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USB1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol USB1
Entrez Gene ID 79650
Full Name U6 snRNA biogenesis 1
Synonyms C16orf57, HVSL1, Mpn1, PN, hUsb1
General protein information
Preferred Names
U6 snRNA phosphodiesterase
Names
U6 snRNA phosphodiesterase
U six biogenesis 1
HVSL motif containing 1
UPF0406 protein C16orf57
putative U6 snRNA phosphodiesterase
mutated in poikiloderma with neutropenia protein 1
NP_001182231.1
NP_001191840.1
NP_078874.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16q21

Summary This gene encodes a protein with several conserved domains, however, its exact function is not known. Mutations in this gene are associated with poikiloderma with neutropenia (PN), which shows phenotypic overlap with Rothmund-Thomson syndrome (RTS) caused by mutations in the RECQL4 gene. It is believed that this gene product interacts with RECQL4 protein via SMAD4 proteins, explaining the partial clinical overlap between PN and RTS. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]. lac of sum
Disorder MIM:

613276

Disorder Html: Poikiloderma with neutropenia, 604173 (3)





The following USB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu71969 XM_011523328 PREDICTED: Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
OHu49499 XM_005256144 PREDICTED: Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199.00
OHu49499 XM_011523329 PREDICTED: Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199.00
OHu71970 XM_011523330 PREDICTED: Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99.00
OHu08584 NM_001204911 Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99.00
OHu20716 NM_024598 Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00
OHu07663 NM_001195302 Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu71969
Accession Version XM_011523328.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 759bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product U6 snRNA phosphodiesterase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGAGTCCGA GGACGGGATG CGGACCAGGC CGGGGGATGG GAGCCACCGT CGGTGAGGAT 
GGCCAGAGCC CCCTTCCCAG GCAGAGATTT CCAGTACCTG ACAGTGTGCT GAACATGTTC
CCGGGCACCG AGGAGGGGCC TGAAGATGAC AGCACAAAAC ACGGGGGACG GGTGCGCACC
TTCCCCCACG AGCGAGGCAA CTGGGCCACC CACGTCTATG TACCATATGA AGCCAAGGAG
GAGTTCCTGG ATCTGCTTGA TGTGTTGCTG CCCCATGCCC AGACATATGT CCCCCGGCTG
GTAAGGATGA AGGTGTTCCA CCTCAGCCTG TCCCAGAGTG TGGTTCTGCG CCACCACTGG
ATCCTCCCCT TCGTGCAGGC TCTGAAAGCC CGTATGACCT CCTTCCACAG ATTCTTCTTT
ACTGCCAACC AGGTAAAGAT TTACACCAAT CAAGAGAAAA CCAGGACCTT TATTGGGCTT
GAGGTCACTT CAGGGCATGC CCAGTTCCTG GACCTGGTTT CAGAGGTGGA CAGAGTCATG
GAGGAATTCA ACCTCACCAC TTTCTACCAG GATCCTTCTT TCCACCTCAG CCTGGCCTGG
TGTGTGGGTG ATGCACGTCT CCAGCTGGAG GGGCAGTGCC TGCAGGAACT ACAGGCAATC
GTGGATGGGT TTGAAGATGC TGAGGTGCTG CTGCGCGTGC ACACTGAGCA AGTCCGCTGC
AAGTCTGGGA ACAAGTTCTT CTCGATGCCT TTGAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011521630.1
CDS124..882
Misc Feature(1)214..876(+)
Translation
Position Chain Variation Link
7 7 a, g dbSNP:749839080
8 8 a, g dbSNP:557453429
15 15 g, t dbSNP:572460232
21 21 c, t dbSNP:539851929
25 25 g, t dbSNP:555381360
27 27 c, g dbSNP:73546949
29 29 c, t dbSNP:755207534
30 30 a, g dbSNP:544521629
32 32 g, t dbSNP:748260509
41 41 c, g dbSNP:758458788
44 44 a, g dbSNP:562526527
48 48 a, g dbSNP:778195515
49 49 a, c dbSNP:747515828
52 52 a, c, g dbSNP:201250414
58 58 c, g dbSNP:746174189
65 65 a, t dbSNP:768425672
66 66 g, t dbSNP:774106572
67 67 a, c, g dbSNP:543492919
70 70 a, g dbSNP:370555892
73 73 g, t dbSNP:773091216
78 78 a, g dbSNP:760648279
80 80 c, g dbSNP:546288690
83 83 c, t dbSNP:753636337
90 90 -, c dbSNP:781151070
90 90 c, t dbSNP:759290159
92 92 c, t dbSNP:765469310
97 97 c, t dbSNP:373657742
100 100 a, g dbSNP:564572047
107 107 c, g dbSNP:777899627
108 108 a, t dbSNP:752060249
115 115 a, g dbSNP:757792250
119 119 a, c dbSNP:3743559
131 131 c, g dbSNP:746264362
133 133 a, g dbSNP:770232543
138 138 a, g dbSNP:778756392
138 138 -, g dbSNP:747958554
144 144 c, t dbSNP:747805446
145 145 c, g dbSNP:771539759
151 151 c, g dbSNP:772792606
155 155 c, g dbSNP:375706004
157 157 c, g dbSNP:770934785
167 167 a, c dbSNP:149411375
174 174 c, g dbSNP:759378341
178 178 a, g dbSNP:764990301
184 184 a, g dbSNP:769630452
186 186 c, g, t dbSNP:144716560
188 188 a, c dbSNP:762585706
190 190 a, g dbSNP:138405924
192 192 a, c dbSNP:774385750
193 193 c, t dbSNP:762001177
195 195 c, t dbSNP:767672950
199 199 c, t dbSNP:751037751
208 208 a, t dbSNP:756702350
213 213 c, t dbSNP:766926835
218 218 -, t dbSNP:774728454
232 232 c, t dbSNP:754262096
233 233 c, t dbSNP:755250981
246 246 a, g dbSNP:143792185
248 248 a, g dbSNP:746613694
249 249 c, g, t dbSNP:756831751
253 253 a, g dbSNP:745321157
254 254 a, c dbSNP:769724028
258 258 -, gg dbSNP:759761915
263 263 -, c dbSNP:786205051
272 272 a, t dbSNP:775249170
278 278 a, c dbSNP:749129393
285 285 c, t dbSNP:548754947
286 286 a, g dbSNP:774672635
289 289 a, g dbSNP:762121009
290 290 a, g dbSNP:767791131
292 292 a, c, t dbSNP:531886363
293 293 a, g dbSNP:767016894
297 297 a, g dbSNP:369515724
298 298 c, t dbSNP:567360825
299 299 a, c, g dbSNP:372917091
302 302 c, t dbSNP:751288061
308 308 c, t dbSNP:763524876
310 310 a, c dbSNP:780752842
312 312 c, t dbSNP:549783755
314 314 a, c dbSNP:755570589
315 315 g, t dbSNP:371393773
316 316 a, c dbSNP:374559185
327 327 a, g dbSNP:137853973
328 328 a, g dbSNP:778559247
336 336 c, t dbSNP:147246452
337 337 a, g dbSNP:764580227
339 339 c, t dbSNP:375733174
342 342 c, t dbSNP:199820065
343 343 a, g dbSNP:571564926
347 347 c, g dbSNP:770881616
351 351 c, t dbSNP:771096742
360 360 a, g dbSNP:776583555
374 374 a, t dbSNP:759598117
387 387 a, g dbSNP:770239330
396 396 c, t dbSNP:140760824
401 401 c, t dbSNP:763359090
409 409 -, t dbSNP:754368529
410 410 a, g dbSNP:764413085
411 411 c, t dbSNP:369321909
419 419 a, g dbSNP:142683238
420 420 a, g, t dbSNP:765998448
428 428 a, g dbSNP:35025252
434 434 a, g dbSNP:778763495
442 442 c, t dbSNP:752648429
447 447 c, t dbSNP:758332726
451 451 c, g, t dbSNP:777694507
461 461 a, g dbSNP:757421099
465 465 a, g dbSNP:781440519
471 471 c, g dbSNP:542658449
472 472 c, t dbSNP:769898628
473 473 a, g dbSNP:199886884
486 486 c, g dbSNP:776019365
495 495 c, t dbSNP:749793676
496 496 a, g dbSNP:769067404
507 507 c, g dbSNP:774712588
509 509 a, g dbSNP:762197073
514 514 c, t dbSNP:774644058
515 515 a, g dbSNP:367780234
515 515 -, g dbSNP:762227910
531 531 c, g dbSNP:776322680
535 535 -, ttc dbSNP:755973489
536 536 a, t dbSNP:375204675
545 545 c, t dbSNP:779326318
550 550 a, c dbSNP:748682814
552 552 c, t dbSNP:372875085
554 554 a, g dbSNP:202096477
567 567 c, t dbSNP:747267362
570 570 -, caat dbSNP:777667891
573 573 c, t dbSNP:769596860
582 582 a, c dbSNP:775237878
583 583 a, g dbSNP:139558948
584 584 c, t dbSNP:768253665
585 585 c, t dbSNP:774223335
586 586 a, g dbSNP:137853971
589 589 a, t dbSNP:779896567
596 596 c, t dbSNP:149725439
604 604 c, g dbSNP:768341871
617 617 a, g dbSNP:374426055
619 619 c, t dbSNP:762828282
624 624 c, g dbSNP:761161720
648 648 a, g dbSNP:772079518
652 652 a, g dbSNP:772999682
655 655 a, g dbSNP:760502631
667 667 a, g dbSNP:766156595
668 668 a, c dbSNP:754116399
674 674 a, g dbSNP:377411772
676 676 c, t dbSNP:765307761
677 677 g, t dbSNP:555309420
682 682 a, g dbSNP:758357702
683 683 c, g dbSNP:764715431
690 690 c, t dbSNP:764176381
708 708 c, t dbSNP:371949436
723 723 a, c, g dbSNP:750851175
725 725 a, g dbSNP:146685901
730 730 a, g dbSNP:778666156
739 739 c, t dbSNP:554516072
740 740 a, g dbSNP:757982322
751 751 c, g dbSNP:777276672
755 755 a, g dbSNP:746447180
777 777 a, g dbSNP:770883144
781 781 a, g dbSNP:769032208
783 783 c, t dbSNP:774669193
784 784 a, g dbSNP:762033139
796 796 a, g dbSNP:772272501
800 800 a, g dbSNP:773826400
807 807 c, g dbSNP:761098113
809 809 c, t dbSNP:766841291
813 813 a, g dbSNP:190283472
817 817 c, t dbSNP:182418203
818 818 a, g dbSNP:140289507
819 819 c, t dbSNP:763780991
820 820 a, g dbSNP:751177454
829 829 a, g dbSNP:756831672
832 832 c, g dbSNP:16959641
838 838 a, c, t dbSNP:146419360
839 839 a, g dbSNP:780003077
855 855 c, g dbSNP:149041481
866 866 c, t dbSNP:368558020
867 867 c, g dbSNP:779320272
868 868 a, g dbSNP:143382202
871 871 c, t dbSNP:772360295
872 872 c, t dbSNP:773169264
879 879 a, g dbSNP:760767630
884 884 c, t dbSNP:771359873
886 886 a, c dbSNP:777169639
896 896 c, t dbSNP:759932035
906 906 c, g dbSNP:765579501
922 922 a, g dbSNP:774051099
984 984 a, c dbSNP:772110530
1030 1030 c, g dbSNP:772987427
1048 1048 -, tctc dbSNP:370473790
1060 1060 -, tt dbSNP:759921215
1076 1076 -, ttct dbSNP:372237467
1087 1087 -, ctc dbSNP:752765084
1090 1090 -, ctct dbSNP:200950416
1092 1092 -, ct dbSNP:762941380
1103 1103 -, ct dbSNP:751796506
1104 1104 -, tctg dbSNP:142769438
1107 1107 -, gtct dbSNP:375560719
1108 1108 g, t dbSNP:35071272
1121 1121 -, ct dbSNP:549920112
1133 1133 c, t dbSNP:575397821
1135 1135 c, t dbSNP:113112209
1136 1136 -, tctc dbSNP:547553387
1143 1143 -, tcctc dbSNP:527401961
1166 1166 -, tctc dbSNP:547883874
1178 1178 c, g, t dbSNP:113876388
1183 1183 -, tg dbSNP:754995890
1184 1184 c, g dbSNP:549064355
1186 1186 c, t dbSNP:565377163
1188 1188 -, ctcctcccctc dbSNP:747135009
1198 1198 -, ct, ctct dbSNP:112874007
1198 1198 -, c dbSNP:369788319
1199 1199 -, ctct dbSNP:72112945
1201 1201 -, ctcttcctctc dbSNP:749283271
1201 1201 -, ctct dbSNP:386384807
1203 1203 -, cctc dbSNP:751375096
1204 1204 -, ctct dbSNP:58782258
1205 1205 c, t dbSNP:151105116
1206 1206 c, t dbSNP:202140498
1211 1211 c, g dbSNP:532682469
1212 1212 -, ct dbSNP:553004572
1224 1224 -, ctc dbSNP:563553918
1227 1227 -, ct dbSNP:747734953
1235 1235 a, t dbSNP:113866163
1258 1258 -, ct dbSNP:139623690
1274 1274 -, ctct dbSNP:565062214
1279 1279 -, tctc dbSNP:201974024
1291 1291 -, ct dbSNP:371978648
1310 1310 c, t dbSNP:547797939
1315 1315 c, t dbSNP:370068353
1320 1320 c, g dbSNP:536035144
1353 1353 c, t dbSNP:559803513
1354 1354 c, g dbSNP:530392108
1374 1374 -, ctct dbSNP:150380411
1376 1376 c, g dbSNP:373066608
1378 1378 a, c dbSNP:548868928
1404 1404 c, g dbSNP:12930821
1414 1414 c, g dbSNP:531016950
1421 1421 c, t dbSNP:552540257
1422 1422 c, t dbSNP:570503152
1442 1442 a, g dbSNP:191811468
1446 1446 a, g dbSNP:183630288
1458 1458 c, t dbSNP:376388970
1460 1460 c, t dbSNP:574116680
1528 1528 c, g dbSNP:185385414
1539 1539 c, t dbSNP:539869982
1546 1546 c, g dbSNP:575497717
1553 1553 a, g dbSNP:545958819
1554 1554 c, t dbSNP:564161180
1555 1555 a, g dbSNP:577377913
1609 1609 c, t dbSNP:768960345
1611 1611 a, c dbSNP:774311786
1621 1621 c, t dbSNP:11551263
1625 1625 c, t dbSNP:765434178
1703 1703 c, g dbSNP:753128270
1727 1727 g, t dbSNP:190241788
1729 1729 a, g dbSNP:182095431
1731 1731 g, t dbSNP:758660280
1766 1766 c, t dbSNP:144654181
1812 1812 c, t dbSNP:62041779
1834 1834 a, g dbSNP:540042603
1836 1836 c, g dbSNP:755679865
1854 1854 a, g dbSNP:552719952
1862 1862 c, t dbSNP:779638358
1867 1867 a, g dbSNP:748960478
1881 1881 c, t dbSNP:148180082
1889 1889 c, g dbSNP:375005880
1914 1914 a, g dbSNP:113980652
2007 2007 a, t dbSNP:778701096
2027 2027 a, g dbSNP:748012386
2081 2081 a, g, t dbSNP:528146538
2124 2124 c, t dbSNP:186728160
2136 2136 a, g dbSNP:773236980
2163 2163 a, g dbSNP:567676558
2200 2200 c, t dbSNP:11551264
2206 2206 -, ac dbSNP:776133409
2207 2207 c, t dbSNP:535429733

