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HIST1H4B cDNA ORF clone, Homo sapiens (human)

Gene Symbol HIST1H4B
Entrez Gene ID 8366
Full Name histone cluster 1, H4b
Synonyms H4/I, H4FI
General protein information
Preferred Names
histone H4
Names
histone H4
histone 1, H4b
H4 histone family, member I
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p22.2

Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]. lac of sum
Disorder MIM:

602829


mRNA and Protein(s)

mRNA Protein Name
NM_003544 NP_003535 histone H4

KEGG
hsa05322 Systemic lupus erythematosus
hsa05203 Viral carcinogenesis
hsa05034 Alcoholism
Reactome
R-HSA-1643685 Disease
R-HSA-977225 Amyloid formation
R-HSA-162582 Signal Transduction
R-HSA-194315 Signaling by Rho GTPases
R-HSA-195258 RHO GTPase Effectors
R-HSA-5625740 RHO GTPases activate PKNs
R-HSA-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
R-HSA-195721 Signaling by Wnt
R-HSA-74160 Gene Expression
R-HSA-73728 RNA Polymerase I Promoter Opening
R-HSA-504046 RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-73854 RNA Polymerase I Promoter Clearance
R-HSA-73864 RNA Polymerase I Transcription
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-1640170 Cell Cycle
R-HSA-211000 Gene Silencing by RNA
R-HSA-212165 Epigenetic regulation of gene expression
R-HSA-5250941 Negative epigenetic regulation of rRNA expression
R-HSA-427359 SIRT1 negatively regulates rRNA Expression
R-HSA-5334118 DNA methylation
R-HSA-69278 Cell Cycle, Mitotic
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-68875 Mitotic Prophase
R-HSA-68886 M Phase
R-HSA-2299718 Condensation of Prophase Chromosomes
R-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-HSA-212300 PRC2 methylates histones and DNA
R-HSA-69481 G2/M Checkpoints
R-HSA-69473 G2/M DNA damage checkpoint
R-HSA-1500620 Meiosis
R-HSA-1221632 Meiotic synapsis
R-HSA-157579 Telomere Maintenance
R-HSA-912446 Meiotic recombination
R-HSA-171306 Packaging Of Telomere Ends
R-HSA-73886 Chromosome Maintenance
R-HSA-774815 Nucleosome assembly
R-HSA-2262752 Cellular responses to stress
R-HSA-4839726 Chromatin organization
R-HSA-3247509 Chromatin modifying enzymes
R-HSA-3214847 HATs acetylate histones
R-HSA-2559580 Oxidative Stress Induced Senescence
R-HSA-2559586 DNA Damage/Telomere Stress Induced Senescence
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-2559583 Cellular Senescence
R-HSA-3214842 HDMs demethylate histones
R-HSA-3214841 PKMTs methylate histone lysines
R-HSA-3214815 HDACs deacetylate histones
R-HSA-3214858 RMTs methylate histone arginines
Pathway Interaction Database
hdac_classiii_pathway Signaling events mediated by HDAC Class III
WikiPathways
WP619 Type II interferon signaling (IFNG)
WP2361 Gastric cancer network 1


Homo sapiens (human) HIST1H4B NP_003535.1
Pan troglodytes (chimpanzee) LOC462507 XP_518300.2
Bos taurus (cattle) LOC529647 XP_005223853.1
Mus musculus (house mouse) Hist4h4 NP_783583.1
Rattus norvegicus (Norway rat) Hist2h4 NP_001116941.1
Gallus gallus (chicken) HIST1H46L3 XP_004937726.1
Danio rerio (zebrafish) LOC100332668 XP_002666964.1
Drosophila melanogaster (fruit fly) His4:CG33871 NP_001027377.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100496758 XP_002943144.1

