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TUBA1A cDNA ORF clone, Homo sapiens (human)

Gene Symbol TUBA1A
Entrez Gene ID 7846
Full Name tubulin, alpha 1a
Synonyms B-ALPHA-1, LIS3, TUBA3
General protein information
Preferred Names
tubulin alpha-1A chain
Names
tubulin alpha-1A chain
hum-a-tub1
hum-a-tub2
tubulin B-alpha-1
tubulin alpha-3 chain
tubulin, alpha, brain-specific
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q13.12

Summary Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]. lac of sum
Disorder MIM:

602529

Disorder Html: Lissencephaly 3, 611603 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_006009 NP_006000 tubulin alpha-1A chain isoform 1
NM_001270399 NP_001257328 tubulin alpha-1A chain isoform 1
NM_001270400 NP_001257329 tubulin alpha-1A chain isoform 2



Homo sapiens (human) TUBA1A NP_006000.2
Pan troglodytes (chimpanzee) TUBA1A NP_001092014.1
Macaca mulatta (Rhesus monkey) TUBA1A XP_001108924.2
Canis lupus familiaris (dog) TUBA1A XP_862471.2
Bos taurus (cattle) TUBA1A NP_001159977.1
Mus musculus (house mouse) Tuba1a NP_035783.1
Rattus norvegicus (Norway rat) Tuba1a NP_071634.1
Gallus gallus (chicken) TUBA3E XP_419249.3
Danio rerio (zebrafish) tuba1l2 NP_001177911.1
Caenorhabditis elegans tba-5 NP_492268.1
Xenopus (Silurana) tropicalis (western clawed frog) tuba1b XP_002935154.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TUBA1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TUBA1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu23600 NM_006009 Homo sapiens tubulin, alpha 1a (TUBA1A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu23600 NM_001270399 Homo sapiens tubulin, alpha 1a (TUBA1A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu23702 NM_001270400 Homo sapiens tubulin, alpha 1a (TUBA1A), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23600
Clone ID Related Accession (Same CDS sequence) NM_001270399 , NM_006009
Accession Version NM_006009.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tubulin alpha-1A chain isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC340080.1, BC006468.1 and BC057823.1. This sequence is a reference standard in the RefSeqGene project. On Jul 4, 2012 this sequence version replaced gi:17986282. Transcript Variant: This variant (1) represents the shortest transcript and encodes isoform 1. Variants 1 and 2 encode the same protein (isoform 1) but use distinct start codons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057823.1, BY794954.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGAGT GCATCTCCAT CCACGTTGGC CAGGCTGGTG TCCAGATTGG CAATGCCTGC 
TGGGAGCTCT ACTGCCTGGA ACACGGCATC CAGCCCGATG GCCAGATGCC AAGTGACAAG
ACCATTGGGG GAGGAGATGA TTCCTTCAAC ACCTTCTTCA GTGAGACGGG GGCTGGCAAG
CATGTGCCCC GGGCAGTGTT TGTAGACTTG GAACCCACAG TCATTGATGA AGTTCGCACT
GGCACCTACC GCCAGCTCTT CCACCCTGAG CAACTTATCA CAGGCAAAGA AGATGCTGCC
AATAACTATG CCCGAGGGCA CTACACCATT GGCAAGGAGA TCATTGACCT CGTGTTGGAC
CGAATTCGCA AGCTGGCCGA CCAGTGCACG GGTCTCCAGG GCTTCTTGGT TTTCCACAGC
TTTGGTGGGG GAACTGGTTC TGGGTTCACC TCGCTGCTCA TGGAACGTCT CTCAGTTGAT
TATGGCAAGA AGTCCAAGCT GGAGTTCTCT ATTTACCCGG CGCCCCAGGT TTCCACAGCT
GTAGTTGAGC CCTACAACTC CATCCTCACC ACCCACACCA CCCTGGAGCA CTCTGATTGT
GCCTTCATGG TAGACAATGA GGCCATCTAT GACATCTGTC GTAGAAACCT CGATATTGAG
CGTCCAACCT ATACTAACCT GAATAGGTTA ATAGGTCAAA TTGTGTCCTC CATCACTGCT
TCCCTGAGAT TTGATGGAGC CCTGAATGTT GACCTGACAG AATTCCAGAC CAACCTGGTG
CCCTATCCCC GCATCCACTT CCCTCTGGCC ACATATGCCC CTGTCATCTC TGCTGAGAAA
GCCTACCATG AACAGCTTTC TGTAGCAGAG ATCACCAATG CTTGCTTTGA GCCAGCCAAC
CAGATGGTGA AATGTGACCC TCGCCATGGT AAATACATGG CTTGCTGCCT GTTGTACCGT
GGTGACGTGG TTCCCAAAGA TGTCAATGCT GCCATTGCCA CCATCAAGAC CAAGCGTACC
ATCCAGTTTG TGGATTGGTG CCCCACTGGC TTCAAGGTTG GCATCAACTA CCAGCCTCCC
ACTGTGGTGC CTGGTGGAGA CCTGGCCAAG GTACAGAGAG CTGTGTGCAT GCTGAGCAAC
ACCACAGCCA TTGCTGAGGC CTGGGCTCGC CTGGACCACA AGTTTGACCT GATGTATGCC
AAACGTGCCT TTGTTCACTG GTACGTTGGG GAGGGGATGG AGGAAGGTGA GTTTTCAGAG
GCCCGTGAGG ACATGGCTGC CCTTGAGAAG GATTATGAGG AGGTTGGTGT GGATTCTGTT
GAAGGAGAGG GTGAGGAAGA AGGAGAGGAA TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006000.2
CDS346..1701
Misc Feature(1)283..285(+)
Misc Feature(2)346..1665(+)
Misc Feature(3)349..1650(+)
Misc Feature(4)349..1401(+)
Misc Feature(5)376..1029(+)
Misc Feature(6)559..1017(+)
Misc Feature(7)1660..1662(+)
Misc Feature(8)1696..1698(+)
Misc Feature(9)1696..1698(+)
Exon (1)1..348
Gene:TUBA1A
Gene Synonym:
Exon (2)349..571
Gene:TUBA1A
Gene Synonym:
Exon (3)572..720
Gene:TUBA1A
Gene Synonym:
Exon (4)721..1916
Gene:TUBA1A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006009
Organism Homo sapiens (human)
Definition Homo sapiens tubulin, alpha 1a (TUBA1A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006009

