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COLQ cDNA ORF clone, Homo sapiens (human)

Gene Symbol COLQ
Entrez Gene ID 8292
Full Name collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase
Synonyms CMS5, EAD
General protein information
Preferred Names
acetylcholinesterase collagenic tail peptide
Names
acetylcholinesterase collagenic tail peptide
AChE Q subunit
acetylcholinesterase-associated collagen
collagenic tail of endplate acetylcholinesterase
single strand of homotrimeric collagen-like tail subunit of asymmetric acetylcholinesterase
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p25

Summary This gene encodes the subunit of a collagen-like molecule associated with acetylcholinesterase in skeletal muscle. Each molecule is composed of three identical subunits. Each subunit contains a proline-rich attachment domain (PRAD) that binds an acetylcholinesterase tetramer to anchor the catalytic subunit of the enzyme to the basal lamina. Mutations in this gene are associated with endplate acetylcholinesterase deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

603033

Disorder Html: Endplate acetylcholinesterase deficiency, 603034 (3)





The following COLQ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COLQ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu16271 NM_080539 Homo sapiens collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant III, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $349.00
OHu19054 NM_005677 Homo sapiens collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $379.00
OHu16232 NM_080538 Homo sapiens collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant II, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu16271
Accession Version NM_080539.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1266bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product acetylcholinesterase collagenic tail peptide isoform III precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF057036.1, BX500613.1, AK128401.1 and AI554171.1. On Apr 25, 2007 this sequence version replaced gi:116805305. Summary: This gene encodes the subunit of a collagen-like molecule associated with acetylcholinesterase in skeletal muscle. Each molecule is composed of three identical subunits. Each subunit contains a proline-rich attachment domain (PRAD) that binds an acetylcholinesterase tetramer to anchor the catalytic subunit of the enzyme to the basal lamina. Mutations in this gene are associated with endplate acetylcholinesterase deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (III) is the only variant missing exon 3. Exon 3 encodes the proline-rich attachment domain; as a result, isoform III is the only isoform lacking this domain. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGTTGTCC TGAATCCAAT GACTTTGGGA ATTTATCTTC AGCTTTTCTT CCTCTCTATC 
GTGTCTCAGC CGACTTTCAT CAACAGCGTT CTTCCAATCT CAGCAGCCCT TCCCAGCCTG
GATCAGAAGA AGCGTGGTGG CCACAAAGCA TGCTGCCTGC TGACGCCTCC TCCACCACCA
CTGTTCCCAC CACCATTCTT CAGAGGTGGC CGAAGTCCGG GTCCACCGGG GCTTCCTGGC
AAGACAGGAC CAAAGGGAGA AAAGGGGGAG CTTGGCCGAC CAGGAAGGAA GGGTAGACCT
GGCCCCCCAG GTGTTCCTGG CATGCCTGGG CCCATCGGTT GGCCAGGCCC TGAAGGACCC
AGGGGTGAAA AAGGTGACCT GGGTATGATG GGCTTGCCAG GGTCAAGAGG ACCAATGGGC
TCCAAGGGCT ACCCTGGATC CAGAGGGGAA AAGGGATCCA GAGGTGAAAA GGGTGACCTG
GGTCCCAAAG GAGAAAAGGG TTTCCCAGGA TTTCCTGGAA TGTTGGGGCA GAAAGGTGAA
ATGGGTCCAA AAGGTGAACC TGGGATAGCA GGACACCGAG GACCCACAGG AAGACCAGGA
AAACGAGGCA AGCAGGGACA GAAAGGGGAT AGTGGAGTTA TGGGCCCACC AGGCAAGCCT
GGGCCTTCTG GTCAACCTGG CCGTCCGGGG CCCCCAGGCC CCCCACCTGC AGGACAACTT
ATAATGGGAC CCAAAGGGGA AAGAGGATTT CCCGGGCCTC CAGGAAGATG TCTTTGTGGA
CCCACTATGA ATGTGAATAA CCCTTCCTAC GGGGAATCTG TGTATGGGCC CAGTTCCCCG
CGAGTTCCTG TGATTTTTGT GGTCAACAAC CAGGAGGAGC TTGAGAGGCT GAACACCCAA
AACGCCATTG CCTTCCGCAG AGACCAGAGA TCTCTGTACT TCAAGGACAG CCTTGGCTGG
CTCCCCATCC AGCTGACCCC TTTCTACCCT GTGGATTACA CTGCAGACCA GCACGGCACC
TGTGGGGATG GGCTCCTGCA GCCTGGGGAG GAGTGTGACG ACGGTAACAG CGATGTGGGT
GACGACTGCA TCCGCTGTCA CCGTGCCTAC TGTGGAGATG GTCACCGGCA TGAGGGTGTG
GAGGACTGTG ACGGCTCTGA CTTTGGCTAC CTGACATGCG AGACCTATCT CCCTGGGTCA
TATGGAGACC TGCAATGCAC CCAGTACTGC TACATCGACT CCACGCCCTG CCGCTACTTC
ACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_536800.2
CDS127..1392
Misc Feature(1)97..99(+)
Misc Feature(2)340..564(+)
Misc Feature(3)535..711(+)
Misc Feature(4)1141..>1215(+)
Misc Feature(5)1147..1215(+)
Exon (1)1..232
Gene:COLQ
Gene Synonym:
Exon (2)233..345
Gene:COLQ
Gene Synonym:
Exon (3)346..390
Gene:COLQ
Gene Synonym:
Exon (4)391..417
Gene:COLQ
Gene Synonym:
Exon (5)418..489
Gene:COLQ
Gene Synonym:
Exon (6)490..552
Gene:COLQ
Gene Synonym:
Exon (7)553..579
Gene:COLQ
Gene Synonym:
Exon (8)580..624
Gene:COLQ
Gene Synonym:
Exon (9)625..660
Gene:COLQ
Gene Synonym:
Exon (10)661..741
Gene:COLQ
Gene Synonym:
Exon (11)742..838
Gene:COLQ
Gene Synonym:
Exon (12)839..978
Gene:COLQ
Gene Synonym:
Exon (13)979..1098
Gene:COLQ
Gene Synonym:
Exon (14)1099..1219
Gene:COLQ
Gene Synonym:
Exon (15)1220..1322
Gene:COLQ
Gene Synonym:
Exon (16)1323..2903
Gene:COLQ
Gene Synonym:
Translation
Position Chain Variation Link
2 2 a, g dbSNP:370901328
4 4 -, ac dbSNP:199925079
4 4 a, g dbSNP:201450724
5 5 -, acacac, gc dbSNP:773994272
6 6 -, gc dbSNP:530131087
6 6 a, g dbSNP:113510832
20 20 -, ca dbSNP:767022435
21 21 -, ca dbSNP:746566925
22 22 -, ca dbSNP:3836381
23 23 a, c dbSNP:772196480
31 31 c, t dbSNP:151131743
32 32 a, g dbSNP:555832171
34 34 a, t dbSNP:539193639
36 36 a, g, t dbSNP:567714486
51 51 a, g dbSNP:558496070
76 76 g, t dbSNP:745770526
81 81 a, g dbSNP:73033051
84 84 a, g dbSNP:762115269
86 86 a, g dbSNP:770843659
88 88 c, t dbSNP:748437180
89 89 c, g dbSNP:781577326
92 92 c, t dbSNP:755160062
96 96 a, c dbSNP:747085828
105 105 a, c dbSNP:780128889
106 106 c, g dbSNP:758875066
109 109 c, t dbSNP:750894854
110 110 a, g dbSNP:568283420
122 122 a, c dbSNP:757606818
127 127 a, g dbSNP:754147251
136 136 c, t dbSNP:763742063
138 138 g, t dbSNP:760335356
139 139 a, c dbSNP:372693142
145 145 a, g dbSNP:774887446
147 147 a, g dbSNP:767033386
149 149 c, g dbSNP:189427175
151 151 c, t dbSNP:774415805
163 163 c, g dbSNP:770933718
169 169 c, t dbSNP:762844921
180 180 -, ctct dbSNP:771879602
183 183 -, t dbSNP:777102590
186 186 c, t dbSNP:773097270
187 187 a, g dbSNP:142115188
194 194 a, g dbSNP:563740749
197 197 c, t dbSNP:374642884
198 198 a, g, t dbSNP:111339593
213 213 c, t dbSNP:779409635
214 214 a, g dbSNP:201234648
222 222 a, g dbSNP:757698575
223 223 a, g dbSNP:375205921
230 230 c, t dbSNP:778150901
235 235 c, t dbSNP:753693817
243 243 a, c dbSNP:138891500
246 246 a, g dbSNP:759795366
