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QTRT1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol QTRT1
Entrez Gene ID 81890
Full Name queuine tRNA-ribosyltransferase 1
Synonyms FP3235, TGT, TGUT
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19p13.3

Summary This gene encodes the catalytic subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine and tyrosine. A pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Feb 2012]. lac of sum
Disorder MIM:

609615


mRNA and Protein(s)

mRNA Protein Name
NM_031209 NP_112486 queuine tRNA-ribosyltransferase


Homo sapiens (human) QTRT1 NP_112486.1
Pan troglodytes (chimpanzee) QTRT1 XP_512381.1
Canis lupus familiaris (dog) QTRT1 XP_854015.1
Bos taurus (cattle) QTRT1 NP_001193621.1
Mus musculus (house mouse) Qtrt1 NP_068688.2
Rattus norvegicus (Norway rat) Qtrt1 NP_071586.2
Danio rerio (zebrafish) qtrt1 NP_957304.1
Drosophila melanogaster (fruit fly) Tgt NP_608585.1
Caenorhabditis elegans tgt-1 NP_502268.1
Xenopus (Silurana) tropicalis (western clawed frog) qtrt1 NP_989090.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following QTRT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the QTRT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu31882 NM_031209 Homo sapiens queuine tRNA-ribosyltransferase 1 (QTRT1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $237.30
$339.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu31882
Clone ID Related Accession (Same CDS sequence) NM_031209
Accession Version NM_031209.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1212bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product queuine tRNA-ribosyltransferase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CT003728.1, AF302783.1 and AF370379.1. On Jan 23, 2009 this sequence version replaced gi:13654275. ##Evidence-Data-START## Transcript exon combination :: AF302783.1, BC015350.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCGGGAG CAGCTACCCA GGCTTCCCTG GAGTCGGCCC CACGGATCAT GCGGCTGGTG 
GCCGAATGCA GCCGCTCCAG GGCCCGGGCA GGCGAGCTGT GGCTGCCGCA TGGGACAGTG
GCCACTCCTG TGTTCATGCC AGTGGGCACG CAGGCCACCA TGAAGGGCAT CACGACCGAA
CAGCTGGACG CTCTGGGTTG CCGCATCTGC CTGGGCAATA CCTACCATCT GGGTCTAAGG
CCGGGACCCG AGCTGATCCA GAAAGCCAAC GGTCTCCACG GCTTCATGAA TTGGCCTCAT
AATCTGCTAA CGGACAGCGG CGGTTTCCAG ATGGTGTCGC TGGTGTCTCT GTCCGAGGTG
ACGGAGGAGG GCGTCCGCTT CCGCTCCCCC TACGACGGCA ATGAGACCCT GCTGAGCCCG
GAGAAATCCG TGCAGATCCA GAATGCGCTG GGCTCGGACA TCATCATGCA GCTGGACGAC
GTGGTTAGCA GTACTGTGAC TGGGCCACGT GTGGAGGAGG CCATGTACAG GTCAATCCGC
TGGCTGGACC GGTGCATTGC AGCCCATCAG CGGCCGGACA AGCAGAACCT CTTCGCCATT
ATCCAGGGTG GGCTGGACGC AGATCTCCGG GCCACCTGCC TTGAAGAGAT GACCAAGCGA
GACGTGCCTG GCTTCGCCAT CGGGGGCCTG AGCGGGGGTG AGAGCAAGTC GCAGTTCTGG
CGGATGGTGG CGCTGAGCAC CTCTCGGCTG CCGAAGGACA AGCCCCGATA TCTGATGGGG
GTTGGCTATG CCACTGATCT GGTAGTCTGC GTGGCTCTTG GATGTGACAT GTTCGACTGC
GTCTTCCCCA CACGGACAGC GCGCTTTGGC TCTGCCCTGG TGCCCACTGG GAACCTGCAG
TTGAGGAAGA AGGTGTTTGA GAAGGACTTC GGCCCCATAG ACCCGGAGTG CACCTGCCCC
ACGTGCCAAA AGCACAGCCG CGCCTTCCTG CACGCACTGC TGCACAGTGA CAACACGGCC
GCGCTGCACC ACCTCACGGT CCACAACATC GCCTACCAGC TGCAGCTCAT GAGCGCCGTC
CGCACCAGCA TCGTGGAGAA GCGCTTCCCG GACTTCGTGC GGGACTTCAT GGGCGCCATG
TACGGGGATC CCACCCTCTG TCCCACCTGG GCCACTGACG CTCTGGCCTC TGTGGGAATC
ACACTGGGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_112486.1
CDS26..1237
Misc Feature(1)29..31(+)
Misc Feature(2)92..1177(+)
Misc Feature(3)101..1153(+)
Exon (1)1..268
Gene:QTRT1
Gene Synonym:
Exon (2)269..337
Gene:QTRT1
Gene Synonym:
Exon (3)338..476
Gene:QTRT1
Gene Synonym:
Exon (4)477..555
Gene:QTRT1
Gene Synonym:
Exon (5)556..671
Gene:QTRT1
Gene Synonym:
Exon (6)672..810
Gene:QTRT1
Gene Synonym:
Exon (7)811..886
Gene:QTRT1
Gene Synonym:
Exon (8)887..996
Gene:QTRT1
Gene Synonym:
Exon (9)997..1084
Gene:QTRT1
Gene Synonym:
Exon (10)1085..1334
Gene:QTRT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_031209
Organism Homo sapiens (human)
Definition Homo sapiens queuine tRNA-ribosyltransferase 1 (QTRT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_031209