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGAGT GCATCTCCAT CCACGTTGGC CAGGCTGGTG TCCAGATTGG CAATGCCTGC 
TGGGAGCTCT ACTGCCTGGA ACACGGCATC CAGCCCGATG GCCAGATGCC AAGTGACAAG
ACCATTGGGG GAGGAGATGA TTCCTTCAAC ACCTTCTTCA GTGAGACGGG GGCTGGCAAG
CATGTGCCCC GGGCAGTGTT TGTAGACTTG GAACCCACAG TCATTGATGA AGTTCGCACT
GGCACCTACC GCCAGCTCTT CCACCCTGAG CAACTTATCA CAGGCAAAGA AGATGCTGCC
AATAACTATG CCCGAGGGCA CTACACCATT GGCAAGGAGA TCATTGACCT CGTGTTGGAC
CGAATTCGCA AGCTGGCCGA CCAGTGCACG GGTCTCCAGG GCTTCTTGGT TTTCCACAGC
TTTGGTGGGG GAACTGGTTC TGGGTTCACC TCGCTGCTCA TGGAACGTCT CTCAGTTGAT
TATGGCAAGA AGTCCAAGCT GGAGTTCTCT ATTTACCCGG CGCCCCAGGT TTCCACAGCT
GTAGTTGAGC CCTACAACTC CATCCTCACC ACCCACACCA CCCTGGAGCA CTCTGATTGT
GCCTTCATGG TAGACAATGA GGCCATCTAT GACATCTGTC GTAGAAACCT CGATATTGAG
CGTCCAACCT ATACTAACCT GAATAGGTTA ATAGGTCAAA TTGTGTCCTC CATCACTGCT
TCCCTGAGAT TTGATGGAGC CCTGAATGTT GACCTGACAG AATTCCAGAC CAACCTGGTG
CCCTATCCCC GCATCCACTT CCCTCTGGCC ACATATGCCC CTGTCATCTC TGCTGAGAAA
GCCTACCATG AACAGCTTTC TGTAGCAGAG ATCACCAATG CTTGCTTTGA GCCAGCCAAC
CAGATGGTGA AATGTGACCC TCGCCATGGT AAATACATGG CTTGCTGCCT GTTGTACCGT
GGTGACGTGG TTCCCAAAGA TGTCAATGCT GCCATTGCCA CCATCAAGAC CAAGCGTACC
ATCCAGTTTG TGGATTGGTG CCCCACTGGC TTCAAGGTTG GCATCAACTA CCAGCCTCCC
ACTGTGGTGC CTGGTGGAGA CCTGGCCAAG GTACAGAGAG CTGTGTGCAT GCTGAGCAAC
ACCACAGCCA TTGCTGAGGC CTGGGCTCGC CTGGACCACA AGTTTGACCT GATGTATGCC
AAACGTGCCT TTGTTCACTG GTACGTTGGG GAGGGGATGG AGGAAGGTGA GTTTTCAGAG
GCCCGTGAGG ACATGGCTGC CCTTGAGAAG GATTATGAGG AGGTTGGTGT GGATTCTGTT
GAAGGAGAGG GTGAGGAAGA AGGAGAGGAA TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23600
Clone ID Related Accession (Same CDS sequence) NM_001270399 , NM_006009
Accession Version NM_001270399.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tubulin alpha-1A chain isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC340080.1, DA117857.1, AK289483.1, BC006468.1 and BC057823.1. Transcript Variant: This variant (2) contains an alternate segment at its 5' end which results in the use of a different start codon, compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1) but use distinct start codons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK289483.1, BQ721806.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGAGT GCATCTCCAT CCACGTTGGC CAGGCTGGTG TCCAGATTGG CAATGCCTGC 
