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DYSF cDNA ORF clone, Homo sapiens (human)

Gene Symbol DYSF
Entrez Gene ID 8291
Full Name dysferlin
Synonyms FER1L1, LGMD2B, MMD1
General protein information
Preferred Names
dysferlin
Names
dysferlin
fer-1-like protein 1
dystrophy-associated fer-1-like 1
dystrophy-associated fer-1-like protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p13.3

Summary The protein encoded by this gene belongs to the ferlin family and is a skeletal muscle protein found associated with the sarcolemma. It is involved in muscle contraction and contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. In addition, the protein encoded by this gene binds caveolin-3, a skeletal muscle membrane protein which is important in the formation of caveolae. Specific mutations in this gene have been shown to cause autosomal recessive limb girdle muscular dystrophy type 2B (LGMD2B) as well as Miyoshi myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2008]. lac of sum
Disorder MIM:

603009

Disorder Html: Muscular dystrophy, limb-girdle, type 2B, 253601 (3); Miyoshi

mRNA and Protein(s)

mRNA Protein Name
XM_005264584 XP_005264641 dysferlin isoform X1
XM_005264585 XP_005264642 dysferlin isoform X2
NM_003494 NP_003485 dysferlin isoform 8
NM_001130455 NP_001123927 dysferlin isoform 2
NM_001130976 NP_001124448 dysferlin isoform 9
NM_001130977 NP_001124449 dysferlin isoform 10
NM_001130978 NP_001124450 dysferlin isoform 11
NM_001130979 NP_001124451 dysferlin isoform 12
NM_001130980 NP_001124452 dysferlin isoform 13
NM_001130981 NP_001124453 dysferlin isoform 14
NM_001130982 NP_001124454 dysferlin isoform 7
NM_001130983 NP_001124455 dysferlin isoform 6
NM_001130984 NP_001124456 dysferlin isoform 5
NM_001130985 NP_001124457 dysferlin isoform 4
NM_001130986 NP_001124458 dysferlin isoform 3
NM_001130987 NP_001124459 dysferlin isoform 1



Homo sapiens (human) DYSF NP_001124454.1
Pan troglodytes (chimpanzee) DYSF XP_003309136.1
Macaca mulatta (Rhesus monkey) DYSF XP_002799336.1
Canis lupus familiaris (dog) DYSF XP_003432278.1
Bos taurus (cattle) DYSF NP_001095960.1
Mus musculus (house mouse) Dysf NP_067444.2
Rattus norvegicus (Norway rat) Dysf NP_001101339.1
Gallus gallus (chicken) DYSF XP_423126.4
Danio rerio (zebrafish) dysf NP_001244122.1
Caenorhabditis elegans fer-1 NP_492337.1
Caenorhabditis elegans ferl-1 NP_491602.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DYSF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DYSF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu35620 XM_005264584 PREDICTED: Homo sapiens dysferlin (DYSF), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu35621 XM_005264585 PREDICTED: Homo sapiens dysferlin (DYSF), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu28618 NM_003494 Homo sapiens dysferlin (DYSF), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu28611 NM_001130455 Homo sapiens dysferlin (DYSF), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu28621 NM_001130976 Homo sapiens dysferlin (DYSF), transcript variant 9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu28606 NM_001130977 Homo sapiens dysferlin (DYSF), transcript variant 10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu28612 NM_001130978 Homo sapiens dysferlin (DYSF), transcript variant 11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu28614 NM_001130979 Homo sapiens dysferlin (DYSF), transcript variant 12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu28604 NM_001130980 Homo sapiens dysferlin (DYSF), transcript variant 13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu28599 NM_001130981 Homo sapiens dysferlin (DYSF), transcript variant 14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu28609 NM_001130982 Homo sapiens dysferlin (DYSF), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu28616 NM_001130983 Homo sapiens dysferlin (DYSF), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu28600 NM_001130984 Homo sapiens dysferlin (DYSF), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu28617 NM_001130985 Homo sapiens dysferlin (DYSF), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu28615 NM_001130986 Homo sapiens dysferlin (DYSF), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu28601 NM_001130987 Homo sapiens dysferlin (DYSF), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu35620
Clone ID Related Accession (Same CDS sequence) XM_005264584
Accession Version XM_005264584.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6402bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578803187. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT
CCCAGAGAGT TCACAGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT
GATGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGACATCCAT
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCATCACCTG
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT
GGGAAGCTAC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG CGCCCGGATG
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCAG TGGATGAGAA GGAGTTCAAC
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACTAAGTTG
GCCCTTGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACG
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG ACAAGAGTGA AGATTCCATG
TCCGTCTCCA CCTTGAGCTT CGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT
CTGGAACGGA TGCCACGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCGTCGGGG
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG
CTCCAGCGTA CCGCCATCGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTG GGGGCCAGAC GGTGCAGTCC
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC
AGTCCTCATG AGGAAGAGTT CATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG
AGGGAAAAGT GCGGCTCCTA CCTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TGATTGGTGA ATTTAAGGGC
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC
CACCAGCTGG CCGCCCAGGG ACCCCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA
TTTGGCCTGC AGCCCAAGGA CCCCAATGGA AAGTGTGATC CTTACATCAA GATCTCCATA
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGCTGGA GCCCGTATTT
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTAAA GATCACTCTC
TATGACTATG ACCTCCTCTC CAAGGACGAA AAGATCGGTG AGACGGTCGT CGACCTGGAG
AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT
GGACCGAACC AGTGGCGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG
CAGCATAGAG TCAAGGCACC TGTGTACCGG ACAGACCGTG TAATGTTTCA GGATAAAGAA
TATTCCATTG AAGAGATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCAGTGGAG
GAGCGTCTGG CTCTGCATGT GCTTCAGCAG CAGGGCCTGG TCCCGGAGCA CGTGGAGTCA
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC
GACCTATTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGTTCCC TGGGAGGTGA AGGCAACTTC
AACTGGAGGT TCATTTTCCC CTTCGACTAC CTGCCAGCTG AGCAAGTCTG TACCATTGCC
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGGTG
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCAAGA AGTGCTCCTT GGACCAGCTG
GATGATGCTT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC
TGGTGGCCCT GTGTAGCAGA AGAGGGTGAG AAGAAAATAC TGGCGGGCAA GCTGGAAATG
ACCTTGGAGA TTGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT
GAGCCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG
TTTACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC
ATCCTCTTCA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264641.1
CDS141..6542
Misc Feature(1)153..542(+)
Misc Feature(2)885..1214(+)
Misc Feature(3)1143..1358(+)
Misc Feature(4)1374..1898(+)
Misc Feature(5)1467..1679(+)
Misc Feature(6)2316..2513(+)
Misc Feature(7)2592..2819(+)
Misc Feature(8)2859..3035(+)
Misc Feature(9)3072..3242(+)
Misc Feature(10)3690..4076(+)
Misc Feature(11)3738..3932(+)
Misc Feature(12)5034..5405(+)
Misc Feature(13)5079..5267(+)
Misc Feature(14)5736..6131(+)
Translation

Target ORF information:

RefSeq Version XM_005264584
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264584

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT
CCCAGAGAGT TCACAGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT
GATGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGACATCCAT
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCATCACCTG
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT
GGGAAGCTAC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG CGCCCGGATG
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCAG TGGATGAGAA GGAGTTCAAC
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACTAAGTTG
GCCCTTGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACG
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG ACAAGAGTGA AGATTCCATG
TCCGTCTCCA CCTTGAGCTT CGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT
CTGGAACGGA TGCCACGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCGTCGGGG
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG
CTCCAGCGTA CCGCCATCGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTG GGGGCCAGAC GGTGCAGTCC
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCCACTA ACACGGCTTC TCCTCCATCC
AGTCCTCATG AGGAAGAGTT CATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG
AGGGAAAAGT GCGGCTCCTA CCTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TGATTGGTGA ATTTAAGGGC
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC
CACCAGCTGG CCGCCCAGGG ACCCCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA
TTTGGCCTGC AGCCCAAGGA CCCCAATGGA AAGTGTGATC CTTACATCAA GATCTCCATA
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGCTGGA GCCCGTATTT
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTAAA GATCACTCTC
TATGACTATG ACCTCCTCTC CAAGGACGAA AAGATCGGTG AGACGGTCGT CGACCTGGAG
AACAGGCTGC TGTCCAAGTT TGGGGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT
GGACCGAACC AGTGGCGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG
CAGCATAGAG TCAAGGCACC TGTGTACCGG ACAGACCGTG TAATGTTTCA GGATAAAGAA
TATTCCATTG AAGAGATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCAGTGGAG
GAGCGTCTGG CTCTGCATGT GCTTCAGCAG CAGGGCCTGG TCCCGGAGCA CGTGGAGTCA
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATCGAGCAGG GGAAGCTGCA GATGTGGGTC
GACCTATTTC CGAAGGCCCT GGGGCGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA
GCCAGAAGGT TTTTCCTGCG TTGTATTATC TGGAATACCA GAGATGTGAT CCTGGATGAC
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGTTCCC TGGGAGGTGA AGGCAACTTC
AACTGGAGGT TCATTTTCCC CTTCGACTAC CTGCCAGCTG AGCAAGTCTG TACCATTGCC
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGGTG
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCAAGA AGTGCTCCTT GGACCAGCTG
GATGATGCTT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC
TGGTGGCCCT GTGTAGCAGA AGAGGGTGAG AAGAAAATAC TGGCGGGCAA GCTGGAAATG
ACCTTGGAGA TTGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT
GAGCCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG
TTTACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC
ATCCTCTTCA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35621
Clone ID Related Accession (Same CDS sequence) XM_005264585
Accession Version XM_005264585.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6399bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578803188. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC
AGAGAGTTCA CAGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAT
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA CATCCATGAG
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCA TCACCTGAGC
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG
AAGCTACAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGCGC CCGGATGCCA
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCAGTGG ATGAGAAGGA GTTCAACCAG
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC TAAGTTGGCC
CTTGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACGGGC
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGACA AGAGTGAAGA TTCCATGTCC
GTCTCCACCT TGAGCTTCGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG
GAACGGATGC CACGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC GTCGGGGGAG
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC
CAGCGTACCG CCATCGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC
CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC CCCACTAACA CGGCTTCTCC TCCATCCAGT
CCTCATGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG
GAAAAGTGCG GCTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTGA TTGGTGAATT TAAGGGCCTC
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT
GGCCTGCAGC CCAAGGACCC CAATGGAAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264642.1
CDS881..7279
Misc Feature(1)938..1279(+)
Misc Feature(2)1622..1951(+)
Misc Feature(3)1880..2095(+)
Misc Feature(4)2111..2635(+)
Misc Feature(5)2204..2416(+)
Misc Feature(6)3053..3250(+)
Misc Feature(7)3329..3556(+)
Misc Feature(8)3596..3772(+)
Misc Feature(9)3809..3979(+)
Misc Feature(10)4427..4813(+)
Misc Feature(11)4475..4669(+)
Misc Feature(12)5771..6142(+)
Misc Feature(13)5816..6004(+)
Misc Feature(14)6473..6868(+)
Translation

Target ORF information:

RefSeq Version XM_005264585
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264585

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC
AGAGAGTTCA CAGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAT
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA CATCCATGAG
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCA TCACCTGAGC
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG
AAGCTACAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGCGC CCGGATGCCA
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCAGTGG ATGAGAAGGA GTTCAACCAG
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC TAAGTTGGCC
CTTGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACGGGC
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGACA AGAGTGAAGA TTCCATGTCC
GTCTCCACCT TGAGCTTCGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG
GAACGGATGC CACGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC GTCGGGGGAG
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC
CAGCGTACCG CCATCGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC
CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC CCCACTAACA CGGCTTCTCC TCCATCCAGT
CCTCATGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG
GAAAAGTGCG GCTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTGA TTGGTGAATT TAAGGGCCTC
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT
GGCCTGCAGC CCAAGGACCC CAATGGAAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28618
Clone ID Related Accession (Same CDS sequence) NM_003494
Accession Version NM_003494.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6243bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 8
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, AF075575.1, AI128455.1 and DB292971.1. This sequence is a reference standard in the RefSeqGene project. On Jul 24, 2008 this sequence version replaced gi:19743938. Transcript Variant: This variant (8) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (8) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF075575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACAG GCTTCCCAGA CCCCTACACA
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA
GCCACCATGC TGCAGGATGT GGATGATGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC
AGCCAGCCTC TGGGTGACAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG
TACCAGCTGC GCACCCATCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTACAGACAA TCTTTCTGAA ATATCCGATG
GAGAAGGTGC CTGGCGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC
TCAGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA
ACCTATGAGA ACGAGACTAA GTTGGCCCTT GTTGGGAACT GGGGCACAAC GGGCCTCACC
TACCCCAAGT TTTCTGACGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG
GATGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTCGGTGT GAACAGACCC
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCAC GGCTGGCCTG GTTCCCACTG
ACGAGGGGCA GCCAGCCGTC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGATCCT GGCATGGGGC
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TCATCGATTG GTGGAGCAAA
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACCTGGAGAA GGATTTTGAC
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT
GTGATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG AAAGTGTGAT
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT
GAGACGGTCG TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCAAG
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003485.1
CDS377..6619
Misc Feature(1)182..184(+)
Misc Feature(2)434..775(+)
Misc Feature(3)656..658(+)
Misc Feature(4)662..664(+)
Misc Feature(5)1025..1354(+)
Misc Feature(6)1283..1498(+)
Misc Feature(7)1514..2038(+)
Misc Feature(8)1607..1819(+)
Misc Feature(9)2456..2653(+)
Misc Feature(10)2732..2959(+)
Misc Feature(11)2999..3175(+)
Misc Feature(12)3212..3382(+)
Misc Feature(13)3830..4216(+)
Misc Feature(14)3878..4072(+)
Misc Feature(15)5111..5482(+)
Misc Feature(16)5156..5344(+)
Misc Feature(17)5813..6208(+)
Misc Feature(18)6515..6577(+)
Exon (1)1..464
Gene:DYSF
Gene Synonym:
Exon (2)465..520
Gene:DYSF
Gene Synonym:
Exon (3)521..612
Gene:DYSF
Gene Synonym:
Exon (4)613..718
Gene:DYSF
Gene Synonym:
Exon (5)719..833
Gene:DYSF
Gene Synonym:
Exon (6)834..1039
Gene:DYSF
Gene Synonym:
Exon (7)1040..1168
Gene:DYSF
Gene Synonym:
Exon (8)1169..1231
Gene:DYSF
Gene Synonym:
Exon (9)1232..1282
Gene:DYSF
Gene Synonym:
Exon (10)1283..1313
Gene:DYSF
Gene Synonym:
Exon (11)1314..1429
Gene:DYSF
Gene Synonym:
Exon (12)1430..1556
Gene:DYSF
Gene Synonym:
Exon (13)1557..1660
Gene:DYSF
Gene Synonym:
Exon (14)1661..1729
Gene:DYSF
Gene Synonym:
Exon (15)1730..1773
Gene:DYSF
Gene Synonym:
Exon (16)1774..1856
Gene:DYSF
Gene Synonym:
Exon (17)1857..1898
Gene:DYSF
Gene Synonym:
Exon (18)1899..2014
Gene:DYSF
Gene Synonym:
Exon (19)2015..2128
Gene:DYSF
Gene Synonym:
Exon (20)2129..2306
Gene:DYSF
Gene Synonym:
Exon (21)2307..2431
Gene:DYSF
Gene Synonym:
Exon (22)2432..2538
Gene:DYSF
Gene Synonym:
Exon (23)2539..2731
Gene:DYSF
Gene Synonym:
Exon (24)2732..2887
Gene:DYSF
Gene Synonym:
Exon (25)2888..3019
Gene:DYSF
Gene Synonym:
Exon (26)3020..3186
Gene:DYSF
Gene Synonym:
Exon (27)3187..3301
Gene:DYSF
Gene Synonym:
Exon (28)3302..3407
Gene:DYSF
Gene Synonym:
Exon (29)3408..3550
Gene:DYSF
Gene Synonym:
Exon (30)3551..3724
Gene:DYSF
Gene Synonym:
Exon (31)3725..3818
Gene:DYSF
Gene Synonym:
Exon (32)3819..3896
Gene:DYSF
Gene Synonym:
Exon (33)3897..4078
Gene:DYSF
Gene Synonym:
Exon (34)4079..4219
Gene:DYSF
Gene Synonym:
Exon (35)4220..4249
Gene:DYSF
Gene Synonym:
Exon (36)4250..4279
Gene:DYSF
Gene Synonym:
Exon (37)4280..4381
Gene:DYSF
Gene Synonym:
Exon (38)4382..4543
Gene:DYSF
Gene Synonym:
Exon (39)4544..4709
Gene:DYSF
Gene Synonym:
Exon (40)4710..4786
Gene:DYSF
Gene Synonym:
Exon (41)4787..4885
Gene:DYSF
Gene Synonym:
Exon (42)4886..5014
Gene:DYSF
Gene Synonym:
Exon (43)5015..5170
Gene:DYSF
Gene Synonym:
Exon (44)5171..5262
Gene:DYSF
Gene Synonym:
Exon (45)5263..5433
Gene:DYSF
Gene Synonym:
Exon (46)5434..5576
Gene:DYSF
Gene Synonym:
Exon (47)5577..5716
Gene:DYSF
Gene Synonym:
Exon (48)5717..5805
Gene:DYSF
Gene Synonym:
Exon (49)5806..5901
Gene:DYSF
Gene Synonym:
Exon (50)5902..6043
Gene:DYSF
Gene Synonym:
Exon (51)6044..6143
Gene:DYSF
Gene Synonym:
Exon (52)6144..6322
Gene:DYSF
Gene Synonym:
Exon (53)6323..6432
Gene:DYSF
Gene Synonym:
Exon (54)6433..6580
Gene:DYSF
Gene Synonym:
Exon (55)6581..6890
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003494
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003494

