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MAF1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAF1
Entrez Gene ID 84232
Full Name MAF1 homolog, negative regulator of RNA polymerase III
General protein information
Preferred Names
repressor of RNA polymerase III transcription MAF1 homolog
Names
repressor of RNA polymerase III transcription MAF1 homolog
homolog of yeast MAF1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

8

8q24.3

Summary This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

610210


mRNA and Protein(s)

mRNA Protein Name
NM_032272 NP_115648 repressor of RNA polymerase III transcription MAF1 homolog


Homo sapiens (human) MAF1 NP_115648.2
Pan troglodytes (chimpanzee) MAF1 XP_001155989.2
Macaca mulatta (Rhesus monkey) MAF1 XP_001089551.1
Canis lupus familiaris (dog) MAF1 XP_005628130.1
Bos taurus (cattle) MAF1 NP_001029596.1
Mus musculus (house mouse) Maf1 NP_001158079.1
Rattus norvegicus (Norway rat) Maf1 NP_001014107.1
Gallus gallus (chicken) MAF1 XP_003643589.2
Danio rerio (zebrafish) maf1 NP_998606.1
Drosophila melanogaster (fruit fly) Maf1 NP_001015167.2
Caenorhabditis elegans C43H8.2 NP_492777.1
Arabidopsis thaliana (thale cress) AT5G13240 NP_196828.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu30790
NM_032272 Homo sapiens MAF1 homolog, negative regulator of RNA polymerase III (MAF1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu30790
Clone ID Related Accession (Same CDS sequence) NM_032272
Accession Version NM_032272.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 771bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product repressor of RNA polymerase III transcription MAF1 homolog
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997000.1, BM721318.1, BE093055.1, BC031273.1, BF339064.1 and BC018714.1. On Jul 14, 2006 this sequence version replaced gi:51243066. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC018714.1, BC031273.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGAAGCTAT TGGAGAACTC GAGCTTTGAA GCCATCAACT CACAGCTGAC TGTGGAGACT 
GGAGATGCCC ACATCATTGG CAGGATTGAG AGCTACTCAT GTAAGATGGC AGGAGACGAC
AAACACATGT TCAAGCAGTT CTGCCAGGAG GGCCAGCCCC ACGTGCTGGA GGCACTTTCT
CCACCCCAGA CTTCAGGACT GAGCCCCAGC AGACTCAGCA AAAGCCAAGG CGGTGAGGAG
GAGGGCCCCC TCAGTGACAA GTGCAGCCGC AAGACCCTCT TCTACCTGAT TGCCACGCTC
AATGAGTCCT TCAGGCCTGA CTATGACTTC AGCACAGCCC GCAGCCATGA GTTCAGCCGG
GAGCCCAGCC TTAGCTGGGT GGTGAATGCA GTCAACTGCA GTCTGTTCTC AGCTGTGCGG
GAGGACTTCA AGGATCTGAA ACCACAGCTG TGGAACGCGG TGGACGAGGA GATCTGCCTG
GCTGAATGTG ACATCTACAG CTATAACCCA GACTTGGACT CAGATCCCTT CGGGGAGGAT
GGTAGCCTCT GGTCCTTCAA CTACTTCTTC TACAACAAGC GGCTCAAGCG AATCGTCTTC
TTTAGCTGCC GTTCCATCAG TGGCTCCACC TACACACCCT CAGAGGCAGG CAACGAGCTG
GACATGGAGC TGGGGGAGGA GGAGGTGGAG GAAGAAAGCA GAAGCGGGGG CAGTGGGGCC
GAGGAGACCA GCACCATGGA GGAGGACAGG GTCCCAGTGA TCTGTATTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_115648.2
CDS502..1272
Misc Feature(1)442..444(+)
Misc Feature(2)571..1107(+)
Misc Feature(3)679..681(+)
Misc Feature(4)691..693(+)
Misc Feature(5)694..696(+)
Misc Feature(6)703..705(+)
Misc Feature(7)709..711(+)
Misc Feature(8)724..726(+)
Misc Feature(9)724..726(+)
Misc Feature(10)1135..1137(+)
Misc Feature(11)1141..1143(+)
Exon (1)1..457
Gene:MAF1
Gene Synonym:
Exon (2)458..584
Gene:MAF1
Gene Synonym:
Exon (3)585..713
Gene:MAF1
Gene Synonym:
Exon (4)714..879
Gene:MAF1
Gene Synonym:
Exon (5)880..1001
Gene:MAF1
Gene Synonym:
Exon (6)1002..1121
Gene:MAF1
Gene Synonym:
Exon (7)1122..1250
Gene:MAF1
Gene Synonym:
Exon (8)1251..1775
Gene:MAF1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_032272
Organism Homo sapiens (human)
Definition Homo sapiens MAF1 homolog, negative regulator of RNA polymerase III (MAF1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_032272

