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TUBA1A tubulin, alpha 1a [Homo sapiens (human)]

Gene Symbol TUBA1A
Entrez Gene ID 7846
Full Name tubulin, alpha 1a
Synonyms B-ALPHA-1, LIS3, TUBA3
General protein information
Preferred Names
tubulin alpha-1A chain
Names
tubulin alpha-1A chain
hum-a-tub1
hum-a-tub2
tubulin B-alpha-1
tubulin alpha-3 chain
tubulin, alpha, brain-specific
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q13.12

Summary Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]. lac of sum
Disorder MIM:

602529

Disorder Html: Lissencephaly 3, 611603 (3)





The following TUBA1A gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TUBA1A gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu23600 NM_006009 Homo sapiens tubulin, alpha 1a (TUBA1A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu23600 NM_001270399 Homo sapiens tubulin, alpha 1a (TUBA1A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu23702 NM_001270400 Homo sapiens tubulin, alpha 1a (TUBA1A), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu23600
Accession Version NM_006009.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tubulin alpha-1A chain isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC340080.1, BC006468.1 and BC057823.1. This sequence is a reference standard in the RefSeqGene project. On Jul 4, 2012 this sequence version replaced gi:17986282. Summary: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]. Transcript Variant: This variant (1) represents the shortest transcript and encodes isoform 1. Variants 1 and 2 encode the same protein (isoform 1) but use distinct start codons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057823.1, BY794954.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGAGT GCATCTCCAT CCACGTTGGC CAGGCTGGTG TCCAGATTGG CAATGCCTGC 
TGGGAGCTCT ACTGCCTGGA ACACGGCATC CAGCCCGATG GCCAGATGCC AAGTGACAAG
ACCATTGGGG GAGGAGATGA TTCCTTCAAC ACCTTCTTCA GTGAGACGGG GGCTGGCAAG
CATGTGCCCC GGGCAGTGTT TGTAGACTTG GAACCCACAG TCATTGATGA AGTTCGCACT
GGCACCTACC GCCAGCTCTT CCACCCTGAG CAACTTATCA CAGGCAAAGA AGATGCTGCC
AATAACTATG CCCGAGGGCA CTACACCATT GGCAAGGAGA TCATTGACCT CGTGTTGGAC
CGAATTCGCA AGCTGGCCGA CCAGTGCACG GGTCTCCAGG GCTTCTTGGT TTTCCACAGC
TTTGGTGGGG GAACTGGTTC TGGGTTCACC TCGCTGCTCA TGGAACGTCT CTCAGTTGAT
TATGGCAAGA AGTCCAAGCT GGAGTTCTCT ATTTACCCGG CGCCCCAGGT TTCCACAGCT
GTAGTTGAGC CCTACAACTC CATCCTCACC ACCCACACCA CCCTGGAGCA CTCTGATTGT
GCCTTCATGG TAGACAATGA GGCCATCTAT GACATCTGTC GTAGAAACCT CGATATTGAG
CGTCCAACCT ATACTAACCT GAATAGGTTA ATAGGTCAAA TTGTGTCCTC CATCACTGCT
TCCCTGAGAT TTGATGGAGC CCTGAATGTT GACCTGACAG AATTCCAGAC CAACCTGGTG
CCCTATCCCC GCATCCACTT CCCTCTGGCC ACATATGCCC CTGTCATCTC TGCTGAGAAA
GCCTACCATG AACAGCTTTC TGTAGCAGAG ATCACCAATG CTTGCTTTGA GCCAGCCAAC
CAGATGGTGA AATGTGACCC TCGCCATGGT AAATACATGG CTTGCTGCCT GTTGTACCGT
GGTGACGTGG TTCCCAAAGA TGTCAATGCT GCCATTGCCA CCATCAAGAC CAAGCGTACC
ATCCAGTTTG TGGATTGGTG CCCCACTGGC TTCAAGGTTG GCATCAACTA CCAGCCTCCC
ACTGTGGTGC CTGGTGGAGA CCTGGCCAAG GTACAGAGAG CTGTGTGCAT GCTGAGCAAC
ACCACAGCCA TTGCTGAGGC CTGGGCTCGC CTGGACCACA AGTTTGACCT GATGTATGCC
AAACGTGCCT TTGTTCACTG GTACGTTGGG GAGGGGATGG AGGAAGGTGA GTTTTCAGAG
GCCCGTGAGG ACATGGCTGC CCTTGAGAAG GATTATGAGG AGGTTGGTGT GGATTCTGTT
GAAGGAGAGG GTGAGGAAGA AGGAGAGGAA TACTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_006000.2
CDS346..1701
Misc Feature(1)283..285(+)
Misc Feature(2)346..1665(+)
Misc Feature(3)349..1650(+)
Misc Feature(4)349..1401(+)
Misc Feature(5)376..1029(+)
Misc Feature(6)559..1017(+)
Misc Feature(7)1660..1662(+)
Misc Feature(8)1696..1698(+)
Misc Feature(9)1696..1698(+)
Exon (1)1..348
Gene:TUBA1A
Gene Synonym:
Exon (2)349..571
Gene:TUBA1A
Gene Synonym:
Exon (3)572..720
Gene:TUBA1A
Gene Synonym:
Exon (4)721..1916
Gene:TUBA1A
Gene Synonym:
Translation
Position Chain Variation Link
14 14 c, g dbSNP:763523023
45 45 a, g dbSNP:753215197
63 63 a, g dbSNP:192252132
69 69 a, g dbSNP:765829618
86 86 a, g dbSNP:548167433
97 97 c, t dbSNP:531238602
110 110 c, g dbSNP:562588719
112 112 c, g dbSNP:187974148
158 158 c, t dbSNP:545598107
169 169 c, g dbSNP:532306297
222 222 a, g dbSNP:760023279
