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PDHX cDNA ORF clone, Homo sapiens (human)

Gene Symbol PDHX
Entrez Gene ID 8050
Full Name pyruvate dehydrogenase complex, component X
Synonyms DLDBP, E3BP, OPDX, PDX1, proX
General protein information
Preferred Names
pyruvate dehydrogenase protein X component, mitochondrial
Names
pyruvate dehydrogenase protein X component, mitochondrial
pyruvate dehydrogenase complex, E3-binding protein subunit
lipoyl-containing pyruvate dehydrogenase complex component X
pyruvate dehydrogenase complex, lipoyl-containing component X
dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p13

Summary The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP). This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]. lac of sum
Disorder MIM:

608769

Disorder Html: Lacticacidemia due to PDX1 deficiency, 245349 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011520390 XP_011518692 pyruvate dehydrogenase protein X component, mitochondrial isoform X1
NM_003477 NP_003468 pyruvate dehydrogenase protein X component, mitochondrial isoform 1 precursor
NM_001135024 NP_001128496 pyruvate dehydrogenase protein X component, mitochondrial isoform 2
NM_001166158 NP_001159630 pyruvate dehydrogenase protein X component, mitochondrial isoform 3 precursor



Homo sapiens (human) PDHX NP_003468.2
Pan troglodytes (chimpanzee) PDHX XP_001149489.1
Macaca mulatta (Rhesus monkey) PDHX XP_001109997.2
Canis lupus familiaris (dog) PDHX XP_533153.2
Bos taurus (cattle) LOC517402 NP_001069219.1
Mus musculus (house mouse) Pdhx NP_780303.1
Gallus gallus (chicken) PDHX NP_001026358.1
Danio rerio (zebrafish) pdhx NP_956854.1
Xenopus (Silurana) tropicalis (western clawed frog) pdhx XP_002937341.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PDHX gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDHX cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu61608 XM_011520390 PREDICTED: Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu19013 NM_003477 Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu07230 NM_001135024 Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $286.30
$409.00
OHu10429 NM_001166158 Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu61608
Clone ID Related Accession (Same CDS sequence) XM_011520390
Accession Version XM_011520390.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1326bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase protein X component, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA 
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT
GCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518692.1
CDS131..1456
Misc Feature(1)131..1450(+)
Misc Feature(2)131..343(+)
Misc Feature(3)209..259(+)
Misc Feature(4)239..241(+)
Misc Feature(5)491..604(+)
Misc Feature(6)821..1450(+)
Translation

Target ORF information:

RefSeq Version XM_011520390
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520390

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA 
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT
GCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19013
Clone ID Related Accession (Same CDS sequence) NM_003477
Accession Version NM_003477.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1506bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase protein X component, mitochondrial isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107928.3, BC010389.1, AF001437.1 and AA282215.1. This sequence is a reference standard in the RefSeqGene project. On Sep 17, 2008 this sequence version replaced gi:4505698. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BX648910.1, U82328.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT
GCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003468.2
CDS527..2032
Misc Feature(1)344..346(+)
Misc Feature(2)695..919(+)
Misc Feature(3)698..2026(+)
Misc Feature(4)785..835(+)
Misc Feature(5)815..817(+)
Misc Feature(6)1067..1180(+)
Misc Feature(7)1073..1168(+)
Misc Feature(8)1106..1108(+)
Misc Feature(9)1397..2026(+)
Exon (1)1..686
Gene:PDHX
Gene Synonym:
Exon (2)687..767
Gene:PDHX
Gene Synonym:
Exon (3)768..868
Gene:PDHX
Gene Synonym:
Exon (4)869..1068
Gene:PDHX
Gene Synonym:
Exon (5)1069..1167
Gene:PDHX
Gene Synonym:
Exon (6)1168..1342
Gene:PDHX
Gene Synonym:
Exon (7)1343..1490
Gene:PDHX
Gene Synonym:
Exon (8)1491..1549
Gene:PDHX
Gene Synonym:
Exon (9)1550..1708
Gene:PDHX
Gene Synonym:
Exon (10)1709..1773
Gene:PDHX
Gene Synonym:
Exon (11)1774..2988
Gene:PDHX
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003477
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003477

