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CUBN cDNA ORF clone, Homo sapiens (human)

Gene Symbol CUBN
Entrez Gene ID 8029
Full Name cubilin (intrinsic factor-cobalamin receptor)
Synonyms IFCR, MGA1, gp280
General protein information
Preferred Names
cubilin
Names
cubilin
460 kDa receptor
cubilin precursor variant 1
cubilin precursor variant 2
intestinal intrinsic factor receptor
intrinsic factor-vitamin B12 receptor
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10p12.31

Summary Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. The role of receptor is supported by the presence of 27 CUB domains. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

602997

Disorder Html: Megaloblastic anemia-1, Finnish type, 261100 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001081 NP_001072 cubilin precursor
XM_011519708 XP_011518010 cubilin isoform X1
XM_011519709 XP_011518011 cubilin isoform X2
XM_011519710 XP_011518012 cubilin isoform X3
XM_011519711 XP_011518013 cubilin isoform X4



Homo sapiens (human) CUBN NP_001072.2
Canis lupus familiaris (dog) CUBN NP_001003148.1
Bos taurus (cattle) CUBN NP_001179504.1
Mus musculus (house mouse) Cubn NP_001074553.1
Rattus norvegicus (Norway rat) Cubn NP_445784.1
Gallus gallus (chicken) CUBN XP_001235156.3
Danio rerio (zebrafish) cubn XP_002666724.2
Drosophila melanogaster (fruit fly) CG32702 NP_727348.2
Caenorhabditis elegans ZC116.3 NP_506157.3
Xenopus (Silurana) tropicalis (western clawed frog) cubn XP_002939051.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CUBN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CUBN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu24823 NM_001081 Homo sapiens cubilin (intrinsic factor-cobalamin receptor) (CUBN), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD Quote Price
OHu61599 XM_011519708 PREDICTED: Homo sapiens cubilin (intrinsic factor-cobalamin receptor) (CUBN), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1511.30
$2159.00
OHu61600 XM_011519709 PREDICTED: Homo sapiens cubilin (intrinsic factor-cobalamin receptor) (CUBN), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu61601 XM_011519710 PREDICTED: Homo sapiens cubilin (intrinsic factor-cobalamin receptor) (CUBN), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu61602 XM_011519711 PREDICTED: Homo sapiens cubilin (intrinsic factor-cobalamin receptor) (CUBN), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu24823
Clone ID Related Accession (Same CDS sequence) NM_001081
Accession Version NM_001081.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 10872bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product cubilin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP271329.1, AF034611.1, AC067747.7, BP270790.1, AL731551.9 and AL365215.23. This sequence is a reference standard in the RefSeqGene project. On Feb 23, 2007 this sequence version replaced gi:21536291. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF034611.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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ATGATGAACA TGTCTTTACC TTTTCTTTGG AGTTTGCTTA CCTTATTAAT ATTTGCTGAA 
GTAAATGGCG AAGCTGGAGA ACTTGAGCTG CAGAGACAAA AAAGAAGCAT CAATCTCCAA
CAGCCTCGAA TGGCTACAGA GAGAGGAAAT TTGGTGTTTC TTACGGGGTC TGCTCAAAAC
ATTGAGTTTA GAACCGGATC CCTGGGAAAA ATTAAATTAA ATGATGAAGA TCTCAGTGAG
TGTTTACATC AGATCCAGAA AAACAAAGAA GATATTATAG AGTTAAAAGG GAGTGCAATT
GGTCTGCCTC AAAATATATC TAGTCAAATC TATCAGCTTA ATTCCAAGCT GGTGGATCTT
GAGAGAAAAT TCCAAGGCTT GCAGCAGACT GTTGACAAAA AGGTTTGCAG CAGCAATCCT
TGCCAGAATG GTGGAACCTG CCTCAATCTG CATGATTCCT TTTTTTGTAT CTGTCCCCCA
CAGTGGAAGG GTCCTCTCTG CTCAGCTGAT GTTAACGAAT GTGAGATTTA CTCAGGAACA
CCCTTGAGCT GCCAGAATGG AGGCACATGT GTTAATACAA TGGGAAGTTA CAGTTGTCAC
TGCCCACCTG AGACGTACGG ACCCCAGTGT GCATCCAAAT ATGACGACTG TGAAGGGGGT
TCTGTGGCAC GCTGTGTCCA TGGCATCTGT GAGGATTTAA TGCGAGAGCA AGCTGGAGAG
CCCAAGTACA GCTGCGTCTG TGATGCTGGG TGGATGTTTT CACCCAACAG CCCTGCCTGC
ACGCTGGACA GAGACGAGTG CAGCTTCCAG CCCGGGCCTT GCTCCACACT TGTGCAGTGT
TTCAACACTC AAGGCTCTTT CTACTGTGGG GCCTGTCCAA CAGGCTGGCA AGGCAATGGA
TATATTTGCG AAGATATCAA TGAATGTGAG ATAAATAACG GCGGCTGTTC TGTGGCTCCA
CCCGTTGAGT GTGTGAATAC ACCTGGGTCT TCCCACTGCC AGGCCTGTCC ACCAGGGTAC
CAGGGTGACG GAAGAGTGTG CACACTCACA GACATCTGCT CAGTCAGTAA TGGAGGCTGC
CACCCAGATG CCTCATGCTC CTCAACTCTA GGTTCCTTAC CTCTCTGCAC GTGTCTCCCG
GGTTATACTG GAAATGGTTA TGGGCCAAAT GGATGTGTGC AGCTCAGTAA TATTTGCCTA
AGTCACCCCT GTCTAAATGG ACAATGCATC GACACTGTCT CTGGTTATTT TTGTAAGTGT
GACTCAGGTT GGACAGGTGT CAACTGTACA GAAAACATCA ATGAGTGTTT GAGCAACCCC
TGTTTGAATG GAGGAACTTG TGTTGATGGC GTTGATTCTT TCAGTTGTGA ATGCACACGT
CTCTGGACTG GAGCTCTCTG TCAGGTTCCT CAGCAAGTTT GTGGAGAGTC CCTCTCAGGA
ATAAATGGAA GCTTCAGCTA CAGGAGCCCG GATGTTGGTT ATGTTCATGA TGTTAACTGC
TTCTGGGTTA TCAAAACTGA AATGGGAAAG GTCCTGCGTA TCACTTTCAC TTTTTTCCGG
TTAGAATCCA TGGACAACTG TCCACACGAG TTTCTTCAGG TTTATGATGG AGATTCCTCT
TCTGCTTTTC AACTTGGAAG ATTTTGTGGC TCCAGCCTCC CTCATGAACT CCTCAGCAGT
GACAATGCTC TCTATTTTCA TCTCTATTCT GAACATTTAA GAAATGGGAG AGGCTTTACA
GTAAGATGGG AAACACAGCA ACCAGAGTGT GGAGGTATCC TGACTGGTCC TTACGGTTCT
ATTAAGTCTC CGGGGTATCC TGGAAACTAT CCCCCAGGAA GAGATTGTGT CTGGATTGTT
GTAACTAGTC CTGACCTCCT GGTAACATTT ACTTTTGGGA CCTTGAGCCT CGAGCACCAT
GATGACTGCA ACAAAGATTA CCTTGAGATT CGAGATGGTC CTTTGTATCA GGACCCCCTT
CTTGGGAAGT TCTGCACCAC TTTCTCTGTC CCACCGCTCC AGACTACTGG CCCCTTTGCC
AGAATTCACT TCCATTCAGA CTCCCAGATT AGTGACCAAG GCTTCCATAT CACCTACTTA
ACATCACCTT CGGATCTGCG TTGTGGTGGG AACTACACGG ACCCAGAGGG TGAACTCTTC
TTGCCTGAGT TGTCTGGGCC TTTCACTCAC ACCAGGCAAT GCGTCTATAT GATGAAGCAG
CCCCAGGGAG AACAAATACA AATCAACTTC ACCCACGTGG AGCTGCAATG CCAGAGTGAC
AGTTCTCAGA ATTACATTGA GGTTCGAGAT GGTGAAACCT TACTTGGAAA AGTCTGTGGC
AACGGAACCA TCTCTCACAT TAAATCCATT ACTAATAGTG TCTGGATCAG GTTTAAAATA
GATGCTTCTG TTGAAAAAGC TAGTTTCAGA GCTGTTTATC AAGTCGCTTG CGGGGATGAA
TTAACTGGAG AAGGGGTCAT TCGCTCGCCT TTTTTTCCTA ACGTGTATCC TGGAGAAAGA
ACCTGTAGGT GGACCATCCA CCAGCCCCAA AGCCAAGTCA TTCTCCTCAA CTTCACTGTC
TTTGAAATTG GAAGTTCTGC CCACTGTGAA ACAGATTATG TTGAGATTGG TAGCAGTTCC
ATTTTGGGTT CTCCTGAAAA TAAAAAGTAT TGCGGTACAG ACATACCTTC ATTTATAACA
TCTGTGTACA ATTTTCTTTA TGTCACATTC GTGAAAAGTT CTTCTACTGA AAACCATGGT
TTCATGGCTA AGTTCAGTGC TGAGGATTTG GCATGTGGAG AAATTCTTAC AGAATCAACA
GGGACCATTC AAAGTCCTGG CCATCCAAAT GTCTACCCCC ACGGTATCAA CTGTACTTGG
CATATATTAG TCCAACCTAA TCACCTGATT CATTTAATGT TCGAAACATT TCATCTGGAG
TTTCATTACA ATTGCACAAA CGACTACTTG GAAGTTTATG ACACCGACTC TGAGACATCC
CTTGGAAGAT ACTGTGGAAA GTCGATCCCG CCATCTCTCA CAAGCAGTGG TAACTCATTG
ATGCTGGTGT TTGTGACTGA CTCCGACCTC GCTTATGAAG GCTTCTTAAT AAACTATGAA
GCAATCAGTG CAGCAACAGC ATGTTTGCAA GACTACACAG ATGATTTGGG GACATTCACT
TCTCCAAACT TCCCCAATAA TTATCCCAAC AACTGGGAAT GCATTTATCG GATCACAGTG
AGAACTGGCC AACTGATTGC AGTGCACTTC ACAAACTTCT CCTTGGAGGA AGCCATTGGA
AACTATTATA CAGATTTTCT GGAAATCAGA GATGGAGGCT ATGAAAAATC ACCATTGCTG
GGAATATTCT ATGGCTCAAA TCTACCCCCA ACAATCATCT CTCATAGTAA CAAACTATGG
TTAAAATTTA AGAGTGACCA AATAGACACA AGGTCTGGAT TCTCAGCTTA CTGGGATGGG
TCATCAACAG GTTGCGGGGG TAATCTCACC ACTTCAAGCG GCACGTTCAT ATCTCCCAAC
TACCCGATGC CCTATTACCA CAGCTCTGAA TGCTACTGGT GGTTGAAATC TAGCCACGGC
AGCGCATTTG AACTGGAATT CAAAGACTTT CACTTGGAGC ATCATCCAAA CTGCACTTTA
GATTACCTGG CTGTATATGA TGGCCCAAGT AGCAACTCTC ATCTGCTAAC TCAGCTTTGT
GGGGATGAGA AACCCCCTCT TATTCGTTCT AGTGGAGACA GCATGTTTAT AAAACTGAGG
ACAGATGAAG GTCAGCAAGG ACGTGGCTTC AAGGCTGAAT ACCGGCAGAC ATGTGAGAAT
GTGGTAATAG TCAATCAAAC CTATGGCATC TTAGAGAGTA TAGGGTATCC GAATCCTTAT
TCTGAAAATC AGCATTGCAA CTGGACCATC CGGGCAACAA CAGGCAACAC TGTGAACTAC
ACATTTTTAG CATTTGACTT GGAACATCAC ATAAACTGCT CCACAGATTA TTTAGAGCTC
TATGATGGAC CACGGCAGAT GGGACGCTAC TGTGGAGTAG ACCTGCCCCC TCCAGGGAGT
ACTACAAGCT CCAAGCTTCA AGTGCTGCTC CTTACAGATG GGGTTGGCCG CCGTGAGAAA
GGATTTCAGA TGCAGTGGTT TGTTTACGGT TGTGGTGGAG AGCTGTCTGG GGCCACAGGC
TCCTTCAGCA GCCCCGGGTT CCCCAACAGG TATCCACCAA ACAAGGAGTG TATCTGGTAC
ATTAGGACGG ACCCCGGGAG TAGCATTCAG CTCACCATCC ATGACTTCGA TGTGGAGTAT
CATTCAAGGT GCAACTTTGA TGTCTTGGAG ATCTATGGAG GCCCCGATTT CCACTCTCCC
AGAATAGCCC AACTGTGTAC CCAGAGATCA CCTGAGAACC CCATGCAGGT CTCCAGCACT
GGAAATGAGC TAGCAATTCG ATTCAAGACC GACTTGTCCA TAAATGGGAG AGGCTTCAAT
GCGTCATGGC AAGCAGTCAC TGGAGGTTGT GGTGGGATTT TCCAGGCTCC CAGTGGAGAG
ATTCATTCTC CAAATTACCC CAGTCCTTAT AGGAGCAACA CAGACTGTTC TTGGGTCATT
CGGGTTGACA GAAATCATCG TGTTCTCTTG AACTTCACTG ACTTTGATCT TGAACCACAA
GACTCTTGTA TTATGGCATA CGATGGCTTA AGCTCCACAA TGTCCCGCCT TGCCAGGACG
TGTGGAAGGG AGCAGCTGGC TAACCCCATC GTCTCCTCAG GAAACAGCCT CTTCTTGAGA
TTTCAGTCTG GCCCTTCCAG ACAGAACAGA GGCTTCCGAG CTCAATTCAG GCAAGCCTGC
GGAGGCCACA TCCTCACCAG CTCATTTGAT ACTGTTTCCT CTCCACGGTT CCCTGCCAAT
TATCCAAACA ATCAGAACTG CAGCTGGATC ATTCAAGCGC AACCTCCATT AAATCATATC
ACCCTCTCTT TTACCCACTT TGAACTTGAA AGAAGCACAA CGTGTGCACG TGACTTTGTA
GAAATTTTGG ATGGCGGCCA CGAAGACGCG CCCCTCCGAG GCCGTTACTG TGGCACCGAC
ATGCCCCATC CTATCACATC CTTCAGCAGC GCCCTGACGC TGAGATTCGT CTCTGATTCT
AGCATCAGTG CTGGGGGTTT CCACACCACG GTCACCGCAT CAGTGTCGGC TTGTGGTGGA
ACGTTCTACA TGGCTGAAGG CATCTTCAAC AGCCCTGGCT ACCCAGACAT TTATCCCCCT
AATGTGGAAT GTGTCTGGAA CATCGTCAGT TCCCCTGGCA ACCGGCTCCA GCTGTCTTTT
ATATCTTTCC AGTTGGAAGA CTCTCAGGAC TGCAGCAGAG ATTTTGTGGA GATCCGTGAA
GGAAATGCCA CGGGTCACTT GGTGGGACGA TACTGTGGAA ACTCCTTCCC TCTCAATTAT
TCTTCCATCG TTGGACATAC CCTGTGGGTC AGATTTATCT CAGATGGTTC TGGCAGCGGC
ACGGGCTTCC AGGCCACATT TATGAAGATA TTTGGCAATG ATAATATTGT GGGAACTCAT
GGGAAAGTCG CCTCTCCTTT CTGGCCTGAA AACTACCCAC ATAACTCCAA TTACCAATGG
ACAGTAAATG TGAATGCATC TCACGTTGTC CATGGTAGAA TCTTGGAGAT GGACATAGAA
GAAATACAAA ACTGCTATTA TGACAAATTA AGGATCTATG ATGGGCCTAG CATTCACGCC
CGCCTAATTG GAGCTTACTG TGGTACCCAG ACTGAATCTT TCAGCTCCAC TGGAAATTCT
TTGACATTTC ATTTTTACTC CGACTCTTCA ATCTCAGGGA AGGGATTCCT TCTGGAGTGG
TTTGCAGTGG ATGCACCTGA TGGTGTTTTA CCTACCATTG CTCCAGGTGC TTGTGGTGGC
TTCCTGAGGA CGGGAGATGC ACCCGTGTTT CTCTTCTCCC CGGGCTGGCC TGACAGTTAC
AGTAATAGAG TGGACTGTAC GTGGCTCATC CAGGCTCCCG ACTCTACCGT GGAACTCAAC
ATTCTTTCCC TGGACATTGA ATCTCACCGA ACGTGTGCCT ATGATAGCCT TGTGATACGA
GATGGAGATA ATAACTTGGC CCAGCAGCTA GCAGTTCTCT GTGGCAGAGA GATCCCTGGG
CCCATCCGGT CTACTGGAGA GTACATGTTC ATCCGCTTCA CCTCGGACTC CAGTGTAACC
AGGGCAGGCT TCAATGCATC CTTTCACAAG AGCTGCGGTG GATATTTGCA TGCAGACAGA
GGGATCATCA CGTCCCCCAA GTATCCAGAG ACTTACCCAT CCAACCTCAA CTGTTCTTGG
CACGTCCTGG TCCAAAGTGG CCTGACCATT GCTGTCCATT TTGAACAGCC TTTCCAGATT
CCAAATGGAG ATTCTTCTTG CAACCAGGGG GATTACTTGG TGCTAAGAAA TGGTCCTGAT
ATCTGTTCTC CACCCTTGGG ACCCCCTGGA GGAAATGGTC ATTTTTGTGG CAGTCATGCT
TCATCAACTC TGTTCACCTC GGATAATCAA ATGTTTGTTC AGTTTATTTC TGATCACAGT
AATGAAGGGC AAGGATTTAA AATCAAATAT GAGGCAAAGA GTTTAGCCTG TGGGGGCAAC
GTCTACATCC ATGATGCTGA TTCTGCTGGG TATGTGACCT CCCCCAACCA CCCTCATAAT
TATCCCCCGC ACGCTGATTG CATTTGGATC TTAGCGGCTC CACCGGAAAC ACGCATACAG
CTGCAATTTG AAGATCGATT CGATATTGAA GTAACACCCA ACTGTACTTC CAACTACCTT
GAGTTGCGGG ATGGAGTGGA TTCGGATGCA CCAATACTTT CCAAATTTTG TGGGACATCT
TTGCCCAGCA GTCAGTGGTC CTCAGGAGAG GTTATGTATT TGAGATTTCG ATCTGACAAC
AGCCCCACAC ATGTGGGATT CAAGGCCAAG TATTCTATAG CTCAGTGTGG GGGAAGAGTA
CCAGGGCAAA GTGGTGTTGT TGAAAGCATT GGACATCCAA CACTTCCATA CAGAGACAAC
TTATTCTGTG AGTGGCATCT CCAGGGGCTC TCTGGACACT ATCTCACCAT CTCTTTTGAA
GACTTTAACC TTCAGAATTC TTCTGGCTGT GAAAAAGACT TCGTGGAGAT CTGGGACAAT
CATACCTCTG GAAACATCTT GGGCAGATAC TGTGGAAACA CCATTCCTGA CAGCATAGAC
ACTTCTAGCA ATACTGCTGT GGTCAGGTTT GTCACAGACG GCTCTGTGAC TGCCTCAGGA
TTCAGACTGC GATTTGAATC CAGTATGGAA GAGTGTGGTG GGGATCTTCA GGGCTCTATT
GGAACATTTA CTTCTCCCAA CTACCCGAAC CCAAATCCTC ATGGCCGGAT CTGCGAGTGG
AGAATCACTG CCCCGGAGGG AAGGCGGATC ACCCTAATGT TTAACAACCT GAGGCTGGCC
ACGCATCCGT CCTGCAACAA TGAGCATGTG ATAGTATTCA ATGGCATTAG AAGTAACTCA
CCCCAGCTAG AGAAACTGTG TAGTAGTGTG AATGTAAGCA ATGAGATTAA ATCTTCAGGA
AACACAATGA AAGTCATTTT TTTCACGGAT GGATCCAGGC CATATGGCGG CTTCACTGCT
TCCTATACCT CCAGTGAAGA TGCAGTGTGT GGTGGGTCTC TTCCAAATAC TCCTGAAGGA
AACTTTACTT CTCCTGGCTA TGACGGAGTC AGGAATTACT CAAGAAACCT GAACTGCGAA
TGGACTCTCA GCAATCCAAA TCAGGGAAAT TCATCCATTT CCATTCACTT TGAAGATTTT
TACCTAGAAA GTCACCAAGA CTGTCAATTT GATGTCCTCG AGTTTCGAGT GGGTGATGCT
GATGGGCCCC TGATGTGGAG ACTTTGTGGT CCTTCAAAGC CTACATTGCC ATTGGTTATA
CCTTATTCTC AGGTATGGAT TCACTTTGTC ACCAACGAAC GTGTAGAACA CATTGGATTC
CATGCAAAGT ATTCCTTTAC AGATTGTGGC GGAATACAGA TAGGTGACAG TGGAGTGATC
ACAAGCCCCA ACTATCCAAA TGCTTATGAC AGCCTGACCC ACTGCTCTTC GCTGTTGGAG
GCCCCACAAG GGCACACCAT CACTCTCACA TTTAGTGACT TTGATATTGA ACCCCATACA
ACTTGTGCTT GGGACTCTGT CACTGTCAGG AATGGTGGGT CCCCTGAATC ACCCATCATA
GGACAATACT GTGGAAATTC AAACCCCAGG ACAATACAGT CAGGTTCCAA TCAGCTGGTC
GTGACTTTTA ACTCAGACCA TTCATTGCAA GGTGGTGGAT TTTATGCTAC GTGGAACACA
CAAACTTTAG GTTGTGGTGG AATATTTCAT TCTGATAATG GTACAATCAG ATCCCCTCAC
TGGCCTCAGA ATTTTCCCGA AAACAGCAGA TGTTCCTGGA CGGCCATTAC TCACAAAAGT
AAACACTTGG AGATCAGCTT TGACAACAAC TTCCTAATCC CCAGCGGTGA TGGACAATGT
CAGAATAGCT TCGTGAAGGT GTGGGCAGGA ACTGAGGAGG TGGACAAAGC CCTGCTAGCC
ACTGGCTGTG GGAACGTGGC TCCGGGTCCC GTTATCACAC CAAGTAACAC ATTCACTGCC
GTCTTCCAGT CTCAGGAGGC ACCAGCTCAG GGCTTCTCCG CGTCCTTTGT TAGCCGATGT
GGAAGTAATT TCACTGGCCC TTCAGGTTAC ATCATTTCTC CAAATTACCC AAAACAATAT
GACAACAACA TGAATTGCAC CTATGTCATA GAGGCTAATC CTCTGTCAGT GGTCCTCTTG
ACTTTTGTGT CCTTCCACTT AGAAGCTCGT TCCGCTGTGA CGGGAAGCTG TGTCAACGAT
GGCGTGCACA TTATCAGAGG TTACAGCGTC ATGTCCACCC CATTTGCTAC TGTGTGTGGG
GATGAGATGC CAGCTCCCCT CACCATCGCT GGGCCGGTTC TGCTTAACTT CTACTCCAAC
GAGCAAATCA CAGACTTCGG ATTCAAGTTT TCCTATAGGA TAATCTCCTG TGGTGGTGTG
TTCAATTTCT CTTCTGGAAT CATCACAAGT CCTGCCTATT CATACGCAGA CTACCCAAAT
GATATGCACT GTCTGTATAC CATCACCGTT AGTGACGACA AGGTGATCGA GCTCAAGTTC
AGTGATTTTG ATGTGGTTCC CTCCACCTCC TGCTCCCATG ACTACCTGGC AATTTACGAT
GGTGCCAATA CCAGCGATCC CCTTCTTGGC AAATTCTGCG GTTCCAAGCG CCCACCAAAT
GTGAAGAGCA GCAATAATAG TATGCTCCTG GTGTTCAAGA CAGATTCATT TCAGACAGCA
AAAGGCTGGA AGATGTCTTT CCGGCAGACA TTGGGGCCTC AGCAAGGATG TGGTGGTTAT
CTGACAGGCT CGAATAATAC CTTTGCCTCT CCTGATTCTG ATTCGAATGG AATGTATGAC
AAGAATTTAA ACTGTGTATG GATCATAATT GCACCTGTAA ACAAAGTAAT TCACCTCACC
TTCAATACAT TTGCTCTGGA GGCAGCAAGT ACTAGGCAAA GATGCCTTTA TGATTATGTA
AAGTTATATG ATGGGGATAG TGAAAATGCG AACTTGGCTG GAACGTTTTG TGGTTCCACA
GTACCTGCTC CTTTTATCTC TTCTGGTAAC TTCCTTACGG TTCAATTCAT CAGTGACTTA
ACATTAGAGA GGGAAGGATT TAATGCTACA TACACCATCA TGGACATGCC TTGTGGTGGA
ACATACAATG CAACTTGGAC CCCACAAAAT ATTTCATCAC CCAATTCATC AGACCCAGAT
GTCCCATTTT CCATCTGTAC TTGGGTCATT GATTCCCCTC CGCATCAGCA GGTCAAGATA
ACTGTGTGGG CATTACAGCT GACCTCGCAA GACTGCACGC AGAATTACTT ACAGCTTCAG
GACTCACCGC AGGGTCACGG AAATTCAAGA TTTCAGTTCT GTGGCAGAAA TGCTTCGGCT
GTGCCAGTGT TTTATTCTTC TATGAGTACT GCAATGGTCA TTTTCAAATC TGGAGTTGTA
AACAGAAACT CTAGAATGAG TTTCACCTAT CAGATTGCAG ATTGCAACAG AGACTATCAC
AAGGCATTTG GCAACCTGAG AAGCCCTGGA TGGCCAGATA ACTACGACAA TGACAAGGAT
TGCACCGTTA CTCTCACAGC CCCCCAGAAC CACACCATTT CCCTCTTTTT TCATTCACTT
GGCATCGAGA ACTCAGTTGA ATGCAGAAAC GATTTCTTGG AGGTGAGAAA TGGAAGTAAC
AGCAATTCAC CATTACTGGG CAAGTACTGT GGAACTCTGC TGCCAAACCC TGTCTTCTCT
CAAAATAATG AACTATACCT ACGATTTAAG AGTGATAGTG TAACTTCTGA TCGTGGATAT
GAAATCATCT GGACTTCATC ACCCTCTGGA TGTGGTGGAA CTCTTTATGG AGACAGAGGC
TCATTCACCA GCCCCGGCTA TCCAGGCACA TACCCAAACA ACACGTACTG CGAGTGGGTC
CTTGTTGCTC CTGCTGGAAG GCTTGTCACC ATCAACTTCT ACTTCATCAG CATTGACGAT
CCAGGAGACT GTGTCCAGAA CTATCTCACA CTCTATGATG GGCCCAACGC CAGCTCTCCA
TCCTCTGGAC CATACTGCGG AGGCGACACC AGCATAGCTC CCTTCGTGGC TTCCTCAAAT
CAGGTCTTCA TAAAATTTCA TGCTGATTAT GCACGGCGTC CATCCGCATT CCGATTAACT
TGGGACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001072.2
CDS53..10924
Misc Feature(1)155..160(+)
Misc Feature(2)458..553(+)
Misc Feature(3)560..682(+)
Misc Feature(4)560..628(+)
Misc Feature(5)839..946(+)
Misc Feature(6)839..898(+)
Misc Feature(7)965..1081(+)
Misc Feature(8)965..1030(+)
Misc Feature(9)1109..1213(+)
Misc Feature(10)1247..1342(+)
Misc Feature(11)1346..1456(+)
Misc Feature(12)1346..1399(+)
Misc Feature(13)1472..1807(+)
Misc Feature(14)1496..1807(+)
Misc Feature(15)1820..2149(+)
Misc Feature(16)1844..2146(+)
Misc Feature(17)2174..2497(+)
Misc Feature(18)2198..2497(+)
Misc Feature(19)2501..2833(+)
Misc Feature(20)2522..2833(+)
Misc Feature(21)2846..3175(+)
Misc Feature(22)2876..3175(+)
Misc Feature(23)3221..3526(+)
Misc Feature(24)3224..3523(+)
Misc Feature(25)3545..3877(+)
Misc Feature(26)3569..3877(+)
Misc Feature(27)3896..4216(+)
Misc Feature(28)3914..4216(+)
Misc Feature(29)4223..4567(+)
Misc Feature(30)4253..4567(+)
Misc Feature(31)4580..4903(+)
Misc Feature(32)4604..4903(+)
Misc Feature(33)4910..5251(+)
Misc Feature(34)4937..5251(+)
Misc Feature(35)5264..5593(+)
Misc Feature(36)5288..5590(+)
Misc Feature(37)5627..5938(+)
Misc Feature(38)5630..5938(+)
Misc Feature(39)5984..6316(+)
Misc Feature(40)6014..6313(+)
Misc Feature(41)6326..6688(+)
Misc Feature(42)6356..6688(+)
Misc Feature(43)6701..7051(+)
Misc Feature(44)6737..7051(+)
Misc Feature(45)7058..7393(+)
Misc Feature(46)7082..7393(+)
Misc Feature(47)7406..7744(+)
Misc Feature(48)7436..7744(+)
Misc Feature(49)7760..8110(+)
Misc Feature(50)7787..8110(+)
Misc Feature(51)8117..8452(+)
Misc Feature(52)8141..8452(+)
Misc Feature(53)8465..8806(+)
Misc Feature(54)8489..8806(+)
Misc Feature(55)8810..9154(+)
Misc Feature(56)8834..9154(+)
Misc Feature(57)9074..9076(+)
Misc Feature(58)9161..9496(+)
Misc Feature(59)9185..9496(+)
Misc Feature(60)9521..9871(+)
Misc Feature(61)9548..9871(+)
Misc Feature(62)9884..10228(+)
Misc Feature(63)9914..10228(+)
Misc Feature(64)10235..10570(+)
Misc Feature(65)10259..10570(+)
Misc Feature(66)10583..10915(+)
Misc Feature(67)10613..10912(+)
Misc Feature(68)563..691(+)
Misc Feature(69)1103..1247(+)
Misc Feature(70)1248..1348(+)
Misc Feature(71)1349..1462(+)
Misc Feature(72)1472..1819(+)
Misc Feature(73)1820..2173(+)
Misc Feature(74)2174..2500(+)
Misc Feature(75)2501..2845(+)
Misc Feature(76)2846..3193(+)
Misc Feature(77)3194..3544(+)
Misc Feature(78)4223..4579(+)
Misc Feature(79)4910..5263(+)
Misc Feature(80)5984..6325(+)
Misc Feature(81)6701..7057(+)
Misc Feature(82)7058..7405(+)
Misc Feature(83)7406..7759(+)
Misc Feature(84)7760..8116(+)
Misc Feature(85)8465..8809(+)
Misc Feature(86)8810..9160(+)
Misc Feature(87)9161..9520(+)
Misc Feature(88)9521..9883(+)
Misc Feature(89)10235..10582(+)
Misc Feature(90)10583..10921(+)
Exon (1)1..174
Gene:CUBN
Gene Synonym:
Exon (2)175..304
Gene:CUBN
Gene Synonym:
Exon (3)305..400
Gene:CUBN
Gene Synonym:
Exon (4)401..439
Gene:CUBN
Gene Synonym:
Exon (5)440..541
Gene:CUBN
Gene Synonym:
Exon (6)542..645
Gene:CUBN
Gene Synonym:
Exon (7)646..772
Gene:CUBN
Gene Synonym:
Exon (8)773..935
Gene:CUBN
Gene Synonym:
Exon (9)936..1067
Gene:CUBN
Gene Synonym:
Exon (10)1068..1163
Gene:CUBN
Gene Synonym:
Exon (11)1164..1282
Gene:CUBN
Gene Synonym:
Exon (12)1283..1469
Gene:CUBN
Gene Synonym:
Exon (13)1470..1582
Gene:CUBN
Gene Synonym:
Exon (14)1583..1817
Gene:CUBN
Gene Synonym:
Exon (15)1818..1999
Gene:CUBN
Gene Synonym:
Exon (16)2000..2162
Gene:CUBN
Gene Synonym:
Exon (17)2163..2353
Gene:CUBN
Gene Synonym:
Exon (18)2354..2498
Gene:CUBN
Gene Synonym:
Exon (19)2499..2677
Gene:CUBN
Gene Synonym:
Exon (20)2678..2843
Gene:CUBN
Gene Synonym:
Exon (21)2844..3060
Gene:CUBN
Gene Synonym:
Exon (22)3061..3191
Gene:CUBN
Gene Synonym:
Exon (23)3192..3381
Gene:CUBN
Gene Synonym:
Exon (24)3382..3542
Gene:CUBN
Gene Synonym:
Exon (25)3543..3724
Gene:CUBN
Gene Synonym:
Exon (26)3725..3881
Gene:CUBN
Gene Synonym:
Exon (27)3882..4069
Gene:CUBN
Gene Synonym:
Exon (28)4070..4220
Gene:CUBN
Gene Synonym:
Exon (29)4221..4402
Gene:CUBN
Gene Synonym:
Exon (30)4403..4577
Gene:CUBN
Gene Synonym:
Exon (31)4578..4747
Gene:CUBN
Gene Synonym:
Exon (32)4748..4907
Gene:CUBN
Gene Synonym:
Exon (33)4908..5021
Gene:CUBN
Gene Synonym:
Exon (34)5022..5132
Gene:CUBN
Gene Synonym:
Exon (35)5133..5261
Gene:CUBN
Gene Synonym:
Exon (36)5262..5394
Gene:CUBN
Gene Synonym:
Exon (37)5395..5600
Gene:CUBN
Gene Synonym:
Exon (38)5601..5785
Gene:CUBN
Gene Synonym:
Exon (39)5786..5978
Gene:CUBN
Gene Synonym:
Exon (40)5979..6176
Gene:CUBN
Gene Synonym:
Exon (41)6177..6323
Gene:CUBN
Gene Synonym:
Exon (42)6324..6514
Gene:CUBN
Gene Synonym:
Exon (43)6515..6698
Gene:CUBN
Gene Synonym:
Exon (44)6699..6873
Gene:CUBN
Gene Synonym:
Exon (45)6874..7052
Gene:CUBN
Gene Synonym:
Exon (46)7053..7262
Gene:CUBN
Gene Synonym:
Exon (47)7263..7403
Gene:CUBN
Gene Synonym:
Exon (48)7404..7585
Gene:CUBN
Gene Synonym:
Exon (49)7586..7757
Gene:CUBN
Gene Synonym:
Exon (50)7758..7964
Gene:CUBN
Gene Synonym:
Exon (51)7965..8114
Gene:CUBN
Gene Synonym:
Exon (52)8115..8236
Gene:CUBN
Gene Synonym:
Exon (53)8237..8462
Gene:CUBN
Gene Synonym:
Exon (54)8463..8650
Gene:CUBN
Gene Synonym:
Exon (55)8651..8807
Gene:CUBN
Gene Synonym:
Exon (56)8808..8957
Gene:CUBN
Gene Synonym:
Exon (57)8958..9158
Gene:CUBN
Gene Synonym:
Exon (58)9159..9288
Gene:CUBN
Gene Synonym:
Exon (59)9289..9506
Gene:CUBN
Gene Synonym:
Exon (60)9507..9715
Gene:CUBN
Gene Synonym:
Exon (61)9716..9878
Gene:CUBN
Gene Synonym:
Exon (62)9879..10084
Gene:CUBN
Gene Synonym:
Exon (63)10085..10232
Gene:CUBN
Gene Synonym:
Exon (64)10233..10414
Gene:CUBN
Gene Synonym:
Exon (65)10415..10580
Gene:CUBN
Gene Synonym:
Exon (66)10581..10816
Gene:CUBN
Gene Synonym:
Exon (67)10817..11933
Gene:CUBN
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001081
Organism Homo sapiens (human)
Definition Homo sapiens cubilin (intrinsic factor-cobalamin receptor) (CUBN), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001081