247 247 a, g dbSNP:774553533
258 258 c, g dbSNP:532869144
259 259 c, t dbSNP:763096476
260 260 a, c, g dbSNP:761717418
261 261 c, t dbSNP:551550075
263 263 g, t dbSNP:191657758
267 267 c, t dbSNP:777090135
268 268 -, c dbSNP:761285078
268 268 c, t dbSNP:769136046
278 278 a, g dbSNP:746714301
281 281 a, g dbSNP:564244822
282 282 c, t dbSNP:758060872
285 285 a, g dbSNP:745419074
288 288 a, g dbSNP:778242187
290 290 a, c, t dbSNP:774271810
291 291 a, g dbSNP:777613214
292 292 c, t dbSNP:755892125
293 293 c, t dbSNP:768517440
297 297 -, tcc dbSNP:753308023
299 299 c, t dbSNP:766617611
301 301 c, t dbSNP:763036328
306 306 a, g dbSNP:750564606
307 307 c, t dbSNP:765195642
313 313 a, c dbSNP:762446165
317 317 c, t dbSNP:188737921
320 320 c, t dbSNP:777261321
331 331 a, g dbSNP:769226066
335 335 g, t dbSNP:761127569
338 338 a, g dbSNP:775959815
340 340 a, g dbSNP:771877645
344 344 c, t dbSNP:374700344
353 353 c, t dbSNP:776165035
361 361 a, c dbSNP:768502040
365 365 g, t dbSNP:746943756
380 380 a, t dbSNP:775189965
381 381 a, g dbSNP:771988325
392 392 c, g dbSNP:781191924
401 401 -, g dbSNP:759874172
403 403 c, g, t dbSNP:143766249
404 404 a, g dbSNP:201856842
412 412 a, g dbSNP:758861218
413 413 a, g dbSNP:750806839
415 415 a, g dbSNP:142980906
422 422 g, t dbSNP:755030467
424 424 c, t dbSNP:751526069
426 426 c, t dbSNP:766356664
429 429 c, t dbSNP:762810817
432 432 c, g dbSNP:772842453
447 447 a, c dbSNP:764397858
450 450 g, t dbSNP:575929335
452 452 a, c dbSNP:373840796
453 453 c, t dbSNP:775589975
462 462 c, t dbSNP:757867510
463 463 a, g dbSNP:370976269
464 464 a, g dbSNP:774853684
474 474 c, t dbSNP:771279997
475 475 a, c dbSNP:114544830
483 483 a, c dbSNP:777953780
484 484 c, t dbSNP:755848079
498 498 -, a dbSNP:770006712
498 498 a, g dbSNP:770038933
500 500 c, g dbSNP:748461738
502 502 a, g dbSNP:780992593
505 505 a, c dbSNP:768382539
508 508 g, t dbSNP:376385207
516 516 a, g dbSNP:779407765
530 530 c, g dbSNP:104893734
541 541 a, g dbSNP:750324839
546 546 c, t dbSNP:113843907
547 547 c, t dbSNP:757056994
557 557 a, c dbSNP:751714432
563 563 g, t dbSNP:766563349
577 577 a, g dbSNP:144705312
579 579 a, g dbSNP:750472473
584 584 c, g dbSNP:201850242
585 585 c, t dbSNP:149370622
587 587 c, g dbSNP:757905303
593 593 a, g dbSNP:377364923
608 608 a, g dbSNP:764640385
622 622 a, g dbSNP:760952573
624 624 a, g dbSNP:775885011
625 625 a, g dbSNP:769665618
630 630 c, t dbSNP:267599659
634 634 a, g dbSNP:374783562
638 638 a, t dbSNP:776357105
645 645 a, c dbSNP:138830715
646 646 a, g dbSNP:746744618
664 664 g, t dbSNP:104893733
667 667 a, g dbSNP:780605254
675 675 a, g dbSNP:777785311
676 676 a, g dbSNP:769908780
678 678 a, g dbSNP:112673051
693 693 a, c dbSNP:781736167
694 694 g, t dbSNP:755424266
698 698 a, c, g dbSNP:780362486
703 703 c, g, t dbSNP:770045897
704 704 a, g dbSNP:764737302
711 711 c, t dbSNP:756797522
718 718 a, g dbSNP:753247779
730 730 c, g, t dbSNP:760669036
731 731 a, g dbSNP:200770910
734 734 a, g dbSNP:767419139
735 735 a, c dbSNP:763329693
737 737 a, g dbSNP:79655731
742 742 g, t dbSNP:104893735
747 747 a, g dbSNP:755703073
755 755 a, g dbSNP:752192353
761 761 c, g dbSNP:146687198
765 765 c, t dbSNP:754845086
773 773 c, t dbSNP:200479277
783 783 a, g dbSNP:373597557
791 791 c, t dbSNP:762151906
793 793 g, t dbSNP:754017581
807 807 c, g dbSNP:745798750
809 809 a, g dbSNP:201158622
810 810 c, t dbSNP:760774340
812 812 -, c dbSNP:759911990
812 812 c, t dbSNP:146619514
813 813 a, g dbSNP:150061580
822 822 a, t dbSNP:759931610
824 824 g, t dbSNP:774848044
826 826 a, c dbSNP:771189959
832 832 c, t dbSNP:749610650
835 835 g, t dbSNP:777577778
836 836 c, t dbSNP:373735085
844 844 c, t dbSNP:763262836
847 847 a, g dbSNP:773686063
859 859 a, c, g dbSNP:114896510
863 863 c, g dbSNP:778156526
868 868 a, t dbSNP:121908922
877 877 c, g dbSNP:746460292
879 879 a, c, t dbSNP:758307097
880 880 a, c, g dbSNP:200403785
882 882 g, t dbSNP:756396914
893 893 a, g dbSNP:752905204
895 895 g, t dbSNP:374490483
913 913 a, g dbSNP:755020019
928 928 c, g dbSNP:751621776
933 933 c, t dbSNP:766821521
936 936 -, c dbSNP:763209840
938 938 g, t dbSNP:763518486
942 942 a, g, t dbSNP:765680430
946 946 a, g dbSNP:184245561
949 949 -, t dbSNP:773636951
950 950 a, g dbSNP:200232229
952 952 a, g dbSNP:776425306
958 958 a, c, g dbSNP:6782980
961 961 a, t dbSNP:143918191
962 962 c, g dbSNP:550979119
965 965 c, t dbSNP:370201862
966 966 a, g dbSNP:771944316
967 967 c, t dbSNP:121908924
968 968 a, g dbSNP:778740405
991 991 a, t dbSNP:369551954
993 993 a, c dbSNP:760343679
994 994 a, c dbSNP:201036462
1002 1002 a, g dbSNP:113488563
1006 1006 a, c dbSNP:771559323
1011 1011 c, g dbSNP:758991725
1023 1023 a, c dbSNP:774279832
1024 1024 c, g dbSNP:113708721
1029 1029 c, t dbSNP:770819352
1041 1041 a, c dbSNP:749107627
1042 1042 c, t dbSNP:138818971
1043 1043 a, g dbSNP:375225755
1048 1048 a, g dbSNP:184944796
1050 1050 a, c, g dbSNP:758554049
1055 1055 a, g dbSNP:149532541
1060 1060 a, c dbSNP:750489224
1061 1061 c, t dbSNP:779583406
1063 1063 c, t dbSNP:757815714
1082 1082 c, g dbSNP:754194997
1086 1086 g, t dbSNP:764516134
1091 1091 c, g dbSNP:760303798
1093 1093 a, g dbSNP:752410020
1096 1096 c, g dbSNP:372064982
1103 1103 c, t dbSNP:779104097
1105 1105 c, t dbSNP:116828761
1106 1106 -, c dbSNP:769982050
1109 1109 c, t dbSNP:754357969
1110 1110 c, g dbSNP:778085094
1111 1111 c, t dbSNP:756508281
1113 1113 c, g dbSNP:752967536
1132 1132 a, g, t dbSNP:116373583
1140 1140 c, t dbSNP:759169923
1141 1141 a, g dbSNP:202019416
1152 1152 g, t dbSNP:751113924
1154 1154 a, g dbSNP:765874335
1171 1171 a, g dbSNP:762306354
1177 1177 c, g dbSNP:140955551
1179 1179 a, c, g dbSNP:116503231
1181 1181 c, g dbSNP:368575417
1185 1185 c, t dbSNP:539100854
1188 1188 c, t dbSNP:149852377
1189 1189 a, g dbSNP:774638847
1197 1197 c, t dbSNP:771047223
1198 1198 a, g dbSNP:749266800
1209 1209 c, t dbSNP:548322139
1210 1210 a, c, g dbSNP:748582765
1218 1218 c, t dbSNP:199930637
1219 1219 c, t dbSNP:779594053
1220 1220 a, g dbSNP:375272767
1229 1229 a, g dbSNP:140670616
1233 1233 c, t dbSNP:753830857
1235 1235 a, g dbSNP:764064240
1236 1236 -, c dbSNP:771866680
1236 1236 c, t dbSNP:760567044
1244 1244 a, g dbSNP:752498913
1252 1252 a, c, t dbSNP:139574075
1257 1257 a, t dbSNP:773394999
1261 1261 a, g dbSNP:769772442
1272 1272 c, t dbSNP:55866379
1278 1278 c, t dbSNP:777147864
1279 1279 a, g dbSNP:769114264
1298 1298 a, t dbSNP:753690228
1301 1301 a, c, t dbSNP:536042715
1302 1302 a, g, t dbSNP:745502271
1305 1305 c, t dbSNP:185829251
1306 1306 a, g dbSNP:141721093
1310 1310 a, c dbSNP:550616958
1312 1312 c, t dbSNP:753348773
1313 1313 a, c dbSNP:121908923