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGGTTGAAGA AGGAAGTAAA
AATATACGGC TAGGTTCACT AATTGGTTTG ATAGTAGAAG AAGGAGAAGA TTGGAAACAT
GTTGAAATTC CCAAAGACGT AGGTCCTCCA CCACCAGTTT CAAAACCTTC AGAGCCTCGC
CCCTCACCAG AACCACAGAT TTCCATCCCT GTCAAGAAGG AACACATACC CGGGACACTA
CGGTTCCGTT TAAGTCCAGC TGCCCGCAAT ATTCTGGAAA AACACTCACT GGATGCTAGC
CAGGGCACAG CCACTGGCCC TCGGGGGATA TTCACTAAAG AGGATGCTCT CAAACTTGTC
CAGTTGAAAC AAACGGGCAA GATTACCGAG TCCAGACCAA CTCCAGCCCC CACAGCCACT
CCCACAGCAC CTTCGCCCCT ACAGGCCACA GCTGGACCAT CTTATCCCCG GCCTGTGATC
CCACCAGTAT CAACTCCTGG ACAACCCAAT GCAGTGGGCA CATTCACTGA AATCCCCGCC
AGCAATATTC GAAGAGTTAT TGCCAAGAGA TTAACTGAAT CTAAAAGTAC TGTACCTCAT
GCATATGCTA CTGCTGACTG TGACCTTGGA GCTGTTTTAA AAGTTAGGCA AGATCTGGTC
AAAGATGACA TTAAAGTATC AGTAAATGAT TTTATCATCA AGGCAGCAGC TGTTACCCTT
AAACAAATGC CAGATGTTAA TGTAAGCTGG GATGGAGAGG GCCCAAAGCA ACTGCCATTT
ATTGACATTT CAGTGGCTGT GGCAACAGAT AAAGGCTTAC TTACTCCAAT CATAAAAGAT
GCTGCTGCTA AAGGTATCCA GGAAATTGCT GACTCTGTAA AGGCTCTATC AAAGAAAGCA
AGAGATGGAA AATTGTTGCC TGAAGAATAC CAAGGAGGAT CTTTTAGTAT TTCCAACTTG
GGGATGTTTG GCATCGACGA ATTTACTGCA GTGATTAACC CTCCTCAGGC CTGCATTTTG
GCGGTTGGGA GGTTCCGACC TGTGCTGAAG CTCACTGAGG ATGAAGAGGG AAATGCCAAA
CTGCAGCAGC GCCAGCTCAT AACAGTCACA ATGTCAAGTG ACAGTCGAGT GGTTGATGAC
GAACTGGCAA CCAGGTTTCT TAAAAGTTTT AAAGCAAACC TAGAGAATCC TATCCGACTT
GCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu07230
Clone ID Related Accession (Same CDS sequence) NM_001135024
Accession Version NM_001135024.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase protein X component, mitochondrial isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA573433.1, BI915296.1, U79296.1 and AA282215.1. Transcript Variant: This variant (2) lacks a segment in the 5' region, resulting in upstream in-frame AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAATCAG GCGGCGCTGA GGGCAGCCCG GGGGCGGGGC GAACGGGGCG GGGGCCGGGG 
TCTGGTAAGG CCCCGCCGGC TGAGATATCC AGCGGCGCAC CTGACTTCCC GGGAGGTGAT
CCCATTAAGA TACTAATGCC ATCACTGTCT CCTACAATGG AAGAAGGAAA CATTGTGAAA
TGGCTGAAAA AGGAAGGTGA AGCGGTGAGT GCTGGAGATG CATTATGTGA AATTGAGACT
