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CASR cDNA ORF clone, Homo sapiens (human)

Gene Symbol CASR
Entrez Gene ID 846
Full Name calcium-sensing receptor
Synonyms CAR, EIG8, FHH, FIH, GPRC2A, HHC, HHC1, HYPOC1, NSHPT, PCAR1
General protein information
Preferred Names
extracellular calcium-sensing receptor
Names
extracellular calcium-sensing receptor
parathyroid Ca(2+)-sensing receptor 1
parathyroid cell calcium-sensing receptor
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3q13

Summary The protein encoded by this gene is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601199

Disorder Html: Hypocalciuric hypercalcemia, type I, 145980 (3); Hyperparathyroidism,

mRNA and Protein(s)

mRNA Protein Name
XM_011513237 XP_011511539 extracellular calcium-sensing receptor isoform X1
XM_006713789 XP_006713852 extracellular calcium-sensing receptor isoform X1
XM_011513238 XP_011511540 extracellular calcium-sensing receptor isoform X1
XM_005247836 XP_005247893 extracellular calcium-sensing receptor isoform X1
XM_005247837 XP_005247894 extracellular calcium-sensing receptor isoform X2
XM_011513239 XP_011511541 extracellular calcium-sensing receptor isoform X3
NM_001178065 NP_001171536 extracellular calcium-sensing receptor isoform 1 precursor
NM_000388 NP_000379 extracellular calcium-sensing receptor isoform 2 precursor



Homo sapiens (human) CASR NP_001171536.1
Pan troglodytes (chimpanzee) CASR XP_516689.2
Macaca mulatta (Rhesus monkey) CASR XP_001112009.2
Canis lupus familiaris (dog) CASR NP_001074978.1
Bos taurus (cattle) CASR NP_776427.1
Mus musculus (house mouse) Casr NP_038831.2
Rattus norvegicus (Norway rat) Casr NP_058692.1
Gallus gallus (chicken) CASR XP_416491.3
Danio rerio (zebrafish) LOC560607 XP_689097.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100496505 XP_004919842.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CASR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CASR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu38198 XM_011513237 PREDICTED: Homo sapiens calcium-sensing receptor (CASR), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $559.30
$799.00
OHu38198 XM_006713789 PREDICTED: Homo sapiens calcium-sensing receptor (CASR), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $559.30
$799.00
OHu38198 XM_011513238 PREDICTED: Homo sapiens calcium-sensing receptor (CASR), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $559.30
$799.00
OHu38198 XM_005247836 PREDICTED: Homo sapiens calcium-sensing receptor (CASR), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $559.30
$799.00
OHu38199 XM_005247837 PREDICTED: Homo sapiens calcium-sensing receptor (CASR), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu56661 XM_011513239 PREDICTED: Homo sapiens calcium-sensing receptor (CASR), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $475.30
$679.00
OHu15961 NM_001178065 Homo sapiens calcium-sensing receptor (CASR), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu27046
NM_000388 Homo sapiens calcium-sensing receptor (CASR), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu38198
Clone ID Related Accession (Same CDS sequence) XM_005247836 , XM_006713789 , XM_011513237 , XM_011513238
Accession Version XM_011513237.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product extracellular calcium-sensing receptor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511539.1
CDS2478..5714
Misc Feature(1)2535..4067(+)
Misc Feature(2)2673..3725(+)
Misc Feature(3)2682..3962(+)
Misc Feature(4)2790..3203(+)
Misc Feature(5)4089..4250(+)
Misc Feature(6)4341..5057(+)
Misc Feature(7)4521..5039(+)
Translation

Target ORF information:

RefSeq Version XM_011513237
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium-sensing receptor (CASR), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513237

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38198
Clone ID Related Accession (Same CDS sequence) XM_005247836 , XM_006713789 , XM_011513237 , XM_011513238
Accession Version XM_006713789.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product extracellular calcium-sensing receptor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578807817. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713852.1
CDS458..3694
Misc Feature(1)515..2047(+)
Misc Feature(2)653..1705(+)
Misc Feature(3)662..1942(+)
Misc Feature(4)770..1183(+)
Misc Feature(5)2069..2230(+)
Misc Feature(6)2321..3037(+)
Misc Feature(7)2501..3019(+)
Translation

Target ORF information:

RefSeq Version XM_006713789
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium-sensing receptor (CASR), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006713789

