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COL18A1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol COL18A1
Entrez Gene ID 80781
Full Name collagen, type XVIII, alpha 1
Synonyms KNO, KNO1, KS
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

21

21q22.3

Summary This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]. lac of sum
Disorder MIM:

120328

Disorder Html: Knobloch syndrome, type 1, 267750 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_130445 NP_569712 collagen alpha-1(XVIII) chain isoform 2 preproprotein
NM_030582 NP_085059 collagen alpha-1(XVIII) chain isoform 1 preproprotein
NM_130444 NP_569711 collagen alpha-1(XVIII) chain isoform 3 preproprotein



Homo sapiens (human) COL18A1 NP_085059.2
Pan troglodytes (chimpanzee) COL18A1 XP_531500.4
Macaca mulatta (Rhesus monkey) COL18A1 XP_002803149.1
Mus musculus (house mouse) Col18a1 NP_001103461.1
Rattus norvegicus (Norway rat) Col18a1 NP_445941.2
Gallus gallus (chicken) COL18A1 NP_989495.1
Xenopus (Silurana) tropicalis (western clawed frog) col18a1 NP_989236.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following COL18A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL18A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19786 NM_130445 Homo sapiens collagen, type XVIII, alpha 1 (COL18A1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $384.30
$549.00
OHu19882 NM_030582 Homo sapiens collagen, type XVIII, alpha 1 (COL18A1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $923.30
$1319.00
OHu72836 NM_130444 Homo sapiens collagen, type XVIII, alpha 1 (COL18A1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $951.30
$1359.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19786
Clone ID Related Accession (Same CDS sequence) NM_130445
Accession Version NM_130445.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-1(XVIII) chain isoform 2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN389577.1, BC063833.1 and AF018082.1. On Dec 14, 2014 this sequence version replaced gi:110611232. Transcript Variant: This variant (2, also known as NCI-303) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 3. The encoded isoform (2) has a distinct and shorter N-terminus compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018082.1, BC063833.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGCGCCGA GGTGCCCCTG GCCATGGCCG CGGCGGCGGC GCCTCCTGGA CGTGCTCGCG 
CCCCTGGTCC TGCTGCTCGG GGTCCGCGCG GCCTCCGCGG AGCCAGAGCG CATCAGCGAG
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_569712.2
CDS95..4105
Misc Feature(1)215..781(+)
Misc Feature(2)1304..1417(+)
Misc Feature(3)2018..2170(+)
Misc Feature(4)2270..2392(+)
Misc Feature(5)3158..4102(+)
Misc Feature(6)3572..4084(+)
Misc Feature(7)3665..3970(+)
Exon (1)1..105
Gene:COL18A1
Gene Synonym:
Exon (2)106..200
Gene:COL18A1
Gene Synonym:
Exon (3)201..745
Gene:COL18A1
Gene Synonym:
Exon (4)746..832
Gene:COL18A1
Gene Synonym:
Exon (5)833..892
Gene:COL18A1
Gene Synonym:
Exon (6)893..1022
Gene:COL18A1
Gene Synonym:
Exon (7)1023..1099
Gene:COL18A1
Gene Synonym:
Exon (8)1100..1315
Gene:COL18A1
Gene Synonym:
Exon (9)1316..1342
Gene:COL18A1
Gene Synonym:
Exon (10)1343..1405
Gene:COL18A1
Gene Synonym:
Exon (11)1406..1492
Gene:COL18A1
Gene Synonym:
Exon (12)1493..1546
Gene:COL18A1
Gene Synonym:
Exon (13)1547..1705
Gene:COL18A1
Gene Synonym:
Exon (14)1706..1768
Gene:COL18A1
Gene Synonym:
Exon (15)1769..1795
Gene:COL18A1
Gene Synonym:
Exon (16)1796..1927
Gene:COL18A1
Gene Synonym:
Exon (17)1928..1990
Gene:COL18A1
Gene Synonym:
Exon (18)1991..2017
Gene:COL18A1
Gene Synonym:
Exon (19)2018..2053
Gene:COL18A1
Gene Synonym:
Exon (20)2054..2125
Gene:COL18A1
Gene Synonym:
Exon (21)2126..2161
Gene:COL18A1
Gene Synonym:
Exon (22)2162..2251
Gene:COL18A1
Gene Synonym:
Exon (23)2252..2281
Gene:COL18A1
Gene Synonym:
Exon (24)2282..2308
Gene:COL18A1
Gene Synonym:
Exon (25)2309..2371
Gene:COL18A1
Gene Synonym:
Exon (26)2372..2446
Gene:COL18A1
Gene Synonym:
Exon (27)2447..2473
Gene:COL18A1
Gene Synonym:
Exon (28)2474..2527
Gene:COL18A1
Gene Synonym:
Exon (29)2528..2602
Gene:COL18A1
Gene Synonym:
Exon (30)2603..2671
Gene:COL18A1
Gene Synonym:
Exon (31)2672..2714
Gene:COL18A1
Gene Synonym:
Exon (32)2715..2777
Gene:COL18A1
Gene Synonym:
Exon (33)2778..2821
Gene:COL18A1
Gene Synonym:
Exon (34)2822..2953
Gene:COL18A1
Gene Synonym:
Exon (35)2954..3098
Gene:COL18A1
Gene Synonym:
Exon (36)3099..3172
Gene:COL18A1
Gene Synonym:
Exon (37)3173..3301
Gene:COL18A1
Gene Synonym:
Exon (38)3302..3334
Gene:COL18A1
Gene Synonym:
Exon (39)3335..3580
Gene:COL18A1
Gene Synonym:
Exon (40)3581..3778
Gene:COL18A1
Gene Synonym:
Exon (41)3779..3894
Gene:COL18A1
Gene Synonym:
Exon (42)3895..5427
Gene:COL18A1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_130445
Organism Homo sapiens (human)
Definition Homo sapiens collagen, type XVIII, alpha 1 (COL18A1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_130445

