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RNASEH2B cDNA ORF clone, Homo sapiens (human)

Gene Symbol RNASEH2B
Entrez Gene ID 79621
Full Name ribonuclease H2, subunit B
Synonyms AGS2, DLEU8
General protein information
Preferred Names
ribonuclease H2 subunit B
Names
ribonuclease H2 subunit B
RNase H2 subunit B
ribonuclease HI subunit B
deleted in lymphocytic leukemia 8
Aicardi-Goutieres syndrome 2 protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

13

13q14.3

Summary RNase H2 is composed of a single catalytic subunit (A) and two non-catalytic subunits (B and C) and specifically degrades the RNA of RNA:DNA hybrids. The protein encoded by this gene is the non-catalytic B subunit of RNase H2, which is thought to play a role in DNA replication. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Aicardi-Goutieres syndrome type 2 (AGS2). [provided by RefSeq, Nov 2008]. lac of sum
Disorder MIM:

610326

Disorder Html: Aicardi-Goutieres syndrome 2, 610181 (3)





The following RNASEH2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNASEH2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu47264 XM_006719867 PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu47265 XM_005266524 PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu47266 XM_005266525 PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu71940 XM_011535229 PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu71941 XM_011535230 PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu71942 XM_011535231 PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu71943 XM_011535232 PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu71944 XM_011535233 PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99.00
OHu71945 XM_011535234 PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99.00
OHu23058 NM_001142279 Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269.00
OHu23224 NM_024570 Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $309.00

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**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu47264
Accession Version XM_006719867.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 921bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ribonuclease H2 subunit B isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578825006. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACACAGT TAAAGGAATT TGGCCTAGGG AGTGCAAGCC AGAAAGAATA TTTAAAAGAT 
GCTTCAAAGA AGATGAAAAA TGGGCTAATG TTTGTAAAAC TGGTTAACCC CTGTTCAGGA
GAAGGAGCCA TTTACTTGTT CAATATGTGT CTACAGCAGC TGTTTGAAGT AAAAGTTTTC
AAGGAAAAAC ACCATTCTTG GTTTATAAAT CAATCAGTTC AATCAGGAGG TCTTCTCCAT
TTTGCCACAC CTGTGGATCC TCTATTTCTG CTTCTCCACT ACCTCATAAA GGCTGATAAG
GAGGGGAAGT TTCAGCCCCT TGATCAAGTT GTGGTGGATA ACGTGTTTCC AAATTGCATC
TTGTTGCTGA AACTTCCTGG ACTTGAGAAG TTACTTCATC ATGTGACAGA GGAAAAAGGT
AATCCAGAAA TAGACAACAA GAAATATTAC AAGTACAGCA AAGAGAAGAC ATTAAAGTGG
CTGGAAAAAA AGGTTAATCA AACTGTGGCA GCATTAAAAA CCAATAATGT GAATGTCAGT
TCCCGGGTAC AGTCAACTGC ATTTTTCTCT GGTGACCAAG CTTCCACTGA CAAGGAAGAG
GATTATATTC GTTATGCCCA TGGTCTGATA TCTGACTACA TCCCTAAAGA ATTAAGTGAT
GACTTATCTA AATACTTAAA GCTTCCAGAA CCTTCAGCCT CATTGCCAAA TCCTCCATCA
AAGAAAATAA AGTTATCAGA TGAGCCTGTA GAAGCAAAAG AAGATTACAC TAAGTTTAAT
ACTAAAGATT TGAAGACTGA AAAGAAAAAT AGCAAAATGA CTGCAGCTCA GAAGGCTTTG
GCTAAAGTTG ACAAGAGTGG AATGAAAAGT ATTGATACCT TTTTTGGGGT AAAAAATAAA
AAAAAAATTG GAAAGGTTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_006719930.1
CDS16..936
Misc Feature(1)100..903(+)
Misc Feature(2)112..222(+)
Translation
Position Chain Variation Link
23 23 a, g dbSNP:527919022
49 49 a, g dbSNP:180678050
53 53 a, g dbSNP:185253165
66 66 a, t dbSNP:778329909
69 69 a, g dbSNP:748052389
76 76 a, g dbSNP:763308917
80 80 c, g dbSNP:771472509
82 82 -, aag dbSNP:776706264
86 86 a, c dbSNP:772952262
89 89 c, t dbSNP:542600186
92 92 a, g dbSNP:377417708
93 93 a, g dbSNP:775880092
95 95 a, c dbSNP:763229293
99 99 a, g dbSNP:764270783
103 103 a, g dbSNP:774427890
124 124 c, t dbSNP:762281569
125 125 a, c dbSNP:79564863
126 126 a, c dbSNP:199839728
129 129 a, t dbSNP:74876702
131 131 c, t dbSNP:575985863
133 133 -, g dbSNP:762857414
150 150 c, t dbSNP:772503413
153 153 a, g dbSNP:35416748
158 158 a, g dbSNP:770080674
161 161 c, t dbSNP:374465686
167 167 c, t dbSNP:537203779
169 169 c, t dbSNP:75326546
170 170 a, g dbSNP:759251667
174 174 a, g dbSNP:141881672
176 176 g, t dbSNP:75325951
177 177 c, g dbSNP:556877869
180 180 c, t dbSNP:368589166
186 186 a, g dbSNP:35595258
190 190 a, g dbSNP:541903338
190 190 -, g dbSNP:758911416
199 199 c, g dbSNP:145465454
200 200 a, g dbSNP:148811739
215 215 g, t dbSNP:78071087
231 231 a, g dbSNP:757200430
237 237 a, g dbSNP:781187392
239 239 -, aggt dbSNP:780704541
244 244 a, g dbSNP:76158094
247 247 c, g dbSNP:143523027
248 248 g, t dbSNP:747061909
254 254 a, g dbSNP:77931005
260 260 c, g dbSNP:777149786
264 264 -, c dbSNP:781518432
265 265 c, t dbSNP:746338829
266 266 c, g, t dbSNP:770203126
270 270 g, t dbSNP:374098961
274 274 c, g dbSNP:763646959
285 285 c, g dbSNP:774041308
301 301 a, g dbSNP:761380890
305 305 a, g dbSNP:767086382
311 311 a, c dbSNP:201078944
314 314 a, c dbSNP:755936337
321 321 a, g dbSNP:760734846
345 345 c, t dbSNP:766196712
349 349 c, g dbSNP:753716315
353 353 a, g dbSNP:786205483
357 357 c, t dbSNP:759273327
358 358 a, g dbSNP:764980431
362 362 g, t dbSNP:751788888
367 367 a, g dbSNP:757415855
372 372 c, t dbSNP:781310346
373 373 a, g dbSNP:569343532
384 384 a, g dbSNP:756682554
391 391 c, g dbSNP:780398368
392 392 c, g dbSNP:749750512
400 400 a, g dbSNP:769173136
404 404 a, c dbSNP:199935006
409 409 c, t dbSNP:78705382
425 425 a, t dbSNP:200535256
426 426 c, g dbSNP:754434417
437 437 a, c dbSNP:746633003
442 442 c, g dbSNP:770355902
452 452 a, g dbSNP:146451037
453 453 c, t dbSNP:759529625
455 455 a, g dbSNP:769721789
462 462 c, t dbSNP:202232001
465 465 c, t dbSNP:775300215
473 473 g, t dbSNP:76219783
480 480 a, g dbSNP:762711560
482 482 a, c dbSNP:75971463
485 485 c, t dbSNP:79310911
495 495 g, t dbSNP:763896138
500 500 a, g dbSNP:78315883
503 503 a, c dbSNP:760884054
512 512 a, g dbSNP:775424614
519 519 c, t dbSNP:200390483
520 520 a, g dbSNP:141049110
525 525 a, g dbSNP:768340427
526 526 a, g dbSNP:75184679
544 544 a, g dbSNP:77377571
546 546 a, g dbSNP:760862553
549 549 c, t dbSNP:766618424
551 551 g, t dbSNP:74555752
552 552 c, g dbSNP:566790226
553 553 a, g dbSNP:759712178
559 559 c, t dbSNP:765368182
560 560 a, g dbSNP:138649673
561 561 c, g dbSNP:141367253
564 564 -, acagtca dbSNP:765643940
564 564 a, g dbSNP:764428875
568 568 a, t dbSNP:555463211
569 569 c, g dbSNP:756903057
573 573 g, t dbSNP:780872918
586 586 g, t dbSNP:745471919
609 609 a, g dbSNP:755648468
612 612 a, g dbSNP:779468408
613 613 a, g dbSNP:17857128
614 614 a, c dbSNP:371740577
615 615 g, t dbSNP:762258182
616 616 a, g dbSNP:376240726
619 619 g, t dbSNP:750077795
625 625 c, t dbSNP:769632378
626 626 a, g dbSNP:755705496
629 629 a, t dbSNP:779596970
633 633 c, t dbSNP:753339416
635 635 a, g dbSNP:200648780
642 642 a, g dbSNP:376122649
646 646 g, t dbSNP:778933609
652 652 c, t dbSNP:77391331
656 656 a, c, t dbSNP:747995413
658 658 c, g dbSNP:777655486
661 661 a, g dbSNP:746153565
674 674 a, g dbSNP:770138442
676 676 a, g dbSNP:775757158
680 680 -, a dbSNP:748406884
680 680 a, t dbSNP:763070137
682 682 c, t dbSNP:768565639
694 694 a, c dbSNP:774950202
696 696 a, g dbSNP:768783637
702 702 a, g dbSNP:774234566
704 704 a, g dbSNP:748746326
716 716 c, g, t dbSNP:372632599
717 717 a, g dbSNP:761005167
731 731 c, t dbSNP:190327832
741 741 a, c dbSNP:748144224
753 753 a, g dbSNP:200721645
759 759 a, g dbSNP:146378238
760 760 c, g dbSNP:747450548
763 763 g, t dbSNP:771299667
783 783 c, t dbSNP:776847998
784 784 a, g dbSNP:150363383
785 785 c, g dbSNP:183734174
805 805 c, g, t dbSNP:775119865
815 815 a, g dbSNP:763654606
821 821 a, g dbSNP:761906095
822 822 a, g dbSNP:559000645
827 827 a, g dbSNP:200802557
831 831 a, g dbSNP:750279427
840 840 a, c dbSNP:760409807
845 845 a, g dbSNP:766157957
850 850 a, g dbSNP:752930177
851 851 c, t dbSNP:758605855
856 856 g, t dbSNP:144408326
865 865 a, g dbSNP:201190805
885 885 c, t dbSNP:757835049
886 886 a, g dbSNP:781574666
892 892 a, g dbSNP:370507842
896 896 a, t dbSNP:770055331
899 899 c, t dbSNP:780504013
901 901 a, g dbSNP:749003546
904 904 -, t dbSNP:768221761
911 911 a, c dbSNP:768415436
912 912 -, a, aa dbSNP:200320729
912 912 a, t dbSNP:112937854
913 913 -, a dbSNP:761386077
913 913 a, t dbSNP:774043353
922 922 -, a dbSNP:75254367
922 922 a, g dbSNP:761204571
925 925 -, ttat dbSNP:772980492
925 925 a, g dbSNP:771560732
948 948 c, t dbSNP:773052815
969 969 g, t dbSNP:529254634
976 976 a, t dbSNP:766145608
985 985 a, g dbSNP:753627853
989 989 c, t dbSNP:549023975
990 990 g, t dbSNP:569240707
999 999 c, g, t dbSNP:751735556
1003 1003 a, g dbSNP:781270130
1012 1012 a, t dbSNP:750903767
1022 1022 g, t dbSNP:756627118
1038 1038 -, c dbSNP:763452841
1046 1046 a, g dbSNP:761886682
1082 1082 c, t dbSNP:780358371
1182 1182 a, g dbSNP:147825612
1187 1187 g, t dbSNP:747397214
1209 1209 c, t dbSNP:551658315

Target ORF information:

RefSeq Version XM_006719867
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006719867