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38198
Clone ID Related Accession (Same CDS sequence) XM_005247836 , XM_006713789 , XM_011513237 , XM_011513238
Accession Version XM_011513238.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product extracellular calcium-sensing receptor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511540.1
CDS2409..5645
Misc Feature(1)2466..3998(+)
Misc Feature(2)2604..3656(+)
Misc Feature(3)2613..3893(+)
Misc Feature(4)2721..3134(+)
Misc Feature(5)4020..4181(+)
Misc Feature(6)4272..4988(+)
Misc Feature(7)4452..4970(+)
Translation

Target ORF information:

RefSeq Version XM_011513238
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium-sensing receptor (CASR), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513238

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38198
Clone ID Related Accession (Same CDS sequence) XM_005247836 , XM_006713789 , XM_011513237 , XM_011513238
Accession Version XM_005247836.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product extracellular calcium-sensing receptor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530375404. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005247893.1
CDS607..3843
Misc Feature(1)664..2196(+)
Misc Feature(2)802..1854(+)
Misc Feature(3)811..2091(+)
Misc Feature(4)919..1332(+)
Misc Feature(5)2218..2379(+)
Misc Feature(6)2470..3186(+)
Misc Feature(7)2650..3168(+)
Translation

Target ORF information:

RefSeq Version XM_005247836
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium-sensing receptor (CASR), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005247836