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
8581
8641
8701
8761
8821
8881
8941
9001
9061
9121
9181
9241
9301
9361
9421
9481
9541
9601
9661
9721
9781
9841
9901
9961
10021
10081
10141
10201
10261
10321
10381
10441
10501
10561
10621
10681
10741
10801
10861
ATGATGAACA TGTCTTTACC TTTTCTTTGG AGTTTGCTTA CCTTATTAAT ATTTGCTGAA 
GTAAATGGCG AAGCTGGAGA ACTTGAGCTG CAGAGACAAA AAAGAAGCAT CAATCTCCAA
CAGCCTCGAA TGGCTACAGA GAGAGGAAAT TTGGTGTTTC TTACGGGGTC TGCTCAAAAC
ATTGAGTTTA GAACCGGATC CCTGGGAAAA ATTAAATTAA ATGATGAAGA TCTCAGTGAG
TGTTTACATC AGATCCAGAA AAACAAAGAA GATATTATAG AGTTAAAAGG GAGTGCAATT
GGTCTGCCTC AAAATATATC TAGTCAAATC TATCAGCTTA ATTCCAAGCT GGTGGATCTT
GAGAGAAAAT TCCAAGGCTT GCAGCAGACT GTTGACAAAA AGGTTTGCAG CAGCAATCCT
TGCCAGAATG GTGGAACCTG CCTCAATCTG CATGATTCCT TTTTTTGTAT CTGTCCCCCA
CAGTGGAAGG GTCCTCTCTG CTCAGCTGAT GTTAACGAAT GTGAGATTTA CTCAGGAACA
CCCTTGAGCT GCCAGAATGG AGGCACATGT GTTAATACAA TGGGAAGTTA CAGTTGTCAC
TGCCCACCTG AGACGTACGG ACCCCAGTGT GCATCCAAAT ATGACGACTG TGAAGGGGGT
TCTGTGGCAC GCTGTGTCCA TGGCATCTGT GAGGATTTAA TGCGAGAGCA AGCTGGAGAG
CCCAAGTACA GCTGCGTCTG TGATGCTGGG TGGATGTTTT CACCCAACAG CCCTGCCTGC
ACGCTGGACA GAGACGAGTG CAGCTTCCAG CCCGGGCCTT GCTCCACACT TGTGCAGTGT
TTCAACACTC AAGGCTCTTT CTACTGTGGG GCCTGTCCAA CAGGCTGGCA AGGCAATGGA
TATATTTGCG AAGATATCAA TGAATGTGAG ATAAATAACG GCGGCTGTTC TGTGGCTCCA
CCCGTTGAGT GTGTGAATAC ACCTGGGTCT TCCCACTGCC AGGCCTGTCC ACCAGGGTAC
CAGGGTGACG GAAGAGTGTG CACACTCACA GACATCTGCT CAGTCAGTAA TGGAGGCTGC
CACCCAGATG CCTCATGCTC CTCAACTCTA GGTTCCTTAC CTCTCTGCAC GTGTCTCCCG
GGTTATACTG GAAATGGTTA TGGGCCAAAT GGATGTGTGC AGCTCAGTAA TATTTGCCTA
AGTCACCCCT GTCTAAATGG ACAATGCATC GACACTGTCT CTGGTTATTT TTGTAAGTGT
GACTCAGGTT GGACAGGTGT CAACTGTACA GAAAACATCA ATGAGTGTTT GAGCAACCCC
TGTTTGAATG GAGGAACTTG TGTTGATGGC GTTGATTCTT TCAGTTGTGA ATGCACACGT
CTCTGGACTG GAGCTCTCTG TCAGGTTCCT CAGCAAGTTT GTGGAGAGTC CCTCTCAGGA
ATAAATGGAA GCTTCAGCTA CAGGAGCCCG GATGTTGGTT ATGTTCATGA TGTTAACTGC
TTCTGGGTTA TCAAAACTGA AATGGGAAAG GTCCTGCGTA TCACTTTCAC TTTTTTCCGG
TTAGAATCCA TGGACAACTG TCCACACGAG TTTCTTCAGG TTTATGATGG AGATTCCTCT
TCTGCTTTTC AACTTGGAAG ATTTTGTGGC TCCAGCCTCC CTCATGAACT CCTCAGCAGT
GACAATGCTC TCTATTTTCA TCTCTATTCT GAACATTTAA GAAATGGGAG AGGCTTTACA
GTAAGATGGG AAACACAGCA ACCAGAGTGT GGAGGTATCC TGACTGGTCC TTACGGTTCT
ATTAAGTCTC CGGGGTATCC TGGAAACTAT CCCCCAGGAA GAGATTGTGT CTGGATTGTT
GTAACTAGTC CTGACCTCCT GGTAACATTT ACTTTTGGGA CCTTGAGCCT CGAGCACCAT
GATGACTGCA ACAAAGATTA CCTTGAGATT CGAGATGGTC CTTTGTATCA GGACCCCCTT
CTTGGGAAGT TCTGCACCAC TTTCTCTGTC CCACCGCTCC AGACTACTGG CCCCTTTGCC
AGAATTCACT TCCATTCAGA CTCCCAGATT AGTGACCAAG GCTTCCATAT CACCTACTTA
ACATCACCTT CGGATCTGCG TTGTGGTGGG AACTACACGG ACCCAGAGGG TGAACTCTTC
TTGCCTGAGT TGTCTGGGCC TTTCACTCAC ACCAGGCAAT GCGTCTATAT GATGAAGCAG
CCCCAGGGAG AACAAATACA AATCAACTTC ACCCACGTGG AGCTGCAATG CCAGAGTGAC
AGTTCTCAGA ATTACATTGA GGTTCGAGAT GGTGAAACCT TACTTGGAAA AGTCTGTGGC
AACGGAACCA TCTCTCACAT TAAATCCATT ACTAATAGTG TCTGGATCAG GTTTAAAATA
GATGCTTCTG TTGAAAAAGC TAGTTTCAGA GCTGTTTATC AAGTCGCTTG CGGGGATGAA
TTAACTGGAG AAGGGGTCAT TCGCTCGCCT TTTTTTCCTA ACGTGTATCC TGGAGAAAGA
ACCTGTAGGT GGACCATCCA CCAGCCCCAA AGCCAAGTCA TTCTCCTCAA CTTCACTGTC
TTTGAAATTG GAAGTTCTGC CCACTGTGAA ACAGATTATG TTGAGATTGG TAGCAGTTCC
ATTTTGGGTT CTCCTGAAAA TAAAAAGTAT TGCGGTACAG ACATACCTTC ATTTATAACA
TCTGTGTACA ATTTTCTTTA TGTCACATTC GTGAAAAGTT CTTCTACTGA AAACCATGGT
TTCATGGCTA AGTTCAGTGC TGAGGATTTG GCATGTGGAG AAATTCTTAC AGAATCAACA
GGGACCATTC AAAGTCCTGG CCATCCAAAT GTCTACCCCC ACGGTATCAA CTGTACTTGG
CATATATTAG TCCAACCTAA TCACCTGATT CATTTAATGT TCGAAACATT TCATCTGGAG
TTTCATTACA ATTGCACAAA CGACTACTTG GAAGTTTATG ACACCGACTC TGAGACATCC
CTTGGAAGAT ACTGTGGAAA GTCGATCCCG CCATCTCTCA CAAGCAGTGG TAACTCATTG
ATGCTGGTGT TTGTGACTGA CTCCGACCTC GCTTATGAAG GCTTCTTAAT AAACTATGAA
GCAATCAGTG CAGCAACAGC ATGTTTGCAA GACTACACAG ATGATTTGGG GACATTCACT
TCTCCAAACT TCCCCAATAA TTATCCCAAC AACTGGGAAT GCATTTATCG GATCACAGTG
AGAACTGGCC AACTGATTGC AGTGCACTTC ACAAACTTCT CCTTGGAGGA AGCCATTGGA
AACTATTATA CAGATTTTCT GGAAATCAGA GATGGAGGCT ATGAAAAATC ACCATTGCTG
GGAATATTCT ATGGCTCAAA TCTACCCCCA ACAATCATCT CTCATAGTAA CAAACTATGG
TTAAAATTTA AGAGTGACCA AATAGACACA AGGTCTGGAT TCTCAGCTTA CTGGGATGGG
TCATCAACAG GTTGCGGGGG TAATCTCACC ACTTCAAGCG GCACGTTCAT ATCTCCCAAC
TACCCGATGC CCTATTACCA CAGCTCTGAA TGCTACTGGT GGTTGAAATC TAGCCACGGC
AGCGCATTTG AACTGGAATT CAAAGACTTT CACTTGGAGC ATCATCCAAA CTGCACTTTA
GATTACCTGG CTGTATATGA TGGCCCAAGT AGCAACTCTC ATCTGCTAAC TCAGCTTTGT
GGGGATGAGA AACCCCCTCT TATTCGTTCT AGTGGAGACA GCATGTTTAT AAAACTGAGG
ACAGATGAAG GTCAGCAAGG ACGTGGCTTC AAGGCTGAAT ACCGGCAGAC ATGTGAGAAT
GTGGTAATAG TCAATCAAAC CTATGGCATC TTAGAGAGTA TAGGGTATCC GAATCCTTAT
TCTGAAAATC AGCATTGCAA CTGGACCATC CGGGCAACAA CAGGCAACAC TGTGAACTAC
ACATTTTTAG CATTTGACTT GGAACATCAC ATAAACTGCT CCACAGATTA TTTAGAGCTC
TATGATGGAC CACGGCAGAT GGGACGCTAC TGTGGAGTAG ACCTGCCCCC TCCAGGGAGT
ACTACAAGCT CCAAGCTTCA AGTGCTGCTC CTTACAGATG GGGTTGGCCG CCGTGAGAAA
GGATTTCAGA TGCAGTGGTT TGTTTACGGT TGTGGTGGAG AGCTGTCTGG GGCCACAGGC
TCCTTCAGCA GCCCCGGGTT CCCCAACAGG TATCCACCAA ACAAGGAGTG TATCTGGTAC
ATTAGGACGG ACCCCGGGAG TAGCATTCAG CTCACCATCC ATGACTTCGA TGTGGAGTAT
CATTCAAGGT GCAACTTTGA TGTCTTGGAG ATCTATGGAG GCCCCGATTT CCACTCTCCC
AGAATAGCCC AACTGTGTAC CCAGAGATCA CCTGAGAACC CCATGCAGGT CTCCAGCACT
GGAAATGAGC TAGCAATTCG ATTCAAGACC GACTTGTCCA TAAATGGGAG AGGCTTCAAT
GCGTCATGGC AAGCAGTCAC TGGAGGTTGT GGTGGGATTT TCCAGGCTCC CAGTGGAGAG
ATTCATTCTC CAAATTACCC CAGTCCTTAT AGGAGCAACA CAGACTGTTC TTGGGTCATT
CGGGTTGACA GAAATCATCG TGTTCTCTTG AACTTCACTG ACTTTGATCT TGAACCACAA
GACTCTTGTA TTATGGCATA CGATGGCTTA AGCTCCACAA TGTCCCGCCT TGCCAGGACG
TGTGGAAGGG AGCAGCTGGC TAACCCCATC GTCTCCTCAG GAAACAGCCT CTTCTTGAGA
TTTCAGTCTG GCCCTTCCAG ACAGAACAGA GGCTTCCGAG CTCAATTCAG GCAAGCCTGC
GGAGGCCACA TCCTCACCAG CTCATTTGAT ACTGTTTCCT CTCCACGGTT CCCTGCCAAT
TATCCAAACA ATCAGAACTG CAGCTGGATC ATTCAAGCGC AACCTCCATT AAATCATATC
ACCCTCTCTT TTACCCACTT TGAACTTGAA AGAAGCACAA CGTGTGCACG TGACTTTGTA
GAAATTTTGG ATGGCGGCCA CGAAGACGCG CCCCTCCGAG GCCGTTACTG TGGCACCGAC
ATGCCCCATC CTATCACATC CTTCAGCAGC GCCCTGACGC TGAGATTCGT CTCTGATTCT
AGCATCAGTG CTGGGGGTTT CCACACCACG GTCACCGCAT CAGTGTCGGC TTGTGGTGGA
ACGTTCTACA TGGCTGAAGG CATCTTCAAC AGCCCTGGCT ACCCAGACAT TTATCCCCCT
AATGTGGAAT GTGTCTGGAA CATCGTCAGT TCCCCTGGCA ACCGGCTCCA GCTGTCTTTT
ATATCTTTCC AGTTGGAAGA CTCTCAGGAC TGCAGCAGAG ATTTTGTGGA GATCCGTGAA
GGAAATGCCA CGGGTCACTT GGTGGGACGA TACTGTGGAA ACTCCTTCCC TCTCAATTAT
TCTTCCATCG TTGGACATAC CCTGTGGGTC AGATTTATCT CAGATGGTTC TGGCAGCGGC
ACGGGCTTCC AGGCCACATT TATGAAGATA TTTGGCAATG ATAATATTGT GGGAACTCAT
GGGAAAGTCG CCTCTCCTTT CTGGCCTGAA AACTACCCAC ATAACTCCAA TTACCAATGG
ACAGTAAATG TGAATGCATC TCACGTTGTC CATGGTAGAA TCTTGGAGAT GGACATAGAA
GAAATACAAA ACTGCTATTA TGACAAATTA AGGATCTATG ATGGGCCTAG CATTCACGCC
CGCCTAATTG GAGCTTACTG TGGTACCCAG ACTGAATCTT TCAGCTCCAC TGGAAATTCT
TTGACATTTC ATTTTTACTC CGACTCTTCA ATCTCAGGGA AGGGATTCCT TCTGGAGTGG
TTTGCAGTGG ATGCACCTGA TGGTGTTTTA CCTACCATTG CTCCAGGTGC TTGTGGTGGC
TTCCTGAGGA CGGGAGATGC ACCCGTGTTT CTCTTCTCCC CGGGCTGGCC TGACAGTTAC
AGTAATAGAG TGGACTGTAC GTGGCTCATC CAGGCTCCCG ACTCTACCGT GGAACTCAAC
ATTCTTTCCC TGGACATTGA ATCTCACCGA ACGTGTGCCT ATGATAGCCT TGTGATACGA
GATGGAGATA ATAACTTGGC CCAGCAGCTA GCAGTTCTCT GTGGCAGAGA GATCCCTGGG
CCCATCCGGT CTACTGGAGA GTACATGTTC ATCCGCTTCA CCTCGGACTC CAGTGTAACC
AGGGCAGGCT TCAATGCATC CTTTCACAAG AGCTGCGGTG GATATTTGCA TGCAGACAGA
GGGATCATCA CGTCCCCCAA GTATCCAGAG ACTTACCCAT CCAACCTCAA CTGTTCTTGG
CACGTCCTGG TCCAAAGTGG CCTGACCATT GCTGTCCATT TTGAACAGCC TTTCCAGATT
CCAAATGGAG ATTCTTCTTG CAACCAGGGG GATTACTTGG TGCTAAGAAA TGGTCCTGAT
ATCTGTTCTC CACCCTTGGG ACCCCCTGGA GGAAATGGTC ATTTTTGTGG CAGTCATGCT
TCATCAACTC TGTTCACCTC GGATAATCAA ATGTTTGTTC AGTTTATTTC TGATCACAGT
AATGAAGGGC AAGGATTTAA AATCAAATAT GAGGCAAAGA GTTTAGCCTG TGGGGGCAAC
GTCTACATCC ATGATGCTGA TTCTGCTGGG TATGTGACCT CCCCCAACCA CCCTCATAAT
TATCCCCCGC ACGCTGATTG CATTTGGATC TTAGCGGCTC CACCGGAAAC ACGCATACAG
CTGCAATTTG AAGATCGATT CGATATTGAA GTAACACCCA ACTGTACTTC CAACTACCTT
GAGTTGCGGG ATGGAGTGGA TTCGGATGCA CCAATACTTT CCAAATTTTG TGGGACATCT
TTGCCCAGCA GTCAGTGGTC CTCAGGAGAG GTTATGTATT TGAGATTTCG ATCTGACAAC
AGCCCCACAC ATGTGGGATT CAAGGCCAAG TATTCTATAG CTCAGTGTGG GGGAAGAGTA
CCAGGGCAAA GTGGTGTTGT TGAAAGCATT GGACATCCAA CACTTCCATA CAGAGACAAC
TTATTCTGTG AGTGGCATCT CCAGGGGCTC TCTGGACACT ATCTCACCAT CTCTTTTGAA
GACTTTAACC TTCAGAATTC TTCTGGCTGT GAAAAAGACT TCGTGGAGAT CTGGGACAAT
CATACCTCTG GAAACATCTT GGGCAGATAC TGTGGAAACA CCATTCCTGA CAGCATAGAC
ACTTCTAGCA ATACTGCTGT GGTCAGGTTT GTCACAGACG GCTCTGTGAC TGCCTCAGGA
TTCAGACTGC GATTTGAATC CAGTATGGAA GAGTGTGGTG GGGATCTTCA GGGCTCTATT
GGAACATTTA CTTCTCCCAA CTACCCGAAC CCAAATCCTC ATGGCCGGAT CTGCGAGTGG
AGAATCACTG CCCCGGAGGG AAGGCGGATC ACCCTAATGT TTAACAACCT GAGGCTGGCC
ACGCATCCGT CCTGCAACAA TGAGCATGTG ATAGTATTCA ATGGCATTAG AAGTAACTCA
CCCCAGCTAG AGAAACTGTG TAGTAGTGTG AATGTAAGCA ATGAGATTAA ATCTTCAGGA
AACACAATGA AAGTCATTTT TTTCACGGAT GGATCCAGGC CATATGGCGG CTTCACTGCT
TCCTATACCT CCAGTGAAGA TGCAGTGTGT GGTGGGTCTC TTCCAAATAC TCCTGAAGGA
AACTTTACTT CTCCTGGCTA TGACGGAGTC AGGAATTACT CAAGAAACCT GAACTGCGAA
TGGACTCTCA GCAATCCAAA TCAGGGAAAT TCATCCATTT CCATTCACTT TGAAGATTTT
TACCTAGAAA GTCACCAAGA CTGTCAATTT GATGTCCTCG AGTTTCGAGT GGGTGATGCT
GATGGGCCCC TGATGTGGAG ACTTTGTGGT CCTTCAAAGC CTACATTGCC ATTGGTTATA
CCTTATTCTC AGGTATGGAT TCACTTTGTC ACCAACGAAC GTGTAGAACA CATTGGATTC
CATGCAAAGT ATTCCTTTAC AGATTGTGGC GGAATACAGA TAGGTGACAG TGGAGTGATC
ACAAGCCCCA ACTATCCAAA TGCTTATGAC AGCCTGACCC ACTGCTCTTC GCTGTTGGAG
GCCCCACAAG GGCACACCAT CACTCTCACA TTTAGTGACT TTGATATTGA ACCCCATACA
ACTTGTGCTT GGGACTCTGT CACTGTCAGG AATGGTGGGT CCCCTGAATC ACCCATCATA
GGACAATACT GTGGAAATTC AAACCCCAGG ACAATACAGT CAGGTTCCAA TCAGCTGGTC
GTGACTTTTA ACTCAGACCA TTCATTGCAA GGTGGTGGAT TTTATGCTAC GTGGAACACA
CAAACTTTAG GTTGTGGTGG AATATTTCAT TCTGATAATG GTACAATCAG ATCCCCTCAC
TGGCCTCAGA ATTTTCCCGA AAACAGCAGA TGTTCCTGGA CGGCCATTAC TCACAAAAGT
AAACACTTGG AGATCAGCTT TGACAACAAC TTCCTAATCC CCAGCGGTGA TGGACAATGT
CAGAATAGCT TCGTGAAGGT GTGGGCAGGA ACTGAGGAGG TGGACAAAGC CCTGCTAGCC
ACTGGCTGTG GGAACGTGGC TCCGGGTCCC GTTATCACAC CAAGTAACAC ATTCACTGCC
GTCTTCCAGT CTCAGGAGGC ACCAGCTCAG GGCTTCTCCG CGTCCTTTGT TAGCCGATGT
GGAAGTAATT TCACTGGCCC TTCAGGTTAC ATCATTTCTC CAAATTACCC AAAACAATAT
GACAACAACA TGAATTGCAC CTATGTCATA GAGGCTAATC CTCTGTCAGT GGTCCTCTTG
ACTTTTGTGT CCTTCCACTT AGAAGCTCGT TCCGCTGTGA CGGGAAGCTG TGTCAACGAT
GGCGTGCACA TTATCAGAGG TTACAGCGTC ATGTCCACCC CATTTGCTAC TGTGTGTGGG
GATGAGATGC CAGCTCCCCT CACCATCGCT GGGCCGGTTC TGCTTAACTT CTACTCCAAC
GAGCAAATCA CAGACTTCGG ATTCAAGTTT TCCTATAGGA TAATCTCCTG TGGTGGTGTG
TTCAATTTCT CTTCTGGAAT CATCACAAGT CCTGCCTATT CATACGCAGA CTACCCAAAT
GATATGCACT GTCTGTATAC CATCACCGTT AGTGACGACA AGGTGATCGA GCTCAAGTTC
AGTGATTTTG ATGTGGTTCC CTCCACCTCC TGCTCCCATG ACTACCTGGC AATTTACGAT
GGTGCCAATA CCAGCGATCC CCTTCTTGGC AAATTCTGCG GTTCCAAGCG CCCACCAAAT
GTGAAGAGCA GCAATAATAG TATGCTCCTG GTGTTCAAGA CAGATTCATT TCAGACAGCA
AAAGGCTGGA AGATGTCTTT CCGGCAGACA TTGGGGCCTC AGCAAGGATG TGGTGGTTAT
CTGACAGGCT CGAATAATAC CTTTGCCTCT CCTGATTCTG ATTCGAATGG AATGTATGAC
AAGAATTTAA ACTGTGTATG GATCATAATT GCACCTGTAA ACAAAGTAAT TCACCTCACC
TTCAATACAT TTGCTCTGGA GGCAGCAAGT ACTAGGCAAA GATGCCTTTA TGATTATGTA
AAGTTATATG ATGGGGATAG TGAAAATGCG AACTTGGCTG GAACGTTTTG TGGTTCCACA
GTACCTGCTC CTTTTATCTC TTCTGGTAAC TTCCTTACGG TTCAATTCAT CAGTGACTTA
ACATTAGAGA GGGAAGGATT TAATGCTACA TACACCATCA TGGACATGCC TTGTGGTGGA
ACATACAATG CAACTTGGAC CCCACAAAAT ATTTCATCAC CCAATTCATC AGACCCAGAT
GTCCCATTTT CCATCTGTAC TTGGGTCATT GATTCCCCTC CGCATCAGCA GGTCAAGATA
ACTGTGTGGG CATTACAGCT GACCTCGCAA GACTGCACGC AGAATTACTT ACAGCTTCAG
GACTCACCGC AGGGTCACGG AAATTCAAGA TTTCAGTTCT GTGGCAGAAA TGCTTCGGCT
GTGCCAGTGT TTTATTCTTC TATGAGTACT GCAATGGTCA TTTTCAAATC TGGAGTTGTA
AACAGAAACT CTAGAATGAG TTTCACCTAT CAGATTGCAG ATTGCAACAG AGACTATCAC
AAGGCATTTG GCAACCTGAG AAGCCCTGGA TGGCCAGATA ACTACGACAA TGACAAGGAT
TGCACCGTTA CTCTCACAGC CCCCCAGAAC CACACCATTT CCCTCTTTTT TCATTCACTT
GGCATCGAGA ACTCAGTTGA ATGCAGAAAC GATTTCTTGG AGGTGAGAAA TGGAAGTAAC
AGCAATTCAC CATTACTGGG CAAGTACTGT GGAACTCTGC TGCCAAACCC TGTCTTCTCT
CAAAATAATG AACTATACCT ACGATTTAAG AGTGATAGTG TAACTTCTGA TCGTGGATAT
GAAATCATCT GGACTTCATC ACCCTCTGGA TGTGGTGGAA CTCTTTATGG AGACAGAGGC
