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FA2H fatty acid 2-hydroxylase [Homo sapiens (human)]

Gene Symbol FA2H
Entrez Gene ID 79152
Full Name fatty acid 2-hydroxylase
Synonyms FAAH, FAH1, FAXDC1, SCS7, SPG35
General protein information
Preferred Names
fatty acid 2-hydroxylase
Names
fatty acid 2-hydroxylase
fatty acid alpha-hydroxylase
fatty acid hydroxylase domain containing 1
spastic paraplegia 35 (autosomal recessive)
NP_077282.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16q23

Summary This gene encodes a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups. Mutations in this gene have been associated with leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia.[provided by RefSeq, Mar 2010]. lac of sum
Disorder MIM:

611026

Disorder Html: Leukodystrophy, dysmyelinating, and spastic paraparesis with or
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu71802 XM_011523317 PREDICTED: Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK Not in stock -1 Starting from $99.00
OHu71803 XM_011523318 PREDICTED: Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK Not in stock -1 Starting from $99.00
OHu71804 XM_011523319 PREDICTED: Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu26599 NM_024306 Homo sapiens fatty acid 2-hydroxylase (FA2H), mRNA. pcDNA3.1-C-(k)DYK In stock -1 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu71802D
Sequence Information ORF Nucleotide Sequence (Length: 1110bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product fatty acid 2-hydroxylase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC
CACAAGAGCA GGAGACTGAG GCCTAGGAGG ACTAAGGAAC TTGCCCCAGG TCACACTTGG
CACAGCCAGG ATGTCGTACC CCATTCTGGG GATTCCAGAG TCTGCGCTCT CAATCACCCT
TCCCCTGAGG CCCTTCAAGG ACTCCACGAC AGTCCCAGAA GCTGGCCCGA GAAGATAGGT
CTGCCCAAAG GAAGCTACCT CCCCTGTACC CCCCGCTGCT TCTCTGTCCC TGCATTGTGC
TGTCCTTGGC CCATCCCAGG CTGTTCCCCA GCCCGTCTCC ACACTCTGTT CTCTGCTGGG
CATAAATCAG AGAAAATATG GCAGATATGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011521619.1
CDS69..1178
Misc Feature(1)111..323(+)
Misc Feature(2)438..>857(+)
Translation
Position Chain Variation Link
36 36 c, t dbSNP:569711094
50 50 a, g dbSNP:750069577
74 74 c, t dbSNP:113471134
84 84 c, t dbSNP:773739894
94 94 c, g dbSNP:12930201
97 97 c, t dbSNP:12930196
123 123 c, t dbSNP:763556766
139 139 c, g dbSNP:762615677
150 150 c, t dbSNP:775172785
153 153 c, g dbSNP:550064357
155 155 c, g dbSNP:746288688
171 171 g, t dbSNP:121918217
182 182 c, t dbSNP:777043040
186 186 c, g dbSNP:771357835
190 190 a, g dbSNP:747507656
230 230 a, g dbSNP:778018677
246 246 a, g dbSNP:533048648
252 252 c, t dbSNP:748204186
263 263 a, g dbSNP:779343147
266 266 c, g dbSNP:570454174
270 270 a, g dbSNP:750198250
297 297 c, t dbSNP:929881
300 300 a, g dbSNP:527421775
314 314 a, g dbSNP:751449227
327 327 g, t dbSNP:561760287
332 332 a, g dbSNP:541909354
348 348 a, g dbSNP:761184809
349 349 a, t dbSNP:267604636
351 351 a, g dbSNP:773696633
353 353 c, t dbSNP:772718168
354 354 a, g dbSNP:762343484
357 357 c, g dbSNP:35874850
360 360 a, g dbSNP:768732810
403 403 c, t dbSNP:749305581
405 405 c, t dbSNP:141276237
406 406 a, g dbSNP:147632811
414 414 a, g dbSNP:746868199
415 415 a, t dbSNP:777488679
416 416 c, g dbSNP:758340506
418 418 a, c, t dbSNP:145990810
422 422 c, t dbSNP:778689972
434 434 c, g dbSNP:757737719
435 435 c, t dbSNP:751867997
443 443 a, c dbSNP:764721748
455 455 g, t dbSNP:561163080
467 467 a, g dbSNP:763314788
469 469 g, t dbSNP:199659429
469 469 -, t dbSNP:777658535
472 472 a, g dbSNP:765264675
473 473 a, c, g dbSNP:776875732
474 474 a, c dbSNP:771134202
475 475 a, c dbSNP:761723049
478 478 g, t dbSNP:773958471
479 479 a, g, t dbSNP:749140634
491 491 c, t dbSNP:201250822
492 492 a, g dbSNP:769477853
494 494 c, t dbSNP:142676543
498 498 g, t dbSNP:781059993
501 501 a, g, t dbSNP:751969460
503 503 c, t dbSNP:777935324
505 505 a, c, g dbSNP:201162733
511 511 c, t dbSNP:372350326
512 512 a, g, t dbSNP:138087787
519 519 a, g dbSNP:753898846
524 524 c, t dbSNP:766599125
528 528 c, t dbSNP:387907040
531 531 c, t dbSNP:774231502
539 539 c, g dbSNP:146060468
544 544 a, g, t dbSNP:149561762
547 547 a, t dbSNP:138240770
548 548 c, t dbSNP:775608310
554 554 c, g dbSNP:371859188
555 555 a, g dbSNP:745430083
558 558 c, g, t dbSNP:770624203
565 565 a, c dbSNP:746605230
571 571 c, t dbSNP:778226239
572 572 c, g dbSNP:758742229
578 578 -, ca dbSNP:587776891
597 597 a, g dbSNP:201500782
599 599 a, g dbSNP:775888031
600 600 c, t dbSNP:749744896
605 605 a, g dbSNP:75711361
608 608 g, t dbSNP:150423523
611 611 a, g dbSNP:750511573
617 617 c, t dbSNP:767690383
619 619 g, t dbSNP:757444137
622 622 c, g dbSNP:752546362
633 633 c, t dbSNP:765086319
634 634 a, g dbSNP:370682047
638 638 a, c dbSNP:138892784
641 641 c, t dbSNP:766313852
654 654 a, g, t dbSNP:565757665
658 658 a, g dbSNP:771625273
667 667 c, t dbSNP:139261750
668 668 a, g dbSNP:774718977
676 676 c, g, t dbSNP:749620420
688 688 c, t dbSNP:372445274
689 689 g, t dbSNP:779856022
692 692 a, g dbSNP:756025962
696 696 g, t dbSNP:541338991
698 698 a, g dbSNP:767410582
701 701 c, t dbSNP:761379296
702 702 a, g dbSNP:751056475