223 223 a, g dbSNP:559919161
224 224 a, c dbSNP:540076934
268 268 a, c, t dbSNP:574389302
272 272 g, t dbSNP:554470192
277 277 a, g dbSNP:371310839
289 289 a, g dbSNP:182661252
292 292 a, g dbSNP:11558063
294 294 -, aac dbSNP:751720598
302 302 -, ctc dbSNP:766250512
303 303 c, t dbSNP:767287078
304 304 c, t dbSNP:761622371
306 306 c, t dbSNP:773953696
307 307 a, c, t dbSNP:11558074
313 313 c, t dbSNP:762428394
316 316 c, t dbSNP:775124314
324 324 a, c dbSNP:770196685
325 325 c, g, t dbSNP:777015483
331 331 c, t dbSNP:192151179
340 340 a, g dbSNP:747406864
342 342 a, c dbSNP:539551108
350 350 a, g dbSNP:587784491
354 354 a, g dbSNP:764828844
358 358 a, c dbSNP:387906840
369 369 c, t dbSNP:143047732
411 411 a, g dbSNP:375516850
414 414 c, g dbSNP:766977640
429 429 c, t dbSNP:761327879
441 441 c, t dbSNP:139102191
468 468 c, t dbSNP:200198307
477 477 a, t dbSNP:772518269
486 486 c, t dbSNP:762120179
488 488 a, c dbSNP:11558073
489 489 c, t dbSNP:372306943
497 497 c, t dbSNP:587784485
498 498 c, g dbSNP:11558072
507 507 a, t dbSNP:587784486
510 510 a, g dbSNP:1055904
516 516 g, t dbSNP:1055956
528 528 c, t dbSNP:774605003
534 534 c, t dbSNP:147273934
537 537 a, g dbSNP:749375580
591 591 c, t dbSNP:1134868
603 603 c, g dbSNP:775649551
618 618 a, g dbSNP:765483435
621 621 c, t dbSNP:1065671
621 621 c, t dbSNP:587784487
633 633 a, g dbSNP:1056875
654 654 c, t dbSNP:772038983
657 657 c, g dbSNP:747966442
675 675 c, t dbSNP:774370656
682 682 c, g dbSNP:769889742
693 693 c, t dbSNP:768297938
696 696 c, t dbSNP:749139359
699 699 a, g dbSNP:779834877
720 720 a, g dbSNP:755889624
723 723 c, t dbSNP:1135224
729 729 a, g dbSNP:201704429
732 732 c, t dbSNP:745462580
735 735 a, c, g dbSNP:1143559
736 736 a, g dbSNP:11558067
741 741 c, t dbSNP:1143560
753 753 a, g dbSNP:201587501
762 762 c, t dbSNP:11558068
771 771 c, t dbSNP:778119816
777 777 a, t dbSNP:758957354
783 783 g, t dbSNP:753052479
798 798 a, c, g dbSNP:697624
816 816 c, t dbSNP:753969793
826 826 g, t dbSNP:587784488
837 837 a, g dbSNP:566021671
849 849 a, g dbSNP:760653068
855 855 c, t dbSNP:112023543
861 861 c, t dbSNP:370227094
864 864 a, g dbSNP:200372205
866 866 c, t dbSNP:587784489
867 867 a, g dbSNP:61730859
870 870 a, c, t dbSNP:775583580
888 888 a, c dbSNP:544713824
891 891 a, t dbSNP:759405602
907 907 a, c dbSNP:137853045
909 909 -, atc dbSNP:587784490
910 910 c, t dbSNP:113208258
924 924 c, t dbSNP:140239444
933 933 a, g dbSNP:770665177
936 936 c, t dbSNP:746486977
941 941 a, g dbSNP:786205479
975 975 c, t dbSNP:777403500
987 987 c, t dbSNP:771571589
993 993 c, t dbSNP:748593535
996 996 c, t dbSNP:779416899
1002 1002 c, t dbSNP:146680997
1008 1008 c, t dbSNP:754280693
1020 1020 c, t dbSNP:780129528
1032 1032 a, g dbSNP:756428949
1043 1043 a, g dbSNP:587784492
1056 1056 c, t dbSNP:267603493
1103 1103 c, g dbSNP:587784493
1119 1119 c, t dbSNP:750614684
1125 1125 a, g dbSNP:767648261
1131 1131 c, t dbSNP:762008241
1132 1132 a, c dbSNP:137853046
1135 1135 c, t dbSNP:137853043
1152 1152 a, g dbSNP:752640192
1153 1153 g, t dbSNP:587784494
1164 1164 c, t dbSNP:140795162
1166 1166 -, c, ct dbSNP:34268631
1194 1194 c, t dbSNP:759228609
1204 1204 a, t dbSNP:150978489
1263 1263 c, t dbSNP:765938699
1272 1272 c, t dbSNP:760304736
1296 1296 c, g dbSNP:565144370
1297 1297 c, t dbSNP:771520376
1299 1299 a, g dbSNP:545234429
1311 1311 c, t dbSNP:142641191
1331 1331 a, g dbSNP:587784495
1335 1335 c, t dbSNP:774821065
1336 1336 a, g dbSNP:587784496
1338 1338 c, g dbSNP:769212646
1340 1340 c, t dbSNP:587784497
1341 1341 c, t dbSNP:148479477
1353 1353 a, g dbSNP:11829764
1389 1389 c, t dbSNP:144574983
1410 1410 c, t dbSNP:746054164
1413 1413 c, t dbSNP:781597673
1440 1440 c, t dbSNP:140737357
1458 1458 -, ta dbSNP:200449362
1473 1473 c, t dbSNP:751737187
1474 1474 a, g dbSNP:587784481
1493 1493 c, t dbSNP:587784482
1512 1512 c, t dbSNP:199813289
1535 1535 c, t dbSNP:137853048
1548 1548 a, g dbSNP:1065724
1549 1549 a, c, t dbSNP:587784483
1550 1550 a, g, t dbSNP:137853044
1569 1569 c, t dbSNP:753719501
1572 1572 g, t dbSNP:766135902
1575 1575 c, g, t dbSNP:772809398
1593 1593 c, t dbSNP:3206694