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38199
Clone ID Related Accession (Same CDS sequence) XM_005247837
Accession Version XM_005247837.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product extracellular calcium-sensing receptor isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530375406. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGTATCAGG TCAGTTATGC CTCCTCCAGC AGACTCCTCA GCAACAAGAA TCAATTCAAG 
TCTTTCCTCC GAACCATCCC CAATGATGAG CACCAGGCCA CTGCCATGGC AGACATCATC
GAGTATTTCC GCTGGAACTG GGTGGGCACA ATTGCAGCTG ATGACGACTA TGGGCGGCCG
GGGATTGAGA AATTCCGAGA GGAAGCTGAG GAAAGGGATA TCTGCATCGA CTTCAGTGAA
CTCATCTCCC AGTACTCTGA TGAGGAAGAG ATCCAGCATG TGGTAGAGGT GATTCAAAAT
TCCACGGCCA AAGTCATCGT GGTTTTCTCC AGTGGCCCAG ATCTTGAGCC CCTCATCAAG
GAGATTGTCC GGCGCAATAT CACGGGCAAG ATCTGGCTGG CCAGCGAGGC CTGGGCCAGC
TCCTCCCTGA TCGCCATGCC TCAGTACTTC CACGTGGTTG GCGGCACCAT TGGATTCGCT
CTGAAGGCTG GGCAGATCCC AGGCTTCCGG GAATTCCTGA AGAAGGTCCA TCCCAGGAAG
TCTGTCCACA ATGGTTTTGC CAAGGAGTTT TGGGAAGAAA CATTTAACTG CCACCTCCAA
GAAGGTGCAA AAGGACCTTT ACCTGTGGAC ACCTTTCTGA GAGGTCACGA AGAAAGTGGC
GACAGGTTTA GCAACAGCTC GACAGCCTTC CGACCCCTCT GTACAGGGGA TGAGAACATC
AGCAGTGTCG AGACCCCTTA CATAGATTAC ACGCATTTAC GGATATCCTA CAATGTGTAC
TTAGCAGTCT ACTCCATTGC CCACGCCTTG CAAGATATAT ATACCTGCTT ACCTGGGAGA
GGGCTCTTCA CCAATGGCTC CTGTGCAGAC ATCAAGAAAG TTGAGGCGTG GCAGGTCCTG
AAGCACCTAC GGCATCTAAA CTTTACAAAC AATATGGGGG AGCAGGTGAC CTTTGATGAG
TGTGGTGACC TGGTGGGGAA CTATTCCATC ATCAACTGGC ACCTCTCCCC AGAGGATGGC
TCCATCGTGT TTAAGGAAGT CGGGTATTAC AACGTCTATG CCAAGAAGGG AGAAAGACTC
TTCATCAACG AGGAGAAAAT CCTGTGGAGT GGGTTCTCCA GGGAGGTGCC CTTCTCCAAC
TGCAGCCGAG ACTGCCTGGC AGGGACCAGG AAAGGGATCA TTGAGGGGGA GCCCACCTGC
TGCTTTGAGT GTGTGGAGTG TCCTGATGGG GAGTATAGTG ATGAGACAGA TGCCAGTGCC
TGTAACAAGT GCCCAGATGA CTTCTGGTCC AATGAGAACC ACACCTCCTG CATTGCCAAG
GAGATCGAGT TTCTGTCGTG GACGGAGCCC TTTGGGATCG CACTCACCCT CTTTGCCGTG
CTGGGCATTT TCCTGACAGC CTTTGTGCTG GGTGTGTTTA TCAAGTTCCG CAACACACCC
ATTGTCAAGG CCACCAACCG AGAGCTCTCC TACCTCCTCC TCTTCTCCCT GCTCTGCTGC
TTCTCCAGCT CCCTGTTCTT CATCGGGGAG CCCCAGGACT GGACGTGCCG CCTGCGCCAG
CCGGCCTTTG GCATCAGCTT CGTGCTCTGC ATCTCATGCA TCCTGGTGAA AACCAACCGT
GTCCTCCTGG TGTTTGAGGC CAAGATCCCC ACCAGCTTCC ACCGCAAGTG GTGGGGGCTC
AACCTGCAGT TCCTGCTGGT TTTCCTCTGC ACCTTCATGC AGATTGTCAT CTGTGTGATC
TGGCTCTACA CCGCGCCCCC GTCAAGCTAC CGCAACCAGG AGCTGGAGGA TGAGATCATC
TTCATCACGT GCCACGAGGG CTCCCTCATG GCCCTGGGCT TCCTGATCGG CTACACCTGC
CTGCTGGCTG CCATCTGCTT CTTCTTTGCC TTCAAGTCCC GGAAGCTGCC GGAGAACTTC
AATGAAGCCA AGTTCATCAC CTTCAGCATG CTCATCTTCT TCATCGTCTG GATCTCCTTC
ATTCCAGCCT ATGCCAGCAC CTATGGCAAG TTTGTCTCTG CCGTAGAGGT GATTGCCATC
CTGGCAGCCA GCTTTGGCTT GCTGGCGTGC ATCTTCTTCA ACAAGATCTA CATCATTCTC
TTCAAGCCAT CCCGCAACAC CATCGAGGAG GTGCGTTGCA GCACCGCAGC TCACGCTTTC
AAGGTGGCTG CCCGGGCCAC GCTGCGCCGC AGCAACGTCT CCCGCAAGCG GTCCAGCAGC
CTTGGAGGCT CCACGGGATC CACCCCCTCC TCCTCCATCA GCAGCAAGAG CAACAGCGAA
GACCCATTCC CACAGCCCGA GAGGCAGAAG CAGCAGCAGC CGCTGGCCCT AACCCAGCAA
GAGCAGCAGC AGCAGCCCCT GACCCTCCCA CAGCAGCAAC GATCTCAGCA GCAGCCCAGA
TGCAAGCAGA AGGTCATCTT TGGCAGCGGC ACGGTCACCT TCTCACTGAG CTTTGATGAG
CCTCAGAAGA ACGCCATGGC CCACAGGAAT TCTACGCACC AGAACTCCCT GGAGGCCCAG
AAAAGCAGCG ATACGCTGAC CCGACACGAG CCATTACTCC CGCTGCAGTG CGGGGAAACG
GACTTAGATC TGACCGTCCA GGAAACAGGT CTGCAAGGAC CTGTGGGTGG AGACCAGCGG
CCAGAGGTGG AGGACCCTGA AGAGTTGTCC CCAGCACTTG TAGTGTCCAG TTCACAGAGC
TTTGTCATCA GTGGTGGAGG CAGCACTGTT ACAGAAAACG TAGTGAATTC ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005247894.1
CDS86..2839
Misc Feature(1)92..1192(+)
Misc Feature(2)92..1087(+)
Misc Feature(3)1214..1375(+)
Misc Feature(4)1466..2182(+)
Misc Feature(5)1646..2164(+)
Translation

Target ORF information:

RefSeq Version XM_005247837
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium-sensing receptor (CASR), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005247837