TGGGAGCTCT ACTGCCTGGA ACACGGCATC CAGCCCGATG GCCAGATGCC AAGTGACAAG
ACCATTGGGG GAGGAGATGA TTCCTTCAAC ACCTTCTTCA GTGAGACGGG GGCTGGCAAG
CATGTGCCCC GGGCAGTGTT TGTAGACTTG GAACCCACAG TCATTGATGA AGTTCGCACT
GGCACCTACC GCCAGCTCTT CCACCCTGAG CAACTTATCA CAGGCAAAGA AGATGCTGCC
AATAACTATG CCCGAGGGCA CTACACCATT GGCAAGGAGA TCATTGACCT CGTGTTGGAC
CGAATTCGCA AGCTGGCCGA CCAGTGCACG GGTCTCCAGG GCTTCTTGGT TTTCCACAGC
TTTGGTGGGG GAACTGGTTC TGGGTTCACC TCGCTGCTCA TGGAACGTCT CTCAGTTGAT
TATGGCAAGA AGTCCAAGCT GGAGTTCTCT ATTTACCCGG CGCCCCAGGT TTCCACAGCT
GTAGTTGAGC CCTACAACTC CATCCTCACC ACCCACACCA CCCTGGAGCA CTCTGATTGT
GCCTTCATGG TAGACAATGA GGCCATCTAT GACATCTGTC GTAGAAACCT CGATATTGAG
CGTCCAACCT ATACTAACCT GAATAGGTTA ATAGGTCAAA TTGTGTCCTC CATCACTGCT
TCCCTGAGAT TTGATGGAGC CCTGAATGTT GACCTGACAG AATTCCAGAC CAACCTGGTG
CCCTATCCCC GCATCCACTT CCCTCTGGCC ACATATGCCC CTGTCATCTC TGCTGAGAAA
GCCTACCATG AACAGCTTTC TGTAGCAGAG ATCACCAATG CTTGCTTTGA GCCAGCCAAC
CAGATGGTGA AATGTGACCC TCGCCATGGT AAATACATGG CTTGCTGCCT GTTGTACCGT
GGTGACGTGG TTCCCAAAGA TGTCAATGCT GCCATTGCCA CCATCAAGAC CAAGCGTACC
ATCCAGTTTG TGGATTGGTG CCCCACTGGC TTCAAGGTTG GCATCAACTA CCAGCCTCCC
ACTGTGGTGC CTGGTGGAGA CCTGGCCAAG GTACAGAGAG CTGTGTGCAT GCTGAGCAAC
ACCACAGCCA TTGCTGAGGC CTGGGCTCGC CTGGACCACA AGTTTGACCT GATGTATGCC
AAACGTGCCT TTGTTCACTG GTACGTTGGG GAGGGGATGG AGGAAGGTGA GTTTTCAGAG
GCCCGTGAGG ACATGGCTGC CCTTGAGAAG GATTATGAGG AGGTTGGTGT GGATTCTGTT
GAAGGAGAGG GTGAGGAAGA AGGAGAGGAA TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001257328.1
CDS906..2261
Misc Feature(1)840..842(+)
Misc Feature(2)906..2225(+)
Misc Feature(3)909..2210(+)
Misc Feature(4)909..1961(+)
Misc Feature(5)936..1589(+)
Misc Feature(6)1119..1577(+)
Misc Feature(7)2256..2258(+)
Exon (1)1..908
Gene:TUBA1A
Gene Synonym:
Exon (2)909..1131
Gene:TUBA1A
Gene Synonym:
Exon (3)1132..1280
Gene:TUBA1A
Gene Synonym:
Exon (4)1281..2476
Gene:TUBA1A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001270399
Organism Homo sapiens (human)
Definition Homo sapiens tubulin, alpha 1a (TUBA1A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001270399