1319 1319 c, t dbSNP:756084974
1323 1323 g, t dbSNP:754755194
1341 1341 a, g dbSNP:369122841
1345 1345 a, g, t dbSNP:375215281
1347 1347 c, g dbSNP:753978763
1358 1358 a, g dbSNP:764155601
1360 1360 a, c dbSNP:371867135
1362 1362 a, c, g, t dbSNP:73818504
1363 1363 a, g, t dbSNP:770753693
1368 1368 c, g dbSNP:748932004
1369 1369 a, c dbSNP:772919447
1370 1370 -, c dbSNP:757053860
1371 1371 a, g dbSNP:769428838
1374 1374 c, t dbSNP:747648795
1378 1378 c, t dbSNP:368932156
1379 1379 a, g dbSNP:754915644
1383 1383 c, t dbSNP:746832080
1390 1390 c, t dbSNP:188841362
1396 1396 -, t dbSNP:748930865
1396 1396 a, c, t dbSNP:754063500
1397 1397 c, t dbSNP:753583108
1398 1398 a, g, t dbSNP:144621591
1409 1409 a, g dbSNP:142437859
1411 1411 a, g dbSNP:760065319
1412 1412 a, g dbSNP:766337085
1416 1416 a, g dbSNP:774910654
1417 1417 c, t dbSNP:766842695
1431 1431 a, g dbSNP:763339889
1435 1435 g, t dbSNP:773025427
1436 1436 c, t dbSNP:769535072
1451 1451 c, t dbSNP:747654042
1452 1452 a, g dbSNP:776185686
1477 1477 a, g dbSNP:768534998
1482 1482 c, t dbSNP:746964365
1487 1487 a, t dbSNP:779900149
1491 1491 c, t dbSNP:758203590
1495 1495 a, g dbSNP:745613708
1497 1497 a, g dbSNP:761993988
1506 1506 a, g dbSNP:778002170
1516 1516 a, t dbSNP:184812820
1526 1526 a, g dbSNP:752767490
1536 1536 c, g dbSNP:375831415
1540 1540 -, actca dbSNP:777457693
1546 1546 g, t dbSNP:755501265
1548 1548 c, t dbSNP:752112752
1561 1561 a, t dbSNP:550256310
1581 1581 c, t dbSNP:749985637
1602 1602 -, aa dbSNP:138019222
1604 1604 c, t dbSNP:530125773
1627 1627 c, t dbSNP:537145282
1638 1638 c, t dbSNP:374251957
1639 1639 a, g dbSNP:551512511
1668 1668 a, g dbSNP:766923758
1679 1679 c, t dbSNP:769285503
1690 1690 c, t dbSNP:144067075
1705 1705 a, g dbSNP:565997789
1711 1711 c, t dbSNP:549636072
1736 1736 c, t dbSNP:2278961
1757 1757 a, t dbSNP:2278962
1788 1788 a, c dbSNP:372672661
1799 1799 c, t dbSNP:569980572
1800 1800 a, g dbSNP:771628214
1805 1805 -, t dbSNP:534228650
1813 1813 a, g dbSNP:533015053
1814 1814 a, g dbSNP:564176947
1818 1818 a, g dbSNP:763440701
1856 1856 a, g, t dbSNP:770219393
1883 1883 a, g dbSNP:541202497
1884 1884 a, g dbSNP:116231717
1896 1896 a, g dbSNP:746360529
1907 1907 c, t dbSNP:10154896
1908 1908 a, g dbSNP:544927968
1942 1942 c, t dbSNP:576307598
1946 1946 c, t dbSNP:557314953
1947 1947 g, t dbSNP:116450586
1975 1975 a, g dbSNP:747056584
1987 1987 -, g dbSNP:149076823
2095 2095 c, g dbSNP:537057072
2096 2096 a, g dbSNP:577687841
2143 2143 c, t dbSNP:368981773
2198 2198 c, t dbSNP:534825077
2240 2240 c, t dbSNP:550045499
2296 2296 c, t dbSNP:181965246
2318 2318 c, g dbSNP:536136335
2337 2337 a, g dbSNP:372867726
2369 2369 c, t dbSNP:549747763
2386 2386 a, g dbSNP:77521642
2390 2390 c, t dbSNP:570530635
2393 2393 a, g dbSNP:3846128
2425 2425 c, t dbSNP:771512608
2460 2460 a, g dbSNP:527411340
2465 2465 a, g dbSNP:561918872
2469 2469 a, g dbSNP:541699261
2475 2475 c, t dbSNP:749123750
2476 2476 a, g dbSNP:376202299
2570 2570 c, t dbSNP:747653111
2640 2640 c, t dbSNP:373209743
2663 2663 a, g dbSNP:779804779
2669 2669 c, t dbSNP:572408115
2672 2672 c, t dbSNP:146270961
2723 2723 c, g dbSNP:756102599
2725 2725 c, t dbSNP:778433921
2726 2726 a, g dbSNP:543377033
2739 2739 c, t dbSNP:189542753
2750 2750 c, t dbSNP:757814018
2758 2758 a, g dbSNP:376129145
2778 2778 a, g dbSNP:376193770
2787 2787 c, t dbSNP:3274
2790 2790 c, g dbSNP:756739237
2799 2799 a, g dbSNP:113737086
2882 2882 a, c dbSNP:751038602

Target ORF information:

RefSeq Version NM_080539
Organism Homo sapiens (human)
Definition Homo sapiens collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant III, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_080539

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGTTGTCC TGAATCCAAT GACTTTGGGA ATTTATCTTC AGCTTTTCTT CCTCTCTATC 
GTGTCTCAGC CGACTTTCAT CAACAGCGTT CTTCCAATCT CAGCAGCCCT TCCCAGCCTG
GATCAGAAGA AGCGTGGTGG CCACAAAGCA TGCTGCCTGC TGACGCCTCC TCCACCACCA
CTGTTCCCAC CACCATTCTT CAGAGGTGGC CGAAGTCCGG GTCCACCGGG GCTTCCTGGC
AAGACAGGAC CAAAGGGAGA AAAGGGGGAG CTTGGCCGAC CAGGAAGGAA GGGTAGACCT
GGCCCCCCAG GTGTTCCTGG CATGCCTGGG CCCATCGGTT GGCCAGGCCC TGAAGGACCC
AGGGGTGAAA AAGGTGACCT GGGTATGATG GGCTTGCCAG GGTCAAGAGG ACCAATGGGC
TCCAAGGGCT ACCCTGGATC CAGAGGGGAA AAGGGATCCA GAGGTGAAAA GGGTGACCTG
GGTCCCAAAG GAGAAAAGGG TTTCCCAGGA TTTCCTGGAA TGTTGGGGCA GAAAGGTGAA
ATGGGTCCAA AAGGTGAACC TGGGATAGCA GGACACCGAG GACCCACAGG AAGACCAGGA
AAACGAGGCA AGCAGGGACA GAAAGGGGAT AGTGGAGTTA TGGGCCCACC AGGCAAGCCT
GGGCCTTCTG GTCAACCTGG CCGTCCGGGG CCCCCAGGCC CCCCACCTGC AGGACAACTT
ATAATGGGAC CCAAAGGGGA AAGAGGATTT CCCGGGCCTC CAGGAAGATG TCTTTGTGGA
CCCACTATGA ATGTGAATAA CCCTTCCTAC GGGGAATCTG TGTATGGGCC CAGTTCCCCG
CGAGTTCCTG TGATTTTTGT GGTCAACAAC CAGGAGGAGC TTGAGAGGCT GAACACCCAA
AACGCCATTG CCTTCCGCAG AGACCAGAGA TCTCTGTACT TCAAGGACAG CCTTGGCTGG
CTCCCCATCC AGCTGACCCC TTTCTACCCT GTGGATTACA CTGCAGACCA GCACGGCACC
TGTGGGGATG GGCTCCTGCA GCCTGGGGAG GAGTGTGACG ACGGTAACAG CGATGTGGGT
GACGACTGCA TCCGCTGTCA CCGTGCCTAC TGTGGAGATG GTCACCGGCA TGAGGGTGTG
GAGGACTGTG ACGGCTCTGA CTTTGGCTAC CTGACATGCG AGACCTATCT CCCTGGGTCA
TATGGAGACC TGCAATGCAC CCAGTACTGC TACATCGACT CCACGCCCTG CCGCTACTTC
ACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19054
Accession Version NM_005677.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1368bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product acetylcholinesterase collagenic tail peptide isoform I precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF057036.1, AK128401.1 and AI554171.1. This sequence is a reference standard in the RefSeqGene project. On Oct 27, 2006 this sequence version replaced gi:18105015. Summary: This gene encodes the subunit of a collagen-like molecule associated with acetylcholinesterase in skeletal muscle. Each molecule is composed of three identical subunits. Each subunit contains a proline-rich attachment domain (PRAD) that binds an acetylcholinesterase tetramer to anchor the catalytic subunit of the enzyme to the basal lamina. Mutations in this gene are associated with endplate acetylcholinesterase deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (I) encodes the longest protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF057036.1, AK289688.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTTGTCC TGAATCCAAT GACTTTGGGA ATTTATCTTC AGCTTTTCTT CCTCTCTATC 
GTGTCTCAGC CGACTTTCAT CAACAGCGTT CTTCCAATCT CAGCAGCCCT TCCCAGCCTG
GATCAGAAGA AGCGTGGTGG CCACAAAGCA TGCTGCCTGC TGACGCCTCC TCCACCACCA
CTGTTCCCAC CACCATTCTT CAGAGGTGGC CGAAGTCCGC TTCTCTCCCC AGACATGAAG
AATCTCATGC TGGAACTGGA GACCTCGCAG TCCCCGTGCA TGCAAGGCTC GCTAGGCTCC
