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MADCAM1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MADCAM1
Entrez Gene ID 8174
Full Name mucosal vascular addressin cell adhesion molecule 1
Synonyms MACAM1
General protein information
Preferred Names
mucosal addressin cell adhesion molecule 1
Names
mucosal addressin cell adhesion molecule 1
MAdCAM-1
hMAdCAM-1
mucosal addressin cell adhesion molecule-1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19p13.3

Summary The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

102670


mRNA and Protein(s)

mRNA Protein Name
NM_130762 NP_570118 mucosal addressin cell adhesion molecule 1 isoform b precursor
NM_130760 NP_570116 mucosal addressin cell adhesion molecule 1 isoform a precursor



GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MADCAM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MADCAM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu13360 NM_130762 Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $216.30
$309.00
OHu13301 NM_130760 Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu13360
Clone ID Related Accession (Same CDS sequence) NM_130762
Accession Version NM_130762.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 888bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucosal addressin cell adhesion molecule 1 isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U82483.1 and AY732484.1. On Jun 23, 2006 this sequence version replaced gi:18780284. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform b) that lacks an internal segment, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC137506.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2152568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGATTTCG GACTGGCCCT CCTGCTGGCG GGGCTTCTGG GGCTCCTCCT CGGCCAGTCC 
CTCCAGGTGA AGCCCCTGCA GGTGGAGCCC CCGGAGCCGG TGGTGGCCGT GGCCTTGGGC
GCCTCGCGCC AGCTCACCTG CCGCCTGGCC TGCGCGGACC GCGGGGCCTC GGTGCAGTGG
CGGGGCCTGG ACACCAGCCT GGGCGCGGTG CAGTCGGACA CGGGCCGCAG CGTCCTCACC
GTGCGCAACG CCTCGCTGTC GGCGGCCGGG ACCCGCGTGT GCGTGGGCTC CTGCGGGGGC
CGCACCTTCC AGCACACCGT GCAGCTCCTT GTGTACGCCT TCCCGGACCA GCTGACCGTC
TCCCCAGCAG CCCTGGTGCC TGGTGACCCG GAGGTGGCCT GTACGGCCCA CAAAGTCACG
CCCGTGGACC CCAACGCGCT CTCCTTCTCC CTGCTCGTCG GGGGCCAGGA ACTGGAGGGG
GCGCAAGCCC TGGGCCCGGA GGTGCAGGAG GAGGAGGAGG AGCCCCAGGG GGACGAGGAC
GTGCTGTTCA GGGTGACAGA GCGCTGGCGG CTGCCGCCCC TGGGGACCCC TGTCCCGCCC
GCCCTCTACT GCCAGGCCAC GATGAGGCTG CCTGGCTTGG AGCTCAGCCA CCGCCAGGCC
ATCCCCGCGT CCAAACCTGC GGGTGACCAG CTGCCCGCGG CTCTGTGGAC CAGCAGTGCG
GTGCTGGGAC TGCTGCTCCT GGCCTTGCCC ACCTATCACC TCTGGAAACG CTGCCGGCAC
CTGGCTGAGG ACGACACCCA CCCACCAGCT TCTCTGAGGC TTCTGCCCCA GGTGTCGGCC
TGGGCTGGGT TAAGGGGGAC CGGCCAGGTC GGGATCAGCC CCTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_570118.1
CDS11..898
Misc Feature(1)92..337(+)
Misc Feature(2)107..343(+)
Misc Feature(3)347..676(+)
Exon (1)1..62
Gene:MADCAM1
Gene Synonym:
Exon (2)63..347
Gene:MADCAM1
Gene Synonym:
Exon (3)348..677
Gene:MADCAM1
Gene Synonym:
Exon (4)678..1276
Gene:MADCAM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_130762
Organism Homo sapiens (human)
Definition Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_130762