Target ORF information:

RefSeq Version XM_011523328
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011523328

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGAGTCCGA GGACGGGATG CGGACCAGGC CGGGGGATGG GAGCCACCGT CGGTGAGGAT 
GGCCAGAGCC CCCTTCCCAG GCAGAGATTT CCAGTACCTG ACAGTGTGCT GAACATGTTC
CCGGGCACCG AGGAGGGGCC TGAAGATGAC AGCACAAAAC ACGGGGGACG GGTGCGCACC
TTCCCCCACG AGCGAGGCAA CTGGGCCACC CACGTCTATG TACCATATGA AGCCAAGGAG
GAGTTCCTGG ATCTGCTTGA TGTGTTGCTG CCCCATGCCC AGACATATGT CCCCCGGCTG
GTAAGGATGA AGGTGTTCCA CCTCAGCCTG TCCCAGAGTG TGGTTCTGCG CCACCACTGG
ATCCTCCCCT TCGTGCAGGC TCTGAAAGCC CGTATGACCT CCTTCCACAG ATTCTTCTTT
ACTGCCAACC AGGTAAAGAT TTACACCAAT CAAGAGAAAA CCAGGACCTT TATTGGGCTT
GAGGTCACTT CAGGGCATGC CCAGTTCCTG GACCTGGTTT CAGAGGTGGA CAGAGTCATG
GAGGAATTCA ACCTCACCAC TTTCTACCAG GATCCTTCTT TCCACCTCAG CCTGGCCTGG
TGTGTGGGTG ATGCACGTCT CCAGCTGGAG GGGCAGTGCC TGCAGGAACT ACAGGCAATC
GTGGATGGGT TTGAAGATGC TGAGGTGCTG CTGCGCGTGC ACACTGAGCA AGTCCGCTGC
AAGTCTGGGA ACAAGTTCTT CTCGATGCCT TTGAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu49499
Accession Version XM_005256144.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 645bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product U6 snRNA phosphodiesterase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578829195. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGTTCCCGG GCACCGAGGA GGGGCCTGAA GATGACAGCA CAAAACACGG GGGACGGGTG 
CGCACCTTCC CCCACGAGCG AGGCAACTGG GCCACCCACG TCTATGTACC ATATGAAGCC
AAGGAGGAGT TCCTGGATCT GCTTGATGTG TTGCTGCCCC ATGCCCAGAC ATATGTCCCC
CGGCTGGTAA GGATGAAGGT GTTCCACCTC AGCCTGTCCC AGAGTGTGGT TCTGCGCCAC
CACTGGATCC TCCCCTTCGT GCAGGCTCTG AAAGCCCGTA TGACCTCCTT CCACAGATTC
TTCTTTACTG CCAACCAGGT AAAGATTTAC ACCAATCAAG AGAAAACCAG GACCTTTATT
GGGCTTGAGG TCACTTCAGG GCATGCCCAG TTCCTGGACC TGGTTTCAGA GGTGGACAGA
GTCATGGAGG AATTCAACCT CACCACTTTC TACCAGGATC CTTCTTTCCA CCTCAGCCTG
GCCTGGTGTG TGGGTGATGC ACGTCTCCAG CTGGAGGGGC AGTGCCTGCA GGAACTACAG
GCAATCGTGG ATGGGTTTGA AGATGCTGAG GTGCTGCTGC GCGTGCACAC TGAGCAAGTC
CGCTGCAAGT CTGGGAACAA GTTCTTCTCG ATGCCTTTGA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005256201.1
CDS140..784
Misc Feature(1)140..778(+)
Translation
Position Chain Variation Link
24 24 a, g dbSNP:779832739
35 35 c, g dbSNP:749150624
41 41 c, t dbSNP:191833236
71 71 c, t dbSNP:768429268
86 86 a, g dbSNP:769630452
88 88 c, g, t dbSNP:144716560
90 90 a, c dbSNP:762585706
92 92 a, g dbSNP:138405924
94 94 a, c dbSNP:774385750
95 95 c, t dbSNP:762001177
97 97 c, t dbSNP:767672950
101 101 c, t dbSNP:751037751
110 110 a, t dbSNP:756702350
115 115 c, t dbSNP:766926835
120 120 -, t dbSNP:774728454
134 134 c, t dbSNP:754262096
135 135 c, t dbSNP:755250981
148 148 a, g dbSNP:143792185
150 150 a, g dbSNP:746613694
151 151 c, g, t dbSNP:756831751
155 155 a, g dbSNP:745321157
156 156 a, c dbSNP:769724028
160 160 -, gg dbSNP:759761915
165 165 -, c dbSNP:786205051
174 174 a, t dbSNP:775249170
180 180 a, c dbSNP:749129393
187 187 c, t dbSNP:548754947
188 188 a, g dbSNP:774672635
191 191 a, g dbSNP:762121009
192 192 a, g dbSNP:767791131
194 194 a, c, t dbSNP:531886363
195 195 a, g dbSNP:767016894
199 199 a, g dbSNP:369515724
200 200 c, t dbSNP:567360825
201 201 a, c, g dbSNP:372917091
204 204 c, t dbSNP:751288061
210 210 c, t dbSNP:763524876
212 212 a, c dbSNP:780752842
214 214 c, t dbSNP:549783755
216 216 a, c dbSNP:755570589
217 217 g, t dbSNP:371393773
218 218 a, c dbSNP:374559185
229 229 a, g dbSNP:137853973
230 230 a, g dbSNP:778559247
238 238 c, t dbSNP:147246452
239 239 a, g dbSNP:764580227
241 241 c, t dbSNP:375733174
244 244 c, t dbSNP:199820065
245 245 a, g dbSNP:571564926
249 249 c, g dbSNP:770881616
253 253 c, t dbSNP:771096742
262 262 a, g dbSNP:776583555
276 276 a, t dbSNP:759598117
289 289 a, g dbSNP:770239330
298 298 c, t dbSNP:140760824
303 303 c, t dbSNP:763359090
311 311 -, t dbSNP:754368529
312 312 a, g dbSNP:764413085
313 313 c, t dbSNP:369321909
321 321 a, g dbSNP:142683238
322 322 a, g, t dbSNP:765998448
330 330 a, g dbSNP:35025252
336 336 a, g dbSNP:778763495
344 344 c, t dbSNP:752648429
349 349 c, t dbSNP:758332726
353 353 c, g, t dbSNP:777694507
363 363 a, g dbSNP:757421099
367 367 a, g dbSNP:781440519
373 373 c, g dbSNP:542658449
374 374 c, t dbSNP:769898628
375 375 a, g dbSNP:199886884
388 388 c, g dbSNP:776019365
397 397 c, t dbSNP:749793676
398 398 a, g dbSNP:769067404
409 409 c, g dbSNP:774712588
411 411 a, g dbSNP:762197073
416 416 c, t dbSNP:774644058
417 417 a, g dbSNP:367780234
417 417 -, g dbSNP:762227910
433 433 c, g dbSNP:776322680
437 437 -, ttc dbSNP:755973489
438 438 a, t dbSNP:375204675
447 447 c, t dbSNP:779326318
452 452 a, c dbSNP:748682814
454 454 c, t dbSNP:372875085
456 456 a, g dbSNP:202096477
469 469 c, t dbSNP:747267362
472 472 -, caat dbSNP:777667891
475 475 c, t dbSNP:769596860
484 484 a, c dbSNP:775237878
485 485 a, g dbSNP:139558948
486 486 c, t dbSNP:768253665
487 487 c, t dbSNP:774223335
488 488 a, g dbSNP:137853971
491 491 a, t dbSNP:779896567
498 498 c, t dbSNP:149725439
506 506 c, g dbSNP:768341871
519 519 a, g dbSNP:374426055
521 521 c, t dbSNP:762828282
526 526 c, g dbSNP:761161720
550 550 a, g dbSNP:772079518
554 554 a, g dbSNP:772999682
557 557 a, g dbSNP:760502631
569 569 a, g dbSNP:766156595
570 570 a, c dbSNP:754116399
576 576 a, g dbSNP:377411772
578 578 c, t dbSNP:765307761
579 579 g, t dbSNP:555309420
584 584 a, g dbSNP:758357702
585 585 c, g dbSNP:764715431
592 592 c, t dbSNP:764176381
610 610 c, t dbSNP:371949436
625 625 a, c, g dbSNP:750851175
627 627 a, g dbSNP:146685901
632 632 a, g dbSNP:778666156
641 641 c, t dbSNP:554516072
642 642 a, g dbSNP:757982322
653 653 c, g dbSNP:777276672
657 657 a, g dbSNP:746447180
679 679 a, g dbSNP:770883144
683 683 a, g dbSNP:769032208
685 685 c, t dbSNP:774669193
686 686 a, g dbSNP:762033139
698 698 a, g dbSNP:772272501
702 702 a, g dbSNP:773826400
709 709 c, g dbSNP:761098113
711 711 c, t dbSNP:766841291
715 715 a, g dbSNP:190283472
719 719 c, t dbSNP:182418203
720 720 a, g dbSNP:140289507
721 721 c, t dbSNP:763780991
722 722 a, g dbSNP:751177454
731 731 a, g dbSNP:756831672
734 734 c, g dbSNP:16959641
740 740 a, c, t dbSNP:146419360
741 741 a, g dbSNP:780003077
757 757 c, g dbSNP:149041481
768 768 c, t dbSNP:368558020
769 769 c, g dbSNP:779320272
770 770 a, g dbSNP:143382202
773 773 c, t dbSNP:772360295
774 774 c, t dbSNP:773169264
781 781 a, g dbSNP:760767630
786 786 c, t dbSNP:771359873
788 788 a, c dbSNP:777169639
798 798 c, t dbSNP:759932035
808 808 c, g dbSNP:765579501
824 824 a, g dbSNP:774051099
886 886 a, c dbSNP:772110530
932 932 c, g dbSNP:772987427
950 950 -, tctc dbSNP:370473790
962 962 -, tt dbSNP:759921215
978 978 -, ttct dbSNP:372237467
989 989 -, ctc dbSNP:752765084
992 992 -, ctct dbSNP:200950416
994 994 -, ct dbSNP:762941380
1005 1005 -, ct dbSNP:751796506
1006 1006 -, tctg dbSNP:142769438
1009 1009 -, gtct dbSNP:375560719
1010 1010 g, t dbSNP:35071272
1023 1023 -, ct dbSNP:549920112
1035 1035 c, t dbSNP:575397821
1037 1037 c, t dbSNP:113112209
1038 1038 -, tctc dbSNP:547553387
1045 1045 -, tcctc dbSNP:527401961
1068 1068 -, tctc dbSNP:547883874
1080 1080 c, g, t dbSNP:113876388
1085 1085 -, tg dbSNP:754995890
1086 1086 c, g dbSNP:549064355
1088 1088 c, t dbSNP:565377163
1090 1090 -, ctcctcccctc dbSNP:747135009
1100 1100 -, ct, ctct dbSNP:112874007
1100 1100 -, c dbSNP:369788319
1101 1101 -, ctct dbSNP:72112945
1103 1103 -, ctcttcctctc dbSNP:749283271
1103 1103 -, ctct dbSNP:386384807
1105 1105 -, cctc dbSNP:751375096
1106 1106 -, ctct dbSNP:58782258
1107 1107 c, t dbSNP:151105116
1108 1108 c, t dbSNP:202140498
1113 1113 c, g dbSNP:532682469
1114 1114 -, ct dbSNP:553004572
1126 1126 -, ctc dbSNP:563553918
1129 1129 -, ct dbSNP:747734953
1137 1137 a, t dbSNP:113866163
1160 1160 -, ct dbSNP:139623690
1176 1176 -, ctct dbSNP:565062214
1181 1181 -, tctc dbSNP:201974024
1193 1193 -, ct dbSNP:371978648
1212 1212 c, t dbSNP:547797939
1217 1217 c, t dbSNP:370068353
1222 1222 c, g dbSNP:536035144
1255 1255 c, t dbSNP:559803513
1256 1256 c, g dbSNP:530392108
1276 1276 -, ctct dbSNP:150380411
1278 1278 c, g dbSNP:373066608
1280 1280 a, c dbSNP:548868928
1306 1306 c, g dbSNP:12930821
1316 1316 c, g dbSNP:531016950
1323 1323 c, t dbSNP:552540257
1324 1324 c, t dbSNP:570503152
1344 1344 a, g dbSNP:191811468
1348 1348 a, g dbSNP:183630288
1360 1360 c, t dbSNP:376388970
1362 1362 c, t dbSNP:574116680
1430 1430 c, g dbSNP:185385414
1441 1441 c, t dbSNP:539869982
1448 1448 c, g dbSNP:575497717
1455 1455 a, g dbSNP:545958819
1456 1456 c, t dbSNP:564161180
1457 1457 a, g dbSNP:577377913
1511 1511 c, t dbSNP:768960345
1513 1513 a, c dbSNP:774311786
1523 1523 c, t dbSNP:11551263
1527 1527 c, t dbSNP:765434178
1605 1605 c, g dbSNP:753128270
1629 1629 g, t dbSNP:190241788
1631 1631 a, g dbSNP:182095431
1633 1633 g, t dbSNP:758660280
1668 1668 c, t dbSNP:144654181
1714 1714 c, t dbSNP:62041779
1736 1736 a, g dbSNP:540042603
1738 1738 c, g dbSNP:755679865
1756 1756 a, g dbSNP:552719952
1764 1764 c, t dbSNP:779638358
1769 1769 a, g dbSNP:748960478
1783 1783 c, t dbSNP:148180082
1791 1791 c, g dbSNP:375005880
1816 1816 a, g dbSNP:113980652
1909 1909 a, t dbSNP:778701096
1929 1929 a, g dbSNP:748012386
1983 1983 a, g, t dbSNP:528146538
2026 2026 c, t dbSNP:186728160
2038 2038 a, g dbSNP:773236980
2065 2065 a, g dbSNP:567676558
2102 2102 c, t dbSNP:11551264
2108 2108 -, ac dbSNP:776133409
2109 2109 c, t dbSNP:535429733