703 703 a, c dbSNP:759577964
716 716 c, g, t dbSNP:762526718
717 717 a, g dbSNP:775750642
722 722 c, g dbSNP:770149919
737 737 a, c dbSNP:369504312
740 740 a, c dbSNP:376180814
741 741 c, t dbSNP:771564922
743 743 c, g, t dbSNP:371803211
748 748 c, g dbSNP:772449065
755 755 c, t dbSNP:748364155
770 770 c, t dbSNP:778973971
771 771 c, t dbSNP:387907039
772 772 a, g dbSNP:572112273
775 775 c, t dbSNP:387907172
776 776 c, g, t dbSNP:781172621
777 777 c, g dbSNP:757119964
786 786 a, g dbSNP:367933620
792 792 a, c dbSNP:763544424
793 793 c, t dbSNP:758016356
800 800 c, t dbSNP:142632840
801 801 a, g dbSNP:201126702
808 808 a, g dbSNP:760000001
809 809 c, t dbSNP:776851677
818 818 c, g dbSNP:146684287
822 822 c, g, t dbSNP:761217557
831 831 a, g dbSNP:773459488
836 836 a, g dbSNP:772321218
839 839 c, t dbSNP:371293493
840 840 a, g dbSNP:774693133
841 841 a, g dbSNP:768757432
850 850 a, g dbSNP:761645282
852 852 a, g dbSNP:780906296
853 853 a, c dbSNP:555874983
863 863 a, t dbSNP:757971735
867 867 a, g dbSNP:373086169
872 872 g, t dbSNP:749879689
874 874 c, g dbSNP:778686303
888 888 c, g dbSNP:184043230
914 914 c, t dbSNP:763535821
923 923 c, t dbSNP:753423291
924 924 a, g dbSNP:760210740
928 928 c, g dbSNP:193234107
954 954 a, g dbSNP:760412438
967 967 c, t dbSNP:752710721
996 996 a, g dbSNP:545536672
1010 1010 c, t dbSNP:115573140
1016 1016 c, g dbSNP:60833066
1060 1060 c, g dbSNP:540206601
1061 1061 c, g dbSNP:188570108
1062 1062 c, t dbSNP:573535704
1077 1077 c, t dbSNP:556067560
1097 1097 g, t dbSNP:767326341
1101 1101 a, g dbSNP:8047571
1111 1111 a, g dbSNP:576668065
1116 1116 c, g dbSNP:34130324
1122 1122 c, t dbSNP:376224461
1130 1130 a, c dbSNP:183019164
1132 1132 c, t dbSNP:190488301
1134 1134 -, ct dbSNP:558386529
1134 1134 c, g dbSNP:541673499
1150 1150 a, g dbSNP:8047410
1155 1155 c, t dbSNP:75432008
1157 1157 a, g dbSNP:370145963
1167 1167 g, t dbSNP:568133353
1184 1184 c, t dbSNP:77381824
1185 1185 c, t dbSNP:531507806
1211 1211 c, t dbSNP:747308512
1212 1212 a, g dbSNP:569101661
1224 1224 c, t dbSNP:185776418
1225 1225 a, g dbSNP:532238867
1238 1238 c, t dbSNP:373276252
1263 1263 a, g dbSNP:560169715
1285 1285 c, t dbSNP:778293305
1299 1299 a, c dbSNP:539973891
1327 1327 a, g dbSNP:756697337
1350 1350 -, c dbSNP:568677351
1383 1383 a, g dbSNP:182540053
1400 1400 c, g dbSNP:560877025
1401 1401 c, g dbSNP:542493611
1433 1433 g, t dbSNP:753509087
1451 1451 c, t dbSNP:773458399
1452 1452 c, g dbSNP:576800387
1499 1499 c, g dbSNP:73614661
1546 1546 a, t dbSNP:190907945
1573 1573 c, t dbSNP:577390048
1574 1574 a, g dbSNP:554206979
1580 1580 a, t dbSNP:555574452
1598 1598 c, t dbSNP:76400275
1620 1620 c, g dbSNP:529961573
1637 1637 a, g dbSNP:139106794
1690 1690 g, t dbSNP:554543586
1706 1706 c, g dbSNP:537887205
1721 1721 c, t dbSNP:569115335
1747 1747 c, t dbSNP:566769506
1748 1748 a, g dbSNP:538796394
1762 1762 a, g dbSNP:566562243
1765 1765 a, g dbSNP:185986886
1791 1791 c, t dbSNP:118054363
1794 1794 a, c, t dbSNP:73614660
1806 1806 c, g dbSNP:550580438
1821 1821 c, t dbSNP:530550098
1829 1829 -, g dbSNP:745742563
1847 1847 a, g dbSNP:77737883
1852 1852 a, c dbSNP:571171508
1896 1896 a, g dbSNP:34089769
1898 1898 c, t dbSNP:116207483
1923 1923 c, t dbSNP:73614659
1932 1932 a, g dbSNP:182322987
1965 1965 c, t dbSNP:568962315
2045 2045 c, t dbSNP:34219496
2048 2048 c, g dbSNP:150744043
2053 2053 c, g dbSNP:559238763
2067 2067 c, t dbSNP:558781824
2074 2074 g, t dbSNP:538564860
2094 2094 a, c dbSNP:35937344
2100 2100 c, t dbSNP:79621652
2142 2142 a, c dbSNP:536486449
2159 2159 c, t dbSNP:567348711
2228 2228 c, t dbSNP:144035021
2230 2230 c, t dbSNP:770455797
2231 2231 a, g dbSNP:138484070
2235 2235 a, g dbSNP:777447527
2239 2239 c, t dbSNP:530493414
2248 2248 a, g dbSNP:755708669
2255 2255 c, t dbSNP:533676992
2271 2271 a, g dbSNP:147497108
2278 2278 c, t dbSNP:780852049
2309 2309 a, c dbSNP:754742051
2344 2344 a, g dbSNP:190592437
2354 2354 c, g dbSNP:115964510
2377 2377 a, g dbSNP:186096958
2379 2379 cc, tt dbSNP:386792122
2379 2379 c, t dbSNP:114912635
2380 2380 c, t dbSNP:76223019
2390 2390 a, g dbSNP:561017577
2408 2408 -, aca dbSNP:769055927
2415 2415 a, g dbSNP:544313519
2452 2452 c, t dbSNP:73614655
2464 2464 c, t dbSNP:760149970
2467 2467 c, t dbSNP:558682841
2522 2522 c, g dbSNP:545431686
2544 2544 c, t dbSNP:143338264
2547 2547 c, t dbSNP:555862244
2584 2584 c, t dbSNP:113223277
2588 2588 c, t dbSNP:181560628
2596 2596 a, g dbSNP:113713735
2605 2605 c, t dbSNP:760815533
2620 2620 c, g dbSNP:775725816
2640 2640 a, g dbSNP:56664536
2642 2642 a, g dbSNP:557055136
2671 2671 a, g dbSNP:112754063
2680 2680 c, t dbSNP:571370504
2720 2720 c, t dbSNP:766896095
2733 2733 c, t dbSNP:143255996
2740 2740 a, g dbSNP:528121279
2742 2742 c, t dbSNP:747693437
2760 2760 c, g dbSNP:778242149
2762 2762 c, g dbSNP:754694789
2764 2764 c, t dbSNP:370734744
2770 2770 c, t dbSNP:57844314
2774 2774 c, g dbSNP:779833110
2775 2775 c, g dbSNP:773369721
2784 2784 a, g dbSNP:374680337
2793 2793 c, g dbSNP:750814402
2815 2815 c, t dbSNP:530195358

Target ORF information:

RefSeq Version XM_011523317
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011523317

