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HIST1H2BI cDNA ORF clone, Homo sapiens (human)

Gene Symbol HIST1H2BI
Entrez Gene ID 8346
Full Name histone cluster 1, H2bi
Synonyms H2B/k, H2BFK
General protein information
Preferred Names
histone H2B type 1-C/E/F/G/I
Names
histone H2B type 1-C/E/F/G/I
histone H2B.k
histone 1, H2bi
histone H2B.1 A
H2B histone family, member K
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p22.2

Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. The protein has antibacterial and antifungal antimicrobial activity. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]. lac of sum
Disorder MIM:

602807

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_003525 NP_003516 histone H2B type 1-C/E/F/G/I

KEGG
hsa05322 Systemic lupus erythematosus
hsa05203 Viral carcinogenesis
hsa05034 Alcoholism
Reactome
R-HSA-1643685 Disease
R-HSA-977225 Amyloid formation
R-HSA-162582 Signal Transduction
R-HSA-194315 Signaling by Rho GTPases
R-HSA-195258 RHO GTPase Effectors
R-HSA-5625740 RHO GTPases activate PKNs
R-HSA-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
R-HSA-195721 Signaling by Wnt
R-HSA-74160 Gene Expression
R-HSA-73728 RNA Polymerase I Promoter Opening
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-504046 RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
R-HSA-73854 RNA Polymerase I Promoter Clearance
R-HSA-73864 RNA Polymerase I Transcription
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-1640170 Cell Cycle
R-HSA-211000 Gene Silencing by RNA
R-HSA-212165 Epigenetic regulation of gene expression
R-HSA-5250941 Negative epigenetic regulation of rRNA expression
R-HSA-427359 SIRT1 negatively regulates rRNA Expression
R-HSA-5334118 DNA methylation
R-HSA-69278 Cell Cycle, Mitotic
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-68875 Mitotic Prophase
R-HSA-68886 M Phase
R-HSA-2299718 Condensation of Prophase Chromosomes
R-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-HSA-212300 PRC2 methylates histones and DNA
R-HSA-69481 G2/M Checkpoints
R-HSA-69473 G2/M DNA damage checkpoint
R-HSA-1500620 Meiosis
R-HSA-1221632 Meiotic synapsis
R-HSA-157579 Telomere Maintenance
R-HSA-912446 Meiotic recombination
R-HSA-171306 Packaging Of Telomere Ends
R-HSA-73886 Chromosome Maintenance
R-HSA-774815 Nucleosome assembly
R-HSA-2262752 Cellular responses to stress
R-HSA-4839726 Chromatin organization
R-HSA-3247509 Chromatin modifying enzymes
R-HSA-3214847 HATs acetylate histones
R-HSA-2559580 Oxidative Stress Induced Senescence
R-HSA-2559586 DNA Damage/Telomere Stress Induced Senescence
R-HSA-2559583 Cellular Senescence
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-3214815 HDACs deacetylate histones