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HIST1H4B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIST1H4B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu30365
NM_003544 Homo sapiens histone cluster 1, H4b (HIST1H4B), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $49.50
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu30365
Clone ID Related Accession (Same CDS sequence) NM_003544
Accession Version NM_003544.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 312bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 18-AUG-2015
Organism Homo sapiens (human)
Product histone H4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X67081.1. On May 24, 2002 this sequence version replaced gi:4504312. Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC069467.1, DB027810.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGTCTGGTC GCGGCAAAGG CGGTAAAGGT TTGGGTAAGG GAGGTGCCAA GCGTCACCGA 
AAAGTGCTGC GGGATAACAT CCAAGGCATC ACCAAACCGG CCATTCGGCG CCTTGCTAGG
CGTGGTGGGG TTAAGCGAAT TTCCGGTTTG ATTTATGAGG AGACTCGTGG CGTTCTCAAG
GTGTTTCTGG AGAACGTGAT CCGGGACGCC GTGACCTACA CGGAGCACGC CAAGCGCAAG
ACTGTCACTG CCATGGATGT GGTTTACGCG CTCAAGCGTC AAGGACGCAC TCTGTACGGC
TTCGGCGGTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003535.1
CDS1..312
Misc Feature(1)1..309(+)
Misc Feature(2)4..6(+)
Misc Feature(3)4..6(+)
Misc Feature(4)10..12(+)
Misc Feature(5)10..12(+)
Misc Feature(6)16..18(+)
Misc Feature(7)16..18(+)
Misc Feature(8)25..27(+)
Misc Feature(9)25..27(+)
Misc Feature(10)37..39(+)
Misc Feature(11)37..39(+)
Misc Feature(12)49..51(+)
Misc Feature(13)49..51(+)
Misc Feature(14)52..303(+)
Misc Feature(15)61..63(+)
Misc Feature(16)94..96(+)
Misc Feature(17)100..273(+)
Misc Feature(18)121..135(+)
Misc Feature(19)133..291(+)
Misc Feature(20)142..144(+)
Misc Feature(21)154..156(+)
Misc Feature(22)205..279(+)
Misc Feature(23)265..267(+)
Misc Feature(24)274..276(+)
Misc Feature(25)274..276(+)
Misc Feature(26)289..303(+)
Exon (1)1..357
Gene:HIST1H4B
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, c, g, t dbSNP:139550856
2 2 c, t dbSNP:150979745
3 3 a, g dbSNP:201721390
4 4 a, g, t dbSNP:766341481
5 5 a, c, t dbSNP:142718461
6 6 a, c, t dbSNP:116171042
7 7 c, g dbSNP:761634379
10 10 -, t dbSNP:778342743
10 10 c, t dbSNP:774444664
11 11 a, c, g dbSNP:749571332
12 12 c, g, t dbSNP:569686893
13 13 -, cgcg dbSNP:772497375
13 13 a, c, g dbSNP:745592288
15 15 -, caaaggcgg dbSNP:779168790
15 15 -, t dbSNP:556617477
15 15 c, t dbSNP:776257737
16 16 a, c dbSNP:772427705
17 17 a, g dbSNP:748452381
19 19 a, g, t dbSNP:141485038
20 20 c, g dbSNP:560733180
21 21 c, t dbSNP:28688702
23 23 a, g dbSNP:756144966
24 24 -, t dbSNP:535221714
24 24 c, t dbSNP:145960112
25 25 a, g dbSNP:750679477
29 29 a, g dbSNP:777969227
30 30 g, t dbSNP:767791486
32 32 g, t dbSNP:757142938
33 33 a, g dbSNP:751358482
35 35 a, g, t dbSNP:762996462
37 37 a, g dbSNP:775276464
40 40 a, g dbSNP:764864503
41 41 a, g dbSNP:758900343
42 42 a, g dbSNP:776276032
43 43 a, g dbSNP:770519122
44 44 a, g dbSNP:183253971
45 45 c, t dbSNP:774749592
47 47 a, c dbSNP:768892611
48 48 a, c, g, t dbSNP:3752419
50 50 -, aa dbSNP:780227319
51 51 a, g dbSNP:745895525
52 52 c, t dbSNP:781597714
53 53 a, g dbSNP:757590972
54 54 a, t dbSNP:201649393
55 55 c, t dbSNP:751808630
56 56 a, g dbSNP:764006473
57 57 c, g, t dbSNP:752680111
58 58 c, g, t dbSNP:199612297
59 59 a, g dbSNP:776160725
60 60 a, g dbSNP:765855772
61 61 a, g dbSNP:760381892
63 63 a, g dbSNP:772740180
66 66 a, g dbSNP:769069850
67 67 c, g, t dbSNP:776087679
70 70 c, t dbSNP:770161997
71 71 a, g dbSNP:746344147
72 72 a, c, g dbSNP:748119687
75 75 c, t dbSNP:747300944
78 78 c, t dbSNP:750506383
81 81 c, t dbSNP:758223178
82 82 c, t dbSNP:201003635
85 85 a, g dbSNP:778937950
87 87 c, t dbSNP:150244822
88 88 a, g dbSNP:753765789
92 92 c, g dbSNP:765944150
93 93 c, g, t dbSNP:142030763
96 96 a, g dbSNP:372902403
97 97 c, t dbSNP:267600908
99 99 a, c, g, t dbSNP:760176610
101 101 c, t dbSNP:200478043
102 102 c, t dbSNP:200226920
103 103 a, c, t dbSNP:778075262
106 106 c, t dbSNP:772142519
107 107 a, g dbSNP:748382526