CCTGGGCCTC CCGGCCCCCA GGGTCCACCG GGGCTTCCTG GCAAGACAGG ACCAAAGGGA
GAAAAGGGGG AGCTTGGCCG ACCAGGAAGG AAGGGTAGAC CTGGCCCCCC AGGTGTTCCT
GGCATGCCTG GGCCCATCGG TTGGCCAGGC CCTGAAGGAC CCAGGGGTGA AAAAGGTGAC
CTGGGTATGA TGGGCTTGCC AGGGTCAAGA GGACCAATGG GCTCCAAGGG CTACCCTGGA
TCCAGAGGGG AAAAGGGATC CAGAGGTGAA AAGGGTGACC TGGGTCCCAA AGGAGAAAAG
GGTTTCCCAG GATTTCCTGG AATGTTGGGG CAGAAAGGTG AAATGGGTCC AAAAGGTGAA
CCTGGGATAG CAGGACACCG AGGACCCACA GGAAGACCAG GAAAACGAGG CAAGCAGGGA
CAGAAAGGGG ATAGTGGAGT TATGGGCCCA CCAGGCAAGC CTGGGCCTTC TGGTCAACCT
GGCCGTCCGG GGCCCCCAGG CCCCCCACCT GCAGGACAAC TTATAATGGG ACCCAAAGGG
GAAAGAGGAT TTCCCGGGCC TCCAGGAAGA TGTCTTTGTG GACCCACTAT GAATGTGAAT
AACCCTTCCT ACGGGGAATC TGTGTATGGG CCCAGTTCCC CGCGAGTTCC TGTGATTTTT
GTGGTCAACA ACCAGGAGGA GCTTGAGAGG CTGAACACCC AAAACGCCAT TGCCTTCCGC
AGAGACCAGA GATCTCTGTA CTTCAAGGAC AGCCTTGGCT GGCTCCCCAT CCAGCTGACC
CCTTTCTACC CTGTGGATTA CACTGCAGAC CAGCACGGCA CCTGTGGGGA TGGGCTCCTG
CAGCCTGGGG AGGAGTGTGA CGACGGTAAC AGCGATGTGG GTGACGACTG CATCCGCTGT
CACCGTGCCT ACTGTGGAGA TGGTCACCGG CATGAGGGTG TGGAGGACTG TGACGGCTCT
GACTTTGGCT ACCTGACATG CGAGACCTAT CTCCCTGGGT CATATGGAGA CCTGCAATGC
ACCCAGTACT GCTACATCGA CTCCACGCCC TGCCGCTACT TCACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_005668.2
CDS127..1494
Misc Feature(1)97..99(+)
Misc Feature(2)277..327(+)
Misc Feature(3)514..525(+)
Misc Feature(4)637..813(+)
Misc Feature(5)829..840(+)
Misc Feature(6)1243..>1317(+)
Misc Feature(7)1249..1317(+)
Exon (1)1..232
Gene:COLQ
Gene Synonym:
Exon (2)233..345
Gene:COLQ
Gene Synonym:
Exon (3)346..447
Gene:COLQ
Gene Synonym:
Exon (4)448..492
Gene:COLQ
Gene Synonym:
Exon (5)493..519
Gene:COLQ
Gene Synonym:
Exon (6)520..591
Gene:COLQ
Gene Synonym:
Exon (7)592..654
Gene:COLQ
Gene Synonym:
Exon (8)655..681
Gene:COLQ
Gene Synonym:
Exon (9)682..726
Gene:COLQ
Gene Synonym:
Exon (10)727..762
Gene:COLQ
Gene Synonym:
Exon (11)763..843
Gene:COLQ
Gene Synonym:
Exon (12)844..940
Gene:COLQ
Gene Synonym:
Exon (13)941..1080
Gene:COLQ
Gene Synonym:
Exon (14)1081..1200
Gene:COLQ
Gene Synonym:
Exon (15)1201..1321
Gene:COLQ
Gene Synonym:
Exon (16)1322..1424
Gene:COLQ
Gene Synonym:
Exon (17)1425..3005
Gene:COLQ
Gene Synonym:
Translation
Position Chain Variation Link
2 2 a, g dbSNP:370901328
4 4 -, ac dbSNP:199925079
4 4 a, g dbSNP:201450724
5 5 -, acacac, gc dbSNP:773994272
6 6 -, gc dbSNP:530131087
6 6 a, g dbSNP:113510832
20 20 -, ca dbSNP:767022435
21 21 -, ca dbSNP:746566925
22 22 -, ca dbSNP:3836381
23 23 a, c dbSNP:772196480
31 31 c, t dbSNP:151131743
32 32 a, g dbSNP:555832171
34 34 a, t dbSNP:539193639
36 36 a, g, t dbSNP:567714486
51 51 a, g dbSNP:558496070
76 76 g, t dbSNP:745770526
81 81 a, g dbSNP:73033051
84 84 a, g dbSNP:762115269
86 86 a, g dbSNP:770843659
88 88 c, t dbSNP:748437180
89 89 c, g dbSNP:781577326
92 92 c, t dbSNP:755160062
96 96 a, c dbSNP:747085828
105 105 a, c dbSNP:780128889
106 106 c, g dbSNP:758875066
109 109 c, t dbSNP:750894854
110 110 a, g dbSNP:568283420
122 122 a, c dbSNP:757606818
127 127 a, g dbSNP:754147251
136 136 c, t dbSNP:763742063
138 138 g, t dbSNP:760335356
139 139 a, c dbSNP:372693142
145 145 a, g dbSNP:774887446
147 147 a, g dbSNP:767033386
149 149 c, g dbSNP:189427175
151 151 c, t dbSNP:774415805
163 163 c, g dbSNP:770933718
169 169 c, t dbSNP:762844921
180 180 -, ctct dbSNP:771879602
183 183 -, t dbSNP:777102590
186 186 c, t dbSNP:773097270
187 187 a, g dbSNP:142115188
194 194 a, g dbSNP:563740749
197 197 c, t dbSNP:374642884
198 198 a, g, t dbSNP:111339593
213 213 c, t dbSNP:779409635
214 214 a, g dbSNP:201234648
222 222 a, g dbSNP:757698575
223 223 a, g dbSNP:375205921
230 230 c, t dbSNP:778150901
235 235 c, t dbSNP:753693817
243 243 a, c dbSNP:138891500
246 246 a, g dbSNP:759795366
247 247 a, g dbSNP:774553533
258 258 c, g dbSNP:532869144
259 259 c, t dbSNP:763096476
260 260 a, c, g dbSNP:761717418
261 261 c, t dbSNP:551550075
263 263 g, t dbSNP:191657758
267 267 c, t dbSNP:777090135
268 268 -, c dbSNP:761285078
268 268 c, t dbSNP:769136046
278 278 a, g dbSNP:746714301
281 281 a, g dbSNP:564244822
282 282 c, t dbSNP:758060872
285 285 a, g dbSNP:745419074
288 288 a, g dbSNP:778242187
290 290 a, c, t dbSNP:774271810
291 291 a, g dbSNP:777613214
292 292 c, t dbSNP:755892125
293 293 c, t dbSNP:768517440
297 297 -, tcc dbSNP:753308023
299 299 c, t dbSNP:766617611
301 301 c, t dbSNP:763036328
306 306 a, g dbSNP:750564606
307 307 c, t dbSNP:765195642
313 313 a, c dbSNP:762446165
317 317 c, t dbSNP:188737921
320 320 c, t dbSNP:777261321
331 331 a, g dbSNP:769226066
335 335 g, t dbSNP:761127569
338 338 a, g dbSNP:775959815
340 340 a, g dbSNP:771877645
344 344 c, t dbSNP:374700344
359 359 a, t dbSNP:762838086
361 361 a, g dbSNP:770561043
367 367 a, g dbSNP:748826424
368 368 a, g dbSNP:777215134
372 372 c, t dbSNP:372432709
375 375 a, g dbSNP:748046000
376 376 c, t dbSNP:369308750
383 383 c, t dbSNP:754712383
389 389 c, t dbSNP:746757636
393 393 a, g dbSNP:144897863
401 401 c, t dbSNP:757464366
402 402 a, g dbSNP:753997328
408 408 a, g dbSNP:764250328
413 413 g, t dbSNP:369852257
414 414 c, t dbSNP:756730529
416 416 c, t dbSNP:531584926
417 417 a, g, t dbSNP:115201284
426 426 c, t dbSNP:2305611
439 439 a, g dbSNP:201507876
455 455 c, t dbSNP:776165035
463 463 a, c dbSNP:768502040
467 467 g, t dbSNP:746943756
482 482 a, t dbSNP:775189965
483 483 a, g dbSNP:771988325
494 494 c, g dbSNP:781191924
503 503 -, g dbSNP:759874172
505 505 c, g, t dbSNP:143766249
506 506 a, g dbSNP:201856842
514 514 a, g dbSNP:758861218
515 515 a, g dbSNP:750806839
517 517 a, g dbSNP:142980906
524 524 g, t dbSNP:755030467
526 526 c, t dbSNP:751526069
528 528 c, t dbSNP:766356664
531 531 c, t dbSNP:762810817
534 534 c, g dbSNP:772842453
549 549 a, c dbSNP:764397858
552 552 g, t dbSNP:575929335
554 554 a, c dbSNP:373840796
555 555 c, t dbSNP:775589975
564 564 c, t dbSNP:757867510
565 565 a, g dbSNP:370976269
566 566 a, g dbSNP:774853684
576 576 c, t dbSNP:771279997
577 577 a, c dbSNP:114544830
585 585 a, c dbSNP:777953780
586 586 c, t dbSNP:755848079
600 600 -, a dbSNP:770006712
600 600 a, g dbSNP:770038933
602 602 c, g dbSNP:748461738
604 604 a, g dbSNP:780992593
607 607 a, c dbSNP:768382539
610 610 g, t dbSNP:376385207
618 618 a, g dbSNP:779407765
632 632 c, g dbSNP:104893734
643 643 a, g dbSNP:750324839
648 648 c, t dbSNP:113843907
649 649 c, t dbSNP:757056994
659 659 a, c dbSNP:751714432
665 665 g, t dbSNP:766563349
679 679 a, g dbSNP:144705312
681 681 a, g dbSNP:750472473
686 686 c, g dbSNP:201850242
687 687 c, t dbSNP:149370622
689 689 c, g dbSNP:757905303
695 695 a, g dbSNP:377364923
710 710 a, g dbSNP:764640385
724 724 a, g dbSNP:760952573
726 726 a, g dbSNP:775885011
727 727 a, g dbSNP:769665618