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACACAGT TAAAGGAATT TGGCCTAGGG AGTGCAAGCC AGAAAGAATA TTTAAAAGAT 
GCTTCAAAGA AGATGAAAAA TGGGCTAATG TTTGTAAAAC TGGTTAACCC CTGTTCAGGA
GAAGGAGCCA TTTACTTGTT CAATATGTGT CTACAGCAGC TGTTTGAAGT AAAAGTTTTC
AAGGAAAAAC ACCATTCTTG GTTTATAAAT CAATCAGTTC AATCAGGAGG TCTTCTCCAT
TTTGCCACAC CTGTGGATCC TCTATTTCTG CTTCTCCACT ACCTCATAAA GGCTGATAAG
GAGGGGAAGT TTCAGCCCCT TGATCAAGTT GTGGTGGATA ACGTGTTTCC AAATTGCATC
TTGTTGCTGA AACTTCCTGG ACTTGAGAAG TTACTTCATC ATGTGACAGA GGAAAAAGGT
AATCCAGAAA TAGACAACAA GAAATATTAC AAGTACAGCA AAGAGAAGAC ATTAAAGTGG
CTGGAAAAAA AGGTTAATCA AACTGTGGCA GCATTAAAAA CCAATAATGT GAATGTCAGT
TCCCGGGTAC AGTCAACTGC ATTTTTCTCT GGTGACCAAG CTTCCACTGA CAAGGAAGAG
GATTATATTC GTTATGCCCA TGGTCTGATA TCTGACTACA TCCCTAAAGA ATTAAGTGAT
GACTTATCTA AATACTTAAA GCTTCCAGAA CCTTCAGCCT CATTGCCAAA TCCTCCATCA
AAGAAAATAA AGTTATCAGA TGAGCCTGTA GAAGCAAAAG AAGATTACAC TAAGTTTAAT
ACTAAAGATT TGAAGACTGA AAAGAAAAAT AGCAAAATGA CTGCAGCTCA GAAGGCTTTG
GCTAAAGTTG ACAAGAGTGG AATGAAAAGT ATTGATACCT TTTTTGGGGT AAAAAATAAA
AAAAAAATTG GAAAGGTTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47265
Accession Version XM_005266524.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 882bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ribonuclease H2 subunit B isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530402643. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAG GTTAATCAAA CTGTGGCAGC ATTAAAAACC
AATAATGTGA ATGTCAGTTC CCGGGTACAG TCAACTGCAT TTTTCTCTGG TGACCAAGCT
TCCACTGACA AGGAAGAGGA TTATATTCGT TATGCCCATG GTCTGATATC TGACTACATC
CCTAAAGAAT TAAGTGATGA CTTATCTAAA TACTTAAAGC TTCCAGAACC TTCAGCCTCA
TTGCCAAATC CTCCATCAAA GAAAATAAAG TTATCAGATG AGCCTGTAGA AGCAAAAGAA
GATTACACTA AGTTTAATAC TAAAGATTTG AAGACTGAAA AGCTAGGTCT ACCAACTGGT
CATTTTGTGA TGGCAGCACA AAGACAGAAA AGGGGCAAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005266581.1
CDS322..1203
Misc Feature(1)370..>882(+)
Misc Feature(2)376..546(+)
Translation
Position Chain Variation Link
30 30 c, g dbSNP:544448663
48 48 c, g dbSNP:540984248
94 94 a, c dbSNP:577725827
95 95 a, g dbSNP:564346046
112 112 c, g dbSNP:112702177
114 114 a, g dbSNP:11544097
205 205 c, g dbSNP:553446261
210 210 c, t dbSNP:184573722
246 246 c, g dbSNP:529317356
257 257 c, t dbSNP:549441721
297 297 c, t dbSNP:562869389
313 313 a, g dbSNP:765552524
328 328 a, g dbSNP:531904107
341 341 a, g dbSNP:551573692
360 360 c, t dbSNP:571566229
367 367 c, t dbSNP:763103642
372 372 a, g dbSNP:764223334
373 373 a, t dbSNP:758877268
390 390 a, t dbSNP:778329909
393 393 a, g dbSNP:748052389
400 400 a, g dbSNP:763308917
404 404 c, g dbSNP:771472509
406 406 -, aag dbSNP:776706264
410 410 a, c dbSNP:772952262
413 413 c, t dbSNP:542600186
416 416 a, g dbSNP:377417708
417 417 a, g dbSNP:775880092
419 419 a, c dbSNP:763229293
423 423 a, g dbSNP:764270783
427 427 a, g dbSNP:774427890
448 448 c, t dbSNP:762281569
449 449 a, c dbSNP:79564863
450 450 a, c dbSNP:199839728
453 453 a, t dbSNP:74876702
455 455 c, t dbSNP:575985863
457 457 -, g dbSNP:762857414
474 474 c, t dbSNP:772503413
477 477 a, g dbSNP:35416748
482 482 a, g dbSNP:770080674
485 485 c, t dbSNP:374465686
491 491 c, t dbSNP:537203779
493 493 c, t dbSNP:75326546
494 494 a, g dbSNP:759251667
498 498 a, g dbSNP:141881672
500 500 g, t dbSNP:75325951
501 501 c, g dbSNP:556877869
504 504 c, t dbSNP:368589166
510 510 a, g dbSNP:35595258
514 514 a, g dbSNP:541903338
514 514 -, g dbSNP:758911416
523 523 c, g dbSNP:145465454
524 524 a, g dbSNP:148811739
539 539 g, t dbSNP:78071087
555 555 a, g dbSNP:757200430
561 561 a, g dbSNP:781187392
563 563 -, aggt dbSNP:780704541
568 568 a, g dbSNP:76158094
571 571 c, g dbSNP:143523027
572 572 g, t dbSNP:747061909
578 578 a, g dbSNP:77931005
584 584 c, g dbSNP:777149786
588 588 -, c dbSNP:781518432
589 589 c, t dbSNP:746338829
590 590 c, g, t dbSNP:770203126
594 594 g, t dbSNP:374098961
598 598 c, g dbSNP:763646959
609 609 c, g dbSNP:774041308
625 625 a, g dbSNP:761380890
629 629 a, g dbSNP:767086382
635 635 a, c dbSNP:201078944
638 638 a, c dbSNP:755936337
645 645 a, g dbSNP:760734846
669 669 c, t dbSNP:766196712
673 673 c, g dbSNP:753716315
677 677 a, g dbSNP:786205483
681 681 c, t dbSNP:759273327
682 682 a, g dbSNP:764980431
686 686 g, t dbSNP:751788888
691 691 a, g dbSNP:757415855
696 696 c, t dbSNP:781310346
697 697 a, g dbSNP:569343532
708 708 a, g dbSNP:756682554
715 715 c, g dbSNP:780398368
716 716 c, g dbSNP:749750512
724 724 a, g dbSNP:769173136
728 728 a, c dbSNP:199935006
733 733 c, t dbSNP:78705382
749 749 a, t dbSNP:200535256
750 750 c, g dbSNP:754434417
761 761 a, c dbSNP:746633003
766 766 c, g dbSNP:770355902
776 776 a, g dbSNP:146451037
777 777 c, t dbSNP:759529625
779 779 a, g dbSNP:769721789
786 786 c, t dbSNP:202232001
789 789 c, t dbSNP:775300215
797 797 g, t dbSNP:76219783
804 804 a, g dbSNP:762711560
806 806 a, c dbSNP:75971463
809 809 c, t dbSNP:79310911
819 819 g, t dbSNP:763896138
824 824 a, g dbSNP:78315883
827 827 a, c dbSNP:760884054
836 836 a, g dbSNP:775424614
843 843 c, t dbSNP:200390483
844 844 a, g dbSNP:141049110
849 849 a, g dbSNP:768340427
850 850 a, g dbSNP:75184679
868 868 a, g dbSNP:77377571
870 870 a, g dbSNP:760862553
873 873 c, t dbSNP:766618424
875 875 g, t dbSNP:74555752
876 876 c, g dbSNP:566790226
877 877 a, g dbSNP:759712178
883 883 c, t dbSNP:765368182
884 884 a, g dbSNP:138649673
885 885 c, g dbSNP:141367253
888 888 -, acagtca dbSNP:765643940
888 888 a, g dbSNP:764428875
892 892 a, t dbSNP:555463211
893 893 c, g dbSNP:756903057
897 897 g, t dbSNP:780872918
910 910 g, t dbSNP:745471919
933 933 a, g dbSNP:755648468
936 936 a, g dbSNP:779468408
937 937 a, g dbSNP:17857128
938 938 a, c dbSNP:371740577
939 939 g, t dbSNP:762258182
940 940 a, g dbSNP:376240726
943 943 g, t dbSNP:750077795
949 949 c, t dbSNP:769632378
950 950 a, g dbSNP:755705496
953 953 a, t dbSNP:779596970
957 957 c, t dbSNP:753339416
959 959 a, g dbSNP:200648780
966 966 a, g dbSNP:376122649
970 970 g, t dbSNP:778933609
976 976 c, t dbSNP:77391331
980 980 a, c, t dbSNP:747995413
982 982 c, g dbSNP:777655486
985 985 a, g dbSNP:746153565
998 998 a, g dbSNP:770138442
1000 1000 a, g dbSNP:775757158
1004 1004 -, a dbSNP:748406884
1004 1004 a, t dbSNP:763070137
1006 1006 c, t dbSNP:768565639
1018 1018 a, c dbSNP:774950202
1020 1020 a, g dbSNP:768783637
1026 1026 a, g dbSNP:774234566
1028 1028 a, g dbSNP:748746326
1040 1040 c, g, t dbSNP:372632599
1041 1041 a, g dbSNP:761005167
1055 1055 c, t dbSNP:190327832
1065 1065 a, c dbSNP:748144224
1077 1077 a, g dbSNP:200721645
1083 1083 a, g dbSNP:146378238
1084 1084 c, g dbSNP:747450548
1087 1087 g, t dbSNP:771299667
1107 1107 c, t dbSNP:776847998
1108 1108 a, g dbSNP:150363383
1109 1109 c, g dbSNP:183734174
1129 1129 c, g, t dbSNP:775119865
1139 1139 a, g dbSNP:763654606
1199 1199 a, c dbSNP:778729628
1202 1202 a, g dbSNP:377006588
1204 1204 -, tgg dbSNP:766811359
1208 1208 a, g dbSNP:370009090
1219 1219 a, g dbSNP:559536664
1222 1222 a, c dbSNP:141473699
1230 1230 -, g dbSNP:374825738
1316 1316 g, t dbSNP:542131533
1317 1317 g, t dbSNP:375966200
1329 1329 a, g dbSNP:757499643
1354 1354 g, t dbSNP:530824994
1383 1383 c, t dbSNP:550602087
1384 1384 a, g dbSNP:779197062
1407 1407 c, g dbSNP:555426386
1413 1413 c, t dbSNP:192376633
1445 1445 c, t dbSNP:184044774
1469 1469 c, t dbSNP:781732921
1487 1487 c, t dbSNP:533284692
1501 1501 a, g dbSNP:548955070
1507 1507 a, c dbSNP:188678952
1512 1512 a, g dbSNP:150233508
1519 1519 a, g dbSNP:115601793
1532 1532 a, g dbSNP:776130529
1536 1536 c, t dbSNP:377552193
1540 1540 a, t dbSNP:537038741
1576 1576 -, ctta dbSNP:574696297
1596 1596 c, t dbSNP:370311386
1608 1608 a, g dbSNP:746488612
1610 1610 a, g dbSNP:557026437
1611 1611 c, t dbSNP:576999314
1633 1633 a, g dbSNP:768156129
1645 1645 a, g dbSNP:374087970
1648 1648 c, t dbSNP:181458623
1649 1649 a, g dbSNP:572999385
1667 1667 c, t dbSNP:370687538
1671 1671 a, g dbSNP:149475110
1690 1690 c, t dbSNP:575192646

Target ORF information:

RefSeq Version XM_005266524
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005266524