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGTATCAGG TCAGTTATGC CTCCTCCAGC AGACTCCTCA GCAACAAGAA TCAATTCAAG 
TCTTTCCTCC GAACCATCCC CAATGATGAG CACCAGGCCA CTGCCATGGC AGACATCATC
GAGTATTTCC GCTGGAACTG GGTGGGCACA ATTGCAGCTG ATGACGACTA TGGGCGGCCG
GGGATTGAGA AATTCCGAGA GGAAGCTGAG GAAAGGGATA TCTGCATCGA CTTCAGTGAA
CTCATCTCCC AGTACTCTGA TGAGGAAGAG ATCCAGCATG TGGTAGAGGT GATTCAAAAT
TCCACGGCCA AAGTCATCGT GGTTTTCTCC AGTGGCCCAG ATCTTGAGCC CCTCATCAAG
GAGATTGTCC GGCGCAATAT CACGGGCAAG ATCTGGCTGG CCAGCGAGGC CTGGGCCAGC
TCCTCCCTGA TCGCCATGCC TCAGTACTTC CACGTGGTTG GCGGCACCAT TGGATTCGCT
CTGAAGGCTG GGCAGATCCC AGGCTTCCGG GAATTCCTGA AGAAGGTCCA TCCCAGGAAG
TCTGTCCACA ATGGTTTTGC CAAGGAGTTT TGGGAAGAAA CATTTAACTG CCACCTCCAA
GAAGGTGCAA AAGGACCTTT ACCTGTGGAC ACCTTTCTGA GAGGTCACGA AGAAAGTGGC
GACAGGTTTA GCAACAGCTC GACAGCCTTC CGACCCCTCT GTACAGGGGA TGAGAACATC
AGCAGTGTCG AGACCCCTTA CATAGATTAC ACGCATTTAC GGATATCCTA CAATGTGTAC
TTAGCAGTCT ACTCCATTGC CCACGCCTTG CAAGATATAT ATACCTGCTT ACCTGGGAGA
GGGCTCTTCA CCAATGGCTC CTGTGCAGAC ATCAAGAAAG TTGAGGCGTG GCAGGTCCTG
AAGCACCTAC GGCATCTAAA CTTTACAAAC AATATGGGGG AGCAGGTGAC CTTTGATGAG
TGTGGTGACC TGGTGGGGAA CTATTCCATC ATCAACTGGC ACCTCTCCCC AGAGGATGGC
TCCATCGTGT TTAAGGAAGT CGGGTATTAC AACGTCTATG CCAAGAAGGG AGAAAGACTC
TTCATCAACG AGGAGAAAAT CCTGTGGAGT GGGTTCTCCA GGGAGGTGCC CTTCTCCAAC
TGCAGCCGAG ACTGCCTGGC AGGGACCAGG AAAGGGATCA TTGAGGGGGA GCCCACCTGC
TGCTTTGAGT GTGTGGAGTG TCCTGATGGG GAGTATAGTG ATGAGACAGA TGCCAGTGCC
TGTAACAAGT GCCCAGATGA CTTCTGGTCC AATGAGAACC ACACCTCCTG CATTGCCAAG
GAGATCGAGT TTCTGTCGTG GACGGAGCCC TTTGGGATCG CACTCACCCT CTTTGCCGTG
CTGGGCATTT TCCTGACAGC CTTTGTGCTG GGTGTGTTTA TCAAGTTCCG CAACACACCC
ATTGTCAAGG CCACCAACCG AGAGCTCTCC TACCTCCTCC TCTTCTCCCT GCTCTGCTGC
TTCTCCAGCT CCCTGTTCTT CATCGGGGAG CCCCAGGACT GGACGTGCCG CCTGCGCCAG
CCGGCCTTTG GCATCAGCTT CGTGCTCTGC ATCTCATGCA TCCTGGTGAA AACCAACCGT
GTCCTCCTGG TGTTTGAGGC CAAGATCCCC ACCAGCTTCC ACCGCAAGTG GTGGGGGCTC
AACCTGCAGT TCCTGCTGGT TTTCCTCTGC ACCTTCATGC AGATTGTCAT CTGTGTGATC
TGGCTCTACA CCGCGCCCCC GTCAAGCTAC CGCAACCAGG AGCTGGAGGA TGAGATCATC
TTCATCACGT GCCACGAGGG CTCCCTCATG GCCCTGGGCT TCCTGATCGG CTACACCTGC
CTGCTGGCTG CCATCTGCTT CTTCTTTGCC TTCAAGTCCC GGAAGCTGCC GGAGAACTTC
