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MAGT1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAGT1
Entrez Gene ID 84061
Full Name magnesium transporter 1
Synonyms IAP, MRX95, OST3B, PRO0756, XMEN, bA217H1.1
General protein information
Preferred Names
magnesium transporter protein 1
Names
magnesium transporter protein 1
implantation-associated protein
oligosaccharyltransferase 3 homolog B
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq21.1

Summary This gene encodes a magnesium cation transporter protein that localizes to the cell membrane. This protein also associates with N-oligosaccharyl transferase and therefore may have a role in N-glycosylation. Mutations in this gene cause mental retardation X-linked type 95 (MRX95). This gene may have multiple in-frame translation initiation sites, one of which would encode a shorter protein with an N-terminus containing a signal peptide at amino acids 1-29. [provided by RefSeq, Jan 2010]. lac of sum
Disorder MIM:

300715

Disorder Html: Mental retardation, X-linked 95, 300716 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_032121 NP_115497 magnesium transporter protein 1

Reactome
R-HSA-392499 Metabolism of proteins
R-HSA-446203 Asparagine N-linked glycosylation
R-HSA-597592 Post-translational protein modification
R-HSA-5223345 Miscellaneous transport and binding events
R-HSA-382551 Transmembrane transport of small molecules


Homo sapiens (human) MAGT1 NP_115497.4
Macaca mulatta (Rhesus monkey) LOC710098 XP_002806337.1
Canis lupus familiaris (dog) MAGT1 XP_549095.3
Bos taurus (cattle) MAGT1 NP_001231247.1
Mus musculus (house mouse) Magt1 NP_001177338.1
Rattus norvegicus (Norway rat) Magt1 NP_446398.1
Gallus gallus (chicken) MAGT1 NP_001006435.1
Danio rerio (zebrafish) magt1 NP_955994.1
Xenopus (Silurana) tropicalis (western clawed frog) magt1 XP_002931857.1

Component

ID Name Evidence
GO:0005783 endoplasmic reticulum IEA
GO:0008250 oligosaccharyltransferase complex IDA
GO:0016020 membrane IEA
GO:0016021 integral to membrane IEA

Function

ID Name Evidence
GO:0004579 dolichyl-diphosphooligosaccharide-protein glycotransferase activity TAS