732 732 c, t dbSNP:267599659
736 736 a, g dbSNP:374783562
740 740 a, t dbSNP:776357105
747 747 a, c dbSNP:138830715
748 748 a, g dbSNP:746744618
766 766 g, t dbSNP:104893733
769 769 a, g dbSNP:780605254
777 777 a, g dbSNP:777785311
778 778 a, g dbSNP:769908780
780 780 a, g dbSNP:112673051
795 795 a, c dbSNP:781736167
796 796 g, t dbSNP:755424266
800 800 a, c, g dbSNP:780362486
805 805 c, g, t dbSNP:770045897
806 806 a, g dbSNP:764737302
813 813 c, t dbSNP:756797522
820 820 a, g dbSNP:753247779
832 832 c, g, t dbSNP:760669036
833 833 a, g dbSNP:200770910
836 836 a, g dbSNP:767419139
837 837 a, c dbSNP:763329693
839 839 a, g dbSNP:79655731
844 844 g, t dbSNP:104893735
849 849 a, g dbSNP:755703073
857 857 a, g dbSNP:752192353
863 863 c, g dbSNP:146687198
867 867 c, t dbSNP:754845086
875 875 c, t dbSNP:200479277
885 885 a, g dbSNP:373597557
893 893 c, t dbSNP:762151906
895 895 g, t dbSNP:754017581
909 909 c, g dbSNP:745798750
911 911 a, g dbSNP:201158622
912 912 c, t dbSNP:760774340
914 914 -, c dbSNP:759911990
914 914 c, t dbSNP:146619514
915 915 a, g dbSNP:150061580
924 924 a, t dbSNP:759931610
926 926 g, t dbSNP:774848044
928 928 a, c dbSNP:771189959
934 934 c, t dbSNP:749610650
937 937 g, t dbSNP:777577778
938 938 c, t dbSNP:373735085
946 946 c, t dbSNP:763262836
949 949 a, g dbSNP:773686063
961 961 a, c, g dbSNP:114896510
965 965 c, g dbSNP:778156526
970 970 a, t dbSNP:121908922
979 979 c, g dbSNP:746460292
981 981 a, c, t dbSNP:758307097
982 982 a, c, g dbSNP:200403785
984 984 g, t dbSNP:756396914
995 995 a, g dbSNP:752905204
997 997 g, t dbSNP:374490483
1015 1015 a, g dbSNP:755020019
1030 1030 c, g dbSNP:751621776
1035 1035 c, t dbSNP:766821521
1038 1038 -, c dbSNP:763209840
1040 1040 g, t dbSNP:763518486
1044 1044 a, g, t dbSNP:765680430
1048 1048 a, g dbSNP:184245561
1051 1051 -, t dbSNP:773636951
1052 1052 a, g dbSNP:200232229
1054 1054 a, g dbSNP:776425306
1060 1060 a, c, g dbSNP:6782980
1063 1063 a, t dbSNP:143918191
1064 1064 c, g dbSNP:550979119
1067 1067 c, t dbSNP:370201862
1068 1068 a, g dbSNP:771944316
1069 1069 c, t dbSNP:121908924
1070 1070 a, g dbSNP:778740405
1093 1093 a, t dbSNP:369551954
1095 1095 a, c dbSNP:760343679
1096 1096 a, c dbSNP:201036462
1104 1104 a, g dbSNP:113488563
1108 1108 a, c dbSNP:771559323
1113 1113 c, g dbSNP:758991725
1125 1125 a, c dbSNP:774279832
1126 1126 c, g dbSNP:113708721
1131 1131 c, t dbSNP:770819352
1143 1143 a, c dbSNP:749107627
1144 1144 c, t dbSNP:138818971
1145 1145 a, g dbSNP:375225755
1150 1150 a, g dbSNP:184944796
1152 1152 a, c, g dbSNP:758554049
1157 1157 a, g dbSNP:149532541
1162 1162 a, c dbSNP:750489224
1163 1163 c, t dbSNP:779583406
1165 1165 c, t dbSNP:757815714
1184 1184 c, g dbSNP:754194997
1188 1188 g, t dbSNP:764516134
1193 1193 c, g dbSNP:760303798
1195 1195 a, g dbSNP:752410020
1198 1198 c, g dbSNP:372064982
1205 1205 c, t dbSNP:779104097
1207 1207 c, t dbSNP:116828761
1208 1208 -, c dbSNP:769982050
1211 1211 c, t dbSNP:754357969
1212 1212 c, g dbSNP:778085094
1213 1213 c, t dbSNP:756508281
1215 1215 c, g dbSNP:752967536
1234 1234 a, g, t dbSNP:116373583
1242 1242 c, t dbSNP:759169923
1243 1243 a, g dbSNP:202019416
1254 1254 g, t dbSNP:751113924
1256 1256 a, g dbSNP:765874335
1273 1273 a, g dbSNP:762306354
1279 1279 c, g dbSNP:140955551
1281 1281 a, c, g dbSNP:116503231
1283 1283 c, g dbSNP:368575417
1287 1287 c, t dbSNP:539100854
1290 1290 c, t dbSNP:149852377
1291 1291 a, g dbSNP:774638847
1299 1299 c, t dbSNP:771047223
1300 1300 a, g dbSNP:749266800
1311 1311 c, t dbSNP:548322139
1312 1312 a, c, g dbSNP:748582765
1320 1320 c, t dbSNP:199930637
1321 1321 c, t dbSNP:779594053
1322 1322 a, g dbSNP:375272767
1331 1331 a, g dbSNP:140670616
1335 1335 c, t dbSNP:753830857
1337 1337 a, g dbSNP:764064240
1338 1338 -, c dbSNP:771866680
1338 1338 c, t dbSNP:760567044
1346 1346 a, g dbSNP:752498913
1354 1354 a, c, t dbSNP:139574075
1359 1359 a, t dbSNP:773394999
1363 1363 a, g dbSNP:769772442
1374 1374 c, t dbSNP:55866379
1380 1380 c, t dbSNP:777147864
1381 1381 a, g dbSNP:769114264
1400 1400 a, t dbSNP:753690228
1403 1403 a, c, t dbSNP:536042715
1404 1404 a, g, t dbSNP:745502271
1407 1407 c, t dbSNP:185829251
1408 1408 a, g dbSNP:141721093
1412 1412 a, c dbSNP:550616958
1414 1414 c, t dbSNP:753348773
1415 1415 a, c dbSNP:121908923
1421 1421 c, t dbSNP:756084974
1425 1425 g, t dbSNP:754755194
1443 1443 a, g dbSNP:369122841
1447 1447 a, g, t dbSNP:375215281
1449 1449 c, g dbSNP:753978763
1460 1460 a, g dbSNP:764155601
1462 1462 a, c dbSNP:371867135
1464 1464 a, c, g, t dbSNP:73818504
1465 1465 a, g, t dbSNP:770753693
1470 1470 c, g dbSNP:748932004
1471 1471 a, c dbSNP:772919447
1472 1472 -, c dbSNP:757053860
1473 1473 a, g dbSNP:769428838
1476 1476 c, t dbSNP:747648795
1480 1480 c, t dbSNP:368932156
1481 1481 a, g dbSNP:754915644
1485 1485 c, t dbSNP:746832080
1492 1492 c, t dbSNP:188841362
1498 1498 -, t dbSNP:748930865
1498 1498 a, c, t dbSNP:754063500
1499 1499 c, t dbSNP:753583108
1500 1500 a, g, t dbSNP:144621591
1511 1511 a, g dbSNP:142437859
1513 1513 a, g dbSNP:760065319
1514 1514 a, g dbSNP:766337085
1518 1518 a, g dbSNP:774910654
1519 1519 c, t dbSNP:766842695
1533 1533 a, g dbSNP:763339889
1537 1537 g, t dbSNP:773025427
1538 1538 c, t dbSNP:769535072
1553 1553 c, t dbSNP:747654042
1554 1554 a, g dbSNP:776185686
1579 1579 a, g dbSNP:768534998
1584 1584 c, t dbSNP:746964365
1589 1589 a, t dbSNP:779900149
1593 1593 c, t dbSNP:758203590
1597 1597 a, g dbSNP:745613708
1599 1599 a, g dbSNP:761993988
1608 1608 a, g dbSNP:778002170
1618 1618 a, t dbSNP:184812820
1628 1628 a, g dbSNP:752767490
1638 1638 c, g dbSNP:375831415
1642 1642 -, actca dbSNP:777457693
1648 1648 g, t dbSNP:755501265
1650 1650 c, t dbSNP:752112752
1663 1663 a, t dbSNP:550256310
1683 1683 c, t dbSNP:749985637
1704 1704 -, aa dbSNP:138019222
1706 1706 c, t dbSNP:530125773
1729 1729 c, t dbSNP:537145282
1740 1740 c, t dbSNP:374251957
1741 1741 a, g dbSNP:551512511
1770 1770 a, g dbSNP:766923758
1781 1781 c, t dbSNP:769285503
1792 1792 c, t dbSNP:144067075
1807 1807 a, g dbSNP:565997789
1813 1813 c, t dbSNP:549636072
1838 1838 c, t dbSNP:2278961
1859 1859 a, t dbSNP:2278962
1890 1890 a, c dbSNP:372672661
1901 1901 c, t dbSNP:569980572
1902 1902 a, g dbSNP:771628214
1907 1907 -, t dbSNP:534228650
1915 1915 a, g dbSNP:533015053
1916 1916 a, g dbSNP:564176947
1920 1920 a, g dbSNP:763440701
1958 1958 a, g, t dbSNP:770219393
1985 1985 a, g dbSNP:541202497
1986 1986 a, g dbSNP:116231717
1998 1998 a, g dbSNP:746360529
2009 2009 c, t dbSNP:10154896
2010 2010 a, g dbSNP:544927968
2044 2044 c, t dbSNP:576307598
2048 2048 c, t dbSNP:557314953
2049 2049 g, t dbSNP:116450586