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACAG GCTTCCCAGA CCCCTACACA
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA
GCCACCATGC TGCAGGATGT GGATGATGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC
AGCCAGCCTC TGGGTGACAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG
TACCAGCTGC GCACCCATCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTACAGACAA TCTTTCTGAA ATATCCGATG
GAGAAGGTGC CTGGCGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC
TCAGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA
ACCTATGAGA ACGAGACTAA GTTGGCCCTT GTTGGGAACT GGGGCACAAC GGGCCTCACC
TACCCCAAGT TTTCTGACGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG
GATGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTCGGTGT GAACAGACCC
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCAC GGCTGGCCTG GTTCCCACTG
ACGAGGGGCA GCCAGCCGTC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGATCCT GGCATGGGGC
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TCATCGATTG GTGGAGCAAA
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACCTGGAGAA GGATTTTGAC
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT
GTGATTGGTG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCCCAGG GACCCCAGGA GTGCTTGGTC
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG AAAGTGTGAT
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC
TGCACGCTGG AGCCCGTATT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG
AAGGACCTAA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAGGACGA AAAGATCGGT
GAGACGGTCG TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGGGGCTCG CTGTGGACTC
CCACAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG
CTCCTCCACC TCTTCTGCCA GCAGCATAGA GTCAAGGCAC CTGTGTACCG GACAGACCGT
GTAATGTTTC AGGATAAAGA ATATTCCATT GAAGAGATAG AGGCTGGCAG GATCCCAAAC
CCACACCTGG GCCCAGTGGA GGAGCGTCTG GCTCTGCATG TGCTTCAGCA GCAGGGCCTG
GTCCCGGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATCGAGCAG
GGGAAGCTGC AGATGTGGGT CGACCTATTT CCGAAGGCCC TGGGGCGGCC TGGACCTCCC
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACC
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGTTCC
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCAGCT
GAGCAAGTCT GTACCATTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC
AAAATCCCAG CACGAGTGGT GTTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCAAG
AAGTGCTCCT TGGACCAGCT GGATGATGCT TTCCACCCAG AATGGTTTGT GTCCCTTTTT
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTGTAGCAG AAGAGGGTGA GAAGAAAATA
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAGCAG AGAGTGAGCA TGAGGAGCGG
CCTGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC
CCCGACACCT CCTTCCTGTG GTTTACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28611
Clone ID Related Accession (Same CDS sequence) NM_001130455
Accession Version NM_001130455.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6246bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ267935.1, AF075575.1 and DB292971.1. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ267935.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CAGGCTTCCC AGACCCCTAC
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC
TCAGCCACCA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TTGAGGTCAG CATCGGGAAC
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC
TGCAGCCAGC CTCTGGGTGA CATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC
CTGTACCAGC TGCGCACCCA TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTACAGA CAATCTTTCT GAAATATCCG
ATGGAGAAGG TGCCTGGCGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG
CTCTCAGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT
GAAACCTATG AGAACGAGAC TAAGTTGGCC CTTGTTGGGA ACTGGGGCAC AACGGGCCTC
ACCTACCCCA AGTTTTCTGA CGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG
ATGGATGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTCGG TGTGAACAGA
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CACGGCTGGC CTGGTTCCCA
CTGACGAGGG GCAGCCAGCC GTCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGAT CCTGGCATGG
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTCATCGA TTGGTGGAGC
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACCTGGA GAAGGATTTT
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA
TCTGTGATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGAAAGTGT
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC
GGTGAGACGG TCGTCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC
AAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC
AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001123927.1
CDS142..6387
Misc Feature(1)154..543(+)
Misc Feature(2)793..1122(+)
Misc Feature(3)1051..1266(+)
Misc Feature(4)1282..1806(+)
Misc Feature(5)1375..1587(+)
Misc Feature(6)2224..2421(+)
Misc Feature(7)2500..2727(+)
Misc Feature(8)2767..2943(+)
Misc Feature(9)2980..3150(+)
Misc Feature(10)3598..3984(+)
Misc Feature(11)3646..3840(+)
Misc Feature(12)4879..5250(+)
Misc Feature(13)4924..5112(+)
Misc Feature(14)5581..5976(+)
Misc Feature(15)6283..6345(+)
Exon (1)1..232
Gene:DYSF
Gene Synonym:
Exon (2)233..288
Gene:DYSF
Gene Synonym:
Exon (3)289..380
Gene:DYSF
Gene Synonym:
Exon (4)381..486
Gene:DYSF
Gene Synonym:
Exon (5)487..601
Gene:DYSF
Gene Synonym:
Exon (6)602..807
Gene:DYSF
Gene Synonym:
Exon (7)808..936
Gene:DYSF
Gene Synonym:
Exon (8)937..999
Gene:DYSF
Gene Synonym:
Exon (9)1000..1050
Gene:DYSF
Gene Synonym:
Exon (10)1051..1081
Gene:DYSF
Gene Synonym:
Exon (11)1082..1197
Gene:DYSF
Gene Synonym:
Exon (12)1198..1324
Gene:DYSF
Gene Synonym:
Exon (13)1325..1428
Gene:DYSF
Gene Synonym:
Exon (14)1429..1497
Gene:DYSF
Gene Synonym:
Exon (15)1498..1541
Gene:DYSF
Gene Synonym:
Exon (16)1542..1624
Gene:DYSF
Gene Synonym:
Exon (17)1625..1666
Gene:DYSF
Gene Synonym:
Exon (18)1667..1782
Gene:DYSF
Gene Synonym:
Exon (19)1783..1896
Gene:DYSF
Gene Synonym:
Exon (20)1897..2074
Gene:DYSF
Gene Synonym:
Exon (21)2075..2199
Gene:DYSF
Gene Synonym:
Exon (22)2200..2306
Gene:DYSF
Gene Synonym:
Exon (23)2307..2499
Gene:DYSF
Gene Synonym:
Exon (24)2500..2655
Gene:DYSF
Gene Synonym:
Exon (25)2656..2787
Gene:DYSF
Gene Synonym:
Exon (26)2788..2954
Gene:DYSF
Gene Synonym:
Exon (27)2955..3069
Gene:DYSF
Gene Synonym:
Exon (28)3070..3175
Gene:DYSF
Gene Synonym:
Exon (29)3176..3318
Gene:DYSF
Gene Synonym:
Exon (30)3319..3492
Gene:DYSF
Gene Synonym:
Exon (31)3493..3586
Gene:DYSF
Gene Synonym:
Exon (32)3587..3664
Gene:DYSF
Gene Synonym:
Exon (33)3665..3846
Gene:DYSF
Gene Synonym:
Exon (34)3847..3987
Gene:DYSF
Gene Synonym:
Exon (35)3988..4017
Gene:DYSF
Gene Synonym:
Exon (36)4018..4047
Gene:DYSF
Gene Synonym:
Exon (37)4048..4149
Gene:DYSF
Gene Synonym:
Exon (38)4150..4311
Gene:DYSF
Gene Synonym:
Exon (39)4312..4477
Gene:DYSF
Gene Synonym:
Exon (40)4478..4554
Gene:DYSF
Gene Synonym:
Exon (41)4555..4653
Gene:DYSF
Gene Synonym:
Exon (42)4654..4782
Gene:DYSF
Gene Synonym:
Exon (43)4783..4938
Gene:DYSF
Gene Synonym:
Exon (44)4939..5030
Gene:DYSF
Gene Synonym:
Exon (45)5031..5201
Gene:DYSF
Gene Synonym:
Exon (46)5202..5344
Gene:DYSF
Gene Synonym:
Exon (47)5345..5484
Gene:DYSF
Gene Synonym:
Exon (48)5485..5573
Gene:DYSF
Gene Synonym:
Exon (49)5574..5669
Gene:DYSF
Gene Synonym:
Exon (50)5670..5811
Gene:DYSF
Gene Synonym:
Exon (51)5812..5911
Gene:DYSF
Gene Synonym:
Exon (52)5912..6090
Gene:DYSF
Gene Synonym:
Exon (53)6091..6200
Gene:DYSF
Gene Synonym:
Exon (54)6201..6348
Gene:DYSF
Gene Synonym:
Exon (55)6349..6658
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130455
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130455

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CAGGCTTCCC AGACCCCTAC
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC
TCAGCCACCA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TTGAGGTCAG CATCGGGAAC
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC
TGCAGCCAGC CTCTGGGTGA CATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC
CTGTACCAGC TGCGCACCCA TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTACAGA CAATCTTTCT GAAATATCCG
ATGGAGAAGG TGCCTGGCGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG
CTCTCAGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT
GAAACCTATG AGAACGAGAC TAAGTTGGCC CTTGTTGGGA ACTGGGGCAC AACGGGCCTC
ACCTACCCCA AGTTTTCTGA CGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG
ATGGATGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTCGG TGTGAACAGA
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CACGGCTGGC CTGGTTCCCA
CTGACGAGGG GCAGCCAGCC GTCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGAT CCTGGCATGG
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTCATCGA TTGGTGGAGC
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACCTGGA GAAGGATTTT
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA
TCTGTGATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGAAAGTGT
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC
GGTGAGACGG TCGTCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC
AAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC
AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28621
Clone ID Related Accession (Same CDS sequence) NM_001130976
Accession Version NM_001130976.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6201bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 9
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, EU515156.1, AI128455.1, DB292971.1 and AK074649.1. Transcript Variant: This variant (9) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (9) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515156.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACAGGC
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGATGCCAT CCAGTTTGAG
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC
ACTCAGTACA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAC
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGACATCC ATGAGACACC CTCTGCCACC
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCATCACC TGAGCCAAAT CACTGAGGCT
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT ACAGACAATC
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGCGCCCGGA TGCCAGTGCA GATACGGGTC
AAGCTGTGGT TTGGGCTCTC AGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACTAAGT TGGCCCTTGT TGGGAACTGG
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CGGGCAAGAT CAAGCTACCC
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG
GCCCTGGGCG GCGTGATGGA TGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC
TTCGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCACGG
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCGTCGG GGGAGCTGCT GGCCTCTTTT
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC
CCCAGCCTCG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC
CTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG
GAGACAGAAG ATCCATCTGT GATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC
CCCAATGGAA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC
AAGGACGAAA AGATCGGTGA GACGGTCGTC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG
CCAGCCAAGA CAGCCAAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124448.1
CDS377..6577
Misc Feature(1)182..184(+)
Misc Feature(2)434..775(+)
Misc Feature(3)1025..1354(+)
Misc Feature(4)1283..1498(+)
Misc Feature(5)1514..1996(+)
Misc Feature(6)1607..1819(+)
Misc Feature(7)2414..2611(+)
Misc Feature(8)2690..2917(+)
Misc Feature(9)2957..3133(+)
Misc Feature(10)3170..3340(+)
Misc Feature(11)3788..4174(+)
Misc Feature(12)3836..4030(+)
Misc Feature(13)5069..5440(+)
Misc Feature(14)5114..5302(+)
Misc Feature(15)5771..6166(+)
Misc Feature(16)6473..6535(+)
Exon (1)1..464
Gene:DYSF
Gene Synonym:
Exon (2)465..520
Gene:DYSF
Gene Synonym:
Exon (3)521..612
Gene:DYSF
Gene Synonym:
Exon (4)613..718
Gene:DYSF
Gene Synonym:
Exon (5)719..833
Gene:DYSF
Gene Synonym:
Exon (6)834..1039
Gene:DYSF
Gene Synonym:
Exon (7)1040..1168
Gene:DYSF
Gene Synonym:
Exon (8)1169..1231
Gene:DYSF
Gene Synonym:
Exon (9)1232..1282
Gene:DYSF
Gene Synonym:
Exon (10)1283..1313
Gene:DYSF
Gene Synonym:
Exon (11)1314..1429
Gene:DYSF
Gene Synonym:
Exon (12)1430..1556
Gene:DYSF
Gene Synonym:
Exon (13)1557..1660
Gene:DYSF
Gene Synonym:
Exon (14)1661..1729
Gene:DYSF
Gene Synonym:
Exon (15)1730..1773
Gene:DYSF
Gene Synonym:
Exon (16)1774..1856
Gene:DYSF
Gene Synonym:
Exon (17)1857..1972
Gene:DYSF
Gene Synonym:
Exon (18)1973..2086
Gene:DYSF
Gene Synonym:
Exon (19)2087..2264
Gene:DYSF
Gene Synonym:
Exon (20)2265..2389
Gene:DYSF
Gene Synonym:
Exon (21)2390..2496
Gene:DYSF
Gene Synonym:
Exon (22)2497..2689
Gene:DYSF
Gene Synonym:
Exon (23)2690..2845
Gene:DYSF
Gene Synonym:
Exon (24)2846..2977
Gene:DYSF
Gene Synonym:
Exon (25)2978..3144
Gene:DYSF
Gene Synonym:
Exon (26)3145..3259
Gene:DYSF
Gene Synonym:
Exon (27)3260..3365
Gene:DYSF
Gene Synonym:
Exon (28)3366..3508
Gene:DYSF
Gene Synonym:
Exon (29)3509..3682
Gene:DYSF
Gene Synonym:
Exon (30)3683..3776
Gene:DYSF
Gene Synonym:
Exon (31)3777..3854
Gene:DYSF
Gene Synonym:
Exon (32)3855..4036
Gene:DYSF
Gene Synonym:
Exon (33)4037..4177
Gene:DYSF
Gene Synonym:
Exon (34)4178..4207
Gene:DYSF
Gene Synonym:
Exon (35)4208..4237
Gene:DYSF
Gene Synonym:
Exon (36)4238..4339
Gene:DYSF
Gene Synonym:
Exon (37)4340..4501
Gene:DYSF
Gene Synonym:
Exon (38)4502..4667
Gene:DYSF
Gene Synonym:
Exon (39)4668..4744
Gene:DYSF
Gene Synonym:
Exon (40)4745..4843
Gene:DYSF
Gene Synonym:
Exon (41)4844..4972
Gene:DYSF
Gene Synonym:
Exon (42)4973..5128
Gene:DYSF
Gene Synonym:
Exon (43)5129..5220
Gene:DYSF
Gene Synonym:
Exon (44)5221..5391
Gene:DYSF
Gene Synonym:
Exon (45)5392..5534
Gene:DYSF
Gene Synonym:
Exon (46)5535..5674
Gene:DYSF
Gene Synonym:
Exon (47)5675..5763
Gene:DYSF
Gene Synonym:
Exon (48)5764..5859
Gene:DYSF
Gene Synonym:
Exon (49)5860..6001
Gene:DYSF
Gene Synonym:
Exon (50)6002..6101
Gene:DYSF
Gene Synonym:
Exon (51)6102..6280
Gene:DYSF
Gene Synonym:
Exon (52)6281..6390
Gene:DYSF
Gene Synonym:
Exon (53)6391..6538
Gene:DYSF
Gene Synonym:
Exon (54)6539..6848
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130976
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130976