TCATTCACCA GCCCCGGCTA TCCAGGCACA TACCCAAACA ACACGTACTG CGAGTGGGTC
CTTGTTGCTC CTGCTGGAAG GCTTGTCACC ATCAACTTCT ACTTCATCAG CATTGACGAT
CCAGGAGACT GTGTCCAGAA CTATCTCACA CTCTATGATG GGCCCAACGC CAGCTCTCCA
TCCTCTGGAC CATACTGCGG AGGCGACACC AGCATAGCTC CCTTCGTGGC TTCCTCAAAT
CAGGTCTTCA TAAAATTTCA TGCTGATTAT GCACGGCGTC CATCCGCATT CCGATTAACT
TGGGACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61599
Clone ID Related Accession (Same CDS sequence) XM_011519708
Accession Version XM_011519708.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 8604bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cubilin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008705.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
8581
ATGATGAACA TGTCTTTACC TTTTCTTTGG AGTTTGCTTA CCTTATTAAT ATTTGCTGAA 
GTAAATGGCG AAGCTGGAGA ACTTGAGCTG CAGAGACAAA AAAGAAGCAT CAATCTCCAA
CAGCCTCGAA TGGCTACAGA GAGAGGAAAT TTGGTGTTTC TTACGGGGTC TGCTCAAAAC
ATTGAGTTTA GAACCGGATC CCTGGGAAAA ATTAAATTAA ATGATGAAGA TCTCAGTGAG
TGTTTACATC AGATCCAGAA AAACAAAGAA GATATTATAG AGTTAAAAGG GAGTGCAATT
GGTCTGCCTC AAAATATATC TAGTCAAATC TATCAGCTTA ATTCCAAGCT GGTGGATCTT
GAGAGAAAAT TCCAAGGCTT GCAGCAGACT GTTGACAAAA AGGTTTGCAG CAGCAATCCT
TGCCAGAATG GTGGAACCTG CCTCAATCTG CATGATTCCT TTTTTTGTAT CTGTCCCCCA
CAGTGGAAGG GTCCTCTCTG CTCAGCTGAT GTTAACGAAT GTGAGATTTA CTCAGGAACA
CCCTTGAGCT GCCAGAATGG AGGCACATGT GTTAATACAA TGGGAAGTTA CAGTTGTCAC
TGCCCACCTG AGACGTACGG ACCCCAGTGT GCATCCAAAT ATGACGACTG TGAAGGGGGT
TCTGTGGCAC GCTGTGTCCA TGGCATCTGT GAGGATTTAA TGCGAGAGCA AGCTGGAGAG
CCCAAGTACA GCTGCGTCTG TGATGCTGGG TGGATGTTTT CACCCAACAG CCCTGCCTGC
ACGCTGGACA GAGACGAGTG CAGCTTCCAG CCCGGGCCTT GCTCCACACT TGTGCAGTGT
TTCAACACTC AAGGCTCTTT CTACTGTGGG GCCTGTCCAA CAGGCTGGCA AGGCAATGGA
TATATTTGCG AAGATATCAA TGAATGTGAG ATAAATAACG GCGGCTGTTC TGTGGCTCCA
CCCGTTGAGT GTGTGAATAC ACCTGGGTCT TCCCACTGCC AGGCCTGTCC ACCAGGGTAC
CAGGGTGACG GAAGAGTGTG CACACTCACA GACATCTGCT CAGTCAGTAA TGGAGGCTGC
CACCCAGATG CCTCATGCTC CTCAACTCTA GGTTCCTTAC CTCTCTGCAC GTGTCTCCCG
GGTTATACTG GAAATGGTTA TGGGCCAAAT GGATGTGTGC AGCTCAGTAA TATTTGCCTA
AGTCACCCCT GTCTAAATGG ACAATGCATC GACACTGTCT CTGGTTATTT TTGTAAGTGT
GACTCAGGTT GGACAGGTGT CAACTGTACA GAAAACATCA ATGAGTGTTT GAGCAACCCC
TGTTTGAATG GAGGAACTTG TGTTGATGGC GTTGATTCTT TCAGTTGTGA ATGCACACGT
CTCTGGACTG GAGCTCTCTG TCAGGTTCCT CAGCAAGTTT GTGGAGAGTC CCTCTCAGGA
ATAAATGGAA GCTTCAGCTA CAGGAGCCCG GATGTTGGTT ATGTTCATGA TGTTAACTGC
TTCTGGGTTA TCAAAACTGA AATGGGAAAG GTCCTGCGTA TCACTTTCAC TTTTTTCCGG
TTAGAATCCA TGGACAACTG TCCACACGAG TTTCTTCAGG TTTATGATGG AGATTCCTCT
TCTGCTTTTC AACTTGGAAG ATTTTGTGGC TCCAGCCTCC CTCATGAACT CCTCAGCAGT
GACAATGCTC TCTATTTTCA TCTCTATTCT GAACATTTAA GAAATGGGAG AGGCTTTACA
GTAAGATGGG AAACACAGCA ACCAGAGTGT GGAGGTATCC TGACTGGTCC TTACGGTTCT
ATTAAGTCTC CGGGGTATCC TGGAAACTAT CCCCCAGGAA GAGATTGTGT CTGGATTGTT
GTAACTAGTC CTGACCTCCT GGTAACATTT ACTTTTGGGA CCTTGAGCCT CGAGCACCAT
GATGACTGCA ACAAAGATTA CCTTGAGATT CGAGATGGTC CTTTGTATCA GGACCCCCTT
CTTGGGAAGT TCTGCACCAC TTTCTCTGTC CCACCGCTCC AGACTACTGG CCCCTTTGCC
AGAATTCACT TCCATTCAGA CTCCCAGATT AGTGACCAAG GCTTCCATAT CACCTACTTA
ACATCACCTT CGGATCTGCG TTGTGGTGGG AACTACACGG ACCCAGAGGG TGAACTCTTC
TTGCCTGAGT TGTCTGGGCC TTTCACTCAC ACCAGGCAAT GCGTCTATAT GATGAAGCAG
CCCCAGGGAG AACAAATACA AATCAACTTC ACCCACGTGG AGCTGCAATG CCAGAGTGAC
AGTTCTCAGA ATTACATTGA GGTTCGAGAT GGTGAAACCT TACTTGGAAA AGTCTGTGGC
AACGGAACCA TCTCTCACAT TAAATCCATT ACTAATAGTG TCTGGATCAG GTTTAAAATA
GATGCTTCTG TTGAAAAAGC TAGTTTCAGA GCTGTTTATC AAGTCGCTTG CGGGGATGAA
TTAACTGGAG AAGGGGTCAT TCGCTCGCCT TTTTTTCCTA ACGTGTATCC TGGAGAAAGA
ACCTGTAGGT GGACCATCCA CCAGCCCCAA AGCCAAGTCA TTCTCCTCAA CTTCACTGTC
TTTGAAATTG GAAGTTCTGC CCACTGTGAA ACAGATTATG TTGAGATTGG TAGCAGTTCC
ATTTTGGGTT CTCCTGAAAA TAAAAAGTAT TGCGGTACAG ACATACCTTC ATTTATAACA
TCTGTGTACA ATTTTCTTTA TGTCACATTC GTGAAAAGTT CTTCTACTGA AAACCATGGT
TTCATGGCTA AGTTCAGTGC TGAGGATTTG GCATGTGGAG AAATTCTTAC AGAATCAACA
GGGACCATTC AAAGTCCTGG CCATCCAAAT GTCTACCCCC ACGGTATCAA CTGTACTTGG
CATATATTAG TCCAACCTAA TCACCTGATT CATTTAATGT TCGAAACATT TCATCTGGAG
TTTCATTACA ATTGCACAAA CGACTACTTG GAAGTTTATG ACACCGACTC TGAGACATCC
CTTGGAAGAT ACTGTGGAAA GTCGATCCCG CCATCTCTCA CAAGCAGTGG TAACTCATTG
ATGCTGGTGT TTGTGACTGA CTCCGACCTC GCTTATGAAG GCTTCTTAAT AAACTATGAA
GCAATCAGTG CAGCAACAGC ATGTTTGCAA GACTACACAG ATGATTTGGG GACATTCACT
TCTCCAAACT TCCCCAATAA TTATCCCAAC AACTGGGAAT GCATTTATCG GATCACAGTG
AGAACTGGCC AACTGATTGC AGTGCACTTC ACAAACTTCT CCTTGGAGGA AGCCATTGGA
AACTATTATA CAGATTTTCT GGAAATCAGA GATGGAGGCT ATGAAAAATC ACCATTGCTG
GGAATATTCT ATGGCTCAAA TCTACCCCCA ACAATCATCT CTCATAGTAA CAAACTATGG
TTAAAATTTA AGAGTGACCA AATAGACACA AGGTCTGGAT TCTCAGCTTA CTGGGATGGG
TCATCAACAG GTTGCGGGGG TAATCTCACC ACTTCAAGCG GCACGTTCAT ATCTCCCAAC
TACCCGATGC CCTATTACCA CAGCTCTGAA TGCTACTGGT GGTTGAAATC TAGCCACGGC
AGCGCATTTG AACTGGAATT CAAAGACTTT CACTTGGAGC ATCATCCAAA CTGCACTTTA
GATTACCTGG CTGTATATGA TGGCCCAAGT AGCAACTCTC ATCTGCTAAC TCAGCTTTGT
GGGGATGAGA AACCCCCTCT TATTCGTTCT AGTGGAGACA GCATGTTTAT AAAACTGAGG
ACAGATGAAG GTCAGCAAGG ACGTGGCTTC AAGGCTGAAT ACCGGCAGAC ATGTGAGAAT
GTGGTAATAG TCAATCAAAC CTATGGCATC TTAGAGAGTA TAGGGTATCC GAATCCTTAT
TCTGAAAATC AGCATTGCAA CTGGACCATC CGGGCAACAA CAGGCAACAC TGTGAACTAC
ACATTTTTAG CATTTGACTT GGAACATCAC ATAAACTGCT CCACAGATTA TTTAGAGCTC
TATGATGGAC CACGGCAGAT GGGACGCTAC TGTGGAGTAG ACCTGCCCCC TCCAGGGAGT
ACTACAAGCT CCAAGCTTCA AGTGCTGCTC CTTACAGATG GGGTTGGCCG CCGTGAGAAA
GGATTTCAGA TGCAGTGGTT TGTTTACGGT TGTGGTGGAG AGCTGTCTGG GGCCACAGGC
TCCTTCAGCA GCCCCGGGTT CCCCAACAGG TATCCACCAA ACAAGGAGTG TATCTGGTAC
ATTAGGACGG ACCCCGGGAG TAGCATTCAG CTCACCATCC ATGACTTCGA TGTGGAGTAT
CATTCAAGGT GCAACTTTGA TGTCTTGGAG ATCTATGGAG GCCCCGATTT CCACTCTCCC
AGAATAGCCC AACTGTGTAC CCAGAGATCA CCTGAGAACC CCATGCAGGT CTCCAGCACT
GGAAATGAGC TAGCAATTCG ATTCAAGACC GACTTGTCCA TAAATGGGAG AGGCTTCAAT
GCGTCATGGC AAGCAGTCAC TGGAGGTTGT GGTGGGATTT TCCAGGCTCC CAGTGGAGAG
ATTCATTCTC CAAATTACCC CAGTCCTTAT AGGAGCAACA CAGACTGTTC TTGGGTCATT
CGGGTTGACA GAAATCATCG TGTTCTCTTG AACTTCACTG ACTTTGATCT TGAACCACAA
GACTCTTGTA TTATGGCATA CGATGGCTTA AGCTCCACAA TGTCCCGCCT TGCCAGGACG
TGTGGAAGGG AGCAGCTGGC TAACCCCATC GTCTCCTCAG GAAACAGCCT CTTCTTGAGA
TTTCAGTCTG GCCCTTCCAG ACAGAACAGA GGCTTCCGAG CTCAATTCAG GCAAGCCTGC
GGAGGCCACA TCCTCACCAG CTCATTTGAT ACTGTTTCCT CTCCACGGTT CCCTGCCAAT
TATCCAAACA ATCAGAACTG CAGCTGGATC ATTCAAGCGC AACCTCCATT AAATCATATC
ACCCTCTCTT TTACCCACTT TGAACTTGAA AGAAGCACAA CGTGTGCACG TGACTTTGTA
GAAATTTTGG ATGGCGGCCA CGAAGACGCG CCCCTCCGAG GCCGTTACTG TGGCACCGAC
ATGCCCCATC CTATCACATC CTTCAGCAGC GCCCTGACGC TGAGATTCGT CTCTGATTCT
AGCATCAGTG CTGGGGGTTT CCACACCACG GTCACCGCAT CAGTGTCGGC TTGTGGTGGA
ACGTTCTACA TGGCTGAAGG CATCTTCAAC AGCCCTGGCT ACCCAGACAT TTATCCCCCT
AATGTGGAAT GTGTCTGGAA CATCGTCAGT TCCCCTGGCA ACCGGCTCCA GCTGTCTTTT
ATATCTTTCC AGTTGGAAGA CTCTCAGGAC TGCAGCAGAG ATTTTGTGGA GATCCGTGAA
GGAAATGCCA CGGGTCACTT GGTGGGACGA TACTGTGGAA ACTCCTTCCC TCTCAATTAT
TCTTCCATCG TTGGACATAC CCTGTGGGTC AGATTTATCT CAGATGGTTC TGGCAGCGGC
ACGGGCTTCC AGGCCACATT TATGAAGATA TTTGGCAATG ATAATATTGT GGGAACTCAT
GGGAAAGTCG CCTCTCCTTT CTGGCCTGAA AACTACCCAC ATAACTCCAA TTACCAATGG
ACAGTAAATG TGAATGCATC TCACGTTGTC CATGGTAGAA TCTTGGAGAT GGACATAGAA
GAAATACAAA ACTGCTATTA TGACAAATTA AGGATCTATG ATGGGCCTAG CATTCACGCC
CGCCTAATTG GAGCTTACTG TGGTACCCAG ACTGAATCTT TCAGCTCCAC TGGAAATTCT
TTGACATTTC ATTTTTACTC CGACTCTTCA ATCTCAGGGA AGGGATTCCT TCTGGAGTGG
TTTGCAGTGG ATGCACCTGA TGGTGTTTTA CCTACCATTG CTCCAGGTGC TTGTGGTGGC
TTCCTGAGGA CGGGAGATGC ACCCGTGTTT CTCTTCTCCC CGGGCTGGCC TGACAGTTAC
AGTAATAGAG TGGACTGTAC GTGGCTCATC CAGGCTCCCG ACTCTACCGT GGAACTCAAC
ATTCTTTCCC TGGACATTGA ATCTCACCGA ACGTGTGCCT ATGATAGCCT TGTGATACGA
GATGGAGATA ATAACTTGGC CCAGCAGCTA GCAGTTCTCT GTGGCAGAGA GATCCCTGGG
CCCATCCGGT CTACTGGAGA GTACATGTTC ATCCGCTTCA CCTCGGACTC CAGTGTAACC
AGGGCAGGCT TCAATGCATC CTTTCACAAG AGCTGCGGTG GATATTTGCA TGCAGACAGA
GGGATCATCA CGTCCCCCAA GTATCCAGAG ACTTACCCAT CCAACCTCAA CTGTTCTTGG
CACGTCCTGG TCCAAAGTGG CCTGACCATT GCTGTCCATT TTGAACAGCC TTTCCAGATT
CCAAATGGAG ATTCTTCTTG CAACCAGGGG GATTACTTGG TGCTAAGAAA TGGTCCTGAT
ATCTGTTCTC CACCCTTGGG ACCCCCTGGA GGAAATGGTC ATTTTTGTGG CAGTCATGCT
TCATCAACTC TGTTCACCTC GGATAATCAA ATGTTTGTTC AGTTTATTTC TGATCACAGT
AATGAAGGGC AAGGATTTAA AATCAAATAT GAGGCAAAGA GTTTAGCCTG TGGGGGCAAC
GTCTACATCC ATGATGCTGA TTCTGCTGGG TATGTGACCT CCCCCAACCA CCCTCATAAT
TATCCCCCGC ACGCTGATTG CATTTGGATC TTAGCGGCTC CACCGGAAAC ACGCATACAG
CTGCAATTTG AAGATCGATT CGATATTGAA GTAACACCCA ACTGTACTTC CAACTACCTT
GAGTTGCGGG ATGGAGTGGA TTCGGATGCA CCAATACTTT CCAAATTTTG TGGGACATCT
TTGCCCAGCA GTCAGTGGTC CTCAGGAGAG GTTATGTATT TGAGATTTCG ATCTGACAAC
AGCCCCACAC ATGTGGGATT CAAGGCCAAG TATTCTATAG CTCAGTGTGG GGGAAGAGTA
CCAGGGCAAA GTGGTGTTGT TGAAAGCATT GGACATCCAA CACTTCCATA CAGAGACAAC
TTATTCTGTG AGTGGCATCT CCAGGGGCTC TCTGGACACT ATCTCACCAT CTCTTTTGAA
GACTTTAACC TTCAGAATTC TTCTGGCTGT GAAAAAGACT TCGTGGAGAT CTGGGACAAT
CATACCTCTG GAAACATCTT GGGCAGATAC TGTGGAAACA CCATTCCTGA CAGCATAGAC
ACTTCTAGCA ATACTGCTGT GGTCAGGTTT GTCACAGACG GCTCTGTGAC TGCCTCAGGA
TTCAGACTGC GATTTGAATC CAGTATGGAA GAGTGTGGTG GGGATCTTCA GGGCTCTATT
GGAACATTTA CTTCTCCCAA CTACCCGAAC CCAAATCCTC ATGGCCGGAT CTGCGAGTGG
AGAATCACTG CCCCGGAGGG AAGGCGGATC ACCCTAATGT TTAACAACCT GAGGCTGGCC
ACGCATCCGT CCTGCAACAA TGAGCATGTG ATAGTATTCA ATGGCATTAG AAGTAACTCA
CCCCAGCTAG AGAAACTGTG TAGTAGTGTG AATGTAAGCA ATGAGATTAA ATCTTCAGGA
AACACAATGA AAGTCATTTT TTTCACGGAT GGATCCAGGC CATATGGCGG CTTCACTGCT
TCCTATACCT CCAGTGAAGA TGCAGTGTGT GGTGGGTCTC TTCCAAATAC TCCTGAAGGA
AACTTTACTT CTCCTGGCTA TGACGGAGTC AGGAATTACT CAAGAAACCT GAACTGCGAA
TGGACTCTCA GCAATCCAAA TCAGGGAAAT TCATCCATTT CCATTCACTT TGAAGATTTT
TACCTAGAAA GTCACCAAGA CTGTCAATTT GATGTCCTCG AGTTTCGAGT GGGTGATGCT
GATGGGCCCC TGATGTGGAG ACTTTGTGGT CCTTCAAAGC CTACATTGCC ATTGGTTATA
CCTTATTCTC AGGTATGGAT TCACTTTGTC ACCAACGAAC GTGTAGAACA CATTGGATTC
CATGCAAAGT ATTCCTTTAC AGATTGTGGC GGAATACAGA TAGGTGACAG TGGAGTGATC
ACAAGCCCCA ACTATCCAAA TGCTTATGAC AGCCTGACCC ACTGCTCTTC GCTGTTGGAG
GCCCCACAAG GGCACACCAT CACTCTCACA TTTAGTGACT TTGATATTGA ACCCCATACA
ACTTGTGCTT GGGACTCTGT CACTGTCAGG AATGGTGGGT CCCCTGAATC ACCCATCATA
GGACAATACT GTGGAAATTC AAACCCCAGG ACAATACAGT CAGGTTCCAA TCAGCTGGTC
GTGACTTTTA ACTCAGACCA TTCATTGCAA GGTGGTGGAT TTTATGCTAC GTGGAACACA
CAAACTTTAG GTTGTGGTGG AATATTTCAT TCTGATAATG GTACAATCAG ATCCCCTCAC
TGGCCTCAGA ATTTTCCCGA AAACAGCAGA TGTTCCTGGA CGGCCATTAC TCACAAAAGT
AAACACTTGG AGATCAGCTT TGACAACAAC TTCCTAATCC CCAGCGGTGA TGGACAATGT
CAGAATAGCT TCGTGAAGGA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518010.1
CDS485..9088
Misc Feature(1)893..985(+)
Misc Feature(2)992..1114(+)
Misc Feature(3)992..1060(+)
Misc Feature(4)1271..1378(+)
Misc Feature(5)1271..1330(+)
Misc Feature(6)1397..1513(+)
Misc Feature(7)1397..1462(+)
Misc Feature(8)1541..1645(+)
Misc Feature(9)1679..1774(+)
Misc Feature(10)1778..1888(+)
Misc Feature(11)1778..1831(+)
Misc Feature(12)1904..2239(+)
Misc Feature(13)1928..2239(+)
Misc Feature(14)2252..2581(+)
Misc Feature(15)2276..2578(+)
Misc Feature(16)2606..2929(+)
Misc Feature(17)2630..2929(+)
Misc Feature(18)2933..3265(+)
Misc Feature(19)2954..3265(+)
Misc Feature(20)3278..3607(+)
Misc Feature(21)3308..3607(+)
Misc Feature(22)3653..3958(+)
Misc Feature(23)3656..3955(+)
Misc Feature(24)3977..4309(+)
Misc Feature(25)4001..4309(+)
Misc Feature(26)4328..4648(+)
Misc Feature(27)4346..4648(+)
Misc Feature(28)4655..4999(+)
Misc Feature(29)4685..4999(+)
Misc Feature(30)5012..5335(+)
Misc Feature(31)5036..5335(+)
Misc Feature(32)5342..5683(+)
Misc Feature(33)5369..5683(+)
Misc Feature(34)5696..6025(+)
Misc Feature(35)5720..6022(+)
Misc Feature(36)6059..6370(+)
Misc Feature(37)6062..6370(+)
Misc Feature(38)6416..6748(+)
Misc Feature(39)6446..6745(+)
Misc Feature(40)6758..7120(+)
Misc Feature(41)6788..7120(+)
Misc Feature(42)7133..7483(+)
Misc Feature(43)7169..7483(+)
Misc Feature(44)7490..7825(+)
Misc Feature(45)7514..7825(+)
Misc Feature(46)7838..8176(+)
Misc Feature(47)7868..8176(+)
Misc Feature(48)8192..8542(+)
Misc Feature(49)8219..8542(+)
Misc Feature(50)8549..8884(+)
Misc Feature(51)8573..8884(+)
Misc Feature(52)8897..>9082(+)
Misc Feature(53)8921..9031(+)
Translation