AATGAAGCCA AGTTCATCAC CTTCAGCATG CTCATCTTCT TCATCGTCTG GATCTCCTTC
ATTCCAGCCT ATGCCAGCAC CTATGGCAAG TTTGTCTCTG CCGTAGAGGT GATTGCCATC
CTGGCAGCCA GCTTTGGCTT GCTGGCGTGC ATCTTCTTCA ACAAGATCTA CATCATTCTC
TTCAAGCCAT CCCGCAACAC CATCGAGGAG GTGCGTTGCA GCACCGCAGC TCACGCTTTC
AAGGTGGCTG CCCGGGCCAC GCTGCGCCGC AGCAACGTCT CCCGCAAGCG GTCCAGCAGC
CTTGGAGGCT CCACGGGATC CACCCCCTCC TCCTCCATCA GCAGCAAGAG CAACAGCGAA
GACCCATTCC CACAGCCCGA GAGGCAGAAG CAGCAGCAGC CGCTGGCCCT AACCCAGCAA
GAGCAGCAGC AGCAGCCCCT GACCCTCCCA CAGCAGCAAC GATCTCAGCA GCAGCCCAGA
TGCAAGCAGA AGGTCATCTT TGGCAGCGGC ACGGTCACCT TCTCACTGAG CTTTGATGAG
CCTCAGAAGA ACGCCATGGC CCACAGGAAT TCTACGCACC AGAACTCCCT GGAGGCCCAG
AAAAGCAGCG ATACGCTGAC CCGACACGAG CCATTACTCC CGCTGCAGTG CGGGGAAACG
GACTTAGATC TGACCGTCCA GGAAACAGGT CTGCAAGGAC CTGTGGGTGG AGACCAGCGG
CCAGAGGTGG AGGACCCTGA AGAGTTGTCC CCAGCACTTG TAGTGTCCAG TTCACAGAGC
TTTGTCATCA GTGGTGGAGG CAGCACTGTT ACAGAAAACG TAGTGAATTC ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56661
Clone ID Related Accession (Same CDS sequence) XM_011513239
Accession Version XM_011513239.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2649bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product extracellular calcium-sensing receptor isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCAGACA TCATCGAGTA TTTCCGCTGG AACTGGGTGG GCACAATTGC AGCTGATGAC 
GACTATGGGC GGCCGGGGAT TGAGAAATTC CGAGAGGAAG CTGAGGAAAG GGATATCTGC
ATCGACTTCA GTGAACTCAT CTCCCAGTAC TCTGATGAGG AAGAGATCCA GCATGTGGTA
GAGGTGATTC AAAATTCCAC GGCCAAAGTC ATCGTGGTTT TCTCCAGTGG CCCAGATCTT
GAGCCCCTCA TCAAGGAGAT TGTCCGGCGC AATATCACGG GCAAGATCTG GCTGGCCAGC
GAGGCCTGGG CCAGCTCCTC CCTGATCGCC ATGCCTCAGT ACTTCCACGT GGTTGGCGGC
ACCATTGGAT TCGCTCTGAA GGCTGGGCAG ATCCCAGGCT TCCGGGAATT CCTGAAGAAG
GTCCATCCCA GGAAGTCTGT CCACAATGGT TTTGCCAAGG AGTTTTGGGA AGAAACATTT
AACTGCCACC TCCAAGAAGG TGCAAAAGGA CCTTTACCTG TGGACACCTT TCTGAGAGGT
CACGAAGAAA GTGGCGACAG GTTTAGCAAC AGCTCGACAG CCTTCCGACC CCTCTGTACA
GGGGATGAGA ACATCAGCAG TGTCGAGACC CCTTACATAG ATTACACGCA TTTACGGATA
TCCTACAATG TGTACTTAGC AGTCTACTCC ATTGCCCACG CCTTGCAAGA TATATATACC
TGCTTACCTG GGAGAGGGCT CTTCACCAAT GGCTCCTGTG CAGACATCAA GAAAGTTGAG
GCGTGGCAGG TCCTGAAGCA CCTACGGCAT CTAAACTTTA CAAACAATAT GGGGGAGCAG