2077 2077 a, g dbSNP:747056584
2089 2089 -, g dbSNP:149076823
2197 2197 c, g dbSNP:537057072
2198 2198 a, g dbSNP:577687841
2245 2245 c, t dbSNP:368981773
2300 2300 c, t dbSNP:534825077
2342 2342 c, t dbSNP:550045499
2398 2398 c, t dbSNP:181965246
2420 2420 c, g dbSNP:536136335
2439 2439 a, g dbSNP:372867726
2471 2471 c, t dbSNP:549747763
2488 2488 a, g dbSNP:77521642
2492 2492 c, t dbSNP:570530635
2495 2495 a, g dbSNP:3846128
2527 2527 c, t dbSNP:771512608
2562 2562 a, g dbSNP:527411340
2567 2567 a, g dbSNP:561918872
2571 2571 a, g dbSNP:541699261
2577 2577 c, t dbSNP:749123750
2578 2578 a, g dbSNP:376202299
2672 2672 c, t dbSNP:747653111
2742 2742 c, t dbSNP:373209743
2765 2765 a, g dbSNP:779804779
2771 2771 c, t dbSNP:572408115
2774 2774 c, t dbSNP:146270961
2825 2825 c, g dbSNP:756102599
2827 2827 c, t dbSNP:778433921
2828 2828 a, g dbSNP:543377033
2841 2841 c, t dbSNP:189542753
2852 2852 c, t dbSNP:757814018
2860 2860 a, g dbSNP:376129145
2880 2880 a, g dbSNP:376193770
2889 2889 c, t dbSNP:3274
2892 2892 c, g dbSNP:756739237
2901 2901 a, g dbSNP:113737086
2984 2984 a, c dbSNP:751038602

Target ORF information:

RefSeq Version NM_005677
Organism Homo sapiens (human)
Definition Homo sapiens collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005677

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTTGTCC TGAATCCAAT GACTTTGGGA ATTTATCTTC AGCTTTTCTT CCTCTCTATC 
GTGTCTCAGC CGACTTTCAT CAACAGCGTT CTTCCAATCT CAGCAGCCCT TCCCAGCCTG
GATCAGAAGA AGCGTGGTGG CCACAAAGCA TGCTGCCTGC TGACGCCTCC TCCACCACCA
CTGTTCCCAC CACCATTCTT CAGAGGTGGC CGAAGTCCGC TTCTCTCCCC AGACATGAAG
AATCTCATGC TGGAACTGGA GACCTCGCAG TCCCCGTGCA TGCAAGGCTC GCTAGGCTCC
CCTGGGCCTC CCGGCCCCCA GGGTCCACCG GGGCTTCCTG GCAAGACAGG ACCAAAGGGA
GAAAAGGGGG AGCTTGGCCG ACCAGGAAGG AAGGGTAGAC CTGGCCCCCC AGGTGTTCCT
GGCATGCCTG GGCCCATCGG TTGGCCAGGC CCTGAAGGAC CCAGGGGTGA AAAAGGTGAC
CTGGGTATGA TGGGCTTGCC AGGGTCAAGA GGACCAATGG GCTCCAAGGG CTACCCTGGA
TCCAGAGGGG AAAAGGGATC CAGAGGTGAA AAGGGTGACC TGGGTCCCAA AGGAGAAAAG
GGTTTCCCAG GATTTCCTGG AATGTTGGGG CAGAAAGGTG AAATGGGTCC AAAAGGTGAA
CCTGGGATAG CAGGACACCG AGGACCCACA GGAAGACCAG GAAAACGAGG CAAGCAGGGA
CAGAAAGGGG ATAGTGGAGT TATGGGCCCA CCAGGCAAGC CTGGGCCTTC TGGTCAACCT
GGCCGTCCGG GGCCCCCAGG CCCCCCACCT GCAGGACAAC TTATAATGGG ACCCAAAGGG
GAAAGAGGAT TTCCCGGGCC TCCAGGAAGA TGTCTTTGTG GACCCACTAT GAATGTGAAT
AACCCTTCCT ACGGGGAATC TGTGTATGGG CCCAGTTCCC CGCGAGTTCC TGTGATTTTT
GTGGTCAACA ACCAGGAGGA GCTTGAGAGG CTGAACACCC AAAACGCCAT TGCCTTCCGC
AGAGACCAGA GATCTCTGTA CTTCAAGGAC AGCCTTGGCT GGCTCCCCAT CCAGCTGACC
CCTTTCTACC CTGTGGATTA CACTGCAGAC CAGCACGGCA CCTGTGGGGA TGGGCTCCTG
CAGCCTGGGG AGGAGTGTGA CGACGGTAAC AGCGATGTGG GTGACGACTG CATCCGCTGT
CACCGTGCCT ACTGTGGAGA TGGTCACCGG CATGAGGGTG TGGAGGACTG TGACGGCTCT
GACTTTGGCT ACCTGACATG CGAGACCTAT CTCCCTGGGT CATATGGAGA CCTGCAATGC
ACCCAGTACT GCTACATCGA CTCCACGCCC TGCCGCTACT TCACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16232
Accession Version NM_080538.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product acetylcholinesterase collagenic tail peptide isoform II precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK128401.1, AF057036.1 and AI554171.1. On Oct 27, 2006 this sequence version replaced gi:18105017. Summary: This gene encodes the subunit of a collagen-like molecule associated with acetylcholinesterase in skeletal muscle. Each molecule is composed of three identical subunits. Each subunit contains a proline-rich attachment domain (PRAD) that binds an acetylcholinesterase tetramer to anchor the catalytic subunit of the enzyme to the basal lamina. Mutations in this gene are associated with endplate acetylcholinesterase deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (II) utilizes alternate exon 1A that is not present in variant I. The use of this exon results in a unique N-terminus compared to isoform I. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK128401.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGACGGGCT CATCATTCAG CCTCGCTCAT CTGCTCATCA TTTCAGGACT GCTCTGTTAC 
TCGGCAGGCT GCTTGGCCCT TCCCAGCCTG GATCAGAAGA AGCGTGGTGG CCACAAAGCA
TGCTGCCTGC TGACGCCTCC TCCACCACCA CTGTTCCCAC CACCATTCTT CAGAGGTGGC
CGAAGTCCGC TTCTCTCCCC AGACATGAAG AATCTCATGC TGGAACTGGA GACCTCGCAG
TCCCCGTGCA TGCAAGGCTC GCTAGGCTCC CCTGGGCCTC CCGGCCCCCA GGGTCCACCG
GGGCTTCCTG GCAAGACAGG ACCAAAGGGA GAAAAGGGGG AGCTTGGCCG ACCAGGAAGG
AAGGGTAGAC CTGGCCCCCC AGGTGTTCCT GGCATGCCTG GGCCCATCGG TTGGCCAGGC
CCTGAAGGAC CCAGGGGTGA AAAAGGTGAC CTGGGTATGA TGGGCTTGCC AGGGTCAAGA
GGACCAATGG GCTCCAAGGG CTACCCTGGA TCCAGAGGGG AAAAGGGATC CAGAGGTGAA
AAGGGTGACC TGGGTCCCAA AGGAGAAAAG GGTTTCCCAG GATTTCCTGG AATGTTGGGG
CAGAAAGGTG AAATGGGTCC AAAAGGTGAA CCTGGGATAG CAGGACACCG AGGACCCACA
GGAAGACCAG GAAAACGAGG CAAGCAGGGA CAGAAAGGGG ATAGTGGAGT TATGGGCCCA
CCAGGCAAGC CTGGGCCTTC TGGTCAACCT GGCCGTCCGG GGCCCCCAGG CCCCCCACCT
GCAGGACAAC TTATAATGGG ACCCAAAGGG GAAAGAGGAT TTCCCGGGCC TCCAGGAAGA
TGTCTTTGTG GACCCACTAT GAATGTGAAT AACCCTTCCT ACGGGGAATC TGTGTATGGG
CCCAGTTCCC CGCGAGTTCC TGTGATTTTT GTGGTCAACA ACCAGGAGGA GCTTGAGAGG
CTGAACACCC AAAACGCCAT TGCCTTCCGC AGAGACCAGA GATCTCTGTA CTTCAAGGAC
AGCCTTGGCT GGCTCCCCAT CCAGCTGACC CCTTTCTACC CTGTGGATTA CACTGCAGAC
CAGCACGGCA CCTGTGGGGA TGGGCTCCTG CAGCCTGGGG AGGAGTGTGA CGACGGTAAC
AGCGATGTGG GTGACGACTG CATCCGCTGT CACCGTGCCT ACTGTGGAGA TGGTCACCGG
CATGAGGGTG TGGAGGACTG TGACGGCTCT GACTTTGGCT ACCTGACATG CGAGACCTAT
CTCCCTGGGT CATATGGAGA CCTGCAATGC ACCCAGTACT GCTACATCGA CTCCACGCCC
TGCCGCTACT TCACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_536799.1
CDS286..1623
Misc Feature(1)181..183(+)
Misc Feature(2)406..456(+)
Misc Feature(3)766..942(+)
Misc Feature(4)1372..>1446(+)
Misc Feature(5)1378..1446(+)
Exon (1)1..361
Gene:COLQ
Gene Synonym:
Exon (2)362..474
Gene:COLQ
Gene Synonym:
Exon (3)475..576
Gene:COLQ
Gene Synonym:
Exon (4)577..621
Gene:COLQ
Gene Synonym:
Exon (5)622..648
Gene:COLQ
Gene Synonym:
Exon (6)649..720
Gene:COLQ
Gene Synonym:
Exon (7)721..783
Gene:COLQ
Gene Synonym:
Exon (8)784..810
Gene:COLQ
Gene Synonym:
Exon (9)811..855
Gene:COLQ
Gene Synonym:
Exon (10)856..891
Gene:COLQ
Gene Synonym:
Exon (11)892..972
Gene:COLQ
Gene Synonym:
Exon (12)973..1069
Gene:COLQ
Gene Synonym:
Exon (13)1070..1209
Gene:COLQ
Gene Synonym:
Exon (14)1210..1329
Gene:COLQ
Gene Synonym:
Exon (15)1330..1450
Gene:COLQ
Gene Synonym:
Exon (16)1451..1553
Gene:COLQ
Gene Synonym:
Exon (17)1554..3134
Gene:COLQ
Gene Synonym:
Translation
Position Chain Variation Link
6 6 c, g dbSNP:758810369
11 11 c, t dbSNP:753310591
51 51 g, t dbSNP:111272959
130 130 a, g dbSNP:536037695
170 170 a, g dbSNP:779571070
177 177 c, t dbSNP:567484955
185 185 g, t dbSNP:56113400
197 197 a, g dbSNP:550563779