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGCGCCGA GGTGCCCCTG GCCATGGCCG CGGCGGCGGC GCCTCCTGGA CGTGCTCGCG 
CCCCTGGTCC TGCTGCTCGG GGTCCGCGCG GCCTCCGCGG AGCCAGAGCG CATCAGCGAG
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19882
Clone ID Related Accession (Same CDS sequence) NM_030582
Accession Version NM_030582.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-1(XVIII) chain isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX322561.1, BC063833.1 and AF018082.1. This sequence is a reference standard in the RefSeqGene project. On Jul 20, 2006 this sequence version replaced gi:18765744. Transcript Variant: This variant (1, also known as NCI-493) uses an alternate splice donor in the 5' terminal exon compared to variant 3. The encoded isoform (1) is shorter than isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGAGCG CATCAGCGAG
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_085059.2
CDS22..4572
Misc Feature(1)85..606(+)
Misc Feature(2)682..1248(+)
Misc Feature(3)1771..1884(+)
Misc Feature(4)2485..2637(+)
Misc Feature(5)2737..2859(+)
Misc Feature(6)3625..4569(+)
Misc Feature(7)4039..4551(+)
Misc Feature(8)4132..4437(+)
Exon (1)1..667
Gene:COL18A1
Gene Synonym:
Exon (2)668..1212
Gene:COL18A1
Gene Synonym:
Exon (3)1213..1299
Gene:COL18A1
Gene Synonym:
Exon (4)1300..1359
Gene:COL18A1
Gene Synonym:
Exon (5)1360..1489
Gene:COL18A1
Gene Synonym:
Exon (6)1490..1566
Gene:COL18A1
Gene Synonym:
Exon (7)1567..1782
Gene:COL18A1
Gene Synonym:
Exon (8)1783..1809
Gene:COL18A1
Gene Synonym:
Exon (9)1810..1872
Gene:COL18A1
Gene Synonym:
Exon (10)1873..1959
Gene:COL18A1
Gene Synonym:
Exon (11)1960..2013
Gene:COL18A1
Gene Synonym:
Exon (12)2014..2172
Gene:COL18A1
Gene Synonym:
Exon (13)2173..2235
Gene:COL18A1
Gene Synonym:
Exon (14)2236..2262
Gene:COL18A1
Gene Synonym:
Exon (15)2263..2394
Gene:COL18A1
Gene Synonym:
Exon (16)2395..2457
Gene:COL18A1
Gene Synonym:
Exon (17)2458..2484
Gene:COL18A1
Gene Synonym:
Exon (18)2485..2520
Gene:COL18A1
Gene Synonym:
Exon (19)2521..2592
Gene:COL18A1
Gene Synonym:
Exon (20)2593..2628
Gene:COL18A1
Gene Synonym:
Exon (21)2629..2718
Gene:COL18A1
Gene Synonym:
Exon (22)2719..2748
Gene:COL18A1
Gene Synonym:
Exon (23)2749..2775
Gene:COL18A1
Gene Synonym:
Exon (24)2776..2838
Gene:COL18A1
Gene Synonym:
Exon (25)2839..2913
Gene:COL18A1
Gene Synonym:
Exon (26)2914..2940
Gene:COL18A1
Gene Synonym:
Exon (27)2941..2994
Gene:COL18A1
Gene Synonym:
Exon (28)2995..3069
Gene:COL18A1
Gene Synonym:
Exon (29)3070..3138
Gene:COL18A1
Gene Synonym:
Exon (30)3139..3181
Gene:COL18A1
Gene Synonym:
Exon (31)3182..3244
Gene:COL18A1
Gene Synonym:
Exon (32)3245..3288
Gene:COL18A1
Gene Synonym:
Exon (33)3289..3420
Gene:COL18A1
Gene Synonym:
Exon (34)3421..3565
Gene:COL18A1
Gene Synonym:
Exon (35)3566..3639
Gene:COL18A1
Gene Synonym:
Exon (36)3640..3768
Gene:COL18A1
Gene Synonym:
Exon (37)3769..3801
Gene:COL18A1
Gene Synonym:
Exon (38)3802..4047
Gene:COL18A1
Gene Synonym:
Exon (39)4048..4245
Gene:COL18A1
Gene Synonym:
Exon (40)4246..4361
Gene:COL18A1
Gene Synonym:
Exon (41)4362..5894
Gene:COL18A1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_030582
Organism Homo sapiens (human)
Definition Homo sapiens collagen, type XVIII, alpha 1 (COL18A1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_030582