GTGACCTTTG ATGAGTGTGG TGACCTGGTG GGGAACTATT CCATCATCAA CTGGCACCTC
TCCCCAGAGG ATGGCTCCAT CGTGTTTAAG GAAGTCGGGT ATTACAACGT CTATGCCAAG
AAGGGAGAAA GACTCTTCAT CAACGAGGAG AAAATCCTGT GGAGTGGGTT CTCCAGGGAG
GTGCCCTTCT CCAACTGCAG CCGAGACTGC CTGGCAGGGA CCAGGAAAGG GATCATTGAG
GGGGAGCCCA CCTGCTGCTT TGAGTGTGTG GAGTGTCCTG ATGGGGAGTA TAGTGATGAG
ACAGATGCCA GTGCCTGTAA CAAGTGCCCA GATGACTTCT GGTCCAATGA GAACCACACC
TCCTGCATTG CCAAGGAGAT CGAGTTTCTG TCGTGGACGG AGCCCTTTGG GATCGCACTC
ACCCTCTTTG CCGTGCTGGG CATTTTCCTG ACAGCCTTTG TGCTGGGTGT GTTTATCAAG
TTCCGCAACA CACCCATTGT CAAGGCCACC AACCGAGAGC TCTCCTACCT CCTCCTCTTC
TCCCTGCTCT GCTGCTTCTC CAGCTCCCTG TTCTTCATCG GGGAGCCCCA GGACTGGACG
TGCCGCCTGC GCCAGCCGGC CTTTGGCATC AGCTTCGTGC TCTGCATCTC ATGCATCCTG
GTGAAAACCA ACCGTGTCCT CCTGGTGTTT GAGGCCAAGA TCCCCACCAG CTTCCACCGC
AAGTGGTGGG GGCTCAACCT GCAGTTCCTG CTGGTTTTCC TCTGCACCTT CATGCAGATT
GTCATCTGTG TGATCTGGCT CTACACCGCG CCCCCGTCAA GCTACCGCAA CCAGGAGCTG
GAGGATGAGA TCATCTTCAT CACGTGCCAC GAGGGCTCCC TCATGGCCCT GGGCTTCCTG
ATCGGCTACA CCTGCCTGCT GGCTGCCATC TGCTTCTTCT TTGCCTTCAA GTCCCGGAAG
CTGCCGGAGA ACTTCAATGA AGCCAAGTTC ATCACCTTCA GCATGCTCAT CTTCTTCATC
GTCTGGATCT CCTTCATTCC AGCCTATGCC AGCACCTATG GCAAGTTTGT CTCTGCCGTA
GAGGTGATTG CCATCCTGGC AGCCAGCTTT GGCTTGCTGG CGTGCATCTT CTTCAACAAG
ATCTACATCA TTCTCTTCAA GCCATCCCGC AACACCATCG AGGAGGTGCG TTGCAGCACC
GCAGCTCACG CTTTCAAGGT GGCTGCCCGG GCCACGCTGC GCCGCAGCAA CGTCTCCCGC
AAGCGGTCCA GCAGCCTTGG AGGCTCCACG GGATCCACCC CCTCCTCCTC CATCAGCAGC
AAGAGCAACA GCGAAGACCC ATTCCCACAG CCCGAGAGGC AGAAGCAGCA GCAGCCGCTG
GCCCTAACCC AGCAAGAGCA GCAGCAGCAG CCCCTGACCC TCCCACAGCA GCAACGATCT
CAGCAGCAGC CCAGATGCAA GCAGAAGGTC ATCTTTGGCA GCGGCACGGT CACCTTCTCA
CTGAGCTTTG ATGAGCCTCA GAAGAACGCC ATGGCCCACA GGAATTCTAC GCACCAGAAC
TCCCTGGAGG CCCAGAAAAG CAGCGATACG CTGACCCGAC ACGAGCCATT ACTCCCGCTG
CAGTGCGGGG AAACGGACTT AGATCTGACC GTCCAGGAAA CAGGTCTGCA AGGACCTGTG
GGTGGAGACC AGCGGCCAGA GGTGGAGGAC CCTGAAGAGT TGTCCCCAGC ACTTGTAGTG
TCCAGTTCAC AGAGCTTTGT CATCAGTGGT GGAGGCAGCA CTGTTACAGA AAACGTAGTG
AATTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511541.1
CDS184..2832
Misc Feature(1)184..1185(+)
Misc Feature(2)184..1080(+)
Misc Feature(3)1207..1368(+)
Misc Feature(4)1459..2175(+)
Misc Feature(5)1639..2157(+)
Translation