221 221 c, t dbSNP:537915507
222 222 a, g dbSNP:182865456
224 224 c, t dbSNP:56934790
225 225 a, g dbSNP:772773496
232 232 a, c dbSNP:58156300
243 243 a, g dbSNP:771450958
256 256 c, t dbSNP:377500904
259 259 c, t dbSNP:373780924
265 265 c, g, t dbSNP:551469530
267 267 c, g dbSNP:778040042
286 286 a, g dbSNP:540751454
292 292 a, g dbSNP:756486084
297 297 -, at dbSNP:772340759
309 309 c, t dbSNP:191080619
330 330 a, c dbSNP:376962147
336 336 a, g dbSNP:372692677
345 345 c, g dbSNP:561547071
347 347 c, t dbSNP:370032148
348 348 a, g dbSNP:368094947
364 364 c, t dbSNP:753693817
372 372 a, c dbSNP:138891500
375 375 a, g dbSNP:759795366
376 376 a, g dbSNP:774553533
387 387 c, g dbSNP:532869144
388 388 c, t dbSNP:763096476
389 389 a, c, g dbSNP:761717418
390 390 c, t dbSNP:551550075
392 392 g, t dbSNP:191657758
396 396 c, t dbSNP:777090135
397 397 -, c dbSNP:761285078
397 397 c, t dbSNP:769136046
407 407 a, g dbSNP:746714301
410 410 a, g dbSNP:564244822
411 411 c, t dbSNP:758060872
414 414 a, g dbSNP:745419074
417 417 a, g dbSNP:778242187
419 419 a, c, t dbSNP:774271810
420 420 a, g dbSNP:777613214
421 421 c, t dbSNP:755892125
422 422 c, t dbSNP:768517440
426 426 -, tcc dbSNP:753308023
428 428 c, t dbSNP:766617611
430 430 c, t dbSNP:763036328
435 435 a, g dbSNP:750564606
436 436 c, t dbSNP:765195642
442 442 a, c dbSNP:762446165
446 446 c, t dbSNP:188737921
449 449 c, t dbSNP:777261321
460 460 a, g dbSNP:769226066
464 464 g, t dbSNP:761127569
467 467 a, g dbSNP:775959815
469 469 a, g dbSNP:771877645
473 473 c, t dbSNP:374700344
488 488 a, t dbSNP:762838086
490 490 a, g dbSNP:770561043
496 496 a, g dbSNP:748826424
497 497 a, g dbSNP:777215134
501 501 c, t dbSNP:372432709
504 504 a, g dbSNP:748046000
505 505 c, t dbSNP:369308750
512 512 c, t dbSNP:754712383
518 518 c, t dbSNP:746757636
522 522 a, g dbSNP:144897863
530 530 c, t dbSNP:757464366
531 531 a, g dbSNP:753997328
537 537 a, g dbSNP:764250328
542 542 g, t dbSNP:369852257
543 543 c, t dbSNP:756730529
545 545 c, t dbSNP:531584926
546 546 a, g, t dbSNP:115201284
555 555 c, t dbSNP:2305611
568 568 a, g dbSNP:201507876
584 584 c, t dbSNP:776165035
592 592 a, c dbSNP:768502040
596 596 g, t dbSNP:746943756
611 611 a, t dbSNP:775189965
612 612 a, g dbSNP:771988325
623 623 c, g dbSNP:781191924
632 632 -, g dbSNP:759874172
634 634 c, g, t dbSNP:143766249
635 635 a, g dbSNP:201856842
643 643 a, g dbSNP:758861218
644 644 a, g dbSNP:750806839
646 646 a, g dbSNP:142980906
653 653 g, t dbSNP:755030467
655 655 c, t dbSNP:751526069
657 657 c, t dbSNP:766356664
660 660 c, t dbSNP:762810817
663 663 c, g dbSNP:772842453
678 678 a, c dbSNP:764397858
681 681 g, t dbSNP:575929335
683 683 a, c dbSNP:373840796
684 684 c, t dbSNP:775589975
693 693 c, t dbSNP:757867510
694 694 a, g dbSNP:370976269
695 695 a, g dbSNP:774853684
705 705 c, t dbSNP:771279997
706 706 a, c dbSNP:114544830
714 714 a, c dbSNP:777953780
715 715 c, t dbSNP:755848079
729 729 -, a dbSNP:770006712
729 729 a, g dbSNP:770038933
731 731 c, g dbSNP:748461738
733 733 a, g dbSNP:780992593
736 736 a, c dbSNP:768382539
739 739 g, t dbSNP:376385207
747 747 a, g dbSNP:779407765
761 761 c, g dbSNP:104893734
772 772 a, g dbSNP:750324839
777 777 c, t dbSNP:113843907
778 778 c, t dbSNP:757056994
788 788 a, c dbSNP:751714432
794 794 g, t dbSNP:766563349
808 808 a, g dbSNP:144705312
810 810 a, g dbSNP:750472473
815 815 c, g dbSNP:201850242
816 816 c, t dbSNP:149370622
818 818 c, g dbSNP:757905303
824 824 a, g dbSNP:377364923
839 839 a, g dbSNP:764640385
853 853 a, g dbSNP:760952573
855 855 a, g dbSNP:775885011
856 856 a, g dbSNP:769665618
861 861 c, t dbSNP:267599659
865 865 a, g dbSNP:374783562
869 869 a, t dbSNP:776357105
876 876 a, c dbSNP:138830715
877 877 a, g dbSNP:746744618
895 895 g, t dbSNP:104893733
898 898 a, g dbSNP:780605254
906 906 a, g dbSNP:777785311
907 907 a, g dbSNP:769908780
909 909 a, g dbSNP:112673051
924 924 a, c dbSNP:781736167
925 925 g, t dbSNP:755424266
929 929 a, c, g dbSNP:780362486
934 934 c, g, t dbSNP:770045897
935 935 a, g dbSNP:764737302
942 942 c, t dbSNP:756797522
949 949 a, g dbSNP:753247779
961 961 c, g, t dbSNP:760669036
962 962 a, g dbSNP:200770910
965 965 a, g dbSNP:767419139
966 966 a, c dbSNP:763329693
968 968 a, g dbSNP:79655731
973 973 g, t dbSNP:104893735
978 978 a, g dbSNP:755703073
986 986 a, g dbSNP:752192353
992 992 c, g dbSNP:146687198
996 996 c, t dbSNP:754845086
1004 1004 c, t dbSNP:200479277
1014 1014 a, g dbSNP:373597557
1022 1022 c, t dbSNP:762151906
1024 1024 g, t dbSNP:754017581
1038 1038 c, g dbSNP:745798750
1040 1040 a, g dbSNP:201158622
1041 1041 c, t dbSNP:760774340
1043 1043 -, c dbSNP:759911990
1043 1043 c, t dbSNP:146619514
1044 1044 a, g dbSNP:150061580
1053 1053 a, t dbSNP:759931610
1055 1055 g, t dbSNP:774848044
1057 1057 a, c dbSNP:771189959
1063 1063 c, t dbSNP:749610650
1066 1066 g, t dbSNP:777577778
1067 1067 c, t dbSNP:373735085
1075 1075 c, t dbSNP:763262836
1078 1078 a, g dbSNP:773686063
1090 1090 a, c, g dbSNP:114896510
1094 1094 c, g dbSNP:778156526
1099 1099 a, t dbSNP:121908922
1108 1108 c, g dbSNP:746460292
1110 1110 a, c, t dbSNP:758307097
1111 1111 a, c, g dbSNP:200403785
1113 1113 g, t dbSNP:756396914
1124 1124 a, g dbSNP:752905204
1126 1126 g, t dbSNP:374490483
1144 1144 a, g dbSNP:755020019
1159 1159 c, g dbSNP:751621776
1164 1164 c, t dbSNP:766821521
1167 1167 -, c dbSNP:763209840
1169 1169 g, t dbSNP:763518486
1173 1173 a, g, t dbSNP:765680430
1177 1177 a, g dbSNP:184245561
1180 1180 -, t dbSNP:773636951
1181 1181 a, g dbSNP:200232229
1183 1183 a, g dbSNP:776425306
1189 1189 a, c, g dbSNP:6782980
1192 1192 a, t dbSNP:143918191
1193 1193 c, g dbSNP:550979119
1196 1196 c, t dbSNP:370201862
1197 1197 a, g dbSNP:771944316
1198 1198 c, t dbSNP:121908924
1199 1199 a, g dbSNP:778740405
1222 1222 a, t dbSNP:369551954
1224 1224 a, c dbSNP:760343679
1225 1225 a, c dbSNP:201036462
1233 1233 a, g dbSNP:113488563
1237 1237 a, c dbSNP:771559323
1242 1242 c, g dbSNP:758991725
1254 1254 a, c dbSNP:774279832
1255 1255 c, g dbSNP:113708721
1260 1260 c, t dbSNP:770819352
1272 1272 a, c dbSNP:749107627
1273 1273 c, t dbSNP:138818971
1274 1274 a, g dbSNP:375225755
1279 1279 a, g dbSNP:184944796
1281 1281 a, c, g dbSNP:758554049
1286 1286 a, g dbSNP:149532541
1291 1291 a, c dbSNP:750489224
1292 1292 c, t dbSNP:779583406
1294 1294 c, t dbSNP:757815714
1313 1313 c, g dbSNP:754194997
1317 1317 g, t dbSNP:764516134
1322 1322 c, g dbSNP:760303798
1324 1324 a, g dbSNP:752410020
1327 1327 c, g dbSNP:372064982
1334 1334 c, t dbSNP:779104097
1336 1336 c, t dbSNP:116828761
1337 1337 -, c dbSNP:769982050
1340 1340 c, t dbSNP:754357969
1341 1341 c, g dbSNP:778085094