Target ORF information:

RefSeq Version XM_005256144
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005256144

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGTTCCCGG GCACCGAGGA GGGGCCTGAA GATGACAGCA CAAAACACGG GGGACGGGTG 
CGCACCTTCC CCCACGAGCG AGGCAACTGG GCCACCCACG TCTATGTACC ATATGAAGCC
AAGGAGGAGT TCCTGGATCT GCTTGATGTG TTGCTGCCCC ATGCCCAGAC ATATGTCCCC
CGGCTGGTAA GGATGAAGGT GTTCCACCTC AGCCTGTCCC AGAGTGTGGT TCTGCGCCAC
CACTGGATCC TCCCCTTCGT GCAGGCTCTG AAAGCCCGTA TGACCTCCTT CCACAGATTC
TTCTTTACTG CCAACCAGGT AAAGATTTAC ACCAATCAAG AGAAAACCAG GACCTTTATT
GGGCTTGAGG TCACTTCAGG GCATGCCCAG TTCCTGGACC TGGTTTCAGA GGTGGACAGA
GTCATGGAGG AATTCAACCT CACCACTTTC TACCAGGATC CTTCTTTCCA CCTCAGCCTG
GCCTGGTGTG TGGGTGATGC ACGTCTCCAG CTGGAGGGGC AGTGCCTGCA GGAACTACAG
GCAATCGTGG ATGGGTTTGA AGATGCTGAG GTGCTGCTGC GCGTGCACAC TGAGCAAGTC
CGCTGCAAGT CTGGGAACAA GTTCTTCTCG ATGCCTTTGA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu49499
Accession Version XM_011523329.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 645bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product U6 snRNA phosphodiesterase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGTTCCCGG GCACCGAGGA GGGGCCTGAA GATGACAGCA CAAAACACGG GGGACGGGTG 
CGCACCTTCC CCCACGAGCG AGGCAACTGG GCCACCCACG TCTATGTACC ATATGAAGCC
AAGGAGGAGT TCCTGGATCT GCTTGATGTG TTGCTGCCCC ATGCCCAGAC ATATGTCCCC
CGGCTGGTAA GGATGAAGGT GTTCCACCTC AGCCTGTCCC AGAGTGTGGT TCTGCGCCAC
CACTGGATCC TCCCCTTCGT GCAGGCTCTG AAAGCCCGTA TGACCTCCTT CCACAGATTC
TTCTTTACTG CCAACCAGGT AAAGATTTAC ACCAATCAAG AGAAAACCAG GACCTTTATT
GGGCTTGAGG TCACTTCAGG GCATGCCCAG TTCCTGGACC TGGTTTCAGA GGTGGACAGA
GTCATGGAGG AATTCAACCT CACCACTTTC TACCAGGATC CTTCTTTCCA CCTCAGCCTG
GCCTGGTGTG TGGGTGATGC ACGTCTCCAG CTGGAGGGGC AGTGCCTGCA GGAACTACAG
GCAATCGTGG ATGGGTTTGA AGATGCTGAG GTGCTGCTGC GCGTGCACAC TGAGCAAGTC
CGCTGCAAGT CTGGGAACAA GTTCTTCTCG ATGCCTTTGA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011521631.1
CDS79..723
Misc Feature(1)79..717(+)
Translation
Position Chain Variation Link
8 8 c, t dbSNP:778859073
25 25 a, g dbSNP:769630452
27 27 c, g, t dbSNP:144716560
29 29 a, c dbSNP:762585706
31 31 a, g dbSNP:138405924
33 33 a, c dbSNP:774385750
34 34 c, t dbSNP:762001177
36 36 c, t dbSNP:767672950
40 40 c, t dbSNP:751037751
49 49 a, t dbSNP:756702350
54 54 c, t dbSNP:766926835
59 59 -, t dbSNP:774728454
73 73 c, t dbSNP:754262096
74 74 c, t dbSNP:755250981
87 87 a, g dbSNP:143792185
89 89 a, g dbSNP:746613694
90 90 c, g, t dbSNP:756831751
94 94 a, g dbSNP:745321157
95 95 a, c dbSNP:769724028
99 99 -, gg dbSNP:759761915
104 104 -, c dbSNP:786205051
113 113 a, t dbSNP:775249170
119 119 a, c dbSNP:749129393
126 126 c, t dbSNP:548754947
127 127 a, g dbSNP:774672635
130 130 a, g dbSNP:762121009
131 131 a, g dbSNP:767791131
133 133 a, c, t dbSNP:531886363
134 134 a, g dbSNP:767016894
138 138 a, g dbSNP:369515724
139 139 c, t dbSNP:567360825
140 140 a, c, g dbSNP:372917091
143 143 c, t dbSNP:751288061
149 149 c, t dbSNP:763524876
151 151 a, c dbSNP:780752842
153 153 c, t dbSNP:549783755
155 155 a, c dbSNP:755570589
156 156 g, t dbSNP:371393773
157 157 a, c dbSNP:374559185
168 168 a, g dbSNP:137853973
169 169 a, g dbSNP:778559247
177 177 c, t dbSNP:147246452
178 178 a, g dbSNP:764580227
180 180 c, t dbSNP:375733174
183 183 c, t dbSNP:199820065
184 184 a, g dbSNP:571564926
188 188 c, g dbSNP:770881616
192 192 c, t dbSNP:771096742
201 201 a, g dbSNP:776583555
215 215 a, t dbSNP:759598117
228 228 a, g dbSNP:770239330
237 237 c, t dbSNP:140760824
242 242 c, t dbSNP:763359090
250 250 -, t dbSNP:754368529
251 251 a, g dbSNP:764413085
252 252 c, t dbSNP:369321909
260 260 a, g dbSNP:142683238
261 261 a, g, t dbSNP:765998448
269 269 a, g dbSNP:35025252
275 275 a, g dbSNP:778763495
283 283 c, t dbSNP:752648429
288 288 c, t dbSNP:758332726
292 292 c, g, t dbSNP:777694507
302 302 a, g dbSNP:757421099
306 306 a, g dbSNP:781440519
312 312 c, g dbSNP:542658449
313 313 c, t dbSNP:769898628
314 314 a, g dbSNP:199886884
327 327 c, g dbSNP:776019365
336 336 c, t dbSNP:749793676
337 337 a, g dbSNP:769067404
348 348 c, g dbSNP:774712588
350 350 a, g dbSNP:762197073
355 355 c, t dbSNP:774644058
356 356 a, g dbSNP:367780234
356 356 -, g dbSNP:762227910
372 372 c, g dbSNP:776322680
376 376 -, ttc dbSNP:755973489
377 377 a, t dbSNP:375204675
386 386 c, t dbSNP:779326318
391 391 a, c dbSNP:748682814
393 393 c, t dbSNP:372875085
395 395 a, g dbSNP:202096477
408 408 c, t dbSNP:747267362
411 411 -, caat dbSNP:777667891
414 414 c, t dbSNP:769596860
423 423 a, c dbSNP:775237878
424 424 a, g dbSNP:139558948
425 425 c, t dbSNP:768253665
426 426 c, t dbSNP:774223335
427 427 a, g dbSNP:137853971
430 430 a, t dbSNP:779896567
437 437 c, t dbSNP:149725439
445 445 c, g dbSNP:768341871
458 458 a, g dbSNP:374426055
460 460 c, t dbSNP:762828282
465 465 c, g dbSNP:761161720
489 489 a, g dbSNP:772079518
493 493 a, g dbSNP:772999682
496 496 a, g dbSNP:760502631
508 508 a, g dbSNP:766156595
509 509 a, c dbSNP:754116399
515 515 a, g dbSNP:377411772
517 517 c, t dbSNP:765307761
518 518 g, t dbSNP:555309420
523 523 a, g dbSNP:758357702
524 524 c, g dbSNP:764715431
531 531 c, t dbSNP:764176381
549 549 c, t dbSNP:371949436
564 564 a, c, g dbSNP:750851175
566 566 a, g dbSNP:146685901
571 571 a, g dbSNP:778666156
580 580 c, t dbSNP:554516072
581 581 a, g dbSNP:757982322
592 592 c, g dbSNP:777276672
596 596 a, g dbSNP:746447180
618 618 a, g dbSNP:770883144
622 622 a, g dbSNP:769032208
624 624 c, t dbSNP:774669193
625 625 a, g dbSNP:762033139
637 637 a, g dbSNP:772272501
641 641 a, g dbSNP:773826400
648 648 c, g dbSNP:761098113
650 650 c, t dbSNP:766841291
654 654 a, g dbSNP:190283472
658 658 c, t dbSNP:182418203
659 659 a, g dbSNP:140289507
660 660 c, t dbSNP:763780991
661 661 a, g dbSNP:751177454
670 670 a, g dbSNP:756831672
673 673 c, g dbSNP:16959641
679 679 a, c, t dbSNP:146419360
680 680 a, g dbSNP:780003077
696 696 c, g dbSNP:149041481
707 707 c, t dbSNP:368558020
708 708 c, g dbSNP:779320272
709 709 a, g dbSNP:143382202
712 712 c, t dbSNP:772360295
713 713 c, t dbSNP:773169264
720 720 a, g dbSNP:760767630
725 725 c, t dbSNP:771359873
727 727 a, c dbSNP:777169639
737 737 c, t dbSNP:759932035
747 747 c, g dbSNP:765579501
763 763 a, g dbSNP:774051099
825 825 a, c dbSNP:772110530
871 871 c, g dbSNP:772987427
889 889 -, tctc dbSNP:370473790
901 901 -, tt dbSNP:759921215
917 917 -, ttct dbSNP:372237467
928 928 -, ctc dbSNP:752765084
931 931 -, ctct dbSNP:200950416
933 933 -, ct dbSNP:762941380
944 944 -, ct dbSNP:751796506
945 945 -, tctg dbSNP:142769438
948 948 -, gtct dbSNP:375560719
949 949 g, t dbSNP:35071272
962 962 -, ct dbSNP:549920112
974 974 c, t dbSNP:575397821
976 976 c, t dbSNP:113112209
977 977 -, tctc dbSNP:547553387
984 984 -, tcctc dbSNP:527401961
1007 1007 -, tctc dbSNP:547883874
1019 1019 c, g, t dbSNP:113876388
1024 1024 -, tg dbSNP:754995890
1025 1025 c, g dbSNP:549064355
1027 1027 c, t dbSNP:565377163
1029 1029 -, ctcctcccctc dbSNP:747135009
1039 1039 -, ct, ctct dbSNP:112874007
1039 1039 -, c dbSNP:369788319
1040 1040 -, ctct dbSNP:72112945
1042 1042 -, ctcttcctctc dbSNP:749283271
1042 1042 -, ctct dbSNP:386384807
1044 1044 -, cctc dbSNP:751375096
1045 1045 -, ctct dbSNP:58782258
1046 1046 c, t dbSNP:151105116
1047 1047 c, t dbSNP:202140498
1052 1052 c, g dbSNP:532682469
1053 1053 -, ct dbSNP:553004572
1065 1065 -, ctc dbSNP:563553918
1068 1068 -, ct dbSNP:747734953
1076 1076 a, t dbSNP:113866163
1099 1099 -, ct dbSNP:139623690
1115 1115 -, ctct dbSNP:565062214
1120 1120 -, tctc dbSNP:201974024
1132 1132 -, ct dbSNP:371978648
1151 1151 c, t dbSNP:547797939
1156 1156 c, t dbSNP:370068353
1161 1161 c, g dbSNP:536035144
1194 1194 c, t dbSNP:559803513
1195 1195 c, g dbSNP:530392108
1215 1215 -, ctct dbSNP:150380411
1217 1217 c, g dbSNP:373066608
1219 1219 a, c dbSNP:548868928
1245 1245 c, g dbSNP:12930821
1255 1255 c, g dbSNP:531016950
1262 1262 c, t dbSNP:552540257
1263 1263 c, t dbSNP:570503152
1283 1283 a, g dbSNP:191811468
1287 1287 a, g dbSNP:183630288
1299 1299 c, t dbSNP:376388970
1301 1301 c, t dbSNP:574116680
1369 1369 c, g dbSNP:185385414
1380 1380 c, t dbSNP:539869982
1387 1387 c, g dbSNP:575497717
1394 1394 a, g dbSNP:545958819
1395 1395 c, t dbSNP:564161180
1396 1396 a, g dbSNP:577377913
1450 1450 c, t dbSNP:768960345
1452 1452 a, c dbSNP:774311786
1462 1462 c, t dbSNP:11551263
1466 1466 c, t dbSNP:765434178
1544 1544 c, g dbSNP:753128270
1568 1568 g, t dbSNP:190241788
1570 1570 a, g dbSNP:182095431
1572 1572 g, t dbSNP:758660280
1607 1607 c, t dbSNP:144654181
1653 1653 c, t dbSNP:62041779
1675 1675 a, g dbSNP:540042603
1677 1677 c, g dbSNP:755679865
1695 1695 a, g dbSNP:552719952
1703 1703 c, t dbSNP:779638358
1708 1708 a, g dbSNP:748960478
1722 1722 c, t dbSNP:148180082
1730 1730 c, g dbSNP:375005880
1755 1755 a, g dbSNP:113980652
1848 1848 a, t dbSNP:778701096
1868 1868 a, g dbSNP:748012386
1922 1922 a, g, t dbSNP:528146538
1965 1965 c, t dbSNP:186728160
1977 1977 a, g dbSNP:773236980
2004 2004 a, g dbSNP:567676558
2041 2041 c, t dbSNP:11551264
2047 2047 -, ac dbSNP:776133409
2048 2048 c, t dbSNP:535429733

