×
Home » Species Summary » Homo sapiens » HIST1H4D cDNA ORF clone
Email to GenScript

HIST1H4D cDNA ORF clone, Homo sapiens (human)

Gene Symbol HIST1H4D
Entrez Gene ID 8360
Full Name histone cluster 1, H4d
Synonyms H4/b, H4FB, dJ221C16.9
General protein information
Preferred Names
histone H4
Names
histone H4
histone 1, H4d
H4 histone family, member B
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p22.2

Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]. lac of sum
Disorder MIM:

602823

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_003539 NP_003530 histone H4

KEGG
hsa05322 Systemic lupus erythematosus
hsa05203 Viral carcinogenesis
hsa05034 Alcoholism
Reactome
R-HSA-1643685 Disease
R-HSA-977225 Amyloid formation
R-HSA-162582 Signal Transduction
R-HSA-194315 Signaling by Rho GTPases
R-HSA-195258 RHO GTPase Effectors
R-HSA-5625740 RHO GTPases activate PKNs
R-HSA-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
R-HSA-195721 Signaling by Wnt
R-HSA-74160 Gene Expression
R-HSA-73728 RNA Polymerase I Promoter Opening
R-HSA-504046 RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-73854 RNA Polymerase I Promoter Clearance
R-HSA-73864 RNA Polymerase I Transcription
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-1640170 Cell Cycle
R-HSA-211000 Gene Silencing by RNA
R-HSA-212165 Epigenetic regulation of gene expression
R-HSA-5250941 Negative epigenetic regulation of rRNA expression
R-HSA-427359 SIRT1 negatively regulates rRNA Expression
R-HSA-5334118 DNA methylation
R-HSA-69278 Cell Cycle, Mitotic
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-68875 Mitotic Prophase
R-HSA-68886 M Phase
R-HSA-2299718 Condensation of Prophase Chromosomes
R-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-HSA-212300 PRC2 methylates histones and DNA
R-HSA-69481 G2/M Checkpoints
R-HSA-69473 G2/M DNA damage checkpoint
R-HSA-1500620 Meiosis
R-HSA-1221632 Meiotic synapsis
R-HSA-157579 Telomere Maintenance
R-HSA-912446 Meiotic recombination
R-HSA-171306 Packaging Of Telomere Ends
R-HSA-73886 Chromosome Maintenance
R-HSA-774815 Nucleosome assembly
R-HSA-2262752 Cellular responses to stress
R-HSA-4839726 Chromatin organization
R-HSA-3247509 Chromatin modifying enzymes
R-HSA-3214847 HATs acetylate histones
R-HSA-2559580 Oxidative Stress Induced Senescence
R-HSA-2559586 DNA Damage/Telomere Stress Induced Senescence
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-2559583 Cellular Senescence
R-HSA-3214842 HDMs demethylate histones
R-HSA-3214841 PKMTs methylate histone lysines
R-HSA-3214815 HDACs deacetylate histones
R-HSA-3214858 RMTs methylate histone arginines
Pathway Interaction Database
hdac_classiii_pathway Signaling events mediated by HDAC Class III
WikiPathways
WP619 Type II interferon signaling (IFNG)
WP2361 Gastric cancer network 1