1599 1599 c, t dbSNP:201884190
1601 1601 c, t dbSNP:137853047
1603 1603 a, g dbSNP:11558065
1608 1608 c, g dbSNP:11558070
1609 1609 c, t dbSNP:137853049
1610 1610 a, g dbSNP:137853050
1611 1611 g, t dbSNP:761301881
1619 1619 a, t dbSNP:587784484
1625 1625 c, g dbSNP:773920941
1644 1644 a, g dbSNP:768060474
1663 1663 a, g dbSNP:749746325
1665 1665 c, t dbSNP:11558069
1674 1674 a, g dbSNP:775885354
1676 1676 c, g dbSNP:770156445
1680 1680 a, g dbSNP:746276826
1684 1684 a, g dbSNP:1065730
1692 1692 a, g dbSNP:200873943
1702 1702 -, a dbSNP:781483247
1705 1705 -, t dbSNP:768839661
1710 1710 c, t dbSNP:371718931
1711 1711 a, g dbSNP:747080229
1714 1714 a, c dbSNP:1062437
1717 1717 a, g dbSNP:777929464
1720 1720 a, g dbSNP:758417587
1723 1723 c, t dbSNP:753757160
1724 1724 c, t dbSNP:766255841
1727 1727 g, t dbSNP:185938303
1788 1788 a, g dbSNP:11558066
1807 1807 c, t dbSNP:1062439
1814 1814 a, g dbSNP:556755463
1815 1815 c, t dbSNP:150293856
1820 1820 -, a dbSNP:757230901
1825 1825 c, t dbSNP:571426354
1833 1833 c, t dbSNP:1062440
1847 1847 c, t dbSNP:557975791
1875 1875 c, t dbSNP:1062441
1903 1903 -, ct dbSNP:753859336
1912 1912 a, g dbSNP:140121590

Target ORF information:

RefSeq Version NM_006009
Organism Homo sapiens (human)
Definition Homo sapiens tubulin, alpha 1a (TUBA1A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006009

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGAGT GCATCTCCAT CCACGTTGGC CAGGCTGGTG TCCAGATTGG CAATGCCTGC 
TGGGAGCTCT ACTGCCTGGA ACACGGCATC CAGCCCGATG GCCAGATGCC AAGTGACAAG
ACCATTGGGG GAGGAGATGA TTCCTTCAAC ACCTTCTTCA GTGAGACGGG GGCTGGCAAG
CATGTGCCCC GGGCAGTGTT TGTAGACTTG GAACCCACAG TCATTGATGA AGTTCGCACT
GGCACCTACC GCCAGCTCTT CCACCCTGAG CAACTTATCA CAGGCAAAGA AGATGCTGCC
AATAACTATG CCCGAGGGCA CTACACCATT GGCAAGGAGA TCATTGACCT CGTGTTGGAC
CGAATTCGCA AGCTGGCCGA CCAGTGCACG GGTCTCCAGG GCTTCTTGGT TTTCCACAGC
TTTGGTGGGG GAACTGGTTC TGGGTTCACC TCGCTGCTCA TGGAACGTCT CTCAGTTGAT
TATGGCAAGA AGTCCAAGCT GGAGTTCTCT ATTTACCCGG CGCCCCAGGT TTCCACAGCT
GTAGTTGAGC CCTACAACTC CATCCTCACC ACCCACACCA CCCTGGAGCA CTCTGATTGT
GCCTTCATGG TAGACAATGA GGCCATCTAT GACATCTGTC GTAGAAACCT CGATATTGAG
CGTCCAACCT ATACTAACCT GAATAGGTTA ATAGGTCAAA TTGTGTCCTC CATCACTGCT
TCCCTGAGAT TTGATGGAGC CCTGAATGTT GACCTGACAG AATTCCAGAC CAACCTGGTG
CCCTATCCCC GCATCCACTT CCCTCTGGCC ACATATGCCC CTGTCATCTC TGCTGAGAAA
GCCTACCATG AACAGCTTTC TGTAGCAGAG ATCACCAATG CTTGCTTTGA GCCAGCCAAC
CAGATGGTGA AATGTGACCC TCGCCATGGT AAATACATGG CTTGCTGCCT GTTGTACCGT
GGTGACGTGG TTCCCAAAGA TGTCAATGCT GCCATTGCCA CCATCAAGAC CAAGCGTACC
ATCCAGTTTG TGGATTGGTG CCCCACTGGC TTCAAGGTTG GCATCAACTA CCAGCCTCCC
ACTGTGGTGC CTGGTGGAGA CCTGGCCAAG GTACAGAGAG CTGTGTGCAT GCTGAGCAAC
ACCACAGCCA TTGCTGAGGC CTGGGCTCGC CTGGACCACA AGTTTGACCT GATGTATGCC
AAACGTGCCT TTGTTCACTG GTACGTTGGG GAGGGGATGG AGGAAGGTGA GTTTTCAGAG
GCCCGTGAGG ACATGGCTGC CCTTGAGAAG GATTATGAGG AGGTTGGTGT GGATTCTGTT
GAAGGAGAGG GTGAGGAAGA AGGAGAGGAA TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23600
Accession Version NM_001270399.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1356bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tubulin alpha-1A chain isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC340080.1, DA117857.1, AK289483.1, BC006468.1 and BC057823.1. Summary: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]. Transcript Variant: This variant (2) contains an alternate segment at its 5' end which results in the use of a different start codon, compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1) but use distinct start codons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK289483.1, BQ721806.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGAGT GCATCTCCAT CCACGTTGGC CAGGCTGGTG TCCAGATTGG CAATGCCTGC 
TGGGAGCTCT ACTGCCTGGA ACACGGCATC CAGCCCGATG GCCAGATGCC AAGTGACAAG
ACCATTGGGG GAGGAGATGA TTCCTTCAAC ACCTTCTTCA GTGAGACGGG GGCTGGCAAG
CATGTGCCCC GGGCAGTGTT TGTAGACTTG GAACCCACAG TCATTGATGA AGTTCGCACT
GGCACCTACC GCCAGCTCTT CCACCCTGAG CAACTTATCA CAGGCAAAGA AGATGCTGCC
AATAACTATG CCCGAGGGCA CTACACCATT GGCAAGGAGA TCATTGACCT CGTGTTGGAC
CGAATTCGCA AGCTGGCCGA CCAGTGCACG GGTCTCCAGG GCTTCTTGGT TTTCCACAGC