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACAGGC
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGATGCCAT CCAGTTTGAG
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC
ACTCAGTACA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAC
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGACATCC ATGAGACACC CTCTGCCACC
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCATCACC TGAGCCAAAT CACTGAGGCT
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT ACAGACAATC
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGCGCCCGGA TGCCAGTGCA GATACGGGTC
AAGCTGTGGT TTGGGCTCTC AGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACTAAGT TGGCCCTTGT TGGGAACTGG
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CGGGCAAGAT CAAGCTACCC
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG
GCCCTGGGCG GCGTGATGGA TGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC
TTCGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCACGG
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCGTCGG GGGAGCTGCT GGCCTCTTTT
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC
CCCAGCCTCG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTC
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC
CTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG
GAGACAGAAG ATCCATCTGT GATTGGTGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCCCAGGGA
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC
CCCAATGGAA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC
CAGGATAACT ACATCCCCTG CACGCTGGAG CCCGTATTTG GAAAGATGTT CGAGCTGACC
TGCACTCTGC CTCTGGAGAA GGACCTAAAG ATCACTCTCT ATGACTATGA CCTCCTCTCC
AAGGACGAAA AGATCGGTGA GACGGTCGTC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT
GGGGCTCGCT GTGGACTCCC ACAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCATAGAGT CAAGGCACCT
GTGTACCGGA CAGACCGTGT AATGTTTCAG GATAAAGAAT ATTCCATTGA AGAGATAGAG
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCAGTGGAGG AGCGTCTGGC TCTGCATGTG
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG
CAGCCAGACA TCGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTATTTCC GAAGGCCCTG
GGGCGGCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT
TGTATTATCT GGAATACCAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA
GACGTGCATT ATCGTTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC
TTCGACTACC TGCCAGCTGA GCAAGTCTGT ACCATTGCCA AGAAGGATGC CTTCTGGAGG
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGGTGT TCCAGATCTG GGACAATGAC
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG
CCAGCCAAGA CAGCCAAGAA GTGCTCCTTG GACCAGCTGG ATGATGCTTT CCACCCAGAA
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TGTAGCAGAA
GAGGGTGAGA AGAAAATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAGCAGAG
AGTGAGCATG AGGAGCGGCC TGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TTACCTCCCC ATACAAGACC
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28606
Clone ID Related Accession (Same CDS sequence) NM_001130977
Accession Version NM_001130977.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6264bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 10
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, EU515159.1, AI739271.1, DB292971.1 and AK074649.1. Transcript Variant: This variant (10) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (10) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACAGGC
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGATGCCAT CCAGTTTGAG
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC
ACTCAGTACA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAC
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGACATCC ATGAGACACC CTCTGCCACC
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCATCACC TGAGCCAAAT CACTGAGGCT
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT ACAGACAATC
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGCGCCCGGA TGCCAGTGCA GATACGGGTC
AAGCTGTGGT TTGGGCTCTC AGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACTAAGT TGGCCCTTGT TGGGAACTGG
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CGGGCAAGAT CAAGCTACCC
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG
GCCCTGGGCG GCGTGATGGA TGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC
TTCGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCACGG
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCGTCGG GGGAGCTGCT GGCCTCTTTT
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC
CCCAGCCTCG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG
GAGGAGACAG AAGATCCATC TGTGATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG
GACCCCAATG GAAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC
TCCAAGGACG AAAAGATCGG TGAGACGGTC GTCGACCTGG AGAACAGGCT GCTGTCCAAG
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC
AAGCCAGCCA AGACAGCCAA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124449.1
CDS377..6640
Misc Feature(1)182..184(+)
Misc Feature(2)434..775(+)
Misc Feature(3)1025..1354(+)
Misc Feature(4)1283..1498(+)
Misc Feature(5)1514..1996(+)
Misc Feature(6)1607..1819(+)
Misc Feature(7)2414..2611(+)
Misc Feature(8)2690..2917(+)
Misc Feature(9)2957..3133(+)
Misc Feature(10)3170..3340(+)
Misc Feature(11)3788..4174(+)
Misc Feature(12)3836..4030(+)
Misc Feature(13)5132..5503(+)
Misc Feature(14)5177..5365(+)
Misc Feature(15)5834..6229(+)
Misc Feature(16)6536..6598(+)
Exon (1)1..464
Gene:DYSF
Gene Synonym:
Exon (2)465..520
Gene:DYSF
Gene Synonym:
Exon (3)521..612
Gene:DYSF
Gene Synonym:
Exon (4)613..718
Gene:DYSF
Gene Synonym:
Exon (5)719..833
Gene:DYSF
Gene Synonym:
Exon (6)834..1039
Gene:DYSF
Gene Synonym:
Exon (7)1040..1168
Gene:DYSF
Gene Synonym:
Exon (8)1169..1231
Gene:DYSF
Gene Synonym:
Exon (9)1232..1282
Gene:DYSF
Gene Synonym:
Exon (10)1283..1313
Gene:DYSF
Gene Synonym:
Exon (11)1314..1429
Gene:DYSF
Gene Synonym:
Exon (12)1430..1556
Gene:DYSF
Gene Synonym:
Exon (13)1557..1660
Gene:DYSF
Gene Synonym:
Exon (14)1661..1729
Gene:DYSF
Gene Synonym:
Exon (15)1730..1773
Gene:DYSF
Gene Synonym:
Exon (16)1774..1856
Gene:DYSF
Gene Synonym:
Exon (17)1857..1972
Gene:DYSF
Gene Synonym:
Exon (18)1973..2086
Gene:DYSF
Gene Synonym:
Exon (19)2087..2264
Gene:DYSF
Gene Synonym:
Exon (20)2265..2389
Gene:DYSF
Gene Synonym:
Exon (21)2390..2496
Gene:DYSF
Gene Synonym:
Exon (22)2497..2689
Gene:DYSF
Gene Synonym:
Exon (23)2690..2845
Gene:DYSF
Gene Synonym:
Exon (24)2846..2977
Gene:DYSF
Gene Synonym:
Exon (25)2978..3144
Gene:DYSF
Gene Synonym:
Exon (26)3145..3259
Gene:DYSF
Gene Synonym:
Exon (27)3260..3365
Gene:DYSF
Gene Synonym:
Exon (28)3366..3508
Gene:DYSF
Gene Synonym:
Exon (29)3509..3682
Gene:DYSF
Gene Synonym:
Exon (30)3683..3776
Gene:DYSF
Gene Synonym:
Exon (31)3777..3854
Gene:DYSF
Gene Synonym:
Exon (32)3855..4036
Gene:DYSF
Gene Synonym:
Exon (33)4037..4177
Gene:DYSF
Gene Synonym:
Exon (34)4178..4207
Gene:DYSF
Gene Synonym:
Exon (35)4208..4237
Gene:DYSF
Gene Synonym:
Exon (36)4238..4339
Gene:DYSF
Gene Synonym:
Exon (37)4340..4501
Gene:DYSF
Gene Synonym:
Exon (38)4502..4667
Gene:DYSF
Gene Synonym:
Exon (39)4668..4744
Gene:DYSF
Gene Synonym:
Exon (40)4745..4807
Gene:DYSF
Gene Synonym:
Exon (41)4808..4906
Gene:DYSF
Gene Synonym:
Exon (42)4907..5035
Gene:DYSF
Gene Synonym:
Exon (43)5036..5191
Gene:DYSF
Gene Synonym:
Exon (44)5192..5283
Gene:DYSF
Gene Synonym:
Exon (45)5284..5454
Gene:DYSF
Gene Synonym:
Exon (46)5455..5597
Gene:DYSF
Gene Synonym:
Exon (47)5598..5737
Gene:DYSF
Gene Synonym:
Exon (48)5738..5826
Gene:DYSF
Gene Synonym:
Exon (49)5827..5922
Gene:DYSF
Gene Synonym:
Exon (50)5923..6064
Gene:DYSF
Gene Synonym:
Exon (51)6065..6164
Gene:DYSF
Gene Synonym:
Exon (52)6165..6343
Gene:DYSF
Gene Synonym:
Exon (53)6344..6453
Gene:DYSF
Gene Synonym:
Exon (54)6454..6601
Gene:DYSF
Gene Synonym:
Exon (55)6602..6911
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130977
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130977

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACTA CCTGGGCTTC
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACAGGC
TTCCCAGACC CCTACACAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC
CGGCTTCTGC TCTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAACAGAA GGTGGAGGAC
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG
TTTGCGGCCT TCTACTCAGC CACCATGCTG CAGGATGTGG ATGATGCCAT CCAGTTTGAG
GTCAGCATCG GGAACTACGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC
ACTCAGTACA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGTAAC
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCGC TGGTGGCTCA GCTGACGGAT
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGACATCC ATGAGACACC CTCTGCCACC
CACCTGGACC AGTACCTGTA CCAGCTGCGC ACCCATCACC TGAGCCAAAT CACTGAGGCT
GCCCTGGCCC TGAAGCTCGG CCACAGTGAG CTCCCTGCAG CTCTGGAGCA GGCGGAGGAC
TGGCTCCTGC GTCTGCGTGC CCTGGCAGAG GAGCCCCAGA ACAGCCTGCC GGACATCGTC
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGCT ACAGACAATC
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGCGCCCGGA TGCCAGTGCA GATACGGGTC
AAGCTGTGGT TTGGGCTCTC AGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC GAGACTAAGT TGGCCCTTGT TGGGAACTGG
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CGGGCAAGAT CAAGCTACCC
AAGGACAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG
GAGAAGACTC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTCGTGGA AGAGGTGTTT
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT
GTGAACGGGG AGAAGGTGCT TCCCAAGGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT
AGCATCACCA TCCCCCCGGA GCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAAATGGAA
GCACTGAAAA GGCACAGGCA GGCGGAGGCG GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG
CGCCGTCGCA TGGAGCCACT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTTGAGGGG
GCCCTGGGCG GCGTGATGGA TGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC
TTCGGTGTGA ACAGACCCAC GATTTCCTGC ATATTCGACT ATGGGAACCG CTACCATCTA
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT TGGCGAGCCG
GCCACAGTTG CTGAGCAACC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAT
GGTGCAGACG AGTTTATGGG TCGCTGCATC TGTCAACCGA GTCTGGAACG GATGCCACGG
CTGGCCTGGT TCCCACTGAC GAGGGGCAGC CAGCCGTCGG GGGAGCTGCT GGCCTCTTTT
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG
ACATCAAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC
GAGATCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAA CATCTCCTCC
CCCAGCCTCG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC
CGGAAGAACC CCAACTTTGA CATCTGCACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG
GAGCTCTACT GCCCCCCCAT CACCGTCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT
CTGTCGAGCT TGGCCCCCAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG
GAGGAGACAG AAGATCCATC TGTGATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG
GACCCCAATG GAAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC
TCCAAGGACG AAAAGATCGG TGAGACGGTC GTCGACCTGG AGAACAGGCT GCTGTCCAAG
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC
AAGCCAGCCA AGACAGCCAA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28612
Clone ID Related Accession (Same CDS sequence) NM_001130978
Accession Version NM_001130978.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6306bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 11
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, EU515157.1, AI128455.1, DB292971.1 and AK074649.1. Transcript Variant: This variant (11) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (11) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515157.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACAG GCTTCCCAGA CCCCTACACA
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA
GCCACCATGC TGCAGGATGT GGATGATGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC
AGCCAGCCTC TGGGTGACAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG
TACCAGCTGC GCACCCATCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTACAGACAA TCTTTCTGAA ATATCCGATG
GAGAAGGTGC CTGGCGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC
TCAGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA
ACCTATGAGA ACGAGACTAA GTTGGCCCTT GTTGGGAACT GGGGCACAAC GGGCCTCACC
TACCCCAAGT TTTCTGACGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG
GATGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTCGGTGT GAACAGACCC
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCAC GGCTGGCCTG GTTCCCACTG
ACGAGGGGCA GCCAGCCGTC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGATCCT GGCATGGGGC
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCC
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA TTGGTGGAGC
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACCTGGA GAAGGATTTT
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA
TCTGTGATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGAAAGTGT
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC
GGTGAGACGG TCGTCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC
AAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC
AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124450.1
CDS377..6682
Misc Feature(1)182..184(+)
Misc Feature(2)434..775(+)
Misc Feature(3)1025..1354(+)
Misc Feature(4)1283..1498(+)
Misc Feature(5)1514..2038(+)
Misc Feature(6)1607..1819(+)
Misc Feature(7)2456..2653(+)
Misc Feature(8)2732..2959(+)
Misc Feature(9)2999..3175(+)
Misc Feature(10)3212..3382(+)
Misc Feature(11)3830..4216(+)
Misc Feature(12)3878..4072(+)
Misc Feature(13)5174..5545(+)
Misc Feature(14)5219..5407(+)
Misc Feature(15)5876..6271(+)
Misc Feature(16)6578..6640(+)
Exon (1)1..464
Gene:DYSF
Gene Synonym:
Exon (2)465..520
Gene:DYSF
Gene Synonym:
Exon (3)521..612
Gene:DYSF
Gene Synonym:
Exon (4)613..718
Gene:DYSF
Gene Synonym:
Exon (5)719..833
Gene:DYSF
Gene Synonym:
Exon (6)834..1039
Gene:DYSF
Gene Synonym:
Exon (7)1040..1168
Gene:DYSF
Gene Synonym:
Exon (8)1169..1231
Gene:DYSF
Gene Synonym:
Exon (9)1232..1282
Gene:DYSF
Gene Synonym:
Exon (10)1283..1313
Gene:DYSF
Gene Synonym:
Exon (11)1314..1429
Gene:DYSF
Gene Synonym:
Exon (12)1430..1556
Gene:DYSF
Gene Synonym:
Exon (13)1557..1660
Gene:DYSF
Gene Synonym:
Exon (14)1661..1729
Gene:DYSF
Gene Synonym:
Exon (15)1730..1773
Gene:DYSF
Gene Synonym:
Exon (16)1774..1856
Gene:DYSF
Gene Synonym:
Exon (17)1857..1898
Gene:DYSF
Gene Synonym:
Exon (18)1899..2014
Gene:DYSF
Gene Synonym:
Exon (19)2015..2128
Gene:DYSF
Gene Synonym:
Exon (20)2129..2306
Gene:DYSF
Gene Synonym:
Exon (21)2307..2431
Gene:DYSF
Gene Synonym:
Exon (22)2432..2538
Gene:DYSF
Gene Synonym:
Exon (23)2539..2731
Gene:DYSF
Gene Synonym:
Exon (24)2732..2887
Gene:DYSF
Gene Synonym:
Exon (25)2888..3019
Gene:DYSF
Gene Synonym:
Exon (26)3020..3186
Gene:DYSF
Gene Synonym:
Exon (27)3187..3301
Gene:DYSF
Gene Synonym:
Exon (28)3302..3407
Gene:DYSF
Gene Synonym:
Exon (29)3408..3550
Gene:DYSF
Gene Synonym:
Exon (30)3551..3724
Gene:DYSF
Gene Synonym:
Exon (31)3725..3818
Gene:DYSF
Gene Synonym:
Exon (32)3819..3896
Gene:DYSF
Gene Synonym:
Exon (33)3897..4078
Gene:DYSF
Gene Synonym:
Exon (34)4079..4219
Gene:DYSF
Gene Synonym:
Exon (35)4220..4249
Gene:DYSF
Gene Synonym:
Exon (36)4250..4279
Gene:DYSF
Gene Synonym:
Exon (37)4280..4381
Gene:DYSF
Gene Synonym:
Exon (38)4382..4543
Gene:DYSF
Gene Synonym:
Exon (39)4544..4709
Gene:DYSF
Gene Synonym:
Exon (40)4710..4786
Gene:DYSF
Gene Synonym:
Exon (41)4787..4849
Gene:DYSF
Gene Synonym:
Exon (42)4850..4948
Gene:DYSF
Gene Synonym:
Exon (43)4949..5077
Gene:DYSF
Gene Synonym:
Exon (44)5078..5233
Gene:DYSF
Gene Synonym:
Exon (45)5234..5325
Gene:DYSF
Gene Synonym:
Exon (46)5326..5496
Gene:DYSF
Gene Synonym:
Exon (47)5497..5639
Gene:DYSF
Gene Synonym:
Exon (48)5640..5779
Gene:DYSF
Gene Synonym:
Exon (49)5780..5868
Gene:DYSF
Gene Synonym:
Exon (50)5869..5964
Gene:DYSF
Gene Synonym:
Exon (51)5965..6106
Gene:DYSF
Gene Synonym:
Exon (52)6107..6206
Gene:DYSF
Gene Synonym:
Exon (53)6207..6385
Gene:DYSF
Gene Synonym:
Exon (54)6386..6495
Gene:DYSF
Gene Synonym:
Exon (55)6496..6643
Gene:DYSF
Gene Synonym:
Exon (56)6644..6953
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130978
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130978