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC
CACAAGAGCA GGAGACTGAG GCCTAGGAGG ACTAAGGAAC TTGCCCCAGG TCACACTTGG
CACAGCCAGG ATGTCGTACC CCATTCTGGG GATTCCAGAG TCTGCGCTCT CAATCACCCT
TCCCCTGAGG CCCTTCAAGG ACTCCACGAC AGTCCCAGAA GCTGGCCCGA GAAGATAGGT
CTGCCCAAAG GAAGCTACCT CCCCTGTACC CCCCGCTGCT TCTCTGTCCC TGCATTGTGC
TGTCCTTGGC CCATCCCAGG CTGTTCCCCA GCCCGTCTCC ACACTCTGTT CTCTGCTGGG
CATAAATCAG AGAAAATATG GCAGATATGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu71803D
Sequence Information ORF Nucleotide Sequence (Length: 969bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product fatty acid 2-hydroxylase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC
CACAAGAGCA GGAGACTGAG GCCTAGGAGG ACTAAGGAAC TTGCCCCAGG TCACACTTGG
CACAGCCAGG ATGTCGTACC CCATTCTGGG GATTCCAGAG TCTGCGCTCT CAATCACCCT
TCCCCTGAGG CCCTTCAAGG ACTCCACGAC AGTCCCAGAA GCTGGCCCGA GAAGATAGCA
GCAACTTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011521620.1
CDS69..1037
Misc Feature(1)111..323(+)
Misc Feature(2)438..>857(+)
Translation
Position Chain Variation Link
36 36 c, t dbSNP:569711094
50 50 a, g dbSNP:750069577
74 74 c, t dbSNP:113471134
84 84 c, t dbSNP:773739894
94 94 c, g dbSNP:12930201
97 97 c, t dbSNP:12930196
123 123 c, t dbSNP:763556766
139 139 c, g dbSNP:762615677
150 150 c, t dbSNP:775172785
153 153 c, g dbSNP:550064357
155 155 c, g dbSNP:746288688
171 171 g, t dbSNP:121918217
182 182 c, t dbSNP:777043040
186 186 c, g dbSNP:771357835
190 190 a, g dbSNP:747507656
230 230 a, g dbSNP:778018677
246 246 a, g dbSNP:533048648
252 252 c, t dbSNP:748204186
263 263 a, g dbSNP:779343147
266 266 c, g dbSNP:570454174
270 270 a, g dbSNP:750198250
297 297 c, t dbSNP:929881
300 300 a, g dbSNP:527421775
314 314 a, g dbSNP:751449227
327 327 g, t dbSNP:561760287
332 332 a, g dbSNP:541909354
348 348 a, g dbSNP:761184809
349 349 a, t dbSNP:267604636
351 351 a, g dbSNP:773696633
353 353 c, t dbSNP:772718168
354 354 a, g dbSNP:762343484
357 357 c, g dbSNP:35874850
360 360 a, g dbSNP:768732810
403 403 c, t dbSNP:749305581
405 405 c, t dbSNP:141276237
406 406 a, g dbSNP:147632811
414 414 a, g dbSNP:746868199
415 415 a, t dbSNP:777488679
416 416 c, g dbSNP:758340506
418 418 a, c, t dbSNP:145990810
422 422 c, t dbSNP:778689972
434 434 c, g dbSNP:757737719
435 435 c, t dbSNP:751867997
443 443 a, c dbSNP:764721748
455 455 g, t dbSNP:561163080
467 467 a, g dbSNP:763314788
469 469 g, t dbSNP:199659429
469 469 -, t dbSNP:777658535
472 472 a, g dbSNP:765264675
473 473 a, c, g dbSNP:776875732
474 474 a, c dbSNP:771134202
475 475 a, c dbSNP:761723049
478 478 g, t dbSNP:773958471
479 479 a, g, t dbSNP:749140634
491 491 c, t dbSNP:201250822
492 492 a, g dbSNP:769477853
494 494 c, t dbSNP:142676543
498 498 g, t dbSNP:781059993
501 501 a, g, t dbSNP:751969460
503 503 c, t dbSNP:777935324
505 505 a, c, g dbSNP:201162733
511 511 c, t dbSNP:372350326
512 512 a, g, t dbSNP:138087787
519 519 a, g dbSNP:753898846
524 524 c, t dbSNP:766599125
528 528 c, t dbSNP:387907040
531 531 c, t dbSNP:774231502
539 539 c, g dbSNP:146060468
544 544 a, g, t dbSNP:149561762
547 547 a, t dbSNP:138240770
548 548 c, t dbSNP:775608310
554 554 c, g dbSNP:371859188
555 555 a, g dbSNP:745430083
558 558 c, g, t dbSNP:770624203
565 565 a, c dbSNP:746605230
571 571 c, t dbSNP:778226239
572 572 c, g dbSNP:758742229
578 578 -, ca dbSNP:587776891
597 597 a, g dbSNP:201500782
599 599 a, g dbSNP:775888031
600 600 c, t dbSNP:749744896
605 605 a, g dbSNP:75711361
608 608 g, t dbSNP:150423523
611 611 a, g dbSNP:750511573
617 617 c, t dbSNP:767690383
619 619 g, t dbSNP:757444137
622 622 c, g dbSNP:752546362
633 633 c, t dbSNP:765086319
634 634 a, g dbSNP:370682047
638 638 a, c dbSNP:138892784
641 641 c, t dbSNP:766313852
654 654 a, g, t dbSNP:565757665
658 658 a, g dbSNP:771625273
667 667 c, t dbSNP:139261750
668 668 a, g dbSNP:774718977
676 676 c, g, t dbSNP:749620420
688 688 c, t dbSNP:372445274
689 689 g, t dbSNP:779856022
692 692 a, g dbSNP:756025962
696 696 g, t dbSNP:541338991
698 698 a, g dbSNP:767410582
701 701 c, t dbSNP:761379296
702 702 a, g dbSNP:751056475
703 703 a, c dbSNP:759577964
716 716 c, g, t dbSNP:762526718
717 717 a, g dbSNP:775750642
722 722 c, g dbSNP:770149919
737 737 a, c dbSNP:369504312
740 740 a, c dbSNP:376180814
741 741 c, t dbSNP:771564922
743 743 c, g, t dbSNP:371803211
748 748 c, g dbSNP:772449065
755 755 c, t dbSNP:748364155
770 770 c, t dbSNP:778973971
771 771 c, t dbSNP:387907039
772 772 a, g dbSNP:572112273
775 775 c, t dbSNP:387907172
776 776 c, g, t dbSNP:781172621
777 777 c, g dbSNP:757119964
786 786 a, g dbSNP:367933620
792 792 a, c dbSNP:763544424
793 793 c, t dbSNP:758016356
800 800 c, t dbSNP:142632840
801 801 a, g dbSNP:201126702
808 808 a, g dbSNP:760000001
809 809 c, t dbSNP:776851677
818 818 c, g dbSNP:146684287
822 822 c, g, t dbSNP:761217557
831 831 a, g dbSNP:773459488
836 836 a, g dbSNP:772321218
839 839 c, t dbSNP:371293493
840 840 a, g dbSNP:774693133
841 841 a, g dbSNP:768757432
850 850 a, g dbSNP:761645282
852 852 a, g dbSNP:780906296
853 853 a, c dbSNP:555874983
863 863 a, t dbSNP:757971735
867 867 a, g dbSNP:373086169
872 872 g, t dbSNP:749879689
874 874 c, g dbSNP:778686303
888 888 c, g dbSNP:184043230
914 914 c, t dbSNP:763535821
923 923 c, t dbSNP:753423291
924 924 a, g dbSNP:760210740
928 928 c, g dbSNP:193234107
954 954 a, g dbSNP:760412438
967 967 c, t dbSNP:752710721
996 996 a, g dbSNP:545536672
1010 1010 c, t dbSNP:115573140
1016 1016 c, g dbSNP:60833066
1041 1041 a, g dbSNP:560169715
1063 1063 c, t dbSNP:778293305
1077 1077 a, c dbSNP:539973891
1105 1105 a, g dbSNP:756697337
1128 1128 -, c dbSNP:568677351
1161 1161 a, g dbSNP:182540053
1178 1178 c, g dbSNP:560877025
1179 1179 c, g dbSNP:542493611
1211 1211 g, t dbSNP:753509087
1229 1229 c, t dbSNP:773458399
1230 1230 c, g dbSNP:576800387
1277 1277 c, g dbSNP:73614661
1324 1324 a, t dbSNP:190907945
1351 1351 c, t dbSNP:577390048
1352 1352 a, g dbSNP:554206979
1358 1358 a, t dbSNP:555574452
1376 1376 c, t dbSNP:76400275
1398 1398 c, g dbSNP:529961573
1415 1415 a, g dbSNP:139106794
1468 1468 g, t dbSNP:554543586
1484 1484 c, g dbSNP:537887205
1499 1499 c, t dbSNP:569115335
1525 1525 c, t dbSNP:566769506
1526 1526 a, g dbSNP:538796394
1540 1540 a, g dbSNP:566562243
1543 1543 a, g dbSNP:185986886
1569 1569 c, t dbSNP:118054363
1572 1572 a, c, t dbSNP:73614660
1584 1584 c, g dbSNP:550580438
1599 1599 c, t dbSNP:530550098
1607 1607 -, g dbSNP:745742563
1625 1625 a, g dbSNP:77737883
1630 1630 a, c dbSNP:571171508
1674 1674 a, g dbSNP:34089769
1676 1676 c, t dbSNP:116207483
1701 1701 c, t dbSNP:73614659
1710 1710 a, g dbSNP:182322987
1743 1743 c, t dbSNP:568962315
1823 1823 c, t dbSNP:34219496
1826 1826 c, g dbSNP:150744043
1831 1831 c, g dbSNP:559238763
1845 1845 c, t dbSNP:558781824
1852 1852 g, t dbSNP:538564860
1872 1872 a, c dbSNP:35937344
1878 1878 c, t dbSNP:79621652
1920 1920 a, c dbSNP:536486449
1937 1937 c, t dbSNP:567348711
2006 2006 c, t dbSNP:144035021
2008 2008 c, t dbSNP:770455797
2009 2009 a, g dbSNP:138484070
2013 2013 a, g dbSNP:777447527
2017 2017 c, t dbSNP:530493414
2026 2026 a, g dbSNP:755708669
2033 2033 c, t dbSNP:533676992
2049 2049 a, g dbSNP:147497108
2056 2056 c, t dbSNP:780852049
2087 2087 a, c dbSNP:754742051
2122 2122 a, g dbSNP:190592437
2132 2132 c, g dbSNP:115964510
2155 2155 a, g dbSNP:186096958
2157 2157 cc, tt dbSNP:386792122
2157 2157 c, t dbSNP:114912635
2158 2158 c, t dbSNP:76223019
2168 2168 a, g dbSNP:561017577
2186 2186 -, aca dbSNP:769055927
2193 2193 a, g dbSNP:544313519
2230 2230 c, t dbSNP:73614655
2242 2242 c, t dbSNP:760149970
2245 2245 c, t dbSNP:558682841
2300 2300 c, g dbSNP:545431686
2322 2322 c, t dbSNP:143338264
2325 2325 c, t dbSNP:555862244
2362 2362 c, t dbSNP:113223277
2366 2366 c, t dbSNP:181560628
2374 2374 a, g dbSNP:113713735
2383 2383 c, t dbSNP:760815533
2398 2398 c, g dbSNP:775725816
2418 2418 a, g dbSNP:56664536
2420 2420 a, g dbSNP:557055136
2449 2449 a, g dbSNP:112754063
2458 2458 c, t dbSNP:571370504
2498 2498 c, t dbSNP:766896095
2511 2511 c, t dbSNP:143255996
2518 2518 a, g dbSNP:528121279
2520 2520 c, t dbSNP:747693437
2538 2538 c, g dbSNP:778242149
2540 2540 c, g dbSNP:754694789
2542 2542 c, t dbSNP:370734744
2548 2548 c, t dbSNP:57844314
2552 2552 c, g dbSNP:779833110
2553 2553 c, g dbSNP:773369721
2562 2562 a, g dbSNP:374680337
2571 2571 c, g dbSNP:750814402
2593 2593 c, t dbSNP:530195358