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGAGCG CATCAGCGAG
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu72836
Clone ID Related Accession (Same CDS sequence) NM_130444
Accession Version NM_130444.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product collagen alpha-1(XVIII) chain isoform 3 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX322561.1, BC063833.1 and AF018082.1. On Sep 25, 2008 this sequence version replaced gi:18765745. Transcript Variant: This variant (3, also known as NCI-728) represents the longest transcript and encodes the longest isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 14614989 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGGTAG AGCTTCTCTC
TCCTCCTTGC TGGGCGGGGC CCCTCCCTGG GGAAGCCTGC AGGACCCAGA CAGCCAAGGA
CTCTCGCCCG CCGCAGCCGC TCCCAGCCAG CAGCTCCAAC GCCCTGACGT CCGCCTGCGC
ACGCCACTTC TGCACCCCCT GGTGATGGGC TCCCTGGGCA AGCACGCGGC CCCCTCCGCC
TTCTCCTCTG GGCTCCCGGG CGCACTGTCT CAGGTCGCAG TCACCACTTT AACCAGGGAC
AGCGGTGCTT GGGTCTCCCA CGTGGCTAAC TCTGTGGGGC CGGGTCTTGC TAATAACTCT
GCCCTGCTCG GGGCTGACCC CGAGGCCCCC GCCGGTCGCT GCCTGCCCCT GCCACCCTCC
CTGCCAGTCT GCGGCCACCT GGGCATCTCA CGCTTCTGGC TGCCCAACCA CCTCCACCAC
GAGAGCGGCG AGCAGGTGCG GGCCGGGGCA CGGGCGTGGG GGGGCCTGCT GCAGACGCAC
TGCCACCCCT TCCTCGCCTG GTTCTTCTGC CTGCTGCTGG TCCCCCCATG CGGCAGCGTC
CCGCCGCCCG CCCCGCCACC CTGCTGCCAG TTCTGCGAGG CCCTGCAGGA TGCGTGTTGG
AGCCGCCTGG GCGGGGGCCG GCTGCCCGTC GCCTGTGCCT CGCTCCCGAC CCAGGAGGAT
GGGTACTGTG TGCTCATTGG GCCGGCTGCA GAGCGCATCA GCGAGGAGGT GGGGCTGCTG
CAGCTCCTTG GGGACCCCCC GCCCCAGCAG GTCACCCAGA CGGATGACCC CGACGTCGGG
CTGGCCTACG TCTTTGGGCC AGATGCCAAC AGTGGCCAAG TGGCCCGGTA CCACTTCCCC
AGCCTCTTCT TCCGTGACTT CTCACTGCTG TTCCACATCC GGCCAGCCAC AGAGGGCCCA
GGGGTGCTGT TCGCCATCAC GGACTCGGCG CAGGCCATGG TCTTGCTGGG CGTGAAGCTC
TCTGGGGTGC AGGACGGGCA CCAGGACATC TCCCTGCTCT ACACAGAACC AGGTGCAGGC
CAGACCCACA CAGCCGCCAG CTTCCGGCTC CCCGCCTTCG TCGGCCAGTG GACACACTTA
GCCCTCAGTG TGGCAGGTGG CTTTGTGGCC CTCTACGTGG ACTGTGAGGA GTTCCAGAGA
ATGCCGCTTG CTCGGTCCTC ACGGGGCCTG GAGCTGGAGC CTGGCGCCGG GCTCTTCGTG
GCTCAGGCGG GGGGAGCGGA CCCTGACAAG TTCCAGGGGG TGATCGCTGA GCTGAAGGTG
CGCAGGGACC CCCAGGTGAG CCCCATGCAC TGCCTGGACG AGGAAGGCGA TGACTCAGAT
GGGGCATCCG GAGACTCTGG CAGCGGGCTC GGGGACGCCC GGGAGCTTCT CAGGGAGGAG
ACGGGCGCGG CCCTAAAACC CAGGCTCCCC GCGCCACCCC CCGTCACCAC GCCACCCTTG
GCTGGAGGCA GCAGCACGGA AGATTCCAGA AGTGAAGAAG TCGAGGAGCA GACCACGGTG
GCTTCGTTAG GAGCTCAGAC ACTTCCTGGC TCAGATTCTG TCTCCACGTG GGACGGGAGT
GTCCGGACCC CTGGGGGCCG CGTGAAAGAG GGCGGCCTGA AGGGGCAGAA AGGGGAGCCA
GGTGTTCCGG GCCCACCTGG CCGGGCAGGC CCCCCAGGAT CCCCATGCCT ACCTGGTCCC
CCGGGTCTCC CGTGCCCAGT GAGTCCCCTG GGTCCTGCAG GCCCAGCGTT GCAAACTGTC
CCCGGACCAC AAGGACCCCC AGGGCCTCCG GGGAGGGACG GCACCCCTGG AAGGGACGGC
GAGCCGGGCG ACCCCGGTGA AGACGGAAAG CCGGGCGACA CCGGGCCACA AGGCTTCCCC