GACAAAGCTG TGGTTACCTT AGATGCAAGT GATGATGGAA TCTTGGCCAA AATCGTGGTT
GAAGAAGGAA GTAAAAATAT ACGGCTAGGT TCACTAATTG GTTTGATAGT AGAAGAAGGA
GAAGATTGGA AACATGTTGA AATTCCCAAA GACGTAGGTC CTCCACCACC AGTTTCAAAA
CCTTCAGAGC CTCGCCCCTC ACCAGAACCA CAGATTTCCA TCCCTGTCAA GAAGGAACAC
ATACCCGGGA CACTACGGTT CCGTTTAAGT CCAGCTGCCC GCAATATTCT GGAAAAACAC
TCACTGGATG CTAGCCAGGG CACAGCCACT GGCCCTCGGG GGATATTCAC TAAAGAGGAT
GCTCTCAAAC TTGTCCAGTT GAAACAAACG GGCAAGATTA CCGAGTCCAG ACCAACTCCA
GCCCCCACAG CCACTCCCAC AGCACCTTCG CCCCTACAGG CCACAGCTGG ACCATCTTAT
CCCCGGCCTG TGATCCCACC AGTATCAACT CCTGGACAAC CCAATGCAGT GGGCACATTC
ACTGAAATCC CCGCCAGCAA TATTCGAAGA GTTATTGCCA AGAGATTAAC TGAATCTAAA
AGTACTGTAC CTCATGCATA TGCTACTGCT GACTGTGACC TTGGAGCTGT TTTAAAAGTT
AGGCAAGATC TGGTCAAAGA TGACATTAAA GTATCAGTAA ATGATTTTAT CATCAAGGCA
GCAGCTGTTA CCCTTAAACA AATGCCAGAT GTTAATGTAA GCTGGGATGG AGAGGGCCCA
AAGCAACTGC CATTTATTGA CATTTCAGTG GCTGTGGCAA CAGATAAAGG CTTACTTACT
CCAATCATAA AAGATGCTGC TGCTAAAGGT ATCCAGGAAA TTGCTGACTC TGTAAAGGCT
CTATCAAAGA AAGCAAGAGA TGGAAAATTG TTGCCTGAAG AATACCAAGG AGGATCTTTT
AGTATTTCCA ACTTGGGGAT GTTTGGCATC GACGAATTTA CTGCAGTGAT TAACCCTCCT
CAGGCCTGCA TTTTGGCGGT TGGGAGGTTC CGACCTGTGC TGAAGCTCAC TGAGGATGAA
GAGGGAAATG CCAAACTGCA GCAGCGCCAG CTCATAACAG TCACAATGTC AAGTGACAGT
CGAGTGGTTG ATGACGAACT GGCAACCAGG TTTCTTAAAA GTTTTAAAGC AAACCTAGAG
AATCCTATCC GACTTGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001128496.1
CDS243..1703
Misc Feature(1)366..590(+)
Misc Feature(2)369..1697(+)
Misc Feature(3)456..506(+)
Misc Feature(4)486..488(+)
Misc Feature(5)738..851(+)
Misc Feature(6)1068..1697(+)
Exon (1)1..357
Gene:PDHX
Gene Synonym:
Exon (2)358..438
Gene:PDHX
Gene Synonym:
Exon (3)439..539
Gene:PDHX
Gene Synonym:
Exon (4)540..739
Gene:PDHX
Gene Synonym:
Exon (5)740..838
Gene:PDHX
Gene Synonym:
Exon (6)839..1013
Gene:PDHX
Gene Synonym:
Exon (7)1014..1161
Gene:PDHX
Gene Synonym:
Exon (8)1162..1220
Gene:PDHX
Gene Synonym:
Exon (9)1221..1379
Gene:PDHX
Gene Synonym:
Exon (10)1380..1444
Gene:PDHX
Gene Synonym:
Exon (11)1445..2659
Gene:PDHX
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001135024
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001135024