Target ORF information:

RefSeq Version XM_011519708
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cubilin (intrinsic factor-cobalamin receptor) (CUBN), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011519708

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
8581
ATGATGAACA TGTCTTTACC TTTTCTTTGG AGTTTGCTTA CCTTATTAAT ATTTGCTGAA 
GTAAATGGCG AAGCTGGAGA ACTTGAGCTG CAGAGACAAA AAAGAAGCAT CAATCTCCAA
CAGCCTCGAA TGGCTACAGA GAGAGGAAAT TTGGTGTTTC TTACGGGGTC TGCTCAAAAC
ATTGAGTTTA GAACCGGATC CCTGGGAAAA ATTAAATTAA ATGATGAAGA TCTCAGTGAG
TGTTTACATC AGATCCAGAA AAACAAAGAA GATATTATAG AGTTAAAAGG GAGTGCAATT
GGTCTGCCTC AAAATATATC TAGTCAAATC TATCAGCTTA ATTCCAAGCT GGTGGATCTT
GAGAGAAAAT TCCAAGGCTT GCAGCAGACT GTTGACAAAA AGGTTTGCAG CAGCAATCCT
TGCCAGAATG GTGGAACCTG CCTCAATCTG CATGATTCCT TTTTTTGTAT CTGTCCCCCA
CAGTGGAAGG GTCCTCTCTG CTCAGCTGAT GTTAACGAAT GTGAGATTTA CTCAGGAACA
CCCTTGAGCT GCCAGAATGG AGGCACATGT GTTAATACAA TGGGAAGTTA CAGTTGTCAC
TGCCCACCTG AGACGTACGG ACCCCAGTGT GCATCCAAAT ATGACGACTG TGAAGGGGGT
TCTGTGGCAC GCTGTGTCCA TGGCATCTGT GAGGATTTAA TGCGAGAGCA AGCTGGAGAG
CCCAAGTACA GCTGCGTCTG TGATGCTGGG TGGATGTTTT CACCCAACAG CCCTGCCTGC
ACGCTGGACA GAGACGAGTG CAGCTTCCAG CCCGGGCCTT GCTCCACACT TGTGCAGTGT
TTCAACACTC AAGGCTCTTT CTACTGTGGG GCCTGTCCAA CAGGCTGGCA AGGCAATGGA
TATATTTGCG AAGATATCAA TGAATGTGAG ATAAATAACG GCGGCTGTTC TGTGGCTCCA
CCCGTTGAGT GTGTGAATAC ACCTGGGTCT TCCCACTGCC AGGCCTGTCC ACCAGGGTAC
CAGGGTGACG GAAGAGTGTG CACACTCACA GACATCTGCT CAGTCAGTAA TGGAGGCTGC
CACCCAGATG CCTCATGCTC CTCAACTCTA GGTTCCTTAC CTCTCTGCAC GTGTCTCCCG
GGTTATACTG GAAATGGTTA TGGGCCAAAT GGATGTGTGC AGCTCAGTAA TATTTGCCTA
AGTCACCCCT GTCTAAATGG ACAATGCATC GACACTGTCT CTGGTTATTT TTGTAAGTGT
GACTCAGGTT GGACAGGTGT CAACTGTACA GAAAACATCA ATGAGTGTTT GAGCAACCCC
TGTTTGAATG GAGGAACTTG TGTTGATGGC GTTGATTCTT TCAGTTGTGA ATGCACACGT
CTCTGGACTG GAGCTCTCTG TCAGGTTCCT CAGCAAGTTT GTGGAGAGTC CCTCTCAGGA
ATAAATGGAA GCTTCAGCTA CAGGAGCCCG GATGTTGGTT ATGTTCATGA TGTTAACTGC
TTCTGGGTTA TCAAAACTGA AATGGGAAAG GTCCTGCGTA TCACTTTCAC TTTTTTCCGG
TTAGAATCCA TGGACAACTG TCCACACGAG TTTCTTCAGG TTTATGATGG AGATTCCTCT
TCTGCTTTTC AACTTGGAAG ATTTTGTGGC TCCAGCCTCC CTCATGAACT CCTCAGCAGT
GACAATGCTC TCTATTTTCA TCTCTATTCT GAACATTTAA GAAATGGGAG AGGCTTTACA
GTAAGATGGG AAACACAGCA ACCAGAGTGT GGAGGTATCC TGACTGGTCC TTACGGTTCT
ATTAAGTCTC CGGGGTATCC TGGAAACTAT CCCCCAGGAA GAGATTGTGT CTGGATTGTT
GTAACTAGTC CTGACCTCCT GGTAACATTT ACTTTTGGGA CCTTGAGCCT CGAGCACCAT
GATGACTGCA ACAAAGATTA CCTTGAGATT CGAGATGGTC CTTTGTATCA GGACCCCCTT
CTTGGGAAGT TCTGCACCAC TTTCTCTGTC CCACCGCTCC AGACTACTGG CCCCTTTGCC
AGAATTCACT TCCATTCAGA CTCCCAGATT AGTGACCAAG GCTTCCATAT CACCTACTTA
ACATCACCTT CGGATCTGCG TTGTGGTGGG AACTACACGG ACCCAGAGGG TGAACTCTTC
TTGCCTGAGT TGTCTGGGCC TTTCACTCAC ACCAGGCAAT GCGTCTATAT GATGAAGCAG
CCCCAGGGAG AACAAATACA AATCAACTTC ACCCACGTGG AGCTGCAATG CCAGAGTGAC
AGTTCTCAGA ATTACATTGA GGTTCGAGAT GGTGAAACCT TACTTGGAAA AGTCTGTGGC
AACGGAACCA TCTCTCACAT TAAATCCATT ACTAATAGTG TCTGGATCAG GTTTAAAATA
GATGCTTCTG TTGAAAAAGC TAGTTTCAGA GCTGTTTATC AAGTCGCTTG CGGGGATGAA
TTAACTGGAG AAGGGGTCAT TCGCTCGCCT TTTTTTCCTA ACGTGTATCC TGGAGAAAGA
ACCTGTAGGT GGACCATCCA CCAGCCCCAA AGCCAAGTCA TTCTCCTCAA CTTCACTGTC
TTTGAAATTG GAAGTTCTGC CCACTGTGAA ACAGATTATG TTGAGATTGG TAGCAGTTCC
ATTTTGGGTT CTCCTGAAAA TAAAAAGTAT TGCGGTACAG ACATACCTTC ATTTATAACA
TCTGTGTACA ATTTTCTTTA TGTCACATTC GTGAAAAGTT CTTCTACTGA AAACCATGGT
TTCATGGCTA AGTTCAGTGC TGAGGATTTG GCATGTGGAG AAATTCTTAC AGAATCAACA
GGGACCATTC AAAGTCCTGG CCATCCAAAT GTCTACCCCC ACGGTATCAA CTGTACTTGG
CATATATTAG TCCAACCTAA TCACCTGATT CATTTAATGT TCGAAACATT TCATCTGGAG
TTTCATTACA ATTGCACAAA CGACTACTTG GAAGTTTATG ACACCGACTC TGAGACATCC
CTTGGAAGAT ACTGTGGAAA GTCGATCCCG CCATCTCTCA CAAGCAGTGG TAACTCATTG
ATGCTGGTGT TTGTGACTGA CTCCGACCTC GCTTATGAAG GCTTCTTAAT AAACTATGAA
GCAATCAGTG CAGCAACAGC ATGTTTGCAA GACTACACAG ATGATTTGGG GACATTCACT
TCTCCAAACT TCCCCAATAA TTATCCCAAC AACTGGGAAT GCATTTATCG GATCACAGTG
AGAACTGGCC AACTGATTGC AGTGCACTTC ACAAACTTCT CCTTGGAGGA AGCCATTGGA
AACTATTATA CAGATTTTCT GGAAATCAGA GATGGAGGCT ATGAAAAATC ACCATTGCTG
GGAATATTCT ATGGCTCAAA TCTACCCCCA ACAATCATCT CTCATAGTAA CAAACTATGG
TTAAAATTTA AGAGTGACCA AATAGACACA AGGTCTGGAT TCTCAGCTTA CTGGGATGGG
TCATCAACAG GTTGCGGGGG TAATCTCACC ACTTCAAGCG GCACGTTCAT ATCTCCCAAC
TACCCGATGC CCTATTACCA CAGCTCTGAA TGCTACTGGT GGTTGAAATC TAGCCACGGC
AGCGCATTTG AACTGGAATT CAAAGACTTT CACTTGGAGC ATCATCCAAA CTGCACTTTA
GATTACCTGG CTGTATATGA TGGCCCAAGT AGCAACTCTC ATCTGCTAAC TCAGCTTTGT
GGGGATGAGA AACCCCCTCT TATTCGTTCT AGTGGAGACA GCATGTTTAT AAAACTGAGG
ACAGATGAAG GTCAGCAAGG ACGTGGCTTC AAGGCTGAAT ACCGGCAGAC ATGTGAGAAT
GTGGTAATAG TCAATCAAAC CTATGGCATC TTAGAGAGTA TAGGGTATCC GAATCCTTAT
TCTGAAAATC AGCATTGCAA CTGGACCATC CGGGCAACAA CAGGCAACAC TGTGAACTAC
ACATTTTTAG CATTTGACTT GGAACATCAC ATAAACTGCT CCACAGATTA TTTAGAGCTC
TATGATGGAC CACGGCAGAT GGGACGCTAC TGTGGAGTAG ACCTGCCCCC TCCAGGGAGT
ACTACAAGCT CCAAGCTTCA AGTGCTGCTC CTTACAGATG GGGTTGGCCG CCGTGAGAAA
GGATTTCAGA TGCAGTGGTT TGTTTACGGT TGTGGTGGAG AGCTGTCTGG GGCCACAGGC
TCCTTCAGCA GCCCCGGGTT CCCCAACAGG TATCCACCAA ACAAGGAGTG TATCTGGTAC
ATTAGGACGG ACCCCGGGAG TAGCATTCAG CTCACCATCC ATGACTTCGA TGTGGAGTAT
CATTCAAGGT GCAACTTTGA TGTCTTGGAG ATCTATGGAG GCCCCGATTT CCACTCTCCC
AGAATAGCCC AACTGTGTAC CCAGAGATCA CCTGAGAACC CCATGCAGGT CTCCAGCACT
GGAAATGAGC TAGCAATTCG ATTCAAGACC GACTTGTCCA TAAATGGGAG AGGCTTCAAT
GCGTCATGGC AAGCAGTCAC TGGAGGTTGT GGTGGGATTT TCCAGGCTCC CAGTGGAGAG
ATTCATTCTC CAAATTACCC CAGTCCTTAT AGGAGCAACA CAGACTGTTC TTGGGTCATT
CGGGTTGACA GAAATCATCG TGTTCTCTTG AACTTCACTG ACTTTGATCT TGAACCACAA
GACTCTTGTA TTATGGCATA CGATGGCTTA AGCTCCACAA TGTCCCGCCT TGCCAGGACG
TGTGGAAGGG AGCAGCTGGC TAACCCCATC GTCTCCTCAG GAAACAGCCT CTTCTTGAGA
TTTCAGTCTG GCCCTTCCAG ACAGAACAGA GGCTTCCGAG CTCAATTCAG GCAAGCCTGC
GGAGGCCACA TCCTCACCAG CTCATTTGAT ACTGTTTCCT CTCCACGGTT CCCTGCCAAT
TATCCAAACA ATCAGAACTG CAGCTGGATC ATTCAAGCGC AACCTCCATT AAATCATATC
ACCCTCTCTT TTACCCACTT TGAACTTGAA AGAAGCACAA CGTGTGCACG TGACTTTGTA
GAAATTTTGG ATGGCGGCCA CGAAGACGCG CCCCTCCGAG GCCGTTACTG TGGCACCGAC
ATGCCCCATC CTATCACATC CTTCAGCAGC GCCCTGACGC TGAGATTCGT CTCTGATTCT
AGCATCAGTG CTGGGGGTTT CCACACCACG GTCACCGCAT CAGTGTCGGC TTGTGGTGGA
ACGTTCTACA TGGCTGAAGG CATCTTCAAC AGCCCTGGCT ACCCAGACAT TTATCCCCCT
AATGTGGAAT GTGTCTGGAA CATCGTCAGT TCCCCTGGCA ACCGGCTCCA GCTGTCTTTT
ATATCTTTCC AGTTGGAAGA CTCTCAGGAC TGCAGCAGAG ATTTTGTGGA GATCCGTGAA
GGAAATGCCA CGGGTCACTT GGTGGGACGA TACTGTGGAA ACTCCTTCCC TCTCAATTAT
TCTTCCATCG TTGGACATAC CCTGTGGGTC AGATTTATCT CAGATGGTTC TGGCAGCGGC
ACGGGCTTCC AGGCCACATT TATGAAGATA TTTGGCAATG ATAATATTGT GGGAACTCAT
GGGAAAGTCG CCTCTCCTTT CTGGCCTGAA AACTACCCAC ATAACTCCAA TTACCAATGG
ACAGTAAATG TGAATGCATC TCACGTTGTC CATGGTAGAA TCTTGGAGAT GGACATAGAA
GAAATACAAA ACTGCTATTA TGACAAATTA AGGATCTATG ATGGGCCTAG CATTCACGCC
CGCCTAATTG GAGCTTACTG TGGTACCCAG ACTGAATCTT TCAGCTCCAC TGGAAATTCT
TTGACATTTC ATTTTTACTC CGACTCTTCA ATCTCAGGGA AGGGATTCCT TCTGGAGTGG
TTTGCAGTGG ATGCACCTGA TGGTGTTTTA CCTACCATTG CTCCAGGTGC TTGTGGTGGC
TTCCTGAGGA CGGGAGATGC ACCCGTGTTT CTCTTCTCCC CGGGCTGGCC TGACAGTTAC
AGTAATAGAG TGGACTGTAC GTGGCTCATC CAGGCTCCCG ACTCTACCGT GGAACTCAAC
ATTCTTTCCC TGGACATTGA ATCTCACCGA ACGTGTGCCT ATGATAGCCT TGTGATACGA
GATGGAGATA ATAACTTGGC CCAGCAGCTA GCAGTTCTCT GTGGCAGAGA GATCCCTGGG
CCCATCCGGT CTACTGGAGA GTACATGTTC ATCCGCTTCA CCTCGGACTC CAGTGTAACC
AGGGCAGGCT TCAATGCATC CTTTCACAAG AGCTGCGGTG GATATTTGCA TGCAGACAGA
GGGATCATCA CGTCCCCCAA GTATCCAGAG ACTTACCCAT CCAACCTCAA CTGTTCTTGG
CACGTCCTGG TCCAAAGTGG CCTGACCATT GCTGTCCATT TTGAACAGCC TTTCCAGATT
CCAAATGGAG ATTCTTCTTG CAACCAGGGG GATTACTTGG TGCTAAGAAA TGGTCCTGAT
ATCTGTTCTC CACCCTTGGG ACCCCCTGGA GGAAATGGTC ATTTTTGTGG CAGTCATGCT
TCATCAACTC TGTTCACCTC GGATAATCAA ATGTTTGTTC AGTTTATTTC TGATCACAGT
AATGAAGGGC AAGGATTTAA AATCAAATAT GAGGCAAAGA GTTTAGCCTG TGGGGGCAAC
GTCTACATCC ATGATGCTGA TTCTGCTGGG TATGTGACCT CCCCCAACCA CCCTCATAAT
TATCCCCCGC ACGCTGATTG CATTTGGATC TTAGCGGCTC CACCGGAAAC ACGCATACAG
CTGCAATTTG AAGATCGATT CGATATTGAA GTAACACCCA ACTGTACTTC CAACTACCTT
GAGTTGCGGG ATGGAGTGGA TTCGGATGCA CCAATACTTT CCAAATTTTG TGGGACATCT
TTGCCCAGCA GTCAGTGGTC CTCAGGAGAG GTTATGTATT TGAGATTTCG ATCTGACAAC
AGCCCCACAC ATGTGGGATT CAAGGCCAAG TATTCTATAG CTCAGTGTGG GGGAAGAGTA
CCAGGGCAAA GTGGTGTTGT TGAAAGCATT GGACATCCAA CACTTCCATA CAGAGACAAC
TTATTCTGTG AGTGGCATCT CCAGGGGCTC TCTGGACACT ATCTCACCAT CTCTTTTGAA
GACTTTAACC TTCAGAATTC TTCTGGCTGT GAAAAAGACT TCGTGGAGAT CTGGGACAAT
CATACCTCTG GAAACATCTT GGGCAGATAC TGTGGAAACA CCATTCCTGA CAGCATAGAC
ACTTCTAGCA ATACTGCTGT GGTCAGGTTT GTCACAGACG GCTCTGTGAC TGCCTCAGGA
TTCAGACTGC GATTTGAATC CAGTATGGAA GAGTGTGGTG GGGATCTTCA GGGCTCTATT
GGAACATTTA CTTCTCCCAA CTACCCGAAC CCAAATCCTC ATGGCCGGAT CTGCGAGTGG
AGAATCACTG CCCCGGAGGG AAGGCGGATC ACCCTAATGT TTAACAACCT GAGGCTGGCC
ACGCATCCGT CCTGCAACAA TGAGCATGTG ATAGTATTCA ATGGCATTAG AAGTAACTCA
CCCCAGCTAG AGAAACTGTG TAGTAGTGTG AATGTAAGCA ATGAGATTAA ATCTTCAGGA
AACACAATGA AAGTCATTTT TTTCACGGAT GGATCCAGGC CATATGGCGG CTTCACTGCT
TCCTATACCT CCAGTGAAGA TGCAGTGTGT GGTGGGTCTC TTCCAAATAC TCCTGAAGGA
AACTTTACTT CTCCTGGCTA TGACGGAGTC AGGAATTACT CAAGAAACCT GAACTGCGAA
TGGACTCTCA GCAATCCAAA TCAGGGAAAT TCATCCATTT CCATTCACTT TGAAGATTTT
TACCTAGAAA GTCACCAAGA CTGTCAATTT GATGTCCTCG AGTTTCGAGT GGGTGATGCT
GATGGGCCCC TGATGTGGAG ACTTTGTGGT CCTTCAAAGC CTACATTGCC ATTGGTTATA
CCTTATTCTC AGGTATGGAT TCACTTTGTC ACCAACGAAC GTGTAGAACA CATTGGATTC
CATGCAAAGT ATTCCTTTAC AGATTGTGGC GGAATACAGA TAGGTGACAG TGGAGTGATC
ACAAGCCCCA ACTATCCAAA TGCTTATGAC AGCCTGACCC ACTGCTCTTC GCTGTTGGAG
GCCCCACAAG GGCACACCAT CACTCTCACA TTTAGTGACT TTGATATTGA ACCCCATACA
ACTTGTGCTT GGGACTCTGT CACTGTCAGG AATGGTGGGT CCCCTGAATC ACCCATCATA
GGACAATACT GTGGAAATTC AAACCCCAGG ACAATACAGT CAGGTTCCAA TCAGCTGGTC
GTGACTTTTA ACTCAGACCA TTCATTGCAA GGTGGTGGAT TTTATGCTAC GTGGAACACA
CAAACTTTAG GTTGTGGTGG AATATTTCAT TCTGATAATG GTACAATCAG ATCCCCTCAC