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCGGGAG CAGCTACCCA GGCTTCCCTG GAGTCGGCCC CACGGATCAT GCGGCTGGTG 
GCCGAATGCA GCCGCTCCAG GGCCCGGGCA GGCGAGCTGT GGCTGCCGCA TGGGACAGTG
GCCACTCCTG TGTTCATGCC AGTGGGCACG CAGGCCACCA TGAAGGGCAT CACGACCGAA
CAGCTGGACG CTCTGGGTTG CCGCATCTGC CTGGGCAATA CCTACCATCT GGGTCTAAGG
CCGGGACCCG AGCTGATCCA GAAAGCCAAC GGTCTCCACG GCTTCATGAA TTGGCCTCAT
AATCTGCTAA CGGACAGCGG CGGTTTCCAG ATGGTGTCGC TGGTGTCTCT GTCCGAGGTG
ACGGAGGAGG GCGTCCGCTT CCGCTCCCCC TACGACGGCA ATGAGACCCT GCTGAGCCCG
GAGAAATCCG TGCAGATCCA GAATGCGCTG GGCTCGGACA TCATCATGCA GCTGGACGAC
GTGGTTAGCA GTACTGTGAC TGGGCCACGT GTGGAGGAGG CCATGTACAG GTCAATCCGC
TGGCTGGACC GGTGCATTGC AGCCCATCAG CGGCCGGACA AGCAGAACCT CTTCGCCATT
ATCCAGGGTG GGCTGGACGC AGATCTCCGG GCCACCTGCC TTGAAGAGAT GACCAAGCGA
GACGTGCCTG GCTTCGCCAT CGGGGGCCTG AGCGGGGGTG AGAGCAAGTC GCAGTTCTGG
CGGATGGTGG CGCTGAGCAC CTCTCGGCTG CCGAAGGACA AGCCCCGATA TCTGATGGGG
GTTGGCTATG CCACTGATCT GGTAGTCTGC GTGGCTCTTG GATGTGACAT GTTCGACTGC
GTCTTCCCCA CACGGACAGC GCGCTTTGGC TCTGCCCTGG TGCCCACTGG GAACCTGCAG
TTGAGGAAGA AGGTGTTTGA GAAGGACTTC GGCCCCATAG ACCCGGAGTG CACCTGCCCC
ACGTGCCAAA AGCACAGCCG CGCCTTCCTG CACGCACTGC TGCACAGTGA CAACACGGCC
GCGCTGCACC ACCTCACGGT CCACAACATC GCCTACCAGC TGCAGCTCAT GAGCGCCGTC
CGCACCAGCA TCGTGGAGAA GCGCTTCCCG GACTTCGTGC GGGACTTCAT GGGCGCCATG
TACGGGGATC CCACCCTCTG TCCCACCTGG GCCACTGACG CTCTGGCCTC TGTGGGAATC
ACACTGGGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Investigation of specificity determinants in bacterial tRNA-guanine transglycosylase reveals queuine, the substrate of its eucaryotic counterpart, as inhibitor
PLoS ONE 8 (5), E64240 (2013)
Biela I, Tidten-Luksch N, Immekus F, Glinca S, Nguyen TX, Gerber HD, Heine A, Klebe G and Reuter K.


book

Differential heterocyclic substrate recognition by, and pteridine inhibition of E. coli and human tRNA-guanine transglycosylases
Biochem. Biophys. Res. Commun. 410 (1), 34-39 (2011)
Eric Thomas C, Chen YC and Garcia GA.


book

Evolution of eukaryal tRNA-guanine transglycosylase: insight gained from the heterocyclic substrate recognition by the wild-type and mutant human and Escherichia coli tRNA-guanine transglycosylases
Nucleic Acids Res. 39 (7), 2834-2844 (2011)
Chen YC, Brooks AF, Goodenough-Lashua DM, Kittendorf JD, Showalter HD and Garcia GA.


book

Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure
RNA 16 (5), 958-968 (2010)
Chen YC, Kelly VP, Stachura SV and Garcia GA.


book

Characterization of cDNA encoding the human tRNA-guanine transglycosylase (TGT) catalytic subunit
Gene 265 (1-2), 205-212 (2001)
Deshpande KL and Katze JR.


 
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