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAATCAG GCGGCGCTGA GGGCAGCCCG GGGGCGGGGC GAACGGGGCG GGGGCCGGGG 
TCTGGTAAGG CCCCGCCGGC TGAGATATCC AGCGGCGCAC CTGACTTCCC GGGAGGTGAT
CCCATTAAGA TACTAATGCC ATCACTGTCT CCTACAATGG AAGAAGGAAA CATTGTGAAA
TGGCTGAAAA AGGAAGGTGA AGCGGTGAGT GCTGGAGATG CATTATGTGA AATTGAGACT
GACAAAGCTG TGGTTACCTT AGATGCAAGT GATGATGGAA TCTTGGCCAA AATCGTGGTT
GAAGAAGGAA GTAAAAATAT ACGGCTAGGT TCACTAATTG GTTTGATAGT AGAAGAAGGA
GAAGATTGGA AACATGTTGA AATTCCCAAA GACGTAGGTC CTCCACCACC AGTTTCAAAA
CCTTCAGAGC CTCGCCCCTC ACCAGAACCA CAGATTTCCA TCCCTGTCAA GAAGGAACAC
ATACCCGGGA CACTACGGTT CCGTTTAAGT CCAGCTGCCC GCAATATTCT GGAAAAACAC
TCACTGGATG CTAGCCAGGG CACAGCCACT GGCCCTCGGG GGATATTCAC TAAAGAGGAT
GCTCTCAAAC TTGTCCAGTT GAAACAAACG GGCAAGATTA CCGAGTCCAG ACCAACTCCA
GCCCCCACAG CCACTCCCAC AGCACCTTCG CCCCTACAGG CCACAGCTGG ACCATCTTAT
CCCCGGCCTG TGATCCCACC AGTATCAACT CCTGGACAAC CCAATGCAGT GGGCACATTC
ACTGAAATCC CCGCCAGCAA TATTCGAAGA GTTATTGCCA AGAGATTAAC TGAATCTAAA
AGTACTGTAC CTCATGCATA TGCTACTGCT GACTGTGACC TTGGAGCTGT TTTAAAAGTT
AGGCAAGATC TGGTCAAAGA TGACATTAAA GTATCAGTAA ATGATTTTAT CATCAAGGCA
GCAGCTGTTA CCCTTAAACA AATGCCAGAT GTTAATGTAA GCTGGGATGG AGAGGGCCCA
AAGCAACTGC CATTTATTGA CATTTCAGTG GCTGTGGCAA CAGATAAAGG CTTACTTACT
CCAATCATAA AAGATGCTGC TGCTAAAGGT ATCCAGGAAA TTGCTGACTC TGTAAAGGCT
CTATCAAAGA AAGCAAGAGA TGGAAAATTG TTGCCTGAAG AATACCAAGG AGGATCTTTT
AGTATTTCCA ACTTGGGGAT GTTTGGCATC GACGAATTTA CTGCAGTGAT TAACCCTCCT
CAGGCCTGCA TTTTGGCGGT TGGGAGGTTC CGACCTGTGC TGAAGCTCAC TGAGGATGAA
GAGGGAAATG CCAAACTGCA GCAGCGCCAG CTCATAACAG TCACAATGTC AAGTGACAGT
CGAGTGGTTG ATGACGAACT GGCAACCAGG TTTCTTAAAA GTTTTAAAGC AAACCTAGAG
AATCCTATCC GACTTGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10429
Clone ID Related Accession (Same CDS sequence) NM_001166158
Accession Version NM_001166158.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 825bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pyruvate dehydrogenase protein X component, mitochondrial isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107928.3, BC010389.1, AF001437.1 and AA282215.1. Transcript Variant: This variant (3) lacks multiple in-frame exons in the central coding region, compared to variant 1, resulting in a protein (isoform 3) that lacks 227 aa, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK301384.1, BU187130.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGCAAATGCC AGATGTTAAT
GTAAGCTGGG ATGGAGAGGG CCCAAAGCAA CTGCCATTTA TTGACATTTC AGTGGCTGTG
GCAACAGATA AAGGCTTACT TACTCCAATC ATAAAAGATG CTGCTGCTAA AGGTATCCAG
GAAATTGCTG ACTCTGTAAA GGCTCTATCA AAGAAAGCAA GAGATGGAAA ATTGTTGCCT
GAAGAATACC AAGGAGGATC TTTTAGTATT TCCAACTTGG GGATGTTTGG CATCGACGAA
TTTACTGCAG TGATTAACCC TCCTCAGGCC TGCATTTTGG CGGTTGGGAG GTTCCGACCT
GTGCTGAAGC TCACTGAGGA TGAAGAGGGA AATGCCAAAC TGCAGCAGCG CCAGCTCATA
ACAGTCACAA TGTCAAGTGA CAGTCGAGTG GTTGATGACG AACTGGCAAC CAGGTTTCTT
AAAAGTTTTA AAGCAAACCT AGAGAATCCT ATCCGACTTG CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001159630.1
CDS527..1351
Misc Feature(1)344..346(+)
Misc Feature(2)692..1345(+)
Misc Feature(3)695..>868(+)
Misc Feature(4)785..835(+)
Misc Feature(5)815..817(+)
Misc Feature(6)851..1345(+)
Exon (1)1..686
Gene:PDHX
Gene Synonym:
Exon (2)687..767
Gene:PDHX
Gene Synonym:
Exon (3)768..868
Gene:PDHX
Gene Synonym:
Exon (4)869..1027
Gene:PDHX
Gene Synonym:
Exon (5)1028..1092
Gene:PDHX
Gene Synonym:
Exon (6)1093..2307
Gene:PDHX
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001166158
Organism Homo sapiens (human)
Definition Homo sapiens pyruvate dehydrogenase complex, component X (PDHX), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166158

