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PDHX pyruvate dehydrogenase complex, component X [Homo sapiens (human)]

Gene Symbol PDHX
Entrez Gene ID 8050
Full Name pyruvate dehydrogenase complex, component X
Synonyms DLDBP, E3BP, OPDX, PDX1, proX
General protein information
Preferred Names
pyruvate dehydrogenase protein X component, mitochondrial
Names
pyruvate dehydrogenase protein X component, mitochondrial
pyruvate dehydrogenase complex, E3-binding protein subunit
lipoyl-containing pyruvate dehydrogenase complex component X
pyruvate dehydrogenase complex, lipoyl-containing component X
dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p13

Summary The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP). This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]. lac of sum
Disorder MIM:

608769

Disorder Html: Lacticacidemia due to PDX1 deficiency, 245349 (3)





The following PDHX gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDHX gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu61608 XM_011520390 PREDICTED: Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu19013 NM_003477 Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu07230 NM_001135024 Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $409
OHu10429 NM_001166158 Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $309

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu61608
Accession Version XM_011520390.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1326bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase protein X component, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA 
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT
GCCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011518692.1
CDS131..1456
Misc Feature(1)131..1450(+)
Misc Feature(2)131..343(+)
Misc Feature(3)209..259(+)
Misc Feature(4)239..241(+)
Misc Feature(5)491..604(+)
Misc Feature(6)821..1450(+)
Translation
Position Chain Variation Link
18 18 c, t dbSNP:745671139
33 33 a, g dbSNP:143842617
38 38 c, t dbSNP:561326145
41 41 a, g dbSNP:3763931
50 50 c, g dbSNP:550296114
65 65 c, g dbSNP:569927061
68 68 c, t dbSNP:542669183
105 105 c, t dbSNP:551228102
111 111 a, g dbSNP:771011394
128 128 c, g dbSNP:775669398
130 130 a, g dbSNP:747128117
136 136 a, g dbSNP:768751336
141 141 g, t dbSNP:776981891
144 144 c, g dbSNP:762057004
152 152 a, g dbSNP:765643071
153 153 c, t dbSNP:773591910
164 164 a, c dbSNP:143301712
175 175 a, g dbSNP:574905086
187 187 a, g dbSNP:750973777
192 192 a, g dbSNP:554051809
195 195 a, t dbSNP:778685760
198 198 c, t dbSNP:745866603
199 199 a, g dbSNP:376628638
200 200 a, g dbSNP:200562821
204 204 g, t dbSNP:748233840
215 215 a, g dbSNP:139199177
217 217 a, g dbSNP:111829480
220 220 a, t dbSNP:778024968
232 232 a, g dbSNP:749644848
236 236 a, g dbSNP:771320500
245 245 c, g, t dbSNP:373211545
251 251 a, g dbSNP:11539202
252 252 c, t dbSNP:61752924
258 258 a, g dbSNP:566746801
267 267 a, g dbSNP:146456454
269 269 a, g dbSNP:72912954
271 271 a, t dbSNP:201359586
275 275 a, t dbSNP:748447623
279 279 c, t dbSNP:753585930
286 286 -, aatc dbSNP:752635475
289 289 c, t dbSNP:34582941
290 290 a, g dbSNP:765036658
293 293 a, g dbSNP:762712381
304 304 a, t dbSNP:766332522
308 308 a, g dbSNP:751534737
315 315 a, c, t dbSNP:756159990
316 316 a, g dbSNP:567206438
317 317 c, t dbSNP:773313977
318 318 a, g dbSNP:150462412
324 324 g, t dbSNP:746240660
328 328 a, g dbSNP:758731703
339 339 g, t dbSNP:780685781
340 340 a, g dbSNP:747470313
343 343 a, g dbSNP:769322264
345 345 c, t dbSNP:776217794
352 352 -, agg dbSNP:746633600
352 352 a, g dbSNP:768560478
353 353 c, g dbSNP:769476738
361 361 c, t dbSNP:145394719
366 366 a, g dbSNP:762775345
369 369 -, at dbSNP:756985515
369 369 a, t dbSNP:199531388
370 370 c, t dbSNP:766133140
372 372 a, t dbSNP:774151027
380 380 a, c dbSNP:759429254
384 384 a, c, g dbSNP:371030372
388 388 c, t dbSNP:75182779
396 396 c, t dbSNP:191524984
423 423 a, t dbSNP:750662542
424 424 a, g, t dbSNP:148723565
428 428 c, t dbSNP:769945697
429 429 a, g dbSNP:747386411
435 435 c, t dbSNP:755488601
448 448 a, g dbSNP:781780010
451 451 a, t dbSNP:139085745
453 453 -, ccatc dbSNP:780555750
456 456 c, t dbSNP:769225702
457 457 c, t dbSNP:79170416
458 458 a, c dbSNP:748839506
461 461 a, g dbSNP:142256229
470 470 a, g dbSNP:774065524
472 472 a, g dbSNP:540300624
476 476 a, g dbSNP:767427196
479 479 c, t dbSNP:775331560
481 481 c, t dbSNP:76486106
482 482 a, g dbSNP:765182974
485 485 a, g dbSNP:374629778
486 486 c, t dbSNP:763022273
487 487 a, g dbSNP:766725538
491 491 c, t dbSNP:548929441
492 492 a, g dbSNP:148117169
496 496 c, t dbSNP:375932576
498 498 a, c, g dbSNP:141719797
512 512 a, g dbSNP:778357262
515 515 c, t dbSNP:150572021
516 516 a, g, t dbSNP:61752925
519 519 a, g dbSNP:745475982
527 527 c, g dbSNP:149337104
529 529 a, g dbSNP:61752926
533 533 c, t dbSNP:746837316
539 539 a, c, g dbSNP:139052284
540 540 c, t dbSNP:763097165
543 543 a, g dbSNP:771180919
546 546 c, g dbSNP:187115311
557 557 a, c dbSNP:200008900
567 567 c, g dbSNP:767781507
568 568 c, g dbSNP:143997835
570 570 -, c dbSNP:724159979
572 572 c, t dbSNP:61752927
573 573 a, g dbSNP:367620723
590 590 c, g dbSNP:146445744
594 594 a, g, t dbSNP:200568558
603 603 a, g dbSNP:762240579
608 608 c, g dbSNP:765747426
614 614 c, t dbSNP:749926043
620 620 c, g dbSNP:757998291
624 624 c, t dbSNP:148645836
625 625 a, g, t dbSNP:558284287
626 626 a, g dbSNP:112573215
627 627 g, t dbSNP:781092468
629 629 a, c dbSNP:201706378
638 638 a, g dbSNP:370210752
649 649 a, g dbSNP:777915154
651 651 c, t dbSNP:142914598
652 652 a, c, t dbSNP:772231144
653 653 c, g dbSNP:747215797
654 654 c, t dbSNP:768765711
656 656 -, c dbSNP:776567343
656 656 c, g dbSNP:113722325
657 657 a, c dbSNP:151100443
659 659 a, c dbSNP:527753201
661 661 a, c, g dbSNP:200018992
662 662 a, c dbSNP:118054792
664 664 a, g dbSNP:762351343
681 681 c, t dbSNP:765867082
684 684 c, t dbSNP:141096005
687 687 a, c dbSNP:759159888
690 690 c, t dbSNP:767288617
