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HIST1H2BE cDNA ORF clone, Homo sapiens (human)

Gene Symbol HIST1H2BE
Entrez Gene ID 8344
Full Name histone cluster 1, H2be
Synonyms H2B.h, H2B/h, H2BFH, dJ221C16.8
General protein information
Preferred Names
histone H2B type 1-C/E/F/G/I
Names
histone H2B type 1-C/E/F/G/I
histone H2B.h
histone 1, H2be
histone H2B.1 A
H2B histone family, member H
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p22.2

Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]. lac of sum
Disorder MIM:

602805


mRNA and Protein(s)

mRNA Protein Name
NM_003523 NP_003514 histone H2B type 1-C/E/F/G/I



Homo sapiens (human) HIST1H2BE NP_003514.2
Pan troglodytes (chimpanzee) LOC471883 XP_527261.1
Canis lupus familiaris (dog) LOC100856116 XP_003640206.1
Bos taurus (cattle) HIST1H2BL XP_002686101.1
Mus musculus (house mouse) Hist1h2bl NP_835506.1

The following HIST1H2BE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIST1H2BE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu30268
NM_003523 Homo sapiens histone cluster 1, H2be (HIST1H2BE), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu30268
Clone ID Related Accession (Same CDS sequence) NM_003523
Accession Version NM_003523.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 381bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 18-AUG-2015
Organism Homo sapiens (human)
Product histone H2B type 1-C/E/F/G/I
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z80780.1. On Jun 12, 2002 this sequence version replaced gi:4504266. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BP362977.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 12860195 replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGCCTGAGC CAGCGAAATC CGCTCCCGCC CCGAAGAAGG GCTCCAAGAA GGCCGTGACC 
AAGGCGCAGA AGAAGGACGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTACTCCGTA
TACGTGTACA AGGTGCTGAA ACAGGTCCAC CCCGACACCG GCATCTCCTC TAAAGCCATG
GGGATCATGA ATTCCTTTGT CAACGACATC TTCGAGCGCA TCGCCGGCGA GGCTTCCCGC
CTGGCGCATT ACAACAAGCG CTCGACCATC ACCTCCAGGG AGATCCAGAC GGCCGTGCGC
CTGCTGCTTC CCGGGGAGCT GGCCAAGCAC GCTGTGTCAG AGGGCACCAA GGCCGTTACC
AAGTACACCA GCTCCAAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003514.2
CDS1..381
Misc Feature(1)4..6(+)
Misc Feature(2)16..18(+)
Misc Feature(3)16..18(+)
Misc Feature(4)34..36(+)
Misc Feature(5)34..36(+)
Misc Feature(6)37..39(+)
Misc Feature(7)37..39(+)
Misc Feature(8)43..45(+)
Misc Feature(9)46..48(+)
Misc Feature(10)46..48(+)
Misc Feature(11)49..51(+)
Misc Feature(12)49..51(+)
Misc Feature(13)61..63(+)
Misc Feature(14)61..63(+)
Misc Feature(15)70..72(+)
Misc Feature(16)70..72(+)
Misc Feature(17)82..372(+)
Misc Feature(18)103..105(+)
Misc Feature(19)109..111(+)
Misc Feature(20)139..141(+)
Misc Feature(21)172..174(+)
Misc Feature(22)238..240(+)
Misc Feature(23)256..258(+)
Misc Feature(24)256..258(+)
Misc Feature(25)259..261(+)
Misc Feature(26)277..279(+)
Misc Feature(27)325..327(+)
Misc Feature(28)346..348(+)
Misc Feature(29)349..351(+)
Exon (1)1..435
Gene:HIST1H2BE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003523
Organism Homo sapiens (human)
Definition Homo sapiens histone cluster 1, H2be (HIST1H2BE), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003523

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGCCTGAGC CAGCGAAATC CGCTCCCGCC CCGAAGAAGG GCTCCAAGAA GGCCGTGACC 
AAGGCGCAGA AGAAGGACGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTACTCCGTA
TACGTGTACA AGGTGCTGAA ACAGGTCCAC CCCGACACCG GCATCTCCTC TAAAGCCATG
GGGATCATGA ATTCCTTTGT CAACGACATC TTCGAGCGCA TCGCCGGCGA GGCTTCCCGC
CTGGCGCATT ACAACAAGCG CTCGACCATC ACCTCCAGGG AGATCCAGAC GGCCGTGCGC
CTGCTGCTTC CCGGGGAGCT GGCCAAGCAC GCTGTGTCAG AGGGCACCAA GGCCGTTACC
AAGTACACCA GCTCCAAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A proteome-scale map of the human interactome network
Cell 159 (5), 1212-1226 (2014)
Rolland T, Tasan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabasi AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP and Vidal M.


book

Proteomic analysis of podocyte exosome-enriched fraction from normal human urine
J Proteomics 82, 193-229 (2013)
Prunotto M, Farina A, Lane L, Pernin A, Schifferli J, Hochstrasser DF, Lescuyer P and Moll S.


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Proteomic characterization of the human sperm nucleus
Proteomics 11 (13), 2714-2726 (2011)
de Mateo S, Castillo J, Estanyol JM, Ballesca JL and Oliva R.


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MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis
Immunol. Cell Biol. 88 (8), 851-856 (2010)
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M and Stoorvogel W.


book

Substrate and functional diversity of lysine acetylation revealed by a proteomics survey
Mol. Cell 23 (4), 607-618 (2006)
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ and Zhao Y.


book

The human and mouse replication-dependent histone genes
Genomics 80 (5), 487-498 (2002)
Marzluff WF, Gongidi P, Woods KR, Jin J and Maltais LJ.


book

The human histone gene cluster at the D6S105 locus
Hum. Genet. 101 (3), 284-294 (1997)
Albig W and Doenecke D.


book

Human histone gene organization: nonregular arrangement within a large cluster
Genomics 40 (2), 314-322 (1997)
Albig W, Kioschis P, Poustka A, Meergans K and Doenecke D.


book

A novel divergently transcribed human histone H2A/H2B gene pair
DNA Seq. 1 (6), 409-413 (1991)
Dobner T, Wolf I, Mai B and Lipp M.


book

Human spleen histone H2B. Isolation and amino acid sequence
J. Biochem. 85 (2), 615-624 (1979)
Ohe,Y., Hayashi,H. and Iwai,K.


 
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