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAG GTTAATCAAA CTGTGGCAGC ATTAAAAACC
AATAATGTGA ATGTCAGTTC CCGGGTACAG TCAACTGCAT TTTTCTCTGG TGACCAAGCT
TCCACTGACA AGGAAGAGGA TTATATTCGT TATGCCCATG GTCTGATATC TGACTACATC
CCTAAAGAAT TAAGTGATGA CTTATCTAAA TACTTAAAGC TTCCAGAACC TTCAGCCTCA
TTGCCAAATC CTCCATCAAA GAAAATAAAG TTATCAGATG AGCCTGTAGA AGCAAAAGAA
GATTACACTA AGTTTAATAC TAAAGATTTG AAGACTGAAA AGCTAGGTCT ACCAACTGGT
CATTTTGTGA TGGCAGCACA AAGACAGAAA AGGGGCAAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47266
Accession Version XM_005266525.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ribonuclease H2 subunit B isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530402645. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAG GTTAATCAAA CTGTGGCAGC ATTAAAAACC
AATAATGTGA ATGTCAGTTC CCGGGTACAG TCAACTGCAT TTTTCTCTGG TGACCAAGCT
TCCACTGACA AGGAAGAGGA TTATATTCGT TATGCCCATG GTCTGATATC TGACTACATC
CCTAAAGAAT TAAGTGATGA CTTATCTAAA TACTTAAAGC TTCCAGAACC TTCAGCCTCA
TTGCCAAATC CTCCATCAAA GAAAATAAAG TTATCAGATG AGCCTGTAGA AGCAAAAGAA
GATTACACTA AGTTTAATAC TAAAGATTTG AAGACTGAAA AGATGGCAGC ACAAAGACAG
AAAAGGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005266582.1
CDS322..1176
Misc Feature(1)370..>882(+)
Misc Feature(2)376..546(+)
Translation
Position Chain Variation Link
30 30 c, g dbSNP:544448663
48 48 c, g dbSNP:540984248
94 94 a, c dbSNP:577725827
95 95 a, g dbSNP:564346046
112 112 c, g dbSNP:112702177
114 114 a, g dbSNP:11544097
205 205 c, g dbSNP:553446261
210 210 c, t dbSNP:184573722
246 246 c, g dbSNP:529317356
257 257 c, t dbSNP:549441721
297 297 c, t dbSNP:562869389
313 313 a, g dbSNP:765552524
328 328 a, g dbSNP:531904107
341 341 a, g dbSNP:551573692
360 360 c, t dbSNP:571566229
367 367 c, t dbSNP:763103642
372 372 a, g dbSNP:764223334
373 373 a, t dbSNP:758877268
390 390 a, t dbSNP:778329909
393 393 a, g dbSNP:748052389
400 400 a, g dbSNP:763308917
404 404 c, g dbSNP:771472509
406 406 -, aag dbSNP:776706264
410 410 a, c dbSNP:772952262
413 413 c, t dbSNP:542600186
416 416 a, g dbSNP:377417708
417 417 a, g dbSNP:775880092
419 419 a, c dbSNP:763229293
423 423 a, g dbSNP:764270783
427 427 a, g dbSNP:774427890
448 448 c, t dbSNP:762281569
449 449 a, c dbSNP:79564863
450 450 a, c dbSNP:199839728
453 453 a, t dbSNP:74876702
455 455 c, t dbSNP:575985863
457 457 -, g dbSNP:762857414
474 474 c, t dbSNP:772503413
477 477 a, g dbSNP:35416748
482 482 a, g dbSNP:770080674
485 485 c, t dbSNP:374465686
491 491 c, t dbSNP:537203779
493 493 c, t dbSNP:75326546
494 494 a, g dbSNP:759251667
498 498 a, g dbSNP:141881672
500 500 g, t dbSNP:75325951
501 501 c, g dbSNP:556877869
504 504 c, t dbSNP:368589166
510 510 a, g dbSNP:35595258
514 514 a, g dbSNP:541903338
514 514 -, g dbSNP:758911416
523 523 c, g dbSNP:145465454
524 524 a, g dbSNP:148811739
539 539 g, t dbSNP:78071087
555 555 a, g dbSNP:757200430
561 561 a, g dbSNP:781187392
563 563 -, aggt dbSNP:780704541
568 568 a, g dbSNP:76158094
571 571 c, g dbSNP:143523027
572 572 g, t dbSNP:747061909
578 578 a, g dbSNP:77931005
584 584 c, g dbSNP:777149786
588 588 -, c dbSNP:781518432
589 589 c, t dbSNP:746338829
590 590 c, g, t dbSNP:770203126
594 594 g, t dbSNP:374098961
598 598 c, g dbSNP:763646959
609 609 c, g dbSNP:774041308
625 625 a, g dbSNP:761380890
629 629 a, g dbSNP:767086382
635 635 a, c dbSNP:201078944
638 638 a, c dbSNP:755936337
645 645 a, g dbSNP:760734846
669 669 c, t dbSNP:766196712
673 673 c, g dbSNP:753716315
677 677 a, g dbSNP:786205483
681 681 c, t dbSNP:759273327
682 682 a, g dbSNP:764980431
686 686 g, t dbSNP:751788888
691 691 a, g dbSNP:757415855
696 696 c, t dbSNP:781310346
697 697 a, g dbSNP:569343532
708 708 a, g dbSNP:756682554
715 715 c, g dbSNP:780398368
716 716 c, g dbSNP:749750512
724 724 a, g dbSNP:769173136
728 728 a, c dbSNP:199935006
733 733 c, t dbSNP:78705382
749 749 a, t dbSNP:200535256
750 750 c, g dbSNP:754434417
761 761 a, c dbSNP:746633003
766 766 c, g dbSNP:770355902
776 776 a, g dbSNP:146451037
777 777 c, t dbSNP:759529625
779 779 a, g dbSNP:769721789
786 786 c, t dbSNP:202232001
789 789 c, t dbSNP:775300215
797 797 g, t dbSNP:76219783
804 804 a, g dbSNP:762711560
806 806 a, c dbSNP:75971463
809 809 c, t dbSNP:79310911
819 819 g, t dbSNP:763896138
824 824 a, g dbSNP:78315883
827 827 a, c dbSNP:760884054
836 836 a, g dbSNP:775424614
843 843 c, t dbSNP:200390483
844 844 a, g dbSNP:141049110
849 849 a, g dbSNP:768340427
850 850 a, g dbSNP:75184679
868 868 a, g dbSNP:77377571
870 870 a, g dbSNP:760862553
873 873 c, t dbSNP:766618424
875 875 g, t dbSNP:74555752
876 876 c, g dbSNP:566790226
877 877 a, g dbSNP:759712178
883 883 c, t dbSNP:765368182
884 884 a, g dbSNP:138649673
885 885 c, g dbSNP:141367253
888 888 -, acagtca dbSNP:765643940
888 888 a, g dbSNP:764428875
892 892 a, t dbSNP:555463211
893 893 c, g dbSNP:756903057
897 897 g, t dbSNP:780872918
910 910 g, t dbSNP:745471919
933 933 a, g dbSNP:755648468
936 936 a, g dbSNP:779468408
937 937 a, g dbSNP:17857128
938 938 a, c dbSNP:371740577
939 939 g, t dbSNP:762258182
940 940 a, g dbSNP:376240726
943 943 g, t dbSNP:750077795
949 949 c, t dbSNP:769632378
950 950 a, g dbSNP:755705496
953 953 a, t dbSNP:779596970
957 957 c, t dbSNP:753339416
959 959 a, g dbSNP:200648780
966 966 a, g dbSNP:376122649
970 970 g, t dbSNP:778933609
976 976 c, t dbSNP:77391331
980 980 a, c, t dbSNP:747995413
982 982 c, g dbSNP:777655486
985 985 a, g dbSNP:746153565
998 998 a, g dbSNP:770138442
1000 1000 a, g dbSNP:775757158
1004 1004 -, a dbSNP:748406884
1004 1004 a, t dbSNP:763070137
1006 1006 c, t dbSNP:768565639
1018 1018 a, c dbSNP:774950202
1020 1020 a, g dbSNP:768783637
1026 1026 a, g dbSNP:774234566
1028 1028 a, g dbSNP:748746326
1040 1040 c, g, t dbSNP:372632599
1041 1041 a, g dbSNP:761005167
1055 1055 c, t dbSNP:190327832
1065 1065 a, c dbSNP:748144224
1077 1077 a, g dbSNP:200721645
1083 1083 a, g dbSNP:146378238
1084 1084 c, g dbSNP:747450548
1087 1087 g, t dbSNP:771299667
1107 1107 c, t dbSNP:776847998
1108 1108 a, g dbSNP:150363383
1109 1109 c, g dbSNP:183734174
1129 1129 c, g, t dbSNP:775119865
1139 1139 a, g dbSNP:763654606
1172 1172 a, c dbSNP:778729628
1175 1175 a, g dbSNP:377006588
1177 1177 -, tgg dbSNP:766811359
1181 1181 a, g dbSNP:370009090
1192 1192 a, g dbSNP:559536664
1195 1195 a, c dbSNP:141473699
1203 1203 -, g dbSNP:374825738
1289 1289 g, t dbSNP:542131533
1290 1290 g, t dbSNP:375966200
1302 1302 a, g dbSNP:757499643
1327 1327 g, t dbSNP:530824994
1356 1356 c, t dbSNP:550602087
1357 1357 a, g dbSNP:779197062
1380 1380 c, g dbSNP:555426386
1386 1386 c, t dbSNP:192376633
1418 1418 c, t dbSNP:184044774
1442 1442 c, t dbSNP:781732921
1460 1460 c, t dbSNP:533284692
1474 1474 a, g dbSNP:548955070
1480 1480 a, c dbSNP:188678952
1485 1485 a, g dbSNP:150233508
1492 1492 a, g dbSNP:115601793
1505 1505 a, g dbSNP:776130529
1509 1509 c, t dbSNP:377552193
1513 1513 a, t dbSNP:537038741
1549 1549 -, ctta dbSNP:574696297
1569 1569 c, t dbSNP:370311386
1581 1581 a, g dbSNP:746488612
1583 1583 a, g dbSNP:557026437
1584 1584 c, t dbSNP:576999314
1606 1606 a, g dbSNP:768156129
1618 1618 a, g dbSNP:374087970
1621 1621 c, t dbSNP:181458623
1622 1622 a, g dbSNP:572999385
1640 1640 c, t dbSNP:370687538
1644 1644 a, g dbSNP:149475110
1663 1663 c, t dbSNP:575192646

Target ORF information:

RefSeq Version XM_005266525
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005266525