692 692 c, g, t dbSNP:113309941
694 694 a, g dbSNP:756038747
699 699 c, t dbSNP:146876119
701 701 g, t dbSNP:1049311
702 702 -, ctggaccat dbSNP:765244722
702 702 c, t dbSNP:372896186
703 703 c, t dbSNP:757174659
706 706 a, g dbSNP:780114827
708 708 c, g dbSNP:777713368
716 716 c, t dbSNP:147958676
719 719 c, t dbSNP:768910663
720 720 a, g dbSNP:141506224
730 730 c, t dbSNP:748483350
738 738 c, g, t dbSNP:770197938
739 739 a, g dbSNP:755428861
741 741 c, t dbSNP:763473883
743 743 a, g dbSNP:377147669
744 744 c, g dbSNP:183059121
748 748 c, t dbSNP:61760973
750 750 -, tagtgt dbSNP:758373003
750 750 g, t dbSNP:767272326
759 759 a, g dbSNP:138205941
764 764 g, t dbSNP:752227276
787 787 c, t dbSNP:371579798
788 788 a, g dbSNP:375993336
795 795 a, g dbSNP:149176285
798 798 c, t dbSNP:769015094
800 800 c, t dbSNP:143131258
801 801 a, g dbSNP:763853325
804 804 a, g dbSNP:148234977
806 806 c, g dbSNP:776474910
808 808 c, t dbSNP:497582
817 817 a, g dbSNP:765193763
822 822 c, t dbSNP:750379822
824 824 a, c dbSNP:758358727
829 829 a, g dbSNP:767547817
831 831 c, g dbSNP:111270862
839 839 a, g dbSNP:146262415
840 840 c, g, t dbSNP:777912667
842 842 a, g dbSNP:757601482
844 844 a, g dbSNP:779450982
860 860 a, g dbSNP:746327927
869 869 g, t dbSNP:772736821
877 877 a, t dbSNP:564799362
879 879 a, g dbSNP:533564681
907 907 a, g dbSNP:36047324
910 910 c, g dbSNP:776188525
912 912 a, g dbSNP:761649691
920 920 a, g dbSNP:768103465
922 922 c, t dbSNP:780778970
926 926 c, g dbSNP:35560997
929 929 c, t dbSNP:769495018
934 934 a, g dbSNP:772929171
940 940 c, t dbSNP:570773289
940 940 -, t dbSNP:748631357
944 944 -, atc dbSNP:772751309
954 954 c, t dbSNP:150518427
957 957 c, t dbSNP:770848200
963 963 a, t dbSNP:372862365
982 982 a, g dbSNP:771477240
984 984 a, t dbSNP:556425857
997 997 c, t dbSNP:765526287
998 998 c, t dbSNP:139509954
1002 1002 a, g, t dbSNP:200866298
1005 1005 c, g dbSNP:377367930
1007 1007 c, g dbSNP:149705022
1009 1009 c, g dbSNP:755386042
1011 1011 a, g dbSNP:200189510
1018 1018 a, g dbSNP:781749544
1024 1024 a, g dbSNP:752140130
1025 1025 c, t dbSNP:755687648
1027 1027 a, t dbSNP:777380555
1043 1043 a, g dbSNP:180948326
1046 1046 a, g dbSNP:756932261
1050 1050 g, t dbSNP:201532817
1053 1053 c, g dbSNP:745688585
1059 1059 a, t dbSNP:17850649
1061 1061 a, g dbSNP:771899300
1064 1064 a, g dbSNP:201070810
1066 1066 -, ctta dbSNP:771202510
1075 1075 -, t dbSNP:776854797
1077 1077 c, t dbSNP:775537821
1078 1078 a, t dbSNP:748156460
1079 1079 a, g dbSNP:75430333
1088 1088 c, g dbSNP:202188706
1098 1098 c, g dbSNP:773459344
1108 1108 c, t dbSNP:763203759
1114 1114 a, g dbSNP:766725349
1138 1138 a, g dbSNP:200434738
1145 1145 a, g dbSNP:761101485
1147 1147 a, g dbSNP:764597215
1149 1149 c, t dbSNP:753333049
1154 1154 c, g dbSNP:756801221
1166 1166 g, t dbSNP:141752861
1175 1175 c, g dbSNP:750044524
1181 1181 c, t dbSNP:758020436
1183 1183 a, g dbSNP:779835379
1187 1187 a, g dbSNP:751327601
1189 1189 a, t dbSNP:148758785
1195 1195 g, t dbSNP:781350102
1206 1206 a, g dbSNP:745949756
1208 1208 a, t dbSNP:772326730
1209 1209 c, t dbSNP:780208101
1213 1213 a, g, t dbSNP:373894917
1216 1216 a, g dbSNP:533512853
1221 1221 g, t dbSNP:762238484
1223 1223 a, g dbSNP:368548573
1225 1225 c, t dbSNP:772761912
1226 1226 a, g dbSNP:762433166
1228 1228 c, t dbSNP:766019303
1229 1229 a, g dbSNP:371811647
1232 1232 c, t dbSNP:553438817
1236 1236 c, g dbSNP:375438296
1238 1238 a, g dbSNP:369989142
1242 1242 c, t dbSNP:756023441
1250 1250 c, g dbSNP:372175732
1272 1272 c, t dbSNP:750513249
1273 1273 a, g dbSNP:200276180
1274 1274 g, t dbSNP:374793216
1281 1281 a, g dbSNP:34398018
1283 1283 g, t dbSNP:147948716
1287 1287 a, g dbSNP:368909312
1301 1301 a, c, g dbSNP:748567027
1307 1307 c, g dbSNP:773627664
1314 1314 a, t dbSNP:748813806
1318 1318 a, g dbSNP:544330039
1332 1332 c, g, t dbSNP:141886009
1339 1339 a, g dbSNP:767292826
1340 1340 c, t dbSNP:775413853
1341 1341 a, g dbSNP:760454961
1349 1349 a, g dbSNP:764061627
1353 1353 c, t dbSNP:201928307
1359 1359 a, c dbSNP:757248125
1368 1368 c, g dbSNP:755241681
1376 1376 c, t dbSNP:766377898
1379 1379 c, g dbSNP:751649853
1389 1389 a, g dbSNP:755176882
1390 1390 c, t dbSNP:369787288
1400 1400 a, g dbSNP:748511132
1405 1405 a, g dbSNP:756480324
1407 1407 g, t dbSNP:778226421
1412 1412 a, g dbSNP:145345995
1433 1433 a, g dbSNP:771573054
1442 1442 a, g dbSNP:773943684
1445 1445 c, t dbSNP:374272120
1446 1446 a, g dbSNP:377638805
1448 1448 c, t dbSNP:775323800
1453 1453 c, t dbSNP:151049569
1456 1456 g, t dbSNP:763755572
1463 1463 -, aagat dbSNP:764087598
1463 1463 a, g dbSNP:546073588
1470 1470 g, t dbSNP:776489344
1483 1483 a, c, g dbSNP:74524673
1484 1484 a, g dbSNP:751631448
1490 1490 c, t dbSNP:376037131
1492 1492 c, g dbSNP:767700777
1500 1500 a, g dbSNP:752818327
1502 1502 g, t dbSNP:756393903
1503 1503 c, t dbSNP:777935430
1542 1542 -, t dbSNP:139648571
1570 1570 a, g dbSNP:76663700
1579 1579 a, g dbSNP:59918794
1629 1629 a, g dbSNP:75863418
1656 1656 c, t dbSNP:16926653
1688 1688 c, t dbSNP:550736007
1715 1715 g, t dbSNP:771515806
1787 1787 c, g dbSNP:555691861
1849 1849 a, g dbSNP:539067497
1942 1942 g, t dbSNP:9326
1943 1943 a, g dbSNP:567069522
1956 1956 c, t dbSNP:536022201
1964 1964 -, aata dbSNP:149231678
1971 1971 a, g dbSNP:369448429
1984 1984 a, c dbSNP:1063351
2000 2000 c, t dbSNP:575464356
2028 2028 a, g dbSNP:544287125
2051 2051 -, t dbSNP:562174425
2100 2100 a, c dbSNP:7808
2159 2159 a, g dbSNP:558824877
2165 2165 a, g dbSNP:762642480
2181 2181 a, g dbSNP:763837848
2191 2191 a, g dbSNP:3210067
2198 2198 c, t dbSNP:577566893
2199 2199 a, g dbSNP:577162977
2202 2202 c, t dbSNP:370785794
2218 2218 c, t dbSNP:3210068
2218 2218 c, t dbSNP:559562945
2239 2239 a, g dbSNP:751318367
2281 2281 a, g dbSNP:531264127
2295 2295 c, t dbSNP:956130
2301 2301 a, g dbSNP:757212170
2314 2314 c, t dbSNP:189194127
2320 2320 a, g dbSNP:542135463
2321 2321 a, t dbSNP:778661951
2346 2346 c, t dbSNP:1049333
2360 2360 g, t dbSNP:182283782

