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HIST1H4I cDNA ORF clone, Homo sapiens (human)

Gene Symbol HIST1H4I
Entrez Gene ID 8294
Full Name histone cluster 1, H4i
Synonyms H4/m, H4FM, H4M
General protein information
Preferred Names
histone H4
Names
histone H4
histone 1, H4i
histone family member
Histone 4 family, member M
H4 histone family, member M
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.33

Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015]. lac of sum
Disorder MIM:

602833

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_003495 NP_003486 histone H4

KEGG
hsa05322 Systemic lupus erythematosus
hsa05203 Viral carcinogenesis
hsa05034 Alcoholism
Reactome
R-HSA-1643685 Disease
R-HSA-977225 Amyloid formation
R-HSA-162582 Signal Transduction
R-HSA-194315 Signaling by Rho GTPases
R-HSA-195258 RHO GTPase Effectors
R-HSA-5625740 RHO GTPases activate PKNs
R-HSA-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
R-HSA-195721 Signaling by Wnt
R-HSA-74160 Gene Expression
R-HSA-73728 RNA Polymerase I Promoter Opening
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-504046 RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
R-HSA-73854 RNA Polymerase I Promoter Clearance
R-HSA-73864 RNA Polymerase I Transcription
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-1640170 Cell Cycle
R-HSA-211000 Gene Silencing by RNA
R-HSA-212165 Epigenetic regulation of gene expression
R-HSA-5250941 Negative epigenetic regulation of rRNA expression
R-HSA-427359 SIRT1 negatively regulates rRNA Expression
R-HSA-5334118 DNA methylation
R-HSA-69278 Cell Cycle, Mitotic
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-68875 Mitotic Prophase
R-HSA-68886 M Phase
R-HSA-2299718 Condensation of Prophase Chromosomes
R-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-HSA-212300 PRC2 methylates histones and DNA
R-HSA-69481 G2/M Checkpoints
R-HSA-69473 G2/M DNA damage checkpoint
R-HSA-1500620 Meiosis
R-HSA-1221632 Meiotic synapsis
R-HSA-157579 Telomere Maintenance
R-HSA-912446 Meiotic recombination
R-HSA-171306 Packaging Of Telomere Ends
R-HSA-73886 Chromosome Maintenance
R-HSA-774815 Nucleosome assembly
R-HSA-2262752 Cellular responses to stress
R-HSA-4839726 Chromatin organization
R-HSA-3247509 Chromatin modifying enzymes
R-HSA-3214847 HATs acetylate histones
R-HSA-2559580 Oxidative Stress Induced Senescence
R-HSA-2559586 DNA Damage/Telomere Stress Induced Senescence
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-2559583 Cellular Senescence
R-HSA-3214842 HDMs demethylate histones
R-HSA-3214841 PKMTs methylate histone lysines
R-HSA-3214815 HDACs deacetylate histones
R-HSA-3214858 RMTs methylate histone arginines
Pathway Interaction Database
hdac_classiii_pathway Signaling events mediated by HDAC Class III
WikiPathways
WP619 Type II interferon signaling (IFNG)
WP2361 Gastric cancer network 1