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAG GTTAATCAAA CTGTGGCAGC ATTAAAAACC
AATAATGTGA ATGTCAGTTC CCGGGTACAG TCAACTGCAT TTTTCTCTGG TGACCAAGCT
TCCACTGACA AGGAAGAGGA TTATATTCGT TATGCCCATG GTCTGATATC TGACTACATC
CCTAAAGAAT TAAGTGATGA CTTATCTAAA TACTTAAAGC TTCCAGAACC TTCAGCCTCA
TTGCCAAATC CTCCATCAAA GAAAATAAAG TTATCAGATG AGCCTGTAGA AGCAAAAGAA
GATTACACTA AGTTTAATAC TAAAGATTTG AAGACTGAAA AGATGGCAGC ACAAAGACAG
AAAAGGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71940
Accession Version XM_011535229.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 801bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ribonuclease H2 subunit B isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAG GTTAATCAAA CTGTGGCAGC ATTAAAAACC
AATAATGTGA ATGTCAGTTC CCGGGTACAG TCAACTGCAT TTTTCTCTGG TGACCAAGCT
TCCACTGACA AGGAAGAGGA TTATATTCGT TATGCCCATG GTCTGATATC TGACTACATC
CCTAAAGAAT TAAGTGATGA CTTATCTAAA TACTTAAAGC TTCCAGAACC TTCAGCCTCA
TTGCCAAATC CTCCATCAAA GCTAGGTCTA CCAACTGGTC ATTTTGTGAT GGCAGCACAA
AGACAGAAAA GGGGCAAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011533531.1
CDS322..1122
Misc Feature(1)370..>882(+)
Misc Feature(2)376..546(+)
Translation
Position Chain Variation Link
30 30 c, g dbSNP:544448663
48 48 c, g dbSNP:540984248
94 94 a, c dbSNP:577725827
95 95 a, g dbSNP:564346046
112 112 c, g dbSNP:112702177
114 114 a, g dbSNP:11544097
205 205 c, g dbSNP:553446261
210 210 c, t dbSNP:184573722
246 246 c, g dbSNP:529317356
257 257 c, t dbSNP:549441721
297 297 c, t dbSNP:562869389
313 313 a, g dbSNP:765552524
328 328 a, g dbSNP:531904107
341 341 a, g dbSNP:551573692
360 360 c, t dbSNP:571566229
367 367 c, t dbSNP:763103642
372 372 a, g dbSNP:764223334
373 373 a, t dbSNP:758877268
390 390 a, t dbSNP:778329909
393 393 a, g dbSNP:748052389
400 400 a, g dbSNP:763308917
404 404 c, g dbSNP:771472509
406 406 -, aag dbSNP:776706264
410 410 a, c dbSNP:772952262
413 413 c, t dbSNP:542600186
416 416 a, g dbSNP:377417708
417 417 a, g dbSNP:775880092
419 419 a, c dbSNP:763229293
423 423 a, g dbSNP:764270783
427 427 a, g dbSNP:774427890
448 448 c, t dbSNP:762281569
449 449 a, c dbSNP:79564863
450 450 a, c dbSNP:199839728
453 453 a, t dbSNP:74876702
455 455 c, t dbSNP:575985863
457 457 -, g dbSNP:762857414
474 474 c, t dbSNP:772503413
477 477 a, g dbSNP:35416748
482 482 a, g dbSNP:770080674
485 485 c, t dbSNP:374465686
491 491 c, t dbSNP:537203779
493 493 c, t dbSNP:75326546
494 494 a, g dbSNP:759251667
498 498 a, g dbSNP:141881672
500 500 g, t dbSNP:75325951
501 501 c, g dbSNP:556877869
504 504 c, t dbSNP:368589166
510 510 a, g dbSNP:35595258
514 514 a, g dbSNP:541903338
514 514 -, g dbSNP:758911416
523 523 c, g dbSNP:145465454
524 524 a, g dbSNP:148811739
539 539 g, t dbSNP:78071087
555 555 a, g dbSNP:757200430
561 561 a, g dbSNP:781187392
563 563 -, aggt dbSNP:780704541
568 568 a, g dbSNP:76158094
571 571 c, g dbSNP:143523027
572 572 g, t dbSNP:747061909
578 578 a, g dbSNP:77931005
584 584 c, g dbSNP:777149786
588 588 -, c dbSNP:781518432
589 589 c, t dbSNP:746338829
590 590 c, g, t dbSNP:770203126
594 594 g, t dbSNP:374098961
598 598 c, g dbSNP:763646959
609 609 c, g dbSNP:774041308
625 625 a, g dbSNP:761380890
629 629 a, g dbSNP:767086382
635 635 a, c dbSNP:201078944
638 638 a, c dbSNP:755936337
645 645 a, g dbSNP:760734846
669 669 c, t dbSNP:766196712
673 673 c, g dbSNP:753716315
677 677 a, g dbSNP:786205483
681 681 c, t dbSNP:759273327
682 682 a, g dbSNP:764980431
686 686 g, t dbSNP:751788888
691 691 a, g dbSNP:757415855
696 696 c, t dbSNP:781310346
697 697 a, g dbSNP:569343532
708 708 a, g dbSNP:756682554
715 715 c, g dbSNP:780398368
716 716 c, g dbSNP:749750512
724 724 a, g dbSNP:769173136
728 728 a, c dbSNP:199935006
733 733 c, t dbSNP:78705382
749 749 a, t dbSNP:200535256
750 750 c, g dbSNP:754434417
761 761 a, c dbSNP:746633003
766 766 c, g dbSNP:770355902
776 776 a, g dbSNP:146451037
777 777 c, t dbSNP:759529625
779 779 a, g dbSNP:769721789
786 786 c, t dbSNP:202232001
789 789 c, t dbSNP:775300215
797 797 g, t dbSNP:76219783
804 804 a, g dbSNP:762711560
806 806 a, c dbSNP:75971463
809 809 c, t dbSNP:79310911
819 819 g, t dbSNP:763896138
824 824 a, g dbSNP:78315883
827 827 a, c dbSNP:760884054
836 836 a, g dbSNP:775424614
843 843 c, t dbSNP:200390483
844 844 a, g dbSNP:141049110
849 849 a, g dbSNP:768340427
850 850 a, g dbSNP:75184679
868 868 a, g dbSNP:77377571
870 870 a, g dbSNP:760862553
873 873 c, t dbSNP:766618424
875 875 g, t dbSNP:74555752
876 876 c, g dbSNP:566790226
877 877 a, g dbSNP:759712178
883 883 c, t dbSNP:765368182
884 884 a, g dbSNP:138649673
885 885 c, g dbSNP:141367253
888 888 -, acagtca dbSNP:765643940
888 888 a, g dbSNP:764428875
892 892 a, t dbSNP:555463211
893 893 c, g dbSNP:756903057
897 897 g, t dbSNP:780872918
910 910 g, t dbSNP:745471919
933 933 a, g dbSNP:755648468
936 936 a, g dbSNP:779468408
937 937 a, g dbSNP:17857128
938 938 a, c dbSNP:371740577
939 939 g, t dbSNP:762258182
940 940 a, g dbSNP:376240726
943 943 g, t dbSNP:750077795
949 949 c, t dbSNP:769632378
950 950 a, g dbSNP:755705496
953 953 a, t dbSNP:779596970
957 957 c, t dbSNP:753339416
959 959 a, g dbSNP:200648780
966 966 a, g dbSNP:376122649
970 970 g, t dbSNP:778933609
976 976 c, t dbSNP:77391331
980 980 a, c, t dbSNP:747995413
982 982 c, g dbSNP:777655486
985 985 a, g dbSNP:746153565
998 998 a, g dbSNP:770138442
1000 1000 a, g dbSNP:775757158
1004 1004 -, a dbSNP:748406884
1004 1004 a, t dbSNP:763070137
1006 1006 c, t dbSNP:768565639
1018 1018 a, c dbSNP:774950202
1020 1020 a, g dbSNP:768783637
1026 1026 a, g dbSNP:774234566
1028 1028 a, g dbSNP:748746326
1040 1040 c, g, t dbSNP:372632599
1041 1041 a, g dbSNP:761005167
1055 1055 c, t dbSNP:190327832
1118 1118 a, c dbSNP:778729628
1121 1121 a, g dbSNP:377006588
1123 1123 -, tgg dbSNP:766811359
1127 1127 a, g dbSNP:370009090
1138 1138 a, g dbSNP:559536664
1141 1141 a, c dbSNP:141473699
1149 1149 -, g dbSNP:374825738
1235 1235 g, t dbSNP:542131533
1236 1236 g, t dbSNP:375966200
1248 1248 a, g dbSNP:757499643
1273 1273 g, t dbSNP:530824994
1302 1302 c, t dbSNP:550602087
1303 1303 a, g dbSNP:779197062
1326 1326 c, g dbSNP:555426386
1332 1332 c, t dbSNP:192376633
1364 1364 c, t dbSNP:184044774
1388 1388 c, t dbSNP:781732921
1406 1406 c, t dbSNP:533284692
1420 1420 a, g dbSNP:548955070
1426 1426 a, c dbSNP:188678952
1431 1431 a, g dbSNP:150233508
1438 1438 a, g dbSNP:115601793
1451 1451 a, g dbSNP:776130529
1455 1455 c, t dbSNP:377552193
1459 1459 a, t dbSNP:537038741
1495 1495 -, ctta dbSNP:574696297
1515 1515 c, t dbSNP:370311386
1527 1527 a, g dbSNP:746488612
1529 1529 a, g dbSNP:557026437
1530 1530 c, t dbSNP:576999314
1552 1552 a, g dbSNP:768156129
1564 1564 a, g dbSNP:374087970
1567 1567 c, t dbSNP:181458623
1568 1568 a, g dbSNP:572999385
1586 1586 c, t dbSNP:370687538
1590 1590 a, g dbSNP:149475110
1609 1609 c, t dbSNP:575192646

Target ORF information:

RefSeq Version XM_011535229
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535229

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAG GTTAATCAAA CTGTGGCAGC ATTAAAAACC
AATAATGTGA ATGTCAGTTC CCGGGTACAG TCAACTGCAT TTTTCTCTGG TGACCAAGCT
TCCACTGACA AGGAAGAGGA TTATATTCGT TATGCCCATG GTCTGATATC TGACTACATC
CCTAAAGAAT TAAGTGATGA CTTATCTAAA TACTTAAAGC TTCCAGAACC TTCAGCCTCA
TTGCCAAATC CTCCATCAAA GCTAGGTCTA CCAACTGGTC ATTTTGTGAT GGCAGCACAA
AGACAGAAAA GGGGCAAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71941
Accession Version XM_011535230.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ribonuclease H2 subunit B isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAG GTTAATCAAA CTGTGGCAGC ATTAAAAACC
AATAATGTGA ATGTCAGTTC CCGGGTACAG TCAACTGCAT TTTTCTCTGG TGACCAAGCT
TCCACTGACA AGGAAGAGGA TTATATTCGT TATGCCCATG GTCTGATATC TGACTACATC
CCTAAAGAAT TAAGTGATGA CTTATCTAAA TACTTAAAGC TTCCAGAACC TTCAGCCTCA
TTGCCAAATC CTCCATCAAA GGCTGCTACC CACAGCTCCT CTGCAGCTGG TATGTTTATC
TCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011533532.1
CDS322..1107
Misc Feature(1)370..>882(+)
Misc Feature(2)376..546(+)
Translation
Position Chain Variation Link
30 30 c, g dbSNP:544448663
48 48 c, g dbSNP:540984248
94 94 a, c dbSNP:577725827
95 95 a, g dbSNP:564346046
112 112 c, g dbSNP:112702177
114 114 a, g dbSNP:11544097
205 205 c, g dbSNP:553446261
210 210 c, t dbSNP:184573722
246 246 c, g dbSNP:529317356
257 257 c, t dbSNP:549441721
297 297 c, t dbSNP:562869389
313 313 a, g dbSNP:765552524
328 328 a, g dbSNP:531904107
341 341 a, g dbSNP:551573692
360 360 c, t dbSNP:571566229
367 367 c, t dbSNP:763103642
372 372 a, g dbSNP:764223334
373 373 a, t dbSNP:758877268
390 390 a, t dbSNP:778329909
393 393 a, g dbSNP:748052389
400 400 a, g dbSNP:763308917
404 404 c, g dbSNP:771472509
406 406 -, aag dbSNP:776706264
410 410 a, c dbSNP:772952262
413 413 c, t dbSNP:542600186
416 416 a, g dbSNP:377417708
417 417 a, g dbSNP:775880092
419 419 a, c dbSNP:763229293
423 423 a, g dbSNP:764270783
427 427 a, g dbSNP:774427890
448 448 c, t dbSNP:762281569
449 449 a, c dbSNP:79564863
450 450 a, c dbSNP:199839728
453 453 a, t dbSNP:74876702
455 455 c, t dbSNP:575985863
457 457 -, g dbSNP:762857414
474 474 c, t dbSNP:772503413
477 477 a, g dbSNP:35416748
482 482 a, g dbSNP:770080674
485 485 c, t dbSNP:374465686
491 491 c, t dbSNP:537203779
493 493 c, t dbSNP:75326546
494 494 a, g dbSNP:759251667
498 498 a, g dbSNP:141881672
500 500 g, t dbSNP:75325951
501 501 c, g dbSNP:556877869
504 504 c, t dbSNP:368589166
510 510 a, g dbSNP:35595258
514 514 a, g dbSNP:541903338
514 514 -, g dbSNP:758911416
523 523 c, g dbSNP:145465454
524 524 a, g dbSNP:148811739
539 539 g, t dbSNP:78071087
555 555 a, g dbSNP:757200430
561 561 a, g dbSNP:781187392
563 563 -, aggt dbSNP:780704541
568 568 a, g dbSNP:76158094
571 571 c, g dbSNP:143523027
572 572 g, t dbSNP:747061909
578 578 a, g dbSNP:77931005
584 584 c, g dbSNP:777149786
588 588 -, c dbSNP:781518432
589 589 c, t dbSNP:746338829
590 590 c, g, t dbSNP:770203126
594 594 g, t dbSNP:374098961
598 598 c, g dbSNP:763646959
609 609 c, g dbSNP:774041308
625 625 a, g dbSNP:761380890
629 629 a, g dbSNP:767086382
635 635 a, c dbSNP:201078944
638 638 a, c dbSNP:755936337
645 645 a, g dbSNP:760734846
669 669 c, t dbSNP:766196712
673 673 c, g dbSNP:753716315
677 677 a, g dbSNP:786205483
681 681 c, t dbSNP:759273327
682 682 a, g dbSNP:764980431
686 686 g, t dbSNP:751788888
691 691 a, g dbSNP:757415855
696 696 c, t dbSNP:781310346
697 697 a, g dbSNP:569343532
708 708 a, g dbSNP:756682554
715 715 c, g dbSNP:780398368
716 716 c, g dbSNP:749750512
724 724 a, g dbSNP:769173136
728 728 a, c dbSNP:199935006
733 733 c, t dbSNP:78705382
749 749 a, t dbSNP:200535256
750 750 c, g dbSNP:754434417
761 761 a, c dbSNP:746633003
766 766 c, g dbSNP:770355902
776 776 a, g dbSNP:146451037
777 777 c, t dbSNP:759529625
779 779 a, g dbSNP:769721789
786 786 c, t dbSNP:202232001
789 789 c, t dbSNP:775300215
797 797 g, t dbSNP:76219783
804 804 a, g dbSNP:762711560
806 806 a, c dbSNP:75971463
809 809 c, t dbSNP:79310911
819 819 g, t dbSNP:763896138
824 824 a, g dbSNP:78315883
827 827 a, c dbSNP:760884054
836 836 a, g dbSNP:775424614
843 843 c, t dbSNP:200390483
844 844 a, g dbSNP:141049110
849 849 a, g dbSNP:768340427
850 850 a, g dbSNP:75184679
868 868 a, g dbSNP:77377571
870 870 a, g dbSNP:760862553
873 873 c, t dbSNP:766618424
875 875 g, t dbSNP:74555752
876 876 c, g dbSNP:566790226
877 877 a, g dbSNP:759712178
883 883 c, t dbSNP:765368182
884 884 a, g dbSNP:138649673
885 885 c, g dbSNP:141367253
888 888 -, acagtca dbSNP:765643940
888 888 a, g dbSNP:764428875
892 892 a, t dbSNP:555463211
893 893 c, g dbSNP:756903057
897 897 g, t dbSNP:780872918
910 910 g, t dbSNP:745471919
933 933 a, g dbSNP:755648468
936 936 a, g dbSNP:779468408
937 937 a, g dbSNP:17857128
938 938 a, c dbSNP:371740577
939 939 g, t dbSNP:762258182
940 940 a, g dbSNP:376240726
943 943 g, t dbSNP:750077795
949 949 c, t dbSNP:769632378
950 950 a, g dbSNP:755705496
953 953 a, t dbSNP:779596970
957 957 c, t dbSNP:753339416
959 959 a, g dbSNP:200648780
966 966 a, g dbSNP:376122649
970 970 g, t dbSNP:778933609
976 976 c, t dbSNP:77391331
980 980 a, c, t dbSNP:747995413
982 982 c, g dbSNP:777655486
985 985 a, g dbSNP:746153565
998 998 a, g dbSNP:770138442
1000 1000 a, g dbSNP:775757158
1004 1004 -, a dbSNP:748406884
1004 1004 a, t dbSNP:763070137
1006 1006 c, t dbSNP:768565639
1018 1018 a, c dbSNP:774950202
1020 1020 a, g dbSNP:768783637
1026 1026 a, g dbSNP:774234566
1028 1028 a, g dbSNP:748746326
1040 1040 c, g, t dbSNP:372632599
1041 1041 a, g dbSNP:761005167
1055 1055 c, t dbSNP:190327832
1100 1100 a, t dbSNP:114777768
1103 1103 c, g dbSNP:182887683