TGGCCTCAGA ATTTTCCCGA AAACAGCAGA TGTTCCTGGA CGGCCATTAC TCACAAAAGT
AAACACTTGG AGATCAGCTT TGACAACAAC TTCCTAATCC CCAGCGGTGA TGGACAATGT
CAGAATAGCT TCGTGAAGGA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61600
Clone ID Related Accession (Same CDS sequence) XM_011519709
Accession Version XM_011519709.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6858bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cubilin isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008705.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
ATGCTCTATG ATGGACCACG GCAGATGGGA CGCTACTGTG GAGTAGACCT GCCCCCTCCA 
GGGAGTACTA CAAGCTCCAA GCTTCAAGTG CTGCTCCTTA CAGATGGGGT TGGCCGCCGT
GAGAAAGGAT TTCAGATGCA GTGGTTTGTT TACGGTTGTG GTGGAGAGCT GTCTGGGGCC
ACAGGCTCCT TCAGCAGCCC CGGGTTCCCC AACAGGTATC CACCAAACAA GGAGTGTATC
TGGTACATTA GGACGGACCC CGGGAGTAGC ATTCAGCTCA CCATCCATGA CTTCGATGTG
GAGTATCATT CAAGGTGCAA CTTTGATGTC TTGGAGATCT ATGGAGGCCC CGATTTCCAC
TCTCCCAGAA TAGCCCAACT GTGTACCCAG AGATCACCTG AGAACCCCAT GCAGGTCTCC
AGCACTGGAA ATGAGCTAGC AATTCGATTC AAGACCGACT TGTCCATAAA TGGGAGAGGC
TTCAATGCGT CATGGCAAGC AGTCACTGGA GGTTGTGGTG GGATTTTCCA GGCTCCCAGT
GGAGAGATTC ATTCTCCAAA TTACCCCAGT CCTTATAGGA GCAACACAGA CTGTTCTTGG
GTCATTCGGG TTGACAGAAA TCATCGTGTT CTCTTGAACT TCACTGACTT TGATCTTGAA
CCACAAGACT CTTGTATTAT GGCATACGAT GGCTTAAGCT CCACAATGTC CCGCCTTGCC
AGGACGTGTG GAAGGGAGCA GCTGGCTAAC CCCATCGTCT CCTCAGGAAA CAGCCTCTTC
TTGAGATTTC AGTCTGGCCC TTCCAGACAG AACAGAGGCT TCCGAGCTCA ATTCAGGCAA
GCCTGCGGAG GCCACATCCT CACCAGCTCA TTTGATACTG TTTCCTCTCC ACGGTTCCCT
GCCAATTATC CAAACAATCA GAACTGCAGC TGGATCATTC AAGCGCAACC TCCATTAAAT
CATATCACCC TCTCTTTTAC CCACTTTGAA CTTGAAAGAA GCACAACGTG TGCACGTGAC
TTTGTAGAAA TTTTGGATGG CGGCCACGAA GACGCGCCCC TCCGAGGCCG TTACTGTGGC
ACCGACATGC CCCATCCTAT CACATCCTTC AGCAGCGCCC TGACGCTGAG ATTCGTCTCT
GATTCTAGCA TCAGTGCTGG GGGTTTCCAC ACCACGGTCA CCGCATCAGT GTCGGCTTGT
GGTGGAACGT TCTACATGGC TGAAGGCATC TTCAACAGCC CTGGCTACCC AGACATTTAT
CCCCCTAATG TGGAATGTGT CTGGAACATC GTCAGTTCCC CTGGCAACCG GCTCCAGCTG
TCTTTTATAT CTTTCCAGTT GGAAGACTCT CAGGACTGCA GCAGAGATTT TGTGGAGATC
CGTGAAGGAA ATGCCACGGG TCACTTGGTG GGACGATACT GTGGAAACTC CTTCCCTCTC
AATTATTCTT CCATCGTTGG ACATACCCTG TGGGTCAGAT TTATCTCAGA TGGTTCTGGC
AGCGGCACGG GCTTCCAGGC CACATTTATG AAGATATTTG GCAATGATAA TATTGTGGGA
ACTCATGGGA AAGTCGCCTC TCCTTTCTGG CCTGAAAACT ACCCACATAA CTCCAATTAC
CAATGGACAG TAAATGTGAA TGCATCTCAC GTTGTCCATG GTAGAATCTT GGAGATGGAC
ATAGAAGAAA TACAAAACTG CTATTATGAC AAATTAAGGA TCTATGATGG GCCTAGCATT
CACGCCCGCC TAATTGGAGC TTACTGTGGT ACCCAGACTG AATCTTTCAG CTCCACTGGA
AATTCTTTGA CATTTCATTT TTACTCCGAC TCTTCAATCT CAGGGAAGGG ATTCCTTCTG
GAGTGGTTTG CAGTGGATGC ACCTGATGGT GTTTTACCTA CCATTGCTCC AGGTGCTTGT
GGTGGCTTCC TGAGGACGGG AGATGCACCC GTGTTTCTCT TCTCCCCGGG CTGGCCTGAC
AGTTACAGTA ATAGAGTGGA CTGTACGTGG CTCATCCAGG CTCCCGACTC TACCGTGGAA
CTCAACATTC TTTCCCTGGA CATTGAATCT CACCGAACGT GTGCCTATGA TAGCCTTGTG
ATACGAGATG GAGATAATAA CTTGGCCCAG CAGCTAGCAG TTCTCTGTGG CAGAGAGATC
CCTGGGCCCA TCCGGTCTAC TGGAGAGTAC ATGTTCATCC GCTTCACCTC GGACTCCAGT
GTAACCAGGG CAGGCTTCAA TGCATCCTTT CACAAGAGCT GCGGTGGATA TTTGCATGCA
GACAGAGGGA TCATCACGTC CCCCAAGTAT CCAGAGACTT ACCCATCCAA CCTCAACTGT
TCTTGGCACG TCCTGGTCCA AAGTGGCCTG ACCATTGCTG TCCATTTTGA ACAGCCTTTC
CAGATTCCAA ATGGAGATTC TTCTTGCAAC CAGGGGGATT ACTTGGTGCT AAGAAATGGT
CCTGATATCT GTTCTCCACC CTTGGGACCC CCTGGAGGAA ATGGTCATTT TTGTGGCAGT
CATGCTTCAT CAACTCTGTT CACCTCGGAT AATCAAATGT TTGTTCAGTT TATTTCTGAT
CACAGTAATG AAGGGCAAGG ATTTAAAATC AAATATGAGG CAAAGAGTTT AGCCTGTGGG
GGCAACGTCT ACATCCATGA TGCTGATTCT GCTGGGTATG TGACCTCCCC CAACCACCCT
CATAATTATC CCCCGCACGC TGATTGCATT TGGATCTTAG CGGCTCCACC GGAAACACGC
ATACAGCTGC AATTTGAAGA TCGATTCGAT ATTGAAGTAA CACCCAACTG TACTTCCAAC
TACCTTGAGT TGCGGGATGG AGTGGATTCG GATGCACCAA TACTTTCCAA ATTTTGTGGG
ACATCTTTGC CCAGCAGTCA GTGGTCCTCA GGAGAGGTTA TGTATTTGAG ATTTCGATCT
GACAACAGCC CCACACATGT GGGATTCAAG GCCAAGTATT CTATAGCTCA GTGTGGGGGA
AGAGTACCAG GGCAAAGTGG TGTTGTTGAA AGCATTGGAC ATCCAACACT TCCATACAGA
GACAACTTAT TCTGTGAGTG GCATCTCCAG GGGCTCTCTG GACACTATCT CACCATCTCT
TTTGAAGACT TTAACCTTCA GAATTCTTCT GGCTGTGAAA AAGACTTCGT GGAGATCTGG
GACAATCATA CCTCTGGAAA CATCTTGGGC AGATACTGTG GAAACACCAT TCCTGACAGC
ATAGACACTT CTAGCAATAC TGCTGTGGTC AGGTTTGTCA CAGACGGCTC TGTGACTGCC
TCAGGATTCA GACTGCGATT TGAATCCAGT ATGGAAGAGT GTGGTGGGGA TCTTCAGGGC
TCTATTGGAA CATTTACTTC TCCCAACTAC CCGAACCCAA ATCCTCATGG CCGGATCTGC
GAGTGGAGAA TCACTGCCCC GGAGGGAAGG CGGATCACCC TAATGTTTAA CAACCTGAGG
CTGGCCACGC ATCCGTCCTG CAACAATGAG CATGTGATAG TATTCAATGG CATTAGAAGT
AACTCACCCC AGCTAGAGAA ACTGTGTAGT AGTGTGAATG TAAGCAATGA GATTAAATCT
TCAGGAAACA CAATGAAAGT CATTTTTTTC ACGGATGGAT CCAGGCCATA TGGCGGCTTC
ACTGCTTCCT ATACCTCCAG TGAAGATGCA GTGTGTGGTG GGTCTCTTCC AAATACTCCT
GAAGGAAACT TTACTTCTCC TGGCTATGAC GGAGTCAGGA ATTACTCAAG AAACCTGAAC
TGCGAATGGA CTCTCAGCAA TCCAAATCAG GGAAATTCAT CCATTTCCAT TCACTTTGAA
GATTTTTACC TAGAAAGTCA CCAAGACTGT CAATTTGATG TCCTCGAGTT TCGAGTGGGT
GATGCTGATG GGCCCCTGAT GTGGAGACTT TGTGGTCCTT CAAAGCCTAC ATTGCCATTG
GTTATACCTT ATTCTCAGGT ATGGATTCAC TTTGTCACCA ACGAACGTGT AGAACACATT
GGATTCCATG CAAAGTATTC CTTTACAGAT TGTGGCGGAA TACAGATAGG TGACAGTGGA
GTGATCACAA GCCCCAACTA TCCAAATGCT TATGACAGCC TGACCCACTG CTCTTCGCTG
TTGGAGGCCC CACAAGGGCA CACCATCACT CTCACATTTA GTGACTTTGA TATTGAACCC
CATACAACTT GTGCTTGGGA CTCTGTCACT GTCAGGAATG GTGGGTCCCC TGAATCACCC
ATCATAGGAC AATACTGTGG AAATTCAAAC CCCAGGACAA TACAGTCAGG TTCCAATCAG
CTGGTCGTGA CTTTTAACTC AGACCATTCA TTGCAAGGTG GTGGATTTTA TGCTACGTGG
AACACACAAA CTTTAGGTTG TGGTGGAATA TTTCATTCTG ATAATGGTAC AATCAGATCC
CCTCACTGGC CTCAGAATTT TCCCGAAAAC AGCAGATGTT CCTGGACGGC CATTACTCAC
AAAAGTAAAC ACTTGGAGAT CAGCTTTGAC AACAACTTCC TAATCCCCAG CGGTGATGGA
CAATGTCAGA ATAGCTTCGT GAAGGTGTGG GCAGGAACTG AGGAGGTGGA CAAAGCCCTG
CTAGCCACTG GCTGTGGGAA CGTGGCTCCG GGTCCCGTTA TCACACCAAG TAACACATTC
ACTGCCGTCT TCCAGTCTCA GGAGGCACCA GCTCAGGGCT TCTCCGCGTC CTTTGTTAGC
CGATGTGGAA GTAATTTCAC TGGCCCTTCA GGTTACATCA TTTCTCCAAA TTACCCAAAA
CAATATGACA ACAACATGAA TTGCACCTAT GTCATAGAGG CTAATCCTCT GTCAGTGGTC
CTCTTGACTT TTGTGTCCTT CCACTTAGAA GCTCGTTCCG CTGTGACGGG AAGCTGTGTC
AACGATGGCG TGCACATTAT CAGAGGTTAC AGCGTCATGT CCACCCCATT TGCTACTGTG
TGTGGGGATG AGATGCCAGC TCCCCTCACC ATCGCTGGGC CGGTTCTGCT TAACTTCTAC
TCCAACGAGC AAATCACAGA CTTCGGATTC AAGTTTTCCT ATAGGATAAT CTCCTGTGGT
GGTGTGTTCA ATTTCTCTTC TGGAATCATC ACAAGTCCTG CCTATTCATA CGCAGACTAC
CCAAATGATA TGCACTGTCT GTATACCATC ACCGTTAGTG ACGACAAGGT GATCGAGCTC
AAGTTCAGTG ATTTTGATGT GGTTCCCTCC ACCTCCTGCT CCCATGACTA CCTGGCAATT
TACGATGGTG CCAATACCAG CGATCCCCTT CTTGGCAAAT TCTGCGGTTC CAAGCGCCCA
CCAAATGTGA AGAGCAGCAA TAATAGTATG CTCCTGGTGT TCAAGACAGA TTCATTTCAG
ACAGCAAAAG GCTGGAAGAT GTCTTTCCGG CAGACATTGG GGCCTCAGCA AGGATGTGGT
GGTTATCTGA CAGGCTCGAA TAATACCTTT GCCTCTCCTG ATTCTGATTC GAATGGAATG
TATGACAAGA ATTTAAACTG TGTATGGATC ATAATTGCAC CTGTAAACAA AGTAATTCAC
CTCACCTTCA ATACATTTGC TCTGGAGGCA GCAAGTACTA GGCAAAGATG CCTTTATGAT
TATGTAAAGT TATATGATGG GGATAGTGAA AATGCGAACT TGGCTGGAAC GTTTTGTGGT
TCCACAGTAC CTGCTCCTTT TATCTCTTCT GGTAACTTCC TTACGGTTCA ATTCATCAGT
GACTTAACAT TAGAGAGGGA AGGATTTAAT GCTACATACA CCATCATGGA CATGCCTTGT
GGTGGAACAT ACAATGCAAC TTGGACCCCA CAAAATATTT CATCACCCAA TTCATCAGAC
CCAGATGTCC CATTTTCCAT CTGTACTTGG GTCATTGATT CCCCTCCGCA TCAGCAGGTC
AAGATAACTG TGTGGGCATT ACAGCTGACC TCGCAAGACT GCACGCAGAA TTACTTACAG
CTTCAGGACT CACCGCAGGG TCACGGAAAT TCAAGATTTC AGTTCTGTGG CAGAAATGCT
TCGGCTGTGC CAGTGTTTTA TTCTTCTATG AGTACTGCAA TGGTCATTTT CAAATCTGGA
GTTGTAAACA GAAACTCTAG AATGAGTTTC ACCTATCAGA TTGCAGATTG CAACAGAGAC
TATCACAAGG CATTTGGCAA CCTGAGAAGC CCTGGATGGC CAGATAACTA CGACAATGAC
AAGGATTGCA CCGTTACTCT CACAGCCCCC CAGAACCACA CCATTTCCCT CTTTTTTCAT
TCACTTGGCA TCGAGAACTC AGTTGAATGC AGAAACGATT TCTTGGAGGT GAGAAATGGA
AGTAACAGCA ATTCACCATT ACTGGGCAAG TACTGTGGAA CTCTGCTGCC AAACCCTGTC
TTCTCTCAAA ATAATGAACT ATACCTACGA TTTAAGAGTG ATAGTGTAAC TTCTGATCGT
GGATATGAAA TCATCTGGAC TTCATCACCC TCTGGATGTG GTGGAACTCT TTATGGAGAC
AGAGGCTCAT TCACCAGCCC CGGCTATCCA GGCACATACC CAAACAACAC GTACTGCGAG
TGGGTCCTTG TTGCTCCTGC TGGAAGGCTT GTCACCATCA ACTTCTACTT CATCAGCATT
GACGATCCAG GAGACTGTGT CCAGAACTAT CTCACACTCT ATGATGGGCC CAACGCCAGC
TCTCCATCCT CTGGACCATA CTGCGGAGGC GACACCAGCA TAGCTCCCTT CGTGGCTTCC
TCAAATCAGG TCTTCATAAA ATTTCATGCT GATTATGCAC GGCGTCCATC CGCATTCCGA
TTAACTTGGG ACAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518011.1
CDS227..7084
Misc Feature(1)227..376(+)
Misc Feature(2)284..376(+)
Misc Feature(3)383..727(+)
Misc Feature(4)413..727(+)
Misc Feature(5)740..1063(+)
Misc Feature(6)764..1063(+)
Misc Feature(7)1070..1411(+)
Misc Feature(8)1097..1411(+)
Misc Feature(9)1424..1753(+)
Misc Feature(10)1448..1750(+)
Misc Feature(11)1787..2098(+)
Misc Feature(12)1790..2098(+)
Misc Feature(13)2144..2476(+)
Misc Feature(14)2174..2473(+)
Misc Feature(15)2486..2848(+)
Misc Feature(16)2516..2848(+)
Misc Feature(17)2861..3211(+)
Misc Feature(18)2897..3211(+)
Misc Feature(19)3218..3553(+)
Misc Feature(20)3242..3553(+)
Misc Feature(21)3566..3904(+)
Misc Feature(22)3596..3904(+)
Misc Feature(23)3920..4270(+)
Misc Feature(24)3947..4270(+)
Misc Feature(25)4277..4612(+)
Misc Feature(26)4301..4612(+)
Misc Feature(27)4625..4966(+)
Misc Feature(28)4649..4966(+)
Misc Feature(29)4970..5314(+)
Misc Feature(30)4994..5314(+)
Misc Feature(31)5321..5656(+)
Misc Feature(32)5345..5656(+)
Misc Feature(33)5681..6031(+)
Misc Feature(34)5708..6031(+)
Misc Feature(35)6044..6388(+)
Misc Feature(36)6074..6388(+)
Misc Feature(37)6395..6730(+)
Misc Feature(38)6419..6730(+)
Misc Feature(39)6743..7075(+)
Misc Feature(40)6773..7072(+)
Translation

Target ORF information:

RefSeq Version XM_011519709
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cubilin (intrinsic factor-cobalamin receptor) (CUBN), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011519709