Target ORF information:

RefSeq Version XM_011513239
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium-sensing receptor (CASR), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513239

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCAGACA TCATCGAGTA TTTCCGCTGG AACTGGGTGG GCACAATTGC AGCTGATGAC 
GACTATGGGC GGCCGGGGAT TGAGAAATTC CGAGAGGAAG CTGAGGAAAG GGATATCTGC
ATCGACTTCA GTGAACTCAT CTCCCAGTAC TCTGATGAGG AAGAGATCCA GCATGTGGTA
GAGGTGATTC AAAATTCCAC GGCCAAAGTC ATCGTGGTTT TCTCCAGTGG CCCAGATCTT
GAGCCCCTCA TCAAGGAGAT TGTCCGGCGC AATATCACGG GCAAGATCTG GCTGGCCAGC
GAGGCCTGGG CCAGCTCCTC CCTGATCGCC ATGCCTCAGT ACTTCCACGT GGTTGGCGGC
ACCATTGGAT TCGCTCTGAA GGCTGGGCAG ATCCCAGGCT TCCGGGAATT CCTGAAGAAG
GTCCATCCCA GGAAGTCTGT CCACAATGGT TTTGCCAAGG AGTTTTGGGA AGAAACATTT
AACTGCCACC TCCAAGAAGG TGCAAAAGGA CCTTTACCTG TGGACACCTT TCTGAGAGGT
CACGAAGAAA GTGGCGACAG GTTTAGCAAC AGCTCGACAG CCTTCCGACC CCTCTGTACA
GGGGATGAGA ACATCAGCAG TGTCGAGACC CCTTACATAG ATTACACGCA TTTACGGATA
TCCTACAATG TGTACTTAGC AGTCTACTCC ATTGCCCACG CCTTGCAAGA TATATATACC
TGCTTACCTG GGAGAGGGCT CTTCACCAAT GGCTCCTGTG CAGACATCAA GAAAGTTGAG
GCGTGGCAGG TCCTGAAGCA CCTACGGCAT CTAAACTTTA CAAACAATAT GGGGGAGCAG
GTGACCTTTG ATGAGTGTGG TGACCTGGTG GGGAACTATT CCATCATCAA CTGGCACCTC
TCCCCAGAGG ATGGCTCCAT CGTGTTTAAG GAAGTCGGGT ATTACAACGT CTATGCCAAG
AAGGGAGAAA GACTCTTCAT CAACGAGGAG AAAATCCTGT GGAGTGGGTT CTCCAGGGAG
GTGCCCTTCT CCAACTGCAG CCGAGACTGC CTGGCAGGGA CCAGGAAAGG GATCATTGAG
GGGGAGCCCA CCTGCTGCTT TGAGTGTGTG GAGTGTCCTG ATGGGGAGTA TAGTGATGAG
ACAGATGCCA GTGCCTGTAA CAAGTGCCCA GATGACTTCT GGTCCAATGA GAACCACACC
TCCTGCATTG CCAAGGAGAT CGAGTTTCTG TCGTGGACGG AGCCCTTTGG GATCGCACTC
ACCCTCTTTG CCGTGCTGGG CATTTTCCTG ACAGCCTTTG TGCTGGGTGT GTTTATCAAG
TTCCGCAACA CACCCATTGT CAAGGCCACC AACCGAGAGC TCTCCTACCT CCTCCTCTTC
TCCCTGCTCT GCTGCTTCTC CAGCTCCCTG TTCTTCATCG GGGAGCCCCA GGACTGGACG
TGCCGCCTGC GCCAGCCGGC CTTTGGCATC AGCTTCGTGC TCTGCATCTC ATGCATCCTG
GTGAAAACCA ACCGTGTCCT CCTGGTGTTT GAGGCCAAGA TCCCCACCAG CTTCCACCGC
AAGTGGTGGG GGCTCAACCT GCAGTTCCTG CTGGTTTTCC TCTGCACCTT CATGCAGATT
GTCATCTGTG TGATCTGGCT CTACACCGCG CCCCCGTCAA GCTACCGCAA CCAGGAGCTG
GAGGATGAGA TCATCTTCAT CACGTGCCAC GAGGGCTCCC TCATGGCCCT GGGCTTCCTG
ATCGGCTACA CCTGCCTGCT GGCTGCCATC TGCTTCTTCT TTGCCTTCAA GTCCCGGAAG
CTGCCGGAGA ACTTCAATGA AGCCAAGTTC ATCACCTTCA GCATGCTCAT CTTCTTCATC
GTCTGGATCT CCTTCATTCC AGCCTATGCC AGCACCTATG GCAAGTTTGT CTCTGCCGTA
GAGGTGATTG CCATCCTGGC AGCCAGCTTT GGCTTGCTGG CGTGCATCTT CTTCAACAAG
ATCTACATCA TTCTCTTCAA GCCATCCCGC AACACCATCG AGGAGGTGCG TTGCAGCACC
GCAGCTCACG CTTTCAAGGT GGCTGCCCGG GCCACGCTGC GCCGCAGCAA CGTCTCCCGC