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGATTTCG GACTGGCCCT CCTGCTGGCG GGGCTTCTGG GGCTCCTCCT CGGCCAGTCC 
CTCCAGGTGA AGCCCCTGCA GGTGGAGCCC CCGGAGCCGG TGGTGGCCGT GGCCTTGGGC
GCCTCGCGCC AGCTCACCTG CCGCCTGGCC TGCGCGGACC GCGGGGCCTC GGTGCAGTGG
CGGGGCCTGG ACACCAGCCT GGGCGCGGTG CAGTCGGACA CGGGCCGCAG CGTCCTCACC
GTGCGCAACG CCTCGCTGTC GGCGGCCGGG ACCCGCGTGT GCGTGGGCTC CTGCGGGGGC
CGCACCTTCC AGCACACCGT GCAGCTCCTT GTGTACGCCT TCCCGGACCA GCTGACCGTC
TCCCCAGCAG CCCTGGTGCC TGGTGACCCG GAGGTGGCCT GTACGGCCCA CAAAGTCACG
CCCGTGGACC CCAACGCGCT CTCCTTCTCC CTGCTCGTCG GGGGCCAGGA ACTGGAGGGG
GCGCAAGCCC TGGGCCCGGA GGTGCAGGAG GAGGAGGAGG AGCCCCAGGG GGACGAGGAC
GTGCTGTTCA GGGTGACAGA GCGCTGGCGG CTGCCGCCCC TGGGGACCCC TGTCCCGCCC
GCCCTCTACT GCCAGGCCAC GATGAGGCTG CCTGGCTTGG AGCTCAGCCA CCGCCAGGCC
ATCCCCGCGT CCAAACCTGC GGGTGACCAG CTGCCCGCGG CTCTGTGGAC CAGCAGTGCG
GTGCTGGGAC TGCTGCTCCT GGCCTTGCCC ACCTATCACC TCTGGAAACG CTGCCGGCAC
CTGGCTGAGG ACGACACCCA CCCACCAGCT TCTCTGAGGC TTCTGCCCCA GGTGTCGGCC
TGGGCTGGGT TAAGGGGGAC CGGCCAGGTC GGGATCAGCC CCTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13301
Clone ID Related Accession (Same CDS sequence) NM_130760
Accession Version NM_130760.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucosal addressin cell adhesion molecule 1 isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U82483.1, U43628.1 and DB323303.1. On or before Jun 23, 2006 this sequence version replaced gi:18780280, gi:18780276. Transcript Variant: This variant (1) is the longer, predominant transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U82483.1, AY819760.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTCG GACTGGCCCT CCTGCTGGCG GGGCTTCTGG GGCTCCTCCT CGGCCAGTCC 
CTCCAGGTGA AGCCCCTGCA GGTGGAGCCC CCGGAGCCGG TGGTGGCCGT GGCCTTGGGC
GCCTCGCGCC AGCTCACCTG CCGCCTGGCC TGCGCGGACC GCGGGGCCTC GGTGCAGTGG
CGGGGCCTGG ACACCAGCCT GGGCGCGGTG CAGTCGGACA CGGGCCGCAG CGTCCTCACC
GTGCGCAACG CCTCGCTGTC GGCGGCCGGG ACCCGCGTGT GCGTGGGCTC CTGCGGGGGC
CGCACCTTCC AGCACACCGT GCAGCTCCTT GTGTACGCCT TCCCGGACCA GCTGACCGTC
TCCCCAGCAG CCCTGGTGCC TGGTGACCCG GAGGTGGCCT GTACGGCCCA CAAAGTCACG
CCCGTGGACC CCAACGCGCT CTCCTTCTCC CTGCTCGTCG GGGGCCAGGA ACTGGAGGGG
GCGCAAGCCC TGGGCCCGGA GGTGCAGGAG GAGGAGGAGG AGCCCCAGGG GGACGAGGAC
GTGCTGTTCA GGGTGACAGA GCGCTGGCGG CTGCCGCCCC TGGGGACCCC TGTCCCGCCC
GCCCTCTACT GCCAGGCCAC GATGAGGCTG CCTGGCTTGG AGCTCAGCCA CCGCCAGGCC
ATCCCCGTCC TGCACAGCCC GACCTCCCCG GAGCCTCCCG ACACCACCTC CCCGGAGTCT
CCCGACACCA CCTCCCCGGA GTCTCCCGAC ACCACCTCCC AGGAGCCTCC CGACACCACC
TCCCCGGAGC CTCCCGACAA GACCTCCCCG GAGCCCGCCC CCCAGCAGGG CTCCACACAC
ACCCCCAGGA GCCCAGGCTC CACCAGGACT CGCCGCCCTG AGATCTCCCA GGCTGGGCCC
ACGCAGGGAG AAGTGATCCC AACAGGCTCG TCCAAACCTG CGGGTGACCA GCTGCCCGCG
GCTCTGTGGA CCAGCAGTGC GGTGCTGGGA CTGCTGCTCC TGGCCTTGCC CACCTATCAC
CTCTGGAAAC GCTGCCGGCA CCTGGCTGAG GACGACACCC ACCCACCAGC TTCTCTGAGG
CTTCTGCCCC AGGTGTCGGC CTGGGCTGGG TTAAGGGGGA CCGGCCAGGT CGGGATCAGC
CCCTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_570116.2
CDS11..1159
Misc Feature(1)86..337(+)
Misc Feature(2)107..343(+)
Misc Feature(3)347..682(+)
Misc Feature(4)704..823(+)
Misc Feature(5)962..1024(+)
Exon (1)1..62
Gene:MADCAM1
Gene Synonym:
Exon (2)63..347
Gene:MADCAM1
Gene Synonym:
Exon (3)348..677
Gene:MADCAM1
Gene Synonym:
Exon (4)678..938
Gene:MADCAM1
Gene Synonym:
Exon (5)939..1537
Gene:MADCAM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_130760
Organism Homo sapiens (human)
Definition Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_130760