Homo sapiens (human) HIST1H4D NP_003530.1
Pan troglodytes (chimpanzee) LOC748513 XP_001172726.1
Macaca mulatta (Rhesus monkey) LOC704341 XP_001095996.1
Canis lupus familiaris (dog) LOC100856225 XP_003640212.1
Bos taurus (cattle) LOC100337407 XP_005226918.1
Mus musculus (house mouse) Hist1h4m NP_001182350.1
Rattus norvegicus (Norway rat) Hist2h4a XP_578415.2
Gallus gallus (chicken) HIST1H46L2 XP_003640416.1
Danio rerio (zebrafish) LOC100002181 XP_001336407.1
Drosophila melanogaster (fruit fly) His4:CG33891 NP_001027327.1
Caenorhabditis elegans his-60 NP_501203.2
Xenopus (Silurana) tropicalis (western clawed frog) LOC100495739 XP_002933940.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HIST1H4D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIST1H4D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu30175 NM_003539 Homo sapiens histone cluster 1, H4d (HIST1H4D), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu30175
Accession Version NM_003539.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 312bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-AUG-2015
Organism Homo sapiens (human)
Product histone H4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X60482.1. On May 22, 2002 this sequence version replaced gi:5579463. Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: CA406688.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGTCTGGCC GCGGTAAGGG CGGAAAGGGT CTAGGTAAGG GTGGCGCCAA GCGTCACCGT 
AAGGTATTGC GTGACAATAT CCAAGGAATC ACCAAGCCCG CTATCCGCCG CCTGGCTCGC
CGCGGCGGCG TCAAGCGTAT TTCTGGCCTC ATTTATGAGG AAACTCGCGG AGTGCTGAAA
GTTTTCCTGG AAAATGTAAT CCGCGATGCT GTCACCTACA CGGAACACGC CAAACGCAAG
ACAGTCACAG CCATGGACGT GGTGTACGCG CTCAAGCGCC AGGGACGCAC TCTTTATGGC
TTCGGCGGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_003530.1
CDS1..312
Misc Feature(1)1..309(+)
Misc Feature(2)4..6(+)
Misc Feature(3)4..6(+)
Misc Feature(4)10..12(+)
Misc Feature(5)10..12(+)
Misc Feature(6)16..18(+)
Misc Feature(7)16..18(+)
Misc Feature(8)25..27(+)
Misc Feature(9)25..27(+)
Misc Feature(10)37..39(+)
Misc Feature(11)37..39(+)
Misc Feature(12)49..51(+)
Misc Feature(13)49..51(+)
Misc Feature(14)52..303(+)
Misc Feature(15)61..63(+)
Misc Feature(16)94..96(+)
Misc Feature(17)100..273(+)
Misc Feature(18)121..135(+)
Misc Feature(19)133..291(+)
Misc Feature(20)142..144(+)
Misc Feature(21)154..156(+)
Misc Feature(22)205..279(+)
Misc Feature(23)265..267(+)
Misc Feature(24)274..276(+)
Misc Feature(25)274..276(+)
Misc Feature(26)289..303(+)
Exon (1)1..367
Gene:HIST1H4D
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, c, g dbSNP:200821779
2 2 a, t dbSNP:149993334
3 3 a, g dbSNP:747940205
5 5 c, g, t dbSNP:201613383
7 7 a, g dbSNP:749175349
8 8 a, c, g, t dbSNP:749934539
9 9 a, c, g, t dbSNP:199624269
10 10 c, g, t dbSNP:140430462
11 11 a, g dbSNP:754328775
12 12 a, c, g, t dbSNP:560226680
14 14 g, t dbSNP:539823981
16 16 a, g dbSNP:774402895
19 19 a, g dbSNP:574527741
21 21 c, g, t dbSNP:151274627
24 24 a, g, t dbSNP:769708257
27 27 a, g dbSNP:142303259
28 28 a, g dbSNP:780709577
29 29 a, g dbSNP:368566658
31 31 c, t dbSNP:554616183
32 32 c, t dbSNP:779146331
33 33 a, t dbSNP:139257264
35 35 a, g dbSNP:754242447
36 36 g, t dbSNP:767039326
39 39 c, g dbSNP:756656965
41 41 -, g dbSNP:761193902
45 45 c, g, t dbSNP:150838709
46 46 -, g dbSNP:762079534
48 48 c, g, t dbSNP:375888188
54 54 c, g, t dbSNP:146500115
57 57 c, t dbSNP:762799179
59 59 a, g dbSNP:570837491
63 63 a, c, g dbSNP:200441194
64 64 c, g dbSNP:745766433
65 65 c, t dbSNP:776014318
70 70 c, g dbSNP:770403660
71 71 a, g dbSNP:746496736