Target ORF information:

RefSeq Version XM_011523318
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011523318

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC
CACAAGAGCA GGAGACTGAG GCCTAGGAGG ACTAAGGAAC TTGCCCCAGG TCACACTTGG
CACAGCCAGG ATGTCGTACC CCATTCTGGG GATTCCAGAG TCTGCGCTCT CAATCACCCT
TCCCCTGAGG CCCTTCAAGG ACTCCACGAC AGTCCCAGAA GCTGGCCCGA GAAGATAGCA
GCAACTTAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu71804D
Sequence Information ORF Nucleotide Sequence (Length: 879bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product fatty acid 2-hydroxylase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGATGGTG TTCGCCTGTG GAATTCAAGG GGCTCCATGG AGAACGAGCC TGTAGCCCTT 
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC
CACAAGGCAC CCTTCGACGG CTCCCGCCTG GTCTTCCCCC CTGTGCCAGC CTCCCTGGTG
ATCGGCGTCT TCTACTTGTG CATGCAGCTC ATCCTGCCCG AGGCAGTAGG GGGCACTGTG
TTTGCGGGGG GCCTCCTGGG CTACGTCCTC TATGACATGA CCCATTACTA CCTGCACTTT
GGCTCGCCGC ACAAGGGCTC CTACCTGTAC AGCCTGAAGG CCCACCACGT CAAGCACCAC
TTTGCACATC AGAAGTCAGG ATTTGGTATC AGCACTAAAT TGTGGGATTA CTGTTTCCAC
ACCCTCACTC CAGAGAAACC CCACCTGAAG ACGCAGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011521621.1
CDS46..924
Misc Feature(1)175..903(+)
Misc Feature(2)433..906(+)
Translation
Position Chain Variation Link
1 1 g, t dbSNP:78256860
12 12 g, t dbSNP:777514584
15 15 c, t dbSNP:553751619
37 37 g, t dbSNP:755981569
59 59 c, g dbSNP:188415528
75 75 a, g dbSNP:183698497
85 85 a, g dbSNP:761184809
86 86 a, t dbSNP:267604636
88 88 a, g dbSNP:773696633
90 90 c, t dbSNP:772718168
91 91 a, g dbSNP:762343484
94 94 c, g dbSNP:35874850
97 97 a, g dbSNP:768732810
140 140 c, t dbSNP:749305581
142 142 c, t dbSNP:141276237
143 143 a, g dbSNP:147632811
151 151 a, g dbSNP:746868199
152 152 a, t dbSNP:777488679
153 153 c, g dbSNP:758340506
155 155 a, c, t dbSNP:145990810
159 159 c, t dbSNP:778689972
171 171 c, g dbSNP:757737719
172 172 c, t dbSNP:751867997
180 180 a, c dbSNP:764721748
192 192 g, t dbSNP:561163080
204 204 a, g dbSNP:763314788
206 206 g, t dbSNP:199659429
206 206 -, t dbSNP:777658535
209 209 a, g dbSNP:765264675
210 210 a, c, g dbSNP:776875732
211 211 a, c dbSNP:771134202
212 212 a, c dbSNP:761723049
215 215 g, t dbSNP:773958471
216 216 a, g, t dbSNP:749140634
228 228 c, t dbSNP:201250822
229 229 a, g dbSNP:769477853
231 231 c, t dbSNP:142676543
235 235 g, t dbSNP:781059993
238 238 a, g, t dbSNP:751969460
240 240 c, t dbSNP:777935324
242 242 a, c, g dbSNP:201162733
248 248 c, t dbSNP:372350326
249 249 a, g, t dbSNP:138087787
256 256 a, g dbSNP:753898846
261 261 c, t dbSNP:766599125
265 265 c, t dbSNP:387907040
268 268 c, t dbSNP:774231502
276 276 c, g dbSNP:146060468
281 281 a, g, t dbSNP:149561762
284 284 a, t dbSNP:138240770
285 285 c, t dbSNP:775608310
291 291 c, g dbSNP:371859188
292 292 a, g dbSNP:745430083
295 295 c, g, t dbSNP:770624203
302 302 a, c dbSNP:746605230
308 308 c, t dbSNP:778226239
309 309 c, g dbSNP:758742229
315 315 -, ca dbSNP:587776891
334 334 a, g dbSNP:201500782
336 336 a, g dbSNP:775888031
337 337 c, t dbSNP:749744896
342 342 a, g dbSNP:75711361
345 345 g, t dbSNP:150423523
348 348 a, g dbSNP:750511573
354 354 c, t dbSNP:767690383
356 356 g, t dbSNP:757444137
359 359 c, g dbSNP:752546362
370 370 c, t dbSNP:765086319
371 371 a, g dbSNP:370682047
375 375 a, c dbSNP:138892784
378 378 c, t dbSNP:766313852
391 391 a, g, t dbSNP:565757665
395 395 a, g dbSNP:771625273
404 404 c, t dbSNP:139261750
405 405 a, g dbSNP:774718977
413 413 c, g, t dbSNP:749620420
425 425 c, t dbSNP:372445274
426 426 g, t dbSNP:779856022
429 429 a, g dbSNP:756025962
433 433 g, t dbSNP:541338991
435 435 a, g dbSNP:767410582
438 438 c, t dbSNP:761379296
439 439 a, g dbSNP:751056475
440 440 a, c dbSNP:759577964
453 453 c, g, t dbSNP:762526718
454 454 a, g dbSNP:775750642
459 459 c, g dbSNP:770149919
474 474 a, c dbSNP:369504312
477 477 a, c dbSNP:376180814
478 478 c, t dbSNP:771564922
480 480 c, g, t dbSNP:371803211
485 485 c, g dbSNP:772449065
492 492 c, t dbSNP:748364155
507 507 c, t dbSNP:778973971
508 508 c, t dbSNP:387907039
509 509 a, g dbSNP:572112273
512 512 c, t dbSNP:387907172
513 513 c, g, t dbSNP:781172621
514 514 c, g dbSNP:757119964
523 523 a, g dbSNP:367933620
529 529 a, c dbSNP:763544424
530 530 c, t dbSNP:758016356
537 537 c, t dbSNP:142632840
538 538 a, g dbSNP:201126702
545 545 a, g dbSNP:760000001
546 546 c, t dbSNP:776851677
555 555 c, g dbSNP:146684287
559 559 c, g, t dbSNP:761217557
568 568 a, g dbSNP:773459488
573 573 a, g dbSNP:772321218
576 576 c, t dbSNP:371293493
577 577 a, g dbSNP:774693133
578 578 a, g dbSNP:768757432
587 587 a, g dbSNP:761645282
589 589 a, g dbSNP:780906296