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGATTTCG GACTGGCCCT CCTGCTGGCG GGGCTTCTGG GGCTCCTCCT CGGCCAGTCC 
CTCCAGGTGA AGCCCCTGCA GGTGGAGCCC CCGGAGCCGG TGGTGGCCGT GGCCTTGGGC
GCCTCGCGCC AGCTCACCTG CCGCCTGGCC TGCGCGGACC GCGGGGCCTC GGTGCAGTGG
CGGGGCCTGG ACACCAGCCT GGGCGCGGTG CAGTCGGACA CGGGCCGCAG CGTCCTCACC
GTGCGCAACG CCTCGCTGTC GGCGGCCGGG ACCCGCGTGT GCGTGGGCTC CTGCGGGGGC
CGCACCTTCC AGCACACCGT GCAGCTCCTT GTGTACGCCT TCCCGGACCA GCTGACCGTC
TCCCCAGCAG CCCTGGTGCC TGGTGACCCG GAGGTGGCCT GTACGGCCCA CAAAGTCACG
CCCGTGGACC CCAACGCGCT CTCCTTCTCC CTGCTCGTCG GGGGCCAGGA ACTGGAGGGG
GCGCAAGCCC TGGGCCCGGA GGTGCAGGAG GAGGAGGAGG AGCCCCAGGG GGACGAGGAC
GTGCTGTTCA GGGTGACAGA GCGCTGGCGG CTGCCGCCCC TGGGGACCCC TGTCCCGCCC
GCCCTCTACT GCCAGGCCAC GATGAGGCTG CCTGGCTTGG AGCTCAGCCA CCGCCAGGCC
ATCCCCGTCC TGCACAGCCC GACCTCCCCG GAGCCTCCCG ACACCACCTC CCCGGAGTCT
CCCGACACCA CCTCCCCGGA GTCTCCCGAC ACCACCTCCC AGGAGCCTCC CGACACCACC
TCCCCGGAGC CTCCCGACAA GACCTCCCCG GAGCCCGCCC CCCAGCAGGG CTCCACACAC
ACCCCCAGGA GCCCAGGCTC CACCAGGACT CGCCGCCCTG AGATCTCCCA GGCTGGGCCC
ACGCAGGGAG AAGTGATCCC AACAGGCTCG TCCAAACCTG CGGGTGACCA GCTGCCCGCG
GCTCTGTGGA CCAGCAGTGC GGTGCTGGGA CTGCTGCTCC TGGCCTTGCC CACCTATCAC
CTCTGGAAAC GCTGCCGGCA CCTGGCTGAG GACGACACCC ACCCACCAGC TTCTCTGAGG
CTTCTGCCCC AGGTGTCGGC CTGGGCTGGG TTAAGGGGGA CCGGCCAGGT CGGGATCAGC
CCCTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Mucosal addressin cell adhesion molecule (MAdCAM-1) expression is upregulated in the cirrhotic liver and immunolocalises to the peribiliary plexus and lymphoid aggregates
Dig. Dis. Sci. 58 (9), 2528-2541 (2013)
Ala A, Brown D, Khan K, Standish R, Odin JA, Fiel MI, Schiano TD, Hillan KJ, Rahman SA, Hodgson HJ and Dhillon AP.