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
ATGCTCTATG ATGGACCACG GCAGATGGGA CGCTACTGTG GAGTAGACCT GCCCCCTCCA 
GGGAGTACTA CAAGCTCCAA GCTTCAAGTG CTGCTCCTTA CAGATGGGGT TGGCCGCCGT
GAGAAAGGAT TTCAGATGCA GTGGTTTGTT TACGGTTGTG GTGGAGAGCT GTCTGGGGCC
ACAGGCTCCT TCAGCAGCCC CGGGTTCCCC AACAGGTATC CACCAAACAA GGAGTGTATC
TGGTACATTA GGACGGACCC CGGGAGTAGC ATTCAGCTCA CCATCCATGA CTTCGATGTG
GAGTATCATT CAAGGTGCAA CTTTGATGTC TTGGAGATCT ATGGAGGCCC CGATTTCCAC
TCTCCCAGAA TAGCCCAACT GTGTACCCAG AGATCACCTG AGAACCCCAT GCAGGTCTCC
AGCACTGGAA ATGAGCTAGC AATTCGATTC AAGACCGACT TGTCCATAAA TGGGAGAGGC
TTCAATGCGT CATGGCAAGC AGTCACTGGA GGTTGTGGTG GGATTTTCCA GGCTCCCAGT
GGAGAGATTC ATTCTCCAAA TTACCCCAGT CCTTATAGGA GCAACACAGA CTGTTCTTGG
GTCATTCGGG TTGACAGAAA TCATCGTGTT CTCTTGAACT TCACTGACTT TGATCTTGAA
CCACAAGACT CTTGTATTAT GGCATACGAT GGCTTAAGCT CCACAATGTC CCGCCTTGCC
AGGACGTGTG GAAGGGAGCA GCTGGCTAAC CCCATCGTCT CCTCAGGAAA CAGCCTCTTC
TTGAGATTTC AGTCTGGCCC TTCCAGACAG AACAGAGGCT TCCGAGCTCA ATTCAGGCAA
GCCTGCGGAG GCCACATCCT CACCAGCTCA TTTGATACTG TTTCCTCTCC ACGGTTCCCT
GCCAATTATC CAAACAATCA GAACTGCAGC TGGATCATTC AAGCGCAACC TCCATTAAAT
CATATCACCC TCTCTTTTAC CCACTTTGAA CTTGAAAGAA GCACAACGTG TGCACGTGAC
TTTGTAGAAA TTTTGGATGG CGGCCACGAA GACGCGCCCC TCCGAGGCCG TTACTGTGGC
ACCGACATGC CCCATCCTAT CACATCCTTC AGCAGCGCCC TGACGCTGAG ATTCGTCTCT
GATTCTAGCA TCAGTGCTGG GGGTTTCCAC ACCACGGTCA CCGCATCAGT GTCGGCTTGT
GGTGGAACGT TCTACATGGC TGAAGGCATC TTCAACAGCC CTGGCTACCC AGACATTTAT
CCCCCTAATG TGGAATGTGT CTGGAACATC GTCAGTTCCC CTGGCAACCG GCTCCAGCTG
TCTTTTATAT CTTTCCAGTT GGAAGACTCT CAGGACTGCA GCAGAGATTT TGTGGAGATC
CGTGAAGGAA ATGCCACGGG TCACTTGGTG GGACGATACT GTGGAAACTC CTTCCCTCTC
AATTATTCTT CCATCGTTGG ACATACCCTG TGGGTCAGAT TTATCTCAGA TGGTTCTGGC
AGCGGCACGG GCTTCCAGGC CACATTTATG AAGATATTTG GCAATGATAA TATTGTGGGA
ACTCATGGGA AAGTCGCCTC TCCTTTCTGG CCTGAAAACT ACCCACATAA CTCCAATTAC
CAATGGACAG TAAATGTGAA TGCATCTCAC GTTGTCCATG GTAGAATCTT GGAGATGGAC
ATAGAAGAAA TACAAAACTG CTATTATGAC AAATTAAGGA TCTATGATGG GCCTAGCATT
CACGCCCGCC TAATTGGAGC TTACTGTGGT ACCCAGACTG AATCTTTCAG CTCCACTGGA
AATTCTTTGA CATTTCATTT TTACTCCGAC TCTTCAATCT CAGGGAAGGG ATTCCTTCTG
GAGTGGTTTG CAGTGGATGC ACCTGATGGT GTTTTACCTA CCATTGCTCC AGGTGCTTGT
GGTGGCTTCC TGAGGACGGG AGATGCACCC GTGTTTCTCT TCTCCCCGGG CTGGCCTGAC
AGTTACAGTA ATAGAGTGGA CTGTACGTGG CTCATCCAGG CTCCCGACTC TACCGTGGAA
CTCAACATTC TTTCCCTGGA CATTGAATCT CACCGAACGT GTGCCTATGA TAGCCTTGTG
ATACGAGATG GAGATAATAA CTTGGCCCAG CAGCTAGCAG TTCTCTGTGG CAGAGAGATC
CCTGGGCCCA TCCGGTCTAC TGGAGAGTAC ATGTTCATCC GCTTCACCTC GGACTCCAGT
GTAACCAGGG CAGGCTTCAA TGCATCCTTT CACAAGAGCT GCGGTGGATA TTTGCATGCA
GACAGAGGGA TCATCACGTC CCCCAAGTAT CCAGAGACTT ACCCATCCAA CCTCAACTGT
TCTTGGCACG TCCTGGTCCA AAGTGGCCTG ACCATTGCTG TCCATTTTGA ACAGCCTTTC
CAGATTCCAA ATGGAGATTC TTCTTGCAAC CAGGGGGATT ACTTGGTGCT AAGAAATGGT
CCTGATATCT GTTCTCCACC CTTGGGACCC CCTGGAGGAA ATGGTCATTT TTGTGGCAGT
CATGCTTCAT CAACTCTGTT CACCTCGGAT AATCAAATGT TTGTTCAGTT TATTTCTGAT
CACAGTAATG AAGGGCAAGG ATTTAAAATC AAATATGAGG CAAAGAGTTT AGCCTGTGGG
GGCAACGTCT ACATCCATGA TGCTGATTCT GCTGGGTATG TGACCTCCCC CAACCACCCT
CATAATTATC CCCCGCACGC TGATTGCATT TGGATCTTAG CGGCTCCACC GGAAACACGC
ATACAGCTGC AATTTGAAGA TCGATTCGAT ATTGAAGTAA CACCCAACTG TACTTCCAAC
TACCTTGAGT TGCGGGATGG AGTGGATTCG GATGCACCAA TACTTTCCAA ATTTTGTGGG
ACATCTTTGC CCAGCAGTCA GTGGTCCTCA GGAGAGGTTA TGTATTTGAG ATTTCGATCT
GACAACAGCC CCACACATGT GGGATTCAAG GCCAAGTATT CTATAGCTCA GTGTGGGGGA
AGAGTACCAG GGCAAAGTGG TGTTGTTGAA AGCATTGGAC ATCCAACACT TCCATACAGA
GACAACTTAT TCTGTGAGTG GCATCTCCAG GGGCTCTCTG GACACTATCT CACCATCTCT
TTTGAAGACT TTAACCTTCA GAATTCTTCT GGCTGTGAAA AAGACTTCGT GGAGATCTGG
GACAATCATA CCTCTGGAAA CATCTTGGGC AGATACTGTG GAAACACCAT TCCTGACAGC
ATAGACACTT CTAGCAATAC TGCTGTGGTC AGGTTTGTCA CAGACGGCTC TGTGACTGCC
TCAGGATTCA GACTGCGATT TGAATCCAGT ATGGAAGAGT GTGGTGGGGA TCTTCAGGGC
TCTATTGGAA CATTTACTTC TCCCAACTAC CCGAACCCAA ATCCTCATGG CCGGATCTGC
GAGTGGAGAA TCACTGCCCC GGAGGGAAGG CGGATCACCC TAATGTTTAA CAACCTGAGG
CTGGCCACGC ATCCGTCCTG CAACAATGAG CATGTGATAG TATTCAATGG CATTAGAAGT
AACTCACCCC AGCTAGAGAA ACTGTGTAGT AGTGTGAATG TAAGCAATGA GATTAAATCT
TCAGGAAACA CAATGAAAGT CATTTTTTTC ACGGATGGAT CCAGGCCATA TGGCGGCTTC
ACTGCTTCCT ATACCTCCAG TGAAGATGCA GTGTGTGGTG GGTCTCTTCC AAATACTCCT
GAAGGAAACT TTACTTCTCC TGGCTATGAC GGAGTCAGGA ATTACTCAAG AAACCTGAAC
TGCGAATGGA CTCTCAGCAA TCCAAATCAG GGAAATTCAT CCATTTCCAT TCACTTTGAA
GATTTTTACC TAGAAAGTCA CCAAGACTGT CAATTTGATG TCCTCGAGTT TCGAGTGGGT
GATGCTGATG GGCCCCTGAT GTGGAGACTT TGTGGTCCTT CAAAGCCTAC ATTGCCATTG
GTTATACCTT ATTCTCAGGT ATGGATTCAC TTTGTCACCA ACGAACGTGT AGAACACATT
GGATTCCATG CAAAGTATTC CTTTACAGAT TGTGGCGGAA TACAGATAGG TGACAGTGGA
GTGATCACAA GCCCCAACTA TCCAAATGCT TATGACAGCC TGACCCACTG CTCTTCGCTG
TTGGAGGCCC CACAAGGGCA CACCATCACT CTCACATTTA GTGACTTTGA TATTGAACCC
CATACAACTT GTGCTTGGGA CTCTGTCACT GTCAGGAATG GTGGGTCCCC TGAATCACCC
ATCATAGGAC AATACTGTGG AAATTCAAAC CCCAGGACAA TACAGTCAGG TTCCAATCAG
CTGGTCGTGA CTTTTAACTC AGACCATTCA TTGCAAGGTG GTGGATTTTA TGCTACGTGG
AACACACAAA CTTTAGGTTG TGGTGGAATA TTTCATTCTG ATAATGGTAC AATCAGATCC
CCTCACTGGC CTCAGAATTT TCCCGAAAAC AGCAGATGTT CCTGGACGGC CATTACTCAC
AAAAGTAAAC ACTTGGAGAT CAGCTTTGAC AACAACTTCC TAATCCCCAG CGGTGATGGA
CAATGTCAGA ATAGCTTCGT GAAGGTGTGG GCAGGAACTG AGGAGGTGGA CAAAGCCCTG
CTAGCCACTG GCTGTGGGAA CGTGGCTCCG GGTCCCGTTA TCACACCAAG TAACACATTC
ACTGCCGTCT TCCAGTCTCA GGAGGCACCA GCTCAGGGCT TCTCCGCGTC CTTTGTTAGC
CGATGTGGAA GTAATTTCAC TGGCCCTTCA GGTTACATCA TTTCTCCAAA TTACCCAAAA
CAATATGACA ACAACATGAA TTGCACCTAT GTCATAGAGG CTAATCCTCT GTCAGTGGTC
CTCTTGACTT TTGTGTCCTT CCACTTAGAA GCTCGTTCCG CTGTGACGGG AAGCTGTGTC
AACGATGGCG TGCACATTAT CAGAGGTTAC AGCGTCATGT CCACCCCATT TGCTACTGTG
TGTGGGGATG AGATGCCAGC TCCCCTCACC ATCGCTGGGC CGGTTCTGCT TAACTTCTAC
TCCAACGAGC AAATCACAGA CTTCGGATTC AAGTTTTCCT ATAGGATAAT CTCCTGTGGT
GGTGTGTTCA ATTTCTCTTC TGGAATCATC ACAAGTCCTG CCTATTCATA CGCAGACTAC
CCAAATGATA TGCACTGTCT GTATACCATC ACCGTTAGTG ACGACAAGGT GATCGAGCTC
AAGTTCAGTG ATTTTGATGT GGTTCCCTCC ACCTCCTGCT CCCATGACTA CCTGGCAATT
TACGATGGTG CCAATACCAG CGATCCCCTT CTTGGCAAAT TCTGCGGTTC CAAGCGCCCA
CCAAATGTGA AGAGCAGCAA TAATAGTATG CTCCTGGTGT TCAAGACAGA TTCATTTCAG
ACAGCAAAAG GCTGGAAGAT GTCTTTCCGG CAGACATTGG GGCCTCAGCA AGGATGTGGT
GGTTATCTGA CAGGCTCGAA TAATACCTTT GCCTCTCCTG ATTCTGATTC GAATGGAATG
TATGACAAGA ATTTAAACTG TGTATGGATC ATAATTGCAC CTGTAAACAA AGTAATTCAC
CTCACCTTCA ATACATTTGC TCTGGAGGCA GCAAGTACTA GGCAAAGATG CCTTTATGAT
TATGTAAAGT TATATGATGG GGATAGTGAA AATGCGAACT TGGCTGGAAC GTTTTGTGGT
TCCACAGTAC CTGCTCCTTT TATCTCTTCT GGTAACTTCC TTACGGTTCA ATTCATCAGT
GACTTAACAT TAGAGAGGGA AGGATTTAAT GCTACATACA CCATCATGGA CATGCCTTGT
GGTGGAACAT ACAATGCAAC TTGGACCCCA CAAAATATTT CATCACCCAA TTCATCAGAC
CCAGATGTCC CATTTTCCAT CTGTACTTGG GTCATTGATT CCCCTCCGCA TCAGCAGGTC
AAGATAACTG TGTGGGCATT ACAGCTGACC TCGCAAGACT GCACGCAGAA TTACTTACAG
CTTCAGGACT CACCGCAGGG TCACGGAAAT TCAAGATTTC AGTTCTGTGG CAGAAATGCT
TCGGCTGTGC CAGTGTTTTA TTCTTCTATG AGTACTGCAA TGGTCATTTT CAAATCTGGA
GTTGTAAACA GAAACTCTAG AATGAGTTTC ACCTATCAGA TTGCAGATTG CAACAGAGAC
TATCACAAGG CATTTGGCAA CCTGAGAAGC CCTGGATGGC CAGATAACTA CGACAATGAC
AAGGATTGCA CCGTTACTCT CACAGCCCCC CAGAACCACA CCATTTCCCT CTTTTTTCAT
TCACTTGGCA TCGAGAACTC AGTTGAATGC AGAAACGATT TCTTGGAGGT GAGAAATGGA
AGTAACAGCA ATTCACCATT ACTGGGCAAG TACTGTGGAA CTCTGCTGCC AAACCCTGTC
TTCTCTCAAA ATAATGAACT ATACCTACGA TTTAAGAGTG ATAGTGTAAC TTCTGATCGT
GGATATGAAA TCATCTGGAC TTCATCACCC TCTGGATGTG GTGGAACTCT TTATGGAGAC
AGAGGCTCAT TCACCAGCCC CGGCTATCCA GGCACATACC CAAACAACAC GTACTGCGAG
TGGGTCCTTG TTGCTCCTGC TGGAAGGCTT GTCACCATCA ACTTCTACTT CATCAGCATT
GACGATCCAG GAGACTGTGT CCAGAACTAT CTCACACTCT ATGATGGGCC CAACGCCAGC
TCTCCATCCT CTGGACCATA CTGCGGAGGC GACACCAGCA TAGCTCCCTT CGTGGCTTCC
TCAAATCAGG TCTTCATAAA ATTTCATGCT GATTATGCAC GGCGTCCATC CGCATTCCGA
TTAACTTGGG ACAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61601
Clone ID Related Accession (Same CDS sequence) XM_011519710
Accession Version XM_011519710.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6834bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cubilin isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008705.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
ATGGGACGCT ACTGTGGAGT AGACCTGCCC CCTCCAGGGA GTACTACAAG CTCCAAGCTT 
CAAGTGCTGC TCCTTACAGA TGGGGTTGGC CGCCGTGAGA AAGGATTTCA GATGCAGTGG
TTTGTTTACG GTTGTGGTGG AGAGCTGTCT GGGGCCACAG GCTCCTTCAG CAGCCCCGGG
TTCCCCAACA GGTATCCACC AAACAAGGAG TGTATCTGGT ACATTAGGAC GGACCCCGGG
AGTAGCATTC AGCTCACCAT CCATGACTTC GATGTGGAGT ATCATTCAAG GTGCAACTTT
GATGTCTTGG AGATCTATGG AGGCCCCGAT TTCCACTCTC CCAGAATAGC CCAACTGTGT
ACCCAGAGAT CACCTGAGAA CCCCATGCAG GTCTCCAGCA CTGGAAATGA GCTAGCAATT
CGATTCAAGA CCGACTTGTC CATAAATGGG AGAGGCTTCA ATGCGTCATG GCAAGCAGTC
ACTGGAGGTT GTGGTGGGAT TTTCCAGGCT CCCAGTGGAG AGATTCATTC TCCAAATTAC
CCCAGTCCTT ATAGGAGCAA CACAGACTGT TCTTGGGTCA TTCGGGTTGA CAGAAATCAT
CGTGTTCTCT TGAACTTCAC TGACTTTGAT CTTGAACCAC AAGACTCTTG TATTATGGCA
TACGATGGCT TAAGCTCCAC AATGTCCCGC CTTGCCAGGA CGTGTGGAAG GGAGCAGCTG
GCTAACCCCA TCGTCTCCTC AGGAAACAGC CTCTTCTTGA GATTTCAGTC TGGCCCTTCC
AGACAGAACA GAGGCTTCCG AGCTCAATTC AGGCAAGCCT GCGGAGGCCA CATCCTCACC
AGCTCATTTG ATACTGTTTC CTCTCCACGG TTCCCTGCCA ATTATCCAAA CAATCAGAAC
TGCAGCTGGA TCATTCAAGC GCAACCTCCA TTAAATCATA TCACCCTCTC TTTTACCCAC
TTTGAACTTG AAAGAAGCAC AACGTGTGCA CGTGACTTTG TAGAAATTTT GGATGGCGGC
CACGAAGACG CGCCCCTCCG AGGCCGTTAC TGTGGCACCG ACATGCCCCA TCCTATCACA
TCCTTCAGCA GCGCCCTGAC GCTGAGATTC GTCTCTGATT CTAGCATCAG TGCTGGGGGT
TTCCACACCA CGGTCACCGC ATCAGTGTCG GCTTGTGGTG GAACGTTCTA CATGGCTGAA
GGCATCTTCA ACAGCCCTGG CTACCCAGAC ATTTATCCCC CTAATGTGGA ATGTGTCTGG
AACATCGTCA GTTCCCCTGG CAACCGGCTC CAGCTGTCTT TTATATCTTT CCAGTTGGAA
GACTCTCAGG ACTGCAGCAG AGATTTTGTG GAGATCCGTG AAGGAAATGC CACGGGTCAC
TTGGTGGGAC GATACTGTGG AAACTCCTTC CCTCTCAATT ATTCTTCCAT CGTTGGACAT
ACCCTGTGGG TCAGATTTAT CTCAGATGGT TCTGGCAGCG GCACGGGCTT CCAGGCCACA
TTTATGAAGA TATTTGGCAA TGATAATATT GTGGGAACTC ATGGGAAAGT CGCCTCTCCT
TTCTGGCCTG AAAACTACCC ACATAACTCC AATTACCAAT GGACAGTAAA TGTGAATGCA
TCTCACGTTG TCCATGGTAG AATCTTGGAG ATGGACATAG AAGAAATACA AAACTGCTAT
TATGACAAAT TAAGGATCTA TGATGGGCCT AGCATTCACG CCCGCCTAAT TGGAGCTTAC
TGTGGTACCC AGACTGAATC TTTCAGCTCC ACTGGAAATT CTTTGACATT TCATTTTTAC
TCCGACTCTT CAATCTCAGG GAAGGGATTC CTTCTGGAGT GGTTTGCAGT GGATGCACCT
GATGGTGTTT TACCTACCAT TGCTCCAGGT GCTTGTGGTG GCTTCCTGAG GACGGGAGAT
GCACCCGTGT TTCTCTTCTC CCCGGGCTGG CCTGACAGTT ACAGTAATAG AGTGGACTGT
ACGTGGCTCA TCCAGGCTCC CGACTCTACC GTGGAACTCA ACATTCTTTC CCTGGACATT
GAATCTCACC GAACGTGTGC CTATGATAGC CTTGTGATAC GAGATGGAGA TAATAACTTG
GCCCAGCAGC TAGCAGTTCT CTGTGGCAGA GAGATCCCTG GGCCCATCCG GTCTACTGGA
GAGTACATGT TCATCCGCTT CACCTCGGAC TCCAGTGTAA CCAGGGCAGG CTTCAATGCA
TCCTTTCACA AGAGCTGCGG TGGATATTTG CATGCAGACA GAGGGATCAT CACGTCCCCC
AAGTATCCAG AGACTTACCC ATCCAACCTC AACTGTTCTT GGCACGTCCT GGTCCAAAGT
GGCCTGACCA TTGCTGTCCA TTTTGAACAG CCTTTCCAGA TTCCAAATGG AGATTCTTCT
TGCAACCAGG GGGATTACTT GGTGCTAAGA AATGGTCCTG ATATCTGTTC TCCACCCTTG
GGACCCCCTG GAGGAAATGG TCATTTTTGT GGCAGTCATG CTTCATCAAC TCTGTTCACC
TCGGATAATC AAATGTTTGT TCAGTTTATT TCTGATCACA GTAATGAAGG GCAAGGATTT
AAAATCAAAT ATGAGGCAAA GAGTTTAGCC TGTGGGGGCA ACGTCTACAT CCATGATGCT
GATTCTGCTG GGTATGTGAC CTCCCCCAAC CACCCTCATA ATTATCCCCC GCACGCTGAT
TGCATTTGGA TCTTAGCGGC TCCACCGGAA ACACGCATAC AGCTGCAATT TGAAGATCGA
TTCGATATTG AAGTAACACC CAACTGTACT TCCAACTACC TTGAGTTGCG GGATGGAGTG
GATTCGGATG CACCAATACT TTCCAAATTT TGTGGGACAT CTTTGCCCAG CAGTCAGTGG
TCCTCAGGAG AGGTTATGTA TTTGAGATTT CGATCTGACA ACAGCCCCAC ACATGTGGGA
TTCAAGGCCA AGTATTCTAT AGCTCAGTGT GGGGGAAGAG TACCAGGGCA AAGTGGTGTT
GTTGAAAGCA TTGGACATCC AACACTTCCA TACAGAGACA ACTTATTCTG TGAGTGGCAT
CTCCAGGGGC TCTCTGGACA CTATCTCACC ATCTCTTTTG AAGACTTTAA CCTTCAGAAT
TCTTCTGGCT GTGAAAAAGA CTTCGTGGAG ATCTGGGACA ATCATACCTC TGGAAACATC
TTGGGCAGAT ACTGTGGAAA CACCATTCCT GACAGCATAG ACACTTCTAG CAATACTGCT
GTGGTCAGGT TTGTCACAGA CGGCTCTGTG ACTGCCTCAG GATTCAGACT GCGATTTGAA
TCCAGTATGG AAGAGTGTGG TGGGGATCTT CAGGGCTCTA TTGGAACATT TACTTCTCCC
AACTACCCGA ACCCAAATCC TCATGGCCGG ATCTGCGAGT GGAGAATCAC TGCCCCGGAG
GGAAGGCGGA TCACCCTAAT GTTTAACAAC CTGAGGCTGG CCACGCATCC GTCCTGCAAC
AATGAGCATG TGATAGTATT CAATGGCATT AGAAGTAACT CACCCCAGCT AGAGAAACTG
TGTAGTAGTG TGAATGTAAG CAATGAGATT AAATCTTCAG GAAACACAAT GAAAGTCATT
TTTTTCACGG ATGGATCCAG GCCATATGGC GGCTTCACTG CTTCCTATAC CTCCAGTGAA
GATGCAGTGT GTGGTGGGTC TCTTCCAAAT ACTCCTGAAG GAAACTTTAC TTCTCCTGGC
TATGACGGAG TCAGGAATTA CTCAAGAAAC CTGAACTGCG AATGGACTCT CAGCAATCCA
AATCAGGGAA ATTCATCCAT TTCCATTCAC TTTGAAGATT TTTACCTAGA AAGTCACCAA
GACTGTCAAT TTGATGTCCT CGAGTTTCGA GTGGGTGATG CTGATGGGCC CCTGATGTGG
AGACTTTGTG GTCCTTCAAA GCCTACATTG CCATTGGTTA TACCTTATTC TCAGGTATGG
ATTCACTTTG TCACCAACGA ACGTGTAGAA CACATTGGAT TCCATGCAAA GTATTCCTTT
ACAGATTGTG GCGGAATACA GATAGGTGAC AGTGGAGTGA TCACAAGCCC CAACTATCCA
AATGCTTATG ACAGCCTGAC CCACTGCTCT TCGCTGTTGG AGGCCCCACA AGGGCACACC
ATCACTCTCA CATTTAGTGA CTTTGATATT GAACCCCATA CAACTTGTGC TTGGGACTCT
GTCACTGTCA GGAATGGTGG GTCCCCTGAA TCACCCATCA TAGGACAATA CTGTGGAAAT
TCAAACCCCA GGACAATACA GTCAGGTTCC AATCAGCTGG TCGTGACTTT TAACTCAGAC
CATTCATTGC AAGGTGGTGG ATTTTATGCT ACGTGGAACA CACAAACTTT AGGTTGTGGT
GGAATATTTC ATTCTGATAA TGGTACAATC AGATCCCCTC ACTGGCCTCA GAATTTTCCC
GAAAACAGCA GATGTTCCTG GACGGCCATT ACTCACAAAA GTAAACACTT GGAGATCAGC
TTTGACAACA ACTTCCTAAT CCCCAGCGGT GATGGACAAT GTCAGAATAG CTTCGTGAAG
GTGTGGGCAG GAACTGAGGA GGTGGACAAA GCCCTGCTAG CCACTGGCTG TGGGAACGTG
GCTCCGGGTC CCGTTATCAC ACCAAGTAAC ACATTCACTG CCGTCTTCCA GTCTCAGGAG
GCACCAGCTC AGGGCTTCTC CGCGTCCTTT GTTAGCCGAT GTGGAAGTAA TTTCACTGGC
CCTTCAGGTT ACATCATTTC TCCAAATTAC CCAAAACAAT ATGACAACAA CATGAATTGC
ACCTATGTCA TAGAGGCTAA TCCTCTGTCA GTGGTCCTCT TGACTTTTGT GTCCTTCCAC
TTAGAAGCTC GTTCCGCTGT GACGGGAAGC TGTGTCAACG ATGGCGTGCA CATTATCAGA
GGTTACAGCG TCATGTCCAC CCCATTTGCT ACTGTGTGTG GGGATGAGAT GCCAGCTCCC
CTCACCATCG CTGGGCCGGT TCTGCTTAAC TTCTACTCCA ACGAGCAAAT CACAGACTTC
GGATTCAAGT TTTCCTATAG GATAATCTCC TGTGGTGGTG TGTTCAATTT CTCTTCTGGA
ATCATCACAA GTCCTGCCTA TTCATACGCA GACTACCCAA ATGATATGCA CTGTCTGTAT
ACCATCACCG TTAGTGACGA CAAGGTGATC GAGCTCAAGT TCAGTGATTT TGATGTGGTT
CCCTCCACCT CCTGCTCCCA TGACTACCTG GCAATTTACG ATGGTGCCAA TACCAGCGAT
CCCCTTCTTG GCAAATTCTG CGGTTCCAAG CGCCCACCAA ATGTGAAGAG CAGCAATAAT
AGTATGCTCC TGGTGTTCAA GACAGATTCA TTTCAGACAG CAAAAGGCTG GAAGATGTCT
TTCCGGCAGA CATTGGGGCC TCAGCAAGGA TGTGGTGGTT ATCTGACAGG CTCGAATAAT
ACCTTTGCCT CTCCTGATTC TGATTCGAAT GGAATGTATG ACAAGAATTT AAACTGTGTA
TGGATCATAA TTGCACCTGT AAACAAAGTA ATTCACCTCA CCTTCAATAC ATTTGCTCTG
GAGGCAGCAA GTACTAGGCA AAGATGCCTT TATGATTATG TAAAGTTATA TGATGGGGAT
AGTGAAAATG CGAACTTGGC TGGAACGTTT TGTGGTTCCA CAGTACCTGC TCCTTTTATC
TCTTCTGGTA ACTTCCTTAC GGTTCAATTC ATCAGTGACT TAACATTAGA GAGGGAAGGA
TTTAATGCTA CATACACCAT CATGGACATG CCTTGTGGTG GAACATACAA TGCAACTTGG
ACCCCACAAA ATATTTCATC ACCCAATTCA TCAGACCCAG ATGTCCCATT TTCCATCTGT
ACTTGGGTCA TTGATTCCCC TCCGCATCAG CAGGTCAAGA TAACTGTGTG GGCATTACAG
CTGACCTCGC AAGACTGCAC GCAGAATTAC TTACAGCTTC AGGACTCACC GCAGGGTCAC
GGAAATTCAA GATTTCAGTT CTGTGGCAGA AATGCTTCGG CTGTGCCAGT GTTTTATTCT
TCTATGAGTA CTGCAATGGT CATTTTCAAA TCTGGAGTTG TAAACAGAAA CTCTAGAATG
AGTTTCACCT ATCAGATTGC AGATTGCAAC AGAGACTATC ACAAGGCATT TGGCAACCTG
AGAAGCCCTG GATGGCCAGA TAACTACGAC AATGACAAGG ATTGCACCGT TACTCTCACA
GCCCCCCAGA ACCACACCAT TTCCCTCTTT TTTCATTCAC TTGGCATCGA GAACTCAGTT
GAATGCAGAA ACGATTTCTT GGAGGTGAGA AATGGAAGTA ACAGCAATTC ACCATTACTG
GGCAAGTACT GTGGAACTCT GCTGCCAAAC CCTGTCTTCT CTCAAAATAA TGAACTATAC
CTACGATTTA AGAGTGATAG TGTAACTTCT GATCGTGGAT ATGAAATCAT CTGGACTTCA
TCACCCTCTG GATGTGGTGG AACTCTTTAT GGAGACAGAG GCTCATTCAC CAGCCCCGGC
TATCCAGGCA CATACCCAAA CAACACGTAC TGCGAGTGGG TCCTTGTTGC TCCTGCTGGA
AGGCTTGTCA CCATCAACTT CTACTTCATC AGCATTGACG ATCCAGGAGA CTGTGTCCAG
AACTATCTCA CACTCTATGA TGGGCCCAAC GCCAGCTCTC CATCCTCTGG ACCATACTGC
GGAGGCGACA CCAGCATAGC TCCCTTCGTG GCTTCCTCAA ATCAGGTCTT CATAAAATTT
CATGCTGATT ATGCACGGCG TCCATCCGCA TTCCGATTAA CTTGGGACAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518012.1
CDS89..6922
Misc Feature(1)221..565(+)
Misc Feature(2)251..565(+)
Misc Feature(3)578..901(+)
Misc Feature(4)602..901(+)
Misc Feature(5)908..1249(+)
Misc Feature(6)935..1249(+)
Misc Feature(7)1262..1591(+)
Misc Feature(8)1286..1588(+)
Misc Feature(9)1625..1936(+)
Misc Feature(10)1628..1936(+)
Misc Feature(11)1982..2314(+)
Misc Feature(12)2012..2311(+)
Misc Feature(13)2324..2686(+)
Misc Feature(14)2354..2686(+)
Misc Feature(15)2699..3049(+)
Misc Feature(16)2735..3049(+)
Misc Feature(17)3056..3391(+)
Misc Feature(18)3080..3391(+)
Misc Feature(19)3404..3742(+)
Misc Feature(20)3434..3742(+)
Misc Feature(21)3758..4108(+)
Misc Feature(22)3785..4108(+)
Misc Feature(23)4115..4450(+)
Misc Feature(24)4139..4450(+)
Misc Feature(25)4463..4804(+)
Misc Feature(26)4487..4804(+)
Misc Feature(27)4808..5152(+)
Misc Feature(28)4832..5152(+)
Misc Feature(29)5159..5494(+)
Misc Feature(30)5183..5494(+)
Misc Feature(31)5519..5869(+)
Misc Feature(32)5546..5869(+)
Misc Feature(33)5882..6226(+)
Misc Feature(34)5912..6226(+)
Misc Feature(35)6233..6568(+)
Misc Feature(36)6257..6568(+)
Misc Feature(37)6581..6913(+)
Misc Feature(38)6611..6910(+)
Translation