TTTGGTGGGG GAACTGGTTC TGGGTTCACC TCGCTGCTCA TGGAACGTCT CTCAGTTGAT
TATGGCAAGA AGTCCAAGCT GGAGTTCTCT ATTTACCCGG CGCCCCAGGT TTCCACAGCT
GTAGTTGAGC CCTACAACTC CATCCTCACC ACCCACACCA CCCTGGAGCA CTCTGATTGT
GCCTTCATGG TAGACAATGA GGCCATCTAT GACATCTGTC GTAGAAACCT CGATATTGAG
CGTCCAACCT ATACTAACCT GAATAGGTTA ATAGGTCAAA TTGTGTCCTC CATCACTGCT
TCCCTGAGAT TTGATGGAGC CCTGAATGTT GACCTGACAG AATTCCAGAC CAACCTGGTG
CCCTATCCCC GCATCCACTT CCCTCTGGCC ACATATGCCC CTGTCATCTC TGCTGAGAAA
GCCTACCATG AACAGCTTTC TGTAGCAGAG ATCACCAATG CTTGCTTTGA GCCAGCCAAC
CAGATGGTGA AATGTGACCC TCGCCATGGT AAATACATGG CTTGCTGCCT GTTGTACCGT
GGTGACGTGG TTCCCAAAGA TGTCAATGCT GCCATTGCCA CCATCAAGAC CAAGCGTACC
ATCCAGTTTG TGGATTGGTG CCCCACTGGC TTCAAGGTTG GCATCAACTA CCAGCCTCCC
ACTGTGGTGC CTGGTGGAGA CCTGGCCAAG GTACAGAGAG CTGTGTGCAT GCTGAGCAAC
ACCACAGCCA TTGCTGAGGC CTGGGCTCGC CTGGACCACA AGTTTGACCT GATGTATGCC
AAACGTGCCT TTGTTCACTG GTACGTTGGG GAGGGGATGG AGGAAGGTGA GTTTTCAGAG
GCCCGTGAGG ACATGGCTGC CCTTGAGAAG GATTATGAGG AGGTTGGTGT GGATTCTGTT
GAAGGAGAGG GTGAGGAAGA AGGAGAGGAA TACTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001257328.1
CDS906..2261
Misc Feature(1)840..842(+)
Misc Feature(2)906..2225(+)
Misc Feature(3)909..2210(+)
Misc Feature(4)909..1961(+)
Misc Feature(5)936..1589(+)
Misc Feature(6)1119..1577(+)
Misc Feature(7)2256..2258(+)
Exon (1)1..908
Gene:TUBA1A
Gene Synonym:
Exon (2)909..1131
Gene:TUBA1A
Gene Synonym:
Exon (3)1132..1280
Gene:TUBA1A
Gene Synonym:
Exon (4)1281..2476
Gene:TUBA1A
Gene Synonym:
Translation
Position Chain Variation Link
14 14 c, g dbSNP:763523023
45 45 a, g dbSNP:753215197
63 63 a, g dbSNP:192252132
69 69 a, g dbSNP:765829618
86 86 a, g dbSNP:548167433
97 97 c, t dbSNP:531238602
110 110 c, g dbSNP:562588719
112 112 c, g dbSNP:187974148
158 158 c, t dbSNP:545598107
169 169 c, g dbSNP:532306297
222 222 a, g dbSNP:760023279
223 223 a, g dbSNP:559919161
224 224 a, c dbSNP:540076934
268 268 a, c, t dbSNP:574389302
272 272 g, t dbSNP:554470192
277 277 a, g dbSNP:371310839
289 289 a, g dbSNP:182661252
292 292 a, g dbSNP:11558063
294 294 -, aac dbSNP:751720598
302 302 -, ctc dbSNP:766250512
303 303 c, t dbSNP:767287078
304 304 c, t dbSNP:761622371
306 306 c, t dbSNP:773953696
307 307 a, c, t dbSNP:11558074
313 313 c, t dbSNP:762428394
316 316 c, t dbSNP:775124314
324 324 a, c dbSNP:770196685
325 325 c, g, t dbSNP:777015483
331 331 c, t dbSNP:192151179
340 340 a, g dbSNP:747406864
342 342 a, c dbSNP:539551108
351 351 a, g dbSNP:758738979
358 358 c, g dbSNP:748160639
360 360 c, t dbSNP:778988809
364 364 a, c, g dbSNP:750328538
366 366 c, t dbSNP:767387627
369 369 c, t dbSNP:757016133
371 371 c, t dbSNP:751404305
375 375 a, g dbSNP:763724020
377 377 c, t dbSNP:762660170
378 378 -, g dbSNP:146808943
378 378 g, t dbSNP:775008245
379 379 g, t dbSNP:764746345
393 393 a, c dbSNP:758990710
395 395 a, c dbSNP:774938419
406 406 c, t dbSNP:368699278
416 416 a, c dbSNP:1039225
425 425 c, t dbSNP:139383482
430 430 c, t dbSNP:747433806
433 433 c, t dbSNP:534443774
451 451 c, t dbSNP:35896524
492 492 a, g dbSNP:554749892
516 516 -, cgc dbSNP:560742601
546 546 c, g dbSNP:747689090
551 551 c, t dbSNP:548637263
565 565 a, g dbSNP:531303096
579 579 c, g dbSNP:568783692
645 645 c, t dbSNP:756302997
648 648 a, g dbSNP:186298085
665 665 c, t dbSNP:532087034
670 670 c, g dbSNP:559861728
686 686 c, t dbSNP:758233469
719 719 a, g dbSNP:115188082
774 774 c, g dbSNP:80167147
782 782 a, g dbSNP:779777418
826 826 a, g dbSNP:772222238
837 837 g, t dbSNP:765224487
894 894 -, t dbSNP:758155234
903 903 a, c, t dbSNP:771601875
910 910 a, g dbSNP:587784491
914 914 a, g dbSNP:764828844
918 918 a, c dbSNP:387906840
929 929 c, t dbSNP:143047732
971 971 a, g dbSNP:375516850
974 974 c, g dbSNP:766977640
989 989 c, t dbSNP:761327879
1001 1001 c, t dbSNP:139102191
1028 1028 c, t dbSNP:200198307
1037 1037 a, t dbSNP:772518269
1046 1046 c, t dbSNP:762120179
1048 1048 a, c dbSNP:11558073
1049 1049 c, t dbSNP:372306943
1057 1057 c, t dbSNP:587784485
1058 1058 c, g dbSNP:11558072
1067 1067 a, t dbSNP:587784486
1070 1070 a, g dbSNP:1055904
1076 1076 g, t dbSNP:1055956