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGAGT GCATCTCCAT CCACGTTGGC CAGGCTGGTG TCCAGATTGG CAATGCCTGC 
TGGGAGCTCT ACTGCCTGGA ACACGGCATC CAGCCCGATG GCCAGATGCC AAGTGACAAG
ACCATTGGGG GAGGAGATGA TTCCTTCAAC ACCTTCTTCA GTGAGACGGG GGCTGGCAAG
CATGTGCCCC GGGCAGTGTT TGTAGACTTG GAACCCACAG TCATTGATGA AGTTCGCACT
GGCACCTACC GCCAGCTCTT CCACCCTGAG CAACTTATCA CAGGCAAAGA AGATGCTGCC
AATAACTATG CCCGAGGGCA CTACACCATT GGCAAGGAGA TCATTGACCT CGTGTTGGAC
CGAATTCGCA AGCTGGCCGA CCAGTGCACG GGTCTCCAGG GCTTCTTGGT TTTCCACAGC
TTTGGTGGGG GAACTGGTTC TGGGTTCACC TCGCTGCTCA TGGAACGTCT CTCAGTTGAT
TATGGCAAGA AGTCCAAGCT GGAGTTCTCT ATTTACCCGG CGCCCCAGGT TTCCACAGCT
GTAGTTGAGC CCTACAACTC CATCCTCACC ACCCACACCA CCCTGGAGCA CTCTGATTGT
GCCTTCATGG TAGACAATGA GGCCATCTAT GACATCTGTC GTAGAAACCT CGATATTGAG
CGTCCAACCT ATACTAACCT GAATAGGTTA ATAGGTCAAA TTGTGTCCTC CATCACTGCT
TCCCTGAGAT TTGATGGAGC CCTGAATGTT GACCTGACAG AATTCCAGAC CAACCTGGTG
CCCTATCCCC GCATCCACTT CCCTCTGGCC ACATATGCCC CTGTCATCTC TGCTGAGAAA
GCCTACCATG AACAGCTTTC TGTAGCAGAG ATCACCAATG CTTGCTTTGA GCCAGCCAAC
CAGATGGTGA AATGTGACCC TCGCCATGGT AAATACATGG CTTGCTGCCT GTTGTACCGT
GGTGACGTGG TTCCCAAAGA TGTCAATGCT GCCATTGCCA CCATCAAGAC CAAGCGTACC
ATCCAGTTTG TGGATTGGTG CCCCACTGGC TTCAAGGTTG GCATCAACTA CCAGCCTCCC
ACTGTGGTGC CTGGTGGAGA CCTGGCCAAG GTACAGAGAG CTGTGTGCAT GCTGAGCAAC
ACCACAGCCA TTGCTGAGGC CTGGGCTCGC CTGGACCACA AGTTTGACCT GATGTATGCC
AAACGTGCCT TTGTTCACTG GTACGTTGGG GAGGGGATGG AGGAAGGTGA GTTTTCAGAG
GCCCGTGAGG ACATGGCTGC CCTTGAGAAG GATTATGAGG AGGTTGGTGT GGATTCTGTT
GAAGGAGAGG GTGAGGAAGA AGGAGAGGAA TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23702
Clone ID Related Accession (Same CDS sequence) NM_001270400
Accession Version NM_001270400.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1251bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tubulin alpha-1A chain isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC340080.1, AU136397.2, BC006468.1 and BC057823.1. Transcript Variant: This variant (3) contains an alternate segment at its 5' end which results in the use of an in-frame downstream start codon, compared to variant 1. This variant encodes a protein (isoform 2) with a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ951315.1, AU136397.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCAAGTG ACAAGACCAT TGGGGGAGGA GATGATTCCT TCAACACCTT CTTCAGTGAG 
ACGGGGGCTG GCAAGCATGT GCCCCGGGCA GTGTTTGTAG ACTTGGAACC CACAGTCATT
GATGAAGTTC GCACTGGCAC CTACCGCCAG CTCTTCCACC CTGAGCAACT TATCACAGGC
AAAGAAGATG CTGCCAATAA CTATGCCCGA GGGCACTACA CCATTGGCAA GGAGATCATT
GACCTCGTGT TGGACCGAAT TCGCAAGCTG GCCGACCAGT GCACGGGTCT CCAGGGCTTC
TTGGTTTTCC ACAGCTTTGG TGGGGGAACT GGTTCTGGGT TCACCTCGCT GCTCATGGAA
CGTCTCTCAG TTGATTATGG CAAGAAGTCC AAGCTGGAGT TCTCTATTTA CCCGGCGCCC
CAGGTTTCCA CAGCTGTAGT TGAGCCCTAC AACTCCATCC TCACCACCCA CACCACCCTG
GAGCACTCTG ATTGTGCCTT CATGGTAGAC AATGAGGCCA TCTATGACAT CTGTCGTAGA
AACCTCGATA TTGAGCGTCC AACCTATACT AACCTGAATA GGTTAATAGG TCAAATTGTG
TCCTCCATCA CTGCTTCCCT GAGATTTGAT GGAGCCCTGA ATGTTGACCT GACAGAATTC
CAGACCAACC TGGTGCCCTA TCCCCGCATC CACTTCCCTC TGGCCACATA TGCCCCTGTC
ATCTCTGCTG AGAAAGCCTA CCATGAACAG CTTTCTGTAG CAGAGATCAC CAATGCTTGC
TTTGAGCCAG CCAACCAGAT GGTGAAATGT GACCCTCGCC ATGGTAAATA CATGGCTTGC
TGCCTGTTGT ACCGTGGTGA CGTGGTTCCC AAAGATGTCA ATGCTGCCAT TGCCACCATC
AAGACCAAGC GTACCATCCA GTTTGTGGAT TGGTGCCCCA CTGGCTTCAA GGTTGGCATC
AACTACCAGC CTCCCACTGT GGTGCCTGGT GGAGACCTGG CCAAGGTACA GAGAGCTGTG
TGCATGCTGA GCAACACCAC AGCCATTGCT GAGGCCTGGG CTCGCCTGGA CCACAAGTTT
GACCTGATGT ATGCCAAACG TGCCTTTGTT CACTGGTACG TTGGGGAGGG GATGGAGGAA
GGTGAGTTTT CAGAGGCCCG TGAGGACATG GCTGCCCTTG AGAAGGATTA TGAGGAGGTT
GGTGTGGATT CTGTTGAAGG AGAGGGTGAG GAAGAAGGAG AGGAATACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001257329.1
CDS539..1789
Misc Feature(1)350..352(+)
Misc Feature(2)539..1753(+)
Misc Feature(3)539..1738(+)
Misc Feature(4)644..1117(+)
Misc Feature(5)647..1105(+)
Misc Feature(6)1028..1489(+)
Exon (1)1..436
Gene:TUBA1A
Gene Synonym:
Exon (2)437..659
Gene:TUBA1A
Gene Synonym:
Exon (3)660..808
Gene:TUBA1A
Gene Synonym:
Exon (4)809..2004
Gene:TUBA1A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001270400
Organism Homo sapiens (human)
Definition Homo sapiens tubulin, alpha 1a (TUBA1A), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001270400