Process

ID Name Evidence
GO:0018279 protein N-linked glycosylation via asparagine TAS

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAGT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18683
NM_032121 Homo sapiens magnesium transporter 1 (MAGT1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $159.50
$319.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu18683
Accession Version NM_032121.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1104bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product magnesium transporter protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC329979.1, BC060842.1 and BC041014.1. This sequence is a reference standard in the RefSeqGene project. On Dec 5, 2008 this sequence version replaced gi:141801933. Summary: This gene encodes a magnesium cation transporter protein that localizes to the cell membrane. This protein also associates with N-oligosaccharyl transferase and therefore may have a role in N-glycosylation. Mutations in this gene cause mental retardation X-linked type 95 (MRX95). This gene may have multiple in-frame translation initiation sites, one of which would encode a shorter protein with an N-terminus containing a signal peptide at amino acids 1-29. [provided by RefSeq, Jan 2010]. ##Evidence-Data-START## Transcript exon combination :: BC060842.1, BC041014.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGGAAAG GCAAGGGGCC GATTTGCCTG TTCTCACGCC CCACCCTCAG ACCTAGCCGG 
AGCAAAGTTT CACTTATAGA AGGGAGAGGA GCGAACATGG CAGCGCGTTG GCGGTTTTGG
TGTGTCTCTG TGACCATGGT GGTGGCGCTG CTCATCGTTT GCGACGTTCC CTCAGCCTCT
GCCCAAAGAA AGAAGGAGAT GGTGTTATCT GAAAAGGTTA GTCAGCTGAT GGAATGGACT
AACAAAAGAC CTGTAATAAG AATGAATGGA GACAAGTTCC GTCGCCTTGT GAAAGCCCCA
CCGAGAAATT ACTCCGTTAT CGTCATGTTC ACTGCTCTCC AACTGCATAG ACAGTGTGTC
GTTTGCAAGC AAGCTGATGA AGAATTCCAG ATCCTGGCAA ACTCCTGGCG ATACTCCAGT
GCATTCACCA ACAGGATATT TTTTGCCATG GTGGATTTTG ATGAAGGCTC TGATGTATTT
CAGATGCTAA ACATGAATTC AGCTCCAACT TTCATCAACT TTCCTGCAAA AGGGAAACCC
AAACGGGGTG ATACATATGA GTTACAGGTG CGGGGTTTTT CAGCTGAGCA GATTGCCCGG
TGGATCGCCG ACAGAACTGA TGTCAATATT AGAGTGATTA GACCCCCAAA TTATGCTGGT
CCCCTTATGT TGGGATTGCT TTTGGCTGTT ATTGGTGGAC TTGTGTATCT TCGAAGAAGT
AATATGGAAT TTCTCTTTAA TAAAACTGGA TGGGCTTTTG CAGCTTTGTG TTTTGTGCTT
GCTATGACAT CTGGTCAAAT GTGGAACCAT ATAAGAGGAC CACCATATGC CCATAAGAAT
CCCCACACGG GACATGTGAA TTATATCCAT GGAAGCAGTC AAGCCCAGTT TGTAGCTGAA
ACACACATTG TTCTTCTGTT TAATGGTGGA GTTACCTTAG GAATGGTGCT TTTATGTGAA
GCTGCTACCT CTGACATGGA TATTGGAAAG CGAAAGATAA TGTGTGTGGC TGGTATTGGA
CTTGTTGTAT TATTCTTCAG TTGGATGCTC TCTATTTTTA GATCTAAATA TCATGGCTAC
CCATACAGCT TTCTGATGAG TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_115497.4
CDS63..1166
Misc Feature(1)51..53(+)
Misc Feature(2)339..671(+)
Misc Feature(3)417..428(+)
Misc Feature(4)651..1121(+)
Exon (1)1..260
Gene:MAGT1
Gene Synonym:
Exon (2)261..430
Gene:MAGT1
Gene Synonym:
Exon (3)431..548
Gene:MAGT1
Gene Synonym:
Exon (4)549..689
Gene:MAGT1
Gene Synonym:
Exon (5)690..830
Gene:MAGT1
Gene Synonym:
Exon (6)831..920
Gene:MAGT1
Gene Synonym:
Exon (7)921..984
Gene:MAGT1
Gene Synonym:
Exon (8)985..1059
Gene:MAGT1
Gene Synonym:
Exon (9)1060..1150
Gene:MAGT1
Gene Synonym:
Exon (10)1151..4022
Gene:MAGT1
Gene Synonym:
Translation
Position Chain Variation Link
107 107 c, t dbSNP:398124426
115 115 a, c, t dbSNP:113894525
471 471 c, t dbSNP:387906724
809 809 a, t dbSNP:61732679
994 994 g, t dbSNP:137852222
1624 1624 c, t dbSNP:112880685
2713 2713 g, t dbSNP:113462031
2913 2913 a, g dbSNP:112407327

Target ORF information:

RefSeq Version NM_032121
Organism Homo sapiens (human)
Definition Homo sapiens magnesium transporter 1 (MAGT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_032121