1088 1088 c, t dbSNP:774605003
1094 1094 c, t dbSNP:147273934
1097 1097 a, g dbSNP:749375580
1151 1151 c, t dbSNP:1134868
1163 1163 c, g dbSNP:775649551
1178 1178 a, g dbSNP:765483435
1181 1181 c, t dbSNP:1065671
1181 1181 c, t dbSNP:587784487
1193 1193 a, g dbSNP:1056875
1214 1214 c, t dbSNP:772038983
1217 1217 c, g dbSNP:747966442
1235 1235 c, t dbSNP:774370656
1242 1242 c, g dbSNP:769889742
1253 1253 c, t dbSNP:768297938
1256 1256 c, t dbSNP:749139359
1259 1259 a, g dbSNP:779834877
1280 1280 a, g dbSNP:755889624
1283 1283 c, t dbSNP:1135224
1289 1289 a, g dbSNP:201704429
1292 1292 c, t dbSNP:745462580
1295 1295 a, c, g dbSNP:1143559
1296 1296 a, g dbSNP:11558067
1301 1301 c, t dbSNP:1143560
1313 1313 a, g dbSNP:201587501
1322 1322 c, t dbSNP:11558068
1331 1331 c, t dbSNP:778119816
1337 1337 a, t dbSNP:758957354
1343 1343 g, t dbSNP:753052479
1358 1358 a, c, g dbSNP:697624
1376 1376 c, t dbSNP:753969793
1386 1386 g, t dbSNP:587784488
1397 1397 a, g dbSNP:566021671
1409 1409 a, g dbSNP:760653068
1415 1415 c, t dbSNP:112023543
1421 1421 c, t dbSNP:370227094
1424 1424 a, g dbSNP:200372205
1426 1426 c, t dbSNP:587784489
1427 1427 a, g dbSNP:61730859
1430 1430 a, c, t dbSNP:775583580
1448 1448 a, c dbSNP:544713824
1451 1451 a, t dbSNP:759405602
1467 1467 a, c dbSNP:137853045
1469 1469 -, atc dbSNP:587784490
1470 1470 c, t dbSNP:113208258
1484 1484 c, t dbSNP:140239444
1493 1493 a, g dbSNP:770665177
1496 1496 c, t dbSNP:746486977
1501 1501 a, g dbSNP:786205479
1535 1535 c, t dbSNP:777403500
1547 1547 c, t dbSNP:771571589
1553 1553 c, t dbSNP:748593535
1556 1556 c, t dbSNP:779416899
1562 1562 c, t dbSNP:146680997
1568 1568 c, t dbSNP:754280693
1580 1580 c, t dbSNP:780129528
1592 1592 a, g dbSNP:756428949
1603 1603 a, g dbSNP:587784492
1616 1616 c, t dbSNP:267603493
1663 1663 c, g dbSNP:587784493
1679 1679 c, t dbSNP:750614684
1685 1685 a, g dbSNP:767648261
1691 1691 c, t dbSNP:762008241
1692 1692 a, c dbSNP:137853046
1695 1695 c, t dbSNP:137853043
1712 1712 a, g dbSNP:752640192
1713 1713 g, t dbSNP:587784494
1724 1724 c, t dbSNP:140795162
1726 1726 -, c, ct dbSNP:34268631
1754 1754 c, t dbSNP:759228609
1764 1764 a, t dbSNP:150978489
1823 1823 c, t dbSNP:765938699
1832 1832 c, t dbSNP:760304736
1856 1856 c, g dbSNP:565144370
1857 1857 c, t dbSNP:771520376
1859 1859 a, g dbSNP:545234429
1871 1871 c, t dbSNP:142641191
1891 1891 a, g dbSNP:587784495
1895 1895 c, t dbSNP:774821065
1896 1896 a, g dbSNP:587784496
1898 1898 c, g dbSNP:769212646
1900 1900 c, t dbSNP:587784497
1901 1901 c, t dbSNP:148479477
1913 1913 a, g dbSNP:11829764
1949 1949 c, t dbSNP:144574983
1970 1970 c, t dbSNP:746054164
1973 1973 c, t dbSNP:781597673
2000 2000 c, t dbSNP:140737357
2018 2018 -, ta dbSNP:200449362
2033 2033 c, t dbSNP:751737187
2034 2034 a, g dbSNP:587784481
2053 2053 c, t dbSNP:587784482
2072 2072 c, t dbSNP:199813289
2095 2095 c, t dbSNP:137853048
2108 2108 a, g dbSNP:1065724
2109 2109 a, c, t dbSNP:587784483
2110 2110 a, g, t dbSNP:137853044
2129 2129 c, t dbSNP:753719501
2132 2132 g, t dbSNP:766135902
2135 2135 c, g, t dbSNP:772809398
2153 2153 c, t dbSNP:3206694
2159 2159 c, t dbSNP:201884190
2161 2161 c, t dbSNP:137853047
2163 2163 a, g dbSNP:11558065
2168 2168 c, g dbSNP:11558070
2169 2169 c, t dbSNP:137853049
2170 2170 a, g dbSNP:137853050
2171 2171 g, t dbSNP:761301881
2179 2179 a, t dbSNP:587784484
2185 2185 c, g dbSNP:773920941
2204 2204 a, g dbSNP:768060474
2223 2223 a, g dbSNP:749746325
2225 2225 c, t dbSNP:11558069
2234 2234 a, g dbSNP:775885354
2236 2236 c, g dbSNP:770156445
2240 2240 a, g dbSNP:746276826
2244 2244 a, g dbSNP:1065730
2252 2252 a, g dbSNP:200873943
2262 2262 -, a dbSNP:781483247
2265 2265 -, t dbSNP:768839661
2270 2270 c, t dbSNP:371718931
2271 2271 a, g dbSNP:747080229
2274 2274 a, c dbSNP:1062437
2277 2277 a, g dbSNP:777929464
2280 2280 a, g dbSNP:758417587
2283 2283 c, t dbSNP:753757160
2284 2284 c, t dbSNP:766255841
2287 2287 g, t dbSNP:185938303
2348 2348 a, g dbSNP:11558066
2367 2367 c, t dbSNP:1062439
2374 2374 a, g dbSNP:556755463
2375 2375 c, t dbSNP:150293856
2380 2380 -, a dbSNP:757230901
2385 2385 c, t dbSNP:571426354
