCC TGCCCACCTG GTTGCCCTTC
AGGACCACGG TGTTCTCAGA GGAGAAGCTG ACTTTCTCTC TGCGTCTGAT GGAGGAGAAC
TGGAACGCTG AGAAGAGGTC CCCCACCTTC CACCTGGGAG ATGCAGCCCA CCTCCAGGCA
GAAATCCACA CTGGCAGCCA CGTGCCACTG CGGTTGTTTG TGGACCACTG CGTGGCCACA
CCGACACCAG ACCAGAATGC CTCCCCTTAT CACACCATCG TGGACTTCCA TGGCTGTCTT
GTCGACGGTC TCACTGATGC CTCTTCTGCA TTCAAAGTTC CTCGACCCGG GCCAGATACA
CTCCAGTTCA CAGTGGATGT CTTCCACTTT GCTAATGACT CCAGAAACAT GATATACATC
ACCTGCCACC TGAAGGTCAC CCTAGCTGAG CAGGACCCAG ATGAACTCAA CAAGGCCTGT
TCCTTCAGCA AGCCTTCCAA CAGCTGGTTC CCAGTGGAAG GCTCGGCTGA CATCTGTCAA
TGCTGTAACA AAGGTGACTG TGGCACTCCA AGCCATTCCA GGAGGCAGCC TCATGTCATG
AGCCAGTGGT CCAGGTCTGC TTCCCGTAAC CGCAGGCATG TGACAGAAGA AGCAGATGTC
ACCGTGGGGC CACTGATCTT CCTGGACAGG AGGGGTGACC ATGAAGTAGA GCAGTGGGCT
TTGCCTTCTG ACACCTCAGT GGTGCTGCTG GGCGTAGGCC TGGCTGTGGT GGTGTCCCTG
ACTCTGACTG CTGTTATCCT GGTTCTCACC AGGAGGTGTC GCACTGCCTC CCACCCTGTG
TCTGCTTCCG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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