590 590 a, c dbSNP:555874983
596 596 c, t dbSNP:762169178
599 599 g, t dbSNP:149133141
603 603 c, t dbSNP:771402018
604 604 a, g dbSNP:762987810
608 608 c, t dbSNP:142644174
618 618 c, g dbSNP:572400760
619 619 a, t dbSNP:775735494
625 625 a, c, g dbSNP:373152103
629 629 c, t dbSNP:374386471
636 636 c, t dbSNP:541507213
648 648 a, c, t dbSNP:544646155
649 649 a, g, t dbSNP:199815871
651 651 a, c, g, t dbSNP:370973830
652 652 a, g dbSNP:138244546
661 661 c, t dbSNP:781362098
663 663 a, g dbSNP:757812676
667 667 a, g dbSNP:573296085
668 668 g, t dbSNP:764638921
670 670 a, c dbSNP:763075924
672 672 c, g, t dbSNP:142533482
675 675 c, t dbSNP:759692923
679 679 c, g dbSNP:772704885
684 684 c, t dbSNP:2301865
689 689 c, t dbSNP:761649745
691 691 a, g dbSNP:373270982
693 693 a, g dbSNP:11554621
698 698 g, t dbSNP:573835278
703 703 a, g dbSNP:199908318
708 708 c, t dbSNP:748813351
710 710 c, t dbSNP:202048141
711 711 a, g dbSNP:200053706
714 714 g, t dbSNP:745476992
720 720 c, t dbSNP:780864815
726 726 c, t dbSNP:149698079
729 729 c, t dbSNP:780860426
730 730 a, g dbSNP:150427439
732 732 c, g dbSNP:758832450
733 733 c, t dbSNP:752782914
738 738 c, t dbSNP:11554620
747 747 c, t dbSNP:755023635
748 748 c, t dbSNP:200503137
756 756 c, t dbSNP:557612137
770 770 c, t dbSNP:373010581
771 771 a, g dbSNP:766614299
772 772 c, t dbSNP:761580869
773 773 c, t dbSNP:774131656
774 774 a, g dbSNP:144071567
775 775 c, t dbSNP:530329233
779 779 a, c dbSNP:775170242
780 780 a, g dbSNP:769469264
782 782 g, t dbSNP:139459274
795 795 c, g dbSNP:768684986
797 797 a, g dbSNP:745359828
798 798 a, c dbSNP:755566191
811 811 c, g dbSNP:770609938
813 813 c, t dbSNP:747337014
814 814 a, g dbSNP:778049749
816 816 c, t dbSNP:746779418
826 826 c, t dbSNP:758924579
835 835 c, g dbSNP:748697810
837 837 c, g dbSNP:779374650
838 838 a, c dbSNP:755111282
860 860 a, c dbSNP:375479162
865 865 c, t dbSNP:372391824
875 875 a, g dbSNP:756322979
876 876 c, t dbSNP:558457701
883 883 c, t dbSNP:781542407
888 888 c, g, t dbSNP:541753758
892 892 a, c dbSNP:200545714
898 898 c, g dbSNP:150795488
903 903 a, t dbSNP:765267806
917 917 c, t dbSNP:141854925
918 918 c, g dbSNP:140017632
924 924 a, t dbSNP:758814013
930 930 c, g dbSNP:367686182
932 932 c, t dbSNP:376143745
933 933 a, g dbSNP:772910671
936 936 c, t dbSNP:535708941
937 937 a, c, t dbSNP:373844222
938 938 c, t dbSNP:769068248
941 941 c, t dbSNP:749762904
942 942 a, g dbSNP:780394370
953 953 a, c dbSNP:769964325
957 957 c, t dbSNP:745930043
958 958 a, g dbSNP:75856125
962 962 c, t dbSNP:373162068
971 971 c, t dbSNP:757484135
974 974 c, t dbSNP:556409562
975 975 a, g dbSNP:778716914
986 986 a, g dbSNP:528642838
998 998 c, t dbSNP:558364978
1037 1037 g, t dbSNP:762390982
1040 1040 c, t dbSNP:570520893
1074 1074 c, t dbSNP:559457516
1089 1089 a, g dbSNP:527578399
1167 1167 a, g dbSNP:115024147
1204 1204 -, a dbSNP:200454105
1205 1205 acc, ccg dbSNP:386792120
1205 1205 a, c dbSNP:189104271
1206 1206 c, t dbSNP:531435931
1207 1207 c, g dbSNP:80215625
1225 1225 c, t dbSNP:192208552
1226 1226 a, g dbSNP:532087618
1231 1231 a, g dbSNP:115461339
1261 1261 c, t dbSNP:760225366
1290 1290 a, g dbSNP:540276453
1315 1315 c, t dbSNP:563533561
1328 1328 a, t dbSNP:73614641
1371 1371 c, t dbSNP:574506095
1380 1380 g, t dbSNP:764296654
1408 1408 c, t dbSNP:759458453
1430 1430 c, g dbSNP:774188909
1444 1444 a, g dbSNP:187456324
1463 1463 a, c dbSNP:377168724
1464 1464 c, t dbSNP:576582696
1484 1484 c, t dbSNP:556586710
1561 1561 c, g dbSNP:56033857
1563 1563 a, c dbSNP:576971977
1577 1577 a, g dbSNP:553741220
1651 1651 c, g dbSNP:533967309
1663 1663 c, t dbSNP:567990666
1683 1683 c, g dbSNP:772972726
1687 1687 a, g dbSNP:535675896
1702 1702 c, t dbSNP:767455231
1707 1707 a, g dbSNP:150692675
1722 1722 a, c dbSNP:538007529
1727 1727 c, t dbSNP:71392612
1737 1737 c, t dbSNP:568964501
1745 1745 c, g dbSNP:573058036
1754 1754 c, t dbSNP:532244283
1761 1761 c, t dbSNP:141862106
1784 1784 c, g dbSNP:747070392
1791 1791 a, g dbSNP:546605325
1798 1798 a, c dbSNP:780315219
1809 1809 c, g, t dbSNP:529743145
1819 1819 c, t dbSNP:562341602
1842 1842 c, t dbSNP:553167415
1843 1843 a, c dbSNP:182769519
1866 1866 c, g dbSNP:576534015
1882 1882 c, t dbSNP:115575599
1883 1883 a, g dbSNP:546416499
1943 1943 g, t dbSNP:369101225
1982 1982 a, t dbSNP:543103467
1988 1988 c, g dbSNP:1046371
1991 1991 a, g dbSNP:148090175
2024 2024 c, t dbSNP:533902177
2025 2025 a, g dbSNP:747950593
2053 2053 a, g dbSNP:7184172
2055 2055 a, c dbSNP:7189731
2092 2092 c, t dbSNP:537756842
2112 2112 a, g dbSNP:754403053
2117 2117 a, g dbSNP:764667334
2138 2138 a, c dbSNP:1128935
2148 2148 a, g dbSNP:569182380