2393 2393 c, t dbSNP:1062440
2407 2407 c, t dbSNP:557975791
2435 2435 c, t dbSNP:1062441
2463 2463 -, ct dbSNP:753859336
2472 2472 a, g dbSNP:140121590

Target ORF information:

RefSeq Version NM_001270399
Organism Homo sapiens (human)
Definition Homo sapiens tubulin, alpha 1a (TUBA1A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001270399

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGAGT GCATCTCCAT CCACGTTGGC CAGGCTGGTG TCCAGATTGG CAATGCCTGC 
TGGGAGCTCT ACTGCCTGGA ACACGGCATC CAGCCCGATG GCCAGATGCC AAGTGACAAG
ACCATTGGGG GAGGAGATGA TTCCTTCAAC ACCTTCTTCA GTGAGACGGG GGCTGGCAAG
CATGTGCCCC GGGCAGTGTT TGTAGACTTG GAACCCACAG TCATTGATGA AGTTCGCACT
GGCACCTACC GCCAGCTCTT CCACCCTGAG CAACTTATCA CAGGCAAAGA AGATGCTGCC
AATAACTATG CCCGAGGGCA CTACACCATT GGCAAGGAGA TCATTGACCT CGTGTTGGAC
CGAATTCGCA AGCTGGCCGA CCAGTGCACG GGTCTCCAGG GCTTCTTGGT TTTCCACAGC
TTTGGTGGGG GAACTGGTTC TGGGTTCACC TCGCTGCTCA TGGAACGTCT CTCAGTTGAT
TATGGCAAGA AGTCCAAGCT GGAGTTCTCT ATTTACCCGG CGCCCCAGGT TTCCACAGCT
GTAGTTGAGC CCTACAACTC CATCCTCACC ACCCACACCA CCCTGGAGCA CTCTGATTGT
GCCTTCATGG TAGACAATGA GGCCATCTAT GACATCTGTC GTAGAAACCT CGATATTGAG
CGTCCAACCT ATACTAACCT GAATAGGTTA ATAGGTCAAA TTGTGTCCTC CATCACTGCT
TCCCTGAGAT TTGATGGAGC CCTGAATGTT GACCTGACAG AATTCCAGAC CAACCTGGTG
CCCTATCCCC GCATCCACTT CCCTCTGGCC ACATATGCCC CTGTCATCTC TGCTGAGAAA
GCCTACCATG AACAGCTTTC TGTAGCAGAG ATCACCAATG CTTGCTTTGA GCCAGCCAAC
CAGATGGTGA AATGTGACCC TCGCCATGGT AAATACATGG CTTGCTGCCT GTTGTACCGT
GGTGACGTGG TTCCCAAAGA TGTCAATGCT GCCATTGCCA CCATCAAGAC CAAGCGTACC
ATCCAGTTTG TGGATTGGTG CCCCACTGGC TTCAAGGTTG GCATCAACTA CCAGCCTCCC
ACTGTGGTGC CTGGTGGAGA CCTGGCCAAG GTACAGAGAG CTGTGTGCAT GCTGAGCAAC
ACCACAGCCA TTGCTGAGGC CTGGGCTCGC CTGGACCACA AGTTTGACCT GATGTATGCC
AAACGTGCCT TTGTTCACTG GTACGTTGGG GAGGGGATGG AGGAAGGTGA GTTTTCAGAG
GCCCGTGAGG ACATGGCTGC CCTTGAGAAG GATTATGAGG AGGTTGGTGT GGATTCTGTT
GAAGGAGAGG GTGAGGAAGA AGGAGAGGAA TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23702
Accession Version NM_001270400.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1251bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tubulin alpha-1A chain isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC340080.1, AU136397.2, BC006468.1 and BC057823.1. Summary: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]. Transcript Variant: This variant (3) contains an alternate segment at its 5' end which results in the use of an in-frame downstream start codon, compared to variant 1. This variant encodes a protein (isoform 2) with a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ951315.1, AU136397.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCAAGTG ACAAGACCAT TGGGGGAGGA GATGATTCCT TCAACACCTT CTTCAGTGAG 
ACGGGGGCTG GCAAGCATGT GCCCCGGGCA GTGTTTGTAG ACTTGGAACC CACAGTCATT
GATGAAGTTC GCACTGGCAC CTACCGCCAG CTCTTCCACC CTGAGCAACT TATCACAGGC
AAAGAAGATG CTGCCAATAA CTATGCCCGA GGGCACTACA CCATTGGCAA GGAGATCATT
GACCTCGTGT TGGACCGAAT TCGCAAGCTG GCCGACCAGT GCACGGGTCT CCAGGGCTTC
TTGGTTTTCC ACAGCTTTGG TGGGGGAACT GGTTCTGGGT TCACCTCGCT GCTCATGGAA
CGTCTCTCAG TTGATTATGG CAAGAAGTCC AAGCTGGAGT TCTCTATTTA CCCGGCGCCC
CAGGTTTCCA CAGCTGTAGT TGAGCCCTAC AACTCCATCC TCACCACCCA CACCACCCTG
GAGCACTCTG ATTGTGCCTT CATGGTAGAC AATGAGGCCA TCTATGACAT CTGTCGTAGA
AACCTCGATA TTGAGCGTCC AACCTATACT AACCTGAATA GGTTAATAGG TCAAATTGTG
TCCTCCATCA CTGCTTCCCT GAGATTTGAT GGAGCCCTGA ATGTTGACCT GACAGAATTC
CAGACCAACC TGGTGCCCTA TCCCCGCATC CACTTCCCTC TGGCCACATA TGCCCCTGTC
ATCTCTGCTG AGAAAGCCTA CCATGAACAG CTTTCTGTAG CAGAGATCAC CAATGCTTGC
TTTGAGCCAG CCAACCAGAT GGTGAAATGT GACCCTCGCC ATGGTAAATA CATGGCTTGC
TGCCTGTTGT ACCGTGGTGA CGTGGTTCCC AAAGATGTCA ATGCTGCCAT TGCCACCATC
AAGACCAAGC GTACCATCCA GTTTGTGGAT TGGTGCCCCA CTGGCTTCAA GGTTGGCATC
AACTACCAGC CTCCCACTGT GGTGCCTGGT GGAGACCTGG CCAAGGTACA GAGAGCTGTG
TGCATGCTGA GCAACACCAC AGCCATTGCT GAGGCCTGGG CTCGCCTGGA CCACAAGTTT
GACCTGATGT ATGCCAAACG TGCCTTTGTT CACTGGTACG TTGGGGAGGG GATGGAGGAA
GGTGAGTTTT CAGAGGCCCG TGAGGACATG GCTGCCCTTG AGAAGGATTA TGAGGAGGTT
GGTGTGGATT CTGTTGAAGG AGAGGGTGAG GAAGAAGGAG AGGAATACTA A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001257329.1
CDS539..1789
Misc Feature(1)350..352(+)