Target ORF information:

RefSeq Version XM_011535230
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535230

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAG GTTAATCAAA CTGTGGCAGC ATTAAAAACC
AATAATGTGA ATGTCAGTTC CCGGGTACAG TCAACTGCAT TTTTCTCTGG TGACCAAGCT
TCCACTGACA AGGAAGAGGA TTATATTCGT TATGCCCATG GTCTGATATC TGACTACATC
CCTAAAGAAT TAAGTGATGA CTTATCTAAA TACTTAAAGC TTCCAGAACC TTCAGCCTCA
TTGCCAAATC CTCCATCAAA GGCTGCTACC CACAGCTCCT CTGCAGCTGG TATGTTTATC
TCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71942
Accession Version XM_011535231.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 780bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ribonuclease H2 subunit B isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAG GTTAATCAAA CTGTGGCAGC ATTAAAAACC
AATAATGTGA ATGTCAGTTC CCGGGTACAG TCAACTGCAT TTTTCTCTGG TGACCAAGCT
TCCACTGACA AGGAAGAGGA TTATATTCGT TATGCCCATG GTCTGATATC TGACTACATC
CCTAAAGAAT TAAGTGATGA CTTATCTAAA TACTTAAAGC TTCCAGAACC TTCAGCCTCA
TTGCCAAATC CTCCATCAAA GATCCAGAGA ATGGAGAAGC CAATCATAAA CCACACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011533533.1
CDS322..1101
Misc Feature(1)370..>882(+)
Misc Feature(2)376..546(+)
Translation
Position Chain Variation Link
30 30 c, g dbSNP:544448663
48 48 c, g dbSNP:540984248
94 94 a, c dbSNP:577725827
95 95 a, g dbSNP:564346046
112 112 c, g dbSNP:112702177
114 114 a, g dbSNP:11544097
205 205 c, g dbSNP:553446261
210 210 c, t dbSNP:184573722
246 246 c, g dbSNP:529317356
257 257 c, t dbSNP:549441721
297 297 c, t dbSNP:562869389
313 313 a, g dbSNP:765552524
328 328 a, g dbSNP:531904107
341 341 a, g dbSNP:551573692
360 360 c, t dbSNP:571566229
367 367 c, t dbSNP:763103642
372 372 a, g dbSNP:764223334
373 373 a, t dbSNP:758877268
390 390 a, t dbSNP:778329909
393 393 a, g dbSNP:748052389
400 400 a, g dbSNP:763308917
404 404 c, g dbSNP:771472509
406 406 -, aag dbSNP:776706264
410 410 a, c dbSNP:772952262
413 413 c, t dbSNP:542600186
416 416 a, g dbSNP:377417708
417 417 a, g dbSNP:775880092
419 419 a, c dbSNP:763229293
423 423 a, g dbSNP:764270783
427 427 a, g dbSNP:774427890
448 448 c, t dbSNP:762281569
449 449 a, c dbSNP:79564863
450 450 a, c dbSNP:199839728
453 453 a, t dbSNP:74876702
455 455 c, t dbSNP:575985863
457 457 -, g dbSNP:762857414
474 474 c, t dbSNP:772503413
477 477 a, g dbSNP:35416748
482 482 a, g dbSNP:770080674
485 485 c, t dbSNP:374465686
491 491 c, t dbSNP:537203779
493 493 c, t dbSNP:75326546
494 494 a, g dbSNP:759251667
498 498 a, g dbSNP:141881672
500 500 g, t dbSNP:75325951
501 501 c, g dbSNP:556877869
504 504 c, t dbSNP:368589166
510 510 a, g dbSNP:35595258
514 514 a, g dbSNP:541903338
514 514 -, g dbSNP:758911416
523 523 c, g dbSNP:145465454
524 524 a, g dbSNP:148811739
539 539 g, t dbSNP:78071087
555 555 a, g dbSNP:757200430
561 561 a, g dbSNP:781187392
563 563 -, aggt dbSNP:780704541
568 568 a, g dbSNP:76158094
571 571 c, g dbSNP:143523027
572 572 g, t dbSNP:747061909
578 578 a, g dbSNP:77931005
584 584 c, g dbSNP:777149786
588 588 -, c dbSNP:781518432
589 589 c, t dbSNP:746338829
590 590 c, g, t dbSNP:770203126
594 594 g, t dbSNP:374098961
598 598 c, g dbSNP:763646959
609 609 c, g dbSNP:774041308
625 625 a, g dbSNP:761380890
629 629 a, g dbSNP:767086382
635 635 a, c dbSNP:201078944
638 638 a, c dbSNP:755936337
645 645 a, g dbSNP:760734846
669 669 c, t dbSNP:766196712
673 673 c, g dbSNP:753716315
677 677 a, g dbSNP:786205483
681 681 c, t dbSNP:759273327
682 682 a, g dbSNP:764980431
686 686 g, t dbSNP:751788888
691 691 a, g dbSNP:757415855
696 696 c, t dbSNP:781310346
697 697 a, g dbSNP:569343532
708 708 a, g dbSNP:756682554
715 715 c, g dbSNP:780398368
716 716 c, g dbSNP:749750512
724 724 a, g dbSNP:769173136
728 728 a, c dbSNP:199935006
733 733 c, t dbSNP:78705382
749 749 a, t dbSNP:200535256
750 750 c, g dbSNP:754434417
761 761 a, c dbSNP:746633003
766 766 c, g dbSNP:770355902
776 776 a, g dbSNP:146451037
777 777 c, t dbSNP:759529625
779 779 a, g dbSNP:769721789
786 786 c, t dbSNP:202232001
789 789 c, t dbSNP:775300215
797 797 g, t dbSNP:76219783
804 804 a, g dbSNP:762711560
806 806 a, c dbSNP:75971463
809 809 c, t dbSNP:79310911
819 819 g, t dbSNP:763896138
824 824 a, g dbSNP:78315883
827 827 a, c dbSNP:760884054
836 836 a, g dbSNP:775424614
843 843 c, t dbSNP:200390483
844 844 a, g dbSNP:141049110
849 849 a, g dbSNP:768340427
850 850 a, g dbSNP:75184679
868 868 a, g dbSNP:77377571
870 870 a, g dbSNP:760862553
873 873 c, t dbSNP:766618424
875 875 g, t dbSNP:74555752
876 876 c, g dbSNP:566790226
877 877 a, g dbSNP:759712178
883 883 c, t dbSNP:765368182
884 884 a, g dbSNP:138649673
885 885 c, g dbSNP:141367253
888 888 -, acagtca dbSNP:765643940
888 888 a, g dbSNP:764428875
892 892 a, t dbSNP:555463211
893 893 c, g dbSNP:756903057
897 897 g, t dbSNP:780872918
910 910 g, t dbSNP:745471919
933 933 a, g dbSNP:755648468
936 936 a, g dbSNP:779468408
937 937 a, g dbSNP:17857128
938 938 a, c dbSNP:371740577
939 939 g, t dbSNP:762258182
940 940 a, g dbSNP:376240726
943 943 g, t dbSNP:750077795
949 949 c, t dbSNP:769632378
950 950 a, g dbSNP:755705496
953 953 a, t dbSNP:779596970
957 957 c, t dbSNP:753339416
959 959 a, g dbSNP:200648780
966 966 a, g dbSNP:376122649
970 970 g, t dbSNP:778933609
976 976 c, t dbSNP:77391331
980 980 a, c, t dbSNP:747995413
982 982 c, g dbSNP:777655486
985 985 a, g dbSNP:746153565
998 998 a, g dbSNP:770138442
1000 1000 a, g dbSNP:775757158
1004 1004 -, a dbSNP:748406884
1004 1004 a, t dbSNP:763070137
1006 1006 c, t dbSNP:768565639
1018 1018 a, c dbSNP:774950202
1020 1020 a, g dbSNP:768783637
1026 1026 a, g dbSNP:774234566
1028 1028 a, g dbSNP:748746326
1040 1040 c, g, t dbSNP:372632599
1041 1041 a, g dbSNP:761005167
1055 1055 c, t dbSNP:190327832
1107 1107 a, g dbSNP:200841932
1127 1127 c, t dbSNP:114308005
1152 1152 a, t dbSNP:565102475
1161 1161 c, t dbSNP:572427953
1173 1173 a, g dbSNP:189037251
1255 1255 c, t dbSNP:115028119
1277 1277 c, g dbSNP:530258187
1330 1330 -, t dbSNP:199834080
1335 1335 -, t dbSNP:778431579
1355 1355 -, ggt dbSNP:752791100
1370 1370 a, c dbSNP:572711986
1380 1380 a, t dbSNP:770877976
1388 1388 a, g dbSNP:774193815
1391 1391 g, t dbSNP:550395423
1415 1415 a, g dbSNP:747105634
1416 1416 -, tgtt dbSNP:200928328
1428 1428 c, t dbSNP:768678060
1431 1431 a, g dbSNP:563902607
1447 1447 -, a dbSNP:202193335
1464 1464 g, t dbSNP:765577914
1493 1493 g, t dbSNP:565776681
1506 1506 a, g dbSNP:776864716
1517 1517 a, g dbSNP:762059702
1536 1536 c, t dbSNP:193139983
1553 1553 c, t dbSNP:765591512
1624 1624 c, t dbSNP:146666465
1636 1636 a, g dbSNP:77007792
1639 1639 c, g dbSNP:574880985
1641 1641 g, t dbSNP:752062014
1645 1645 a, g dbSNP:140360072
1714 1714 a, g dbSNP:184401803
1766 1766 c, t dbSNP:755518810
1850 1850 c, t dbSNP:187687735
1865 1865 c, t dbSNP:17590275
1882 1882 a, g dbSNP:730076
1963 1963 c, t dbSNP:576202690
1972 1972 c, t dbSNP:114571412
1987 1987 -, aaaaac dbSNP:148092850
2038 2038 c, g dbSNP:368713705
2055 2055 a, g dbSNP:544930710
2059 2059 a, g dbSNP:777449927
2074 2074 c, g dbSNP:184031729
2081 2081 a, g dbSNP:150379727
2118 2118 c, t dbSNP:541407024
2153 2153 a, t dbSNP:372279045
2158 2158 a, t dbSNP:561313574
2170 2170 c, g dbSNP:574965533
2231 2231 c, t dbSNP:6561602
2234 2234 c, t dbSNP:563836876
2243 2243 a, g dbSNP:532762019
2255 2255 a, g dbSNP:144984924
2258 2258 a, g dbSNP:745736881
2329 2329 c, g dbSNP:540846580
2386 2386 a, g dbSNP:188715244
2392 2392 a, g dbSNP:528821085
2395 2395 a, g dbSNP:546984403
2419 2419 -, ta dbSNP:758785216
2507 2507 g, t dbSNP:566949815
2539 2539 a, g dbSNP:776812972
2546 2546 a, g dbSNP:536466791
2565 2565 c, t dbSNP:549489425
2573 2573 a, c dbSNP:770122096
2585 2585 a, g dbSNP:569707229
2586 2586 a, g dbSNP:181448635
2611 2611 c, t dbSNP:149079392
2628 2628 c, g dbSNP:773612478
2642 2642 a, g dbSNP:750616589
2667 2667 a, c dbSNP:185359469
2695 2695 a, g dbSNP:560870632
2732 2732 c, t dbSNP:534946244
2737 2737 a, t dbSNP:756473272
2847 2847 a, g dbSNP:529839500
2857 2857 a, c dbSNP:574643525
2859 2859 c, t dbSNP:76130067
2878 2878 g, t dbSNP:766591750
2899 2899 -, a dbSNP:778945479
2904 2904 a, c dbSNP:554989175
2932 2932 a, g dbSNP:190605796