Homo sapiens (human) HIST1H4I NP_003486.1
Pan troglodytes (chimpanzee) LOC748545 XP_001172771.2
Macaca mulatta (Rhesus monkey) LOC695323 XP_001083839.1
Bos taurus (cattle) LOC527645 XP_002697516.1
Mus musculus (house mouse) Hist1h4h NP_694813.1
Rattus norvegicus (Norway rat) Hist1h4a XP_006254042.1
Gallus gallus (chicken) HIST1H46L4 XP_003640418.1
Danio rerio (zebrafish) LOC794549 XP_005172451.1
Drosophila melanogaster (fruit fly) His4:CG33885 NP_001027342.1
Caenorhabditis elegans his-14 NP_496889.1
Arabidopsis thaliana (thale cress) HIS4 NP_180441.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC101731642 XP_004919466.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HIST1H4I gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIST1H4I cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu30070
NM_003495 Homo sapiens histone cluster 1, H4i (HIST1H4I), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu30070
Accession Version NM_003495.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 312bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 18-AUG-2015
Organism Homo sapiens (human)
Product histone H4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB000905.1. On Jan 10, 2002 this sequence version replaced gi:4504320. Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGTCAGGAC GCGGCAAAGG AGGTAAGGGC CTGGGGAAAG GGGGTGCCAA GCGCCACCGC 
AAGGTGCTGC GCGACAACAT CCAGGGTATC ACCAAGCCAG CCATTCGGCG CCTTGCTCGC
CGCGGCGGCG TGAAGCGCAT TTCTGGCCTC ATCTATGAGG AGACCCGCGG AGTGTTGAAG
GTGTTCCTGG AGAACGTGAT CCGGGACGCC GTGACCTACA CGGAGCACGC CAAGCGCAAG
ACGGTCACCG CCATGGACGT GGTCTACGCG CTCAAGCGCC AGGGCCGCAC CCTCTATGGC
TTCGGCGGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_003486.1
CDS1..312
Misc Feature(1)1..309(+)
Misc Feature(2)4..6(+)
Misc Feature(3)4..6(+)
Misc Feature(4)10..12(+)
Misc Feature(5)10..12(+)
Misc Feature(6)16..18(+)
Misc Feature(7)16..18(+)
Misc Feature(8)25..27(+)
Misc Feature(9)25..27(+)
Misc Feature(10)37..39(+)
Misc Feature(11)37..39(+)
Misc Feature(12)49..51(+)
Misc Feature(13)49..51(+)
Misc Feature(14)52..303(+)
Misc Feature(15)61..63(+)
Misc Feature(16)94..96(+)
Misc Feature(17)100..273(+)
Misc Feature(18)121..135(+)
Misc Feature(19)133..291(+)
Misc Feature(20)142..144(+)
Misc Feature(21)154..156(+)
Misc Feature(22)205..279(+)
Misc Feature(23)265..267(+)
Misc Feature(24)274..276(+)
Misc Feature(25)274..276(+)
Misc Feature(26)289..303(+)
Exon (1)1..370
Gene:HIST1H4I
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, g dbSNP:763652001
9 9 -, cg dbSNP:779965916
10 10 c, g dbSNP:751062963
11 11 a, g dbSNP:757221402
14 14 a, g dbSNP:767301868
17 17 a, g dbSNP:750256756
18 18 a, g dbSNP:755827089
19 19 a, g dbSNP:779964017
21 21 a, g dbSNP:749574795
23 23 c, g dbSNP:755241311
27 27 a, g dbSNP:369247236
29 29 c, g dbSNP:140775845
31 31 c, t dbSNP:772653571
39 39 a, g dbSNP:564129943
40 40 a, g dbSNP:373924281
41 41 a, g dbSNP:747515954
42 42 a, g dbSNP:771249191
43 43 g, t dbSNP:560111648
47 47 c, t dbSNP:762658565
48 48 c, t dbSNP:763668364
53 53 -, c dbSNP:753877604
55 55 c, t dbSNP:773865845
56 56 -, c dbSNP:754932977
59 59 g, t dbSNP:761180213
60 60 c, t dbSNP:767522132
64 64 g, t dbSNP:143050720
66 66 a, g dbSNP:575103151
67 67 c, t dbSNP:760643847
70 70 c, t dbSNP:766338156
77 77 a, g dbSNP:753685066
78 78 c, t dbSNP:377361526
80 80 c, t dbSNP:779226325
85 85 g, t dbSNP:752849854
86 86 -, t dbSNP:778931172
86 86 a, g dbSNP:758444195
90 90 -, c dbSNP:748268091
92 92 c, t dbSNP:777882267
96 96 a, g dbSNP:747527283
99 99 a, g dbSNP:151220380
102 102 c, t dbSNP:781539533
105 105 c, t dbSNP:746171478
106 106 -, ggcgcctt dbSNP:771984935
109 109 c, t dbSNP:768322550
113 113 -, a dbSNP:777989743
118 118 c, t dbSNP:773953710
121 121 c, t dbSNP:761496441
138 138 c, t dbSNP:771531425
140 140 c, t dbSNP:772770011
147 147 c, t dbSNP:528761233
148 148 c, t dbSNP:766356271
153 153 c, g dbSNP:753701748
155 155 a, g, t dbSNP:759362207
162 162 a, g dbSNP:769017931
165 165 c, g, t dbSNP:752906607
166 166 c, t dbSNP:200159567
170 170 a, g dbSNP:140361782
171 171 a, g dbSNP:565433112
181 181 a, g dbSNP:781629621
182 182 c, t dbSNP:746265355
184 184 a, t dbSNP:770184078
187 187 c, t dbSNP:780445663
192 192 -, a dbSNP:747189597
192 192 a, g dbSNP:150377743
197 197 g, t dbSNP:368987850
208 208 c, g dbSNP:772605269
210 210 c, t dbSNP:760177834
213 213 c, g dbSNP:771021223
214 214 -, c dbSNP:771322297
225 225 a, g dbSNP:776747593
226 226 c, t dbSNP:759519873
228 228 a, c, t dbSNP:531123491
229 229 a, g dbSNP:373877069
236 236 a, c, g dbSNP:116193614
242 242 c, t dbSNP:776948616
243 243 a, g dbSNP:764317875
249 249 c, g, t dbSNP:751611984
250 250 a, g dbSNP:370763512
252 252 c, t dbSNP:750977807
254 254 c, t dbSNP:756601298
255 255 a, g dbSNP:780353227
259 259 a, g dbSNP:376974407
264 264 c, g dbSNP:567727351
267 267 c, t dbSNP:777436970
268 268 a, g dbSNP:746626210
273 273 c, g, t dbSNP:570548268
277 277 c, t dbSNP:745864614
285 285 a, c, g, t dbSNP:370966198
286 286 c, t dbSNP:373660840
297 297 c, t dbSNP:775159553
300 300 c, t dbSNP:762759026
303 303 c, t dbSNP:764255292
306 306 c, t dbSNP:61742445
312 312 a, c dbSNP:762000798
313 313 a, g dbSNP:767643496
315 315 a, g dbSNP:112207956
316 316 a, g dbSNP:750500808
323 323 a, g dbSNP:756622079
329 329 -, cagt dbSNP:776957502
329 329 c, t dbSNP:766853791
330 330 a, g dbSNP:754179018
337 337 a, c, t dbSNP:546553580
340 340 c, t dbSNP:779121479
341 341 a, t dbSNP:746712235
345 345 a, t dbSNP:202114057
349 349 c, t dbSNP:756961772
350 350 c, g dbSNP:780853367
353 353 c, t dbSNP:745317020
359 359 -, ggt dbSNP:201244252
362 362 c, t dbSNP:769639236
363 363 c, t dbSNP:775457642
367 367 a, g dbSNP:143475637
368 368 c, g dbSNP:188731680