Homo sapiens (human) HIST1H2BI NP_003516.1
Pan troglodytes (chimpanzee) LOC471888 XP_527266.3
Canis lupus familiaris (dog) LOC100856186 XP_003640209.1
Bos taurus (cattle) LOC616776 XP_002697507.1
Mus musculus (house mouse) Hist1h2bc NP_075911.2
Rattus norvegicus (Norway rat) Hist1h2bh NP_001100822.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HIST1H2BI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIST1H2BI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu30099
NM_003525 Homo sapiens histone cluster 1, H2bi (HIST1H2BI), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu30099
Accession Version NM_003525.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 381bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 18-AUG-2015
Organism Homo sapiens (human)
Product histone H2B type 1-C/E/F/G/I
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z80782.1. On May 24, 2002 this sequence version replaced gi:4504270. Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. The protein has antibacterial and antifungal antimicrobial activity. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 12860195 replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGCCTGAAC CAGCTAAGTC AGCTCCCGCC CCGAAGAAGG GCTCCAAGAA GGCGGTGACC 
AAGGCACAGA AGAAGGATGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTATTCCGTG
TACGTGTACA AGGTGCTGAA GCAGGTCCAC CCCGACACCG GCATCTCGTC CAAGGCTATG
GGGATTATGA ACTCCTTCGT CAACGACATT TTCGAGCGCA TTGCAGGCGA GGCTTCCCGC
CTGGCGCATT ATAACAAGCG CTCGACCATC ACTTCCAGGG AGATCCAAAC GGCTGTGCGC
CTGCTGCTAC CCGGGGAGCT GGCCAAACAC GCGGTGTCGG AGGGCACCAA GGCGGTCACC
AAGTACACCA GCTCCAAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_003516.1
CDS1..381
Misc Feature(1)4..6(+)
Misc Feature(2)16..18(+)
Misc Feature(3)16..18(+)
Misc Feature(4)34..36(+)
Misc Feature(5)34..36(+)
Misc Feature(6)37..39(+)
Misc Feature(7)37..39(+)
Misc Feature(8)43..45(+)
Misc Feature(9)46..48(+)
Misc Feature(10)46..48(+)
Misc Feature(11)49..51(+)
Misc Feature(12)49..51(+)
Misc Feature(13)61..63(+)
Misc Feature(14)61..63(+)
Misc Feature(15)70..72(+)
Misc Feature(16)70..72(+)
Misc Feature(17)82..372(+)
Misc Feature(18)103..105(+)
Misc Feature(19)109..111(+)
Misc Feature(20)139..141(+)
Misc Feature(21)172..174(+)
Misc Feature(22)238..240(+)
Misc Feature(23)256..258(+)
Misc Feature(24)256..258(+)
Misc Feature(25)259..261(+)
Misc Feature(26)277..279(+)
Misc Feature(27)325..327(+)
Misc Feature(28)346..348(+)
Misc Feature(29)349..351(+)
Exon (1)1..437
Gene:HIST1H2BI
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, c dbSNP:772538902
2 2 c, t dbSNP:773536523
3 3 a, c, g, t dbSNP:759220963
5 5 c, g dbSNP:762462418
7 7 c, g dbSNP:776038374
8 8 a, c dbSNP:751560107
10 10 a, c, t dbSNP:145382186
14 14 c, t dbSNP:750192940
20 20 c, t dbSNP:201737810
27 27 a, c, t dbSNP:780356750
28 28 a, g dbSNP:755143837
30 30 c, t dbSNP:778837028
31 31 c, g dbSNP:147739816
33 33 a, g dbSNP:537975664
34 34 a, t dbSNP:375507645
38 38 a, g dbSNP:187127437
40 40 a, g dbSNP:769445418
42 42 c, g dbSNP:201106675
43 43 c, t dbSNP:368315241
44 44 c, g dbSNP:762486317
48 48 c, g dbSNP:768250254
53 53 -, acagaagaa dbSNP:759120123
54 54 -, g dbSNP:776345578
56 56 -, t dbSNP:764781785
57 57 a, c, g dbSNP:200674212
60 60 -, a dbSNP:752417203
63 63 c, g dbSNP:767502992
68 68 -, aga dbSNP:576204357
68 68 a, c dbSNP:750248068
74 74 -, ag dbSNP:763833453
76 76 -, aa dbSNP:751343437
76 76 g, t dbSNP:760613629
78 78 c, t dbSNP:77165086
82 82 a, c dbSNP:754139314
84 84 a, g dbSNP:755126201
87 87 a, g dbSNP:779082787
88 88 a, c, t dbSNP:573050444
89 89 -, g dbSNP:746386504
89 89 g, t dbSNP:758922336
90 90 c, g dbSNP:778190557
93 93 a, g dbSNP:747514210
102 102 c, t dbSNP:772925381
103 103 a, c dbSNP:771330536
104 104 a, c dbSNP:781376245
105 105 a, g dbSNP:748999592
110 110 c, g dbSNP:544696789
116 116 -, c dbSNP:781196392
116 116 c, t dbSNP:773924307
121 121 c, t dbSNP:761159603
123 123 c, t dbSNP:771704074
124 124 c, g dbSNP:558500845
126 126 g, t dbSNP:760668916
127 127 -, g dbSNP:745751112
129 129 c, g dbSNP:575291570
139 139 a, g dbSNP:766317651
141 141 a, g dbSNP:753538230
144 144 a, g dbSNP:759827929
146 146 c, t dbSNP:751642317
147 147 c, t dbSNP:765322240
151 151 c, g dbSNP:752829347
153 153 c, t dbSNP:758545171
154 154 g, t dbSNP:757476038
159 159 c, t dbSNP:777821568
161 161 g, t dbSNP:752096117
162 162 c, t dbSNP:757718972
164 164 -, tc dbSNP:756211276
168 168 g, t dbSNP:781635120
170 170 c, t dbSNP:544359637
171 171 c, t dbSNP:746160029
173 173 a, g dbSNP:754662028
175 175 a, g dbSNP:778495947
179 179 c, t dbSNP:747788870
180 180 a, g dbSNP:771757346
181 181 a, g dbSNP:772818606
183 183 g, t dbSNP:746975839
187 187 a, g dbSNP:770726235
192 192 c, t dbSNP:776501775
194 194 c, g dbSNP:199638887
195 195 -, ctt dbSNP:780069539
197 197 g, t dbSNP:759189359
204 204 a, c, t dbSNP:765079627
207 207 c, t dbSNP:763127270
208 208 a, g dbSNP:764186268
209 209 c, t dbSNP:751586556
216 216 -, gcgcatt dbSNP:749552695
217 217 c, t dbSNP:757772183
221 221 g, t dbSNP:767843779
222 222 c, g, t dbSNP:750935601
237 237 c, g dbSNP:780443801
242 242 c, t dbSNP:530366244
245 245 c, t dbSNP:747843809
246 246 g, t dbSNP:758085217
249 249 c, t dbSNP:149231030
252 252 c, t dbSNP:746462529
255 255 c, t dbSNP:770908365
256 256 a, g dbSNP:200816853
261 261 c, g, t dbSNP:776501537
263 263 c, t dbSNP:769530315
282 282 a, g dbSNP:540774074
287 287 a, g dbSNP:560631520
294 294 g, t dbSNP:61744067
295 295 a, c, g dbSNP:774417051
312 312 c, g dbSNP:767620317
315 315 a, c, g dbSNP:375240174
324 324 c, g dbSNP:766685721
327 327 -, ac dbSNP:768902419
333 333 a, g dbSNP:151290320
342 342 a, g dbSNP:758140378
344 344 g, t dbSNP:777504250
347 347 c, g dbSNP:781421883
353 353 c, g dbSNP:758401818
354 354 a, g dbSNP:369484531
366 366 c, t dbSNP:569267626
370 370 a, g dbSNP:745820739
371 371 c, g dbSNP:769718966
372 372 c, t dbSNP:779834595
377 377 a, g dbSNP:749012763
378 378 a, g dbSNP:371178913
381 381 a, c dbSNP:774612666
390 390 c, t dbSNP:762072729
391 391 c, t dbSNP:772085025
394 394 g, t dbSNP:531587962
397 397 a, t dbSNP:761110290
401 401 c, g dbSNP:766936829
404 404 c, g dbSNP:374791082
407 407 a, g dbSNP:760047601
408 408 a, c, t dbSNP:369741589
410 410 a, g dbSNP:548319946
411 411 c, t dbSNP:373389173
414 414 a, c dbSNP:536044610
416 416 a, g dbSNP:750879995
418 418 c, g dbSNP:749887020
424 424 c, g dbSNP:759342716
426 426 a, g dbSNP:780030896
435 435 c, t dbSNP:553096052