Target ORF information:

RefSeq Version XM_011535231
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535231

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAG GTTAATCAAA CTGTGGCAGC ATTAAAAACC
AATAATGTGA ATGTCAGTTC CCGGGTACAG TCAACTGCAT TTTTCTCTGG TGACCAAGCT
TCCACTGACA AGGAAGAGGA TTATATTCGT TATGCCCATG GTCTGATATC TGACTACATC
CCTAAAGAAT TAAGTGATGA CTTATCTAAA TACTTAAAGC TTCCAGAACC TTCAGCCTCA
TTGCCAAATC CTCCATCAAA GATCCAGAGA ATGGAGAAGC CAATCATAAA CCACACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71943
Accession Version XM_011535232.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 777bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ribonuclease H2 subunit B isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTGTCTAC AGCAGCTGTT TGAAGTAAAA GTTTTCAAGG AAAAACACCA TTCTTGGTTT 
ATAAATCAAT CAGTTCAATC AGGAGGTCTT CTCCATTTTG CCACACCTGT GGATCCTCTA
TTTCTGCTTC TCCACTACCT CATAAAGGCT GATAAGGAGG GGAAGTTTCA GCCCCTTGAT
CAAGTTGTGG TGGATAACGT GTTTCCAAAT TGCATCTTGT TGCTGAAACT TCCTGGACTT
GAGAAGTTAC TTCATCATGT GACAGAGGAA AAAGGTAATC CAGAAATAGA CAACAAGAAA
TATTACAAGT ACAGCAAAGA GAAGACATTA AAGTGGCTGG AAAAAAAGGT TAATCAAACT
GTGGCAGCAT TAAAAACCAA TAATGTGAAT GTCAGTTCCC GGGTACAGTC AACTGCATTT
TTCTCTGGTG ACCAAGCTTC CACTGACAAG GAAGAGGATT ATATTCGTTA TGCCCATGGT
CTGATATCTG ACTACATCCC TAAAGAATTA AGTGATGACT TATCTAAATA CTTAAAGCTT
CCAGAACCTT CAGCCTCATT GCCAAATCCT CCATCAAAGA AAATAAAGTT ATCAGATGAG
CCTGTAGAAG CAAAAGAAGA TTACACTAAG TTTAATACTA AAGATTTGAA GACTGAAAAG
AAAAATAGCA AAATGACTGC AGCTCAGAAG GCTTTGGCTA AAGTTGACAA GAGTGGAATG
AAAAGTATTG ATACCTTTTT TGGGGTAAAA AATAAAAAAA AAATTGGAAA GGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011533534.1
CDS119..895
Misc Feature(1)128..862(+)
Misc Feature(2)143..181(+)
Translation
Position Chain Variation Link
5 5 a, g dbSNP:754503022
11 11 a, t dbSNP:778329909
14 14 a, g dbSNP:748052389
21 21 a, g dbSNP:763308917
25 25 c, g dbSNP:771472509
27 27 -, aag dbSNP:776706264
31 31 a, c dbSNP:772952262
34 34 c, t dbSNP:542600186
37 37 a, g dbSNP:377417708
38 38 a, g dbSNP:775880092
40 40 a, c dbSNP:763229293
44 44 a, g dbSNP:764270783
48 48 a, g dbSNP:774427890
69 69 c, t dbSNP:762281569
70 70 a, c dbSNP:79564863
71 71 a, c dbSNP:199839728
74 74 a, t dbSNP:74876702
76 76 c, t dbSNP:575985863
78 78 -, g dbSNP:762857414
83 83 a, g dbSNP:761921568
109 109 c, t dbSNP:772503413
112 112 a, g dbSNP:35416748
117 117 a, g dbSNP:770080674
120 120 c, t dbSNP:374465686
126 126 c, t dbSNP:537203779
128 128 c, t dbSNP:75326546
129 129 a, g dbSNP:759251667
133 133 a, g dbSNP:141881672
135 135 g, t dbSNP:75325951
136 136 c, g dbSNP:556877869
139 139 c, t dbSNP:368589166
145 145 a, g dbSNP:35595258
149 149 a, g dbSNP:541903338
149 149 -, g dbSNP:758911416
158 158 c, g dbSNP:145465454
159 159 a, g dbSNP:148811739
174 174 g, t dbSNP:78071087
190 190 a, g dbSNP:757200430
196 196 a, g dbSNP:781187392
198 198 -, aggt dbSNP:780704541
203 203 a, g dbSNP:76158094
206 206 c, g dbSNP:143523027
207 207 g, t dbSNP:747061909
213 213 a, g dbSNP:77931005
219 219 c, g dbSNP:777149786
223 223 -, c dbSNP:781518432
224 224 c, t dbSNP:746338829
225 225 c, g, t dbSNP:770203126
229 229 g, t dbSNP:374098961
233 233 c, g dbSNP:763646959
244 244 c, g dbSNP:774041308
260 260 a, g dbSNP:761380890
264 264 a, g dbSNP:767086382
270 270 a, c dbSNP:201078944
273 273 a, c dbSNP:755936337
280 280 a, g dbSNP:760734846
304 304 c, t dbSNP:766196712
308 308 c, g dbSNP:753716315
312 312 a, g dbSNP:786205483
316 316 c, t dbSNP:759273327
317 317 a, g dbSNP:764980431
321 321 g, t dbSNP:751788888
326 326 a, g dbSNP:757415855
331 331 c, t dbSNP:781310346
332 332 a, g dbSNP:569343532
343 343 a, g dbSNP:756682554
350 350 c, g dbSNP:780398368
351 351 c, g dbSNP:749750512
359 359 a, g dbSNP:769173136
363 363 a, c dbSNP:199935006
368 368 c, t dbSNP:78705382
384 384 a, t dbSNP:200535256
385 385 c, g dbSNP:754434417
396 396 a, c dbSNP:746633003
401 401 c, g dbSNP:770355902
411 411 a, g dbSNP:146451037
412 412 c, t dbSNP:759529625
414 414 a, g dbSNP:769721789
421 421 c, t dbSNP:202232001
424 424 c, t dbSNP:775300215
432 432 g, t dbSNP:76219783
439 439 a, g dbSNP:762711560
441 441 a, c dbSNP:75971463
444 444 c, t dbSNP:79310911
454 454 g, t dbSNP:763896138
459 459 a, g dbSNP:78315883
462 462 a, c dbSNP:760884054
471 471 a, g dbSNP:775424614
478 478 c, t dbSNP:200390483
479 479 a, g dbSNP:141049110
484 484 a, g dbSNP:768340427
485 485 a, g dbSNP:75184679
503 503 a, g dbSNP:77377571
505 505 a, g dbSNP:760862553
508 508 c, t dbSNP:766618424
510 510 g, t dbSNP:74555752
511 511 c, g dbSNP:566790226
512 512 a, g dbSNP:759712178
518 518 c, t dbSNP:765368182
519 519 a, g dbSNP:138649673
520 520 c, g dbSNP:141367253
523 523 -, acagtca dbSNP:765643940
523 523 a, g dbSNP:764428875
527 527 a, t dbSNP:555463211
528 528 c, g dbSNP:756903057
532 532 g, t dbSNP:780872918
545 545 g, t dbSNP:745471919
568 568 a, g dbSNP:755648468
571 571 a, g dbSNP:779468408
572 572 a, g dbSNP:17857128
573 573 a, c dbSNP:371740577
574 574 g, t dbSNP:762258182
575 575 a, g dbSNP:376240726
578 578 g, t dbSNP:750077795
584 584 c, t dbSNP:769632378
585 585 a, g dbSNP:755705496
588 588 a, t dbSNP:779596970
592 592 c, t dbSNP:753339416
594 594 a, g dbSNP:200648780
601 601 a, g dbSNP:376122649
605 605 g, t dbSNP:778933609
611 611 c, t dbSNP:77391331
615 615 a, c, t dbSNP:747995413
617 617 c, g dbSNP:777655486
620 620 a, g dbSNP:746153565
633 633 a, g dbSNP:770138442
635 635 a, g dbSNP:775757158
639 639 -, a dbSNP:748406884
639 639 a, t dbSNP:763070137
641 641 c, t dbSNP:768565639
653 653 a, c dbSNP:774950202
655 655 a, g dbSNP:768783637
661 661 a, g dbSNP:774234566
663 663 a, g dbSNP:748746326
675 675 c, g, t dbSNP:372632599
676 676 a, g dbSNP:761005167
690 690 c, t dbSNP:190327832
700 700 a, c dbSNP:748144224
712 712 a, g dbSNP:200721645
718 718 a, g dbSNP:146378238
719 719 c, g dbSNP:747450548
722 722 g, t dbSNP:771299667
742 742 c, t dbSNP:776847998
743 743 a, g dbSNP:150363383
744 744 c, g dbSNP:183734174
764 764 c, g, t dbSNP:775119865
774 774 a, g dbSNP:763654606
780 780 a, g dbSNP:761906095
781 781 a, g dbSNP:559000645
786 786 a, g dbSNP:200802557
790 790 a, g dbSNP:750279427
799 799 a, c dbSNP:760409807
804 804 a, g dbSNP:766157957
809 809 a, g dbSNP:752930177
810 810 c, t dbSNP:758605855
815 815 g, t dbSNP:144408326
824 824 a, g dbSNP:201190805
844 844 c, t dbSNP:757835049
845 845 a, g dbSNP:781574666
851 851 a, g dbSNP:370507842
855 855 a, t dbSNP:770055331
858 858 c, t dbSNP:780504013
860 860 a, g dbSNP:749003546
863 863 -, t dbSNP:768221761
870 870 a, c dbSNP:768415436
871 871 -, a, aa dbSNP:200320729
871 871 a, t dbSNP:112937854
872 872 -, a dbSNP:761386077
872 872 a, t dbSNP:774043353
881 881 -, a dbSNP:75254367
881 881 a, g dbSNP:761204571
884 884 -, ttat dbSNP:772980492
884 884 a, g dbSNP:771560732
907 907 c, t dbSNP:773052815
928 928 g, t dbSNP:529254634
935 935 a, t dbSNP:766145608
944 944 a, g dbSNP:753627853
948 948 c, t dbSNP:549023975
949 949 g, t dbSNP:569240707
958 958 c, g, t dbSNP:751735556
962 962 a, g dbSNP:781270130
971 971 a, t dbSNP:750903767
981 981 g, t dbSNP:756627118
997 997 -, c dbSNP:763452841
1005 1005 a, g dbSNP:761886682
1041 1041 c, t dbSNP:780358371
1141 1141 a, g dbSNP:147825612
1146 1146 g, t dbSNP:747397214
1168 1168 c, t dbSNP:551658315

Target ORF information:

RefSeq Version XM_011535232
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535232