GGGACTCCAG GGGACGTAGG TCCCAAGGGC GACAAGGGAG ACCCTGGGGT TGGAGAGAGA
GGGCCCCCAG GACCCCAAGG GCCTCCAGGG CCCCCAGGAC CCTCCTTCAG ACACGACAAG
CTGACCTTCA TTGACATGGA GGGATCTGGC TTCGGGGGCG ATCTGGAGGC CCTGCGGGGT
CCTCGAGGCT TCCCTGGACC TCCCGGACCC CCCGGTGTCC CAGGCCTGCC CGGCGAGCCA
GGCCGCTTTG GGGTGAACAG CTCCGACGTC CCAGGACCCG CCGGCCTTCC TGGTGTGCCT
GGGCGCGAGG GTCCCCCCGG GTTTCCTGGC CTCCCGGGAC CCCCAGGCCC TCCGGGAAGA
GAGGGGCCCC CAGGAAGGAC TGGGCAGAAA GGCAGCCTGG GTGAAGCAGG CGCCCCAGGA
CATAAGGGGA GCAAGGGAGC CCCCGGTCCT GCTGGTGCTC GTGGGGAGAG CGGCCTGGCA
GGAGCCCCCG GACCTGCTGG ACCACCAGGC CCCCCTGGGC CCCCTGGGCC CCCAGGACCA
GGACTCCCCG CTGGATTTGA TGACATGGAA GGCTCCGGGG GGCCCTTCTG GTCAACAGCC
CGAAGCGCTG ATGGGCCACA GGGACCTCCC GGCCTGCCGG GACTTAAGGG GGATCCTGGC
GTGCCTGGGC TGCCGGGGGC GAAGGGAGAA GTTGGAGCAG ATGGAGTCCC CGGGTTCCCC
GGCCTCCCTG GCAGAGAGGG CATTGCTGGG CCCCAGGGGC CAAAGGGAGA CAGAGGCAGC
CGGGGAGAAA AGGGAGATCC AGGGAAGGAC GGAGTCGGGC AGCCGGGCCT CCCTGGCCCC
CCCGGACCCC CGGGACCTGT GGTCTACGTG TCGGAGCAGG ACGGATCCGT CCTGAGCGTG
CCGGGACCTG AGGGCCGGCC GGGTTTCGCA GGCTTTCCCG GACCTGCAGG ACCCAAGGGC
AACCTGGGCT CTAAGGGCGA ACGAGGCTCC CCGGGACCCA AGGGTGAGAA GGGTGAACCG
GGCAGCATCT TCAGCCCCGA CGGCGGTGCC CTGGGCCCTG CCCAGAAAGG AGCCAAGGGA
GAGCCGGGCT TCCGAGGACC CCCGGGTCCA TACGGACGGC CGGGGTACAA GGGAGAGATT
GGCTTTCCTG GACGGCCGGG TCGCCCCGGG ATGAACGGAT TGAAAGGAGA GAAAGGGGAG
CCGGGAGATG CCAGCCTTGG ATTTGGCATG AGGGGAATGC CCGGCCCCCC AGGACCTCCA
GGGCCCCCAG GCCCTCCAGG GACTCCTGTT TACGACAGCA ATGTGTTTGC TGAGTCCAGC
CGCCCCGGGC CTCCAGGATT GCCAGGGAAT CAGGGCCCTC CAGGACCCAA GGGCGCCAAA
GGAGAAGTGG GCCCCCCCGG ACCACCAGGG CAGTTTCCGT TTGACTTTCT TCAGTTGGAG
GCTGAAATGA AGGGGGAGAA GGGAGACCGA GGTGATGCAG GACAGAAAGG CGAAAGGGGG
GAGCCCGGGG GCGGCGGTTT CTTCGGCTCC AGCCTGCCCG GCCCCCCCGG CCCCCCAGGC
CCACGTGGCT ACCCTGGGAT TCCAGGTCCC AAGGGAGAGA GCATCCGGGG CCAGCCCGGC
CCACCTGGAC CTCAGGGACC CCCCGGCATC GGCTACGAGG GGCGCCAGGG CCCTCCCGGC
CCCCCAGGCC CCCCAGGGCC CCCTTCATTT CCTGGCCCTC ACAGGCAGAC TATCAGCGTT
CCCGGCCCTC CGGGCCCCCC TGGGCCCCCT GGGCCCCCTG GAACCATGGG CGCCTCCTCA
GGGGTGAGGC TCTGGGCTAC ACGCCAGGCC ATGCTGGGCC AGGTGCACGA GGTTCCCGAG
GGCTGGCTCA TCTTCGTGGC CGAGCAGGAG GAGCTCTACG TCCGCGTGCA GAACGGGTTC
CGGAAGGTCC AGCTGGAGGC CCGGACACCA CTCCCACGAG GGACGGACAA TGAAGTGGCC
GCCTTGCAGC CCCCCGTGGT GCAGCTGCAC GACAGCAACC CCTACCCGCG GCGGGAGCAC
CCCCACCCCA CCGCGCGGCC CTGGCGGGCA GATGACATCC TGGCCAGCCC CCCTCGCCTG
CCCGAGCCCC AGCCCTACCC CGGAGCCCCG CACCACAGCT CCTACGTGCA CCTGCGGCCG
GCGCGACCCA CAAGCCCACC CGCCCACAGC CACCGCGACT TCCAGCCGGT GCTCCACCTG
GTTGCGCTCA ACAGCCCCCT GTCAGGCGGC ATGCGGGGCA TCCGCGGGGC CGACTTCCAG
TGCTTCCAGC AGGCGCGGGC CGTGGGGCTG GCGGGCACCT TCCGCGCCTT CCTGTCCTCG
CGCCTGCAGG ACCTGTACAG CATCGTGCGC CGTGCCGACC GCGCAGCCGT GCCCATCGTC
AACCTCAAGG ACGAGCTGCT GTTTCCCAGC TGGGAGGCTC TGTTCTCAGG CTCTGAGGGT