Target ORF information:

RefSeq Version XM_011523329
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011523329

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGTTCCCGG GCACCGAGGA GGGGCCTGAA GATGACAGCA CAAAACACGG GGGACGGGTG 
CGCACCTTCC CCCACGAGCG AGGCAACTGG GCCACCCACG TCTATGTACC ATATGAAGCC
AAGGAGGAGT TCCTGGATCT GCTTGATGTG TTGCTGCCCC ATGCCCAGAC ATATGTCCCC
CGGCTGGTAA GGATGAAGGT GTTCCACCTC AGCCTGTCCC AGAGTGTGGT TCTGCGCCAC
CACTGGATCC TCCCCTTCGT GCAGGCTCTG AAAGCCCGTA TGACCTCCTT CCACAGATTC
TTCTTTACTG CCAACCAGGT AAAGATTTAC ACCAATCAAG AGAAAACCAG GACCTTTATT
GGGCTTGAGG TCACTTCAGG GCATGCCCAG TTCCTGGACC TGGTTTCAGA GGTGGACAGA
GTCATGGAGG AATTCAACCT CACCACTTTC TACCAGGATC CTTCTTTCCA CCTCAGCCTG
GCCTGGTGTG TGGGTGATGC ACGTCTCCAG CTGGAGGGGC AGTGCCTGCA GGAACTACAG
GCAATCGTGG ATGGGTTTGA AGATGCTGAG GTGCTGCTGC GCGTGCACAC TGAGCAAGTC
CGCTGCAAGT CTGGGAACAA GTTCTTCTCG ATGCCTTTGA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71970
Accession Version XM_011523330.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 492bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product U6 snRNA phosphodiesterase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGAGCGCGG CGCCCCTGGT GGGCTACAGC AGCAGCGGCT CCGAGGATGA GTCCGAGGAC 
GGGATGCGGA CCAGGCCGGG GGATGGGAGC CACCGTCGTG GCCAGAGCCC CCTTCCCAGG
CAGAGATTTC CAGTACCTGA CAGTGTGCTG AACATGTTCC CGGGCACCGA GGAGGGGCCT
GAAGATGACA GCACAAAACA CGGGGGACGG GTGCGCACCT TCCCCCACGA GCGAGGCAAC
TGGGCCACCC ACGTCTATGT ACCATATGAA GCCAAGGAGG AGTTCCTGGA TCTGCTTGAT
GTGTTGCTGC CCCATGCCCA GACATATGTC CCCCGGCTGG TAAGGATGAA GGTGTTCCAC
CTCAGCCTGT CCCAGAGTGT GGTTCTGCGC CACCACTGGA TCCTCCCCTT CGTGCAGGCT
CTGAAAGCCC GTATGACCTC CTTCCACAGG TGCCAGAAGC CCATGCCAGA AGCCCAACCC
AAAGTGGCTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011521632.1
CDS78..569
Misc Feature(1)207..>518(+)
Translation
Position Chain Variation Link
7 7 a, g dbSNP:749839080
8 8 a, g dbSNP:557453429
15 15 g, t dbSNP:572460232
21 21 c, t dbSNP:539851929
25 25 g, t dbSNP:555381360
27 27 c, g dbSNP:73546949
29 29 c, t dbSNP:755207534
30 30 a, g dbSNP:544521629
32 32 g, t dbSNP:748260509
41 41 c, g dbSNP:758458788
44 44 a, g dbSNP:562526527
48 48 a, g dbSNP:778195515
49 49 a, c dbSNP:747515828
52 52 a, c, g dbSNP:201250414
58 58 c, g dbSNP:746174189
65 65 a, t dbSNP:768425672
66 66 g, t dbSNP:774106572
67 67 a, c, g dbSNP:543492919
70 70 a, g dbSNP:370555892
73 73 g, t dbSNP:773091216
78 78 a, g dbSNP:760648279
80 80 c, g dbSNP:546288690
83 83 c, t dbSNP:753636337
90 90 -, c dbSNP:781151070
90 90 c, t dbSNP:759290159
92 92 c, t dbSNP:765469310
97 97 c, t dbSNP:373657742
100 100 a, g dbSNP:564572047
107 107 c, g dbSNP:777899627
108 108 a, t dbSNP:752060249
115 115 a, g dbSNP:757792250
119 119 a, c dbSNP:3743559
131 131 c, g dbSNP:746264362
133 133 a, g dbSNP:770232543
138 138 a, g dbSNP:778756392
138 138 -, g dbSNP:747958554
144 144 c, t dbSNP:747805446
145 145 c, g dbSNP:771539759
151 151 c, g dbSNP:772792606
155 155 c, g dbSNP:375706004
157 157 c, g dbSNP:770934785
167 167 a, c dbSNP:149411375
174 174 c, g dbSNP:759378341
177 177 a, g dbSNP:769630452
179 179 c, g, t dbSNP:144716560
181 181 a, c dbSNP:762585706
183 183 a, g dbSNP:138405924
185 185 a, c dbSNP:774385750
186 186 c, t dbSNP:762001177
188 188 c, t dbSNP:767672950
192 192 c, t dbSNP:751037751
201 201 a, t dbSNP:756702350
206 206 c, t dbSNP:766926835
211 211 -, t dbSNP:774728454
225 225 c, t dbSNP:754262096
226 226 c, t dbSNP:755250981
239 239 a, g dbSNP:143792185
241 241 a, g dbSNP:746613694
242 242 c, g, t dbSNP:756831751
246 246 a, g dbSNP:745321157
247 247 a, c dbSNP:769724028
251 251 -, gg dbSNP:759761915
256 256 -, c dbSNP:786205051
265 265 a, t dbSNP:775249170
271 271 a, c dbSNP:749129393
278 278 c, t dbSNP:548754947
279 279 a, g dbSNP:774672635
282 282 a, g dbSNP:762121009
283 283 a, g dbSNP:767791131
285 285 a, c, t dbSNP:531886363
286 286 a, g dbSNP:767016894
290 290 a, g dbSNP:369515724
291 291 c, t dbSNP:567360825
292 292 a, c, g dbSNP:372917091
295 295 c, t dbSNP:751288061
301 301 c, t dbSNP:763524876
303 303 a, c dbSNP:780752842
305 305 c, t dbSNP:549783755
307 307 a, c dbSNP:755570589
308 308 g, t dbSNP:371393773
309 309 a, c dbSNP:374559185
320 320 a, g dbSNP:137853973
321 321 a, g dbSNP:778559247
329 329 c, t dbSNP:147246452
330 330 a, g dbSNP:764580227
332 332 c, t dbSNP:375733174
335 335 c, t dbSNP:199820065
336 336 a, g dbSNP:571564926
340 340 c, g dbSNP:770881616
344 344 c, t dbSNP:771096742
353 353 a, g dbSNP:776583555
367 367 a, t dbSNP:759598117
380 380 a, g dbSNP:770239330
389 389 c, t dbSNP:140760824
394 394 c, t dbSNP:763359090
402 402 -, t dbSNP:754368529
403 403 a, g dbSNP:764413085
404 404 c, t dbSNP:369321909
412 412 a, g dbSNP:142683238
413 413 a, g, t dbSNP:765998448
421 421 a, g dbSNP:35025252
427 427 a, g dbSNP:778763495
435 435 c, t dbSNP:752648429
440 440 c, t dbSNP:758332726
444 444 c, g, t dbSNP:777694507
454 454 a, g dbSNP:757421099
458 458 a, g dbSNP:781440519
464 464 c, g dbSNP:542658449
465 465 c, t dbSNP:769898628
466 466 a, g dbSNP:199886884
479 479 c, g dbSNP:776019365
488 488 c, t dbSNP:749793676
489 489 a, g dbSNP:769067404
500 500 c, g dbSNP:774712588
502 502 a, g dbSNP:762197073
507 507 c, t dbSNP:774644058
508 508 a, g dbSNP:367780234
508 508 -, g dbSNP:762227910
524 524 c, g dbSNP:776322680
540 540 a, g dbSNP:577392993
549 549 -, g dbSNP:751069680
610 610 a, g dbSNP:776211262
636 636 c, t dbSNP:574907628
641 641 c, t dbSNP:745720455
688 688 a, c dbSNP:755883844
752 752 c, t dbSNP:150960523
783 783 a, c dbSNP:563249604
788 788 c, g dbSNP:780008370
796 796 c, t dbSNP:534888599
826 826 g, t dbSNP:530751846
844 844 c, g dbSNP:553741946
852 852 c, t dbSNP:369096616
878 878 a, g dbSNP:145819484
879 879 g, t dbSNP:532468493
882 882 c, t dbSNP:768806126
888 888 a, c, g dbSNP:547894656
899 899 c, g dbSNP:748411023
934 934 a, g dbSNP:185762636
969 969 a, g dbSNP:772236772
978 978 a, g dbSNP:773541347
988 988 c, t dbSNP:566032187
999 999 c, t dbSNP:141472399
1014 1014 c, t dbSNP:548644328
1054 1054 c, t dbSNP:570362291
1055 1055 c, g dbSNP:537768544
1072 1072 c, t dbSNP:766807078
1073 1073 a, g dbSNP:542058856
1075 1075 a, g dbSNP:777298198
1082 1082 g, t dbSNP:759901009
1112 1112 a, c dbSNP:558868700
1113 1113 a, c dbSNP:571040358
1170 1170 c, t dbSNP:534392869
1204 1204 c, g dbSNP:763664239
1247 1247 a, g dbSNP:553032924
1327 1327 c, t dbSNP:751096518
1415 1415 c, t dbSNP:574051387
1416 1416 a, g dbSNP:756779107
1523 1523 a, g dbSNP:73546966
1573 1573 g, t dbSNP:750048875
1649 1649 a, g dbSNP:572159271
1651 1651 g, t dbSNP:115330942