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA
GAGGACCAGG GACTCACTGG AGATGAGGCG GAGCCATTCC TGGATCAAAG CGGAGGCCCG
GGGGCTCCCA CCACCCCAAG GAAACTACCT TCACGTCCTC CGCCCCACTA CCCCGGGATC
AAAAGAAAGC GAAGTGCGCC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGATTTC
CAGATCAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCGG GGGTGAACAT CAAGCCTGTG
GTCAAGGTTA CCGCTGCAGG GCAGACCAAG CGGACGCGGA TCCACAAGGG AAACAGCCCA
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTCTC CTGGGGAGCT GTTTGATGAG
CCCATCTTTA TCACGGTGGT AGACTCTCGT TCTCTCAGGA CAGATGCTCT CCTCGGGGAG
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGCTACCT GAAAACAAGC
CTTTGTGTGC TGGGGCCTGG GGACGAAGCG CCTCTGGAGA GAAAAGACCC CTCTGAAGAC
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TAGCCCTGCG AGGAGCCCAC
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCGCAGA TGGACGATGC CGTGATGGAC
AACGTGAAAC AGATCTTTGG CTTCGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG
GAGGTCAGCT TTGCGGGGAA AATGCTGTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGCGA AAAAATGAGG
ATTCGTATCA TAGACTGGGA CCGCCTGACT CACAATGACA TCGTGGCTAC CACCTACCTG
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGCCTGC AGGTGCTGTC
AAGCCTTCGA AAGCCTCAGA CTTGGATGAC TACCTGGGCT TCCTCCCCAC TTTTGGGCCC
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACAG GCTTCCCAGA CCCCTACACA
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTCTCCCTG
GAGACCAAGC TGGTGGAGCA CAGTGAACAG AAGGTGGAGG ACCTTCCTGC GGATGACATC
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCA
GCCACCATGC TGCAGGATGT GGATGATGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAC
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA CAGCCGTGCA
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGTA ACGTGAAACC TGTGGTGGTG
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC
TCCACGGAGG ACGTGGACTC GCTGGTGGCT CAGCTGACGG ATGAGCTCAT CGCAGGCTGC
AGCCAGCCTC TGGGTGACAT CCATGAGACA CCCTCTGCCA CCCACCTGGA CCAGTACCTG
TACCAGCTGC GCACCCATCA CCTGAGCCAA ATCACTGAGG CTGCCCTGGC CCTGAAGCTC
GGCCACAGTG AGCTCCCTGC AGCTCTGGAG CAGGCGGAGG ACTGGCTCCT GCGTCTGCGT
GCCCTGGCAG AGGAGCCCCA GAACAGCCTG CCGGACATCG TCATCTGGAT GCTGCAGGGA
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT
GCCAACTACT GTGGCAAGAA TTGTGGGAAG CTACAGACAA TCTTTCTGAA ATATCCGATG
GAGAAGGTGC CTGGCGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC
TCAGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA
ACCTATGAGA ACGAGACTAA GTTGGCCCTT GTTGGGAACT GGGGCACAAC GGGCCTCACC
TACCCCAAGT TTTCTGACGT CACGGGCAAG ATCAAGCTAC CCAAGGACAG CTTCCGCCCC
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC TCTGCTCCAT
GACATGGACG CCGGTCACCT GAGCTTCGTG GAAGAGGTGT TTGAGAACCA GACCCGGCTT
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG
CTTCCCAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG
GAGCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAAATGG AAGCACTGAA AAGGCACAGG
CAGGCGGAGG CGGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGTCG CATGGAGCCA
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTTGAGG GGGCCCTGGG CGGCGTGATG
GATGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTCGGTGT GAACAGACCC
ACGATTTCCT GCATATTCGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC
CAGACGCTCA TCTTCTACGA GATCGAGATC TTTGGCGAGC CGGCCACAGT TGCTGAGCAA
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ATGGTGCAGA CGAGTTTATG
GGTCGCTGCA TCTGTCAACC GAGTCTGGAA CGGATGCCAC GGCTGGCCTG GTTCCCACTG
ACGAGGGGCA GCCAGCCGTC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCAAG GATCCTGGAT
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGATCCT GGCATGGGGC
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AACATCTCCT CCCCCAGCCT CGTGGTAGAG
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT
GACATCTGCA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC
ATCACCGTCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCC
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA TTGGTGGAGC
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACCTGGA GAAGGATTTT
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA
TCTGTGATTG GTGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCCC AGGGACCCCA GGAGTGCTTG
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGAAAGTGT
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC
CCCTGCACGC TGGAGCCCGT ATTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG
GAGAAGGACC TAAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAGGA CGAAAAGATC
GGTGAGACGG TCGTCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA
CTCCCACAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC
CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT AGAGTCAAGG CACCTGTGTA CCGGACAGAC
CGTGTAATGT TTCAGGATAA AGAATATTCC ATTGAAGAGA TAGAGGCTGG CAGGATCCCA
AACCCACACC TGGGCCCAGT GGAGGAGCGT CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC
CTGGTCCCGG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATCGAG
CAGGGGAAGC TGCAGATGTG GGTCGACCTA TTTCCGAAGG CCCTGGGGCG GCCTGGACCT
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT
ACCAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGT
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCA
GCTGAGCAAG TCTGTACCAT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG
AGCAAAATCC CAGCACGAGT GGTGTTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC
AAGAAGTGCT CCTTGGACCA GCTGGATGAT GCTTTCCACC CAGAATGGTT TGTGTCCCTT
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAG CAGAGAGTGA GCATGAGGAG
CGGCCTGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG
CGCCCCGACA CCTCCTTCCT GTGGTTTACC TCCCCATACA AGACCATGAA GTTCATCCTG
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC
AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28614
Clone ID Related Accession (Same CDS sequence) NM_001130979
Accession Version NM_001130979.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6336bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 12
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, EU515155.1, AI128455.1, DB292971.1 and AK074649.1. Transcript Variant: This variant (12) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (12) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515155.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC
AGAGAGTTCA CAGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAT
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA CATCCATGAG
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCA TCACCTGAGC
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG
AAGCTACAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGCGC CCGGATGCCA
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCAGTGG ATGAGAAGGA GTTCAACCAG
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC TAAGTTGGCC
CTTGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACGGGC
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGACA AGAGTGAAGA TTCCATGTCC
GTCTCCACCT TGAGCTTCGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG
GAACGGATGC CACGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC GTCGGGGGAG
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC
CAGCGTACCG CCATCGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC
CAGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA
AAGTGCGGCT CCTACCTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTGATTG GTGAATTTAA GGGCCTCTTC
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC
CTGCAGCCCA AGGACCCCAA TGGAAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCGTCGACCT GGAGAACAGG
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC
AACCGCATGC CCAAGCCAGC CAAGACAGCC AAGAAGTGCT CCTTGGACCA GCTGGATGAT
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124451.1
CDS377..6712
Misc Feature(1)182..184(+)
Misc Feature(2)434..775(+)
Misc Feature(3)1118..1447(+)
Misc Feature(4)1376..1591(+)
Misc Feature(5)1607..2131(+)
Misc Feature(6)1700..1912(+)
Misc Feature(7)2549..2746(+)
Misc Feature(8)2825..3052(+)
Misc Feature(9)3092..3268(+)
Misc Feature(10)3305..3475(+)
Misc Feature(11)3923..4309(+)
Misc Feature(12)3971..4165(+)
Misc Feature(13)5204..5575(+)
Misc Feature(14)5249..5437(+)
Misc Feature(15)5906..6301(+)
Misc Feature(16)6608..6670(+)
Exon (1)1..464
Gene:DYSF
Gene Synonym:
Exon (2)465..520
Gene:DYSF
Gene Synonym:
Exon (3)521..612
Gene:DYSF
Gene Synonym:
Exon (4)613..718
Gene:DYSF
Gene Synonym:
Exon (5)719..833
Gene:DYSF
Gene Synonym:
Exon (6)834..926
Gene:DYSF
Gene Synonym:
Exon (7)927..1132
Gene:DYSF
Gene Synonym:
Exon (8)1133..1261
Gene:DYSF
Gene Synonym:
Exon (9)1262..1324
Gene:DYSF
Gene Synonym:
Exon (10)1325..1375
Gene:DYSF
Gene Synonym:
Exon (11)1376..1406
Gene:DYSF
Gene Synonym:
Exon (12)1407..1522
Gene:DYSF
Gene Synonym:
Exon (13)1523..1649
Gene:DYSF
Gene Synonym:
Exon (14)1650..1753
Gene:DYSF
Gene Synonym:
Exon (15)1754..1822
Gene:DYSF
Gene Synonym:
Exon (16)1823..1866
Gene:DYSF
Gene Synonym:
Exon (17)1867..1949
Gene:DYSF
Gene Synonym:
Exon (18)1950..1991
Gene:DYSF
Gene Synonym:
Exon (19)1992..2107
Gene:DYSF
Gene Synonym:
Exon (20)2108..2221
Gene:DYSF
Gene Synonym:
Exon (21)2222..2399
Gene:DYSF
Gene Synonym:
Exon (22)2400..2524
Gene:DYSF
Gene Synonym:
Exon (23)2525..2631
Gene:DYSF
Gene Synonym:
Exon (24)2632..2824
Gene:DYSF
Gene Synonym:
Exon (25)2825..2980
Gene:DYSF
Gene Synonym:
Exon (26)2981..3112
Gene:DYSF
Gene Synonym:
Exon (27)3113..3279
Gene:DYSF
Gene Synonym:
Exon (28)3280..3394
Gene:DYSF
Gene Synonym:
Exon (29)3395..3500
Gene:DYSF
Gene Synonym:
Exon (30)3501..3643
Gene:DYSF
Gene Synonym:
Exon (31)3644..3817
Gene:DYSF
Gene Synonym:
Exon (32)3818..3911
Gene:DYSF
Gene Synonym:
Exon (33)3912..3989
Gene:DYSF
Gene Synonym:
Exon (34)3990..4171
Gene:DYSF
Gene Synonym:
Exon (35)4172..4312
Gene:DYSF
Gene Synonym:
Exon (36)4313..4342
Gene:DYSF
Gene Synonym:
Exon (37)4343..4372
Gene:DYSF
Gene Synonym:
Exon (38)4373..4474
Gene:DYSF
Gene Synonym:
Exon (39)4475..4636
Gene:DYSF
Gene Synonym:
Exon (40)4637..4802
Gene:DYSF
Gene Synonym:
Exon (41)4803..4879
Gene:DYSF
Gene Synonym:
Exon (42)4880..4978
Gene:DYSF
Gene Synonym:
Exon (43)4979..5107
Gene:DYSF
Gene Synonym:
Exon (44)5108..5263
Gene:DYSF
Gene Synonym:
Exon (45)5264..5355
Gene:DYSF
Gene Synonym:
Exon (46)5356..5526
Gene:DYSF
Gene Synonym:
Exon (47)5527..5669
Gene:DYSF
Gene Synonym:
Exon (48)5670..5809
Gene:DYSF
Gene Synonym:
Exon (49)5810..5898
Gene:DYSF
Gene Synonym:
Exon (50)5899..5994
Gene:DYSF
Gene Synonym:
Exon (51)5995..6136
Gene:DYSF
Gene Synonym:
Exon (52)6137..6236
Gene:DYSF
Gene Synonym:
Exon (53)6237..6415
Gene:DYSF
Gene Synonym:
Exon (54)6416..6525
Gene:DYSF
Gene Synonym:
Exon (55)6526..6673
Gene:DYSF
Gene Synonym:
Exon (56)6674..6983
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130979
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130979

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA
GGAGAAATAG AAGAGGAGCC TGCAGGTGCT GTCAAGCCTT CGAAAGCCTC AGACTTGGAT
GACTACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC
AGAGAGTTCA CAGGCTTCCC AGACCCCTAC ACAGAGCTCA ACACAGGCAA GGGGGAAGGT
GTGGCTTATC GTGGCCGGCT TCTGCTCTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAA
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCAGCCACCA TGCTGCAGGA TGTGGATGAT
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TACGGGAACA AGTTCGACAT GACCTGCCTG
CCGCTGGCCT CCACCACTCA GTACAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC
CTACCCTGGG GTAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCGCTGGTG
GCTCAGCTGA CGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA CATCCATGAG
ACACCCTCTG CCACCCACCT GGACCAGTAC CTGTACCAGC TGCGCACCCA TCACCTGAGC
CAAATCACTG AGGCTGCCCT GGCCCTGAAG CTCGGCCACA GTGAGCTCCC TGCAGCTCTG
GAGCAGGCGG AGGACTGGCT CCTGCGTCTG CGTGCCCTGG CAGAGGAGCC CCAGAACAGC
CTGCCGGACA TCGTCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG
AAGCTACAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGCGC CCGGATGCCA
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCAGTGG ATGAGAAGGA GTTCAACCAG
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACGAGAC TAAGTTGGCC
CTTGTTGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACGGGC
AAGATCAAGC TACCCAAGGA CAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT
TGGTTCGTGT GTCCGGAGAA GACTCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTC
GTGGAAGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AGGATGACAT TGAGTGCCCA
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT
CTCAGCCAAA TGGAAGCACT GAAAAGGCAC AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC
CGCCGCCGCC GCTGGCGCCG TCGCATGGAG CCACTGGAGA AGACGGGGCC TGCAGCTGTG
TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG ATGGATGACA AGAGTGAAGA TTCCATGTCC
GTCTCCACCT TGAGCTTCGG TGTGAACAGA CCCACGATTT CCTGCATATT CGACTATGGG
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG
ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG CAACCGCCCA GCATTGTGGT GGAGCTGTAC
GACCATGACA CTTATGGTGC AGACGAGTTT ATGGGTCGCT GCATCTGTCA ACCGAGTCTG
GAACGGATGC CACGGCTGGC CTGGTTCCCA CTGACGAGGG GCAGCCAGCC GTCGGGGGAG
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT
TTTGAGGTGC AGGAGACATC AAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC
CAGCGTACCG CCATCGAGAT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG
GCCAACATCT CCTCCCCCAG CCTCGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GCACCCTCTT CATGGAAGTG
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCG TCAAGGTCAT CGATAACCGC
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC
CAGGAGGAAG AGTTCATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA
AAGTGCGGCT CCTACCTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTGATTG GTGAATTTAA GGGCCTCTTC
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG
CTGGCCGCCC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC
CTGCAGCCCA AGGACCCCAA TGGAAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGC TGGAGCCCGT ATTTGGAAAG
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TAAAGATCAC TCTCTATGAC
TATGACCTCC TCTCCAAGGA CGAAAAGATC GGTGAGACGG TCGTCGACCT GGAGAACAGG
CTGCTGTCCA AGTTTGGGGC TCGCTGTGGA CTCCCACAGA CCTACTGTGT CTCTGGACCG
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCAT
AGAGTCAAGG CACCTGTGTA CCGGACAGAC CGTGTAATGT TTCAGGATAA AGAATATTCC
ATTGAAGAGA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCAGT GGAGGAGCGT
CTGGCTCTGC ATGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCACGTGGA GTCACGGCCC
CTCTACAGCC CCCTGCAGCC AGACATCGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTA
TTTCCGAAGG CCCTGGGGCG GCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACCAGAGATG TGATCCTGGA TGACCTGAGC
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA
CACAAGCAAA AGACAGACGT GCATTATCGT TCCCTGGGAG GTGAAGGCAA CTTCAACTGG
AGGTTCATTT TCCCCTTCGA CTACCTGCCA GCTGAGCAAG TCTGTACCAT TGCCAAGAAG
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTGTTCCAG
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC
AACCGCATGC CCAAGCCAGC CAAGACAGCC AAGAAGTGCT CCTTGGACCA GCTGGATGAT
GCTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG
CCCTGTGTAG CAGAAGAGGG TGAGAAGAAA ATACTGGCGG GCAAGCTGGA AATGACCTTG
GAGATTGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAGCCC
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTTACC
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28604
Clone ID Related Accession (Same CDS sequence) NM_001130980
Accession Version NM_001130980.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6294bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 13
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, EU515158.1, AI128455.1, DB292971.1 and AK074649.1. Transcript Variant: This variant (13) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (13) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC
AACCTCTATG GCAGTCCCAG AGAGTTCACA GGCTTCCCAG ACCCCTACAC AGAGCTCAAC
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG
CTGCAGGATG TGGATGATGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAC
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT
CTGGGTGACA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG
CGCACCCATC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC
TGTGGCAAGA ATTGTGGGAA GCTACAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG
CCTGGCGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG
AACGAGACTA AGTTGGCCCT TGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG
TTTTCTGACG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGACAAG
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTCGGTG TGAACAGACC CACGATTTCC
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC
ATCTGTCAAC CGAGTCTGGA ACGGATGCCA CGGCTGGCCT GGTTCCCACT GACGAGGGGC
AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACCTGGAGA AGGATTTTGA CACCCTGAAG
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTGATTGGT
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GAAAGTGTGA TCCTTACATC
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC
GTCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCAA GAAGTGCTCC
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124452.1
CDS377..6670
Misc Feature(1)182..184(+)
Misc Feature(2)434..775(+)
Misc Feature(3)1118..1447(+)
Misc Feature(4)1376..1591(+)
Misc Feature(5)1607..2089(+)
Misc Feature(6)1700..1912(+)
Misc Feature(7)2507..2704(+)
Misc Feature(8)2783..3010(+)
Misc Feature(9)3050..3226(+)
Misc Feature(10)3263..3433(+)
Misc Feature(11)3881..4267(+)
Misc Feature(12)3929..4123(+)
Misc Feature(13)5162..5533(+)
Misc Feature(14)5207..5395(+)
Misc Feature(15)5864..6259(+)
Exon (1)1..464
Gene:DYSF
Gene Synonym:
Exon (2)465..520
Gene:DYSF
Gene Synonym:
Exon (3)521..612
Gene:DYSF
Gene Synonym:
Exon (4)613..718
Gene:DYSF
Gene Synonym:
Exon (5)719..833
Gene:DYSF
Gene Synonym:
Exon (6)834..926
Gene:DYSF
Gene Synonym:
Exon (7)927..1132
Gene:DYSF
Gene Synonym:
Exon (8)1133..1261
Gene:DYSF
Gene Synonym:
Exon (9)1262..1324
Gene:DYSF
Gene Synonym:
Exon (10)1325..1375
Gene:DYSF
Gene Synonym:
Exon (11)1376..1406
Gene:DYSF
Gene Synonym:
Exon (12)1407..1522
Gene:DYSF
Gene Synonym:
Exon (13)1523..1649
Gene:DYSF
Gene Synonym:
Exon (14)1650..1753
Gene:DYSF
Gene Synonym:
Exon (15)1754..1822
Gene:DYSF
Gene Synonym:
Exon (16)1823..1866
Gene:DYSF
Gene Synonym:
Exon (17)1867..1949
Gene:DYSF
Gene Synonym:
Exon (18)1950..2065
Gene:DYSF
Gene Synonym:
Exon (19)2066..2179
Gene:DYSF
Gene Synonym:
Exon (20)2180..2357
Gene:DYSF
Gene Synonym:
Exon (21)2358..2482
Gene:DYSF
Gene Synonym:
Exon (22)2483..2589
Gene:DYSF
Gene Synonym:
Exon (23)2590..2782
Gene:DYSF
Gene Synonym:
Exon (24)2783..2938
Gene:DYSF
Gene Synonym:
Exon (25)2939..3070
Gene:DYSF
Gene Synonym:
Exon (26)3071..3237
Gene:DYSF
Gene Synonym:
Exon (27)3238..3352
Gene:DYSF
Gene Synonym:
Exon (28)3353..3458
Gene:DYSF
Gene Synonym:
Exon (29)3459..3601
Gene:DYSF
Gene Synonym:
Exon (30)3602..3775
Gene:DYSF
Gene Synonym:
Exon (31)3776..3869
Gene:DYSF
Gene Synonym:
Exon (32)3870..3947
Gene:DYSF
Gene Synonym:
Exon (33)3948..4129
Gene:DYSF
Gene Synonym:
Exon (34)4130..4270
Gene:DYSF
Gene Synonym:
Exon (35)4271..4300
Gene:DYSF
Gene Synonym:
Exon (36)4301..4330
Gene:DYSF
Gene Synonym:
Exon (37)4331..4432
Gene:DYSF
Gene Synonym:
Exon (38)4433..4594
Gene:DYSF
Gene Synonym:
Exon (39)4595..4760
Gene:DYSF
Gene Synonym:
Exon (40)4761..4837
Gene:DYSF
Gene Synonym:
Exon (41)4838..4936
Gene:DYSF
Gene Synonym:
Exon (42)4937..5065
Gene:DYSF
Gene Synonym:
Exon (43)5066..5221
Gene:DYSF
Gene Synonym:
Exon (44)5222..5313
Gene:DYSF
Gene Synonym:
Exon (45)5314..5484
Gene:DYSF
Gene Synonym:
Exon (46)5485..5627
Gene:DYSF
Gene Synonym:
Exon (47)5628..5767
Gene:DYSF
Gene Synonym:
Exon (48)5768..5856
Gene:DYSF
Gene Synonym:
Exon (49)5857..5952
Gene:DYSF
Gene Synonym:
Exon (50)5953..6094
Gene:DYSF
Gene Synonym:
Exon (51)6095..6194
Gene:DYSF
Gene Synonym:
Exon (52)6195..6373
Gene:DYSF
Gene Synonym:
Exon (53)6374..6483
Gene:DYSF
Gene Synonym:
Exon (54)6484..6631
Gene:DYSF
Gene Synonym:
Exon (55)6632..6941
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130980
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130980