1342 1342 c, t dbSNP:756508281
1344 1344 c, g dbSNP:752967536
1363 1363 a, g, t dbSNP:116373583
1371 1371 c, t dbSNP:759169923
1372 1372 a, g dbSNP:202019416
1383 1383 g, t dbSNP:751113924
1385 1385 a, g dbSNP:765874335
1402 1402 a, g dbSNP:762306354
1408 1408 c, g dbSNP:140955551
1410 1410 a, c, g dbSNP:116503231
1412 1412 c, g dbSNP:368575417
1416 1416 c, t dbSNP:539100854
1419 1419 c, t dbSNP:149852377
1420 1420 a, g dbSNP:774638847
1428 1428 c, t dbSNP:771047223
1429 1429 a, g dbSNP:749266800
1440 1440 c, t dbSNP:548322139
1441 1441 a, c, g dbSNP:748582765
1449 1449 c, t dbSNP:199930637
1450 1450 c, t dbSNP:779594053
1451 1451 a, g dbSNP:375272767
1460 1460 a, g dbSNP:140670616
1464 1464 c, t dbSNP:753830857
1466 1466 a, g dbSNP:764064240
1467 1467 -, c dbSNP:771866680
1467 1467 c, t dbSNP:760567044
1475 1475 a, g dbSNP:752498913
1483 1483 a, c, t dbSNP:139574075
1488 1488 a, t dbSNP:773394999
1492 1492 a, g dbSNP:769772442
1503 1503 c, t dbSNP:55866379
1509 1509 c, t dbSNP:777147864
1510 1510 a, g dbSNP:769114264
1529 1529 a, t dbSNP:753690228
1532 1532 a, c, t dbSNP:536042715
1533 1533 a, g, t dbSNP:745502271
1536 1536 c, t dbSNP:185829251
1537 1537 a, g dbSNP:141721093
1541 1541 a, c dbSNP:550616958
1543 1543 c, t dbSNP:753348773
1544 1544 a, c dbSNP:121908923
1550 1550 c, t dbSNP:756084974
1554 1554 g, t dbSNP:754755194
1572 1572 a, g dbSNP:369122841
1576 1576 a, g, t dbSNP:375215281
1578 1578 c, g dbSNP:753978763
1589 1589 a, g dbSNP:764155601
1591 1591 a, c dbSNP:371867135
1593 1593 a, c, g, t dbSNP:73818504
1594 1594 a, g, t dbSNP:770753693
1599 1599 c, g dbSNP:748932004
1600 1600 a, c dbSNP:772919447
1601 1601 -, c dbSNP:757053860
1602 1602 a, g dbSNP:769428838
1605 1605 c, t dbSNP:747648795
1609 1609 c, t dbSNP:368932156
1610 1610 a, g dbSNP:754915644
1614 1614 c, t dbSNP:746832080
1621 1621 c, t dbSNP:188841362
1627 1627 -, t dbSNP:748930865
1627 1627 a, c, t dbSNP:754063500
1628 1628 c, t dbSNP:753583108
1629 1629 a, g, t dbSNP:144621591
1640 1640 a, g dbSNP:142437859
1642 1642 a, g dbSNP:760065319
1643 1643 a, g dbSNP:766337085
1647 1647 a, g dbSNP:774910654
1648 1648 c, t dbSNP:766842695
1662 1662 a, g dbSNP:763339889
1666 1666 g, t dbSNP:773025427
1667 1667 c, t dbSNP:769535072
1682 1682 c, t dbSNP:747654042
1683 1683 a, g dbSNP:776185686
1708 1708 a, g dbSNP:768534998
1713 1713 c, t dbSNP:746964365
1718 1718 a, t dbSNP:779900149
1722 1722 c, t dbSNP:758203590
1726 1726 a, g dbSNP:745613708
1728 1728 a, g dbSNP:761993988
1737 1737 a, g dbSNP:778002170
1747 1747 a, t dbSNP:184812820
1757 1757 a, g dbSNP:752767490
1767 1767 c, g dbSNP:375831415
1771 1771 -, actca dbSNP:777457693
1777 1777 g, t dbSNP:755501265
1779 1779 c, t dbSNP:752112752
1792 1792 a, t dbSNP:550256310
1812 1812 c, t dbSNP:749985637
1833 1833 -, aa dbSNP:138019222
1835 1835 c, t dbSNP:530125773
1858 1858 c, t dbSNP:537145282
1869 1869 c, t dbSNP:374251957
1870 1870 a, g dbSNP:551512511
1899 1899 a, g dbSNP:766923758
1910 1910 c, t dbSNP:769285503
1921 1921 c, t dbSNP:144067075
1936 1936 a, g dbSNP:565997789
1942 1942 c, t dbSNP:549636072
1967 1967 c, t dbSNP:2278961
1988 1988 a, t dbSNP:2278962
2019 2019 a, c dbSNP:372672661
2030 2030 c, t dbSNP:569980572
2031 2031 a, g dbSNP:771628214
2036 2036 -, t dbSNP:534228650
2044 2044 a, g dbSNP:533015053
2045 2045 a, g dbSNP:564176947
2049 2049 a, g dbSNP:763440701
2087 2087 a, g, t dbSNP:770219393
2114 2114 a, g dbSNP:541202497
2115 2115 a, g dbSNP:116231717
2127 2127 a, g dbSNP:746360529
2138 2138 c, t dbSNP:10154896
2139 2139 a, g dbSNP:544927968
2173 2173 c, t dbSNP:576307598
2177 2177 c, t dbSNP:557314953
2178 2178 g, t dbSNP:116450586
2206 2206 a, g dbSNP:747056584
2218 2218 -, g dbSNP:149076823
2326 2326 c, g dbSNP:537057072
2327 2327 a, g dbSNP:577687841
2374 2374 c, t dbSNP:368981773
2429 2429 c, t dbSNP:534825077
2471 2471 c, t dbSNP:550045499
2527 2527 c, t dbSNP:181965246
2549 2549 c, g dbSNP:536136335
2568 2568 a, g dbSNP:372867726
2600 2600 c, t dbSNP:549747763
2617 2617 a, g dbSNP:77521642
2621 2621 c, t dbSNP:570530635
2624 2624 a, g dbSNP:3846128
2656 2656 c, t dbSNP:771512608
2691 2691 a, g dbSNP:527411340
2696 2696 a, g dbSNP:561918872
2700 2700 a, g dbSNP:541699261
2706 2706 c, t dbSNP:749123750
2707 2707 a, g dbSNP:376202299
2801 2801 c, t dbSNP:747653111
2871 2871 c, t dbSNP:373209743
2894 2894 a, g dbSNP:779804779
2900 2900 c, t dbSNP:572408115
2903 2903 c, t dbSNP:146270961
2954 2954 c, g dbSNP:756102599
2956 2956 c, t dbSNP:778433921
2957 2957 a, g dbSNP:543377033
2970 2970 c, t dbSNP:189542753
2981 2981 c, t dbSNP:757814018
2989 2989 a, g dbSNP:376129145
3009 3009 a, g dbSNP:376193770
3018 3018 c, t dbSNP:3274
3021 3021 c, g dbSNP:756739237
3030 3030 a, g dbSNP:113737086
3113 3113 a, c dbSNP:751038602

Target ORF information:

RefSeq Version NM_080538
Organism Homo sapiens (human)
Definition Homo sapiens collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant II, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_080538

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGACGGGCT CATCATTCAG CCTCGCTCAT CTGCTCATCA TTTCAGGACT GCTCTGTTAC 
TCGGCAGGCT GCTTGGCCCT TCCCAGCCTG GATCAGAAGA AGCGTGGTGG CCACAAAGCA
TGCTGCCTGC TGACGCCTCC TCCACCACCA CTGTTCCCAC CACCATTCTT CAGAGGTGGC
CGAAGTCCGC TTCTCTCCCC AGACATGAAG AATCTCATGC TGGAACTGGA GACCTCGCAG
TCCCCGTGCA TGCAAGGCTC GCTAGGCTCC CCTGGGCCTC CCGGCCCCCA GGGTCCACCG
GGGCTTCCTG GCAAGACAGG ACCAAAGGGA GAAAAGGGGG AGCTTGGCCG ACCAGGAAGG
AAGGGTAGAC CTGGCCCCCC AGGTGTTCCT GGCATGCCTG GGCCCATCGG TTGGCCAGGC
CCTGAAGGAC CCAGGGGTGA AAAAGGTGAC CTGGGTATGA TGGGCTTGCC AGGGTCAAGA
GGACCAATGG GCTCCAAGGG CTACCCTGGA TCCAGAGGGG AAAAGGGATC CAGAGGTGAA
AAGGGTGACC TGGGTCCCAA AGGAGAAAAG GGTTTCCCAG GATTTCCTGG AATGTTGGGG
CAGAAAGGTG AAATGGGTCC AAAAGGTGAA CCTGGGATAG CAGGACACCG AGGACCCACA
GGAAGACCAG GAAAACGAGG CAAGCAGGGA CAGAAAGGGG ATAGTGGAGT TATGGGCCCA
CCAGGCAAGC CTGGGCCTTC TGGTCAACCT GGCCGTCCGG GGCCCCCAGG CCCCCCACCT
GCAGGACAAC TTATAATGGG ACCCAAAGGG GAAAGAGGAT TTCCCGGGCC TCCAGGAAGA
TGTCTTTGTG GACCCACTAT GAATGTGAAT AACCCTTCCT ACGGGGAATC TGTGTATGGG
CCCAGTTCCC CGCGAGTTCC TGTGATTTTT GTGGTCAACA ACCAGGAGGA GCTTGAGAGG
CTGAACACCC AAAACGCCAT TGCCTTCCGC AGAGACCAGA GATCTCTGTA CTTCAAGGAC
AGCCTTGGCT GGCTCCCCAT CCAGCTGACC CCTTTCTACC CTGTGGATTA CACTGCAGAC
CAGCACGGCA CCTGTGGGGA TGGGCTCCTG CAGCCTGGGG AGGAGTGTGA CGACGGTAAC
AGCGATGTGG GTGACGACTG CATCCGCTGT CACCGTGCCT ACTGTGGAGA TGGTCACCGG
CATGAGGGTG TGGAGGACTG TGACGGCTCT GACTTTGGCT ACCTGACATG CGAGACCTAT
CTCCCTGGGT CATATGGAGA CCTGCAATGC ACCCAGTACT GCTACATCGA CTCCACGCCC
TGCCGCTACT TCACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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