Target ORF information:

RefSeq Version XM_011523330
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011523330

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGAGCGCGG CGCCCCTGGT GGGCTACAGC AGCAGCGGCT CCGAGGATGA GTCCGAGGAC 
GGGATGCGGA CCAGGCCGGG GGATGGGAGC CACCGTCGTG GCCAGAGCCC CCTTCCCAGG
CAGAGATTTC CAGTACCTGA CAGTGTGCTG AACATGTTCC CGGGCACCGA GGAGGGGCCT
GAAGATGACA GCACAAAACA CGGGGGACGG GTGCGCACCT TCCCCCACGA GCGAGGCAAC
TGGGCCACCC ACGTCTATGT ACCATATGAA GCCAAGGAGG AGTTCCTGGA TCTGCTTGAT
GTGTTGCTGC CCCATGCCCA GACATATGTC CCCCGGCTGG TAAGGATGAA GGTGTTCCAC
CTCAGCCTGT CCCAGAGTGT GGTTCTGCGC CACCACTGGA TCCTCCCCTT CGTGCAGGCT
CTGAAAGCCC GTATGACCTC CTTCCACAGG TGCCAGAAGC CCATGCCAGA AGCCCAACCC
AAAGTGGCTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu08584
Accession Version NM_001204911.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 561bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product U6 snRNA phosphodiesterase isoform 3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC380401.1, AK301494.1, AC010543.9, BF733890.1, BQ223186.1, BX117268.1, BI910866.1 and DB382938.1. Summary: This gene encodes a protein with several conserved domains, however, its exact function is not known. Mutations in this gene are associated with poikiloderma with neutropenia (PN), which shows phenotypic overlap with Rothmund-Thomson syndrome (RTS) caused by mutations in the RECQL4 gene. It is believed that this gene product interacts with RECQL4 protein via SMAD4 proteins, explaining the partial clinical overlap between PN and RTS. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]. Transcript Variant: This variant (3) lacks several exons from the 3' end, and contains an alternate 3' terminal exon compared to variant 1. This results in a shorter isofom (3) with a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301494.1, CN259877.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGAGCGCGG CGCCCCTGGT GGGCTACAGC AGCAGCGGCT CCGAGGATGA GTCCGAGGAC 
GGGATGCGGA CCAGGCCGGG GGATGGGAGC CACCGTCGTG GCCAGAGCCC CCTTCCCAGG
CAGAGATTTC CAGTACCTGA CAGTGTGCTG AACATGTTCC CGGGCACCGA GGAGGGGCCT
GAAGATGACA GCACAAAACA CGGGGGACGG GTGCGCACCT TCCCCCACGA GCGAGGCAAC
TGGGCCACCC ACGTCTATGT ACCATATGAA GCCAAGGAGG AGTTCCTGGA TCTGCTTGAT
GTGTTGCTGC CCCATGCCCA GACATATGTC CCCCGGCTGG TAAGGATGAA GGTGTTCCAC
CTCAGCCTGT CCCAGAGTGT GGTTCTGCGC CACCACTGGA TCCTCCCCTT CGTGCAGGCT
CTGAAAGCCC GTATGACCTC CTTCCACAGC CCCCATCCAG GACCTCATTG CCTCATTGGA
ACCAAAGATG CCCCTGTCAC CCAGGAAATT CCAAAGGATT TAGGAGCTCT GCGTCAGGAG
CCAGGATCAC AGACCAGATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001191840.1
CDS112..672
Misc Feature(1)103..105(+)
Misc Feature(2)241..>552(+)
Exon (1)1..209
Gene:USB1
Gene Synonym:
Exon (2)210..376
Gene:USB1
Gene Synonym:
Exon (3)377..560
Gene:USB1
Gene Synonym:
Exon (4)561..3113
Gene:USB1
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, t dbSNP:539113150
41 41 a, g dbSNP:749839080
42 42 a, g dbSNP:557453429
49 49 g, t dbSNP:572460232
55 55 c, t dbSNP:539851929
59 59 g, t dbSNP:555381360
61 61 c, g dbSNP:73546949
63 63 c, t dbSNP:755207534
64 64 a, g dbSNP:544521629
66 66 g, t dbSNP:748260509
75 75 c, g dbSNP:758458788
78 78 a, g dbSNP:562526527
82 82 a, g dbSNP:778195515
83 83 a, c dbSNP:747515828
86 86 a, c, g dbSNP:201250414
92 92 c, g dbSNP:746174189
99 99 a, t dbSNP:768425672
100 100 g, t dbSNP:774106572
101 101 a, c, g dbSNP:543492919
104 104 a, g dbSNP:370555892
107 107 g, t dbSNP:773091216
112 112 a, g dbSNP:760648279
114 114 c, g dbSNP:546288690
117 117 c, t dbSNP:753636337
124 124 -, c dbSNP:781151070
124 124 c, t dbSNP:759290159
126 126 c, t dbSNP:765469310
131 131 c, t dbSNP:373657742
134 134 a, g dbSNP:564572047
141 141 c, g dbSNP:777899627
142 142 a, t dbSNP:752060249
149 149 a, g dbSNP:757792250
153 153 a, c dbSNP:3743559
165 165 c, g dbSNP:746264362
167 167 a, g dbSNP:770232543
172 172 a, g dbSNP:778756392
172 172 -, g dbSNP:747958554
178 178 c, t dbSNP:747805446
179 179 c, g dbSNP:771539759
185 185 c, g dbSNP:772792606
189 189 c, g dbSNP:375706004
191 191 c, g dbSNP:770934785
201 201 a, c dbSNP:149411375
208 208 c, g dbSNP:759378341
211 211 a, g dbSNP:769630452
213 213 c, g, t dbSNP:144716560
215 215 a, c dbSNP:762585706
217 217 a, g dbSNP:138405924
219 219 a, c dbSNP:774385750
220 220 c, t dbSNP:762001177
222 222 c, t dbSNP:767672950
226 226 c, t dbSNP:751037751
235 235 a, t dbSNP:756702350
240 240 c, t dbSNP:766926835
245 245 -, t dbSNP:774728454
259 259 c, t dbSNP:754262096
260 260 c, t dbSNP:755250981
273 273 a, g dbSNP:143792185
275 275 a, g dbSNP:746613694
276 276 c, g, t dbSNP:756831751
280 280 a, g dbSNP:745321157
281 281 a, c dbSNP:769724028
285 285 -, gg dbSNP:759761915
290 290 -, c dbSNP:786205051
299 299 a, t dbSNP:775249170
305 305 a, c dbSNP:749129393
312 312 c, t dbSNP:548754947
313 313 a, g dbSNP:774672635
316 316 a, g dbSNP:762121009
317 317 a, g dbSNP:767791131
319 319 a, c, t dbSNP:531886363
320 320 a, g dbSNP:767016894
324 324 a, g dbSNP:369515724
325 325 c, t dbSNP:567360825
326 326 a, c, g dbSNP:372917091
329 329 c, t dbSNP:751288061
335 335 c, t dbSNP:763524876
337 337 a, c dbSNP:780752842
339 339 c, t dbSNP:549783755
341 341 a, c dbSNP:755570589
342 342 g, t dbSNP:371393773
343 343 a, c dbSNP:374559185
354 354 a, g dbSNP:137853973
355 355 a, g dbSNP:778559247
363 363 c, t dbSNP:147246452
364 364 a, g dbSNP:764580227
366 366 c, t dbSNP:375733174
369 369 c, t dbSNP:199820065
370 370 a, g dbSNP:571564926
374 374 c, g dbSNP:770881616
378 378 c, t dbSNP:771096742
387 387 a, g dbSNP:776583555
401 401 a, t dbSNP:759598117
414 414 a, g dbSNP:770239330
423 423 c, t dbSNP:140760824
428 428 c, t dbSNP:763359090
436 436 -, t dbSNP:754368529
437 437 a, g dbSNP:764413085
438 438 c, t dbSNP:369321909
446 446 a, g dbSNP:142683238
447 447 a, g, t dbSNP:765998448
455 455 a, g dbSNP:35025252
461 461 a, g dbSNP:778763495
469 469 c, t dbSNP:752648429
474 474 c, t dbSNP:758332726
478 478 c, g, t dbSNP:777694507
488 488 a, g dbSNP:757421099
492 492 a, g dbSNP:781440519
498 498 c, g dbSNP:542658449
499 499 c, t dbSNP:769898628
500 500 a, g dbSNP:199886884
513 513 c, g dbSNP:776019365
522 522 c, t dbSNP:749793676
523 523 a, g dbSNP:769067404
534 534 c, g dbSNP:774712588
536 536 a, g dbSNP:762197073
541 541 c, t dbSNP:774644058
542 542 a, g dbSNP:367780234
542 542 -, g dbSNP:762227910
558 558 c, g dbSNP:776322680
590 590 c, g dbSNP:547631764
609 609 -, gaca dbSNP:765938774
643 643 c, t dbSNP:528244148
644 644 a, g dbSNP:748510212
656 656 a, g dbSNP:746601154
731 731 g, t dbSNP:550122238
740 740 c, t dbSNP:559377650
788 788 c, t dbSNP:770779855
804 804 c, t dbSNP:192949662
820 820 a, t dbSNP:184209364
843 843 g, t dbSNP:547959717
847 847 a, g dbSNP:142772054
887 887 a, g dbSNP:202096926
893 893 a, g dbSNP:535300210
897 897 a, c dbSNP:780792646
911 911 a, g dbSNP:537911808
921 921 a, g dbSNP:549882366
924 924 a, t dbSNP:745809938
926 926 c, t dbSNP:571567934
930 930 a, g dbSNP:769789560
933 933 c, t dbSNP:538662605
942 942 c, t dbSNP:186671472
1011 1011 a, g dbSNP:200358845
1039 1039 c, g dbSNP:760815165
1041 1041 c, t dbSNP:548617254
1045 1045 c, t dbSNP:16959635
1046 1046 a, g dbSNP:536983136
1075 1075 c, g dbSNP:555169364
1087 1087 a, c dbSNP:576733215
1088 1088 a, g dbSNP:146045932
1099 1099 g, t dbSNP:546899921
1108 1108 a, g dbSNP:1986162
1114 1114 c, t dbSNP:535121234
1181 1181 c, t dbSNP:576912863
1184 1184 c, t dbSNP:540665164
1208 1208 a, c dbSNP:550157857
1214 1214 a, g dbSNP:568468893
1227 1227 c, t dbSNP:140205720
1230 1230 a, g dbSNP:143885155
1247 1247 a, c dbSNP:576853518
1248 1248 a, c dbSNP:541736716
1270 1270 c, g dbSNP:563111809
1287 1287 c, t dbSNP:192342616
1305 1305 c, t dbSNP:549820961
1337 1337 a, g dbSNP:370588227
1360 1360 a, g dbSNP:538119631
1368 1368 c, t dbSNP:145828902
1391 1391 a, t dbSNP:767941642
1422 1422 c, t dbSNP:532669267
1444 1444 a, g dbSNP:184676356
1473 1473 c, t dbSNP:566082848
1474 1474 c, t dbSNP:773776127
1482 1482 a, g dbSNP:761103063
1486 1486 a, g dbSNP:767039246
1498 1498 a, g dbSNP:77670243
1515 1515 c, t dbSNP:555304417
1550 1550 a, g dbSNP:181653350
1568 1568 g, t dbSNP:185426465
1580 1580 c, t dbSNP:537633419
1589 1589 a, g dbSNP:194102
1607 1607 c, g dbSNP:194101
1628 1628 c, t dbSNP:758011886
1683 1683 c, t dbSNP:148565907
1714 1714 c, t dbSNP:763461960
1744 1744 a, t dbSNP:142906324
1750 1750 g, t dbSNP:776874061
1756 1756 a, g dbSNP:9938499
1766 1766 c, g dbSNP:552659751
1802 1802 c, g dbSNP:189432686
1813 1813 c, g dbSNP:194100
1848 1848 a, t dbSNP:756792576
1858 1858 c, t dbSNP:181477052
1877 1877 a, g dbSNP:577392993
1886 1886 -, g dbSNP:751069680
1947 1947 a, g dbSNP:776211262
1973 1973 c, t dbSNP:574907628
1978 1978 c, t dbSNP:745720455
2025 2025 a, c dbSNP:755883844
2089 2089 c, t dbSNP:150960523
2120 2120 a, c dbSNP:563249604
2125 2125 c, g dbSNP:780008370
2133 2133 c, t dbSNP:534888599
2163 2163 g, t dbSNP:530751846
2181 2181 c, g dbSNP:553741946
2189 2189 c, t dbSNP:369096616
2215 2215 a, g dbSNP:145819484
2216 2216 g, t dbSNP:532468493
2219 2219 c, t dbSNP:768806126
2225 2225 a, c, g dbSNP:547894656
2236 2236 c, g dbSNP:748411023
2271 2271 a, g dbSNP:185762636
2306 2306 a, g dbSNP:772236772
2315 2315 a, g dbSNP:773541347
2325 2325 c, t dbSNP:566032187
2336 2336 c, t dbSNP:141472399
2351 2351 c, t dbSNP:548644328
2391 2391 c, t dbSNP:570362291
2392 2392 c, g dbSNP:537768544
2409 2409 c, t dbSNP:766807078
2410 2410 a, g dbSNP:542058856
2412 2412 a, g dbSNP:777298198
2419 2419 g, t dbSNP:759901009
2449 2449 a, c dbSNP:558868700
2450 2450 a, c dbSNP:571040358
2507 2507 c, t dbSNP:534392869
2541 2541 c, g dbSNP:763664239
2584 2584 a, g dbSNP:553032924
2664 2664 c, t dbSNP:751096518
2752 2752 c, t dbSNP:574051387
2753 2753 a, g dbSNP:756779107
2860 2860 a, g dbSNP:73546966
2910 2910 g, t dbSNP:750048875
2986 2986 a, g dbSNP:572159271
2988 2988 g, t dbSNP:115330942