Target ORF information:

RefSeq Version XM_011523319
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens fatty acid 2-hydroxylase (FA2H), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011523319

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGATGGTG TTCGCCTGTG GAATTCAAGG GGCTCCATGG AGAACGAGCC TGTAGCCCTT 
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC
CACAAGGCAC CCTTCGACGG CTCCCGCCTG GTCTTCCCCC CTGTGCCAGC CTCCCTGGTG
ATCGGCGTCT TCTACTTGTG CATGCAGCTC ATCCTGCCCG AGGCAGTAGG GGGCACTGTG
TTTGCGGGGG GCCTCCTGGG CTACGTCCTC TATGACATGA CCCATTACTA CCTGCACTTT
GGCTCGCCGC ACAAGGGCTC CTACCTGTAC AGCCTGAAGG CCCACCACGT CAAGCACCAC
TTTGCACATC AGAAGTCAGG ATTTGGTATC AGCACTAAAT TGTGGGATTA CTGTTTCCAC
ACCCTCACTC CAGAGAAACC CCACCTGAAG ACGCAGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu26599D
Sequence Information ORF Nucleotide Sequence (Length: 1119bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fatty acid 2-hydroxylase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA890369.1, BC017049.1 and AA857184.1. This sequence is a reference standard in the RefSeqGene project. On Mar 27, 2010 this sequence version replaced gi:205360948. Summary: This gene encodes a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups. Mutations in this gene have been associated with leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia.[provided by RefSeq, Mar 2010]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK058016.1, BC017049.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC
CACAAGGCAC CCTTCGACGG CTCCCGCCTG GTCTTCCCCC CTGTGCCAGC CTCCCTGGTG
ATCGGCGTCT TCTACTTGTG CATGCAGCTC ATCCTGCCCG AGGCAGTAGG GGGCACTGTG
TTTGCGGGGG GCCTCCTGGG CTACGTCCTC TATGACATGA CCCATTACTA CCTGCACTTT
GGCTCGCCGC ACAAGGGCTC CTACCTGTAC AGCCTGAAGG CCCACCACGT CAAGCACCAC
TTTGCACATC AGAAGTCAGG ATTTGGTATC AGCACTAAAT TGTGGGATTA CTGTTTCCAC
ACCCTCACTC CAGAGAAACC CCACCTGAAG ACGCAGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_077282.3
CDS77..1195
Misc Feature(1)11..13(+)
Misc Feature(2)119..331(+)
Misc Feature(3)446..1174(+)
Misc Feature(4)578..640(+)
Misc Feature(5)704..1177(+)
Misc Feature(6)713..775(+)
Misc Feature(7)878..940(+)
Misc Feature(8)944..1006(+)
Exon (1)1..346
Gene:FA2H
Gene Synonym:
Exon (2)347..439
Gene:FA2H
Gene Synonym:
Exon (3)440..582
Gene:FA2H
Gene Synonym:
Exon (4)583..689
Gene:FA2H
Gene Synonym:
Exon (5)690..862
Gene:FA2H
Gene Synonym:
Exon (6)863..1115
Gene:FA2H
Gene Synonym:
Exon (7)1116..2430
Gene:FA2H
Gene Synonym:
Translation
Position Chain Variation Link
8 8 c, t dbSNP:535271682
44 44 c, t dbSNP:569711094
58 58 a, g dbSNP:750069577
82 82 c, t dbSNP:113471134
92 92 c, t dbSNP:773739894
102 102 c, g dbSNP:12930201
105 105 c, t dbSNP:12930196
131 131 c, t dbSNP:763556766
147 147 c, g dbSNP:762615677
158 158 c, t dbSNP:775172785
161 161 c, g dbSNP:550064357
163 163 c, g dbSNP:746288688
179 179 g, t dbSNP:121918217
190 190 c, t dbSNP:777043040
194 194 c, g dbSNP:771357835
198 198 a, g dbSNP:747507656
238 238 a, g dbSNP:778018677
254 254 a, g dbSNP:533048648
260 260 c, t dbSNP:748204186
271 271 a, g dbSNP:779343147
274 274 c, g dbSNP:570454174
278 278 a, g dbSNP:750198250
305 305 c, t dbSNP:929881
308 308 a, g dbSNP:527421775
322 322 a, g dbSNP:751449227
335 335 g, t dbSNP:561760287
340 340 a, g dbSNP:541909354
356 356 a, g dbSNP:761184809
357 357 a, t dbSNP:267604636
359 359 a, g dbSNP:773696633
361 361 c, t dbSNP:772718168
362 362 a, g dbSNP:762343484
365 365 c, g dbSNP:35874850
368 368 a, g dbSNP:768732810
411 411 c, t dbSNP:749305581
413 413 c, t dbSNP:141276237
414 414 a, g dbSNP:147632811
422 422 a, g dbSNP:746868199
423 423 a, t dbSNP:777488679
424 424 c, g dbSNP:758340506
426 426 a, c, t dbSNP:145990810
430 430 c, t dbSNP:778689972
442 442 c, g dbSNP:757737719
443 443 c, t dbSNP:751867997
451 451 a, c dbSNP:764721748
463 463 g, t dbSNP:561163080
475 475 a, g dbSNP:763314788
477 477 g, t dbSNP:199659429
477 477 -, t dbSNP:777658535
480 480 a, g dbSNP:765264675
481 481 a, c, g dbSNP:776875732
482 482 a, c dbSNP:771134202
483 483 a, c dbSNP:761723049
486 486 g, t dbSNP:773958471
487 487 a, g, t dbSNP:749140634
499 499 c, t dbSNP:201250822
500 500 a, g dbSNP:769477853
502 502 c, t dbSNP:142676543
506 506 g, t dbSNP:781059993
509 509 a, g, t dbSNP:751969460
511 511 c, t dbSNP:777935324
513 513 a, c, g dbSNP:201162733
519 519 c, t dbSNP:372350326
520 520 a, g, t dbSNP:138087787
527 527 a, g dbSNP:753898846
532 532 c, t dbSNP:766599125
536 536 c, t dbSNP:387907040
539 539 c, t dbSNP:774231502
547 547 c, g dbSNP:146060468
552 552 a, g, t dbSNP:149561762
555 555 a, t dbSNP:138240770
556 556 c, t dbSNP:775608310
562 562 c, g dbSNP:371859188
563 563 a, g dbSNP:745430083
566 566 c, g, t dbSNP:770624203
573 573 a, c dbSNP:746605230
579 579 c, t dbSNP:778226239
580 580 c, g dbSNP:758742229
586 586 -, ca dbSNP:587776891
605 605 a, g dbSNP:201500782
607 607 a, g dbSNP:775888031
608 608 c, t dbSNP:749744896
613 613 a, g dbSNP:75711361
616 616 g, t dbSNP:150423523
619 619 a, g dbSNP:750511573
625 625 c, t dbSNP:767690383
627 627 g, t dbSNP:757444137
630 630 c, g dbSNP:752546362
641 641 c, t dbSNP:765086319
642 642 a, g dbSNP:370682047
646 646 a, c dbSNP:138892784
649 649 c, t dbSNP:766313852
662 662 a, g, t dbSNP:565757665
666 666 a, g dbSNP:771625273
675 675 c, t dbSNP:139261750
676 676 a, g dbSNP:774718977
684 684 c, g, t dbSNP:749620420
696 696 c, t dbSNP:372445274
697 697 g, t dbSNP:779856022
700 700 a, g dbSNP:756025962
704 704 g, t dbSNP:541338991
706 706 a, g dbSNP:767410582
709 709 c, t dbSNP:761379296
710 710 a, g dbSNP:751056475
711 711 a, c dbSNP:759577964
724 724 c, g, t dbSNP:762526718
725 725 a, g dbSNP:775750642