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC
AACCTCTATG GCAGTCCCAG AGAGTTCACA GGCTTCCCAG ACCCCTACAC AGAGCTCAAC
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG
CTGCAGGATG TGGATGATGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAC
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT
CTGGGTGACA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG
CGCACCCATC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC
TGTGGCAAGA ATTGTGGGAA GCTACAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG
CCTGGCGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG
AACGAGACTA AGTTGGCCCT TGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG
TTTTCTGACG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGACAAG
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTCGGTG TGAACAGACC CACGATTTCC
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC
ATCTGTCAAC CGAGTCTGGA ACGGATGCCA CGGCTGGCCT GGTTCCCACT GACGAGGGGC
AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTCATCGATT GGTGGAGCAA ATTCTTTGCC
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACCTGGAGA AGGATTTTGA CACCCTGAAG
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTGATTGGT
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC
CCAAGACAGT TCCACCAGCT GGCCGCCCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GAAAGTGTGA TCCTTACATC
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGCTG
GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTA
AAGATCACTC TCTATGACTA TGACCTCCTC TCCAAGGACG AAAAGATCGG TGAGACGGTC
GTCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGGGGCTC GCTGTGGACT CCCACAGACC
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC
CTCTTCTGCC AGCAGCATAG AGTCAAGGCA CCTGTGTACC GGACAGACCG TGTAATGTTT
CAGGATAAAG AATATTCCAT TGAAGAGATA GAGGCTGGCA GGATCCCAAA CCCACACCTG
GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATCGAGCA GGGGAAGCTG
CAGATGTGGG TCGACCTATT TCCGAAGGCC CTGGGGCGGC CTGGACCTCC CTTCAACATC
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC CAGAGATGTG
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGTTC CCTGGGAGGT
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCAGC TGAGCAAGTC
TGTACCATTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA
GCACGAGTGG TGTTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCAA GAAGTGCTCC
TTGGACCAGC TGGATGATGC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA
ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC
AAGCTGGAAA TGACCTTGGA GATTGTAGCA GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC
TCCTTCCTGT GGTTTACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28599
Clone ID Related Accession (Same CDS sequence) NM_001130981
Accession Version NM_001130981.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 14
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI192657.1, EU515160.1, AI128455.1, DB292971.1 and AK074649.1. Transcript Variant: This variant (14) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at an upstream AUG and a shorter isoform (14) with an alternate N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515160.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC
AACCTCTATG GCAGTCCCAG AGAGTTCACA GGCTTCCCAG ACCCCTACAC AGAGCTCAAC
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG
CTGCAGGATG TGGATGATGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAC
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT
CTGGGTGACA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG
CGCACCCATC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC
TGTGGCAAGA ATTGTGGGAA GCTACAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG
CCTGGCGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG
AACGAGACTA AGTTGGCCCT TGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG
TTTTCTGACG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGACAAG
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTCGGTG TGAACAGACC CACGATTTCC
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC
ATCTGTCAAC CGAGTCTGGA ACGGATGCCA CGGCTGGCCT GGTTCCCACT GACGAGGGGC
AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC CACTAACACG
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACCTGG AGAAGGATTT TGACACCCTG
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTGATT
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGAAAGTG TGATCCTTAC
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG
GTCGTCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CAAGAAGTGC
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124453.1
CDS377..6733
Misc Feature(1)182..184(+)
Misc Feature(2)434..775(+)
Misc Feature(3)1118..1447(+)
Misc Feature(4)1376..1591(+)
Misc Feature(5)1607..2089(+)
Misc Feature(6)1700..1912(+)
Misc Feature(7)2507..2704(+)
Misc Feature(8)2783..3010(+)
Misc Feature(9)3050..3226(+)
Misc Feature(10)3263..3433(+)
Misc Feature(11)3881..4267(+)
Misc Feature(12)3929..4123(+)
Misc Feature(13)5225..5596(+)
Misc Feature(14)5270..5458(+)
Misc Feature(15)5927..6322(+)
Exon (1)1..464
Gene:DYSF
Gene Synonym:
Exon (2)465..520
Gene:DYSF
Gene Synonym:
Exon (3)521..612
Gene:DYSF
Gene Synonym:
Exon (4)613..718
Gene:DYSF
Gene Synonym:
Exon (5)719..833
Gene:DYSF
Gene Synonym:
Exon (6)834..926
Gene:DYSF
Gene Synonym:
Exon (7)927..1132
Gene:DYSF
Gene Synonym:
Exon (8)1133..1261
Gene:DYSF
Gene Synonym:
Exon (9)1262..1324
Gene:DYSF
Gene Synonym:
Exon (10)1325..1375
Gene:DYSF
Gene Synonym:
Exon (11)1376..1406
Gene:DYSF
Gene Synonym:
Exon (12)1407..1522
Gene:DYSF
Gene Synonym:
Exon (13)1523..1649
Gene:DYSF
Gene Synonym:
Exon (14)1650..1753
Gene:DYSF
Gene Synonym:
Exon (15)1754..1822
Gene:DYSF
Gene Synonym:
Exon (16)1823..1866
Gene:DYSF
Gene Synonym:
Exon (17)1867..1949
Gene:DYSF
Gene Synonym:
Exon (18)1950..2065
Gene:DYSF
Gene Synonym:
Exon (19)2066..2179
Gene:DYSF
Gene Synonym:
Exon (20)2180..2357
Gene:DYSF
Gene Synonym:
Exon (21)2358..2482
Gene:DYSF
Gene Synonym:
Exon (22)2483..2589
Gene:DYSF
Gene Synonym:
Exon (23)2590..2782
Gene:DYSF
Gene Synonym:
Exon (24)2783..2938
Gene:DYSF
Gene Synonym:
Exon (25)2939..3070
Gene:DYSF
Gene Synonym:
Exon (26)3071..3237
Gene:DYSF
Gene Synonym:
Exon (27)3238..3352
Gene:DYSF
Gene Synonym:
Exon (28)3353..3458
Gene:DYSF
Gene Synonym:
Exon (29)3459..3601
Gene:DYSF
Gene Synonym:
Exon (30)3602..3775
Gene:DYSF
Gene Synonym:
Exon (31)3776..3869
Gene:DYSF
Gene Synonym:
Exon (32)3870..3947
Gene:DYSF
Gene Synonym:
Exon (33)3948..4129
Gene:DYSF
Gene Synonym:
Exon (34)4130..4270
Gene:DYSF
Gene Synonym:
Exon (35)4271..4300
Gene:DYSF
Gene Synonym:
Exon (36)4301..4330
Gene:DYSF
Gene Synonym:
Exon (37)4331..4432
Gene:DYSF
Gene Synonym:
Exon (38)4433..4594
Gene:DYSF
Gene Synonym:
Exon (39)4595..4760
Gene:DYSF
Gene Synonym:
Exon (40)4761..4837
Gene:DYSF
Gene Synonym:
Exon (41)4838..4900
Gene:DYSF
Gene Synonym:
Exon (42)4901..4999
Gene:DYSF
Gene Synonym:
Exon (43)5000..5128
Gene:DYSF
Gene Synonym:
Exon (44)5129..5284
Gene:DYSF
Gene Synonym:
Exon (45)5285..5376
Gene:DYSF
Gene Synonym:
Exon (46)5377..5547
Gene:DYSF
Gene Synonym:
Exon (47)5548..5690
Gene:DYSF
Gene Synonym:
Exon (48)5691..5830
Gene:DYSF
Gene Synonym:
Exon (49)5831..5919
Gene:DYSF
Gene Synonym:
Exon (50)5920..6015
Gene:DYSF
Gene Synonym:
Exon (51)6016..6157
Gene:DYSF
Gene Synonym:
Exon (52)6158..6257
Gene:DYSF
Gene Synonym:
Exon (53)6258..6436
Gene:DYSF
Gene Synonym:
Exon (54)6437..6546
Gene:DYSF
Gene Synonym:
Exon (55)6547..6694
Gene:DYSF
Gene Synonym:
Exon (56)6695..7004
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130981
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130981

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
ATGCTGAGGG TCTTCATCCT CTATGCCGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC
AGCGTGAACC CTGTATGGAA TGAGGGATTT GAATGGGACC TCAAGGGCAT CCCCCTGGAC
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG
CCCTCCCCGA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG
ACTTGGTCCC TGCTCAGTGA CAGCACCATG GACACGAGAT ACTCTGGAAA GAAGTGGCCG
GCCCCCACGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG
GCGGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA
CCTTCACGTC CTCCGCCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC GCCTACATCT
AGAAAGCTGC TGTCAGACAA ACCGCAGGAT TTCCAGATCA GGGTCCAGGT GATCGAGGGG
CGCCAGCTGC CGGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACCGCTGC AGGGCAGACC
AAGCGGACGC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC
TTGTTTGACT CTCCTGGGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCT
CGTTCTCTCA GGACAGATGC TCTCCTCGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC
TCTGCTGGGG CCAGAGGCTA CCTGAAAACA AGCCTTTGTG TGCTGGGGCC TGGGGACGAA
GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC
CGGCCCACAG GCGTAGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG
GACTTGCCGC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTCGAG
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTG
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT
GCCATGTTTC CCTCCATGTG CGAAAAAATG AGGATTCGTA TCATAGACTG GGACCGCCTG
ACTCACAATG ACATCGTGGC TACCACCTAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA
GGAGAAATAG AAGTGGATGA CTACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC
AACCTCTATG GCAGTCCCAG AGAGTTCACA GGCTTCCCAG ACCCCTACAC AGAGCTCAAC
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTCTCCCT GGAGACCAAG
CTGGTGGAGC ACAGTGAACA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC AGCCACCATG
CTGCAGGATG TGGATGATGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA CGGGAACAAG
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ACAGCCGTGC AGTCTTTGAC
GGGTGCCACT ACTACTACCT ACCCTGGGGT AACGTGAAAC CTGTGGTGGT GCTGTCATCC
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG
GACGTGGACT CGCTGGTGGC TCAGCTGACG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT
CTGGGTGACA TCCATGAGAC ACCCTCTGCC ACCCACCTGG ACCAGTACCT GTACCAGCTG
CGCACCCATC ACCTGAGCCA AATCACTGAG GCTGCCCTGG CCCTGAAGCT CGGCCACAGT
GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG GACTGGCTCC TGCGTCTGCG TGCCCTGGCA
GAGGAGCCCC AGAACAGCCT GCCGGACATC GTCATCTGGA TGCTGCAGGG AGACAAGCGT
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC
TGTGGCAAGA ATTGTGGGAA GCTACAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG
CCTGGCGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG
AACGAGACTA AGTTGGCCCT TGTTGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG
TTTTCTGACG TCACGGGCAA GATCAAGCTA CCCAAGGACA GCTTCCGCCC CTCGGCCGGC
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CTCTGCTCCA TGACATGGAC
GCCGGTCACC TGAGCTTCGT GGAAGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAG
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGAGCGGAAG
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG
CGCCTGCGCA GGAGGGATCT CAGCCAAATG GAAGCACTGA AAAGGCACAG GCAGGCGGAG
GCGGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGTC GCATGGAGCC ACTGGAGAAG
ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG GGGGCCCTGG GCGGCGTGAT GGATGACAAG
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTCGGTG TGAACAGACC CACGATTTCC
TGCATATTCG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC
ATCTTCTACG AGATCGAGAT CTTTGGCGAG CCGGCCACAG TTGCTGAGCA ACCGCCCAGC
ATTGTGGTGG AGCTGTACGA CCATGACACT TATGGTGCAG ACGAGTTTAT GGGTCGCTGC
ATCTGTCAAC CGAGTCTGGA ACGGATGCCA CGGCTGGCCT GGTTCCCACT GACGAGGGGC
AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCAA GGATCCTGGA TGAGTCTGAG
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGATCC TGGCATGGGG CCTGCGGAAC
ATGAAGAGTT ACCAGCTGGC CAACATCTCC TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGC
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCGTC
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC CACTAACACG
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACCTGG AGAAGGATTT TGACACCCTG
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTGATT
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGAAAGTG TGATCCTTAC
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG
GTCGTCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CAAGAAGTGC
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28609
Clone ID Related Accession (Same CDS sequence) NM_001130982
Accession Version NM_001130982.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6339bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 7
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU515161.1, AF075575.1 and DB292971.1. Transcript Variant: This variant (7) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 7 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT
CCCAGAGAGT TCACAGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT
GATGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGACATCCAT
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCATCACCTG
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT
GGGAAGCTAC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG CGCCCGGATG
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCAG TGGATGAGAA GGAGTTCAAC
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACTAAGTTG
GCCCTTGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACG
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG ACAAGAGTGA AGATTCCATG
TCCGTCTCCA CCTTGAGCTT CGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT
CTGGAACGGA TGCCACGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCGTCGGGG
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG
CTCCAGCGTA CCGCCATCGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTG GGGGCCAGAC GGTGCAGTCC
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC
ATCCAGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG
GAAAAGTGCG GCTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTGA TTGGTGAATT TAAGGGCCTC
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT
GGCCTGCAGC CCAAGGACCC CAATGGAAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124454.1
CDS142..6480
Misc Feature(1)154..543(+)
Misc Feature(2)886..1215(+)
Misc Feature(3)1144..1359(+)
Misc Feature(4)1375..1899(+)
Misc Feature(5)1468..1680(+)
Misc Feature(6)2317..2514(+)
Misc Feature(7)2593..2820(+)
Misc Feature(8)2860..3036(+)
Misc Feature(9)3073..3243(+)
Misc Feature(10)3691..4077(+)
Misc Feature(11)3739..3933(+)
Misc Feature(12)4972..5343(+)
Misc Feature(13)5017..5205(+)
Misc Feature(14)5674..6069(+)
Exon (1)1..232
Gene:DYSF
Gene Synonym:
Exon (2)233..288
Gene:DYSF
Gene Synonym:
Exon (3)289..380
Gene:DYSF
Gene Synonym:
Exon (4)381..486
Gene:DYSF
Gene Synonym:
Exon (5)487..601
Gene:DYSF
Gene Synonym:
Exon (6)602..694
Gene:DYSF
Gene Synonym:
Exon (7)695..900
Gene:DYSF
Gene Synonym:
Exon (8)901..1029
Gene:DYSF
Gene Synonym:
Exon (9)1030..1092
Gene:DYSF
Gene Synonym:
Exon (10)1093..1143
Gene:DYSF
Gene Synonym:
Exon (11)1144..1174
Gene:DYSF
Gene Synonym:
Exon (12)1175..1290
Gene:DYSF
Gene Synonym:
Exon (13)1291..1417
Gene:DYSF
Gene Synonym:
Exon (14)1418..1521
Gene:DYSF
Gene Synonym:
Exon (15)1522..1590
Gene:DYSF
Gene Synonym:
Exon (16)1591..1634
Gene:DYSF
Gene Synonym:
Exon (17)1635..1717
Gene:DYSF
Gene Synonym:
Exon (18)1718..1759
Gene:DYSF
Gene Synonym:
Exon (19)1760..1875
Gene:DYSF
Gene Synonym:
Exon (20)1876..1989
Gene:DYSF
Gene Synonym:
Exon (21)1990..2167
Gene:DYSF
Gene Synonym:
Exon (22)2168..2292
Gene:DYSF
Gene Synonym:
Exon (23)2293..2399
Gene:DYSF
Gene Synonym:
Exon (24)2400..2592
Gene:DYSF
Gene Synonym:
Exon (25)2593..2748
Gene:DYSF
Gene Synonym:
Exon (26)2749..2880
Gene:DYSF
Gene Synonym:
Exon (27)2881..3047
Gene:DYSF
Gene Synonym:
Exon (28)3048..3162
Gene:DYSF
Gene Synonym:
Exon (29)3163..3268
Gene:DYSF
Gene Synonym:
Exon (30)3269..3411
Gene:DYSF
Gene Synonym:
Exon (31)3412..3585
Gene:DYSF
Gene Synonym:
Exon (32)3586..3679
Gene:DYSF
Gene Synonym:
Exon (33)3680..3757
Gene:DYSF
Gene Synonym:
Exon (34)3758..3939
Gene:DYSF
Gene Synonym:
Exon (35)3940..4080
Gene:DYSF
Gene Synonym:
Exon (36)4081..4110
Gene:DYSF
Gene Synonym:
Exon (37)4111..4140
Gene:DYSF
Gene Synonym:
Exon (38)4141..4242
Gene:DYSF
Gene Synonym:
Exon (39)4243..4404
Gene:DYSF
Gene Synonym:
Exon (40)4405..4570
Gene:DYSF
Gene Synonym:
Exon (41)4571..4647
Gene:DYSF
Gene Synonym:
Exon (42)4648..4746
Gene:DYSF
Gene Synonym:
Exon (43)4747..4875
Gene:DYSF
Gene Synonym:
Exon (44)4876..5031
Gene:DYSF
Gene Synonym:
Exon (45)5032..5123
Gene:DYSF
Gene Synonym:
Exon (46)5124..5294
Gene:DYSF
Gene Synonym:
Exon (47)5295..5437
Gene:DYSF
Gene Synonym:
Exon (48)5438..5577
Gene:DYSF
Gene Synonym:
Exon (49)5578..5666
Gene:DYSF
Gene Synonym:
Exon (50)5667..5762
Gene:DYSF
Gene Synonym:
Exon (51)5763..5904
Gene:DYSF
Gene Synonym:
Exon (52)5905..6004
Gene:DYSF
Gene Synonym:
Exon (53)6005..6183
Gene:DYSF
Gene Synonym:
Exon (54)6184..6293
Gene:DYSF
Gene Synonym:
Exon (55)6294..6441
Gene:DYSF
Gene Synonym:
Exon (56)6442..6751
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130982
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130982