CCGCTGAAGC CCGGGGCACG CATCTTCTCC TTTGACGGCA AGGACGTCCT GAGGCACCCC
ACCTGGCCCC AGAAGAGCGT GTGGCATGGC TCGGACCCCA ACGGGCGCAG GCTGACCGAG
AGCTACTGTG AGACGTGGCG GACGGAGGCT CCCTCGGCCA CGGGCCAGGC CTCCTCGCTG
CTGGGGGGCA GGCTCCTGGG GCAGAGTGCC GCGAGCTGCC ATCACGCCTA CATCGTGCTC
TGCATTGAGA ACAGCTTCAT GACTGCCTCC AAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_569711.2
CDS22..5277
Misc Feature(1)85..606(+)
Misc Feature(2)1009..1377(+)
Misc Feature(3)1048..1074(+)
Misc Feature(4)1387..1953(+)
Misc Feature(5)2476..2589(+)
Misc Feature(6)3190..3342(+)
Misc Feature(7)3442..3564(+)
Misc Feature(8)4330..5274(+)
Misc Feature(9)4744..5256(+)
Misc Feature(10)4837..5142(+)
Exon (1)1..1372
Gene:COL18A1
Gene Synonym:
Exon (2)1373..1917
Gene:COL18A1
Gene Synonym:
Exon (3)1918..2004
Gene:COL18A1
Gene Synonym:
Exon (4)2005..2064
Gene:COL18A1
Gene Synonym:
Exon (5)2065..2194
Gene:COL18A1
Gene Synonym:
Exon (6)2195..2271
Gene:COL18A1
Gene Synonym:
Exon (7)2272..2487
Gene:COL18A1
Gene Synonym:
Exon (8)2488..2514
Gene:COL18A1
Gene Synonym:
Exon (9)2515..2577
Gene:COL18A1
Gene Synonym:
Exon (10)2578..2664
Gene:COL18A1
Gene Synonym:
Exon (11)2665..2718
Gene:COL18A1
Gene Synonym:
Exon (12)2719..2877
Gene:COL18A1
Gene Synonym:
Exon (13)2878..2940
Gene:COL18A1
Gene Synonym:
Exon (14)2941..2967
Gene:COL18A1
Gene Synonym:
Exon (15)2968..3099
Gene:COL18A1
Gene Synonym:
Exon (16)3100..3162
Gene:COL18A1
Gene Synonym:
Exon (17)3163..3189
Gene:COL18A1
Gene Synonym:
Exon (18)3190..3225
Gene:COL18A1
Gene Synonym:
Exon (19)3226..3297
Gene:COL18A1
Gene Synonym:
Exon (20)3298..3333
Gene:COL18A1
Gene Synonym:
Exon (21)3334..3423
Gene:COL18A1
Gene Synonym:
Exon (22)3424..3453
Gene:COL18A1
Gene Synonym:
Exon (23)3454..3480
Gene:COL18A1
Gene Synonym:
Exon (24)3481..3543
Gene:COL18A1
Gene Synonym:
Exon (25)3544..3618
Gene:COL18A1
Gene Synonym:
Exon (26)3619..3645
Gene:COL18A1
Gene Synonym:
Exon (27)3646..3699
Gene:COL18A1
Gene Synonym:
Exon (28)3700..3774
Gene:COL18A1
Gene Synonym:
Exon (29)3775..3843
Gene:COL18A1
Gene Synonym:
Exon (30)3844..3886
Gene:COL18A1
Gene Synonym:
Exon (31)3887..3949
Gene:COL18A1
Gene Synonym:
Exon (32)3950..3993
Gene:COL18A1
Gene Synonym:
Exon (33)3994..4125
Gene:COL18A1
Gene Synonym:
Exon (34)4126..4270
Gene:COL18A1
Gene Synonym:
Exon (35)4271..4344
Gene:COL18A1
Gene Synonym:
Exon (36)4345..4473
Gene:COL18A1
Gene Synonym:
Exon (37)4474..4506
Gene:COL18A1
Gene Synonym:
Exon (38)4507..4752
Gene:COL18A1
Gene Synonym:
Exon (39)4753..4950
Gene:COL18A1
Gene Synonym:
Exon (40)4951..5066
Gene:COL18A1
Gene Synonym:
Exon (41)5067..6599
Gene:COL18A1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_130444
Organism Homo sapiens (human)
Definition Homo sapiens collagen, type XVIII, alpha 1 (COL18A1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_130444