Target ORF information:

RefSeq Version NM_001204911
Organism Homo sapiens (human)
Definition Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204911

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGAGCGCGG CGCCCCTGGT GGGCTACAGC AGCAGCGGCT CCGAGGATGA GTCCGAGGAC 
GGGATGCGGA CCAGGCCGGG GGATGGGAGC CACCGTCGTG GCCAGAGCCC CCTTCCCAGG
CAGAGATTTC CAGTACCTGA CAGTGTGCTG AACATGTTCC CGGGCACCGA GGAGGGGCCT
GAAGATGACA GCACAAAACA CGGGGGACGG GTGCGCACCT TCCCCCACGA GCGAGGCAAC
TGGGCCACCC ACGTCTATGT ACCATATGAA GCCAAGGAGG AGTTCCTGGA TCTGCTTGAT
GTGTTGCTGC CCCATGCCCA GACATATGTC CCCCGGCTGG TAAGGATGAA GGTGTTCCAC
CTCAGCCTGT CCCAGAGTGT GGTTCTGCGC CACCACTGGA TCCTCCCCTT CGTGCAGGCT
CTGAAAGCCC GTATGACCTC CTTCCACAGC CCCCATCCAG GACCTCATTG CCTCATTGGA
ACCAAAGATG CCCCTGTCAC CCAGGAAATT CCAAAGGATT TAGGAGCTCT GCGTCAGGAG
CCAGGATCAC AGACCAGATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20716
Accession Version NM_024598.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 798bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product U6 snRNA phosphodiesterase isoform 1
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC363309.1, BC004415.1, AC012182.7 and DB364698.1. This sequence is a reference standard in the RefSeqGene project. On Sep 3, 2010 this sequence version replaced gi:42716282. Summary: This gene encodes a protein with several conserved domains, however, its exact function is not known. Mutations in this gene are associated with poikiloderma with neutropenia (PN), which shows phenotypic overlap with Rothmund-Thomson syndrome (RTS) caused by mutations in the RECQL4 gene. It is believed that this gene product interacts with RECQL4 protein via SMAD4 proteins, explaining the partial clinical overlap between PN and RTS. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]. Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK023216.1, BC004415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGAGCGCGG CGCCCCTGGT GGGCTACAGC AGCAGCGGCT CCGAGGATGA GTCCGAGGAC 
GGGATGCGGA CCAGGCCGGG GGATGGGAGC CACCGTCGTG GCCAGAGCCC CCTTCCCAGG
CAGAGATTTC CAGTACCTGA CAGTGTGCTG AACATGTTCC CGGGCACCGA GGAGGGGCCT
GAAGATGACA GCACAAAACA CGGGGGACGG GTGCGCACCT TCCCCCACGA GCGAGGCAAC
TGGGCCACCC ACGTCTATGT ACCATATGAA GCCAAGGAGG AGTTCCTGGA TCTGCTTGAT
GTGTTGCTGC CCCATGCCCA GACATATGTC CCCCGGCTGG TAAGGATGAA GGTGTTCCAC
CTCAGCCTGT CCCAGAGTGT GGTTCTGCGC CACCACTGGA TCCTCCCCTT CGTGCAGGCT
CTGAAAGCCC GTATGACCTC CTTCCACAGA TTCTTCTTTA CTGCCAACCA GGTAAAGATT
TACACCAATC AAGAGAAAAC CAGGACCTTT ATTGGGCTTG AGGTCACTTC AGGGCATGCC
CAGTTCCTGG ACCTGGTTTC AGAGGTGGAC AGAGTCATGG AGGAATTCAA CCTCACCACT
TTCTACCAGG ATCCTTCTTT CCACCTCAGC CTGGCCTGGT GTGTGGGTGA TGCACGTCTC
CAGCTGGAGG GGCAGTGCCT GCAGGAACTA CAGGCAATCG TGGATGGGTT TGAAGATGCT
GAGGTGCTGC TGCGCGTGCA CACTGAGCAA GTCCGCTGCA AGTCTGGGAA CAAGTTCTTC
TCGATGCCTT TGAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_078874.2
CDS112..909
Misc Feature(1)103..105(+)
Misc Feature(2)241..903(+)
Exon (1)1..209
Gene:USB1
Gene Synonym:
Exon (2)210..376
Gene:USB1
Gene Synonym:
Exon (3)377..560
Gene:USB1
Gene Synonym:
Exon (4)561..614
Gene:USB1
Gene Synonym:
Exon (5)615..720
Gene:USB1
Gene Synonym:
Exon (6)721..804
Gene:USB1
Gene Synonym:
Exon (7)805..2287
Gene:USB1
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, t dbSNP:539113150
41 41 a, g dbSNP:749839080
42 42 a, g dbSNP:557453429
49 49 g, t dbSNP:572460232
55 55 c, t dbSNP:539851929
59 59 g, t dbSNP:555381360
61 61 c, g dbSNP:73546949
63 63 c, t dbSNP:755207534
64 64 a, g dbSNP:544521629
66 66 g, t dbSNP:748260509
75 75 c, g dbSNP:758458788
78 78 a, g dbSNP:562526527
82 82 a, g dbSNP:778195515
83 83 a, c dbSNP:747515828
86 86 a, c, g dbSNP:201250414
92 92 c, g dbSNP:746174189
99 99 a, t dbSNP:768425672
100 100 g, t dbSNP:774106572
101 101 a, c, g dbSNP:543492919
104 104 a, g dbSNP:370555892
107 107 g, t dbSNP:773091216
112 112 a, g dbSNP:760648279
114 114 c, g dbSNP:546288690
117 117 c, t dbSNP:753636337
124 124 -, c dbSNP:781151070
124 124 c, t dbSNP:759290159
126 126 c, t dbSNP:765469310
131 131 c, t dbSNP:373657742
134 134 a, g dbSNP:564572047
141 141 c, g dbSNP:777899627
142 142 a, t dbSNP:752060249
149 149 a, g dbSNP:757792250
153 153 a, c dbSNP:3743559
165 165 c, g dbSNP:746264362
167 167 a, g dbSNP:770232543
172 172 a, g dbSNP:778756392
172 172 -, g dbSNP:747958554
178 178 c, t dbSNP:747805446
179 179 c, g dbSNP:771539759
185 185 c, g dbSNP:772792606
189 189 c, g dbSNP:375706004
191 191 c, g dbSNP:770934785
201 201 a, c dbSNP:149411375
208 208 c, g dbSNP:759378341
211 211 a, g dbSNP:769630452
213 213 c, g, t dbSNP:144716560
215 215 a, c dbSNP:762585706
217 217 a, g dbSNP:138405924
219 219 a, c dbSNP:774385750
220 220 c, t dbSNP:762001177
222 222 c, t dbSNP:767672950
226 226 c, t dbSNP:751037751
235 235 a, t dbSNP:756702350
240 240 c, t dbSNP:766926835
245 245 -, t dbSNP:774728454
259 259 c, t dbSNP:754262096
260 260 c, t dbSNP:755250981
273 273 a, g dbSNP:143792185
275 275 a, g dbSNP:746613694
276 276 c, g, t dbSNP:756831751
280 280 a, g dbSNP:745321157
281 281 a, c dbSNP:769724028
285 285 -, gg dbSNP:759761915
290 290 -, c dbSNP:786205051
299 299 a, t dbSNP:775249170
305 305 a, c dbSNP:749129393
312 312 c, t dbSNP:548754947
313 313 a, g dbSNP:774672635
316 316 a, g dbSNP:762121009
317 317 a, g dbSNP:767791131
319 319 a, c, t dbSNP:531886363
320 320 a, g dbSNP:767016894
324 324 a, g dbSNP:369515724
325 325 c, t dbSNP:567360825
326 326 a, c, g dbSNP:372917091
329 329 c, t dbSNP:751288061
335 335 c, t dbSNP:763524876
337 337 a, c dbSNP:780752842
339 339 c, t dbSNP:549783755
341 341 a, c dbSNP:755570589
342 342 g, t dbSNP:371393773
343 343 a, c dbSNP:374559185
354 354 a, g dbSNP:137853973
355 355 a, g dbSNP:778559247
363 363 c, t dbSNP:147246452
364 364 a, g dbSNP:764580227
366 366 c, t dbSNP:375733174
369 369 c, t dbSNP:199820065
370 370 a, g dbSNP:571564926
374 374 c, g dbSNP:770881616
378 378 c, t dbSNP:771096742
387 387 a, g dbSNP:776583555
401 401 a, t dbSNP:759598117
414 414 a, g dbSNP:770239330
423 423 c, t dbSNP:140760824
428 428 c, t dbSNP:763359090
436 436 -, t dbSNP:754368529
437 437 a, g dbSNP:764413085
438 438 c, t dbSNP:369321909
446 446 a, g dbSNP:142683238
447 447 a, g, t dbSNP:765998448
455 455 a, g dbSNP:35025252
461 461 a, g dbSNP:778763495
469 469 c, t dbSNP:752648429
474 474 c, t dbSNP:758332726
478 478 c, g, t dbSNP:777694507
488 488 a, g dbSNP:757421099
492 492 a, g dbSNP:781440519
498 498 c, g dbSNP:542658449
499 499 c, t dbSNP:769898628
500 500 a, g dbSNP:199886884
513 513 c, g dbSNP:776019365
522 522 c, t dbSNP:749793676
523 523 a, g dbSNP:769067404
534 534 c, g dbSNP:774712588
536 536 a, g dbSNP:762197073
541 541 c, t dbSNP:774644058
542 542 a, g dbSNP:367780234
542 542 -, g dbSNP:762227910
558 558 c, g dbSNP:776322680
562 562 -, ttc dbSNP:755973489
563 563 a, t dbSNP:375204675
572 572 c, t dbSNP:779326318
577 577 a, c dbSNP:748682814
579 579 c, t dbSNP:372875085
581 581 a, g dbSNP:202096477
594 594 c, t dbSNP:747267362
597 597 -, caat dbSNP:777667891
600 600 c, t dbSNP:769596860
609 609 a, c dbSNP:775237878
610 610 a, g dbSNP:139558948
611 611 c, t dbSNP:768253665
612 612 c, t dbSNP:774223335
613 613 a, g dbSNP:137853971
616 616 a, t dbSNP:779896567
623 623 c, t dbSNP:149725439
631 631 c, g dbSNP:768341871
644 644 a, g dbSNP:374426055
646 646 c, t dbSNP:762828282
651 651 c, g dbSNP:761161720
675 675 a, g dbSNP:772079518
679 679 a, g dbSNP:772999682
682 682 a, g dbSNP:760502631
694 694 a, g dbSNP:766156595
695 695 a, c dbSNP:754116399
701 701 a, g dbSNP:377411772
703 703 c, t dbSNP:765307761
704 704 g, t dbSNP:555309420
709 709 a, g dbSNP:758357702
710 710 c, g dbSNP:764715431
717 717 c, t dbSNP:764176381
735 735 c, t dbSNP:371949436
750 750 a, c, g dbSNP:750851175
752 752 a, g dbSNP:146685901
757 757 a, g dbSNP:778666156
766 766 c, t dbSNP:554516072
767 767 a, g dbSNP:757982322
778 778 c, g dbSNP:777276672
782 782 a, g dbSNP:746447180
804 804 a, g dbSNP:770883144
808 808 a, g dbSNP:769032208
810 810 c, t dbSNP:774669193
811 811 a, g dbSNP:762033139
823 823 a, g dbSNP:772272501
827 827 a, g dbSNP:773826400
834 834 c, g dbSNP:761098113
836 836 c, t dbSNP:766841291
840 840 a, g dbSNP:190283472
844 844 c, t dbSNP:182418203
845 845 a, g dbSNP:140289507
846 846 c, t dbSNP:763780991
847 847 a, g dbSNP:751177454
856 856 a, g dbSNP:756831672
859 859 c, g dbSNP:16959641
865 865 a, c, t dbSNP:146419360
866 866 a, g dbSNP:780003077
882 882 c, g dbSNP:149041481
893 893 c, t dbSNP:368558020
894 894 c, g dbSNP:779320272
895 895 a, g dbSNP:143382202
898 898 c, t dbSNP:772360295
899 899 c, t dbSNP:773169264
906 906 a, g dbSNP:760767630
911 911 c, t dbSNP:771359873
913 913 a, c dbSNP:777169639
923 923 c, t dbSNP:759932035
933 933 c, g dbSNP:765579501
949 949 a, g dbSNP:774051099
1011 1011 a, c dbSNP:772110530
1057 1057 c, g dbSNP:772987427
1075 1075 -, tctc dbSNP:370473790
1087 1087 -, tt dbSNP:759921215
1103 1103 -, ttct dbSNP:372237467
1114 1114 -, ctc dbSNP:752765084
1117 1117 -, ctct dbSNP:200950416
1119 1119 -, ct dbSNP:762941380
1130 1130 -, ct dbSNP:751796506
1131 1131 -, tctg dbSNP:142769438
1134 1134 -, gtct dbSNP:375560719
1135 1135 g, t dbSNP:35071272
1148 1148 -, ct dbSNP:549920112
1160 1160 c, t dbSNP:575397821
1162 1162 c, t dbSNP:113112209
1163 1163 -, tctc dbSNP:547553387
1170 1170 -, tcctc dbSNP:527401961
1193 1193 -, tctc dbSNP:547883874
1205 1205 c, g, t dbSNP:113876388
1210 1210 -, tg dbSNP:754995890
1211 1211 c, g dbSNP:549064355
1213 1213 c, t dbSNP:565377163
1215 1215 -, ctcctcccctc dbSNP:747135009
1225 1225 -, ct, ctct dbSNP:112874007
1225 1225 -, c dbSNP:369788319
1226 1226 -, ctct dbSNP:72112945
1228 1228 -, ctcttcctctc dbSNP:749283271
1228 1228 -, ctct dbSNP:386384807
1230 1230 -, cctc dbSNP:751375096
1231 1231 -, ctct dbSNP:58782258
1232 1232 c, t dbSNP:151105116
1233 1233 c, t dbSNP:202140498
1238 1238 c, g dbSNP:532682469
1239 1239 -, ct dbSNP:553004572
1251 1251 -, ctc dbSNP:563553918
1254 1254 -, ct dbSNP:747734953
1262 1262 a, t dbSNP:113866163
1285 1285 -, ct dbSNP:139623690
1301 1301 -, ctct dbSNP:565062214
1306 1306 -, tctc dbSNP:201974024
1318 1318 -, ct dbSNP:371978648
1337 1337 c, t dbSNP:547797939
1342 1342 c, t dbSNP:370068353
1347 1347 c, g dbSNP:536035144
1380 1380 c, t dbSNP:559803513
1381 1381 c, g dbSNP:530392108
1401 1401 -, ctct dbSNP:150380411
1403 1403 c, g dbSNP:373066608
1405 1405 a, c dbSNP:548868928
1431 1431 c, g dbSNP:12930821
1441 1441 c, g dbSNP:531016950
1448 1448 c, t dbSNP:552540257
1449 1449 c, t dbSNP:570503152
1469 1469 a, g dbSNP:191811468
1473 1473 a, g dbSNP:183630288
1485 1485 c, t dbSNP:376388970
1487 1487 c, t dbSNP:574116680
1555 1555 c, g dbSNP:185385414
1566 1566 c, t dbSNP:539869982
1573 1573 c, g dbSNP:575497717
1580 1580 a, g dbSNP:545958819
1581 1581 c, t dbSNP:564161180
1582 1582 a, g dbSNP:577377913
1636 1636 c, t dbSNP:768960345
1638 1638 a, c dbSNP:774311786
1648 1648 c, t dbSNP:11551263
1652 1652 c, t dbSNP:765434178
1730 1730 c, g dbSNP:753128270
1754 1754 g, t dbSNP:190241788
1756 1756 a, g dbSNP:182095431
1758 1758 g, t dbSNP:758660280
1793 1793 c, t dbSNP:144654181
1839 1839 c, t dbSNP:62041779
1861 1861 a, g dbSNP:540042603
1863 1863 c, g dbSNP:755679865
1881 1881 a, g dbSNP:552719952
1889 1889 c, t dbSNP:779638358
1894 1894 a, g dbSNP:748960478
1908 1908 c, t dbSNP:148180082
1916 1916 c, g dbSNP:375005880
1941 1941 a, g dbSNP:113980652
2034 2034 a, t dbSNP:778701096
2054 2054 a, g dbSNP:748012386
2108 2108 a, g, t dbSNP:528146538
2151 2151 c, t dbSNP:186728160
2163 2163 a, g dbSNP:773236980
2190 2190 a, g dbSNP:567676558
2227 2227 c, t dbSNP:11551264
2233 2233 -, ac dbSNP:776133409
2234 2234 c, t dbSNP:535429733