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GCTGTCAAGC CTTCGAAAGC CTCAGACTTG
GATGACTACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT
CCCAGAGAGT TCACAGGCTT CCCAGACCCC TACACAGAGC TCAACACAGG CAAGGGGGAA
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGT
GAACAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCAGCCA CCATGCTGCA GGATGTGGAT
GATGCCATCC AGTTTGAGGT CAGCATCGGG AACTACGGGA ACAAGTTCGA CATGACCTGC
CTGCCGCTGG CCTCCACCAC TCAGTACAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC
TACCTACCCT GGGGTAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCGCTG
GTGGCTCAGC TGACGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGACATCCAT
GAGACACCCT CTGCCACCCA CCTGGACCAG TACCTGTACC AGCTGCGCAC CCATCACCTG
AGCCAAATCA CTGAGGCTGC CCTGGCCCTG AAGCTCGGCC ACAGTGAGCT CCCTGCAGCT
CTGGAGCAGG CGGAGGACTG GCTCCTGCGT CTGCGTGCCC TGGCAGAGGA GCCCCAGAAC
AGCCTGCCGG ACATCGTCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT
GGGAAGCTAC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG CGCCCGGATG
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCAG TGGATGAGAA GGAGTTCAAC
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACGA GACTAAGTTG
GCCCTTGTTG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACG
GGCAAGATCA AGCTACCCAA GGACAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA
GATTGGTTCG TGTGTCCGGA GAAGACTCTG CTCCATGACA TGGACGCCGG TCACCTGAGC
TTCGTGGAAG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAGGATGA CATTGAGTGC
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGAGC GGAAGCCGAA GCACTGGGTC
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG
GATCTCAGCC AAATGGAAGC ACTGAAAAGG CACAGGCAGG CGGAGGCGGA GGGCGAGGGC
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC
TTCCGCCGCC GCCGCTGGCG CCGTCGCATG GAGCCACTGG AGAAGACGGG GCCTGCAGCT
GTGTTTGCCC TTGAGGGGGC CCTGGGCGGC GTGATGGATG ACAAGAGTGA AGATTCCATG
TCCGTCTCCA CCTTGAGCTT CGGTGTGAAC AGACCCACGA TTTCCTGCAT ATTCGACTAT
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC
GAGATCTTTG GCGAGCCGGC CACAGTTGCT GAGCAACCGC CCAGCATTGT GGTGGAGCTG
TACGACCATG ACACTTATGG TGCAGACGAG TTTATGGGTC GCTGCATCTG TCAACCGAGT
CTGGAACGGA TGCCACGGCT GGCCTGGTTC CCACTGACGA GGGGCAGCCA GCCGTCGGGG
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT
GGTTTTGAGG TGCAGGAGAC ATCAAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG
CTCCAGCGTA CCGCCATCGA GATCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG
CTGGCCAACA TCTCCTCCCC CAGCCTCGTG GTAGAGTGTG GGGGCCAGAC GGTGCAGTCC
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGCACCCT CTTCATGGAA
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCGTCAAGGT CATCGATAAC
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC
ATCCAGGAGG AAGAGTTCAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG
GAAAAGTGCG GCTCCTACCT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTGA TTGGTGAATT TAAGGGCCTC
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC
CAGCTGGCCG CCCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT
GGCCTGCAGC CCAAGGACCC CAATGGAAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGCTGGAGCC CGTATTTGGA
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTAAAGAT CACTCTCTAT
GACTATGACC TCCTCTCCAA GGACGAAAAG ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG
CATAGAGTCA AGGCACCTGT GTACCGGACA GACCGTGTAA TGTTTCAGGA TAAAGAATAT
TCCATTGAAG AGATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC AGTGGAGGAG
CGTCTGGCTC TGCATGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCACGT GGAGTCACGG
CCCCTCTACA GCCCCCTGCA GCCAGACATC GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC
CTATTTCCGA AGGCCCTGGG GCGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACCAGAG ATGTGATCCT GGATGACCTG
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA
GAACACAAGC AAAAGACAGA CGTGCATTAT CGTTCCCTGG GAGGTGAAGG CAACTTCAAC
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCAGCTGAGC AAGTCTGTAC CATTGCCAAG
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTGTTC
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT
GATGCTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG
TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG AAAATACTGG CGGGCAAGCT GGAAATGACC
TTGGAGATTG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAG
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTT
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28616
Clone ID Related Accession (Same CDS sequence) NM_001130983
Accession Version NM_001130983.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6309bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU515163.1, AF075575.1, DB292971.1, EU515157.1 and AK074649.1. Transcript Variant: This variant (6) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 6 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515163.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CAGGCTTCCC AGACCCCTAC
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC
TCAGCCACCA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TTGAGGTCAG CATCGGGAAC
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC
TGCAGCCAGC CTCTGGGTGA CATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC
CTGTACCAGC TGCGCACCCA TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTACAGA CAATCTTTCT GAAATATCCG
ATGGAGAAGG TGCCTGGCGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG
CTCTCAGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT
GAAACCTATG AGAACGAGAC TAAGTTGGCC CTTGTTGGGA ACTGGGGCAC AACGGGCCTC
ACCTACCCCA AGTTTTCTGA CGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG
ATGGATGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTCGG TGTGAACAGA
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CACGGCTGGC CTGGTTCCCA
CTGACGAGGG GCAGCCAGCC GTCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGAT CCTGGCATGG
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC
CCCACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGATTGGTGG
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACCT GGAGAAGGAT
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT
CCATCTGTGA TTGGTGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCG CCCAGGGACC CCAGGAGTGC
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGAAAG
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
ATCCCCTGCA CGCTGGAGCC CGTATTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT
CTGGAGAAGG ACCTAAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA GGACGAAAAG
ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CATAGAGTCA AGGCACCTGT GTACCGGACA
GACCGTGTAA TGTTTCAGGA TAAAGAATAT TCCATTGAAG AGATAGAGGC TGGCAGGATC
CCAAACCCAC ACCTGGGCCC AGTGGAGGAG CGTCTGGCTC TGCATGTGCT TCAGCAGCAG
GGCCTGGTCC CGGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATC
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTATTTCCGA AGGCCCTGGG GCGGCCTGGA
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT
CGTTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG
CCAGCTGAGC AAGTCTGTAC CATTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT
GAGAGCAAAA TCCCAGCACG AGTGGTGTTC CAGATCTGGG ACAATGACAA GTTCTCCTTT
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA
GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT GATGCTTTCC ACCCAGAATG GTTTGTGTCC
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG
AAAATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAGCAGAGAG TGAGCATGAG
GAGCGGCCTG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA
AGGCGCCCCG ACACCTCCTT CCTGTGGTTT ACCTCCCCAT ACAAGACCAT GAAGTTCATC
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124455.1
CDS142..6450
Misc Feature(1)154..543(+)
Misc Feature(2)793..1122(+)
Misc Feature(3)1051..1266(+)
Misc Feature(4)1282..1806(+)
Misc Feature(5)1375..1587(+)
Misc Feature(6)2224..2421(+)
Misc Feature(7)2500..2727(+)
Misc Feature(8)2767..2943(+)
Misc Feature(9)2980..3150(+)
Misc Feature(10)3598..3984(+)
Misc Feature(11)3646..3840(+)
Misc Feature(12)4942..5313(+)
Misc Feature(13)4987..5175(+)
Misc Feature(14)5644..6039(+)
Misc Feature(15)6346..6408(+)
Exon (1)1..232
Gene:DYSF
Gene Synonym:
Exon (2)233..288
Gene:DYSF
Gene Synonym:
Exon (3)289..380
Gene:DYSF
Gene Synonym:
Exon (4)381..486
Gene:DYSF
Gene Synonym:
Exon (5)487..601
Gene:DYSF
Gene Synonym:
Exon (6)602..807
Gene:DYSF
Gene Synonym:
Exon (7)808..936
Gene:DYSF
Gene Synonym:
Exon (8)937..999
Gene:DYSF
Gene Synonym:
Exon (9)1000..1050
Gene:DYSF
Gene Synonym:
Exon (10)1051..1081
Gene:DYSF
Gene Synonym:
Exon (11)1082..1197
Gene:DYSF
Gene Synonym:
Exon (12)1198..1324
Gene:DYSF
Gene Synonym:
Exon (13)1325..1428
Gene:DYSF
Gene Synonym:
Exon (14)1429..1497
Gene:DYSF
Gene Synonym:
Exon (15)1498..1541
Gene:DYSF
Gene Synonym:
Exon (16)1542..1624
Gene:DYSF
Gene Synonym:
Exon (17)1625..1666
Gene:DYSF
Gene Synonym:
Exon (18)1667..1782
Gene:DYSF
Gene Synonym:
Exon (19)1783..1896
Gene:DYSF
Gene Synonym:
Exon (20)1897..2074
Gene:DYSF
Gene Synonym:
Exon (21)2075..2199
Gene:DYSF
Gene Synonym:
Exon (22)2200..2306
Gene:DYSF
Gene Synonym:
Exon (23)2307..2499
Gene:DYSF
Gene Synonym:
Exon (24)2500..2655
Gene:DYSF
Gene Synonym:
Exon (25)2656..2787
Gene:DYSF
Gene Synonym:
Exon (26)2788..2954
Gene:DYSF
Gene Synonym:
Exon (27)2955..3069
Gene:DYSF
Gene Synonym:
Exon (28)3070..3175
Gene:DYSF
Gene Synonym:
Exon (29)3176..3318
Gene:DYSF
Gene Synonym:
Exon (30)3319..3492
Gene:DYSF
Gene Synonym:
Exon (31)3493..3586
Gene:DYSF
Gene Synonym:
Exon (32)3587..3664
Gene:DYSF
Gene Synonym:
Exon (33)3665..3846
Gene:DYSF
Gene Synonym:
Exon (34)3847..3987
Gene:DYSF
Gene Synonym:
Exon (35)3988..4017
Gene:DYSF
Gene Synonym:
Exon (36)4018..4047
Gene:DYSF
Gene Synonym:
Exon (37)4048..4149
Gene:DYSF
Gene Synonym:
Exon (38)4150..4311
Gene:DYSF
Gene Synonym:
Exon (39)4312..4477
Gene:DYSF
Gene Synonym:
Exon (40)4478..4554
Gene:DYSF
Gene Synonym:
Exon (41)4555..4617
Gene:DYSF
Gene Synonym:
Exon (42)4618..4716
Gene:DYSF
Gene Synonym:
Exon (43)4717..4845
Gene:DYSF
Gene Synonym:
Exon (44)4846..5001
Gene:DYSF
Gene Synonym:
Exon (45)5002..5093
Gene:DYSF
Gene Synonym:
Exon (46)5094..5264
Gene:DYSF
Gene Synonym:
Exon (47)5265..5407
Gene:DYSF
Gene Synonym:
Exon (48)5408..5547
Gene:DYSF
Gene Synonym:
Exon (49)5548..5636
Gene:DYSF
Gene Synonym:
Exon (50)5637..5732
Gene:DYSF
Gene Synonym:
Exon (51)5733..5874
Gene:DYSF
Gene Synonym:
Exon (52)5875..5974
Gene:DYSF
Gene Synonym:
Exon (53)5975..6153
Gene:DYSF
Gene Synonym:
Exon (54)6154..6263
Gene:DYSF
Gene Synonym:
Exon (55)6264..6411
Gene:DYSF
Gene Synonym:
Exon (56)6412..6721
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130983
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130983

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGCC TGCAGGTGCT
GTCAAGCCTT CGAAAGCCTC AGACTTGGAT GACTACCTGG GCTTCCTCCC CACTTTTGGG
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CAGGCTTCCC AGACCCCTAC
ACAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTCTCC
CTGGAGACCA AGCTGGTGGA GCACAGTGAA CAGAAGGTGG AGGACCTTCC TGCGGATGAC
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC
TCAGCCACCA TGCTGCAGGA TGTGGATGAT GCCATCCAGT TTGAGGTCAG CATCGGGAAC
TACGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTACAGCCGT
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GTAACGTGAA ACCTGTGGTG
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG
TGCTCCACGG AGGACGTGGA CTCGCTGGTG GCTCAGCTGA CGGATGAGCT CATCGCAGGC
TGCAGCCAGC CTCTGGGTGA CATCCATGAG ACACCCTCTG CCACCCACCT GGACCAGTAC
CTGTACCAGC TGCGCACCCA TCACCTGAGC CAAATCACTG AGGCTGCCCT GGCCCTGAAG
CTCGGCCACA GTGAGCTCCC TGCAGCTCTG GAGCAGGCGG AGGACTGGCT CCTGCGTCTG
CGTGCCCTGG CAGAGGAGCC CCAGAACAGC CTGCCGGACA TCGTCATCTG GATGCTGCAG
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGCTACAGA CAATCTTTCT GAAATATCCG
ATGGAGAAGG TGCCTGGCGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG
CTCTCAGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT
GAAACCTATG AGAACGAGAC TAAGTTGGCC CTTGTTGGGA ACTGGGGCAC AACGGGCCTC
ACCTACCCCA AGTTTTCTGA CGTCACGGGC AAGATCAAGC TACCCAAGGA CAGCTTCCGC
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACTCTGCTC
CATGACATGG ACGCCGGTCA CCTGAGCTTC GTGGAAGAGG TGTTTGAGAA CCAGACCCGG
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG
GTGCTTCCCA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC
CCGGAGCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAAA TGGAAGCACT GAAAAGGCAC
AGGCAGGCGG AGGCGGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG TCGCATGGAG
CCACTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTTG AGGGGGCCCT GGGCGGCGTG
ATGGATGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTCGG TGTGAACAGA
CCCACGATTT CCTGCATATT CGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTTGGCG AGCCGGCCAC AGTTGCTGAG
CAACCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTATGGTGC AGACGAGTTT
ATGGGTCGCT GCATCTGTCA ACCGAGTCTG GAACGGATGC CACGGCTGGC CTGGTTCCCA
CTGACGAGGG GCAGCCAGCC GTCGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC AAGGATCCTG
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGAT CCTGGCATGG
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAACATCT CCTCCCCCAG CCTCGTGGTA
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC
TTTGACATCT GCACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC
CCCATCACCG TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC
CCCACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGATTGGTGG
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACCT GGAGAAGGAT
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT
CCATCTGTGA TTGGTGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCG CCCAGGGACC CCAGGAGTGC
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGAAAG
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
ATCCCCTGCA CGCTGGAGCC CGTATTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT
CTGGAGAAGG ACCTAAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA GGACGAAAAG
ATCGGTGAGA CGGTCGTCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGG GGCTCGCTGT
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CATAGAGTCA AGGCACCTGT GTACCGGACA
GACCGTGTAA TGTTTCAGGA TAAAGAATAT TCCATTGAAG AGATAGAGGC TGGCAGGATC
CCAAACCCAC ACCTGGGCCC AGTGGAGGAG CGTCTGGCTC TGCATGTGCT TCAGCAGCAG
GGCCTGGTCC CGGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATC
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTATTTCCGA AGGCCCTGGG GCGGCCTGGA
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG
AATACCAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT
CGTTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG
CCAGCTGAGC AAGTCTGTAC CATTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT
GAGAGCAAAA TCCCAGCACG AGTGGTGTTC CAGATCTGGG ACAATGACAA GTTCTCCTTT
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA
GCCAAGAAGT GCTCCTTGGA CCAGCTGGAT GATGCTTTCC ACCCAGAATG GTTTGTGTCC
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTG TAGCAGAAGA GGGTGAGAAG
AAAATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAGCAGAGAG TGAGCATGAG
GAGCGGCCTG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA
AGGCGCCCCG ACACCTCCTT CCTGTGGTTT ACCTCCCCAT ACAAGACCAT GAAGTTCATC
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28600
Clone ID Related Accession (Same CDS sequence) NM_001130984
Accession Version NM_001130984.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6267bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU515165.1, AF075575.1, DB292971.1, EU515157.1 and AK074649.1. Transcript Variant: This variant (5) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 5 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515165.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACA
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGATGC CATCCAGTTT
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC
ACCACTCAGT ACAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGACA TCCATGAGAC ACCCTCTGCC
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCATC ACCTGAGCCA AATCACTGAG
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTACAGACA
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGCGCCC GGATGCCAGT GCAGATACGG
GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACTA AGTTGGCCCT TGTTGGGAAC
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACGGGCAA GATCAAGCTA
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG
GGGGCCCTGG GCGGCGTGAT GGATGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG
AGCTTCGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCA
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC
ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC
TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA
GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC
TCCTACCTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG
CAGGAGGAGA CAGAAGATCC ATCTGTGATT GGTGAATTTA AGGGCCTCTT CAAAATTTAT
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCGCC
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC
AAGGACCCCA ATGGAAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG
AGTGACCAGG ATAACTACAT CCCCTGCACG CTGGAGCCCG TATTTGGAAA GATGTTCGAG
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTAAAGATCA CTCTCTATGA CTATGACCTC
CTCTCCAAGG ACGAAAAGAT CGGTGAGACG GTCGTCGACC TGGAGAACAG GCTGCTGTCC
AAGTTTGGGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA TAGAGTCAAG
GCACCTGTGT ACCGGACAGA CCGTGTAATG TTTCAGGATA AAGAATATTC CATTGAAGAG
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG
CATGTGCTTC AGCAGCAGGG CCTGGTCCCG GAGCACGTGG AGTCACGGCC CCTCTACAGC
CCCCTGCAGC CAGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT ATTTCCGAAG
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA
AAGACAGACG TGCATTATCG TTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT
TTCCCCTTCG ACTACCTGCC AGCTGAGCAA GTCTGTACCA TTGCCAAGAA GGATGCCTTC
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGGTGTTCCA GATCTGGGAC
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG
CCCAAGCCAG CCAAGACAGC CAAGAAGTGC TCCTTGGACC AGCTGGATGA TGCTTTCCAC
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA
GCAGAAGAGG GTGAGAAGAA AATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA
GCAGAGAGTG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTTAC CTCCCCATAC
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124456.1
CDS142..6408
Misc Feature(1)154..543(+)
Misc Feature(2)793..1122(+)
Misc Feature(3)1051..1266(+)
Misc Feature(4)1282..1764(+)
Misc Feature(5)1375..1587(+)
Misc Feature(6)2182..2379(+)
Misc Feature(7)2458..2685(+)
Misc Feature(8)2725..2901(+)
Misc Feature(9)2938..3108(+)
Misc Feature(10)3556..3942(+)
Misc Feature(11)3604..3798(+)
Misc Feature(12)4900..5271(+)
Misc Feature(13)4945..5133(+)
Misc Feature(14)5602..5997(+)
Exon (1)1..232
Gene:DYSF
Gene Synonym:
Exon (2)233..288
Gene:DYSF
Gene Synonym:
Exon (3)289..380
Gene:DYSF
Gene Synonym:
Exon (4)381..486
Gene:DYSF
Gene Synonym:
Exon (5)487..601
Gene:DYSF
Gene Synonym:
Exon (6)602..807
Gene:DYSF
Gene Synonym:
Exon (7)808..936
Gene:DYSF
Gene Synonym:
Exon (8)937..999
Gene:DYSF
Gene Synonym:
Exon (9)1000..1050
Gene:DYSF
Gene Synonym:
Exon (10)1051..1081
Gene:DYSF
Gene Synonym:
Exon (11)1082..1197
Gene:DYSF
Gene Synonym:
Exon (12)1198..1324
Gene:DYSF
Gene Synonym:
Exon (13)1325..1428
Gene:DYSF
Gene Synonym:
Exon (14)1429..1497
Gene:DYSF
Gene Synonym:
Exon (15)1498..1541
Gene:DYSF
Gene Synonym:
Exon (16)1542..1624
Gene:DYSF
Gene Synonym:
Exon (17)1625..1740
Gene:DYSF
Gene Synonym:
Exon (18)1741..1854
Gene:DYSF
Gene Synonym:
Exon (19)1855..2032
Gene:DYSF
Gene Synonym:
Exon (20)2033..2157
Gene:DYSF
Gene Synonym:
Exon (21)2158..2264
Gene:DYSF
Gene Synonym:
Exon (22)2265..2457
Gene:DYSF
Gene Synonym:
Exon (23)2458..2613
Gene:DYSF
Gene Synonym:
Exon (24)2614..2745
Gene:DYSF
Gene Synonym:
Exon (25)2746..2912
Gene:DYSF
Gene Synonym:
Exon (26)2913..3027
Gene:DYSF
Gene Synonym:
Exon (27)3028..3133
Gene:DYSF
Gene Synonym:
Exon (28)3134..3276
Gene:DYSF
Gene Synonym:
Exon (29)3277..3450
Gene:DYSF
Gene Synonym:
Exon (30)3451..3544
Gene:DYSF
Gene Synonym:
Exon (31)3545..3622
Gene:DYSF
Gene Synonym:
Exon (32)3623..3804
Gene:DYSF
Gene Synonym:
Exon (33)3805..3945
Gene:DYSF
Gene Synonym:
Exon (34)3946..3975
Gene:DYSF
Gene Synonym:
Exon (35)3976..4005
Gene:DYSF
Gene Synonym:
Exon (36)4006..4107
Gene:DYSF
Gene Synonym:
Exon (37)4108..4269
Gene:DYSF
Gene Synonym:
Exon (38)4270..4435
Gene:DYSF
Gene Synonym:
Exon (39)4436..4512
Gene:DYSF
Gene Synonym:
Exon (40)4513..4575
Gene:DYSF
Gene Synonym:
Exon (41)4576..4674
Gene:DYSF
Gene Synonym:
Exon (42)4675..4803
Gene:DYSF
Gene Synonym:
Exon (43)4804..4959
Gene:DYSF
Gene Synonym:
Exon (44)4960..5051
Gene:DYSF
Gene Synonym:
Exon (45)5052..5222
Gene:DYSF
Gene Synonym:
Exon (46)5223..5365
Gene:DYSF
Gene Synonym:
Exon (47)5366..5505
Gene:DYSF
Gene Synonym:
Exon (48)5506..5594
Gene:DYSF
Gene Synonym:
Exon (49)5595..5690
Gene:DYSF
Gene Synonym:
Exon (50)5691..5832
Gene:DYSF
Gene Synonym:
Exon (51)5833..5932
Gene:DYSF
Gene Synonym:
Exon (52)5933..6111
Gene:DYSF
Gene Synonym:
Exon (53)6112..6221
Gene:DYSF
Gene Synonym:
Exon (54)6222..6369
Gene:DYSF
Gene Synonym:
Exon (55)6370..6679
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130984
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130984