Target ORF information:

RefSeq Version XM_011519710
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cubilin (intrinsic factor-cobalamin receptor) (CUBN), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011519710

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
ATGGGACGCT ACTGTGGAGT AGACCTGCCC CCTCCAGGGA GTACTACAAG CTCCAAGCTT 
CAAGTGCTGC TCCTTACAGA TGGGGTTGGC CGCCGTGAGA AAGGATTTCA GATGCAGTGG
TTTGTTTACG GTTGTGGTGG AGAGCTGTCT GGGGCCACAG GCTCCTTCAG CAGCCCCGGG
TTCCCCAACA GGTATCCACC AAACAAGGAG TGTATCTGGT ACATTAGGAC GGACCCCGGG
AGTAGCATTC AGCTCACCAT CCATGACTTC GATGTGGAGT ATCATTCAAG GTGCAACTTT
GATGTCTTGG AGATCTATGG AGGCCCCGAT TTCCACTCTC CCAGAATAGC CCAACTGTGT
ACCCAGAGAT CACCTGAGAA CCCCATGCAG GTCTCCAGCA CTGGAAATGA GCTAGCAATT
CGATTCAAGA CCGACTTGTC CATAAATGGG AGAGGCTTCA ATGCGTCATG GCAAGCAGTC
ACTGGAGGTT GTGGTGGGAT TTTCCAGGCT CCCAGTGGAG AGATTCATTC TCCAAATTAC
CCCAGTCCTT ATAGGAGCAA CACAGACTGT TCTTGGGTCA TTCGGGTTGA CAGAAATCAT
CGTGTTCTCT TGAACTTCAC TGACTTTGAT CTTGAACCAC AAGACTCTTG TATTATGGCA
TACGATGGCT TAAGCTCCAC AATGTCCCGC CTTGCCAGGA CGTGTGGAAG GGAGCAGCTG
GCTAACCCCA TCGTCTCCTC AGGAAACAGC CTCTTCTTGA GATTTCAGTC TGGCCCTTCC
AGACAGAACA GAGGCTTCCG AGCTCAATTC AGGCAAGCCT GCGGAGGCCA CATCCTCACC
AGCTCATTTG ATACTGTTTC CTCTCCACGG TTCCCTGCCA ATTATCCAAA CAATCAGAAC
TGCAGCTGGA TCATTCAAGC GCAACCTCCA TTAAATCATA TCACCCTCTC TTTTACCCAC
TTTGAACTTG AAAGAAGCAC AACGTGTGCA CGTGACTTTG TAGAAATTTT GGATGGCGGC
CACGAAGACG CGCCCCTCCG AGGCCGTTAC TGTGGCACCG ACATGCCCCA TCCTATCACA
TCCTTCAGCA GCGCCCTGAC GCTGAGATTC GTCTCTGATT CTAGCATCAG TGCTGGGGGT
TTCCACACCA CGGTCACCGC ATCAGTGTCG GCTTGTGGTG GAACGTTCTA CATGGCTGAA
GGCATCTTCA ACAGCCCTGG CTACCCAGAC ATTTATCCCC CTAATGTGGA ATGTGTCTGG
AACATCGTCA GTTCCCCTGG CAACCGGCTC CAGCTGTCTT TTATATCTTT CCAGTTGGAA
GACTCTCAGG ACTGCAGCAG AGATTTTGTG GAGATCCGTG AAGGAAATGC CACGGGTCAC
TTGGTGGGAC GATACTGTGG AAACTCCTTC CCTCTCAATT ATTCTTCCAT CGTTGGACAT
ACCCTGTGGG TCAGATTTAT CTCAGATGGT TCTGGCAGCG GCACGGGCTT CCAGGCCACA
TTTATGAAGA TATTTGGCAA TGATAATATT GTGGGAACTC ATGGGAAAGT CGCCTCTCCT
TTCTGGCCTG AAAACTACCC ACATAACTCC AATTACCAAT GGACAGTAAA TGTGAATGCA
TCTCACGTTG TCCATGGTAG AATCTTGGAG ATGGACATAG AAGAAATACA AAACTGCTAT
TATGACAAAT TAAGGATCTA TGATGGGCCT AGCATTCACG CCCGCCTAAT TGGAGCTTAC
TGTGGTACCC AGACTGAATC TTTCAGCTCC ACTGGAAATT CTTTGACATT TCATTTTTAC
TCCGACTCTT CAATCTCAGG GAAGGGATTC CTTCTGGAGT GGTTTGCAGT GGATGCACCT
GATGGTGTTT TACCTACCAT TGCTCCAGGT GCTTGTGGTG GCTTCCTGAG GACGGGAGAT
GCACCCGTGT TTCTCTTCTC CCCGGGCTGG CCTGACAGTT ACAGTAATAG AGTGGACTGT
ACGTGGCTCA TCCAGGCTCC CGACTCTACC GTGGAACTCA ACATTCTTTC CCTGGACATT
GAATCTCACC GAACGTGTGC CTATGATAGC CTTGTGATAC GAGATGGAGA TAATAACTTG
GCCCAGCAGC TAGCAGTTCT CTGTGGCAGA GAGATCCCTG GGCCCATCCG GTCTACTGGA
GAGTACATGT TCATCCGCTT CACCTCGGAC TCCAGTGTAA CCAGGGCAGG CTTCAATGCA
TCCTTTCACA AGAGCTGCGG TGGATATTTG CATGCAGACA GAGGGATCAT CACGTCCCCC
AAGTATCCAG AGACTTACCC ATCCAACCTC AACTGTTCTT GGCACGTCCT GGTCCAAAGT
GGCCTGACCA TTGCTGTCCA TTTTGAACAG CCTTTCCAGA TTCCAAATGG AGATTCTTCT
TGCAACCAGG GGGATTACTT GGTGCTAAGA AATGGTCCTG ATATCTGTTC TCCACCCTTG
GGACCCCCTG GAGGAAATGG TCATTTTTGT GGCAGTCATG CTTCATCAAC TCTGTTCACC
TCGGATAATC AAATGTTTGT TCAGTTTATT TCTGATCACA GTAATGAAGG GCAAGGATTT
AAAATCAAAT ATGAGGCAAA GAGTTTAGCC TGTGGGGGCA ACGTCTACAT CCATGATGCT
GATTCTGCTG GGTATGTGAC CTCCCCCAAC CACCCTCATA ATTATCCCCC GCACGCTGAT
TGCATTTGGA TCTTAGCGGC TCCACCGGAA ACACGCATAC AGCTGCAATT TGAAGATCGA
TTCGATATTG AAGTAACACC CAACTGTACT TCCAACTACC TTGAGTTGCG GGATGGAGTG
GATTCGGATG CACCAATACT TTCCAAATTT TGTGGGACAT CTTTGCCCAG CAGTCAGTGG
TCCTCAGGAG AGGTTATGTA TTTGAGATTT CGATCTGACA ACAGCCCCAC ACATGTGGGA
TTCAAGGCCA AGTATTCTAT AGCTCAGTGT GGGGGAAGAG TACCAGGGCA AAGTGGTGTT
GTTGAAAGCA TTGGACATCC AACACTTCCA TACAGAGACA ACTTATTCTG TGAGTGGCAT
CTCCAGGGGC TCTCTGGACA CTATCTCACC ATCTCTTTTG AAGACTTTAA CCTTCAGAAT
TCTTCTGGCT GTGAAAAAGA CTTCGTGGAG ATCTGGGACA ATCATACCTC TGGAAACATC
TTGGGCAGAT ACTGTGGAAA CACCATTCCT GACAGCATAG ACACTTCTAG CAATACTGCT
GTGGTCAGGT TTGTCACAGA CGGCTCTGTG ACTGCCTCAG GATTCAGACT GCGATTTGAA
TCCAGTATGG AAGAGTGTGG TGGGGATCTT CAGGGCTCTA TTGGAACATT TACTTCTCCC
AACTACCCGA ACCCAAATCC TCATGGCCGG ATCTGCGAGT GGAGAATCAC TGCCCCGGAG
GGAAGGCGGA TCACCCTAAT GTTTAACAAC CTGAGGCTGG CCACGCATCC GTCCTGCAAC
AATGAGCATG TGATAGTATT CAATGGCATT AGAAGTAACT CACCCCAGCT AGAGAAACTG
TGTAGTAGTG TGAATGTAAG CAATGAGATT AAATCTTCAG GAAACACAAT GAAAGTCATT
TTTTTCACGG ATGGATCCAG GCCATATGGC GGCTTCACTG CTTCCTATAC CTCCAGTGAA
GATGCAGTGT GTGGTGGGTC TCTTCCAAAT ACTCCTGAAG GAAACTTTAC TTCTCCTGGC
TATGACGGAG TCAGGAATTA CTCAAGAAAC CTGAACTGCG AATGGACTCT CAGCAATCCA
AATCAGGGAA ATTCATCCAT TTCCATTCAC TTTGAAGATT TTTACCTAGA AAGTCACCAA
GACTGTCAAT TTGATGTCCT CGAGTTTCGA GTGGGTGATG CTGATGGGCC CCTGATGTGG
AGACTTTGTG GTCCTTCAAA GCCTACATTG CCATTGGTTA TACCTTATTC TCAGGTATGG
ATTCACTTTG TCACCAACGA ACGTGTAGAA CACATTGGAT TCCATGCAAA GTATTCCTTT
ACAGATTGTG GCGGAATACA GATAGGTGAC AGTGGAGTGA TCACAAGCCC CAACTATCCA
AATGCTTATG ACAGCCTGAC CCACTGCTCT TCGCTGTTGG AGGCCCCACA AGGGCACACC
ATCACTCTCA CATTTAGTGA CTTTGATATT GAACCCCATA CAACTTGTGC TTGGGACTCT
GTCACTGTCA GGAATGGTGG GTCCCCTGAA TCACCCATCA TAGGACAATA CTGTGGAAAT
TCAAACCCCA GGACAATACA GTCAGGTTCC AATCAGCTGG TCGTGACTTT TAACTCAGAC
CATTCATTGC AAGGTGGTGG ATTTTATGCT ACGTGGAACA CACAAACTTT AGGTTGTGGT
GGAATATTTC ATTCTGATAA TGGTACAATC AGATCCCCTC ACTGGCCTCA GAATTTTCCC
GAAAACAGCA GATGTTCCTG GACGGCCATT ACTCACAAAA GTAAACACTT GGAGATCAGC
TTTGACAACA ACTTCCTAAT CCCCAGCGGT GATGGACAAT GTCAGAATAG CTTCGTGAAG
GTGTGGGCAG GAACTGAGGA GGTGGACAAA GCCCTGCTAG CCACTGGCTG TGGGAACGTG
GCTCCGGGTC CCGTTATCAC ACCAAGTAAC ACATTCACTG CCGTCTTCCA GTCTCAGGAG
GCACCAGCTC AGGGCTTCTC CGCGTCCTTT GTTAGCCGAT GTGGAAGTAA TTTCACTGGC
CCTTCAGGTT ACATCATTTC TCCAAATTAC CCAAAACAAT ATGACAACAA CATGAATTGC
ACCTATGTCA TAGAGGCTAA TCCTCTGTCA GTGGTCCTCT TGACTTTTGT GTCCTTCCAC
TTAGAAGCTC GTTCCGCTGT GACGGGAAGC TGTGTCAACG ATGGCGTGCA CATTATCAGA
GGTTACAGCG TCATGTCCAC CCCATTTGCT ACTGTGTGTG GGGATGAGAT GCCAGCTCCC
CTCACCATCG CTGGGCCGGT TCTGCTTAAC TTCTACTCCA ACGAGCAAAT CACAGACTTC
GGATTCAAGT TTTCCTATAG GATAATCTCC TGTGGTGGTG TGTTCAATTT CTCTTCTGGA
ATCATCACAA GTCCTGCCTA TTCATACGCA GACTACCCAA ATGATATGCA CTGTCTGTAT
ACCATCACCG TTAGTGACGA CAAGGTGATC GAGCTCAAGT TCAGTGATTT TGATGTGGTT
CCCTCCACCT CCTGCTCCCA TGACTACCTG GCAATTTACG ATGGTGCCAA TACCAGCGAT
CCCCTTCTTG GCAAATTCTG CGGTTCCAAG CGCCCACCAA ATGTGAAGAG CAGCAATAAT
AGTATGCTCC TGGTGTTCAA GACAGATTCA TTTCAGACAG CAAAAGGCTG GAAGATGTCT
TTCCGGCAGA CATTGGGGCC TCAGCAAGGA TGTGGTGGTT ATCTGACAGG CTCGAATAAT
ACCTTTGCCT CTCCTGATTC TGATTCGAAT GGAATGTATG ACAAGAATTT AAACTGTGTA
TGGATCATAA TTGCACCTGT AAACAAAGTA ATTCACCTCA CCTTCAATAC ATTTGCTCTG
GAGGCAGCAA GTACTAGGCA AAGATGCCTT TATGATTATG TAAAGTTATA TGATGGGGAT
AGTGAAAATG CGAACTTGGC TGGAACGTTT TGTGGTTCCA CAGTACCTGC TCCTTTTATC
TCTTCTGGTA ACTTCCTTAC GGTTCAATTC ATCAGTGACT TAACATTAGA GAGGGAAGGA
TTTAATGCTA CATACACCAT CATGGACATG CCTTGTGGTG GAACATACAA TGCAACTTGG
ACCCCACAAA ATATTTCATC ACCCAATTCA TCAGACCCAG ATGTCCCATT TTCCATCTGT
ACTTGGGTCA TTGATTCCCC TCCGCATCAG CAGGTCAAGA TAACTGTGTG GGCATTACAG
CTGACCTCGC AAGACTGCAC GCAGAATTAC TTACAGCTTC AGGACTCACC GCAGGGTCAC
GGAAATTCAA GATTTCAGTT CTGTGGCAGA AATGCTTCGG CTGTGCCAGT GTTTTATTCT
TCTATGAGTA CTGCAATGGT CATTTTCAAA TCTGGAGTTG TAAACAGAAA CTCTAGAATG
AGTTTCACCT ATCAGATTGC AGATTGCAAC AGAGACTATC ACAAGGCATT TGGCAACCTG
AGAAGCCCTG GATGGCCAGA TAACTACGAC AATGACAAGG ATTGCACCGT TACTCTCACA
GCCCCCCAGA ACCACACCAT TTCCCTCTTT TTTCATTCAC TTGGCATCGA GAACTCAGTT
GAATGCAGAA ACGATTTCTT GGAGGTGAGA AATGGAAGTA ACAGCAATTC ACCATTACTG
GGCAAGTACT GTGGAACTCT GCTGCCAAAC CCTGTCTTCT CTCAAAATAA TGAACTATAC
CTACGATTTA AGAGTGATAG TGTAACTTCT GATCGTGGAT ATGAAATCAT CTGGACTTCA
TCACCCTCTG GATGTGGTGG AACTCTTTAT GGAGACAGAG GCTCATTCAC CAGCCCCGGC
TATCCAGGCA CATACCCAAA CAACACGTAC TGCGAGTGGG TCCTTGTTGC TCCTGCTGGA
AGGCTTGTCA CCATCAACTT CTACTTCATC AGCATTGACG ATCCAGGAGA CTGTGTCCAG
AACTATCTCA CACTCTATGA TGGGCCCAAC GCCAGCTCTC CATCCTCTGG ACCATACTGC
GGAGGCGACA CCAGCATAGC TCCCTTCGTG GCTTCCTCAA ATCAGGTCTT CATAAAATTT
CATGCTGATT ATGCACGGCG TCCATCCGCA TTCCGATTAA CTTGGGACAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61602
Clone ID Related Accession (Same CDS sequence) XM_011519711
Accession Version XM_011519711.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6714bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cubilin isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008705.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
ATGATCCAAG GTTGTGGTGG AGAGCTGTCT GGGGCCACAG GCTCCTTCAG CAGCCCCGGG 
TTCCCCAACA GGTATCCACC AAACAAGGAG TGTATCTGGT ACATTAGGAC GGACCCCGGG
AGTAGCATTC AGCTCACCAT CCATGACTTC GATGTGGAGT ATCATTCAAG GTGCAACTTT
GATGTCTTGG AGATCTATGG AGGCCCCGAT TTCCACTCTC CCAGAATAGC CCAACTGTGT
ACCCAGAGAT CACCTGAGAA CCCCATGCAG GTCTCCAGCA CTGGAAATGA GCTAGCAATT
CGATTCAAGA CCGACTTGTC CATAAATGGG AGAGGCTTCA ATGCGTCATG GCAAGCAGTC
ACTGGAGGTT GTGGTGGGAT TTTCCAGGCT CCCAGTGGAG AGATTCATTC TCCAAATTAC
CCCAGTCCTT ATAGGAGCAA CACAGACTGT TCTTGGGTCA TTCGGGTTGA CAGAAATCAT
CGTGTTCTCT TGAACTTCAC TGACTTTGAT CTTGAACCAC AAGACTCTTG TATTATGGCA
TACGATGGCT TAAGCTCCAC AATGTCCCGC CTTGCCAGGA CGTGTGGAAG GGAGCAGCTG
GCTAACCCCA TCGTCTCCTC AGGAAACAGC CTCTTCTTGA GATTTCAGTC TGGCCCTTCC
AGACAGAACA GAGGCTTCCG AGCTCAATTC AGGCAAGCCT GCGGAGGCCA CATCCTCACC
AGCTCATTTG ATACTGTTTC CTCTCCACGG TTCCCTGCCA ATTATCCAAA CAATCAGAAC
TGCAGCTGGA TCATTCAAGC GCAACCTCCA TTAAATCATA TCACCCTCTC TTTTACCCAC
TTTGAACTTG AAAGAAGCAC AACGTGTGCA CGTGACTTTG TAGAAATTTT GGATGGCGGC
CACGAAGACG CGCCCCTCCG AGGCCGTTAC TGTGGCACCG ACATGCCCCA TCCTATCACA
TCCTTCAGCA GCGCCCTGAC GCTGAGATTC GTCTCTGATT CTAGCATCAG TGCTGGGGGT
TTCCACACCA CGGTCACCGC ATCAGTGTCG GCTTGTGGTG GAACGTTCTA CATGGCTGAA
GGCATCTTCA ACAGCCCTGG CTACCCAGAC ATTTATCCCC CTAATGTGGA ATGTGTCTGG
AACATCGTCA GTTCCCCTGG CAACCGGCTC CAGCTGTCTT TTATATCTTT CCAGTTGGAA
GACTCTCAGG ACTGCAGCAG AGATTTTGTG GAGATCCGTG AAGGAAATGC CACGGGTCAC
TTGGTGGGAC GATACTGTGG AAACTCCTTC CCTCTCAATT ATTCTTCCAT CGTTGGACAT
ACCCTGTGGG TCAGATTTAT CTCAGATGGT TCTGGCAGCG GCACGGGCTT CCAGGCCACA
TTTATGAAGA TATTTGGCAA TGATAATATT GTGGGAACTC ATGGGAAAGT CGCCTCTCCT
TTCTGGCCTG AAAACTACCC ACATAACTCC AATTACCAAT GGACAGTAAA TGTGAATGCA
TCTCACGTTG TCCATGGTAG AATCTTGGAG ATGGACATAG AAGAAATACA AAACTGCTAT
TATGACAAAT TAAGGATCTA TGATGGGCCT AGCATTCACG CCCGCCTAAT TGGAGCTTAC
TGTGGTACCC AGACTGAATC TTTCAGCTCC ACTGGAAATT CTTTGACATT TCATTTTTAC
TCCGACTCTT CAATCTCAGG GAAGGGATTC CTTCTGGAGT GGTTTGCAGT GGATGCACCT
GATGGTGTTT TACCTACCAT TGCTCCAGGT GCTTGTGGTG GCTTCCTGAG GACGGGAGAT
GCACCCGTGT TTCTCTTCTC CCCGGGCTGG CCTGACAGTT ACAGTAATAG AGTGGACTGT
ACGTGGCTCA TCCAGGCTCC CGACTCTACC GTGGAACTCA ACATTCTTTC CCTGGACATT
GAATCTCACC GAACGTGTGC CTATGATAGC CTTGTGATAC GAGATGGAGA TAATAACTTG
GCCCAGCAGC TAGCAGTTCT CTGTGGCAGA GAGATCCCTG GGCCCATCCG GTCTACTGGA
GAGTACATGT TCATCCGCTT CACCTCGGAC TCCAGTGTAA CCAGGGCAGG CTTCAATGCA
TCCTTTCACA AGAGCTGCGG TGGATATTTG CATGCAGACA GAGGGATCAT CACGTCCCCC
AAGTATCCAG AGACTTACCC ATCCAACCTC AACTGTTCTT GGCACGTCCT GGTCCAAAGT
GGCCTGACCA TTGCTGTCCA TTTTGAACAG CCTTTCCAGA TTCCAAATGG AGATTCTTCT
TGCAACCAGG GGGATTACTT GGTGCTAAGA AATGGTCCTG ATATCTGTTC TCCACCCTTG
GGACCCCCTG GAGGAAATGG TCATTTTTGT GGCAGTCATG CTTCATCAAC TCTGTTCACC
TCGGATAATC AAATGTTTGT TCAGTTTATT TCTGATCACA GTAATGAAGG GCAAGGATTT
AAAATCAAAT ATGAGGCAAA GAGTTTAGCC TGTGGGGGCA ACGTCTACAT CCATGATGCT
GATTCTGCTG GGTATGTGAC CTCCCCCAAC CACCCTCATA ATTATCCCCC GCACGCTGAT
TGCATTTGGA TCTTAGCGGC TCCACCGGAA ACACGCATAC AGCTGCAATT TGAAGATCGA
TTCGATATTG AAGTAACACC CAACTGTACT TCCAACTACC TTGAGTTGCG GGATGGAGTG
GATTCGGATG CACCAATACT TTCCAAATTT TGTGGGACAT CTTTGCCCAG CAGTCAGTGG
TCCTCAGGAG AGGTTATGTA TTTGAGATTT CGATCTGACA ACAGCCCCAC ACATGTGGGA
TTCAAGGCCA AGTATTCTAT AGCTCAGTGT GGGGGAAGAG TACCAGGGCA AAGTGGTGTT
GTTGAAAGCA TTGGACATCC AACACTTCCA TACAGAGACA ACTTATTCTG TGAGTGGCAT
CTCCAGGGGC TCTCTGGACA CTATCTCACC ATCTCTTTTG AAGACTTTAA CCTTCAGAAT
TCTTCTGGCT GTGAAAAAGA CTTCGTGGAG ATCTGGGACA ATCATACCTC TGGAAACATC
TTGGGCAGAT ACTGTGGAAA CACCATTCCT GACAGCATAG ACACTTCTAG CAATACTGCT
GTGGTCAGGT TTGTCACAGA CGGCTCTGTG ACTGCCTCAG GATTCAGACT GCGATTTGAA
TCCAGTATGG AAGAGTGTGG TGGGGATCTT CAGGGCTCTA TTGGAACATT TACTTCTCCC
AACTACCCGA ACCCAAATCC TCATGGCCGG ATCTGCGAGT GGAGAATCAC TGCCCCGGAG
GGAAGGCGGA TCACCCTAAT GTTTAACAAC CTGAGGCTGG CCACGCATCC GTCCTGCAAC
AATGAGCATG TGATAGTATT CAATGGCATT AGAAGTAACT CACCCCAGCT AGAGAAACTG
TGTAGTAGTG TGAATGTAAG CAATGAGATT AAATCTTCAG GAAACACAAT GAAAGTCATT
TTTTTCACGG ATGGATCCAG GCCATATGGC GGCTTCACTG CTTCCTATAC CTCCAGTGAA
GATGCAGTGT GTGGTGGGTC TCTTCCAAAT ACTCCTGAAG GAAACTTTAC TTCTCCTGGC
TATGACGGAG TCAGGAATTA CTCAAGAAAC CTGAACTGCG AATGGACTCT CAGCAATCCA
AATCAGGGAA ATTCATCCAT TTCCATTCAC TTTGAAGATT TTTACCTAGA AAGTCACCAA
GACTGTCAAT TTGATGTCCT CGAGTTTCGA GTGGGTGATG CTGATGGGCC CCTGATGTGG
AGACTTTGTG GTCCTTCAAA GCCTACATTG CCATTGGTTA TACCTTATTC TCAGGTATGG
ATTCACTTTG TCACCAACGA ACGTGTAGAA CACATTGGAT TCCATGCAAA GTATTCCTTT
ACAGATTGTG GCGGAATACA GATAGGTGAC AGTGGAGTGA TCACAAGCCC CAACTATCCA
AATGCTTATG ACAGCCTGAC CCACTGCTCT TCGCTGTTGG AGGCCCCACA AGGGCACACC
ATCACTCTCA CATTTAGTGA CTTTGATATT GAACCCCATA CAACTTGTGC TTGGGACTCT
GTCACTGTCA GGAATGGTGG GTCCCCTGAA TCACCCATCA TAGGACAATA CTGTGGAAAT
TCAAACCCCA GGACAATACA GTCAGGTTCC AATCAGCTGG TCGTGACTTT TAACTCAGAC
CATTCATTGC AAGGTGGTGG ATTTTATGCT ACGTGGAACA CACAAACTTT AGGTTGTGGT
GGAATATTTC ATTCTGATAA TGGTACAATC AGATCCCCTC ACTGGCCTCA GAATTTTCCC
GAAAACAGCA GATGTTCCTG GACGGCCATT ACTCACAAAA GTAAACACTT GGAGATCAGC
TTTGACAACA ACTTCCTAAT CCCCAGCGGT GATGGACAAT GTCAGAATAG CTTCGTGAAG
GTGTGGGCAG GAACTGAGGA GGTGGACAAA GCCCTGCTAG CCACTGGCTG TGGGAACGTG
GCTCCGGGTC CCGTTATCAC ACCAAGTAAC ACATTCACTG CCGTCTTCCA GTCTCAGGAG
GCACCAGCTC AGGGCTTCTC CGCGTCCTTT GTTAGCCGAT GTGGAAGTAA TTTCACTGGC
CCTTCAGGTT ACATCATTTC TCCAAATTAC CCAAAACAAT ATGACAACAA CATGAATTGC
ACCTATGTCA TAGAGGCTAA TCCTCTGTCA GTGGTCCTCT TGACTTTTGT GTCCTTCCAC
TTAGAAGCTC GTTCCGCTGT GACGGGAAGC TGTGTCAACG ATGGCGTGCA CATTATCAGA
GGTTACAGCG TCATGTCCAC CCCATTTGCT ACTGTGTGTG GGGATGAGAT GCCAGCTCCC
CTCACCATCG CTGGGCCGGT TCTGCTTAAC TTCTACTCCA ACGAGCAAAT CACAGACTTC
GGATTCAAGT TTTCCTATAG GATAATCTCC TGTGGTGGTG TGTTCAATTT CTCTTCTGGA
ATCATCACAA GTCCTGCCTA TTCATACGCA GACTACCCAA ATGATATGCA CTGTCTGTAT
ACCATCACCG TTAGTGACGA CAAGGTGATC GAGCTCAAGT TCAGTGATTT TGATGTGGTT
CCCTCCACCT CCTGCTCCCA TGACTACCTG GCAATTTACG ATGGTGCCAA TACCAGCGAT
CCCCTTCTTG GCAAATTCTG CGGTTCCAAG CGCCCACCAA ATGTGAAGAG CAGCAATAAT
AGTATGCTCC TGGTGTTCAA GACAGATTCA TTTCAGACAG CAAAAGGCTG GAAGATGTCT
TTCCGGCAGA CATTGGGGCC TCAGCAAGGA TGTGGTGGTT ATCTGACAGG CTCGAATAAT
ACCTTTGCCT CTCCTGATTC TGATTCGAAT GGAATGTATG ACAAGAATTT AAACTGTGTA
TGGATCATAA TTGCACCTGT AAACAAAGTA ATTCACCTCA CCTTCAATAC ATTTGCTCTG
GAGGCAGCAA GTACTAGGCA AAGATGCCTT TATGATTATG TAAAGTTATA TGATGGGGAT
AGTGAAAATG CGAACTTGGC TGGAACGTTT TGTGGTTCCA CAGTACCTGC TCCTTTTATC
TCTTCTGGTA ACTTCCTTAC GGTTCAATTC ATCAGTGACT TAACATTAGA GAGGGAAGGA
TTTAATGCTA CATACACCAT CATGGACATG CCTTGTGGTG GAACATACAA TGCAACTTGG
ACCCCACAAA ATATTTCATC ACCCAATTCA TCAGACCCAG ATGTCCCATT TTCCATCTGT
ACTTGGGTCA TTGATTCCCC TCCGCATCAG CAGGTCAAGA TAACTGTGTG GGCATTACAG
CTGACCTCGC AAGACTGCAC GCAGAATTAC TTACAGCTTC AGGACTCACC GCAGGGTCAC
GGAAATTCAA GATTTCAGTT CTGTGGCAGA AATGCTTCGG CTGTGCCAGT GTTTTATTCT
TCTATGAGTA CTGCAATGGT CATTTTCAAA TCTGGAGTTG TAAACAGAAA CTCTAGAATG
AGTTTCACCT ATCAGATTGC AGATTGCAAC AGAGACTATC ACAAGGCATT TGGCAACCTG
AGAAGCCCTG GATGGCCAGA TAACTACGAC AATGACAAGG ATTGCACCGT TACTCTCACA
GCCCCCCAGA ACCACACCAT TTCCCTCTTT TTTCATTCAC TTGGCATCGA GAACTCAGTT
GAATGCAGAA ACGATTTCTT GGAGGTGAGA AATGGAAGTA ACAGCAATTC ACCATTACTG
GGCAAGTACT GTGGAACTCT GCTGCCAAAC CCTGTCTTCT CTCAAAATAA TGAACTATAC
CTACGATTTA AGAGTGATAG TGTAACTTCT GATCGTGGAT ATGAAATCAT CTGGACTTCA
TCACCCTCTG GATGTGGTGG AACTCTTTAT GGAGACAGAG GCTCATTCAC CAGCCCCGGC
TATCCAGGCA CATACCCAAA CAACACGTAC TGCGAGTGGG TCCTTGTTGC TCCTGCTGGA
AGGCTTGTCA CCATCAACTT CTACTTCATC AGCATTGACG ATCCAGGAGA CTGTGTCCAG
AACTATCTCA CACTCTATGA TGGGCCCAAC GCCAGCTCTC CATCCTCTGG ACCATACTGC
GGAGGCGACA CCAGCATAGC TCCCTTCGTG GCTTCCTCAA ATCAGGTCTT CATAAAATTT
CATGCTGATT ATGCACGGCG TCCATCCGCA TTCCGATTAA CTTGGGACAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518013.1
CDS872..7585
Misc Feature(1)884..1228(+)
Misc Feature(2)914..1228(+)
Misc Feature(3)1241..1564(+)
Misc Feature(4)1265..1564(+)
Misc Feature(5)1571..1912(+)
Misc Feature(6)1598..1912(+)
Misc Feature(7)1925..2254(+)
Misc Feature(8)1949..2251(+)
Misc Feature(9)2288..2599(+)
Misc Feature(10)2291..2599(+)
Misc Feature(11)2645..2977(+)
Misc Feature(12)2675..2974(+)
Misc Feature(13)2987..3349(+)
Misc Feature(14)3017..3349(+)
Misc Feature(15)3362..3712(+)
Misc Feature(16)3398..3712(+)
Misc Feature(17)3719..4054(+)
Misc Feature(18)3743..4054(+)
Misc Feature(19)4067..4405(+)
Misc Feature(20)4097..4405(+)
Misc Feature(21)4421..4771(+)
Misc Feature(22)4448..4771(+)
Misc Feature(23)4778..5113(+)
Misc Feature(24)4802..5113(+)
Misc Feature(25)5126..5467(+)
Misc Feature(26)5150..5467(+)
Misc Feature(27)5471..5815(+)
Misc Feature(28)5495..5815(+)
Misc Feature(29)5822..6157(+)
Misc Feature(30)5846..6157(+)
Misc Feature(31)6182..6532(+)
Misc Feature(32)6209..6532(+)
Misc Feature(33)6545..6889(+)
Misc Feature(34)6575..6889(+)
Misc Feature(35)6896..7231(+)
Misc Feature(36)6920..7231(+)
Misc Feature(37)7244..7576(+)
Misc Feature(38)7274..7573(+)
Translation