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGGAAAG GCAAGGGGCC GATTTGCCTG TTCTCACGCC CCACCCTCAG ACCTAGCCGG 
AGCAAAGTTT CACTTATAGA AGGGAGAGGA GCGAACATGG CAGCGCGTTG GCGGTTTTGG
TGTGTCTCTG TGACCATGGT GGTGGCGCTG CTCATCGTTT GCGACGTTCC CTCAGCCTCT
GCCCAAAGAA AGAAGGAGAT GGTGTTATCT GAAAAGGTTA GTCAGCTGAT GGAATGGACT
AACAAAAGAC CTGTAATAAG AATGAATGGA GACAAGTTCC GTCGCCTTGT GAAAGCCCCA
CCGAGAAATT ACTCCGTTAT CGTCATGTTC ACTGCTCTCC AACTGCATAG ACAGTGTGTC
GTTTGCAAGC AAGCTGATGA AGAATTCCAG ATCCTGGCAA ACTCCTGGCG ATACTCCAGT
GCATTCACCA ACAGGATATT TTTTGCCATG GTGGATTTTG ATGAAGGCTC TGATGTATTT
CAGATGCTAA ACATGAATTC AGCTCCAACT TTCATCAACT TTCCTGCAAA AGGGAAACCC
AAACGGGGTG ATACATATGA GTTACAGGTG CGGGGTTTTT CAGCTGAGCA GATTGCCCGG
TGGATCGCCG ACAGAACTGA TGTCAATATT AGAGTGATTA GACCCCCAAA TTATGCTGGT
CCCCTTATGT TGGGATTGCT TTTGGCTGTT ATTGGTGGAC TTGTGTATCT TCGAAGAAGT
AATATGGAAT TTCTCTTTAA TAAAACTGGA TGGGCTTTTG CAGCTTTGTG TTTTGTGCTT
GCTATGACAT CTGGTCAAAT GTGGAACCAT ATAAGAGGAC CACCATATGC CCATAAGAAT
CCCCACACGG GACATGTGAA TTATATCCAT GGAAGCAGTC AAGCCCAGTT TGTAGCTGAA
ACACACATTG TTCTTCTGTT TAATGGTGGA GTTACCTTAG GAATGGTGCT TTTATGTGAA
GCTGCTACCT CTGACATGGA TATTGGAAAG CGAAAGATAA TGTGTGTGGC TGGTATTGGA
CTTGTTGTAT TATTCTTCAG TTGGATGCTC TCTATTTTTA GATCTAAATA TCATGGCTAC
CCATACAGCT TTCTGATGAG TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Loss of MAGT1 abrogates the Mg2+ flux required for T cell signaling and leads to a novel human primary immunodeficiency
Magnes Res 24 (3), S109-S114 (2011)
Li FY, Lenardo MJ and Chaigne-Delalande B.


book

The Mg2+ transporter MagT1 partially rescues cell growth and Mg2+ uptake in cells lacking the channel-kinase TRPM7
FEBS Lett. 585 (14), 2275-2278 (2011)
Deason-Towne F, Perraud AL and Schmitz C.


book

Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebrate embryonic development
Proc. Natl. Acad. Sci. U.S.A. 106 (37), 15750-15755 (2009)
Zhou H and Clapham DE.


book

Oligosaccharyltransferase-subunit mutations in nonsyndromic mental retardation
Am. J. Hum. Genet. 82 (5), 1150-1157 (2008)
Molinari F, Foulquier F, Tarpey PS, Morelle W, Boissel S, Teague J, Edkins S, Futreal PA, Stratton MR, Turner G, Matthijs G, Gecz J, Munnich A and Colleaux L.


book

hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes
Genomics 89 (3), 307-315 (2007)
Lamesch P, Li N, Milstein S, Fan C, Hao T, Szabo G, Hu Z, Venkatesan K, Bethel G, Martin P, Rogers J, Lawlor S, McLaren S, Dricot A, Borick H, Cusick ME, Vandenhaute J, Dunham I, Hill DE and Vidal M.


book

Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits
Biochemistry 44 (16), 5982-5992 (2005)
Shibatani T, David LL, McCormack AL, Frueh K and Skach WR.


book

Identification and characterization of a novel mammalian Mg2+ transporter with channel-like properties
BMC Genomics 6, 48 (2005)
Goytain A and Quamme GA.


book

Oligosaccharyltransferase isoforms that contain different catalytic STT3 subunits have distinct enzymatic properties
Mol. Cell 12 (1), 101-111 (2003)
Kelleher DJ, Karaoglu D, Mandon EC and Gilmore R.


book

Congenital Disorders of N-linked Glycosylation Pathway Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Sparks,S.E. and Krasnewich,D.M.


 
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