Target ORF information:

RefSeq Version NM_003495
Organism Homo sapiens (human)
Definition Homo sapiens histone cluster 1, H4i (HIST1H4I), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003495

ORF Insert Sequence:

1
61
121
181
241
301
ATGTCAGGAC GCGGCAAAGG AGGTAAGGGC CTGGGGAAAG GGGGTGCCAA GCGCCACCGC 
AAGGTGCTGC GCGACAACAT CCAGGGTATC ACCAAGCCAG CCATTCGGCG CCTTGCTCGC
CGCGGCGGCG TGAAGCGCAT TTCTGGCCTC ATCTATGAGG AGACCCGCGG AGTGTTGAAG
GTGTTCCTGG AGAACGTGAT CCGGGACGCC GTGACCTACA CGGAGCACGC CAAGCGCAAG
ACGGTCACCG CCATGGACGT GGTCTACGCG CTCAAGCGCC AGGGCCGCAC CCTCTATGGC
TTCGGCGGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Human tNASP promotes in vitro nucleosome assembly with histone H3.3
Biochemistry 54 (5), 1171-1179 (2015)
Kato D, Osakabe A, Tachiwana H, Tanaka H and Kurumizaka H.


book

A proteome-scale map of the human interactome network
Cell 159 (5), 1212-1226 (2014)
Rolland T, Tasan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabasi AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP and Vidal M.


book

Structural insights into recognition of acetylated histone ligands by the BRPF1 bromodomain
FEBS Lett. 588 (21), 3844-3854 (2014)
Lubula MY, Eckenroth BE, Carlson S, Poplawski A, Chruszcz M and Glass KC.


book

Molecular functions of the TLE tetramerization domain in Wnt target gene repression
EMBO J. 33 (7), 719-731 (2014)
Chodaparambil JV, Pate KT, Hepler MR, Tsai BP, Muthurajan UM, Luger K, Waterman ML and Weis WI.


book

Structure of human nucleosome containing the testis-specific histone variant TSH2B
Acta Crystallogr F Struct Biol Commun 70 (PT 4), 444-449 (2014)
Urahama T, Horikoshi N, Osakabe A, Tachiwana H and Kurumizaka H.


book

The human and mouse replication-dependent histone genes
Genomics 80 (5), 487-498 (2002)
Marzluff WF, Gongidi P, Woods KR, Jin J and Maltais LJ.


book

Isolation and characterization of two human H1 histone genes within clusters of core histone genes
Genomics 10 (4), 940-948 (1991)
Albig W, Kardalinou E, Drabent B, Zimmer A and Doenecke D.


book

Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabeling with site-specific antibodies
FEBS Lett. 253 (1-2), 141-145 (1989)
Turner BM, O'Neill LP and Allan IM.


book

A highly basic histone H4 domain bound to the sharply bent region of nucleosomal DNA
Nature 331 (6154), 365-367 (1988)
Ebralidse KK, Grachev SA and Mirzabekov AD.


book

Protein-DNA interactions in vivo upstream of a cell cycle-regulated human H4 histone gene
Science 236 (4806), 1308-1311 (1987)
Pauli,U., Chrysogelos,S., Stein,G., Stein,J. and Nick,H.


 
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