730 730 c, g dbSNP:770149919
745 745 a, c dbSNP:369504312
748 748 a, c dbSNP:376180814
749 749 c, t dbSNP:771564922
751 751 c, g, t dbSNP:371803211
756 756 c, g dbSNP:772449065
763 763 c, t dbSNP:748364155
778 778 c, t dbSNP:778973971
779 779 c, t dbSNP:387907039
780 780 a, g dbSNP:572112273
783 783 c, t dbSNP:387907172
784 784 c, g, t dbSNP:781172621
785 785 c, g dbSNP:757119964
794 794 a, g dbSNP:367933620
800 800 a, c dbSNP:763544424
801 801 c, t dbSNP:758016356
808 808 c, t dbSNP:142632840
809 809 a, g dbSNP:201126702
816 816 a, g dbSNP:760000001
817 817 c, t dbSNP:776851677
826 826 c, g dbSNP:146684287
830 830 c, g, t dbSNP:761217557
839 839 a, g dbSNP:773459488
844 844 a, g dbSNP:772321218
847 847 c, t dbSNP:371293493
848 848 a, g dbSNP:774693133
849 849 a, g dbSNP:768757432
858 858 a, g dbSNP:761645282
860 860 a, g dbSNP:780906296
861 861 a, c dbSNP:555874983
867 867 c, t dbSNP:762169178
870 870 g, t dbSNP:149133141
874 874 c, t dbSNP:771402018
875 875 a, g dbSNP:762987810
879 879 c, t dbSNP:142644174
889 889 c, g dbSNP:572400760
890 890 a, t dbSNP:775735494
896 896 a, c, g dbSNP:373152103
900 900 c, t dbSNP:374386471
907 907 c, t dbSNP:541507213
919 919 a, c, t dbSNP:544646155
920 920 a, g, t dbSNP:199815871
922 922 a, c, g, t dbSNP:370973830
923 923 a, g dbSNP:138244546
932 932 c, t dbSNP:781362098
934 934 a, g dbSNP:757812676
938 938 a, g dbSNP:573296085
939 939 g, t dbSNP:764638921
941 941 a, c dbSNP:763075924
943 943 c, g, t dbSNP:142533482
946 946 c, t dbSNP:759692923
950 950 c, g dbSNP:772704885
955 955 c, t dbSNP:2301865
960 960 c, t dbSNP:761649745
962 962 a, g dbSNP:373270982
964 964 a, g dbSNP:11554621
969 969 g, t dbSNP:573835278
974 974 a, g dbSNP:199908318
979 979 c, t dbSNP:748813351
981 981 c, t dbSNP:202048141
982 982 a, g dbSNP:200053706
985 985 g, t dbSNP:745476992
991 991 c, t dbSNP:780864815
997 997 c, t dbSNP:149698079
1000 1000 c, t dbSNP:780860426
1001 1001 a, g dbSNP:150427439
1003 1003 c, g dbSNP:758832450
1004 1004 c, t dbSNP:752782914
1009 1009 c, t dbSNP:11554620
1018 1018 c, t dbSNP:755023635
1019 1019 c, t dbSNP:200503137
1027 1027 c, t dbSNP:557612137
1041 1041 c, t dbSNP:373010581
1042 1042 a, g dbSNP:766614299
1043 1043 c, t dbSNP:761580869
1044 1044 c, t dbSNP:774131656
1045 1045 a, g dbSNP:144071567
1046 1046 c, t dbSNP:530329233
1050 1050 a, c dbSNP:775170242
1051 1051 a, g dbSNP:769469264
1053 1053 g, t dbSNP:139459274
1066 1066 c, g dbSNP:768684986
1068 1068 a, g dbSNP:745359828
1069 1069 a, c dbSNP:755566191
1082 1082 c, g dbSNP:770609938
1084 1084 c, t dbSNP:747337014
1085 1085 a, g dbSNP:778049749
1087 1087 c, t dbSNP:746779418
1097 1097 c, t dbSNP:758924579
1106 1106 c, g dbSNP:748697810
1108 1108 c, g dbSNP:779374650
1109 1109 a, c dbSNP:755111282
1131 1131 a, c dbSNP:375479162
1136 1136 c, t dbSNP:372391824
1146 1146 a, g dbSNP:756322979
1147 1147 c, t dbSNP:558457701
1154 1154 c, t dbSNP:781542407
1159 1159 c, g, t dbSNP:541753758
1163 1163 a, c dbSNP:200545714
1169 1169 c, g dbSNP:150795488
1174 1174 a, t dbSNP:765267806
1188 1188 c, t dbSNP:141854925
1189 1189 c, g dbSNP:140017632
1195 1195 a, t dbSNP:758814013
1201 1201 c, g dbSNP:367686182
1203 1203 c, t dbSNP:376143745
1204 1204 a, g dbSNP:772910671
1207 1207 c, t dbSNP:535708941
1208 1208 a, c, t dbSNP:373844222
1209 1209 c, t dbSNP:769068248
1212 1212 c, t dbSNP:749762904
1213 1213 a, g dbSNP:780394370
1224 1224 a, c dbSNP:769964325
1228 1228 c, t dbSNP:745930043
1229 1229 a, g dbSNP:75856125
1233 1233 c, t dbSNP:373162068
1242 1242 c, t dbSNP:757484135
1245 1245 c, t dbSNP:556409562
1246 1246 a, g dbSNP:778716914
1257 1257 a, g dbSNP:528642838
1269 1269 c, t dbSNP:558364978
1308 1308 g, t dbSNP:762390982
1311 1311 c, t dbSNP:570520893
1345 1345 c, t dbSNP:559457516
1360 1360 a, g dbSNP:527578399
1438 1438 a, g dbSNP:115024147
1475 1475 -, a dbSNP:200454105
1476 1476 acc, ccg dbSNP:386792120
1476 1476 a, c dbSNP:189104271
1477 1477 c, t dbSNP:531435931
1478 1478 c, g dbSNP:80215625
1496 1496 c, t dbSNP:192208552
1497 1497 a, g dbSNP:532087618
1502 1502 a, g dbSNP:115461339
1532 1532 c, t dbSNP:760225366
1561 1561 a, g dbSNP:540276453
1586 1586 c, t dbSNP:563533561
1599 1599 a, t dbSNP:73614641
1642 1642 c, t dbSNP:574506095
1651 1651 g, t dbSNP:764296654
1679 1679 c, t dbSNP:759458453
1701 1701 c, g dbSNP:774188909
1715 1715 a, g dbSNP:187456324
1734 1734 a, c dbSNP:377168724
1735 1735 c, t dbSNP:576582696
1755 1755 c, t dbSNP:556586710
1832 1832 c, g dbSNP:56033857
1834 1834 a, c dbSNP:576971977
1848 1848 a, g dbSNP:553741220
1922 1922 c, g dbSNP:533967309
1934 1934 c, t dbSNP:567990666
1954 1954 c, g dbSNP:772972726
1958 1958 a, g dbSNP:535675896
1973 1973 c, t dbSNP:767455231
1978 1978 a, g dbSNP:150692675
1993 1993 a, c dbSNP:538007529
1998 1998 c, t dbSNP:71392612
2008 2008 c, t dbSNP:568964501
2016 2016 c, g dbSNP:573058036
2025 2025 c, t dbSNP:532244283
2032 2032 c, t dbSNP:141862106
2055 2055 c, g dbSNP:747070392
2062 2062 a, g dbSNP:546605325
2069 2069 a, c dbSNP:780315219
2080 2080 c, g, t dbSNP:529743145
2090 2090 c, t dbSNP:562341602
2113 2113 c, t dbSNP:553167415
2114 2114 a, c dbSNP:182769519
2137 2137 c, g dbSNP:576534015
2153 2153 c, t dbSNP:115575599
2154 2154 a, g dbSNP:546416499
2214 2214 g, t dbSNP:369101225
2253 2253 a, t dbSNP:543103467
2259 2259 c, g dbSNP:1046371
2262 2262 a, g dbSNP:148090175
2295 2295 c, t dbSNP:533902177
2296 2296 a, g dbSNP:747950593
2324 2324 a, g dbSNP:7184172
2326 2326 a, c dbSNP:7189731
2363 2363 c, t dbSNP:537756842
2383 2383 a, g dbSNP:754403053
2388 2388 a, g dbSNP:764667334
2409 2409 a, c dbSNP:1128935
2419 2419 a, g dbSNP:569182380