111 111 c, t dbSNP:138516170
115 115 a, g dbSNP:754767006
116 116 c, t dbSNP:754834973
117 117 c, t dbSNP:779908370
120 120 -, tagg dbSNP:756248336
124 124 c, g dbSNP:755644348
129 129 a, g dbSNP:749861454
134 134 -, a dbSNP:750533082
136 136 c, t dbSNP:767025164
138 138 a, g dbSNP:147037406
143 143 a, c dbSNP:751210165
144 144 c, t dbSNP:141516292
145 145 g, t dbSNP:759912819
147 147 c, t dbSNP:777285966
150 150 a, g dbSNP:369387838
152 152 c, t dbSNP:766940867
155 155 -, at dbSNP:767458667
155 155 a, g dbSNP:375239146
156 156 c, t dbSNP:773201997
162 162 c, g dbSNP:147907725
163 163 a, g dbSNP:143307191
164 164 c, g, t dbSNP:149158970
165 165 a, t dbSNP:768453578
166 166 a, c, g, t dbSNP:553183619
168 168 c, t dbSNP:145034636
170 170 a, g dbSNP:751486416
171 171 c, t dbSNP:756783566
172 172 c, g dbSNP:751172182
173 173 -, cgtggcgt dbSNP:757189352
174 174 c, t dbSNP:367765013
175 175 c, t dbSNP:202202685
176 176 g, t dbSNP:201938912
177 177 c, t dbSNP:766818304
180 180 a, g dbSNP:369587645
181 181 a, g dbSNP:773766887
182 182 c, t dbSNP:780045847
183 183 a, g dbSNP:143317609
186 186 c, t dbSNP:774582253
187 187 c, t dbSNP:768830220
188 188 g, t dbSNP:749485045
189 189 a, g dbSNP:775316240
190 190 a, g dbSNP:139563717
192 192 a, g dbSNP:769615653
194 194 a, g dbSNP:745857495
195 195 c, g, t dbSNP:115249469
197 197 c, g, t dbSNP:372438348
198 198 a, g dbSNP:192365376
199 199 a, g dbSNP:777598662
200 200 g, t dbSNP:758163186
201 201 c, t dbSNP:143186972
202 202 c, t dbSNP:766918749
205 205 g, t dbSNP:565829180
207 207 c, t dbSNP:756435021
208 208 c, g dbSNP:750908015
209 209 c, g, t dbSNP:750164351
210 210 c, t dbSNP:761834115
212 212 a, t dbSNP:751682407
213 213 -, tg dbSNP:751316629
213 213 g, t dbSNP:764139318
215 215 c, t dbSNP:763220667
216 216 c, t dbSNP:139832518
218 218 -, a dbSNP:763903729
219 219 c, t dbSNP:769477226
221 221 c, t dbSNP:367858628
222 222 a, g dbSNP:776451272
229 229 c, g dbSNP:770861467
230 230 c, t dbSNP:552641204
235 235 c, t dbSNP:777473620
236 236 a, c, g dbSNP:374317204
237 237 c, t dbSNP:778571737
239 239 a, g dbSNP:756457842
241 241 -, t dbSNP:762524292
241 241 a, g dbSNP:750764275
242 242 c, t dbSNP:781384208
243 243 -, t dbSNP:775133981
244 244 -, gt dbSNP:763713647
244 244 a, c, g dbSNP:752050927
246 246 c, t dbSNP:370581694
247 247 a, g dbSNP:762951010
248 248 c, t dbSNP:150504596
250 250 a, g dbSNP:532818487
251 251 -, ccatggatg dbSNP:776057506
251 251 c, t dbSNP:765446822
252 252 c, t dbSNP:200017457
253 253 a, g dbSNP:546755781
254 254 c, t dbSNP:368047810
263 263 c, t dbSNP:770609260
267 267 c, t dbSNP:116366733
269 269 c, t dbSNP:148427340
270 270 a, g dbSNP:771722616
271 271 -, gc dbSNP:772585979
273 273 c, t dbSNP:747613531
276 276 a, g dbSNP:753237740
279 279 g, t dbSNP:150550889
286 286 c, t dbSNP:748874128
287 287 a, g dbSNP:781665075
288 288 c, t dbSNP:757616770
289 289 a, c dbSNP:752063375
291 291 c, t dbSNP:778192629
294 294 -, gt dbSNP:769738200
294 294 a, g dbSNP:567364267
297 297 c, t dbSNP:752778199
298 298 a, g dbSNP:765199777
300 300 c, t dbSNP:368805598
303 303 c, t dbSNP:149602824
306 306 c, t dbSNP:182575157
308 308 -, g dbSNP:146741139
309 309 c, g, t dbSNP:773305848
312 312 -, ggt dbSNP:769158572
313 313 -, g dbSNP:749729179
314 314 c, t dbSNP:767521768
315 315 -, ga dbSNP:780418095
315 315 c, t dbSNP:761815984
319 319 c, t dbSNP:773963446
320 320 a, c, g dbSNP:369368546
320 320 -, g dbSNP:756442624
321 321 c, t dbSNP:774936561
322 322 -, cac dbSNP:745938020
323 323 a, g dbSNP:370987576
324 324 -, ct dbSNP:781152613
324 324 g, t dbSNP:200859082
325 325 -, gca dbSNP:757174503
328 328 c, t dbSNP:778278819
329 329 a, c dbSNP:377616414
332 332 c, t dbSNP:771353082
333 333 c, t dbSNP:373534445
334 334 a, c, g dbSNP:754106567
335 335 a, g dbSNP:371496317
336 336 g, t dbSNP:756004264
338 338 a, g, t dbSNP:376264919
339 339 c, t dbSNP:761872377
340 340 c, t dbSNP:114259939
341 341 c, t dbSNP:763668023
346 346 c, t dbSNP:3752420
352 352 c, t dbSNP:775228369
353 353 a, g, t dbSNP:745486218
355 355 c, t dbSNP:773614319
357 357 a, t dbSNP:772411469