75 75 c, t dbSNP:777538918
76 76 a, g dbSNP:758099718
78 78 c, t dbSNP:201162303
79 79 a, g dbSNP:780528340
80 80 c, g, t dbSNP:750884941
81 81 -, t dbSNP:768846426
81 81 c, g dbSNP:767500097
82 82 c, g, t dbSNP:751583245
86 86 a, g, t dbSNP:368091679
88 88 a, g dbSNP:763133612
90 90 c, t dbSNP:144085716
94 94 a, c dbSNP:202049591
96 96 a, g dbSNP:548178202
98 98 c, t dbSNP:201894000
99 99 c, t dbSNP:73736557
100 100 a, g dbSNP:776694850
104 104 c, t dbSNP:569549632
105 105 c, t dbSNP:746577882
108 108 c, t dbSNP:772816360
112 112 c, t dbSNP:552478518
113 113 a, t dbSNP:747755990
114 114 a, g dbSNP:146262007
117 117 c, t dbSNP:762893570
118 118 c, t dbSNP:773088974
120 120 c, t dbSNP:756481227
123 123 c, g dbSNP:746214961
126 126 c, t dbSNP:781753823
129 129 c, t dbSNP:757687288
133 133 a, t dbSNP:373964325
135 135 a, g dbSNP:371511135
143 143 c, g, t dbSNP:752846007
145 145 a, g dbSNP:765437973
146 146 c, g dbSNP:759432453
147 147 c, g, t dbSNP:766377500
150 150 c, g dbSNP:760487699
156 156 g, t dbSNP:772871879
157 157 g, t dbSNP:765008574
159 159 a, c, g dbSNP:201318128
165 165 -, t dbSNP:779259978
165 165 c, t dbSNP:774215046
166 166 a, c, t dbSNP:369583517
167 167 a, g dbSNP:746297564
168 168 a, c, t dbSNP:771503236
174 174 a, g dbSNP:747454273
175 175 -, ctg dbSNP:763205100
175 175 c, g dbSNP:368348095
177 177 g, t dbSNP:758536923
183 183 c, t dbSNP:752756387
186 186 c, t dbSNP:761302691
187 187 c, t dbSNP:755124179
188 188 c, t dbSNP:753696049
192 192 a, c, g dbSNP:760676646
197 197 c, t dbSNP:750321677
200 200 c, t dbSNP:149672595
201 201 c, t dbSNP:139439104
202 202 c, t dbSNP:544244183
208 208 a, g dbSNP:768397391
209 209 c, t dbSNP:762477789
213 213 c, t dbSNP:777079811
214 214 a, t dbSNP:771130609
216 216 c, g, t dbSNP:778307288
219 219 c, t dbSNP:772545434
221 221 c, t dbSNP:374211503
222 222 a, c, g, t dbSNP:755249966
225 225 a, g dbSNP:749414627
230 230 c, t dbSNP:779904879
231 231 c, t dbSNP:755931857
235 235 c, g dbSNP:750231768
236 236 g, t dbSNP:150538742
240 240 a, g dbSNP:757232898
242 242 c, g, t dbSNP:763466461
243 243 a, g dbSNP:141704151
246 246 c, t dbSNP:111343206
247 247 a, g dbSNP:764927627
248 248 c, t dbSNP:144742137
250 250 a, g dbSNP:573032310
252 252 c, t dbSNP:772596789
253 253 a, g dbSNP:748605608
259 259 a, g dbSNP:774501028
264 264 a, g dbSNP:768553063
267 267 c, t dbSNP:749381779
270 270 a, c, g, t dbSNP:139559138
273 273 c, t dbSNP:781096819
279 279 c, g dbSNP:371070395
282 282 a, g dbSNP:757286384
285 285 a, t dbSNP:751398886
286 286 c, g dbSNP:764084178
288 288 c, t dbSNP:757899947
292 292 c, t dbSNP:752226419
294 294 -, tt dbSNP:775510690
294 294 c, g, t dbSNP:759147206
296 296 -, at dbSNP:769790720
297 297 c, t dbSNP:552898923
307 307 g, t dbSNP:767811249
310 310 -, t dbSNP:769028855
311 311 c, g, t dbSNP:774787495
313 313 a, c, g, t dbSNP:376927129
314 314 c, g dbSNP:770173337
316 316 a, t dbSNP:746042680
318 318 a, c, t dbSNP:374437133
321 321 c, t dbSNP:370199061
322 322 -, ct dbSNP:780982962
323 323 a, c, g dbSNP:768107645
324 324 c, t dbSNP:557107958
325 325 a, g dbSNP:201191728
328 328 a, g dbSNP:778343999
329 329 c, t dbSNP:754638324
330 330 a, g dbSNP:753421131
334 334 c, g dbSNP:766017477
336 336 a, g dbSNP:762142778
344 344 -, aac dbSNP:768430876
344 344 a, g dbSNP:775923684
345 345 a, g dbSNP:751932536
346 346 c, t dbSNP:764549806
349 349 c, t dbSNP:574611429
351 351 a, c, g, t dbSNP:373810094
352 352 a, t dbSNP:759845537
356 356 a, c dbSNP:777048425
362 362 -, c dbSNP:746775485
362 362 c, t dbSNP:771252307
363 363 a, c, g dbSNP:777581565