Target ORF information:

RefSeq Version NM_024306
Organism Homo sapiens (human)
Definition Homo sapiens fatty acid 2-hydroxylase (FA2H), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_024306

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCCCCCG CTCCGCCCCC CGCCGCCTCC TTCTCGCCCT CCGAGGTCCA GCGGCGCCTG 
GCGGCCGGCG CGTGCTGGGT CCGCCGCGGG GCCCGCCTCT ACGACCTCTC CAGCTTCGTG
CGGCACCACC CGGGGGGCGA GCAGCTGCTG CGGGCCAGGG CGGGCCAGGA CATCAGCGCC
GACCTGGACG GGCCGCCGCA CAGGCACTCG GCCAACGCGC GCCGCTGGCT GGAGCAGTAC
TACGTGGGAG AGCTCCGCGG GGAGCAGCAG GGCTCCATGG AGAACGAGCC TGTAGCCCTT
GAGGAAACTC AGAAGACAGA TCCTGCTATG GAACCACGGT TCAAAGTGGT GGATTGGGAC
AAGGACCTGG TGGACTGGCG AAAGCCTCTC CTGTGGCAGG TGGGCCACTT GGGAGAGAAG
TACGATGAGT GGGTTCACCA GCCGGTGACC AGGCCCATCC GCCTCTTCCA CTCAGACCTC
ATTGAGGGCC TCTCTAAGAC TGTCTGGTAC AGTGTCCCCA TCATCTGGGT GCCCCTGGTG
CTGTATCTCA GCTGGTCCTA CTACCGAACC TTTGCCCAGG GCAACGTCCG ACTCTTCACG
TCATTTACAA CAGAGTACAC GGTGGCAGTG CCCAAGTCCA TGTTCCCCGG GCTCTTCATG
CTGGGGACAT TCCTCTGGAG CCTCATCGAG TACCTCATCC ACCGCTTCCT GTTCCACATG
AAGCCCCCCA GCGACAGCTA TTACCTCATC ATGCTGCACT TCGTCATGCA CGGCCAGCAC
CACAAGGCAC CCTTCGACGG CTCCCGCCTG GTCTTCCCCC CTGTGCCAGC CTCCCTGGTG
ATCGGCGTCT TCTACTTGTG CATGCAGCTC ATCCTGCCCG AGGCAGTAGG GGGCACTGTG
TTTGCGGGGG GCCTCCTGGG CTACGTCCTC TATGACATGA CCCATTACTA CCTGCACTTT
GGCTCGCCGC ACAAGGGCTC CTACCTGTAC AGCCTGAAGG CCCACCACGT CAAGCACCAC
TTTGCACATC AGAAGTCAGG ATTTGGTATC AGCACTAAAT TGTGGGATTA CTGTTTCCAC
ACCCTCACTC CAGAGAAACC CCACCTGAAG ACGCAGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.