Target ORF information:

RefSeq Version XM_011520390
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520390

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA 
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT
GCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19013
Accession Version NM_003477.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1506bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase protein X component, mitochondrial isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107928.3, BC010389.1, AF001437.1 and AA282215.1. This sequence is a reference standard in the RefSeqGene project. On Sep 17, 2008 this sequence version replaced gi:4505698. Summary: The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP). This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BX648910.1, U82328.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT
GCCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_003468.2
CDS527..2032
Misc Feature(1)344..346(+)
Misc Feature(2)695..919(+)
Misc Feature(3)698..2026(+)
Misc Feature(4)785..835(+)
Misc Feature(5)815..817(+)
Misc Feature(6)1067..1180(+)
Misc Feature(7)1073..1168(+)
Misc Feature(8)1106..1108(+)
Misc Feature(9)1397..2026(+)
Exon (1)1..686
Gene:PDHX
Gene Synonym:
Exon (2)687..767
Gene:PDHX
Gene Synonym:
Exon (3)768..868
Gene:PDHX
Gene Synonym:
Exon (4)869..1068
Gene:PDHX
Gene Synonym:
Exon (5)1069..1167
Gene:PDHX
Gene Synonym:
Exon (6)1168..1342
Gene:PDHX
Gene Synonym:
Exon (7)1343..1490
Gene:PDHX
Gene Synonym:
Exon (8)1491..1549
Gene:PDHX
Gene Synonym:
Exon (9)1550..1708
Gene:PDHX
Gene Synonym:
Exon (10)1709..1773
Gene:PDHX
Gene Synonym:
Exon (11)1774..2988
Gene:PDHX
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, c, g, t dbSNP:35924042
8 8 a, c dbSNP:561463212
21 21 ca, tg dbSNP:386752391
21 21 c, t dbSNP:2016814
22 22 a, g dbSNP:16926448
27 27 c, t dbSNP:563821039
37 37 c, t dbSNP:374157413
47 47 c, t dbSNP:375386462
48 48 a, g dbSNP:780056321
55 55 c, t dbSNP:552157217
61 61 c, t dbSNP:769982440
69 69 a, g dbSNP:773273824
75 75 c, g dbSNP:749560956
76 76 c, g dbSNP:771164728
81 81 c, t dbSNP:774655472
82 82 a, g dbSNP:759895240
94 94 c, t dbSNP:199893242
97 97 a, g dbSNP:202241286
99 99 c, t dbSNP:760074931
102 102 c, g dbSNP:763711108
105 105 a, g, t dbSNP:146420175
114 114 c, t dbSNP:756933277
116 116 a, g dbSNP:139123979
120 120 a, t dbSNP:369975644
124 124 c, t dbSNP:758274248
130 130 c, t dbSNP:779852629
134 134 -, c dbSNP:764336742
134 134 c, t dbSNP:747005576
137 137 a, g dbSNP:2956114
146 146 a, t dbSNP:777780994
148 148 a, c dbSNP:749401268
151 151 a, g dbSNP:771070943
156 156 g, t dbSNP:774565620
162 162 a, g dbSNP:746202661
164 164 c, t dbSNP:772530666
168 168 a, g dbSNP:775840738
173 173 c, t dbSNP:761199870
178 178 c, g dbSNP:764543470
185 185 a, g dbSNP:567329509
187 187 c, t dbSNP:761422179
190 190 c, g dbSNP:764971293
192 192 g, t dbSNP:750168506
196 196 a, g dbSNP:762622673
197 197 c, g dbSNP:766242750
198 198 a, c dbSNP:751333457
202 202 a, c, g dbSNP:753186558
206 206 a, c, g dbSNP:753853669
207 207 a, c, t dbSNP:779100145
208 208 a, g, t dbSNP:2956113
209 209 a, g dbSNP:747343062
212 212 a, c, t dbSNP:769228399
215 215 a, g dbSNP:761405893
219 219 c, t dbSNP:555955830
220 220 c, g dbSNP:772976341
221 221 a, g dbSNP:762615901
223 223 a, t dbSNP:765979127
225 225 a, g dbSNP:2956112
226 226 c, g dbSNP:759259296
227 227 a, g, t dbSNP:767425029
245 245 a, g dbSNP:757293776
250 250 c, g dbSNP:538595954
252 252 g, t dbSNP:750504158
255 255 a, g dbSNP:2956111
256 256 a, g, t dbSNP:780153979
270 270 c, t dbSNP:769211875
271 271 -, g dbSNP:752007834
278 278 a, g dbSNP:572118408
288 288 a, g dbSNP:756796889
319 319 c, t dbSNP:555380381
325 325 a, g dbSNP:113073242
327 327 a, g dbSNP:772737420
335 335 c, g dbSNP:574902434
340 340 c, g dbSNP:544017835
357 357 g, t dbSNP:770555008
362 362 a, g dbSNP:773929855
365 365 a, c dbSNP:759318998
370 370 c, t dbSNP:563586329
383 383 -, ggct dbSNP:762116656
386 386 c, t dbSNP:775289563
387 387 -, gggttgg dbSNP:767922429
390 390 g, t dbSNP:760569642
392 392 -, gt dbSNP:750683422
395 395 -, gggc dbSNP:756201233
395 395 a, g dbSNP:764026434
396 396 -, ggc dbSNP:139999735
396 396 -, gt dbSNP:754980282
397 397 -, gc dbSNP:753674694
398 398 -, cgg dbSNP:371074122
398 398 c, g, t dbSNP:112032820
399 399 -, ggggg dbSNP:778548879
403 403 c, g dbSNP:375130125
404 404 g, t dbSNP:758466385
428 428 a, t dbSNP:559322053
439 439 a, c dbSNP:2956110
460 460 c, g, t dbSNP:547276719
477 477 a, g dbSNP:766620539
480 480 a, c dbSNP:751771341
481 481 c, g, t dbSNP:764604412
484 484 a, c dbSNP:781647178
486 486 a, c, g dbSNP:748553521
487 487 c, g, t dbSNP:369228797
492 492 a, c, g dbSNP:372814513
494 494 a, g dbSNP:774021266
500 500 -, g dbSNP:747994483
500 500 c, t dbSNP:529801753
501 501 a, g, t dbSNP:377596373
503 503 c, g dbSNP:760481755
504 504 a, c dbSNP:201033364
505 505 a, c dbSNP:776499519
506 506 a, g dbSNP:763059743
511 511 g, t dbSNP:569788862
519 519 c, t dbSNP:766421119
520 520 c, t dbSNP:751680964
523 523 c, g dbSNP:3818401
528 528 c, t dbSNP:558713973
531 531 c, g dbSNP:753053135
532 532 a, g dbSNP:756600157
535 535 c, t dbSNP:778335059
539 539 g, t dbSNP:749796976
545 545 c, g dbSNP:147403982
548 548 g, t dbSNP:778248643
549 549 c, g dbSNP:374254725
555 555 a, c dbSNP:139613834
561 561 c, g dbSNP:200419151
564 564 c, t dbSNP:775185079
570 570 a, g dbSNP:387906998
573 573 a, c dbSNP:118136428
575 575 c, t dbSNP:182569882
581 581 g, t dbSNP:761609180
586 586 a, c dbSNP:769800412
588 588 c, t dbSNP:774504089
589 589 c, t dbSNP:2956109
593 593 c, t dbSNP:1049306
594 594 a, g dbSNP:753030741
596 596 c, g dbSNP:11539201
597 597 a, g dbSNP:764412516
608 608 c, t dbSNP:754249799
613 613 -, gaag dbSNP:724159828
615 615 a, g dbSNP:757640533
616 616 a, g, t dbSNP:374923275
619 619 g, t dbSNP:377527030
623 623 c, t dbSNP:757970464
626 626 c, g dbSNP:779650616
629 629 a, t dbSNP:746715011
634 634 c, t dbSNP:1049307
635 635 g, t dbSNP:780807263
642 642 c, g dbSNP:747977950
643 643 c, t dbSNP:769559458
644 644 a, g dbSNP:773340684
655 655 g, t dbSNP:759679470
681 681 a, g, t dbSNP:772315120
686 686 a, g dbSNP:146678221
687 687 a, g dbSNP:771011394
704 704 c, g dbSNP:775669398
706 706 a, g dbSNP:747128117
712 712 a, g dbSNP:768751336
717 717 g, t dbSNP:776981891
720 720 c, g dbSNP:762057004
728 728 a, g dbSNP:765643071
729 729 c, t dbSNP:773591910
740 740 a, c dbSNP:143301712
751 751 a, g dbSNP:574905086
763 763 a, g dbSNP:750973777
768 768 a, g dbSNP:554051809
771 771 a, t dbSNP:778685760
774 774 c, t dbSNP:745866603
775 775 a, g dbSNP:376628638
776 776 a, g dbSNP:200562821
780 780 g, t dbSNP:748233840
791 791 a, g dbSNP:139199177
793 793 a, g dbSNP:111829480
796 796 a, t dbSNP:778024968
808 808 a, g dbSNP:749644848
812 812 a, g dbSNP:771320500
821 821 c, g, t dbSNP:373211545
827 827 a, g dbSNP:11539202
828 828 c, t dbSNP:61752924
834 834 a, g dbSNP:566746801
843 843 a, g dbSNP:146456454
845 845 a, g dbSNP:72912954
847 847 a, t dbSNP:201359586
851 851 a, t dbSNP:748447623
855 855 c, t dbSNP:753585930
862 862 -, aatc dbSNP:752635475
865 865 c, t dbSNP:34582941
866 866 a, g dbSNP:765036658
869 869 a, g dbSNP:762712381
880 880 a, t dbSNP:766332522
884 884 a, g dbSNP:751534737
891 891 a, c, t dbSNP:756159990
892 892 a, g dbSNP:567206438
893 893 c, t dbSNP:773313977
894 894 a, g dbSNP:150462412
900 900 g, t dbSNP:746240660
904 904 a, g dbSNP:758731703
915 915 g, t dbSNP:780685781
916 916 a, g dbSNP:747470313
919 919 a, g dbSNP:769322264
921 921 c, t dbSNP:776217794
928 928 -, agg dbSNP:746633600
928 928 a, g dbSNP:768560478
929 929 c, g dbSNP:769476738
937 937 c, t dbSNP:145394719
942 942 a, g dbSNP:762775345
945 945 -, at dbSNP:756985515
945 945 a, t dbSNP:199531388
946 946 c, t dbSNP:766133140
948 948 a, t dbSNP:774151027
956 956 a, c dbSNP:759429254
960 960 a, c, g dbSNP:371030372
964 964 c, t dbSNP:75182779
972 972 c, t dbSNP:191524984
999 999 a, t dbSNP:750662542
1000 1000 a, g, t dbSNP:148723565
1004 1004 c, t dbSNP:769945697
1005 1005 a, g dbSNP:747386411
1011 1011 c, t dbSNP:755488601
1024 1024 a, g dbSNP:781780010