Target ORF information:

RefSeq Version NM_003525
Organism Homo sapiens (human)
Definition Homo sapiens histone cluster 1, H2bi (HIST1H2BI), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003525

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGCCTGAAC CAGCTAAGTC AGCTCCCGCC CCGAAGAAGG GCTCCAAGAA GGCGGTGACC 
AAGGCACAGA AGAAGGATGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTATTCCGTG
TACGTGTACA AGGTGCTGAA GCAGGTCCAC CCCGACACCG GCATCTCGTC CAAGGCTATG
GGGATTATGA ACTCCTTCGT CAACGACATT TTCGAGCGCA TTGCAGGCGA GGCTTCCCGC
CTGGCGCATT ATAACAAGCG CTCGACCATC ACTTCCAGGG AGATCCAAAC GGCTGTGCGC
CTGCTGCTAC CCGGGGAGCT GGCCAAACAC GCGGTGTCGG AGGGCACCAA GGCGGTCACC
AAGTACACCA GCTCCAAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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A proteome-scale map of the human interactome network
Cell 159 (5), 1212-1226 (2014)
Rolland T, Tasan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabasi AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP and Vidal M.


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Flachsbart F, Franke A, Kleindorp R, Caliebe A, Blanche H, Schreiber S and Nebel A.


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MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis
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Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M and Stoorvogel W.


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Marzluff WF, Gongidi P, Woods KR, Jin J and Maltais LJ.


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