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCAAGTG ACAAGACCAT TGGGGGAGGA GATGATTCCT TCAACACCTT CTTCAGTGAG 
ACGGGGGCTG GCAAGCATGT GCCCCGGGCA GTGTTTGTAG ACTTGGAACC CACAGTCATT
GATGAAGTTC GCACTGGCAC CTACCGCCAG CTCTTCCACC CTGAGCAACT TATCACAGGC
AAAGAAGATG CTGCCAATAA CTATGCCCGA GGGCACTACA CCATTGGCAA GGAGATCATT
GACCTCGTGT TGGACCGAAT TCGCAAGCTG GCCGACCAGT GCACGGGTCT CCAGGGCTTC
TTGGTTTTCC ACAGCTTTGG TGGGGGAACT GGTTCTGGGT TCACCTCGCT GCTCATGGAA
CGTCTCTCAG TTGATTATGG CAAGAAGTCC AAGCTGGAGT TCTCTATTTA CCCGGCGCCC
CAGGTTTCCA CAGCTGTAGT TGAGCCCTAC AACTCCATCC TCACCACCCA CACCACCCTG
GAGCACTCTG ATTGTGCCTT CATGGTAGAC AATGAGGCCA TCTATGACAT CTGTCGTAGA
AACCTCGATA TTGAGCGTCC AACCTATACT AACCTGAATA GGTTAATAGG TCAAATTGTG
TCCTCCATCA CTGCTTCCCT GAGATTTGAT GGAGCCCTGA ATGTTGACCT GACAGAATTC
CAGACCAACC TGGTGCCCTA TCCCCGCATC CACTTCCCTC TGGCCACATA TGCCCCTGTC
ATCTCTGCTG AGAAAGCCTA CCATGAACAG CTTTCTGTAG CAGAGATCAC CAATGCTTGC
TTTGAGCCAG CCAACCAGAT GGTGAAATGT GACCCTCGCC ATGGTAAATA CATGGCTTGC
TGCCTGTTGT ACCGTGGTGA CGTGGTTCCC AAAGATGTCA ATGCTGCCAT TGCCACCATC
AAGACCAAGC GTACCATCCA GTTTGTGGAT TGGTGCCCCA CTGGCTTCAA GGTTGGCATC
AACTACCAGC CTCCCACTGT GGTGCCTGGT GGAGACCTGG CCAAGGTACA GAGAGCTGTG
TGCATGCTGA GCAACACCAC AGCCATTGCT GAGGCCTGGG CTCGCCTGGA CCACAAGTTT
GACCTGATGT ATGCCAAACG TGCCTTTGTT CACTGGTACG TTGGGGAGGG GATGGAGGAA
GGTGAGTTTT CAGAGGCCCG TGAGGACATG GCTGCCCTTG AGAAGGATTA TGAGGAGGTT
GGTGTGGATT CTGTTGAAGG AGAGGGTGAG GAAGAAGGAG AGGAATACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Brain malformations and mutations in alpha- and beta-tubulin genes: a review of the literature and description of two new cases
Dev Med Child Neurol 56 (4), 354-360 (2014)
Romaniello R, Arrigoni F, Cavallini A, Tenderini E, Baschirotto C, Triulzi F, Bassi MT and Borgatti R.