1027 1027 a, t dbSNP:139085745
1029 1029 -, ccatc dbSNP:780555750
1032 1032 c, t dbSNP:769225702
1033 1033 c, t dbSNP:79170416
1034 1034 a, c dbSNP:748839506
1037 1037 a, g dbSNP:142256229
1046 1046 a, g dbSNP:774065524
1048 1048 a, g dbSNP:540300624
1052 1052 a, g dbSNP:767427196
1055 1055 c, t dbSNP:775331560
1057 1057 c, t dbSNP:76486106
1058 1058 a, g dbSNP:765182974
1061 1061 a, g dbSNP:374629778
1062 1062 c, t dbSNP:763022273
1063 1063 a, g dbSNP:766725538
1067 1067 c, t dbSNP:548929441
1068 1068 a, g dbSNP:148117169
1072 1072 c, t dbSNP:375932576
1074 1074 a, c, g dbSNP:141719797
1088 1088 a, g dbSNP:778357262
1091 1091 c, t dbSNP:150572021
1092 1092 a, g, t dbSNP:61752925
1095 1095 a, g dbSNP:745475982
1103 1103 c, g dbSNP:149337104
1105 1105 a, g dbSNP:61752926
1109 1109 c, t dbSNP:746837316
1115 1115 a, c, g dbSNP:139052284
1116 1116 c, t dbSNP:763097165
1119 1119 a, g dbSNP:771180919
1122 1122 c, g dbSNP:187115311
1133 1133 a, c dbSNP:200008900
1143 1143 c, g dbSNP:767781507
1144 1144 c, g dbSNP:143997835
1146 1146 -, c dbSNP:724159979
1148 1148 c, t dbSNP:61752927
1149 1149 a, g dbSNP:367620723
1166 1166 c, g dbSNP:146445744
1170 1170 a, g, t dbSNP:200568558
1179 1179 a, g dbSNP:762240579
1184 1184 c, g dbSNP:765747426
1190 1190 c, t dbSNP:749926043
1196 1196 c, g dbSNP:757998291
1200 1200 c, t dbSNP:148645836
1201 1201 a, g, t dbSNP:558284287
1202 1202 a, g dbSNP:112573215
1203 1203 g, t dbSNP:781092468
1205 1205 a, c dbSNP:201706378
1214 1214 a, g dbSNP:370210752
1225 1225 a, g dbSNP:777915154
1227 1227 c, t dbSNP:142914598
1228 1228 a, c, t dbSNP:772231144
1229 1229 c, g dbSNP:747215797
1230 1230 c, t dbSNP:768765711
1232 1232 -, c dbSNP:776567343
1232 1232 c, g dbSNP:113722325
1233 1233 a, c dbSNP:151100443
1235 1235 a, c dbSNP:527753201
1237 1237 a, c, g dbSNP:200018992
1238 1238 a, c dbSNP:118054792
1240 1240 a, g dbSNP:762351343
1257 1257 c, t dbSNP:765867082
1260 1260 c, t dbSNP:141096005
1263 1263 a, c dbSNP:759159888
1266 1266 c, t dbSNP:767288617
1268 1268 c, g, t dbSNP:113309941
1270 1270 a, g dbSNP:756038747
1275 1275 c, t dbSNP:146876119
1277 1277 g, t dbSNP:1049311
1278 1278 -, ctggaccat dbSNP:765244722
1278 1278 c, t dbSNP:372896186
1279 1279 c, t dbSNP:757174659
1282 1282 a, g dbSNP:780114827
1284 1284 c, g dbSNP:777713368
1292 1292 c, t dbSNP:147958676
1295 1295 c, t dbSNP:768910663
1296 1296 a, g dbSNP:141506224
1306 1306 c, t dbSNP:748483350
1314 1314 c, g, t dbSNP:770197938
1315 1315 a, g dbSNP:755428861
1317 1317 c, t dbSNP:763473883
1319 1319 a, g dbSNP:377147669
1320 1320 c, g dbSNP:183059121
1324 1324 c, t dbSNP:61760973
1326 1326 -, tagtgt dbSNP:758373003
1326 1326 g, t dbSNP:767272326
1335 1335 a, g dbSNP:138205941
1340 1340 g, t dbSNP:752227276
1363 1363 c, t dbSNP:371579798
1364 1364 a, g dbSNP:375993336
1371 1371 a, g dbSNP:149176285
1374 1374 c, t dbSNP:769015094
1376 1376 c, t dbSNP:143131258
1377 1377 a, g dbSNP:763853325
1380 1380 a, g dbSNP:148234977
1382 1382 c, g dbSNP:776474910
1384 1384 c, t dbSNP:497582
1393 1393 a, g dbSNP:765193763
1398 1398 c, t dbSNP:750379822
1400 1400 a, c dbSNP:758358727
1405 1405 a, g dbSNP:767547817
1407 1407 c, g dbSNP:111270862
1415 1415 a, g dbSNP:146262415
1416 1416 c, g, t dbSNP:777912667
1418 1418 a, g dbSNP:757601482
1420 1420 a, g dbSNP:779450982
1436 1436 a, g dbSNP:746327927
1445 1445 g, t dbSNP:772736821
1453 1453 a, t dbSNP:564799362
1455 1455 a, g dbSNP:533564681
1483 1483 a, g dbSNP:36047324
1486 1486 c, g dbSNP:776188525
1488 1488 a, g dbSNP:761649691
1496 1496 a, g dbSNP:768103465
1498 1498 c, t dbSNP:780778970
1502 1502 c, g dbSNP:35560997
1505 1505 c, t dbSNP:769495018
1510 1510 a, g dbSNP:772929171
1516 1516 c, t dbSNP:570773289
1516 1516 -, t dbSNP:748631357
1520 1520 -, atc dbSNP:772751309
1530 1530 c, t dbSNP:150518427
1533 1533 c, t dbSNP:770848200
1539 1539 a, t dbSNP:372862365
1558 1558 a, g dbSNP:771477240
1560 1560 a, t dbSNP:556425857
1573 1573 c, t dbSNP:765526287
1574 1574 c, t dbSNP:139509954
1578 1578 a, g, t dbSNP:200866298
1581 1581 c, g dbSNP:377367930
1583 1583 c, g dbSNP:149705022
1585 1585 c, g dbSNP:755386042
1587 1587 a, g dbSNP:200189510
1594 1594 a, g dbSNP:781749544
1600 1600 a, g dbSNP:752140130
1601 1601 c, t dbSNP:755687648
1603 1603 a, t dbSNP:777380555
1619 1619 a, g dbSNP:180948326
1622 1622 a, g dbSNP:756932261
1626 1626 g, t dbSNP:201532817
1629 1629 c, g dbSNP:745688585
1635 1635 a, t dbSNP:17850649
1637 1637 a, g dbSNP:771899300
1640 1640 a, g dbSNP:201070810
1642 1642 -, ctta dbSNP:771202510
1651 1651 -, t dbSNP:776854797
1653 1653 c, t dbSNP:775537821
1654 1654 a, t dbSNP:748156460
1655 1655 a, g dbSNP:75430333
1664 1664 c, g dbSNP:202188706
1674 1674 c, g dbSNP:773459344
1684 1684 c, t dbSNP:763203759
1690 1690 a, g dbSNP:766725349
1714 1714 a, g dbSNP:200434738
1721 1721 a, g dbSNP:761101485
1723 1723 a, g dbSNP:764597215
1725 1725 c, t dbSNP:753333049
1730 1730 c, g dbSNP:756801221
1742 1742 g, t dbSNP:141752861
1751 1751 c, g dbSNP:750044524
1757 1757 c, t dbSNP:758020436
1759 1759 a, g dbSNP:779835379
1763 1763 a, g dbSNP:751327601
1765 1765 a, t dbSNP:148758785
1771 1771 g, t dbSNP:781350102
1782 1782 a, g dbSNP:745949756
1784 1784 a, t dbSNP:772326730
1785 1785 c, t dbSNP:780208101
1789 1789 a, g, t dbSNP:373894917
1792 1792 a, g dbSNP:533512853
1797 1797 g, t dbSNP:762238484
1799 1799 a, g dbSNP:368548573
1801 1801 c, t dbSNP:772761912
1802 1802 a, g dbSNP:762433166
1804 1804 c, t dbSNP:766019303
1805 1805 a, g dbSNP:371811647
1808 1808 c, t dbSNP:553438817
1812 1812 c, g dbSNP:375438296
1814 1814 a, g dbSNP:369989142
1818 1818 c, t dbSNP:756023441
1826 1826 c, g dbSNP:372175732
1848 1848 c, t dbSNP:750513249
1849 1849 a, g dbSNP:200276180
1850 1850 g, t dbSNP:374793216
1857 1857 a, g dbSNP:34398018
1859 1859 g, t dbSNP:147948716
1863 1863 a, g dbSNP:368909312
1877 1877 a, c, g dbSNP:748567027
1883 1883 c, g dbSNP:773627664
1890 1890 a, t dbSNP:748813806
1894 1894 a, g dbSNP:544330039
1908 1908 c, g, t dbSNP:141886009
1915 1915 a, g dbSNP:767292826
1916 1916 c, t dbSNP:775413853
1917 1917 a, g dbSNP:760454961
1925 1925 a, g dbSNP:764061627
1929 1929 c, t dbSNP:201928307
1935 1935 a, c dbSNP:757248125
1944 1944 c, g dbSNP:755241681
1952 1952 c, t dbSNP:766377898
1955 1955 c, g dbSNP:751649853
1965 1965 a, g dbSNP:755176882
1966 1966 c, t dbSNP:369787288
1976 1976 a, g dbSNP:748511132
1981 1981 a, g dbSNP:756480324
1983 1983 g, t dbSNP:778226421
1988 1988 a, g dbSNP:145345995
2009 2009 a, g dbSNP:771573054
2018 2018 a, g dbSNP:773943684
2021 2021 c, t dbSNP:374272120
2022 2022 a, g dbSNP:377638805
2024 2024 c, t dbSNP:775323800
2029 2029 c, t dbSNP:151049569
2032 2032 g, t dbSNP:763755572
2039 2039 -, aagat dbSNP:764087598
2039 2039 a, g dbSNP:546073588
2046 2046 g, t dbSNP:776489344
2059 2059 a, c, g dbSNP:74524673
2060 2060 a, g dbSNP:751631448
2066 2066 c, t dbSNP:376037131
2068 2068 c, g dbSNP:767700777
2076 2076 a, g dbSNP:752818327
2078 2078 g, t dbSNP:756393903
2079 2079 c, t dbSNP:777935430
2118 2118 -, t dbSNP:139648571
2146 2146 a, g dbSNP:76663700
2155 2155 a, g dbSNP:59918794
2205 2205 a, g dbSNP:75863418
2232 2232 c, t dbSNP:16926653
2264 2264 c, t dbSNP:550736007
2291 2291 g, t dbSNP:771515806
2363 2363 c, g dbSNP:555691861
2425 2425 a, g dbSNP:539067497
2518 2518 g, t dbSNP:9326
2519 2519 a, g dbSNP:567069522
2532 2532 c, t dbSNP:536022201
2540 2540 -, aata dbSNP:149231678
2547 2547 a, g dbSNP:369448429
2560 2560 a, c dbSNP:1063351
2576 2576 c, t dbSNP:575464356
2604 2604 a, g dbSNP:544287125
2627 2627 -, t dbSNP:562174425
2676 2676 a, c dbSNP:7808
2735 2735 a, g dbSNP:558824877
2741 2741 a, g dbSNP:762642480
2757 2757 a, g dbSNP:763837848
2767 2767 a, g dbSNP:3210067
2774 2774 c, t dbSNP:577566893
2775 2775 a, g dbSNP:577162977
2778 2778 c, t dbSNP:370785794
2794 2794 c, t dbSNP:3210068
2794 2794 c, t dbSNP:559562945
2815 2815 a, g dbSNP:751318367
2857 2857 a, g dbSNP:531264127
2871 2871 c, t dbSNP:956130
2877 2877 a, g dbSNP:757212170
2890 2890 c, t dbSNP:189194127
2896 2896 a, g dbSNP:542135463
2897 2897 a, t dbSNP:778661951
2922 2922 c, t dbSNP:1049333
2936 2936 g, t dbSNP:182283782