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTGTCTAC AGCAGCTGTT TGAAGTAAAA GTTTTCAAGG AAAAACACCA TTCTTGGTTT 
ATAAATCAAT CAGTTCAATC AGGAGGTCTT CTCCATTTTG CCACACCTGT GGATCCTCTA
TTTCTGCTTC TCCACTACCT CATAAAGGCT GATAAGGAGG GGAAGTTTCA GCCCCTTGAT
CAAGTTGTGG TGGATAACGT GTTTCCAAAT TGCATCTTGT TGCTGAAACT TCCTGGACTT
GAGAAGTTAC TTCATCATGT GACAGAGGAA AAAGGTAATC CAGAAATAGA CAACAAGAAA
TATTACAAGT ACAGCAAAGA GAAGACATTA AAGTGGCTGG AAAAAAAGGT TAATCAAACT
GTGGCAGCAT TAAAAACCAA TAATGTGAAT GTCAGTTCCC GGGTACAGTC AACTGCATTT
TTCTCTGGTG ACCAAGCTTC CACTGACAAG GAAGAGGATT ATATTCGTTA TGCCCATGGT
CTGATATCTG ACTACATCCC TAAAGAATTA AGTGATGACT TATCTAAATA CTTAAAGCTT
CCAGAACCTT CAGCCTCATT GCCAAATCCT CCATCAAAGA AAATAAAGTT ATCAGATGAG
CCTGTAGAAG CAAAAGAAGA TTACACTAAG TTTAATACTA AAGATTTGAA GACTGAAAAG
AAAAATAGCA AAATGACTGC AGCTCAGAAG GCTTTGGCTA AAGTTGACAA GAGTGGAATG
AAAAGTATTG ATACCTTTTT TGGGGTAAAA AATAAAAAAA AAATTGGAAA GGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71944
Accession Version XM_011535233.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 531bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ribonuclease H2 subunit B isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGGGCAGTT TTCATTTCCC ATCAAAAGGT AATCCAGAAA TAGACAACAA GAAATATTAC 
AAGTACAGCA AAGAGAAGAC ATTAAAGTGG CTGGAAAAAA AGGTTAATCA AACTGTGGCA
GCATTAAAAA CCAATAATGT GAATGTCAGT TCCCGGGTAC AGTCAACTGC ATTTTTCTCT
GGTGACCAAG CTTCCACTGA CAAGGAAGAG GATTATATTC GTTATGCCCA TGGTCTGATA
TCTGACTACA TCCCTAAAGA ATTAAGTGAT GACTTATCTA AATACTTAAA GCTTCCAGAA
CCTTCAGCCT CATTGCCAAA TCCTCCATCA AAGAAAATAA AGTTATCAGA TGAGCCTGTA
GAAGCAAAAG AAGATTACAC TAAGTTTAAT ACTAAAGATT TGAAGACTGA AAAGAAAAAT
AGCAAAATGA CTGCAGCTCA GAAGGCTTTG GCTAAAGTTG ACAAGAGTGG AATGAAAAGT
ATTGATACCT TTTTTGGGGT AAAAAATAAA AAAAAAATTG GAAAGGTTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011533535.1
CDS683..1213
Misc Feature(1)758..1177(+)
Translation
Position Chain Variation Link
5 5 c, t dbSNP:549441721
45 45 c, t dbSNP:562869389
61 61 a, g dbSNP:765552524
76 76 a, g dbSNP:531904107
89 89 a, g dbSNP:551573692
108 108 c, t dbSNP:571566229
115 115 c, t dbSNP:763103642
120 120 a, g dbSNP:764223334
121 121 a, t dbSNP:758877268
138 138 a, t dbSNP:778329909
141 141 a, g dbSNP:748052389
148 148 a, g dbSNP:763308917
152 152 c, g dbSNP:771472509
154 154 -, aag dbSNP:776706264
158 158 a, c dbSNP:772952262
161 161 c, t dbSNP:542600186
164 164 a, g dbSNP:377417708
165 165 a, g dbSNP:775880092
167 167 a, c dbSNP:763229293
171 171 a, g dbSNP:764270783
175 175 a, g dbSNP:774427890
196 196 c, t dbSNP:762281569
197 197 a, c dbSNP:79564863
198 198 a, c dbSNP:199839728
201 201 a, t dbSNP:74876702
203 203 c, t dbSNP:575985863
205 205 -, g dbSNP:762857414
222 222 c, t dbSNP:772503413
225 225 a, g dbSNP:35416748
230 230 a, g dbSNP:770080674
233 233 c, t dbSNP:374465686
239 239 c, t dbSNP:537203779
241 241 c, t dbSNP:75326546
242 242 a, g dbSNP:759251667
246 246 a, g dbSNP:141881672
248 248 g, t dbSNP:75325951
249 249 c, g dbSNP:556877869
252 252 c, t dbSNP:368589166
258 258 a, g dbSNP:35595258
262 262 a, g dbSNP:541903338
262 262 -, g dbSNP:758911416
271 271 c, g dbSNP:145465454
272 272 a, g dbSNP:148811739
287 287 g, t dbSNP:78071087
303 303 a, g dbSNP:757200430
309 309 a, g dbSNP:781187392
311 311 -, aggt dbSNP:780704541
316 316 a, g dbSNP:76158094
319 319 c, g dbSNP:143523027
320 320 g, t dbSNP:747061909
326 326 a, g dbSNP:77931005
332 332 c, g dbSNP:777149786
336 336 -, c dbSNP:781518432
337 337 c, t dbSNP:746338829
338 338 c, g, t dbSNP:770203126
342 342 g, t dbSNP:374098961
346 346 c, g dbSNP:763646959
357 357 c, g dbSNP:774041308
373 373 a, g dbSNP:761380890
377 377 a, g dbSNP:767086382
383 383 a, c dbSNP:201078944
386 386 a, c dbSNP:755936337
393 393 a, g dbSNP:760734846
417 417 c, t dbSNP:766196712
421 421 c, g dbSNP:753716315
425 425 a, g dbSNP:786205483
429 429 c, t dbSNP:759273327
430 430 a, g dbSNP:764980431
434 434 g, t dbSNP:751788888
439 439 a, g dbSNP:757415855
444 444 c, t dbSNP:781310346
445 445 a, g dbSNP:569343532
456 456 a, g dbSNP:756682554
463 463 c, g dbSNP:780398368
464 464 c, g dbSNP:749750512
472 472 a, g dbSNP:769173136
476 476 a, c dbSNP:199935006
481 481 c, t dbSNP:78705382
497 497 a, t dbSNP:200535256
498 498 c, g dbSNP:754434417
510 510 a, g dbSNP:562458672
517 517 a, g dbSNP:75206814
549 549 c, g dbSNP:558373695
592 592 g, t dbSNP:116264598
714 714 a, c dbSNP:746633003
719 719 c, g dbSNP:770355902
729 729 a, g dbSNP:146451037
730 730 c, t dbSNP:759529625
732 732 a, g dbSNP:769721789
739 739 c, t dbSNP:202232001
742 742 c, t dbSNP:775300215
750 750 g, t dbSNP:76219783
757 757 a, g dbSNP:762711560
759 759 a, c dbSNP:75971463
762 762 c, t dbSNP:79310911
772 772 g, t dbSNP:763896138
777 777 a, g dbSNP:78315883
780 780 a, c dbSNP:760884054
789 789 a, g dbSNP:775424614
796 796 c, t dbSNP:200390483
797 797 a, g dbSNP:141049110
802 802 a, g dbSNP:768340427
803 803 a, g dbSNP:75184679
821 821 a, g dbSNP:77377571
823 823 a, g dbSNP:760862553
826 826 c, t dbSNP:766618424
828 828 g, t dbSNP:74555752
829 829 c, g dbSNP:566790226
830 830 a, g dbSNP:759712178
836 836 c, t dbSNP:765368182
837 837 a, g dbSNP:138649673
838 838 c, g dbSNP:141367253
841 841 -, acagtca dbSNP:765643940
841 841 a, g dbSNP:764428875
845 845 a, t dbSNP:555463211
846 846 c, g dbSNP:756903057
850 850 g, t dbSNP:780872918
863 863 g, t dbSNP:745471919
886 886 a, g dbSNP:755648468
889 889 a, g dbSNP:779468408
890 890 a, g dbSNP:17857128
891 891 a, c dbSNP:371740577
892 892 g, t dbSNP:762258182
893 893 a, g dbSNP:376240726
896 896 g, t dbSNP:750077795
902 902 c, t dbSNP:769632378
903 903 a, g dbSNP:755705496
906 906 a, t dbSNP:779596970
910 910 c, t dbSNP:753339416
912 912 a, g dbSNP:200648780
919 919 a, g dbSNP:376122649
923 923 g, t dbSNP:778933609
929 929 c, t dbSNP:77391331
933 933 a, c, t dbSNP:747995413
935 935 c, g dbSNP:777655486
938 938 a, g dbSNP:746153565
951 951 a, g dbSNP:770138442
953 953 a, g dbSNP:775757158
957 957 -, a dbSNP:748406884
957 957 a, t dbSNP:763070137
959 959 c, t dbSNP:768565639
971 971 a, c dbSNP:774950202
973 973 a, g dbSNP:768783637
979 979 a, g dbSNP:774234566
981 981 a, g dbSNP:748746326
993 993 c, g, t dbSNP:372632599
994 994 a, g dbSNP:761005167
1008 1008 c, t dbSNP:190327832
1018 1018 a, c dbSNP:748144224
1030 1030 a, g dbSNP:200721645
1036 1036 a, g dbSNP:146378238
1037 1037 c, g dbSNP:747450548
1040 1040 g, t dbSNP:771299667
1060 1060 c, t dbSNP:776847998
1061 1061 a, g dbSNP:150363383
1062 1062 c, g dbSNP:183734174
1082 1082 c, g, t dbSNP:775119865
1092 1092 a, g dbSNP:763654606
1098 1098 a, g dbSNP:761906095
1099 1099 a, g dbSNP:559000645
1104 1104 a, g dbSNP:200802557
1108 1108 a, g dbSNP:750279427
1117 1117 a, c dbSNP:760409807
1122 1122 a, g dbSNP:766157957
1127 1127 a, g dbSNP:752930177
1128 1128 c, t dbSNP:758605855
1133 1133 g, t dbSNP:144408326
1142 1142 a, g dbSNP:201190805
1162 1162 c, t dbSNP:757835049
1163 1163 a, g dbSNP:781574666
1169 1169 a, g dbSNP:370507842
1173 1173 a, t dbSNP:770055331
1176 1176 c, t dbSNP:780504013
1178 1178 a, g dbSNP:749003546
1181 1181 -, t dbSNP:768221761
1188 1188 a, c dbSNP:768415436
1189 1189 -, a, aa dbSNP:200320729
1189 1189 a, t dbSNP:112937854
1190 1190 -, a dbSNP:761386077
1190 1190 a, t dbSNP:774043353
1199 1199 -, a dbSNP:75254367
1199 1199 a, g dbSNP:761204571
1202 1202 -, ttat dbSNP:772980492
1202 1202 a, g dbSNP:771560732
1225 1225 c, t dbSNP:773052815
1246 1246 g, t dbSNP:529254634
1253 1253 a, t dbSNP:766145608
1262 1262 a, g dbSNP:753627853
1266 1266 c, t dbSNP:549023975
1267 1267 g, t dbSNP:569240707
1276 1276 c, g, t dbSNP:751735556
1280 1280 a, g dbSNP:781270130
1289 1289 a, t dbSNP:750903767
1299 1299 g, t dbSNP:756627118
1315 1315 -, c dbSNP:763452841
1323 1323 a, g dbSNP:761886682
1359 1359 c, t dbSNP:780358371
1459 1459 a, g dbSNP:147825612
1464 1464 g, t dbSNP:747397214
1486 1486 c, t dbSNP:551658315

Target ORF information:

RefSeq Version XM_011535233
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535233