Target ORF information:

RefSeq Version NM_024598
Organism Homo sapiens (human)
Definition Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_024598

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGAGCGCGG CGCCCCTGGT GGGCTACAGC AGCAGCGGCT CCGAGGATGA GTCCGAGGAC 
GGGATGCGGA CCAGGCCGGG GGATGGGAGC CACCGTCGTG GCCAGAGCCC CCTTCCCAGG
CAGAGATTTC CAGTACCTGA CAGTGTGCTG AACATGTTCC CGGGCACCGA GGAGGGGCCT
GAAGATGACA GCACAAAACA CGGGGGACGG GTGCGCACCT TCCCCCACGA GCGAGGCAAC
TGGGCCACCC ACGTCTATGT ACCATATGAA GCCAAGGAGG AGTTCCTGGA TCTGCTTGAT
GTGTTGCTGC CCCATGCCCA GACATATGTC CCCCGGCTGG TAAGGATGAA GGTGTTCCAC
CTCAGCCTGT CCCAGAGTGT GGTTCTGCGC CACCACTGGA TCCTCCCCTT CGTGCAGGCT
CTGAAAGCCC GTATGACCTC CTTCCACAGA TTCTTCTTTA CTGCCAACCA GGTAAAGATT
TACACCAATC AAGAGAAAAC CAGGACCTTT ATTGGGCTTG AGGTCACTTC AGGGCATGCC
CAGTTCCTGG ACCTGGTTTC AGAGGTGGAC AGAGTCATGG AGGAATTCAA CCTCACCACT
TTCTACCAGG ATCCTTCTTT CCACCTCAGC CTGGCCTGGT GTGTGGGTGA TGCACGTCTC
CAGCTGGAGG GGCAGTGCCT GCAGGAACTA CAGGCAATCG TGGATGGGTT TGAAGATGCT
GAGGTGCTGC TGCGCGTGCA CACTGAGCAA GTCCGCTGCA AGTCTGGGAA CAAGTTCTTC
TCGATGCCTT TGAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu07663
Accession Version NM_001195302.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product U6 snRNA phosphodiesterase isoform 2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC363309.1, AK303121.1, AC012182.7 and DB364698.1. Summary: This gene encodes a protein with several conserved domains, however, its exact function is not known. Mutations in this gene are associated with poikiloderma with neutropenia (PN), which shows phenotypic overlap with Rothmund-Thomson syndrome (RTS) caused by mutations in the RECQL4 gene. It is believed that this gene product interacts with RECQL4 protein via SMAD4 proteins, explaining the partial clinical overlap between PN and RTS. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]. Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303121.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGAGCGCGG CGCCCCTGGT GGGCTACAGC AGCAGCGGCT CCGAGGATGA GTCCGAGGAC 
GGGATGCGGA CCAGGCCGGG GGATGGGAGC CACCGTCGTG GCCAGAGCCC CCTTCCCAGG
CAGAGATTTC CAGTACCTGA CAGTGTGCTG AACATGTTCC CGGGCACCGA GGAGGGGCCT
GAAGATGACA GCACAAAACA CGGGGGACGG GTGCGCACCT TCCCCCACGA GCGAGGCAAC
TGGGCCACCC ACGTCTATGT ACCATATGAA GCCAAGGAGG AGTTCCTGGA TCTGCTTGAT
GTGTTGCTGC CCCATGCCCA GACATATGTC CCCCGGCTGG TAAGGATGAA GGTGTTCCAC
CTCAGCCTGT CCCAGAGTGT GGTTCTGCGC CACCACTGGA TCCTCCCCTT CGTGCAGGCT
CTGAAAGCCC GTATGACCTC CTTCCACAGG ACCTTTATTG GGCTTGAGGT CACTTCAGGG
CATGCCCAGT TCCTGGACCT GGTTTCAGAG GTGGACAGAG TCATGGAGGA ATTCAACCTC
ACCACTTTCT ACCAGGATCC TTCTTTCCAC CTCAGCCTGG CCTGGTGTGT GGGTGATGCA
CGTCTCCAGC TGGAGGGGCA GTGCCTGCAG GAACTACAGG CAATCGTGGA TGGGTTTGAA
GATGCTGAGG TGCTGCTGCG CGTGCACACT GAGCAAGTCC GCTGCAAGTC TGGGAACAAG
TTCTTCTCGA TGCCTTTGAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001182231.1
CDS112..855
Misc Feature(1)103..105(+)
Misc Feature(2)241..849(+)
Exon (1)1..209
Gene:USB1
Gene Synonym:
Exon (2)210..376
Gene:USB1
Gene Synonym:
Exon (3)377..560
Gene:USB1
Gene Synonym:
Exon (4)561..666
Gene:USB1
Gene Synonym:
Exon (5)667..750
Gene:USB1
Gene Synonym:
Exon (6)751..2233
Gene:USB1
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, t dbSNP:539113150
41 41 a, g dbSNP:749839080
42 42 a, g dbSNP:557453429
49 49 g, t dbSNP:572460232
55 55 c, t dbSNP:539851929
59 59 g, t dbSNP:555381360
61 61 c, g dbSNP:73546949
63 63 c, t dbSNP:755207534
64 64 a, g dbSNP:544521629
66 66 g, t dbSNP:748260509
75 75 c, g dbSNP:758458788
78 78 a, g dbSNP:562526527
82 82 a, g dbSNP:778195515
83 83 a, c dbSNP:747515828
86 86 a, c, g dbSNP:201250414
92 92 c, g dbSNP:746174189
99 99 a, t dbSNP:768425672
100 100 g, t dbSNP:774106572
101 101 a, c, g dbSNP:543492919
104 104 a, g dbSNP:370555892
107 107 g, t dbSNP:773091216
112 112 a, g dbSNP:760648279
114 114 c, g dbSNP:546288690
117 117 c, t dbSNP:753636337
124 124 -, c dbSNP:781151070
124 124 c, t dbSNP:759290159
126 126 c, t dbSNP:765469310
131 131 c, t dbSNP:373657742
134 134 a, g dbSNP:564572047
141 141 c, g dbSNP:777899627
142 142 a, t dbSNP:752060249
149 149 a, g dbSNP:757792250
153 153 a, c dbSNP:3743559
165 165 c, g dbSNP:746264362
167 167 a, g dbSNP:770232543
172 172 a, g dbSNP:778756392
172 172 -, g dbSNP:747958554
178 178 c, t dbSNP:747805446
179 179 c, g dbSNP:771539759
185 185 c, g dbSNP:772792606
189 189 c, g dbSNP:375706004
191 191 c, g dbSNP:770934785
201 201 a, c dbSNP:149411375
208 208 c, g dbSNP:759378341
211 211 a, g dbSNP:769630452
213 213 c, g, t dbSNP:144716560
215 215 a, c dbSNP:762585706
217 217 a, g dbSNP:138405924
219 219 a, c dbSNP:774385750
220 220 c, t dbSNP:762001177
222 222 c, t dbSNP:767672950
226 226 c, t dbSNP:751037751
235 235 a, t dbSNP:756702350
240 240 c, t dbSNP:766926835
245 245 -, t dbSNP:774728454
259 259 c, t dbSNP:754262096
260 260 c, t dbSNP:755250981
273 273 a, g dbSNP:143792185
275 275 a, g dbSNP:746613694
276 276 c, g, t dbSNP:756831751
280 280 a, g dbSNP:745321157
281 281 a, c dbSNP:769724028
285 285 -, gg dbSNP:759761915
290 290 -, c dbSNP:786205051
299 299 a, t dbSNP:775249170
305 305 a, c dbSNP:749129393
312 312 c, t dbSNP:548754947
313 313 a, g dbSNP:774672635
316 316 a, g dbSNP:762121009
317 317 a, g dbSNP:767791131
319 319 a, c, t dbSNP:531886363
320 320 a, g dbSNP:767016894
324 324 a, g dbSNP:369515724
325 325 c, t dbSNP:567360825
326 326 a, c, g dbSNP:372917091
329 329 c, t dbSNP:751288061
335 335 c, t dbSNP:763524876
337 337 a, c dbSNP:780752842
339 339 c, t dbSNP:549783755
341 341 a, c dbSNP:755570589
342 342 g, t dbSNP:371393773
343 343 a, c dbSNP:374559185
354 354 a, g dbSNP:137853973
355 355 a, g dbSNP:778559247
363 363 c, t dbSNP:147246452
364 364 a, g dbSNP:764580227
366 366 c, t dbSNP:375733174
369 369 c, t dbSNP:199820065
370 370 a, g dbSNP:571564926
374 374 c, g dbSNP:770881616
378 378 c, t dbSNP:771096742
387 387 a, g dbSNP:776583555
401 401 a, t dbSNP:759598117
414 414 a, g dbSNP:770239330
423 423 c, t dbSNP:140760824
428 428 c, t dbSNP:763359090
436 436 -, t dbSNP:754368529
437 437 a, g dbSNP:764413085
438 438 c, t dbSNP:369321909
446 446 a, g dbSNP:142683238
447 447 a, g, t dbSNP:765998448
455 455 a, g dbSNP:35025252