Target ORF information:

RefSeq Version NM_003477
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003477

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT
GCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu07230
Accession Version NM_001135024.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase protein X component, mitochondrial isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA573433.1, BI915296.1, U79296.1 and AA282215.1. Summary: The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP). This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]. Transcript Variant: This variant (2) lacks a segment in the 5' region, resulting in upstream in-frame AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAATCAG GCGGCGCTGA GGGCAGCCCG GGGGCGGGGC GAACGGGGCG GGGGCCGGGG 
TCTGGTAAGG CCCCGCCGGC TGAGATATCC AGCGGCGCAC CTGACTTCCC GGGAGGTGAT
CCCATTAAGA TACTAATGCC ATCACTGTCT CCTACAATGG AAGAAGGAAA CATTGTGAAA
TGGCTGAAAA AGGAAGGTGA AGCGGTGAGT GCTGGAGATG CATTATGTGA AATTGAGACT
GACAAAGCTG TGGTTACCTT AGATGCAAGT GATGATGGAA TCTTGGCCAA AATCGTGGTT
GAAGAAGGAA GTAAAAATAT ACGGCTAGGT TCACTAATTG GTTTGATAGT AGAAGAAGGA
GAAGATTGGA AACATGTTGA AATTCCCAAA GACGTAGGTC CTCCACCACC AGTTTCAAAA
CCTTCAGAGC CTCGCCCCTC ACCAGAACCA CAGATTTCCA TCCCTGTCAA GAAGGAACAC
ATACCCGGGA CACTACGGTT CCGTTTAAGT CCAGCTGCCC GCAATATTCT GGAAAAACAC
TCACTGGATG CTAGCCAGGG CACAGCCACT GGCCCTCGGG GGATATTCAC TAAAGAGGAT
GCTCTCAAAC TTGTCCAGTT GAAACAAACG GGCAAGATTA CCGAGTCCAG ACCAACTCCA
GCCCCCACAG CCACTCCCAC AGCACCTTCG CCCCTACAGG CCACAGCTGG ACCATCTTAT
CCCCGGCCTG TGATCCCACC AGTATCAACT CCTGGACAAC CCAATGCAGT GGGCACATTC
ACTGAAATCC CCGCCAGCAA TATTCGAAGA GTTATTGCCA AGAGATTAAC TGAATCTAAA
AGTACTGTAC CTCATGCATA TGCTACTGCT GACTGTGACC TTGGAGCTGT TTTAAAAGTT
AGGCAAGATC TGGTCAAAGA TGACATTAAA GTATCAGTAA ATGATTTTAT CATCAAGGCA
GCAGCTGTTA CCCTTAAACA AATGCCAGAT GTTAATGTAA GCTGGGATGG AGAGGGCCCA
AAGCAACTGC CATTTATTGA CATTTCAGTG GCTGTGGCAA CAGATAAAGG CTTACTTACT
CCAATCATAA AAGATGCTGC TGCTAAAGGT ATCCAGGAAA TTGCTGACTC TGTAAAGGCT
CTATCAAAGA AAGCAAGAGA TGGAAAATTG TTGCCTGAAG AATACCAAGG AGGATCTTTT
AGTATTTCCA ACTTGGGGAT GTTTGGCATC GACGAATTTA CTGCAGTGAT TAACCCTCCT
CAGGCCTGCA TTTTGGCGGT TGGGAGGTTC CGACCTGTGC TGAAGCTCAC TGAGGATGAA
GAGGGAAATG CCAAACTGCA GCAGCGCCAG CTCATAACAG TCACAATGTC AAGTGACAGT
CGAGTGGTTG ATGACGAACT GGCAACCAGG TTTCTTAAAA GTTTTAAAGC AAACCTAGAG
AATCCTATCC GACTTGCCTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001128496.1
CDS243..1703
Misc Feature(1)366..590(+)
Misc Feature(2)369..1697(+)
Misc Feature(3)456..506(+)
Misc Feature(4)486..488(+)
Misc Feature(5)738..851(+)
Misc Feature(6)1068..1697(+)
Exon (1)1..357
Gene:PDHX
Gene Synonym:
Exon (2)358..438
Gene:PDHX
Gene Synonym:
Exon (3)439..539
Gene:PDHX
Gene Synonym:
Exon (4)540..739
Gene:PDHX
Gene Synonym:
Exon (5)740..838
Gene:PDHX
Gene Synonym:
Exon (6)839..1013
Gene:PDHX
Gene Synonym:
Exon (7)1014..1161
Gene:PDHX
Gene Synonym:
Exon (8)1162..1220
Gene:PDHX
Gene Synonym:
Exon (9)1221..1379
Gene:PDHX
Gene Synonym:
Exon (10)1380..1444
Gene:PDHX
Gene Synonym:
Exon (11)1445..2659
Gene:PDHX
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, c, g, t dbSNP:35924042
8 8 a, c dbSNP:561463212
21 21 ca, tg dbSNP:386752391
21 21 c, t dbSNP:2016814
22 22 a, g dbSNP:16926448
27 27 c, t dbSNP:563821039
37 37 c, t dbSNP:374157413
47 47 c, t dbSNP:375386462
48 48 a, g dbSNP:780056321
55 55 c, t dbSNP:552157217
61 61 c, t dbSNP:769982440
69 69 a, g dbSNP:773273824
75 75 c, g dbSNP:749560956
76 76 c, g dbSNP:771164728
81 81 c, t dbSNP:774655472
82 82 a, g dbSNP:759895240
94 94 c, t dbSNP:199893242
97 97 a, g dbSNP:202241286
99 99 c, t dbSNP:760074931
102 102 c, g dbSNP:763711108
105 105 a, g, t dbSNP:146420175
114 114 c, t dbSNP:756933277
116 116 a, g dbSNP:139123979
120 120 a, t dbSNP:369975644
124 124 c, t dbSNP:758274248
130 130 c, t dbSNP:779852629
134 134 -, c dbSNP:764336742
134 134 c, t dbSNP:747005576
137 137 a, g dbSNP:2956114
146 146 a, t dbSNP:777780994
148 148 a, c dbSNP:749401268
151 151 a, g dbSNP:771070943
156 156 g, t dbSNP:774565620
162 162 a, g dbSNP:746202661
164 164 c, t dbSNP:772530666
168 168 a, g dbSNP:775840738
173 173 c, t dbSNP:761199870
178 178 c, g dbSNP:764543470
185 185 a, g dbSNP:567329509
187 187 c, t dbSNP:761422179
190 190 c, g dbSNP:764971293
192 192 g, t dbSNP:750168506
196 196 a, g dbSNP:762622673
197 197 c, g dbSNP:766242750
198 198 a, c dbSNP:751333457
202 202 a, c, g dbSNP:753186558
206 206 a, c, g dbSNP:753853669
207 207 a, c, t dbSNP:779100145
208 208 a, g, t dbSNP:2956113
209 209 a, g dbSNP:747343062
212 212 a, c, t dbSNP:769228399
215 215 a, g dbSNP:761405893
219 219 c, t dbSNP:555955830
220 220 c, g dbSNP:772976341
221 221 a, g dbSNP:762615901
223 223 a, t dbSNP:765979127
225 225 a, g dbSNP:2956112
226 226 c, g dbSNP:759259296
227 227 a, g, t dbSNP:767425029
245 245 a, g dbSNP:757293776
250 250 c, g dbSNP:538595954
252 252 g, t dbSNP:750504158
255 255 a, g dbSNP:2956111
256 256 a, g, t dbSNP:780153979
270 270 c, t dbSNP:769211875
271 271 -, g dbSNP:752007834
278 278 a, g dbSNP:572118408
288 288 a, g dbSNP:756796889
319 319 c, t dbSNP:555380381
325 325 a, g dbSNP:113073242
327 327 a, g dbSNP:772737420
335 335 c, g dbSNP:574902434
340 340 c, g dbSNP:544017835
357 357 g, t dbSNP:770555008
358 358 a, g dbSNP:771011394
375 375 c, g dbSNP:775669398
377 377 a, g dbSNP:747128117
383 383 a, g dbSNP:768751336
388 388 g, t dbSNP:776981891
391 391 c, g dbSNP:762057004
399 399 a, g dbSNP:765643071
400 400 c, t dbSNP:773591910
411 411 a, c dbSNP:143301712
422 422 a, g dbSNP:574905086
434 434 a, g dbSNP:750973777
439 439 a, g dbSNP:554051809
442 442 a, t dbSNP:778685760
445 445 c, t dbSNP:745866603
446 446 a, g dbSNP:376628638
447 447 a, g dbSNP:200562821
451 451 g, t dbSNP:748233840
462 462 a, g dbSNP:139199177
464 464 a, g dbSNP:111829480
467 467 a, t dbSNP:778024968
479 479 a, g dbSNP:749644848
483 483 a, g dbSNP:771320500
492 492 c, g, t dbSNP:373211545
498 498 a, g dbSNP:11539202
499 499 c, t dbSNP:61752924
505 505 a, g dbSNP:566746801
514 514 a, g dbSNP:146456454
516 516 a, g dbSNP:72912954
518 518 a, t dbSNP:201359586
522 522 a, t dbSNP:748447623
526 526 c, t dbSNP:753585930
533 533 -, aatc dbSNP:752635475
536 536 c, t dbSNP:34582941
537 537 a, g dbSNP:765036658
540 540 a, g dbSNP:762712381
551 551 a, t dbSNP:766332522
555 555 a, g dbSNP:751534737
562 562 a, c, t dbSNP:756159990
563 563 a, g dbSNP:567206438
564 564 c, t dbSNP:773313977
565 565 a, g dbSNP:150462412
571 571 g, t dbSNP:746240660
575 575 a, g dbSNP:758731703
586 586 g, t dbSNP:780685781
587 587 a, g dbSNP:747470313
590 590 a, g dbSNP:769322264
592 592 c, t dbSNP:776217794
599 599 -, agg dbSNP:746633600
599 599 a, g dbSNP:768560478
600 600 c, g dbSNP:769476738
608 608 c, t dbSNP:145394719
613 613 a, g dbSNP:762775345
616 616 -, at dbSNP:756985515
616 616 a, t dbSNP:199531388
617 617 c, t dbSNP:766133140
619 619 a, t dbSNP:774151027
627 627 a, c dbSNP:759429254
631 631 a, c, g dbSNP:371030372
635 635 c, t dbSNP:75182779
643 643 c, t dbSNP:191524984
670 670 a, t dbSNP:750662542
671 671 a, g, t dbSNP:148723565
675 675 c, t dbSNP:769945697
676 676 a, g dbSNP:747386411
682 682 c, t dbSNP:755488601
695 695 a, g dbSNP:781780010
698 698 a, t dbSNP:139085745
700 700 -, ccatc dbSNP:780555750
703 703 c, t dbSNP:769225702
704 704 c, t dbSNP:79170416
705 705 a, c dbSNP:748839506
708 708 a, g dbSNP:142256229
717 717 a, g dbSNP:774065524
719 719 a, g dbSNP:540300624
723 723 a, g dbSNP:767427196
726 726 c, t dbSNP:775331560
728 728 c, t dbSNP:76486106
729 729 a, g dbSNP:765182974
732 732 a, g dbSNP:374629778
733 733 c, t dbSNP:763022273