book

Domain 1 of mucosal addressin cell adhesion molecule has an I1-set fold and a flexible integrin-binding loop
J. Biol. Chem. 288 (9), 6284-6294 (2013)
Yu Y, Zhu J, Huang PS, Wang JH, Pullen N and Springer TA.


book

The CC' and DE loops in Ig domains 1 and 2 of MAdCAM-1 play different roles in MAdCAM-1 binding to low- and high-affinity integrin alpha4beta7
J. Biol. Chem. 286 (14), 12086-12092 (2011)
Sun H, Wu Y, Qi J, Pan Y, Ge G and Chen J.


book

Possible impact of MADCAM1 gene single nucleotide polymorphisms to the outcome of allogeneic hematopoietic stem cell transplantation
Hum. Immunol. 70 (6), 457-460 (2009)
Ambruzova Z, Mrazek F, Raida L, Stahelova A, Faber E, Indrak K and Petrek M.


book

Increased expression of mucosal addressin cell adhesion molecule 1 in the duodenum of patients with active celiac disease is associated with depletion of integrin alpha4beta7-positive T cells in blood
Hum. Pathol. 40 (5), 699-704 (2009)
Di Sabatino A, Rovedatti L, Rosado MM, Carsetti R, Corazza GR and MacDonald TT.


book

Genomic organization, chromosomal mapping, and analysis of the 5' promoter region of the human MAdCAM-1 gene
Immunogenetics 46 (2), 111-119 (1997)
Leung E, Berg RW, Langley R, Greene J, Raymond LA, Augustus M, Ni J, Carter KC, Spurr N, Choo KH and Krissansen GW.


book

Cloning of the mucosal addressin MAdCAM-1 from human brain: identification of novel alternatively spliced transcripts
Immunol. Cell Biol. 74 (6), 490-496 (1996)
Leung E, Greene J, Ni J, Raymond LG, Lehnert K, Langley R and Krissansen GW.


book

Human mucosal addressin cell adhesion molecule-1 (MAdCAM-1) demonstrates structural and functional similarities to the alpha 4 beta 7-integrin binding domains of murine MAdCAM-1, but extreme divergence of mucin-like sequences
J. Immunol. 156 (8), 2851-2857 (1996)
Shyjan AM, Bertagnolli M, Kenney CJ and Briskin MJ.


book

MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1
Nature 363 (6428), 461-464 (1993)
Briskin MJ, McEvoy LM and Butcher EC.


book

Monoclonal antibodies to human lymphocyte homing receptors define a novel class of adhesion molecules on diverse cell types
J. Cell Biol. 109 (2), 927-937 (1989)
Picker LJ, Nakache M and Butcher EC.


 
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