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGAAGCTAT TGGAGAACTC GAGCTTTGAA GCCATCAACT CACAGCTGAC TGTGGAGACT 
GGAGATGCCC ACATCATTGG CAGGATTGAG AGCTACTCAT GTAAGATGGC AGGAGACGAC
AAACACATGT TCAAGCAGTT CTGCCAGGAG GGCCAGCCCC ACGTGCTGGA GGCACTTTCT
CCACCCCAGA CTTCAGGACT GAGCCCCAGC AGACTCAGCA AAAGCCAAGG CGGTGAGGAG
GAGGGCCCCC TCAGTGACAA GTGCAGCCGC AAGACCCTCT TCTACCTGAT TGCCACGCTC
AATGAGTCCT TCAGGCCTGA CTATGACTTC AGCACAGCCC GCAGCCATGA GTTCAGCCGG
GAGCCCAGCC TTAGCTGGGT GGTGAATGCA GTCAACTGCA GTCTGTTCTC AGCTGTGCGG
GAGGACTTCA AGGATCTGAA ACCACAGCTG TGGAACGCGG TGGACGAGGA GATCTGCCTG
GCTGAATGTG ACATCTACAG CTATAACCCA GACTTGGACT CAGATCCCTT CGGGGAGGAT
GGTAGCCTCT GGTCCTTCAA CTACTTCTTC TACAACAAGC GGCTCAAGCG AATCGTCTTC
TTTAGCTGCC GTTCCATCAG TGGCTCCACC TACACACCCT CAGAGGCAGG CAACGAGCTG
GACATGGAGC TGGGGGAGGA GGAGGTGGAG GAAGAAAGCA GAAGCGGGGG CAGTGGGGCC
GAGGAGACCA GCACCATGGA GGAGGACAGG GTCCCAGTGA TCTGTATTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Regulating maf1 expression and its expanding biological functions
PLoS Genet. 11 (1), E1004896 (2015)
Moir RD and Willis IM.


book

Covalent small ubiquitin-like modifier (SUMO) modification of Maf1 protein controls RNA polymerase III-dependent transcription repression
J. Biol. Chem. 288 (26), 19288-19295 (2013)
Rohira AD, Chen CY, Allen JR and Johnson DL.


book

Recovery of RNA polymerase III transcription from the glycerol-repressed state: revisiting the role of protein kinase CK2 in Maf1 phosphoregulation
J. Biol. Chem. 287 (36), 30833-30841 (2012)
Moir RD, Lee J and Willis IM.


book

MAF1: a new target of mTORC1
Biochem. Soc. Trans. 39 (2), 487-491 (2011)
Michels AA.


book

Casein kinase II-mediated phosphorylation of general repressor Maf1 triggers RNA polymerase III activation
Proc. Natl. Acad. Sci. U.S.A. 108 (12), 4926-4931 (2011)
Graczyk D, Debski J, Muszynska G, Bretner M, Lefebvre O and Boguta M.


book

hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes
Genomics 89 (3), 307-315 (2007)
Lamesch P, Li N, Milstein S, Fan C, Hao T, Szabo G, Hu Z, Venkatesan K, Bethel G, Martin P, Rogers J, Lawlor S, McLaren S, Dricot A, Borick H, Cusick ME, Vandenhaute J, Dunham I, Hill DE and Vidal M.


book

Maf1, a new player in the regulation of human RNA polymerase III transcription
PLoS ONE 1, E134 (2006)
Reina JH, Azzouz TN and Hernandez N.


book

Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription
Mol. Cell 10 (6), 1489-1494 (2002)
Upadhya R, Lee J and Willis IM.


book

Maf1p, a negative effector of RNA polymerase III in Saccharomyces cerevisiae
Mol. Cell. Biol. 21 (15), 5031-5040 (2001)
Pluta K, Lefebvre O, Martin NC, Smagowicz WJ, Stanford DR, Ellis SR, Hopper AK, Sentenac A and Boguta M.


book

Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing
EMBO Rep. 1 (3), 287-292 (2000)
Simpson JC, Wellenreuther R, Poustka A, Pepperkok R and Wiemann S.


 
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