734 734 a, g dbSNP:766725538
738 738 c, t dbSNP:548929441
739 739 a, g dbSNP:148117169
743 743 c, t dbSNP:375932576
745 745 a, c, g dbSNP:141719797
759 759 a, g dbSNP:778357262
762 762 c, t dbSNP:150572021
763 763 a, g, t dbSNP:61752925
766 766 a, g dbSNP:745475982
774 774 c, g dbSNP:149337104
776 776 a, g dbSNP:61752926
780 780 c, t dbSNP:746837316
786 786 a, c, g dbSNP:139052284
787 787 c, t dbSNP:763097165
790 790 a, g dbSNP:771180919
793 793 c, g dbSNP:187115311
804 804 a, c dbSNP:200008900
814 814 c, g dbSNP:767781507
815 815 c, g dbSNP:143997835
817 817 -, c dbSNP:724159979
819 819 c, t dbSNP:61752927
820 820 a, g dbSNP:367620723
837 837 c, g dbSNP:146445744
841 841 a, g, t dbSNP:200568558
850 850 a, g dbSNP:762240579
855 855 c, g dbSNP:765747426
861 861 c, t dbSNP:749926043
867 867 c, g dbSNP:757998291
871 871 c, t dbSNP:148645836
872 872 a, g, t dbSNP:558284287
873 873 a, g dbSNP:112573215
874 874 g, t dbSNP:781092468
876 876 a, c dbSNP:201706378
885 885 a, g dbSNP:370210752
896 896 a, g dbSNP:777915154
898 898 c, t dbSNP:142914598
899 899 a, c, t dbSNP:772231144
900 900 c, g dbSNP:747215797
901 901 c, t dbSNP:768765711
903 903 -, c dbSNP:776567343
903 903 c, g dbSNP:113722325
904 904 a, c dbSNP:151100443
906 906 a, c dbSNP:527753201
908 908 a, c, g dbSNP:200018992
909 909 a, c dbSNP:118054792
911 911 a, g dbSNP:762351343
928 928 c, t dbSNP:765867082
931 931 c, t dbSNP:141096005
934 934 a, c dbSNP:759159888
937 937 c, t dbSNP:767288617
939 939 c, g, t dbSNP:113309941
941 941 a, g dbSNP:756038747
946 946 c, t dbSNP:146876119
948 948 g, t dbSNP:1049311
949 949 -, ctggaccat dbSNP:765244722
949 949 c, t dbSNP:372896186
950 950 c, t dbSNP:757174659
953 953 a, g dbSNP:780114827
955 955 c, g dbSNP:777713368
963 963 c, t dbSNP:147958676
966 966 c, t dbSNP:768910663
967 967 a, g dbSNP:141506224
977 977 c, t dbSNP:748483350
985 985 c, g, t dbSNP:770197938
986 986 a, g dbSNP:755428861
988 988 c, t dbSNP:763473883
990 990 a, g dbSNP:377147669
991 991 c, g dbSNP:183059121
995 995 c, t dbSNP:61760973
997 997 -, tagtgt dbSNP:758373003
997 997 g, t dbSNP:767272326
1006 1006 a, g dbSNP:138205941
1011 1011 g, t dbSNP:752227276
1034 1034 c, t dbSNP:371579798
1035 1035 a, g dbSNP:375993336
1042 1042 a, g dbSNP:149176285
1045 1045 c, t dbSNP:769015094
1047 1047 c, t dbSNP:143131258
1048 1048 a, g dbSNP:763853325
1051 1051 a, g dbSNP:148234977
1053 1053 c, g dbSNP:776474910
1055 1055 c, t dbSNP:497582
1064 1064 a, g dbSNP:765193763
1069 1069 c, t dbSNP:750379822
1071 1071 a, c dbSNP:758358727
1076 1076 a, g dbSNP:767547817
1078 1078 c, g dbSNP:111270862
1086 1086 a, g dbSNP:146262415
1087 1087 c, g, t dbSNP:777912667
1089 1089 a, g dbSNP:757601482
1091 1091 a, g dbSNP:779450982
1107 1107 a, g dbSNP:746327927
1116 1116 g, t dbSNP:772736821
1124 1124 a, t dbSNP:564799362
1126 1126 a, g dbSNP:533564681
1154 1154 a, g dbSNP:36047324
1157 1157 c, g dbSNP:776188525
1159 1159 a, g dbSNP:761649691
1167 1167 a, g dbSNP:768103465
1169 1169 c, t dbSNP:780778970
1173 1173 c, g dbSNP:35560997
1176 1176 c, t dbSNP:769495018
1181 1181 a, g dbSNP:772929171
1187 1187 c, t dbSNP:570773289
1187 1187 -, t dbSNP:748631357
1191 1191 -, atc dbSNP:772751309
1201 1201 c, t dbSNP:150518427
1204 1204 c, t dbSNP:770848200
1210 1210 a, t dbSNP:372862365
1229 1229 a, g dbSNP:771477240
1231 1231 a, t dbSNP:556425857
1244 1244 c, t dbSNP:765526287
1245 1245 c, t dbSNP:139509954
1249 1249 a, g, t dbSNP:200866298
1252 1252 c, g dbSNP:377367930
1254 1254 c, g dbSNP:149705022
1256 1256 c, g dbSNP:755386042
1258 1258 a, g dbSNP:200189510
1265 1265 a, g dbSNP:781749544
1271 1271 a, g dbSNP:752140130
1272 1272 c, t dbSNP:755687648
1274 1274 a, t dbSNP:777380555
1290 1290 a, g dbSNP:180948326
1293 1293 a, g dbSNP:756932261
1297 1297 g, t dbSNP:201532817
1300 1300 c, g dbSNP:745688585
1306 1306 a, t dbSNP:17850649
1308 1308 a, g dbSNP:771899300
1311 1311 a, g dbSNP:201070810
1313 1313 -, ctta dbSNP:771202510
1322 1322 -, t dbSNP:776854797
1324 1324 c, t dbSNP:775537821
1325 1325 a, t dbSNP:748156460
1326 1326 a, g dbSNP:75430333
1335 1335 c, g dbSNP:202188706
1345 1345 c, g dbSNP:773459344
1355 1355 c, t dbSNP:763203759
1361 1361 a, g dbSNP:766725349
1385 1385 a, g dbSNP:200434738
1392 1392 a, g dbSNP:761101485
1394 1394 a, g dbSNP:764597215
1396 1396 c, t dbSNP:753333049
1401 1401 c, g dbSNP:756801221
1413 1413 g, t dbSNP:141752861
1422 1422 c, g dbSNP:750044524
1428 1428 c, t dbSNP:758020436
1430 1430 a, g dbSNP:779835379
1434 1434 a, g dbSNP:751327601
1436 1436 a, t dbSNP:148758785
1442 1442 g, t dbSNP:781350102
1453 1453 a, g dbSNP:745949756
1455 1455 a, t dbSNP:772326730
1456 1456 c, t dbSNP:780208101
1460 1460 a, g, t dbSNP:373894917
1463 1463 a, g dbSNP:533512853
1468 1468 g, t dbSNP:762238484
1470 1470 a, g dbSNP:368548573
1472 1472 c, t dbSNP:772761912
1473 1473 a, g dbSNP:762433166
1475 1475 c, t dbSNP:766019303
1476 1476 a, g dbSNP:371811647
1479 1479 c, t dbSNP:553438817
1483 1483 c, g dbSNP:375438296
1485 1485 a, g dbSNP:369989142
1489 1489 c, t dbSNP:756023441
1497 1497 c, g dbSNP:372175732
1519 1519 c, t dbSNP:750513249
1520 1520 a, g dbSNP:200276180
1521 1521 g, t dbSNP:374793216
1528 1528 a, g dbSNP:34398018
1530 1530 g, t dbSNP:147948716
1534 1534 a, g dbSNP:368909312
1548 1548 a, c, g dbSNP:748567027
1554 1554 c, g dbSNP:773627664
1561 1561 a, t dbSNP:748813806
1565 1565 a, g dbSNP:544330039
1579 1579 c, g, t dbSNP:141886009
1586 1586 a, g dbSNP:767292826
1587 1587 c, t dbSNP:775413853
1588 1588 a, g dbSNP:760454961
1596 1596 a, g dbSNP:764061627
1600 1600 c, t dbSNP:201928307
1606 1606 a, c dbSNP:757248125
1615 1615 c, g dbSNP:755241681
1623 1623 c, t dbSNP:766377898
1626 1626 c, g dbSNP:751649853
1636 1636 a, g dbSNP:755176882
1637 1637 c, t dbSNP:369787288
1647 1647 a, g dbSNP:748511132
1652 1652 a, g dbSNP:756480324
1654 1654 g, t dbSNP:778226421
1659 1659 a, g dbSNP:145345995
1680 1680 a, g dbSNP:771573054
1689 1689 a, g dbSNP:773943684
1692 1692 c, t dbSNP:374272120
1693 1693 a, g dbSNP:377638805
1695 1695 c, t dbSNP:775323800
1700 1700 c, t dbSNP:151049569
1703 1703 g, t dbSNP:763755572
1710 1710 -, aagat dbSNP:764087598
1710 1710 a, g dbSNP:546073588
1717 1717 g, t dbSNP:776489344
1730 1730 a, c, g dbSNP:74524673
1731 1731 a, g dbSNP:751631448
1737 1737 c, t dbSNP:376037131
1739 1739 c, g dbSNP:767700777
1747 1747 a, g dbSNP:752818327
1749 1749 g, t dbSNP:756393903
1750 1750 c, t dbSNP:777935430
1789 1789 -, t dbSNP:139648571
1817 1817 a, g dbSNP:76663700
1826 1826 a, g dbSNP:59918794
1876 1876 a, g dbSNP:75863418
1903 1903 c, t dbSNP:16926653
1935 1935 c, t dbSNP:550736007
1962 1962 g, t dbSNP:771515806
2034 2034 c, g dbSNP:555691861
2096 2096 a, g dbSNP:539067497
2189 2189 g, t dbSNP:9326
2190 2190 a, g dbSNP:567069522
2203 2203 c, t dbSNP:536022201
2211 2211 -, aata dbSNP:149231678
2218 2218 a, g dbSNP:369448429
2231 2231 a, c dbSNP:1063351
2247 2247 c, t dbSNP:575464356
2275 2275 a, g dbSNP:544287125
2298 2298 -, t dbSNP:562174425
2347 2347 a, c dbSNP:7808
2406 2406 a, g dbSNP:558824877
2412 2412 a, g dbSNP:762642480
2428 2428 a, g dbSNP:763837848
2438 2438 a, g dbSNP:3210067
2445 2445 c, t dbSNP:577566893
2446 2446 a, g dbSNP:577162977
2449 2449 c, t dbSNP:370785794
2465 2465 c, t dbSNP:3210068
2465 2465 c, t dbSNP:559562945
2486 2486 a, g dbSNP:751318367
2528 2528 a, g dbSNP:531264127
2542 2542 c, t dbSNP:956130
2548 2548 a, g dbSNP:757212170
2561 2561 c, t dbSNP:189194127
2567 2567 a, g dbSNP:542135463
2568 2568 a, t dbSNP:778661951
2593 2593 c, t dbSNP:1049333
2607 2607 g, t dbSNP:182283782