Target ORF information:

RefSeq Version NM_003539
Organism Homo sapiens (human)
Definition Homo sapiens histone cluster 1, H4d (HIST1H4D), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003539

ORF Insert Sequence:

1
61
121
181
241
301
ATGTCTGGCC GCGGTAAGGG CGGAAAGGGT CTAGGTAAGG GTGGCGCCAA GCGTCACCGT 
AAGGTATTGC GTGACAATAT CCAAGGAATC ACCAAGCCCG CTATCCGCCG CCTGGCTCGC
CGCGGCGGCG TCAAGCGTAT TTCTGGCCTC ATTTATGAGG AAACTCGCGG AGTGCTGAAA
GTTTTCCTGG AAAATGTAAT CCGCGATGCT GTCACCTACA CGGAACACGC CAAACGCAAG
ACAGTCACAG CCATGGACGT GGTGTACGCG CTCAAGCGCC AGGGACGCAC TCTTTATGGC
TTCGGCGGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Human tNASP promotes in vitro nucleosome assembly with histone H3.3
Biochemistry 54 (5), 1171-1179 (2015)
Kato D, Osakabe A, Tachiwana H, Tanaka H and Kurumizaka H.


book

A proteome-scale map of the human interactome network
Cell 159 (5), 1212-1226 (2014)
Rolland T, Tasan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabasi AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP and Vidal M.


book

Structural insights into recognition of acetylated histone ligands by the BRPF1 bromodomain
FEBS Lett. 588 (21), 3844-3854 (2014)
Lubula MY, Eckenroth BE, Carlson S, Poplawski A, Chruszcz M and Glass KC.


book

Molecular functions of the TLE tetramerization domain in Wnt target gene repression
EMBO J. 33 (7), 719-731 (2014)
Chodaparambil JV, Pate KT, Hepler MR, Tsai BP, Muthurajan UM, Luger K, Waterman ML and Weis WI.


book

Structure of human nucleosome containing the testis-specific histone variant TSH2B
Acta Crystallogr F Struct Biol Commun 70 (PT 4), 444-449 (2014)
Urahama T, Horikoshi N, Osakabe A, Tachiwana H and Kurumizaka H.


book

The human and mouse replication-dependent histone genes
Genomics 80 (5), 487-498 (2002)
Marzluff WF, Gongidi P, Woods KR, Jin J and Maltais LJ.


book

The integrated activities of IRF-2 (HiNF-M), CDP/cut (HiNF-D) and H4TF-2 (HiNF-P) regulate transcription of a cell cycle controlled human histone H4 gene: mechanistic differences between distinct H4 genes
Mol. Biol. Rep. 25 (1), 1-12 (1998)
Aziz F, van Wijnen AJ, Vaughan PS, Wu S, Shakoori AR, Lian JB, Soprano KJ, Stein JL and Stein GS.


book

The human histone gene cluster at the D6S105 locus
Hum. Genet. 101 (3), 284-294 (1997)
Albig W and Doenecke D.


book

Human histone gene organization: nonregular arrangement within a large cluster
Genomics 40 (2), 314-322 (1997)
Albig W, Kioschis P, Poustka A, Meergans K and Doenecke D.


book

Protein-DNA interactions in vivo upstream of a cell cycle-regulated human H4 histone gene
Science 236 (4806), 1308-1311 (1987)
Pauli,U., Chrysogelos,S., Stein,G., Stein,J. and Nick,H.


 
*
*
*
*