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCGGCCT CCTGGAGGCT GGGCTGTGAT CCGCGGCTGC TGCGTTATCT TGTGGGCTTC 
CCCGGCCGCC GAAGCGTAGG GCTGGTGAAG GGGGCTCTTG GGTGGTCTGT AAGCCGCGGA
GCTAATTGGA GATGGTTTCA CAGCACGCAG TGGCTTCGGG GTGATCCCAT TAAGATACTA
ATGCCATCAC TGTCTCCTAC AATGGAAGAA GGAAACATTG TGAAATGGCT GAAAAAGGAA
GGTGAAGCGG TGAGTGCTGG AGATGCATTA TGTGAAATTG AGACTGACAA AGCTGTGGTT
ACCTTAGATG CAAGTGATGA TGGAATCTTG GCCAAAATCG TGCAAATGCC AGATGTTAAT
GTAAGCTGGG ATGGAGAGGG CCCAAAGCAA CTGCCATTTA TTGACATTTC AGTGGCTGTG
GCAACAGATA AAGGCTTACT TACTCCAATC ATAAAAGATG CTGCTGCTAA AGGTATCCAG
GAAATTGCTG ACTCTGTAAA GGCTCTATCA AAGAAAGCAA GAGATGGAAA ATTGTTGCCT
GAAGAATACC AAGGAGGATC TTTTAGTATT TCCAACTTGG GGATGTTTGG CATCGACGAA
TTTACTGCAG TGATTAACCC TCCTCAGGCC TGCATTTTGG CGGTTGGGAG GTTCCGACCT
GTGCTGAAGC TCACTGAGGA TGAAGAGGGA AATGCCAAAC TGCAGCAGCG CCAGCTCATA
ACAGTCACAA TGTCAAGTGA CAGTCGAGTG GTTGATGACG AACTGGCAAC CAGGTTTCTT
AAAAGTTTTA AAGCAAACCT AGAGAATCCT ATCCGACTTG CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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