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACA
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGATGC CATCCAGTTT
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC
ACCACTCAGT ACAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGACA TCCATGAGAC ACCCTCTGCC
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCATC ACCTGAGCCA AATCACTGAG
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTACAGACA
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGCGCCC GGATGCCAGT GCAGATACGG
GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACTA AGTTGGCCCT TGTTGGGAAC
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACGGGCAA GATCAAGCTA
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG
GGGGCCCTGG GCGGCGTGAT GGATGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG
AGCTTCGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCA
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC
ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC
TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC
GGTCTGTCGA GCTTGGCCCC CACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA
GAGTTCATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC
TCCTACCTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG
CAGGAGGAGA CAGAAGATCC ATCTGTGATT GGTGAATTTA AGGGCCTCTT CAAAATTTAT
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCGCC
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC
AAGGACCCCA ATGGAAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG
AGTGACCAGG ATAACTACAT CCCCTGCACG CTGGAGCCCG TATTTGGAAA GATGTTCGAG
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTAAAGATCA CTCTCTATGA CTATGACCTC
CTCTCCAAGG ACGAAAAGAT CGGTGAGACG GTCGTCGACC TGGAGAACAG GCTGCTGTCC
AAGTTTGGGG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC GAACCAGTGG
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCA TAGAGTCAAG
GCACCTGTGT ACCGGACAGA CCGTGTAATG TTTCAGGATA AAGAATATTC CATTGAAGAG
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG
CATGTGCTTC AGCAGCAGGG CCTGGTCCCG GAGCACGTGG AGTCACGGCC CCTCTACAGC
CCCCTGCAGC CAGACATCGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT ATTTCCGAAG
GCCCTGGGGC GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC
CTGCGTTGTA TTATCTGGAA TACCAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA
AAGACAGACG TGCATTATCG TTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT
TTCCCCTTCG ACTACCTGCC AGCTGAGCAA GTCTGTACCA TTGCCAAGAA GGATGCCTTC
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGGTGTTCCA GATCTGGGAC
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG
CCCAAGCCAG CCAAGACAGC CAAGAAGTGC TCCTTGGACC AGCTGGATGA TGCTTTCCAC
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTGTA
GCAGAAGAGG GTGAGAAGAA AATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA
GCAGAGAGTG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTTAC CTCCCCATAC
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28617
Clone ID Related Accession (Same CDS sequence) NM_001130985
Accession Version NM_001130985.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6297bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU515164.1, AF075575.1 and DB292971.1. Transcript Variant: This variant (4) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 4 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515164.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACAGGCTTCC CAGACCCCTA CACAGAGCTC
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC
ATGCTGCAGG ATGTGGATGA TGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG
CCTCTGGGTG ACATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG
CTGCGCACCC ATCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC
TACTGTGGCA AGAATTGTGG GAAGCTACAG ACAATCTTTC TGAAATATCC GATGGAGAAG
GTGCCTGGCG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCAGTG
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT
GAGAACGAGA CTAAGTTGGC CCTTGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC
AAGTTTTCTG ACGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAC
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTCG GTGTGAACAG ACCCACGATT
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC
TGCATCTGTC AACCGAGTCT GGAACGGATG CCACGGCTGG CCTGGTTCCC ACTGACGAGG
GGCAGCCAGC CGTCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGA TCCTGGCATG GGGCCTGCGG
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTGGG
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACCTGG AGAAGGATTT TGACACCCTG
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTGATT
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGAAAGTG TGATCCTTAC
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG
GTCGTCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CAAGAAGTGC
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124457.1
CDS142..6438
Misc Feature(1)154..543(+)
Misc Feature(2)886..1215(+)
Misc Feature(3)1144..1359(+)
Misc Feature(4)1375..1857(+)
Misc Feature(5)1468..1680(+)
Misc Feature(6)2275..2472(+)
Misc Feature(7)2551..2778(+)
Misc Feature(8)2818..2994(+)
Misc Feature(9)3031..3201(+)
Misc Feature(10)3649..4035(+)
Misc Feature(11)3697..3891(+)
Misc Feature(12)4930..5301(+)
Misc Feature(13)4975..5163(+)
Misc Feature(14)5632..6027(+)
Exon (1)1..232
Gene:DYSF
Gene Synonym:
Exon (2)233..288
Gene:DYSF
Gene Synonym:
Exon (3)289..380
Gene:DYSF
Gene Synonym:
Exon (4)381..486
Gene:DYSF
Gene Synonym:
Exon (5)487..601
Gene:DYSF
Gene Synonym:
Exon (6)602..694
Gene:DYSF
Gene Synonym:
Exon (7)695..900
Gene:DYSF
Gene Synonym:
Exon (8)901..1029
Gene:DYSF
Gene Synonym:
Exon (9)1030..1092
Gene:DYSF
Gene Synonym:
Exon (10)1093..1143
Gene:DYSF
Gene Synonym:
Exon (11)1144..1174
Gene:DYSF
Gene Synonym:
Exon (12)1175..1290
Gene:DYSF
Gene Synonym:
Exon (13)1291..1417
Gene:DYSF
Gene Synonym:
Exon (14)1418..1521
Gene:DYSF
Gene Synonym:
Exon (15)1522..1590
Gene:DYSF
Gene Synonym:
Exon (16)1591..1634
Gene:DYSF
Gene Synonym:
Exon (17)1635..1717
Gene:DYSF
Gene Synonym:
Exon (18)1718..1833
Gene:DYSF
Gene Synonym:
Exon (19)1834..1947
Gene:DYSF
Gene Synonym:
Exon (20)1948..2125
Gene:DYSF
Gene Synonym:
Exon (21)2126..2250
Gene:DYSF
Gene Synonym:
Exon (22)2251..2357
Gene:DYSF
Gene Synonym:
Exon (23)2358..2550
Gene:DYSF
Gene Synonym:
Exon (24)2551..2706
Gene:DYSF
Gene Synonym:
Exon (25)2707..2838
Gene:DYSF
Gene Synonym:
Exon (26)2839..3005
Gene:DYSF
Gene Synonym:
Exon (27)3006..3120
Gene:DYSF
Gene Synonym:
Exon (28)3121..3226
Gene:DYSF
Gene Synonym:
Exon (29)3227..3369
Gene:DYSF
Gene Synonym:
Exon (30)3370..3543
Gene:DYSF
Gene Synonym:
Exon (31)3544..3637
Gene:DYSF
Gene Synonym:
Exon (32)3638..3715
Gene:DYSF
Gene Synonym:
Exon (33)3716..3897
Gene:DYSF
Gene Synonym:
Exon (34)3898..4038
Gene:DYSF
Gene Synonym:
Exon (35)4039..4068
Gene:DYSF
Gene Synonym:
Exon (36)4069..4098
Gene:DYSF
Gene Synonym:
Exon (37)4099..4200
Gene:DYSF
Gene Synonym:
Exon (38)4201..4362
Gene:DYSF
Gene Synonym:
Exon (39)4363..4528
Gene:DYSF
Gene Synonym:
Exon (40)4529..4605
Gene:DYSF
Gene Synonym:
Exon (41)4606..4704
Gene:DYSF
Gene Synonym:
Exon (42)4705..4833
Gene:DYSF
Gene Synonym:
Exon (43)4834..4989
Gene:DYSF
Gene Synonym:
Exon (44)4990..5081
Gene:DYSF
Gene Synonym:
Exon (45)5082..5252
Gene:DYSF
Gene Synonym:
Exon (46)5253..5395
Gene:DYSF
Gene Synonym:
Exon (47)5396..5535
Gene:DYSF
Gene Synonym:
Exon (48)5536..5624
Gene:DYSF
Gene Synonym:
Exon (49)5625..5720
Gene:DYSF
Gene Synonym:
Exon (50)5721..5862
Gene:DYSF
Gene Synonym:
Exon (51)5863..5962
Gene:DYSF
Gene Synonym:
Exon (52)5963..6141
Gene:DYSF
Gene Synonym:
Exon (53)6142..6251
Gene:DYSF
Gene Synonym:
Exon (54)6252..6399
Gene:DYSF
Gene Synonym:
Exon (55)6400..6709
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130985
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130985

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACAGGCTTCC CAGACCCCTA CACAGAGCTC
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC
ATGCTGCAGG ATGTGGATGA TGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG
CCTCTGGGTG ACATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG
CTGCGCACCC ATCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC
TACTGTGGCA AGAATTGTGG GAAGCTACAG ACAATCTTTC TGAAATATCC GATGGAGAAG
GTGCCTGGCG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCAGTG
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT
GAGAACGAGA CTAAGTTGGC CCTTGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC
AAGTTTTCTG ACGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAC
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTCG GTGTGAACAG ACCCACGATT
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC
TGCATCTGTC AACCGAGTCT GGAACGGATG CCACGGCTGG CCTGGTTCCC ACTGACGAGG
GGCAGCCAGC CGTCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGA TCCTGGCATG GGGCCTGCGG
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTGGG
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTCATCG ATTGGTGGAG CAAATTCTTT
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACCTGG AGAAGGATTT TGACACCCTG
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTGATT
GGTGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG
CCCCCAAGAC AGTTCCACCA GCTGGCCGCC CAGGGACCCC AGGAGTGCTT GGTCCGTATC
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGAAAGTG TGATCCTTAC
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG
CTGGAGCCCG TATTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC
CTAAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAGG ACGAAAAGAT CGGTGAGACG
GTCGTCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGGGG CTCGCTGTGG ACTCCCACAG
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC
CACCTCTTCT GCCAGCAGCA TAGAGTCAAG GCACCTGTGT ACCGGACAGA CCGTGTAATG
TTTCAGGATA AAGAATATTC CATTGAAGAG ATAGAGGCTG GCAGGATCCC AAACCCACAC
CTGGGCCCAG TGGAGGAGCG TCTGGCTCTG CATGTGCTTC AGCAGCAGGG CCTGGTCCCG
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATCGA GCAGGGGAAG
CTGCAGATGT GGGTCGACCT ATTTCCGAAG GCCCTGGGGC GGCCTGGACC TCCCTTCAAC
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACCAGAGAT
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG TTCCCTGGGA
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC AGCTGAGCAA
GTCTGTACCA TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC
CCAGCACGAG TGGTGTTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CAAGAAGTGC
TCCTTGGACC AGCTGGATGA TGCTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG
AAAACAGTGA AGGGCTGGTG GCCCTGTGTA GCAGAAGAGG GTGAGAAGAA AATACTGGCG
GGCAAGCTGG AAATGACCTT GGAGATTGTA GCAGAGAGTG AGCATGAGGA GCGGCCTGCT
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC
ACCTCCTTCC TGTGGTTTAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28615
Clone ID Related Accession (Same CDS sequence) NM_001130986
Accession Version NM_001130986.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6204bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU515162.1, AF075575.1 and DB292971.1. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 3 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACA
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGATGC CATCCAGTTT
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC
ACCACTCAGT ACAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGACA TCCATGAGAC ACCCTCTGCC
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCATC ACCTGAGCCA AATCACTGAG
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTACAGACA
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGCGCCC GGATGCCAGT GCAGATACGG
GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACTA AGTTGGCCCT TGTTGGGAAC
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACGGGCAA GATCAAGCTA
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG
GGGGCCCTGG GCGGCGTGAT GGATGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG
AGCTTCGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCA
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC
ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC
TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG
GAGGAGACAG AAGATCCATC TGTGATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG
GACCCCAATG GAAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC
TCCAAGGACG AAAAGATCGG TGAGACGGTC GTCGACCTGG AGAACAGGCT GCTGTCCAAG
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC
AAGCCAGCCA AGACAGCCAA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124458.1
CDS142..6345
Misc Feature(1)154..543(+)
Misc Feature(2)793..1122(+)
Misc Feature(3)1051..1266(+)
Misc Feature(4)1282..1764(+)
Misc Feature(5)1375..1587(+)
Misc Feature(6)2182..2379(+)
Misc Feature(7)2458..2685(+)
Misc Feature(8)2725..2901(+)
Misc Feature(9)2938..3108(+)
Misc Feature(10)3556..3942(+)
Misc Feature(11)3604..3798(+)
Misc Feature(12)4837..5208(+)
Misc Feature(13)4882..5070(+)
Misc Feature(14)5539..5934(+)
Misc Feature(15)6241..6303(+)
Exon (1)1..232
Gene:DYSF
Gene Synonym:
Exon (2)233..288
Gene:DYSF
Gene Synonym:
Exon (3)289..380
Gene:DYSF
Gene Synonym:
Exon (4)381..486
Gene:DYSF
Gene Synonym:
Exon (5)487..601
Gene:DYSF
Gene Synonym:
Exon (6)602..807
Gene:DYSF
Gene Synonym:
Exon (7)808..936
Gene:DYSF
Gene Synonym:
Exon (8)937..999
Gene:DYSF
Gene Synonym:
Exon (9)1000..1050
Gene:DYSF
Gene Synonym:
Exon (10)1051..1081
Gene:DYSF
Gene Synonym:
Exon (11)1082..1197
Gene:DYSF
Gene Synonym:
Exon (12)1198..1324
Gene:DYSF
Gene Synonym:
Exon (13)1325..1428
Gene:DYSF
Gene Synonym:
Exon (14)1429..1497
Gene:DYSF
Gene Synonym:
Exon (15)1498..1541
Gene:DYSF
Gene Synonym:
Exon (16)1542..1624
Gene:DYSF
Gene Synonym:
Exon (17)1625..1740
Gene:DYSF
Gene Synonym:
Exon (18)1741..1854
Gene:DYSF
Gene Synonym:
Exon (19)1855..2032
Gene:DYSF
Gene Synonym:
Exon (20)2033..2157
Gene:DYSF
Gene Synonym:
Exon (21)2158..2264
Gene:DYSF
Gene Synonym:
Exon (22)2265..2457
Gene:DYSF
Gene Synonym:
Exon (23)2458..2613
Gene:DYSF
Gene Synonym:
Exon (24)2614..2745
Gene:DYSF
Gene Synonym:
Exon (25)2746..2912
Gene:DYSF
Gene Synonym:
Exon (26)2913..3027
Gene:DYSF
Gene Synonym:
Exon (27)3028..3133
Gene:DYSF
Gene Synonym:
Exon (28)3134..3276
Gene:DYSF
Gene Synonym:
Exon (29)3277..3450
Gene:DYSF
Gene Synonym:
Exon (30)3451..3544
Gene:DYSF
Gene Synonym:
Exon (31)3545..3622
Gene:DYSF
Gene Synonym:
Exon (32)3623..3804
Gene:DYSF
Gene Synonym:
Exon (33)3805..3945
Gene:DYSF
Gene Synonym:
Exon (34)3946..3975
Gene:DYSF
Gene Synonym:
Exon (35)3976..4005
Gene:DYSF
Gene Synonym:
Exon (36)4006..4107
Gene:DYSF
Gene Synonym:
Exon (37)4108..4269
Gene:DYSF
Gene Synonym:
Exon (38)4270..4435
Gene:DYSF
Gene Synonym:
Exon (39)4436..4512
Gene:DYSF
Gene Synonym:
Exon (40)4513..4611
Gene:DYSF
Gene Synonym:
Exon (41)4612..4740
Gene:DYSF
Gene Synonym:
Exon (42)4741..4896
Gene:DYSF
Gene Synonym:
Exon (43)4897..4988
Gene:DYSF
Gene Synonym:
Exon (44)4989..5159
Gene:DYSF
Gene Synonym:
Exon (45)5160..5302
Gene:DYSF
Gene Synonym:
Exon (46)5303..5442
Gene:DYSF
Gene Synonym:
Exon (47)5443..5531
Gene:DYSF
Gene Synonym:
Exon (48)5532..5627
Gene:DYSF
Gene Synonym:
Exon (49)5628..5769
Gene:DYSF
Gene Synonym:
Exon (50)5770..5869
Gene:DYSF
Gene Synonym:
Exon (51)5870..6048
Gene:DYSF
Gene Synonym:
Exon (52)6049..6158
Gene:DYSF
Gene Synonym:
Exon (53)6159..6306
Gene:DYSF
Gene Synonym:
Exon (54)6307..6616
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130986
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130986