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGGTAG AGCTTCTCTC
TCCTCCTTGC TGGGCGGGGC CCCTCCCTGG GGAAGCCTGC AGGACCCAGA CAGCCAAGGA
CTCTCGCCCG CCGCAGCCGC TCCCAGCCAG CAGCTCCAAC GCCCTGACGT CCGCCTGCGC
ACGCCACTTC TGCACCCCCT GGTGATGGGC TCCCTGGGCA AGCACGCGGC CCCCTCCGCC
TTCTCCTCTG GGCTCCCGGG CGCACTGTCT CAGGTCGCAG TCACCACTTT AACCAGGGAC
AGCGGTGCTT GGGTCTCCCA CGTGGCTAAC TCTGTGGGGC CGGGTCTTGC TAATAACTCT
GCCCTGCTCG GGGCTGACCC CGAGGCCCCC GCCGGTCGCT GCCTGCCCCT GCCACCCTCC
CTGCCAGTCT GCGGCCACCT GGGCATCTCA CGCTTCTGGC TGCCCAACCA CCTCCACCAC
GAGAGCGGCG AGCAGGTGCG GGCCGGGGCA CGGGCGTGGG GGGGCCTGCT GCAGACGCAC
TGCCACCCCT TCCTCGCCTG GTTCTTCTGC CTGCTGCTGG TCCCCCCATG CGGCAGCGTC
CCGCCGCCCG CCCCGCCACC CTGCTGCCAG TTCTGCGAGG CCCTGCAGGA TGCGTGTTGG
AGCCGCCTGG GCGGGGGCCG GCTGCCCGTC GCCTGTGCCT CGCTCCCGAC CCAGGAGGAT
GGGTACTGTG TGCTCATTGG GCCGGCTGCA GAGCGCATCA GCGAGGAGGT GGGGCTGCTG
CAGCTCCTTG GGGACCCCCC GCCCCAGCAG GTCACCCAGA CGGATGACCC CGACGTCGGG
CTGGCCTACG TCTTTGGGCC AGATGCCAAC AGTGGCCAAG TGGCCCGGTA CCACTTCCCC
AGCCTCTTCT TCCGTGACTT CTCACTGCTG TTCCACATCC GGCCAGCCAC AGAGGGCCCA
GGGGTGCTGT TCGCCATCAC GGACTCGGCG CAGGCCATGG TCTTGCTGGG CGTGAAGCTC
TCTGGGGTGC AGGACGGGCA CCAGGACATC TCCCTGCTCT ACACAGAACC AGGTGCAGGC
CAGACCCACA CAGCCGCCAG CTTCCGGCTC CCCGCCTTCG TCGGCCAGTG GACACACTTA
GCCCTCAGTG TGGCAGGTGG CTTTGTGGCC CTCTACGTGG ACTGTGAGGA GTTCCAGAGA
ATGCCGCTTG CTCGGTCCTC ACGGGGCCTG GAGCTGGAGC CTGGCGCCGG GCTCTTCGTG
GCTCAGGCGG GGGGAGCGGA CCCTGACAAG TTCCAGGGGG TGATCGCTGA GCTGAAGGTG
CGCAGGGACC CCCAGGTGAG CCCCATGCAC TGCCTGGACG AGGAAGGCGA TGACTCAGAT
GGGGCATCCG GAGACTCTGG CAGCGGGCTC GGGGACGCCC GGGAGCTTCT CAGGGAGGAG
ACGGGCGCGG CCCTAAAACC CAGGCTCCCC GCGCCACCCC CCGTCACCAC GCCACCCTTG
GCTGGAGGCA GCAGCACGGA AGATTCCAGA AGTGAAGAAG TCGAGGAGCA GACCACGGTG
GCTTCGTTAG GAGCTCAGAC ACTTCCTGGC TCAGATTCTG TCTCCACGTG GGACGGGAGT
GTCCGGACCC CTGGGGGCCG CGTGAAAGAG GGCGGCCTGA AGGGGCAGAA AGGGGAGCCA
GGTGTTCCGG GCCCACCTGG CCGGGCAGGC CCCCCAGGAT CCCCATGCCT ACCTGGTCCC
CCGGGTCTCC CGTGCCCAGT GAGTCCCCTG GGTCCTGCAG GCCCAGCGTT GCAAACTGTC
CCCGGACCAC AAGGACCCCC AGGGCCTCCG GGGAGGGACG GCACCCCTGG AAGGGACGGC
GAGCCGGGCG ACCCCGGTGA AGACGGAAAG CCGGGCGACA CCGGGCCACA AGGCTTCCCC
GGGACTCCAG GGGACGTAGG TCCCAAGGGC GACAAGGGAG ACCCTGGGGT TGGAGAGAGA
GGGCCCCCAG GACCCCAAGG GCCTCCAGGG CCCCCAGGAC CCTCCTTCAG ACACGACAAG
CTGACCTTCA TTGACATGGA GGGATCTGGC TTCGGGGGCG ATCTGGAGGC CCTGCGGGGT
CCTCGAGGCT TCCCTGGACC TCCCGGACCC CCCGGTGTCC CAGGCCTGCC CGGCGAGCCA
GGCCGCTTTG GGGTGAACAG CTCCGACGTC CCAGGACCCG CCGGCCTTCC TGGTGTGCCT
GGGCGCGAGG GTCCCCCCGG GTTTCCTGGC CTCCCGGGAC CCCCAGGCCC TCCGGGAAGA
GAGGGGCCCC CAGGAAGGAC TGGGCAGAAA GGCAGCCTGG GTGAAGCAGG CGCCCCAGGA
CATAAGGGGA GCAAGGGAGC CCCCGGTCCT GCTGGTGCTC GTGGGGAGAG CGGCCTGGCA
GGAGCCCCCG GACCTGCTGG ACCACCAGGC CCCCCTGGGC CCCCTGGGCC CCCAGGACCA
GGACTCCCCG CTGGATTTGA TGACATGGAA GGCTCCGGGG GGCCCTTCTG GTCAACAGCC
CGAAGCGCTG ATGGGCCACA GGGACCTCCC GGCCTGCCGG GACTTAAGGG GGATCCTGGC