Target ORF information:

RefSeq Version NM_001135024
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001135024

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAATCAG GCGGCGCTGA GGGCAGCCCG GGGGCGGGGC GAACGGGGCG GGGGCCGGGG 
TCTGGTAAGG CCCCGCCGGC TGAGATATCC AGCGGCGCAC CTGACTTCCC GGGAGGTGAT
CCCATTAAGA TACTAATGCC ATCACTGTCT CCTACAATGG AAGAAGGAAA CATTGTGAAA
TGGCTGAAAA AGGAAGGTGA AGCGGTGAGT GCTGGAGATG CATTATGTGA AATTGAGACT
GACAAAGCTG TGGTTACCTT AGATGCAAGT GATGATGGAA TCTTGGCCAA AATCGTGGTT
GAAGAAGGAA GTAAAAATAT ACGGCTAGGT TCACTAATTG GTTTGATAGT AGAAGAAGGA
GAAGATTGGA AACATGTTGA AATTCCCAAA GACGTAGGTC CTCCACCACC AGTTTCAAAA
CCTTCAGAGC CTCGCCCCTC ACCAGAACCA CAGATTTCCA TCCCTGTCAA GAAGGAACAC
ATACCCGGGA CACTACGGTT CCGTTTAAGT CCAGCTGCCC GCAATATTCT GGAAAAACAC
TCACTGGATG CTAGCCAGGG CACAGCCACT GGCCCTCGGG GGATATTCAC TAAAGAGGAT
GCTCTCAAAC TTGTCCAGTT GAAACAAACG GGCAAGATTA CCGAGTCCAG ACCAACTCCA
GCCCCCACAG CCACTCCCAC AGCACCTTCG CCCCTACAGG CCACAGCTGG ACCATCTTAT
CCCCGGCCTG TGATCCCACC AGTATCAACT CCTGGACAAC CCAATGCAGT GGGCACATTC
ACTGAAATCC CCGCCAGCAA TATTCGAAGA GTTATTGCCA AGAGATTAAC TGAATCTAAA
AGTACTGTAC CTCATGCATA TGCTACTGCT GACTGTGACC TTGGAGCTGT TTTAAAAGTT
AGGCAAGATC TGGTCAAAGA TGACATTAAA GTATCAGTAA ATGATTTTAT CATCAAGGCA
GCAGCTGTTA CCCTTAAACA AATGCCAGAT GTTAATGTAA GCTGGGATGG AGAGGGCCCA
AAGCAACTGC CATTTATTGA CATTTCAGTG GCTGTGGCAA CAGATAAAGG CTTACTTACT
CCAATCATAA AAGATGCTGC TGCTAAAGGT ATCCAGGAAA TTGCTGACTC TGTAAAGGCT
CTATCAAAGA AAGCAAGAGA TGGAAAATTG TTGCCTGAAG AATACCAAGG AGGATCTTTT
AGTATTTCCA ACTTGGGGAT GTTTGGCATC GACGAATTTA CTGCAGTGAT TAACCCTCCT
CAGGCCTGCA TTTTGGCGGT TGGGAGGTTC CGACCTGTGC TGAAGCTCAC TGAGGATGAA
GAGGGAAATG CCAAACTGCA GCAGCGCCAG CTCATAACAG TCACAATGTC AAGTGACAGT
CGAGTGGTTG ATGACGAACT GGCAACCAGG TTTCTTAAAA GTTTTAAAGC AAACCTAGAG
AATCCTATCC GACTTGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10429
Accession Version NM_001166158.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 825bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase protein X component, mitochondrial isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107928.3, BC010389.1, AF001437.1 and AA282215.1. Summary: The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP). This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]. Transcript Variant: This variant (3) lacks multiple in-frame exons in the central coding region, compared to variant 1, resulting in a protein (isoform 3) that lacks 227 aa, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK301384.1, BU187130.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGCAAATGCC AGATGTTAAT
GTAAGCTGGG ATGGAGAGGG CCCAAAGCAA CTGCCATTTA TTGACATTTC AGTGGCTGTG
GCAACAGATA AAGGCTTACT TACTCCAATC ATAAAAGATG CTGCTGCTAA AGGTATCCAG
GAAATTGCTG ACTCTGTAAA GGCTCTATCA AAGAAAGCAA GAGATGGAAA ATTGTTGCCT
GAAGAATACC AAGGAGGATC TTTTAGTATT TCCAACTTGG GGATGTTTGG CATCGACGAA
TTTACTGCAG TGATTAACCC TCCTCAGGCC TGCATTTTGG CGGTTGGGAG GTTCCGACCT
GTGCTGAAGC TCACTGAGGA TGAAGAGGGA AATGCCAAAC TGCAGCAGCG CCAGCTCATA
ACAGTCACAA TGTCAAGTGA CAGTCGAGTG GTTGATGACG AACTGGCAAC CAGGTTTCTT
AAAAGTTTTA AAGCAAACCT AGAGAATCCT ATCCGACTTG CCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001159630.1
CDS527..1351
Misc Feature(1)344..346(+)
Misc Feature(2)692..1345(+)
Misc Feature(3)695..>868(+)
Misc Feature(4)785..835(+)
Misc Feature(5)815..817(+)
Misc Feature(6)851..1345(+)
Exon (1)1..686
Gene:PDHX
Gene Synonym:
Exon (2)687..767
Gene:PDHX
Gene Synonym:
Exon (3)768..868
Gene:PDHX
Gene Synonym:
Exon (4)869..1027
Gene:PDHX
Gene Synonym:
Exon (5)1028..1092
Gene:PDHX
Gene Synonym:
Exon (6)1093..2307
Gene:PDHX
Gene Synonym:
Translation
Position Chain Variation Link
5 5 a, c, g, t dbSNP:35924042
8 8 a, c dbSNP:561463212
21 21 ca, tg dbSNP:386752391
21 21 c, t dbSNP:2016814
22 22 a, g dbSNP:16926448
27 27 c, t dbSNP:563821039
37 37 c, t dbSNP:374157413
47 47 c, t dbSNP:375386462
48 48 a, g dbSNP:780056321
55 55 c, t dbSNP:552157217
61 61 c, t dbSNP:769982440
69 69 a, g dbSNP:773273824
75 75 c, g dbSNP:749560956
76 76 c, g dbSNP:771164728
81 81 c, t dbSNP:774655472
82 82 a, g dbSNP:759895240
94 94 c, t dbSNP:199893242
97 97 a, g dbSNP:202241286
99 99 c, t dbSNP:760074931
102 102 c, g dbSNP:763711108
105 105 a, g, t dbSNP:146420175
114 114 c, t dbSNP:756933277
116 116 a, g dbSNP:139123979
120 120 a, t dbSNP:369975644
124 124 c, t dbSNP:758274248
130 130 c, t dbSNP:779852629
134 134 -, c dbSNP:764336742
134 134 c, t dbSNP:747005576
137 137 a, g dbSNP:2956114
146 146 a, t dbSNP:777780994
148 148 a, c dbSNP:749401268
151 151 a, g dbSNP:771070943
156 156 g, t dbSNP:774565620
162 162 a, g dbSNP:746202661
164 164 c, t dbSNP:772530666
168 168 a, g dbSNP:775840738
173 173 c, t dbSNP:761199870
178 178 c, g dbSNP:764543470
185 185 a, g dbSNP:567329509
187 187 c, t dbSNP:761422179
190 190 c, g dbSNP:764971293
192 192 g, t dbSNP:750168506
196 196 a, g dbSNP:762622673
197 197 c, g dbSNP:766242750
198 198 a, c dbSNP:751333457
202 202 a, c, g dbSNP:753186558
206 206 a, c, g dbSNP:753853669
207 207 a, c, t dbSNP:779100145
208 208 a, g, t dbSNP:2956113
209 209 a, g dbSNP:747343062
212 212 a, c, t dbSNP:769228399
215 215 a, g dbSNP:761405893
219 219 c, t dbSNP:555955830
220 220 c, g dbSNP:772976341
221 221 a, g dbSNP:762615901
223 223 a, t dbSNP:765979127
225 225 a, g dbSNP:2956112
226 226 c, g dbSNP:759259296
227 227 a, g, t dbSNP:767425029
245 245 a, g dbSNP:757293776
250 250 c, g dbSNP:538595954
252 252 g, t dbSNP:750504158
255 255 a, g dbSNP:2956111
256 256 a, g, t dbSNP:780153979
270 270 c, t dbSNP:769211875
271 271 -, g dbSNP:752007834
278 278 a, g dbSNP:572118408
288 288 a, g dbSNP:756796889
319 319 c, t dbSNP:555380381
325 325 a, g dbSNP:113073242
327 327 a, g dbSNP:772737420
335 335 c, g dbSNP:574902434
340 340 c, g dbSNP:544017835
357 357 g, t dbSNP:770555008
362 362 a, g dbSNP:773929855
365 365 a, c dbSNP:759318998
370 370 c, t dbSNP:563586329
383 383 -, ggct dbSNP:762116656
386 386 c, t dbSNP:775289563
387 387 -, gggttgg dbSNP:767922429
390 390 g, t dbSNP:760569642
392 392 -, gt dbSNP:750683422
395 395 -, gggc dbSNP:756201233
395 395 a, g dbSNP:764026434
396 396 -, ggc dbSNP:139999735
396 396 -, gt dbSNP:754980282
397 397 -, gc dbSNP:753674694
398 398 -, cgg dbSNP:371074122
398 398 c, g, t dbSNP:112032820
399 399 -, ggggg dbSNP:778548879
403 403 c, g dbSNP:375130125
404 404 g, t dbSNP:758466385
428 428 a, t dbSNP:559322053
439 439 a, c dbSNP:2956110
460 460 c, g, t dbSNP:547276719
477 477 a, g dbSNP:766620539
480 480 a, c dbSNP:751771341
481 481 c, g, t dbSNP:764604412
484 484 a, c dbSNP:781647178
486 486 a, c, g dbSNP:748553521
487 487 c, g, t dbSNP:369228797
492 492 a, c, g dbSNP:372814513
494 494 a, g dbSNP:774021266
500 500 -, g dbSNP:747994483
500 500 c, t dbSNP:529801753
501 501 a, g, t dbSNP:377596373
503 503 c, g dbSNP:760481755
504 504 a, c dbSNP:201033364
505 505 a, c dbSNP:776499519
506 506 a, g dbSNP:763059743
511 511 g, t dbSNP:569788862
519 519 c, t dbSNP:766421119
520 520 c, t dbSNP:751680964
523 523 c, g dbSNP:3818401
528 528 c, t dbSNP:558713973
531 531 c, g dbSNP:753053135