Target ORF information:

RefSeq Version NM_003544
Organism Homo sapiens (human)
Definition Homo sapiens histone cluster 1, H4b (HIST1H4B), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003544

ORF Insert Sequence:

1
61
121
181
241
301
ATGTCTGGTC GCGGCAAAGG CGGTAAAGGT TTGGGTAAGG GAGGTGCCAA GCGTCACCGA 
AAAGTGCTGC GGGATAACAT CCAAGGCATC ACCAAACCGG CCATTCGGCG CCTTGCTAGG
CGTGGTGGGG TTAAGCGAAT TTCCGGTTTG ATTTATGAGG AGACTCGTGG CGTTCTCAAG
GTGTTTCTGG AGAACGTGAT CCGGGACGCC GTGACCTACA CGGAGCACGC CAAGCGCAAG
ACTGTCACTG CCATGGATGT GGTTTACGCG CTCAAGCGTC AAGGACGCAC TCTGTACGGC
TTCGGCGGTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Human tNASP promotes in vitro nucleosome assembly with histone H3.3
Biochemistry 54 (5), 1171-1179 (2015)
Kato D, Osakabe A, Tachiwana H, Tanaka H and Kurumizaka H.


book

A proteome-scale map of the human interactome network
Cell 159 (5), 1212-1226 (2014)
Rolland T, Tasan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabasi AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP and Vidal M.


book

Structural insights into recognition of acetylated histone ligands by the BRPF1 bromodomain
FEBS Lett. 588 (21), 3844-3854 (2014)
Lubula MY, Eckenroth BE, Carlson S, Poplawski A, Chruszcz M and Glass KC.


book

Molecular functions of the TLE tetramerization domain in Wnt target gene repression
EMBO J. 33 (7), 719-731 (2014)
Chodaparambil JV, Pate KT, Hepler MR, Tsai BP, Muthurajan UM, Luger K, Waterman ML and Weis WI.


book

Structure of human nucleosome containing the testis-specific histone variant TSH2B
Acta Crystallogr F Struct Biol Commun 70 (PT 4), 444-449 (2014)
Urahama T, Horikoshi N, Osakabe A, Tachiwana H and Kurumizaka H.


book

The human and mouse replication-dependent histone genes
Genomics 80 (5), 487-498 (2002)
Marzluff WF, Gongidi P, Woods KR, Jin J and Maltais LJ.


book

A 1.1-Mb transcript map of the hereditary hemochromatosis locus
Genome Res. 7 (5), 441-456 (1997)
Ruddy DA, Kronmal GS, Lee VK, Mintier GA, Quintana L, Domingo R Jr, Meyer NC, Irrinki A, McClelland EE, Fullan A, Mapa FA, Moore T, Thomas W, Loeb DB, Harmon C, Tsuchihashi Z, Wolff RK, Schatzman RC and Feder JN.


book

Human histone gene organization: nonregular arrangement within a large cluster
Genomics 40 (2), 314-322 (1997)
Albig W, Kioschis P, Poustka A, Meergans K and Doenecke D.


book

Isolation and characterization of two human H1 histone genes within clusters of core histone genes
Genomics 10 (4), 940-948 (1991)
Albig W, Kardalinou E, Drabent B, Zimmer A and Doenecke D.


book

Protein-DNA interactions in vivo upstream of a cell cycle-regulated human H4 histone gene
Science 236 (4806), 1308-1311 (1987)
Pauli,U., Chrysogelos,S., Stein,G., Stein,J. and Nick,H.


 
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