Misc Feature(2)539..1753(+)
Misc Feature(3)539..1738(+)
Misc Feature(4)644..1117(+)
Misc Feature(5)647..1105(+)
Misc Feature(6)1028..1489(+)
Exon (1)1..436
Gene:TUBA1A
Gene Synonym:
Exon (2)437..659
Gene:TUBA1A
Gene Synonym:
Exon (3)660..808
Gene:TUBA1A
Gene Synonym:
Exon (4)809..2004
Gene:TUBA1A
Gene Synonym:
Translation
Position Chain Variation Link
14 14 c, g dbSNP:763523023
45 45 a, g dbSNP:753215197
63 63 a, g dbSNP:192252132
69 69 a, g dbSNP:765829618
86 86 a, g dbSNP:548167433
97 97 c, t dbSNP:531238602
110 110 c, g dbSNP:562588719
112 112 c, g dbSNP:187974148
158 158 c, t dbSNP:545598107
169 169 c, g dbSNP:532306297
222 222 a, g dbSNP:760023279
223 223 a, g dbSNP:559919161
224 224 a, c dbSNP:540076934
268 268 a, c, t dbSNP:574389302
272 272 g, t dbSNP:554470192
277 277 a, g dbSNP:371310839
289 289 a, g dbSNP:182661252
292 292 a, g dbSNP:11558063
294 294 -, aac dbSNP:751720598
302 302 -, ctc dbSNP:766250512
303 303 c, t dbSNP:767287078
304 304 c, t dbSNP:761622371
306 306 c, t dbSNP:773953696
307 307 a, c, t dbSNP:11558074
313 313 c, t dbSNP:762428394
316 316 c, t dbSNP:775124314
324 324 a, c dbSNP:770196685
325 325 c, g, t dbSNP:777015483
331 331 c, t dbSNP:192151179
340 340 a, g dbSNP:747406864
342 342 a, c dbSNP:539551108
351 351 a, g dbSNP:758738979
358 358 c, g dbSNP:748160639
360 360 c, t dbSNP:778988809
364 364 a, c, g dbSNP:750328538
366 366 c, t dbSNP:767387627
369 369 c, t dbSNP:757016133
371 371 c, t dbSNP:751404305
375 375 a, g dbSNP:763724020
377 377 c, t dbSNP:762660170
378 378 -, g dbSNP:146808943
378 378 g, t dbSNP:775008245
379 379 g, t dbSNP:764746345
393 393 a, c dbSNP:758990710
395 395 a, c dbSNP:774938419
406 406 c, t dbSNP:368699278
416 416 a, c dbSNP:1039225
425 425 c, t dbSNP:139383482
430 430 c, t dbSNP:747433806
433 433 c, t dbSNP:534443774
438 438 a, g dbSNP:587784491
442 442 a, g dbSNP:764828844
446 446 a, c dbSNP:387906840
457 457 c, t dbSNP:143047732
499 499 a, g dbSNP:375516850
502 502 c, g dbSNP:766977640
517 517 c, t dbSNP:761327879
529 529 c, t dbSNP:139102191
556 556 c, t dbSNP:200198307
565 565 a, t dbSNP:772518269
574 574 c, t dbSNP:762120179
576 576 a, c dbSNP:11558073
577 577 c, t dbSNP:372306943
585 585 c, t dbSNP:587784485
586 586 c, g dbSNP:11558072
595 595 a, t dbSNP:587784486
598 598 a, g dbSNP:1055904
604 604 g, t dbSNP:1055956
616 616 c, t dbSNP:774605003
622 622 c, t dbSNP:147273934
625 625 a, g dbSNP:749375580
679 679 c, t dbSNP:1134868
691 691 c, g dbSNP:775649551
706 706 a, g dbSNP:765483435
709 709 c, t dbSNP:1065671
709 709 c, t dbSNP:587784487
721 721 a, g dbSNP:1056875
742 742 c, t dbSNP:772038983
745 745 c, g dbSNP:747966442
763 763 c, t dbSNP:774370656
770 770 c, g dbSNP:769889742
781 781 c, t dbSNP:768297938
784 784 c, t dbSNP:749139359
787 787 a, g dbSNP:779834877
808 808 a, g dbSNP:755889624
811 811 c, t dbSNP:1135224
817 817 a, g dbSNP:201704429
820 820 c, t dbSNP:745462580
823 823 a, c, g dbSNP:1143559
824 824 a, g dbSNP:11558067
829 829 c, t dbSNP:1143560
841 841 a, g dbSNP:201587501
850 850 c, t dbSNP:11558068
859 859 c, t dbSNP:778119816
865 865 a, t dbSNP:758957354
871 871 g, t dbSNP:753052479
886 886 a, c, g dbSNP:697624
904 904 c, t dbSNP:753969793
914 914 g, t dbSNP:587784488
925 925 a, g dbSNP:566021671
937 937 a, g dbSNP:760653068
943 943 c, t dbSNP:112023543
949 949 c, t dbSNP:370227094
952 952 a, g dbSNP:200372205
954 954 c, t dbSNP:587784489
955 955 a, g dbSNP:61730859
958 958 a, c, t dbSNP:775583580
976 976 a, c dbSNP:544713824
979 979 a, t dbSNP:759405602
995 995 a, c dbSNP:137853045
997 997 -, atc dbSNP:587784490
998 998 c, t dbSNP:113208258
1012 1012 c, t dbSNP:140239444
1021 1021 a, g dbSNP:770665177
1024 1024 c, t dbSNP:746486977
1029 1029 a, g dbSNP:786205479
1063 1063 c, t dbSNP:777403500
1075 1075 c, t dbSNP:771571589
1081 1081 c, t dbSNP:748593535
1084 1084 c, t dbSNP:779416899
1090 1090 c, t dbSNP:146680997
1096 1096 c, t dbSNP:754280693
1108 1108 c, t dbSNP:780129528
1120 1120 a, g dbSNP:756428949
1131 1131 a, g dbSNP:587784492
1144 1144 c, t dbSNP:267603493
1191 1191 c, g dbSNP:587784493
1207 1207 c, t dbSNP:750614684