Target ORF information:

RefSeq Version XM_011519711
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cubilin (intrinsic factor-cobalamin receptor) (CUBN), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011519711

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
ATGATCCAAG GTTGTGGTGG AGAGCTGTCT GGGGCCACAG GCTCCTTCAG CAGCCCCGGG 
TTCCCCAACA GGTATCCACC AAACAAGGAG TGTATCTGGT ACATTAGGAC GGACCCCGGG
AGTAGCATTC AGCTCACCAT CCATGACTTC GATGTGGAGT ATCATTCAAG GTGCAACTTT
GATGTCTTGG AGATCTATGG AGGCCCCGAT TTCCACTCTC CCAGAATAGC CCAACTGTGT
ACCCAGAGAT CACCTGAGAA CCCCATGCAG GTCTCCAGCA CTGGAAATGA GCTAGCAATT
CGATTCAAGA CCGACTTGTC CATAAATGGG AGAGGCTTCA ATGCGTCATG GCAAGCAGTC
ACTGGAGGTT GTGGTGGGAT TTTCCAGGCT CCCAGTGGAG AGATTCATTC TCCAAATTAC
CCCAGTCCTT ATAGGAGCAA CACAGACTGT TCTTGGGTCA TTCGGGTTGA CAGAAATCAT
CGTGTTCTCT TGAACTTCAC TGACTTTGAT CTTGAACCAC AAGACTCTTG TATTATGGCA
TACGATGGCT TAAGCTCCAC AATGTCCCGC CTTGCCAGGA CGTGTGGAAG GGAGCAGCTG
GCTAACCCCA TCGTCTCCTC AGGAAACAGC CTCTTCTTGA GATTTCAGTC TGGCCCTTCC
AGACAGAACA GAGGCTTCCG AGCTCAATTC AGGCAAGCCT GCGGAGGCCA CATCCTCACC
AGCTCATTTG ATACTGTTTC CTCTCCACGG TTCCCTGCCA ATTATCCAAA CAATCAGAAC
TGCAGCTGGA TCATTCAAGC GCAACCTCCA TTAAATCATA TCACCCTCTC TTTTACCCAC
TTTGAACTTG AAAGAAGCAC AACGTGTGCA CGTGACTTTG TAGAAATTTT GGATGGCGGC
CACGAAGACG CGCCCCTCCG AGGCCGTTAC TGTGGCACCG ACATGCCCCA TCCTATCACA
TCCTTCAGCA GCGCCCTGAC GCTGAGATTC GTCTCTGATT CTAGCATCAG TGCTGGGGGT
TTCCACACCA CGGTCACCGC ATCAGTGTCG GCTTGTGGTG GAACGTTCTA CATGGCTGAA
GGCATCTTCA ACAGCCCTGG CTACCCAGAC ATTTATCCCC CTAATGTGGA ATGTGTCTGG
AACATCGTCA GTTCCCCTGG CAACCGGCTC CAGCTGTCTT TTATATCTTT CCAGTTGGAA
GACTCTCAGG ACTGCAGCAG AGATTTTGTG GAGATCCGTG AAGGAAATGC CACGGGTCAC
TTGGTGGGAC GATACTGTGG AAACTCCTTC CCTCTCAATT ATTCTTCCAT CGTTGGACAT
ACCCTGTGGG TCAGATTTAT CTCAGATGGT TCTGGCAGCG GCACGGGCTT CCAGGCCACA
TTTATGAAGA TATTTGGCAA TGATAATATT GTGGGAACTC ATGGGAAAGT CGCCTCTCCT
TTCTGGCCTG AAAACTACCC ACATAACTCC AATTACCAAT GGACAGTAAA TGTGAATGCA
TCTCACGTTG TCCATGGTAG AATCTTGGAG ATGGACATAG AAGAAATACA AAACTGCTAT
TATGACAAAT TAAGGATCTA TGATGGGCCT AGCATTCACG CCCGCCTAAT TGGAGCTTAC
TGTGGTACCC AGACTGAATC TTTCAGCTCC ACTGGAAATT CTTTGACATT TCATTTTTAC
TCCGACTCTT CAATCTCAGG GAAGGGATTC CTTCTGGAGT GGTTTGCAGT GGATGCACCT
GATGGTGTTT TACCTACCAT TGCTCCAGGT GCTTGTGGTG GCTTCCTGAG GACGGGAGAT
GCACCCGTGT TTCTCTTCTC CCCGGGCTGG CCTGACAGTT ACAGTAATAG AGTGGACTGT
ACGTGGCTCA TCCAGGCTCC CGACTCTACC GTGGAACTCA ACATTCTTTC CCTGGACATT
GAATCTCACC GAACGTGTGC CTATGATAGC CTTGTGATAC GAGATGGAGA TAATAACTTG
GCCCAGCAGC TAGCAGTTCT CTGTGGCAGA GAGATCCCTG GGCCCATCCG GTCTACTGGA
GAGTACATGT TCATCCGCTT CACCTCGGAC TCCAGTGTAA CCAGGGCAGG CTTCAATGCA
TCCTTTCACA AGAGCTGCGG TGGATATTTG CATGCAGACA GAGGGATCAT CACGTCCCCC
AAGTATCCAG AGACTTACCC ATCCAACCTC AACTGTTCTT GGCACGTCCT GGTCCAAAGT
GGCCTGACCA TTGCTGTCCA TTTTGAACAG CCTTTCCAGA TTCCAAATGG AGATTCTTCT
TGCAACCAGG GGGATTACTT GGTGCTAAGA AATGGTCCTG ATATCTGTTC TCCACCCTTG
GGACCCCCTG GAGGAAATGG TCATTTTTGT GGCAGTCATG CTTCATCAAC TCTGTTCACC
TCGGATAATC AAATGTTTGT TCAGTTTATT TCTGATCACA GTAATGAAGG GCAAGGATTT
AAAATCAAAT ATGAGGCAAA GAGTTTAGCC TGTGGGGGCA ACGTCTACAT CCATGATGCT
GATTCTGCTG GGTATGTGAC CTCCCCCAAC CACCCTCATA ATTATCCCCC GCACGCTGAT
TGCATTTGGA TCTTAGCGGC TCCACCGGAA ACACGCATAC AGCTGCAATT TGAAGATCGA
TTCGATATTG AAGTAACACC CAACTGTACT TCCAACTACC TTGAGTTGCG GGATGGAGTG
GATTCGGATG CACCAATACT TTCCAAATTT TGTGGGACAT CTTTGCCCAG CAGTCAGTGG
TCCTCAGGAG AGGTTATGTA TTTGAGATTT CGATCTGACA ACAGCCCCAC ACATGTGGGA
TTCAAGGCCA AGTATTCTAT AGCTCAGTGT GGGGGAAGAG TACCAGGGCA AAGTGGTGTT
GTTGAAAGCA TTGGACATCC AACACTTCCA TACAGAGACA ACTTATTCTG TGAGTGGCAT
CTCCAGGGGC TCTCTGGACA CTATCTCACC ATCTCTTTTG AAGACTTTAA CCTTCAGAAT
TCTTCTGGCT GTGAAAAAGA CTTCGTGGAG ATCTGGGACA ATCATACCTC TGGAAACATC
TTGGGCAGAT ACTGTGGAAA CACCATTCCT GACAGCATAG ACACTTCTAG CAATACTGCT
GTGGTCAGGT TTGTCACAGA CGGCTCTGTG ACTGCCTCAG GATTCAGACT GCGATTTGAA
TCCAGTATGG AAGAGTGTGG TGGGGATCTT CAGGGCTCTA TTGGAACATT TACTTCTCCC
AACTACCCGA ACCCAAATCC TCATGGCCGG ATCTGCGAGT GGAGAATCAC TGCCCCGGAG
GGAAGGCGGA TCACCCTAAT GTTTAACAAC CTGAGGCTGG CCACGCATCC GTCCTGCAAC
AATGAGCATG TGATAGTATT CAATGGCATT AGAAGTAACT CACCCCAGCT AGAGAAACTG
TGTAGTAGTG TGAATGTAAG CAATGAGATT AAATCTTCAG GAAACACAAT GAAAGTCATT
TTTTTCACGG ATGGATCCAG GCCATATGGC GGCTTCACTG CTTCCTATAC CTCCAGTGAA
GATGCAGTGT GTGGTGGGTC TCTTCCAAAT ACTCCTGAAG GAAACTTTAC TTCTCCTGGC
TATGACGGAG TCAGGAATTA CTCAAGAAAC CTGAACTGCG AATGGACTCT CAGCAATCCA
AATCAGGGAA ATTCATCCAT TTCCATTCAC TTTGAAGATT TTTACCTAGA AAGTCACCAA
GACTGTCAAT TTGATGTCCT CGAGTTTCGA GTGGGTGATG CTGATGGGCC CCTGATGTGG
AGACTTTGTG GTCCTTCAAA GCCTACATTG CCATTGGTTA TACCTTATTC TCAGGTATGG
ATTCACTTTG TCACCAACGA ACGTGTAGAA CACATTGGAT TCCATGCAAA GTATTCCTTT
ACAGATTGTG GCGGAATACA GATAGGTGAC AGTGGAGTGA TCACAAGCCC CAACTATCCA
AATGCTTATG ACAGCCTGAC CCACTGCTCT TCGCTGTTGG AGGCCCCACA AGGGCACACC
ATCACTCTCA CATTTAGTGA CTTTGATATT GAACCCCATA CAACTTGTGC TTGGGACTCT
GTCACTGTCA GGAATGGTGG GTCCCCTGAA TCACCCATCA TAGGACAATA CTGTGGAAAT
TCAAACCCCA GGACAATACA GTCAGGTTCC AATCAGCTGG TCGTGACTTT TAACTCAGAC
CATTCATTGC AAGGTGGTGG ATTTTATGCT ACGTGGAACA CACAAACTTT AGGTTGTGGT
GGAATATTTC ATTCTGATAA TGGTACAATC AGATCCCCTC ACTGGCCTCA GAATTTTCCC
GAAAACAGCA GATGTTCCTG GACGGCCATT ACTCACAAAA GTAAACACTT GGAGATCAGC
TTTGACAACA ACTTCCTAAT CCCCAGCGGT GATGGACAAT GTCAGAATAG CTTCGTGAAG
GTGTGGGCAG GAACTGAGGA GGTGGACAAA GCCCTGCTAG CCACTGGCTG TGGGAACGTG
GCTCCGGGTC CCGTTATCAC ACCAAGTAAC ACATTCACTG CCGTCTTCCA GTCTCAGGAG
GCACCAGCTC AGGGCTTCTC CGCGTCCTTT GTTAGCCGAT GTGGAAGTAA TTTCACTGGC
CCTTCAGGTT ACATCATTTC TCCAAATTAC CCAAAACAAT ATGACAACAA CATGAATTGC
ACCTATGTCA TAGAGGCTAA TCCTCTGTCA GTGGTCCTCT TGACTTTTGT GTCCTTCCAC
TTAGAAGCTC GTTCCGCTGT GACGGGAAGC TGTGTCAACG ATGGCGTGCA CATTATCAGA
GGTTACAGCG TCATGTCCAC CCCATTTGCT ACTGTGTGTG GGGATGAGAT GCCAGCTCCC
CTCACCATCG CTGGGCCGGT TCTGCTTAAC TTCTACTCCA ACGAGCAAAT CACAGACTTC
GGATTCAAGT TTTCCTATAG GATAATCTCC TGTGGTGGTG TGTTCAATTT CTCTTCTGGA
ATCATCACAA GTCCTGCCTA TTCATACGCA GACTACCCAA ATGATATGCA CTGTCTGTAT
ACCATCACCG TTAGTGACGA CAAGGTGATC GAGCTCAAGT TCAGTGATTT TGATGTGGTT
CCCTCCACCT CCTGCTCCCA TGACTACCTG GCAATTTACG ATGGTGCCAA TACCAGCGAT
CCCCTTCTTG GCAAATTCTG CGGTTCCAAG CGCCCACCAA ATGTGAAGAG CAGCAATAAT
AGTATGCTCC TGGTGTTCAA GACAGATTCA TTTCAGACAG CAAAAGGCTG GAAGATGTCT
TTCCGGCAGA CATTGGGGCC TCAGCAAGGA TGTGGTGGTT ATCTGACAGG CTCGAATAAT
ACCTTTGCCT CTCCTGATTC TGATTCGAAT GGAATGTATG ACAAGAATTT AAACTGTGTA
TGGATCATAA TTGCACCTGT AAACAAAGTA ATTCACCTCA CCTTCAATAC ATTTGCTCTG
GAGGCAGCAA GTACTAGGCA AAGATGCCTT TATGATTATG TAAAGTTATA TGATGGGGAT
AGTGAAAATG CGAACTTGGC TGGAACGTTT TGTGGTTCCA CAGTACCTGC TCCTTTTATC
TCTTCTGGTA ACTTCCTTAC GGTTCAATTC ATCAGTGACT TAACATTAGA GAGGGAAGGA
TTTAATGCTA CATACACCAT CATGGACATG CCTTGTGGTG GAACATACAA TGCAACTTGG
ACCCCACAAA ATATTTCATC ACCCAATTCA TCAGACCCAG ATGTCCCATT TTCCATCTGT
ACTTGGGTCA TTGATTCCCC TCCGCATCAG CAGGTCAAGA TAACTGTGTG GGCATTACAG
CTGACCTCGC AAGACTGCAC GCAGAATTAC TTACAGCTTC AGGACTCACC GCAGGGTCAC
GGAAATTCAA GATTTCAGTT CTGTGGCAGA AATGCTTCGG CTGTGCCAGT GTTTTATTCT
TCTATGAGTA CTGCAATGGT CATTTTCAAA TCTGGAGTTG TAAACAGAAA CTCTAGAATG
AGTTTCACCT ATCAGATTGC AGATTGCAAC AGAGACTATC ACAAGGCATT TGGCAACCTG
AGAAGCCCTG GATGGCCAGA TAACTACGAC AATGACAAGG ATTGCACCGT TACTCTCACA
GCCCCCCAGA ACCACACCAT TTCCCTCTTT TTTCATTCAC TTGGCATCGA GAACTCAGTT
GAATGCAGAA ACGATTTCTT GGAGGTGAGA AATGGAAGTA ACAGCAATTC ACCATTACTG
GGCAAGTACT GTGGAACTCT GCTGCCAAAC CCTGTCTTCT CTCAAAATAA TGAACTATAC
CTACGATTTA AGAGTGATAG TGTAACTTCT GATCGTGGAT ATGAAATCAT CTGGACTTCA
TCACCCTCTG GATGTGGTGG AACTCTTTAT GGAGACAGAG GCTCATTCAC CAGCCCCGGC
TATCCAGGCA CATACCCAAA CAACACGTAC TGCGAGTGGG TCCTTGTTGC TCCTGCTGGA
AGGCTTGTCA CCATCAACTT CTACTTCATC AGCATTGACG ATCCAGGAGA CTGTGTCCAG
AACTATCTCA CACTCTATGA TGGGCCCAAC GCCAGCTCTC CATCCTCTGG ACCATACTGC
GGAGGCGACA CCAGCATAGC TCCCTTCGTG GCTTCCTCAA ATCAGGTCTT CATAAAATTT
CATGCTGATT ATGCACGGCG TCCATCCGCA TTCCGATTAA CTTGGGACAG CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Genetic Associations with Plasma B12, B6, and Folate Levels in an Ischemic Stroke Population from the Vitamin Intervention for Stroke Prevention (VISP) Trial
Front Public Health 2, 112 (2014)
Keene KL, Chen WM, Chen F, Williams SR, Elkhatib SD, Hsu FC, Mychaleckyj JC, Doheny KF, Pugh EW, Ling H, Laurie CC, Gogarten SM, Madden EB, Worrall BB and Sale MM.


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Hum. Genet. 133 (7), 883-893 (2014)
Grant AV, Cobat A, Van Thuc N, Orlova M, Huong NT, Gaschignard J, Alter A, Ba NN, Thai VH, Abel L, Alcais A and Schurr E.


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Cubilin maintains blood levels of HDL and albumin
J. Am. Soc. Nephrol. 25 (5), 1028-1036 (2014)
Aseem O, Smith BT, Cooley MA, Wilkerson BA, Argraves KM, Remaley AT and Argraves WS.


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PLoS ONE 9 (2), E88332 (2014)
Wang J, Zhao JY, Wang F, Peng QQ, Hou J, Sun SN, Gui YH, Duan WY, Qiao B and Wang HY.


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Discovery and refinement of loci associated with lipid levels
Nat. Genet. 45 (11), 1274-1283 (2013)
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The intrinsic factor-vitamin B12 receptor and target of teratogenic antibodies is a megalin-binding peripheral membrane protein with homology to developmental proteins
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Characterization of an epithelial approximately 460-kDa protein that facilitates endocytosis of intrinsic factor-vitamin B12 and binds receptor-associated protein
J. Biol. Chem. 272 (42), 26497-26504 (1997)
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The CUB domain. A widespread module in developmentally regulated proteins
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