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC
ACAGAGGACC AGGGACTCAC TGGAGATGAG GCGGAGCCAT TCCTGGATCA AAGCGGAGGC
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTCACGTC CTCCGCCCCA CTACCCCGGG
ATCAAAAGAA AGCGAAGTGC GCCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAT
TTCCAGATCA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CGGGGGTGAA CATCAAGCCT
GTGGTCAAGG TTACCGCTGC AGGGCAGACC AAGCGGACGC GGATCCACAA GGGAAACAGC
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT CTCCTGGGGA GCTGTTTGAT
GAGCCCATCT TTATCACGGT GGTAGACTCT CGTTCTCTCA GGACAGATGC TCTCCTCGGG
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGCTA CCTGAAAACA
AGCCTTTGTG TGCTGGGGCC TGGGGACGAA GCGCCTCTGG AGAGAAAAGA CCCCTCTGAA
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTAGCCCT GCGAGGAGCC
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCGC AGATGGACGA TGCCGTGATG
GACAACGTGA AACAGATCTT TGGCTTCGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT
GTGGAGGTCA GCTTTGCGGG GAAAATGCTG TGCAGCAAGA TCTTGGAGAA GACGGCCAAC
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG CGAAAAAATG
AGGATTCGTA TCATAGACTG GGACCGCCTG ACTCACAATG ACATCGTGGC TACCACCTAC
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CTACCTGGGC
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACA
GGCTTCCCAG ACCCCTACAC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT
GGCCGGCTTC TGCTCTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAACA GAAGGTGGAG
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC
CTGTTTGCGG CCTTCTACTC AGCCACCATG CTGCAGGATG TGGATGATGC CATCCAGTTT
GAGGTCAGCA TCGGGAACTA CGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC
ACCACTCAGT ACAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGT
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CGCTGGTGGC TCAGCTGACG
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGACA TCCATGAGAC ACCCTCTGCC
ACCCACCTGG ACCAGTACCT GTACCAGCTG CGCACCCATC ACCTGAGCCA AATCACTGAG
GCTGCCCTGG CCCTGAAGCT CGGCCACAGT GAGCTCCCTG CAGCTCTGGA GCAGGCGGAG
GACTGGCTCC TGCGTCTGCG TGCCCTGGCA GAGGAGCCCC AGAACAGCCT GCCGGACATC
GTCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GCTACAGACA
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGCGCCC GGATGCCAGT GCAGATACGG
GTCAAGCTGT GGTTTGGGCT CTCAGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACGAGACTA AGTTGGCCCT TGTTGGGAAC
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACGGGCAA GATCAAGCTA
CCCAAGGACA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT
CCGGAGAAGA CTCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTCGT GGAAGAGGTG
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC
GATGTGAACG GGGAGAAGGT GCTTCCCAAG GATGACATTG AGTGCCCACT GGGCTGGAAG
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG
TATAGCATCA CCATCCCCCC GGAGCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAAATG
GAAGCACTGA AAAGGCACAG GCAGGCGGAG GCGGAGGGCG AGGGCTGGGA GTACGCCTCT
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC
TGGCGCCGTC GCATGGAGCC ACTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTTGAG
GGGGCCCTGG GCGGCGTGAT GGATGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG
AGCTTCGGTG TGAACAGACC CACGATTTCC TGCATATTCG ACTATGGGAA CCGCTACCAT
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTTGGCGAG
CCGGCCACAG TTGCTGAGCA ACCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT
TATGGTGCAG ACGAGTTTAT GGGTCGCTGC ATCTGTCAAC CGAGTCTGGA ACGGATGCCA
CGGCTGGCCT GGTTCCCACT GACGAGGGGC AGCCAGCCGT CGGGGGAGCT GCTGGCCTCT
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG
GAGACATCAA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC
ATCGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAACATCTCC
TCCCCCAGCC TCGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC
CTCCGGAAGA ACCCCAACTT TGACATCTGC ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG
GAGGAGCTCT ACTGCCCCCC CATCACCGTC AAGGTCATCG ATAACCGCCA GTTTGGCCGC
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG
TTCATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC
TACCTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG
GAGGAGACAG AAGATCCATC TGTGATTGGT GAATTTAAGG GCCTCTTCAA AATTTATCCC
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCCCAG
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG
GACCCCAATG GAAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT
GACCAGGATA ACTACATCCC CTGCACGCTG GAGCCCGTAT TTGGAAAGAT GTTCGAGCTG
ACCTGCACTC TGCCTCTGGA GAAGGACCTA AAGATCACTC TCTATGACTA TGACCTCCTC
TCCAAGGACG AAAAGATCGG TGAGACGGTC GTCGACCTGG AGAACAGGCT GCTGTCCAAG
TTTGGGGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCATAG AGTCAAGGCA
CCTGTGTACC GGACAGACCG TGTAATGTTT CAGGATAAAG AATATTCCAT TGAAGAGATA
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCAGTGG AGGAGCGTCT GGCTCTGCAT
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC
CTGCAGCCAG ACATCGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTATT TCCGAAGGCC
CTGGGGCGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG
CGTTGTATTA TCTGGAATAC CAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG
ACAGACGTGC ATTATCGTTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC
CCCTTCGACT ACCTGCCAGC TGAGCAAGTC TGTACCATTG CCAAGAAGGA TGCCTTCTGG
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGG TGTTCCAGAT CTGGGACAAT
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC
AAGCCAGCCA AGACAGCCAA GAAGTGCTCC TTGGACCAGC TGGATGATGC TTTCCACCCA
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTGTAGCA
GAAGAGGGTG AGAAGAAAAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAGCA
GAGAGTGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTTACCTC CCCATACAAG
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28601
Clone ID Related Accession (Same CDS sequence) NM_001130987
Accession Version NM_001130987.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6360bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dysferlin isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU515166.1, AF075575.1, DB292971.1, EU515157.1 and AK074649.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU515166.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACAGGCTTCC CAGACCCCTA CACAGAGCTC
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC
ATGCTGCAGG ATGTGGATGA TGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG
CCTCTGGGTG ACATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG
CTGCGCACCC ATCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC
TACTGTGGCA AGAATTGTGG GAAGCTACAG ACAATCTTTC TGAAATATCC GATGGAGAAG
GTGCCTGGCG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCAGTG
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT
GAGAACGAGA CTAAGTTGGC CCTTGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC
AAGTTTTCTG ACGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAC
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTCG GTGTGAACAG ACCCACGATT
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC
TGCATCTGTC AACCGAGTCT GGAACGGATG CCACGGCTGG CCTGGTTCCC ACTGACGAGG
GGCAGCCAGC CGTCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGA TCCTGGCATG GGGCCTGCGG
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTGGG
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCCACTAAC
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACC TGGAGAAGGA TTTTGACACC
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTG
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGAAA GTGTGATCCT
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG
ACGGTCGTCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCAAGAAG
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001124459.1
CDS142..6501
Misc Feature(1)154..543(+)
Misc Feature(2)886..1215(+)
Misc Feature(3)1144..1359(+)
Misc Feature(4)1375..1857(+)
Misc Feature(5)1468..1680(+)
Misc Feature(6)2275..2472(+)
Misc Feature(7)2551..2778(+)
Misc Feature(8)2818..2994(+)
Misc Feature(9)3031..3201(+)
Misc Feature(10)3649..4035(+)
Misc Feature(11)3697..3891(+)
Misc Feature(12)4993..5364(+)
Misc Feature(13)5038..5226(+)
Misc Feature(14)5695..6090(+)
Exon (1)1..232
Gene:DYSF
Gene Synonym:
Exon (2)233..288
Gene:DYSF
Gene Synonym:
Exon (3)289..380
Gene:DYSF
Gene Synonym:
Exon (4)381..486
Gene:DYSF
Gene Synonym:
Exon (5)487..601
Gene:DYSF
Gene Synonym:
Exon (6)602..694
Gene:DYSF
Gene Synonym:
Exon (7)695..900
Gene:DYSF
Gene Synonym:
Exon (8)901..1029
Gene:DYSF
Gene Synonym:
Exon (9)1030..1092
Gene:DYSF
Gene Synonym:
Exon (10)1093..1143
Gene:DYSF
Gene Synonym:
Exon (11)1144..1174
Gene:DYSF
Gene Synonym:
Exon (12)1175..1290
Gene:DYSF
Gene Synonym:
Exon (13)1291..1417
Gene:DYSF
Gene Synonym:
Exon (14)1418..1521
Gene:DYSF
Gene Synonym:
Exon (15)1522..1590
Gene:DYSF
Gene Synonym:
Exon (16)1591..1634
Gene:DYSF
Gene Synonym:
Exon (17)1635..1717
Gene:DYSF
Gene Synonym:
Exon (18)1718..1833
Gene:DYSF
Gene Synonym:
Exon (19)1834..1947
Gene:DYSF
Gene Synonym:
Exon (20)1948..2125
Gene:DYSF
Gene Synonym:
Exon (21)2126..2250
Gene:DYSF
Gene Synonym:
Exon (22)2251..2357
Gene:DYSF
Gene Synonym:
Exon (23)2358..2550
Gene:DYSF
Gene Synonym:
Exon (24)2551..2706
Gene:DYSF
Gene Synonym:
Exon (25)2707..2838
Gene:DYSF
Gene Synonym:
Exon (26)2839..3005
Gene:DYSF
Gene Synonym:
Exon (27)3006..3120
Gene:DYSF
Gene Synonym:
Exon (28)3121..3226
Gene:DYSF
Gene Synonym:
Exon (29)3227..3369
Gene:DYSF
Gene Synonym:
Exon (30)3370..3543
Gene:DYSF
Gene Synonym:
Exon (31)3544..3637
Gene:DYSF
Gene Synonym:
Exon (32)3638..3715
Gene:DYSF
Gene Synonym:
Exon (33)3716..3897
Gene:DYSF
Gene Synonym:
Exon (34)3898..4038
Gene:DYSF
Gene Synonym:
Exon (35)4039..4068
Gene:DYSF
Gene Synonym:
Exon (36)4069..4098
Gene:DYSF
Gene Synonym:
Exon (37)4099..4200
Gene:DYSF
Gene Synonym:
Exon (38)4201..4362
Gene:DYSF
Gene Synonym:
Exon (39)4363..4528
Gene:DYSF
Gene Synonym:
Exon (40)4529..4605
Gene:DYSF
Gene Synonym:
Exon (41)4606..4668
Gene:DYSF
Gene Synonym:
Exon (42)4669..4767
Gene:DYSF
Gene Synonym:
Exon (43)4768..4896
Gene:DYSF
Gene Synonym:
Exon (44)4897..5052
Gene:DYSF
Gene Synonym:
Exon (45)5053..5144
Gene:DYSF
Gene Synonym:
Exon (46)5145..5315
Gene:DYSF
Gene Synonym:
Exon (47)5316..5458
Gene:DYSF
Gene Synonym:
Exon (48)5459..5598
Gene:DYSF
Gene Synonym:
Exon (49)5599..5687
Gene:DYSF
Gene Synonym:
Exon (50)5688..5783
Gene:DYSF
Gene Synonym:
Exon (51)5784..5925
Gene:DYSF
Gene Synonym:
Exon (52)5926..6025
Gene:DYSF
Gene Synonym:
Exon (53)6026..6204
Gene:DYSF
Gene Synonym:
Exon (54)6205..6314
Gene:DYSF
Gene Synonym:
Exon (55)6315..6462
Gene:DYSF
Gene Synonym:
Exon (56)6463..6772
Gene:DYSF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001130987
Organism Homo sapiens (human)
Definition Homo sapiens dysferlin (DYSF), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130987

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG
AACAGCGTGA ACCCTGTATG GAATGAGGGA TTTGAATGGG ACCTCAAGGG CATCCCCCTG
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG
GAGCCCTCCC CGACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC
GAGACTTGGT CCCTGCTCAG TGACAGCACC ATGGACACGA GATACTCTGG AAAGAAGTGG
CCGGCCCCCA CGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT
GAGGCGGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA
CTACCTTCAC GTCCTCCGCC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCGCCTACA
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GATTTCCAGA TCAGGGTCCA GGTGATCGAG
GGGCGCCAGC TGCCGGGGGT GAACATCAAG CCTGTGGTCA AGGTTACCGC TGCAGGGCAG
ACCAAGCGGA CGCGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC
AACTTGTTTG ACTCTCCTGG GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC
TCTCGTTCTC TCAGGACAGA TGCTCTCCTC GGGGAGTTCC GGATGGACGT GGGCACCATT
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC
TTCTCTGCTG GGGCCAGAGG CTACCTGAAA ACAAGCCTTT GTGTGCTGGG GCCTGGGGAC
GAAGCGCCTC TGGAGAGAAA AGACCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG
CTCCGGCCCA CAGGCGTAGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC
GAGGACTTGC CGCAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTC
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG
CTGTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG
CCTGCCATGT TTCCCTCCAT GTGCGAAAAA ATGAGGATTC GTATCATAGA CTGGGACCGC
CTGACTCACA ATGACATCGT GGCTACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT
GGAGGAGAAA TAGAAGTGGA TGACTACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACAGGCTTCC CAGACCCCTA CACAGAGCTC
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTCTC CCTGGAGACC
AAGCTGGTGG AGCACAGTGA ACAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCAGCCACC
ATGCTGCAGG ATGTGGATGA TGCCATCCAG TTTGAGGTCA GCATCGGGAA CTACGGGAAC
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTACAGCCG TGCAGTCTTT
GACGGGTGCC ACTACTACTA CCTACCCTGG GGTAACGTGA AACCTGTGGT GGTGCTGTCA
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG
GAGGACGTGG ACTCGCTGGT GGCTCAGCTG ACGGATGAGC TCATCGCAGG CTGCAGCCAG
CCTCTGGGTG ACATCCATGA GACACCCTCT GCCACCCACC TGGACCAGTA CCTGTACCAG
CTGCGCACCC ATCACCTGAG CCAAATCACT GAGGCTGCCC TGGCCCTGAA GCTCGGCCAC
AGTGAGCTCC CTGCAGCTCT GGAGCAGGCG GAGGACTGGC TCCTGCGTCT GCGTGCCCTG
GCAGAGGAGC CCCAGAACAG CCTGCCGGAC ATCGTCATCT GGATGCTGCA GGGAGACAAG
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC
TACTGTGGCA AGAATTGTGG GAAGCTACAG ACAATCTTTC TGAAATATCC GATGGAGAAG
GTGCCTGGCG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCAGTG
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT
GAGAACGAGA CTAAGTTGGC CCTTGTTGGG AACTGGGGCA CAACGGGCCT CACCTACCCC
AAGTTTTCTG ACGTCACGGG CAAGATCAAG CTACCCAAGG ACAGCTTCCG CCCCTCGGCC
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACTCTGCT CCATGACATG
GACGCCGGTC ACCTGAGCTT CGTGGAAGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC
AAGGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGAGCGG
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAA ATGGAAGCAC TGAAAAGGCA CAGGCAGGCG
GAGGCGGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GTCGCATGGA GCCACTGGAG
AAGACGGGGC CTGCAGCTGT GTTTGCCCTT GAGGGGGCCC TGGGCGGCGT GATGGATGAC
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTCG GTGTGAACAG ACCCACGATT
TCCTGCATAT TCGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG
CTCATCTTCT ACGAGATCGA GATCTTTGGC GAGCCGGCCA CAGTTGCTGA GCAACCGCCC
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTATGGTG CAGACGAGTT TATGGGTCGC
TGCATCTGTC AACCGAGTCT GGAACGGATG CCACGGCTGG CCTGGTTCCC ACTGACGAGG
GGCAGCCAGC CGTCGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CAAGGATCCT GGATGAGTCT
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGA TCCTGGCATG GGGCCTGCGG
AACATGAAGA GTTACCAGCT GGCCAACATC TCCTCCCCCA GCCTCGTGGT AGAGTGTGGG
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC
TGCACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC
GTCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCCACTAAC
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTCA TCGATTGGTG GAGCAAATTC
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACC TGGAGAAGGA TTTTGACACC
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTG
ATTGGTGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCCCAGGGAC CCCAGGAGTG CTTGGTCCGT
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGAAA GTGTGATCCT
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC
ACGCTGGAGC CCGTATTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG
GACCTAAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AGGACGAAAA GATCGGTGAG
ACGGTCGTCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG GGGCTCGCTG TGGACTCCCA
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC
CTCCACCTCT TCTGCCAGCA GCATAGAGTC AAGGCACCTG TGTACCGGAC AGACCGTGTA
ATGTTTCAGG ATAAAGAATA TTCCATTGAA GAGATAGAGG CTGGCAGGAT CCCAAACCCA
CACCTGGGCC CAGTGGAGGA GCGTCTGGCT CTGCATGTGC TTCAGCAGCA GGGCCTGGTC
CCGGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT CGAGCAGGGG
AAGCTGCAGA TGTGGGTCGA CCTATTTCCG AAGGCCCTGG GGCGGCCTGG ACCTCCCTTC
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACCAGA
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGTTCCCTG
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCAGCTGAG
CAAGTCTGTA CCATTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA
ATCCCAGCAC GAGTGGTGTT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCAAGAAG
TGCTCCTTGG ACCAGCTGGA TGATGCTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT GTAGCAGAAG AGGGTGAGAA GAAAATACTG
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAGCAGAGA GTGAGCATGA GGAGCGGCCT
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC
GACACCTCCT TCCTGTGGTT TACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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