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGGCAGTT TTCATTTCCC ATCAAAAGGT AATCCAGAAA TAGACAACAA GAAATATTAC 
AAGTACAGCA AAGAGAAGAC ATTAAAGTGG CTGGAAAAAA AGGTTAATCA AACTGTGGCA
GCATTAAAAA CCAATAATGT GAATGTCAGT TCCCGGGTAC AGTCAACTGC ATTTTTCTCT
GGTGACCAAG CTTCCACTGA CAAGGAAGAG GATTATATTC GTTATGCCCA TGGTCTGATA
TCTGACTACA TCCCTAAAGA ATTAAGTGAT GACTTATCTA AATACTTAAA GCTTCCAGAA
CCTTCAGCCT CATTGCCAAA TCCTCCATCA AAGAAAATAA AGTTATCAGA TGAGCCTGTA
GAAGCAAAAG AAGATTACAC TAAGTTTAAT ACTAAAGATT TGAAGACTGA AAAGAAAAAT
AGCAAAATGA CTGCAGCTCA GAAGGCTTTG GCTAAAGTTG ACAAGAGTGG AATGAAAAGT
ATTGATACCT TTTTTGGGGT AAAAAATAAA AAAAAAATTG GAAAGGTTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71945
Accession Version XM_011535234.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 516bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ribonuclease H2 subunit B isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_024524.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAA GGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011533536.1
CDS322..837
Misc Feature(1)370..>831(+)
Misc Feature(2)376..546(+)
Translation
Position Chain Variation Link
30 30 c, g dbSNP:544448663
48 48 c, g dbSNP:540984248
94 94 a, c dbSNP:577725827
95 95 a, g dbSNP:564346046
112 112 c, g dbSNP:112702177
114 114 a, g dbSNP:11544097
205 205 c, g dbSNP:553446261
210 210 c, t dbSNP:184573722
246 246 c, g dbSNP:529317356
257 257 c, t dbSNP:549441721
297 297 c, t dbSNP:562869389
313 313 a, g dbSNP:765552524
328 328 a, g dbSNP:531904107
341 341 a, g dbSNP:551573692
360 360 c, t dbSNP:571566229
367 367 c, t dbSNP:763103642
372 372 a, g dbSNP:764223334
373 373 a, t dbSNP:758877268
390 390 a, t dbSNP:778329909
393 393 a, g dbSNP:748052389
400 400 a, g dbSNP:763308917
404 404 c, g dbSNP:771472509
406 406 -, aag dbSNP:776706264
410 410 a, c dbSNP:772952262
413 413 c, t dbSNP:542600186
416 416 a, g dbSNP:377417708
417 417 a, g dbSNP:775880092
419 419 a, c dbSNP:763229293
423 423 a, g dbSNP:764270783
427 427 a, g dbSNP:774427890
448 448 c, t dbSNP:762281569
449 449 a, c dbSNP:79564863
450 450 a, c dbSNP:199839728
453 453 a, t dbSNP:74876702
455 455 c, t dbSNP:575985863
457 457 -, g dbSNP:762857414
474 474 c, t dbSNP:772503413
477 477 a, g dbSNP:35416748
482 482 a, g dbSNP:770080674
485 485 c, t dbSNP:374465686
491 491 c, t dbSNP:537203779
493 493 c, t dbSNP:75326546
494 494 a, g dbSNP:759251667
498 498 a, g dbSNP:141881672
500 500 g, t dbSNP:75325951
501 501 c, g dbSNP:556877869
504 504 c, t dbSNP:368589166
510 510 a, g dbSNP:35595258
514 514 a, g dbSNP:541903338
514 514 -, g dbSNP:758911416
523 523 c, g dbSNP:145465454
524 524 a, g dbSNP:148811739
539 539 g, t dbSNP:78071087
555 555 a, g dbSNP:757200430
561 561 a, g dbSNP:781187392
563 563 -, aggt dbSNP:780704541
568 568 a, g dbSNP:76158094
571 571 c, g dbSNP:143523027
572 572 g, t dbSNP:747061909
578 578 a, g dbSNP:77931005
584 584 c, g dbSNP:777149786
588 588 -, c dbSNP:781518432
589 589 c, t dbSNP:746338829
590 590 c, g, t dbSNP:770203126
594 594 g, t dbSNP:374098961
598 598 c, g dbSNP:763646959
609 609 c, g dbSNP:774041308
625 625 a, g dbSNP:761380890
629 629 a, g dbSNP:767086382
635 635 a, c dbSNP:201078944
638 638 a, c dbSNP:755936337
645 645 a, g dbSNP:760734846
669 669 c, t dbSNP:766196712
673 673 c, g dbSNP:753716315
677 677 a, g dbSNP:786205483
681 681 c, t dbSNP:759273327
682 682 a, g dbSNP:764980431
686 686 g, t dbSNP:751788888
691 691 a, g dbSNP:757415855
696 696 c, t dbSNP:781310346
697 697 a, g dbSNP:569343532
708 708 a, g dbSNP:756682554
715 715 c, g dbSNP:780398368
716 716 c, g dbSNP:749750512
724 724 a, g dbSNP:769173136
728 728 a, c dbSNP:199935006
733 733 c, t dbSNP:78705382
749 749 a, t dbSNP:200535256
750 750 c, g dbSNP:754434417
761 761 a, c dbSNP:746633003
766 766 c, g dbSNP:770355902
776 776 a, g dbSNP:146451037
777 777 c, t dbSNP:759529625
779 779 a, g dbSNP:769721789
786 786 c, t dbSNP:202232001
789 789 c, t dbSNP:775300215
797 797 g, t dbSNP:76219783
804 804 a, g dbSNP:762711560
806 806 a, c dbSNP:75971463
809 809 c, t dbSNP:79310911
819 819 g, t dbSNP:763896138
824 824 a, g dbSNP:78315883
827 827 a, c dbSNP:760884054
837 837 a, g dbSNP:775424614
844 844 c, t dbSNP:200390483
845 845 a, g dbSNP:141049110
850 850 a, g dbSNP:768340427
851 851 a, g dbSNP:75184679
869 869 a, g dbSNP:77377571
871 871 a, g dbSNP:760862553
874 874 c, t dbSNP:766618424
876 876 g, t dbSNP:74555752
877 877 c, g dbSNP:566790226
878 878 a, g dbSNP:759712178
884 884 c, t dbSNP:765368182
885 885 a, g dbSNP:138649673
886 886 c, g dbSNP:141367253
889 889 -, acagtca dbSNP:765643940
889 889 a, g dbSNP:764428875
893 893 a, t dbSNP:555463211
894 894 c, g dbSNP:756903057
898 898 g, t dbSNP:780872918
911 911 g, t dbSNP:745471919

Target ORF information:

RefSeq Version XM_011535234
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ribonuclease H2, subunit B (RNASEH2B), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535234

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAA GGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23058
Accession Version NM_001142279.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 774bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ribonuclease H2 subunit B isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL137881.12 and CA309004.1. This sequence is a reference standard in the RefSeqGene project. On Mar 26, 2011 this sequence version replaced gi:214010190. Summary: RNase H2 is composed of a single catalytic subunit (A) and two non-catalytic subunits (B and C) and specifically degrades the RNA of RNA:DNA hybrids. The protein encoded by this gene is the non-catalytic B subunit of RNase H2, which is thought to play a role in DNA replication. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Aicardi-Goutieres syndrome type 2 (AGS2). [provided by RefSeq, Nov 2008]. Transcript Variant: This variant (2) uses an alternate splice pattern in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCCGCTG GCGTGGACTG CGGGGACGGG GTTGGCGCCC GGCAGCACGT GTTCCTGGTT 
TCAGAATATT TAAAAGATGC TTCAAAGAAG ATGAAAAATG GGCTAATGTT TGTAAAACTG
GTTAACCCCT GTTCAGGAGA AGGAGCCATT TACTTGTTCA ATATGTGTCT ACAGCAGCTG
TTTGAAGTAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA
TCAGGAGGTC TTCTCCATTT TGCCACACCT GTGGATCCTC TATTTCTGCT TCTCCACTAC
CTCATAAAGG CTGATAAGGA GGGGAAGTTT CAGCCCCTTG ATCAAGTTGT GGTGGATAAC
GTGTTTCCAA ATTGCATCTT GTTGCTGAAA CTTCCTGGAC TTGAGAAGTT ACTTCATCAT
GTGACAGAGG AAAAAGGTAA TCCAGAAATA GACAACAAGA AATATTACAA GTACAGCAAA
GAGAAGACAT TAAAGTGGCT GGAAAAAAAG GTTAATCAAA CTGTGGCAGC ATTAAAAACC
AATAATGTGA ATGTCAGTTC CCGGGTACAG TCAACTGCAT TTTTCTCTGG TGACCAAGCT
TCCACTGACA AGGAAGAGGA TTATATTCGT TATGCCCATG GTCTGATATC TGACTACATC
CCTAAAGAAT TAAGTGATGA CTTATCTAAA TACTTAAAGC TTCCAGAACC TTCAGCCTCA
TTGCCAAATC CTCCATCAAA GATGGCAGCA CAAAGACAGA AAAGGGGCAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001135751.1
CDS400..1173
Misc Feature(1)367..369(+)
Misc Feature(2)403..405(+)
Misc Feature(3)448..>960(+)
Misc Feature(4)454..624(+)
Exon (1)1..463
Gene:RNASEH2B
Gene Synonym:
Exon (2)464..535
Gene:RNASEH2B
Gene Synonym:
Exon (3)536..643
Gene:RNASEH2B
Gene Synonym:
Exon (4)644..720
Gene:RNASEH2B
Gene Synonym:
Exon (5)721..835
Gene:RNASEH2B
Gene Synonym:
Exon (6)836..909
Gene:RNASEH2B
Gene Synonym:
Exon (7)910..1015
Gene:RNASEH2B
Gene Synonym:
Exon (8)1016..1097
Gene:RNASEH2B
Gene Synonym:
Exon (9)1098..1140
Gene:RNASEH2B
Gene Synonym:
Exon (10)1141..1669
Gene:RNASEH2B
Gene Synonym:
Translation
Position Chain Variation Link
1 1 c, g dbSNP:553965425
108 108 c, g dbSNP:544448663
126 126 c, g dbSNP:540984248
172 172 a, c dbSNP:577725827
173 173 a, g dbSNP:564346046
190 190 c, g dbSNP:112702177
192 192 a, g dbSNP:11544097
283 283 c, g dbSNP:553446261
288 288 c, t dbSNP:184573722
324 324 c, g dbSNP:529317356
335 335 c, t dbSNP:549441721
375 375 c, t dbSNP:562869389
391 391 a, g dbSNP:765552524
406 406 a, g dbSNP:531904107
419 419 a, g dbSNP:551573692
438 438 c, t dbSNP:571566229
445 445 c, t dbSNP:763103642
450 450 a, g dbSNP:764223334
451 451 a, t dbSNP:758877268
468 468 a, t dbSNP:778329909
471 471 a, g dbSNP:748052389
478 478 a, g dbSNP:763308917
482 482 c, g dbSNP:771472509
484 484 -, aag dbSNP:776706264
488 488 a, c dbSNP:772952262
491 491 c, t dbSNP:542600186
494 494 a, g dbSNP:377417708
495 495 a, g dbSNP:775880092
497 497 a, c dbSNP:763229293
501 501 a, g dbSNP:764270783
505 505 a, g dbSNP:774427890
526 526 c, t dbSNP:762281569
527 527 a, c dbSNP:79564863
528 528 a, c dbSNP:199839728
531 531 a, t dbSNP:74876702
533 533 c, t dbSNP:575985863
535 535 -, g dbSNP:762857414
552 552 c, t dbSNP:772503413
555 555 a, g dbSNP:35416748
560 560 a, g dbSNP:770080674
563 563 c, t dbSNP:374465686
569 569 c, t dbSNP:537203779
571 571 c, t dbSNP:75326546
572 572 a, g dbSNP:759251667
576 576 a, g dbSNP:141881672
578 578 g, t dbSNP:75325951
579 579 c, g dbSNP:556877869
582 582 c, t dbSNP:368589166
588 588 a, g dbSNP:35595258
592 592 a, g dbSNP:541903338
592 592 -, g dbSNP:758911416
601 601 c, g dbSNP:145465454
602 602 a, g dbSNP:148811739
617 617 g, t dbSNP:78071087
633 633 a, g dbSNP:757200430
639 639 a, g dbSNP:781187392
641 641 -, aggt dbSNP:780704541
646 646 a, g dbSNP:76158094
649 649 c, g dbSNP:143523027
650 650 g, t dbSNP:747061909
656 656 a, g dbSNP:77931005
662 662 c, g dbSNP:777149786
666 666 -, c dbSNP:781518432
667 667 c, t dbSNP:746338829
668 668 c, g, t dbSNP:770203126
672 672 g, t dbSNP:374098961
676 676 c, g dbSNP:763646959
687 687 c, g dbSNP:774041308
703 703 a, g dbSNP:761380890
707 707 a, g dbSNP:767086382
713 713 a, c dbSNP:201078944
716 716 a, c dbSNP:755936337
723 723 a, g dbSNP:760734846
747 747 c, t dbSNP:766196712
751 751 c, g