532 532 a, g dbSNP:756600157
535 535 c, t dbSNP:778335059
539 539 g, t dbSNP:749796976
545 545 c, g dbSNP:147403982
548 548 g, t dbSNP:778248643
549 549 c, g dbSNP:374254725
555 555 a, c dbSNP:139613834
561 561 c, g dbSNP:200419151
564 564 c, t dbSNP:775185079
570 570 a, g dbSNP:387906998
573 573 a, c dbSNP:118136428
575 575 c, t dbSNP:182569882
581 581 g, t dbSNP:761609180
586 586 a, c dbSNP:769800412
588 588 c, t dbSNP:774504089
589 589 c, t dbSNP:2956109
593 593 c, t dbSNP:1049306
594 594 a, g dbSNP:753030741
596 596 c, g dbSNP:11539201
597 597 a, g dbSNP:764412516
608 608 c, t dbSNP:754249799
613 613 -, gaag dbSNP:724159828
615 615 a, g dbSNP:757640533
616 616 a, g, t dbSNP:374923275
619 619 g, t dbSNP:377527030
623 623 c, t dbSNP:757970464
626 626 c, g dbSNP:779650616
629 629 a, t dbSNP:746715011
634 634 c, t dbSNP:1049307
635 635 g, t dbSNP:780807263
642 642 c, g dbSNP:747977950
643 643 c, t dbSNP:769559458
644 644 a, g dbSNP:773340684
655 655 g, t dbSNP:759679470
681 681 a, g, t dbSNP:772315120
686 686 a, g dbSNP:146678221
687 687 a, g dbSNP:771011394
704 704 c, g dbSNP:775669398
706 706 a, g dbSNP:747128117
712 712 a, g dbSNP:768751336
717 717 g, t dbSNP:776981891
720 720 c, g dbSNP:762057004
728 728 a, g dbSNP:765643071
729 729 c, t dbSNP:773591910
740 740 a, c dbSNP:143301712
751 751 a, g dbSNP:574905086
763 763 a, g dbSNP:750973777
768 768 a, g dbSNP:554051809
771 771 a, t dbSNP:778685760
774 774 c, t dbSNP:745866603
775 775 a, g dbSNP:376628638
776 776 a, g dbSNP:200562821
780 780 g, t dbSNP:748233840
791 791 a, g dbSNP:139199177
793 793 a, g dbSNP:111829480
796 796 a, t dbSNP:778024968
808 808 a, g dbSNP:749644848
812 812 a, g dbSNP:771320500
821 821 c, g, t dbSNP:373211545
827 827 a, g dbSNP:11539202
828 828 c, t dbSNP:61752924
834 834 a, g dbSNP:566746801
843 843 a, g dbSNP:146456454
845 845 a, g dbSNP:72912954
847 847 a, t dbSNP:201359586
851 851 a, t dbSNP:748447623
855 855 c, t dbSNP:753585930
862 862 -, aatc dbSNP:752635475
865 865 c, t dbSNP:34582941
866 866 a, g dbSNP:765036658
877 877 a, g dbSNP:771477240
879 879 a, t dbSNP:556425857
892 892 c, t dbSNP:765526287
893 893 c, t dbSNP:139509954
897 897 a, g, t dbSNP:200866298
900 900 c, g dbSNP:377367930
902 902 c, g dbSNP:149705022
904 904 c, g dbSNP:755386042
906 906 a, g dbSNP:200189510
913 913 a, g dbSNP:781749544
919 919 a, g dbSNP:752140130
920 920 c, t dbSNP:755687648
922 922 a, t dbSNP:777380555
938 938 a, g dbSNP:180948326
941 941 a, g dbSNP:756932261
945 945 g, t dbSNP:201532817
948 948 c, g dbSNP:745688585
954 954 a, t dbSNP:17850649
956 956 a, g dbSNP:771899300
959 959 a, g dbSNP:201070810
961 961 -, ctta dbSNP:771202510
970 970 -, t dbSNP:776854797
972 972 c, t dbSNP:775537821
973 973 a, t dbSNP:748156460
974 974 a, g dbSNP:75430333
983 983 c, g dbSNP:202188706
993 993 c, g dbSNP:773459344
1003 1003 c, t dbSNP:763203759
1009 1009 a, g dbSNP:766725349
1033 1033 a, g dbSNP:200434738
1040 1040 a, g dbSNP:761101485
1042 1042 a, g dbSNP:764597215
1044 1044 c, t dbSNP:753333049
1049 1049 c, g dbSNP:756801221
1061 1061 g, t dbSNP:141752861
1070 1070 c, g dbSNP:750044524
1076 1076 c, t dbSNP:758020436
1078 1078 a, g dbSNP:779835379
1082 1082 a, g dbSNP:751327601
1084 1084 a, t dbSNP:148758785
1090 1090 g, t dbSNP:781350102
1101 1101 a, g dbSNP:745949756
1103 1103 a, t dbSNP:772326730
1104 1104 c, t dbSNP:780208101
1108 1108 a, g, t dbSNP:373894917
1111 1111 a, g dbSNP:533512853
1116 1116 g, t dbSNP:762238484
1118 1118 a, g dbSNP:368548573
1120 1120 c, t dbSNP:772761912
1121 1121 a, g dbSNP:762433166
1123 1123 c, t dbSNP:766019303
1124 1124 a, g dbSNP:371811647
1127 1127 c, t dbSNP:553438817
1131 1131 c, g dbSNP:375438296
1133 1133 a, g dbSNP:369989142
1137 1137 c, t dbSNP:756023441
1145 1145 c, g dbSNP:372175732
1167 1167 c, t dbSNP:750513249
1168 1168 a, g dbSNP:200276180
1169 1169 g, t dbSNP:374793216
1176 1176 a, g dbSNP:34398018
1178 1178 g, t dbSNP:147948716
1182 1182 a, g dbSNP:368909312
1196 1196 a, c, g dbSNP:748567027
1202 1202 c, g dbSNP:773627664
1209 1209 a, t dbSNP:748813806
1213 1213 a, g dbSNP:544330039
1227 1227 c, g, t dbSNP:141886009
1234 1234 a, g dbSNP:767292826
1235 1235 c, t dbSNP:775413853
1236 1236 a, g dbSNP:760454961
1244 1244 a, g dbSNP:764061627
1248 1248 c, t dbSNP:201928307
1254 1254 a, c dbSNP:757248125
1263 1263 c, g dbSNP:755241681
1271 1271 c, t dbSNP:766377898
1274 1274 c, g dbSNP:751649853
1284 1284 a, g dbSNP:755176882
1285 1285 c, t dbSNP:369787288
1295 1295 a, g dbSNP:748511132
1300 1300 a, g dbSNP:756480324
1302 1302 g, t dbSNP:778226421
1307 1307 a, g dbSNP:145345995
1328 1328 a, g dbSNP:771573054
1337 1337 a, g dbSNP:773943684
1340 1340 c, t dbSNP:374272120
1341 1341 a, g dbSNP:377638805
1343 1343 c, t dbSNP:775323800
1348 1348 c, t dbSNP:151049569
1351 1351 g, t dbSNP:763755572
1358 1358 -, aagat dbSNP:764087598
1358 1358 a, g dbSNP:546073588
1365 1365 g, t dbSNP:776489344
1378 1378 a, c, g dbSNP:74524673
1379 1379 a, g dbSNP:751631448
1385 1385 c, t dbSNP:376037131
1387 1387 c, g dbSNP:767700777
1395 1395 a, g dbSNP:752818327
1397 1397 g, t dbSNP:756393903
1398 1398 c, t dbSNP:777935430
1437 1437 -, t dbSNP:139648571
1465 1465 a, g dbSNP:76663700
1474 1474 a, g dbSNP:59918794
1524 1524 a, g dbSNP:75863418
1551 1551 c, t dbSNP:16926653
1583 1583 c, t dbSNP:550736007
1610 1610 g, t dbSNP:771515806
1682 1682 c, g dbSNP:555691861
1744 1744 a, g dbSNP:539067497
1837 1837 g, t dbSNP:9326
1838 1838 a, g dbSNP:567069522
1851 1851 c, t dbSNP:536022201
1859 1859 -, aata dbSNP:149231678
1866 1866 a, g dbSNP:369448429
1879 1879 a, c dbSNP:1063351
1895 1895 c, t dbSNP:575464356
1923 1923 a, g dbSNP:544287125
1946 1946 -, t dbSNP:562174425
1995 1995 a, c dbSNP:7808
2054 2054 a, g dbSNP:558824877
2060 2060 a, g dbSNP:762642480
2076 2076 a, g dbSNP:763837848
2086 2086 a, g dbSNP:3210067
2093 2093 c, t dbSNP:577566893
2094 2094 a, g dbSNP:577162977
2097 2097 c, t dbSNP:370785794
2113 2113 c, t dbSNP:3210068
2113 2113 c, t dbSNP:559562945
2134 2134 a, g dbSNP:751318367
2176 2176 a, g dbSNP:531264127
2190 2190 c, t dbSNP:956130
2196 2196 a, g dbSNP:757212170
2209 2209 c, t dbSNP:189194127
2215 2215 a, g dbSNP:542135463
2216 2216 a, t dbSNP:778661951
2241 2241 c, t dbSNP:1049333
2255 2255 g, t dbSNP:182283782

Target ORF information:

RefSeq Version NM_001166158
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166158

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGCAAATGCC AGATGTTAAT
GTAAGCTGGG ATGGAGAGGG CCCAAAGCAA CTGCCATTTA TTGACATTTC AGTGGCTGTG
GCAACAGATA AAGGCTTACT TACTCCAATC ATAAAAGATG CTGCTGCTAA AGGTATCCAG
GAAATTGCTG ACTCTGTAAA GGCTCTATCA AAGAAAGCAA GAGATGGAAA ATTGTTGCCT
GAAGAATACC AAGGAGGATC TTTTAGTATT TCCAACTTGG GGATGTTTGG CATCGACGAA
TTTACTGCAG TGATTAACCC TCCTCAGGCC TGCATTTTGG CGGTTGGGAG GTTCCGACCT
GTGCTGAAGC TCACTGAGGA TGAAGAGGGA AATGCCAAAC TGCAGCAGCG CCAGCTCATA
ACAGTCACAA TGTCAAGTGA CAGTCGAGTG GTTGATGACG AACTGGCAAC CAGGTTTCTT
AAAAGTTTTA AAGCAAACCT AGAGAATCCT ATCCGACTTG CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.