GTGCCTGGGC TGCCGGGGGC GAAGGGAGAA GTTGGAGCAG ATGGAGTCCC CGGGTTCCCC
GGCCTCCCTG GCAGAGAGGG CATTGCTGGG CCCCAGGGGC CAAAGGGAGA CAGAGGCAGC
CGGGGAGAAA AGGGAGATCC AGGGAAGGAC GGAGTCGGGC AGCCGGGCCT CCCTGGCCCC
CCCGGACCCC CGGGACCTGT GGTCTACGTG TCGGAGCAGG ACGGATCCGT CCTGAGCGTG
CCGGGACCTG AGGGCCGGCC GGGTTTCGCA GGCTTTCCCG GACCTGCAGG ACCCAAGGGC
AACCTGGGCT CTAAGGGCGA ACGAGGCTCC CCGGGACCCA AGGGTGAGAA GGGTGAACCG
GGCAGCATCT TCAGCCCCGA CGGCGGTGCC CTGGGCCCTG CCCAGAAAGG AGCCAAGGGA
GAGCCGGGCT TCCGAGGACC CCCGGGTCCA TACGGACGGC CGGGGTACAA GGGAGAGATT
GGCTTTCCTG GACGGCCGGG TCGCCCCGGG ATGAACGGAT TGAAAGGAGA GAAAGGGGAG
CCGGGAGATG CCAGCCTTGG ATTTGGCATG AGGGGAATGC CCGGCCCCCC AGGACCTCCA
GGGCCCCCAG GCCCTCCAGG GACTCCTGTT TACGACAGCA ATGTGTTTGC TGAGTCCAGC
CGCCCCGGGC CTCCAGGATT GCCAGGGAAT CAGGGCCCTC CAGGACCCAA GGGCGCCAAA
GGAGAAGTGG GCCCCCCCGG ACCACCAGGG CAGTTTCCGT TTGACTTTCT TCAGTTGGAG
GCTGAAATGA AGGGGGAGAA GGGAGACCGA GGTGATGCAG GACAGAAAGG CGAAAGGGGG
GAGCCCGGGG GCGGCGGTTT CTTCGGCTCC AGCCTGCCCG GCCCCCCCGG CCCCCCAGGC
CCACGTGGCT ACCCTGGGAT TCCAGGTCCC AAGGGAGAGA GCATCCGGGG CCAGCCCGGC
CCACCTGGAC CTCAGGGACC CCCCGGCATC GGCTACGAGG GGCGCCAGGG CCCTCCCGGC
CCCCCAGGCC CCCCAGGGCC CCCTTCATTT CCTGGCCCTC ACAGGCAGAC TATCAGCGTT
CCCGGCCCTC CGGGCCCCCC TGGGCCCCCT GGGCCCCCTG GAACCATGGG CGCCTCCTCA
GGGGTGAGGC TCTGGGCTAC ACGCCAGGCC ATGCTGGGCC AGGTGCACGA GGTTCCCGAG
GGCTGGCTCA TCTTCGTGGC CGAGCAGGAG GAGCTCTACG TCCGCGTGCA GAACGGGTTC
CGGAAGGTCC AGCTGGAGGC CCGGACACCA CTCCCACGAG GGACGGACAA TGAAGTGGCC
GCCTTGCAGC CCCCCGTGGT GCAGCTGCAC GACAGCAACC CCTACCCGCG GCGGGAGCAC
CCCCACCCCA CCGCGCGGCC CTGGCGGGCA GATGACATCC TGGCCAGCCC CCCTCGCCTG
CCCGAGCCCC AGCCCTACCC CGGAGCCCCG CACCACAGCT CCTACGTGCA CCTGCGGCCG
GCGCGACCCA CAAGCCCACC CGCCCACAGC CACCGCGACT TCCAGCCGGT GCTCCACCTG
GTTGCGCTCA ACAGCCCCCT GTCAGGCGGC ATGCGGGGCA TCCGCGGGGC CGACTTCCAG
TGCTTCCAGC AGGCGCGGGC CGTGGGGCTG GCGGGCACCT TCCGCGCCTT CCTGTCCTCG
CGCCTGCAGG ACCTGTACAG CATCGTGCGC CGTGCCGACC GCGCAGCCGT GCCCATCGTC
AACCTCAAGG ACGAGCTGCT GTTTCCCAGC TGGGAGGCTC TGTTCTCAGG CTCTGAGGGT
CCGCTGAAGC CCGGGGCACG CATCTTCTCC TTTGACGGCA AGGACGTCCT GAGGCACCCC
ACCTGGCCCC AGAAGAGCGT GTGGCATGGC TCGGACCCCA ACGGGCGCAG GCTGACCGAG
AGCTACTGTG AGACGTGGCG GACGGAGGCT CCCTCGGCCA CGGGCCAGGC CTCCTCGCTG
CTGGGGGGCA GGCTCCTGGG GCAGAGTGCC GCGAGCTGCC ATCACGCCTA CATCGTGCTC
TGCATTGAGA ACAGCTTCAT GACTGCCTCC AAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Brain malformations associated with knobloch syndrome-review of literature, expanding clinical spectrum, and identification of novel mutations
Pediatr. Neurol. 51 (6), 806-813 (2014)
Caglayan AO, Baranoski JF, Aktar F, Han W, Tuysuz B, Guzel A, Guclu B, Kaymakcalan H, Aktekin B, Akgumus GT, Murray PB, Erson-Omay EZ, Caglar C, Bakircioglu M, Sakalar YB, Guzel E, Demir N, Tuncer O, Senturk S, Ekici B, Minja FJ, Sestan N, Yasuno K, Bilguvar K, Caksen H and Gunel M.