book

Tubulin-interactive stilbene derivatives as anticancer agents
Cell. Mol. Biol. Lett. 18 (3), 368-397 (2013)
Mikstacka R, Stefanski T and Rozanski J.


book

Expanding the spectrum of TUBA1A-related cortical dysgenesis to Polymicrogyria
Eur. J. Hum. Genet. 21 (4), 381-385 (2013)
Poirier K, Saillour Y, Fourniol F, Francis F, Souville I, Valence S, Desguerre I, Marie Lepage J, Boddaert N, Line Jacquemont M, Beldjord C, Chelly J and Bahi-Buisson N.


book

Lissencephaly with marked ventricular dilation, agenesis of corpus callosum, and cerebellar hypoplasia caused by TUBA1A mutation
Brain Dev. 35 (3), 274-279 (2013)
Okumura A, Hayashi M, Tsurui H, Yamakawa Y, Abe S, Kudo T, Suzuki R, Shimizu T, Shimojima K and Yamamoto T.


book

Overlapping cortical malformations and mutations in TUBB2B and TUBA1A
Brain 136 (PT 2), 536-548 (2013)
Cushion TD, Dobyns WB, Mullins JG, Stoodley N, Chung SK, Fry AE, Hehr U, Gunny R, Aylsworth AS, Prabhakar P, Uyanik G, Rankin J, Rees MI and Pilz DT.


book

Reduction of microtubule catastrophe events by LIS1, platelet-activating factor acetylhydrolase subunit
EMBO J. 16 (23), 6977-6984 (1997)
Sapir T, Elbaum M and Reiner O.


book

The N-terminal domain of c-Myc associates with alpha-tubulin and microtubules in vivo and in vitro
Mol. Cell. Biol. 15 (9), 5188-5195 (1995)
Alexandrova N, Niklinski J, Bliskovsky V, Otterson GA, Blake M, Kaye FJ and Zajac-Kaye M.


book

Cell cycle regulation of the activity and subcellular localization of Plk1, a human protein kinase implicated in mitotic spindle function
J. Cell Biol. 129 (6), 1617-1628 (1995)
Golsteyn RM, Mundt KE, Fry AM and Nigg EA.


book

Structural features and restricted expression of a human alpha-tubulin gene
Nucleic Acids Res. 13 (1), 207-223 (1985)
Hall,J.L. and Cowan,N.J.


book

Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions
Mol. Cell. Biol. 3 (10), 1738-1745 (1983)
Cowan,N.J., Dobner,P.R., Fuchs,E.V. and Cleveland,D.W.


 
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