1213 1213 a, g dbSNP:767648261
1219 1219 c, t dbSNP:762008241
1220 1220 a, c dbSNP:137853046
1223 1223 c, t dbSNP:137853043
1240 1240 a, g dbSNP:752640192
1241 1241 g, t dbSNP:587784494
1252 1252 c, t dbSNP:140795162
1254 1254 -, c, ct dbSNP:34268631
1282 1282 c, t dbSNP:759228609
1292 1292 a, t dbSNP:150978489
1351 1351 c, t dbSNP:765938699
1360 1360 c, t dbSNP:760304736
1384 1384 c, g dbSNP:565144370
1385 1385 c, t dbSNP:771520376
1387 1387 a, g dbSNP:545234429
1399 1399 c, t dbSNP:142641191
1419 1419 a, g dbSNP:587784495
1423 1423 c, t dbSNP:774821065
1424 1424 a, g dbSNP:587784496
1426 1426 c, g dbSNP:769212646
1428 1428 c, t dbSNP:587784497
1429 1429 c, t dbSNP:148479477
1441 1441 a, g dbSNP:11829764
1477 1477 c, t dbSNP:144574983
1498 1498 c, t dbSNP:746054164
1501 1501 c, t dbSNP:781597673
1528 1528 c, t dbSNP:140737357
1546 1546 -, ta dbSNP:200449362
1561 1561 c, t dbSNP:751737187
1562 1562 a, g dbSNP:587784481
1581 1581 c, t dbSNP:587784482
1600 1600 c, t dbSNP:199813289
1623 1623 c, t dbSNP:137853048
1636 1636 a, g dbSNP:1065724
1637 1637 a, c, t dbSNP:587784483
1638 1638 a, g, t dbSNP:137853044
1657 1657 c, t dbSNP:753719501
1660 1660 g, t dbSNP:766135902
1663 1663 c, g, t dbSNP:772809398
1681 1681 c, t dbSNP:3206694
1687 1687 c, t dbSNP:201884190
1689 1689 c, t dbSNP:137853047
1691 1691 a, g dbSNP:11558065
1696 1696 c, g dbSNP:11558070
1697 1697 c, t dbSNP:137853049
1698 1698 a, g dbSNP:137853050
1699 1699 g, t dbSNP:761301881
1707 1707 a, t dbSNP:587784484
1713 1713 c, g dbSNP:773920941
1732 1732 a, g dbSNP:768060474
1751 1751 a, g dbSNP:749746325
1753 1753 c, t dbSNP:11558069
1762 1762 a, g dbSNP:775885354
1764 1764 c, g dbSNP:770156445
1768 1768 a, g dbSNP:746276826
1772 1772 a, g dbSNP:1065730
1780 1780 a, g dbSNP:200873943
1790 1790 -, a dbSNP:781483247
1793 1793 -, t dbSNP:768839661
1798 1798 c, t dbSNP:371718931
1799 1799 a, g dbSNP:747080229
1802 1802 a, c dbSNP:1062437
1805 1805 a, g dbSNP:777929464
1808 1808 a, g dbSNP:758417587
1811 1811 c, t dbSNP:753757160
1812 1812 c, t dbSNP:766255841
1815 1815 g, t dbSNP:185938303
1876 1876 a, g dbSNP:11558066
1895 1895 c, t dbSNP:1062439
1902 1902 a, g dbSNP:556755463
1903 1903 c, t dbSNP:150293856
1908 1908 -, a dbSNP:757230901
1913 1913 c, t dbSNP:571426354
1921 1921 c, t dbSNP:1062440
1935 1935 c, t dbSNP:557975791
1963 1963 c, t dbSNP:1062441
1991 1991 -, ct dbSNP:753859336
2000 2000 a, g dbSNP:140121590

Target ORF information:

RefSeq Version NM_001270400
Organism Homo sapiens (human)
Definition Homo sapiens tubulin, alpha 1a (TUBA1A), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001270400

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCAAGTG ACAAGACCAT TGGGGGAGGA GATGATTCCT TCAACACCTT CTTCAGTGAG 
ACGGGGGCTG GCAAGCATGT GCCCCGGGCA GTGTTTGTAG ACTTGGAACC CACAGTCATT
GATGAAGTTC GCACTGGCAC CTACCGCCAG CTCTTCCACC CTGAGCAACT TATCACAGGC
AAAGAAGATG CTGCCAATAA CTATGCCCGA GGGCACTACA CCATTGGCAA GGAGATCATT
GACCTCGTGT TGGACCGAAT TCGCAAGCTG GCCGACCAGT GCACGGGTCT CCAGGGCTTC
TTGGTTTTCC ACAGCTTTGG TGGGGGAACT GGTTCTGGGT TCACCTCGCT GCTCATGGAA
CGTCTCTCAG TTGATTATGG CAAGAAGTCC AAGCTGGAGT TCTCTATTTA CCCGGCGCCC
CAGGTTTCCA CAGCTGTAGT TGAGCCCTAC AACTCCATCC TCACCACCCA CACCACCCTG
GAGCACTCTG ATTGTGCCTT CATGGTAGAC AATGAGGCCA TCTATGACAT CTGTCGTAGA
AACCTCGATA TTGAGCGTCC AACCTATACT AACCTGAATA GGTTAATAGG TCAAATTGTG
TCCTCCATCA CTGCTTCCCT GAGATTTGAT GGAGCCCTGA ATGTTGACCT GACAGAATTC
CAGACCAACC TGGTGCCCTA TCCCCGCATC CACTTCCCTC TGGCCACATA TGCCCCTGTC
ATCTCTGCTG AGAAAGCCTA CCATGAACAG CTTTCTGTAG CAGAGATCAC CAATGCTTGC
TTTGAGCCAG CCAACCAGAT GGTGAAATGT GACCCTCGCC ATGGTAAATA CATGGCTTGC
TGCCTGTTGT ACCGTGGTGA CGTGGTTCCC AAAGATGTCA ATGCTGCCAT TGCCACCATC
AAGACCAAGC GTACCATCCA GTTTGTGGAT TGGTGCCCCA CTGGCTTCAA GGTTGGCATC
AACTACCAGC CTCCCACTGT GGTGCCTGGT GGAGACCTGG CCAAGGTACA GAGAGCTGTG
TGCATGCTGA GCAACACCAC AGCCATTGCT GAGGCCTGGG CTCGCCTGGA CCACAAGTTT
GACCTGATGT ATGCCAAACG TGCCTTTGTT CACTGGTACG TTGGGGAGGG GATGGAGGAA
GGTGAGTTTT CAGAGGCCCG TGAGGACATG GCTGCCCTTG AGAAGGATTA TGAGGAGGTT
GGTGTGGATT CTGTTGAAGG AGAGGGTGAG GAAGAAGGAG AGGAATACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.