book

Associations of Endothelial System Genes With Blood Pressure Changes and Hypertension Incidence: The GenSalt Study
Am. J. Hypertens. (2014) In press
Liu F, He J, Gu D, Rao DC, Huang J, Hixson JE, Jaquish CE, Chen J, Li C, Yang X, Li J, Rice TK, Shimmin LC and Kelly TN.


book

Homozygosity Mapping and Whole Exome Sequencing Reveal a Novel Homozygous COL18A1 Mutation Causing Knobloch Syndrome
PLoS ONE 9 (11), E112747 (2014)
Haghighi A, Tiwari A, Piri N, Nurnberg G, Saleh-Gohari N, Haghighi A, Neidhardt J, Nurnberg P and Berger W.


book

The multiple functions of collagen XVIII in development and disease
Matrix Biol. 30 (2), 83-92 (2011)
Seppinen L and Pihlajaniemi T.


book

Characterization of the human type XVIII collagen gene and proteolytic processing and tissue location of the variant containing a frizzled motif
Matrix Biol. 22 (5), 427-442 (2003)
Elamaa H, Snellman A, Rehn M, Autio-Harmainen H and Pihlajaniemi T.


book

Secreted cathepsin L generates endostatin from collagen XVIII
EMBO J. 19 (6), 1187-1194 (2000)
Felbor U, Dreier L, Bryant RA, Ploegh HL, Olsen BR and Mothes W.


book

Complete primary structure of two variant forms of human type XVIII collagen and tissue-specific differences in the expression of the corresponding transcripts
Matrix Biol. 16 (6), 319-328 (1998)
Saarela J, Ylikarppa R, Rehn M, Purmonen S and Pihlajaniemi T.


book

Isolation and characterization of the circulating form of human endostatin
FEBS Lett. 420 (2-3), 129-133 (1997)
Standker L, Schrader M, Kanse SM, Jurgens M, Forssmann WG and Preissner KT.


book

Isolation and sequencing of cDNAs for proteins with multiple domains of Gly-Xaa-Yaa repeats identify a distinct family of collagenous proteins
Proc. Natl. Acad. Sci. U.S.A. 91 (10), 4229-4233 (1994)
Oh SP, Kamagata Y, Muragaki Y, Timmons S, Ooshima A and Olsen BR.


book

Cloning of cDNA and genomic DNA encoding human type XVIII collagen and localization of the alpha 1(XVIII) collagen gene to mouse chromosome 10 and human chromosome 21
Genomics 19 (3), 494-499 (1994)
Oh SP, Warman ML, Seldin MF, Cheng SD, Knoll JH, Timmons S and Olsen BR.


 
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