461 461 a, g dbSNP:778763495
469 469 c, t dbSNP:752648429
474 474 c, t dbSNP:758332726
478 478 c, g, t dbSNP:777694507
488 488 a, g dbSNP:757421099
492 492 a, g dbSNP:781440519
498 498 c, g dbSNP:542658449
499 499 c, t dbSNP:769898628
500 500 a, g dbSNP:199886884
513 513 c, g dbSNP:776019365
522 522 c, t dbSNP:749793676
523 523 a, g dbSNP:769067404
534 534 c, g dbSNP:774712588
536 536 a, g dbSNP:762197073
541 541 c, t dbSNP:774644058
542 542 a, g dbSNP:367780234
542 542 -, g dbSNP:762227910
558 558 c, g dbSNP:776322680
562 562 a, t dbSNP:779896567
569 569 c, t dbSNP:149725439
577 577 c, g dbSNP:768341871
590 590 a, g dbSNP:374426055
592 592 c, t dbSNP:762828282
597 597 c, g dbSNP:761161720
621 621 a, g dbSNP:772079518
625 625 a, g dbSNP:772999682
628 628 a, g dbSNP:760502631
640 640 a, g dbSNP:766156595
641 641 a, c dbSNP:754116399
647 647 a, g dbSNP:377411772
649 649 c, t dbSNP:765307761
650 650 g, t dbSNP:555309420
655 655 a, g dbSNP:758357702
656 656 c, g dbSNP:764715431
663 663 c, t dbSNP:764176381
681 681 c, t dbSNP:371949436
696 696 a, c, g dbSNP:750851175
698 698 a, g dbSNP:146685901
703 703 a, g dbSNP:778666156
712 712 c, t dbSNP:554516072
713 713 a, g dbSNP:757982322
724 724 c, g dbSNP:777276672
728 728 a, g dbSNP:746447180
750 750 a, g dbSNP:770883144
754 754 a, g dbSNP:769032208
756 756 c, t dbSNP:774669193
757 757 a, g dbSNP:762033139
769 769 a, g dbSNP:772272501
773 773 a, g dbSNP:773826400
780 780 c, g dbSNP:761098113
782 782 c, t dbSNP:766841291
786 786 a, g dbSNP:190283472
790 790 c, t dbSNP:182418203
791 791 a, g dbSNP:140289507
792 792 c, t dbSNP:763780991
793 793 a, g dbSNP:751177454
802 802 a, g dbSNP:756831672
805 805 c, g dbSNP:16959641
811 811 a, c, t dbSNP:146419360
812 812 a, g dbSNP:780003077
828 828 c, g dbSNP:149041481
839 839 c, t dbSNP:368558020
840 840 c, g dbSNP:779320272
841 841 a, g dbSNP:143382202
844 844 c, t dbSNP:772360295
845 845 c, t dbSNP:773169264
852 852 a, g dbSNP:760767630
857 857 c, t dbSNP:771359873
859 859 a, c dbSNP:777169639
869 869 c, t dbSNP:759932035
879 879 c, g dbSNP:765579501
895 895 a, g dbSNP:774051099
957 957 a, c dbSNP:772110530
1003 1003 c, g dbSNP:772987427
1021 1021 -, tctc dbSNP:370473790
1033 1033 -, tt dbSNP:759921215
1049 1049 -, ttct dbSNP:372237467
1060 1060 -, ctc dbSNP:752765084
1063 1063 -, ctct dbSNP:200950416
1065 1065 -, ct dbSNP:762941380
1076 1076 -, ct dbSNP:751796506
1077 1077 -, tctg dbSNP:142769438
1080 1080 -, gtct dbSNP:375560719
1081 1081 g, t dbSNP:35071272
1094 1094 -, ct dbSNP:549920112
1106 1106 c, t dbSNP:575397821
1108 1108 c, t dbSNP:113112209
1109 1109 -, tctc dbSNP:547553387
1116 1116 -, tcctc dbSNP:527401961
1139 1139 -, tctc dbSNP:547883874
1151 1151 c, g, t dbSNP:113876388
1156 1156 -, tg dbSNP:754995890
1157 1157 c, g dbSNP:549064355
1159 1159 c, t dbSNP:565377163
1161 1161 -, ctcctcccctc dbSNP:747135009
1171 1171 -, ct, ctct dbSNP:112874007
1171 1171 -, c dbSNP:369788319
1172 1172 -, ctct dbSNP:72112945
1174 1174 -, ctcttcctctc dbSNP:749283271
1174 1174 -, ctct dbSNP:386384807
1176 1176 -, cctc dbSNP:751375096
1177 1177 -, ctct dbSNP:58782258
1178 1178 c, t dbSNP:151105116
1179 1179 c, t dbSNP:202140498
1184 1184 c, g dbSNP:532682469
1185 1185 -, ct dbSNP:553004572
1197 1197 -, ctc dbSNP:563553918
1200 1200 -, ct dbSNP:747734953
1208 1208 a, t dbSNP:113866163
1231 1231 -, ct dbSNP:139623690
1247 1247 -, ctct dbSNP:565062214
1252 1252 -, tctc dbSNP:201974024
1264 1264 -, ct dbSNP:371978648
1283 1283 c, t dbSNP:547797939
1288 1288 c, t dbSNP:370068353
1293 1293 c, g dbSNP:536035144
1326 1326 c, t dbSNP:559803513
1327 1327 c, g dbSNP:530392108
1347 1347 -, ctct dbSNP:150380411
1349 1349 c, g dbSNP:373066608
1351 1351 a, c dbSNP:548868928
1377 1377 c, g dbSNP:12930821
1387 1387 c, g dbSNP:531016950
1394 1394 c, t dbSNP:552540257
1395 1395 c, t dbSNP:570503152
1415 1415 a, g dbSNP:191811468
1419 1419 a, g dbSNP:183630288
1431 1431 c, t dbSNP:376388970
1433 1433 c, t dbSNP:574116680
1501 1501 c, g dbSNP:185385414
1512 1512 c, t dbSNP:539869982
1519 1519 c, g dbSNP:575497717
1526 1526 a, g dbSNP:545958819
1527 1527 c, t dbSNP:564161180
1528 1528 a, g dbSNP:577377913
1582 1582 c, t dbSNP:768960345
1584 1584 a, c dbSNP:774311786
1594 1594 c, t dbSNP:11551263
1598 1598 c, t dbSNP:765434178
1676 1676 c, g dbSNP:753128270
1700 1700 g, t dbSNP:190241788
1702 1702 a, g dbSNP:182095431
1704 1704 g, t dbSNP:758660280
1739 1739 c, t dbSNP:144654181
1785 1785 c, t dbSNP:62041779
1807 1807 a, g dbSNP:540042603
1809 1809 c, g dbSNP:755679865
1827 1827 a, g dbSNP:552719952
1835 1835 c, t dbSNP:779638358
1840 1840 a, g dbSNP:748960478
1854 1854 c, t dbSNP:148180082
1862 1862 c, g dbSNP:375005880
1887 1887 a, g dbSNP:113980652
1980 1980 a, t dbSNP:778701096
2000 2000 a, g dbSNP:748012386
2054 2054 a, g, t dbSNP:528146538
2097 2097 c, t dbSNP:186728160
2109 2109 a, g dbSNP:773236980
2136 2136 a, g dbSNP:567676558
2173 2173 c, t dbSNP:11551264
2179 2179 -, ac dbSNP:776133409
2180 2180 c, t dbSNP:535429733

Target ORF information:

RefSeq Version NM_001195302
Organism Homo sapiens (human)
Definition Homo sapiens U6 snRNA biogenesis 1 (USB1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001195302

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGAGCGCGG CGCCCCTGGT GGGCTACAGC AGCAGCGGCT CCGAGGATGA GTCCGAGGAC 
GGGATGCGGA CCAGGCCGGG GGATGGGAGC CACCGTCGTG GCCAGAGCCC CCTTCCCAGG
CAGAGATTTC CAGTACCTGA CAGTGTGCTG AACATGTTCC CGGGCACCGA GGAGGGGCCT
GAAGATGACA GCACAAAACA CGGGGGACGG GTGCGCACCT TCCCCCACGA GCGAGGCAAC
TGGGCCACCC ACGTCTATGT ACCATATGAA GCCAAGGAGG AGTTCCTGGA TCTGCTTGAT
GTGTTGCTGC CCCATGCCCA GACATATGTC CCCCGGCTGG TAAGGATGAA GGTGTTCCAC
CTCAGCCTGT CCCAGAGTGT GGTTCTGCGC CACCACTGGA TCCTCCCCTT CGTGCAGGCT
CTGAAAGCCC GTATGACCTC CTTCCACAGG ACCTTTATTG GGCTTGAGGT CACTTCAGGG
CATGCCCAGT TCCTGGACCT GGTTTCAGAG GTGGACAGAG TCATGGAGGA ATTCAACCTC
ACCACTTTCT ACCAGGATCC TTCTTTCCAC CTCAGCCTGG CCTGGTGTGT GGGTGATGCA
CGTCTCCAGC TGGAGGGGCA GTGCCTGCAG GAACTACAGG CAATCGTGGA TGGGTTTGAA
GATGCTGAGG TGCTGCTGCG CGTGCACACT GAGCAAGTCC GCTGCAAGTC TGGGAACAAG
TTCTTCTCGA TGCCTTTGAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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