AAGCGGTCCA GCAGCCTTGG AGGCTCCACG GGATCCACCC CCTCCTCCTC CATCAGCAGC
AAGAGCAACA GCGAAGACCC ATTCCCACAG CCCGAGAGGC AGAAGCAGCA GCAGCCGCTG
GCCCTAACCC AGCAAGAGCA GCAGCAGCAG CCCCTGACCC TCCCACAGCA GCAACGATCT
CAGCAGCAGC CCAGATGCAA GCAGAAGGTC ATCTTTGGCA GCGGCACGGT CACCTTCTCA
CTGAGCTTTG ATGAGCCTCA GAAGAACGCC ATGGCCCACA GGAATTCTAC GCACCAGAAC
TCCCTGGAGG CCCAGAAAAG CAGCGATACG CTGACCCGAC ACGAGCCATT ACTCCCGCTG
CAGTGCGGGG AAACGGACTT AGATCTGACC GTCCAGGAAA CAGGTCTGCA AGGACCTGTG
GGTGGAGACC AGCGGCCAGA GGTGGAGGAC CCTGAAGAGT TGTCCCCAGC ACTTGTAGTG
TCCAGTTCAC AGAGCTTTGT CATCAGTGGT GGAGGCAGCA CTGTTACAGA AAACGTAGTG
AATTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15961
Clone ID Related Accession (Same CDS sequence) NM_001178065
Accession Version NM_001178065.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3267bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product extracellular calcium-sensing receptor isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U20760.1, BC104999.1 and AC068754.16. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U20760.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1970526, SAMEA2145774 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGCC ACTCACCTTT
GTGCTGTCTG TCCTCCAGGT GCCCTTCTCC AACTGCAGCC GAGACTGCCT GGCAGGGACC
AGGAAAGGGA TCATTGAGGG GGAGCCCACC TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT
GGGGAGTATA GTGATGAGAC AGATGCCAGT GCCTGTAACA AGTGCCCAGA TGACTTCTGG
TCCAATGAGA ACCACACCTC CTGCATTGCC AAGGAGATCG AGTTTCTGTC GTGGACGGAG
CCCTTTGGGA TCGCACTCAC CCTCTTTGCC GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG
CTGGGTGTGT TTATCAAGTT CCGCAACACA CCCATTGTCA AGGCCACCAA CCGAGAGCTC
TCCTACCTCC TCCTCTTCTC CCTGCTCTGC TGCTTCTCCA GCTCCCTGTT CTTCATCGGG
GAGCCCCAGG ACTGGACGTG CCGCCTGCGC CAGCCGGCCT TTGGCATCAG CTTCGTGCTC
TGCATCTCAT GCATCCTGGT GAAAACCAAC CGTGTCCTCC TGGTGTTTGA GGCCAAGATC
CCCACCAGCT TCCACCGCAA GTGGTGGGGG CTCAACCTGC AGTTCCTGCT GGTTTTCCTC
TGCACCTTCA TGCAGATTGT CATCTGTGTG ATCTGGCTCT ACACCGCGCC CCCCTCAAGC
TACCGCAACC AGGAGCTGGA GGATGAGATC ATCTTCATCA CGTGCCACGA GGGCTCCCTC
ATGGCCCTGG GCTTCCTGAT CGGCTACACC TGCCTGCTGG CTGCCATCTG CTTCTTCTTT
GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC TTCAATGAAG CCAAGTTCAT CACCTTCAGC
ATGCTCATCT TCTTCATCGT CTGGATCTCC TTCATTCCAG CCTATGCCAG CACCTATGGC
AAGTTTGTCT CTGCCGTAGA GGTGATTGCC ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG
TGCATCTTCT TCAACAAGAT CTACATCATT CTCTTCAAGC CATCCCGCAA CACCATCGAG
GAGGTGCGTT GCAGCACCGC AGCTCACGCT TTCAAGGTGG CTGCCCGGGC CACGCTGCGC
CGCAGCAACG TCTCCCGCAA GCGGTCCAGC AGCCTTGGAG GCTCCACGGG ATCCACCCCC
TCCTCCTCCA TCAGCAGCAA GAGCAACAGC GAAGACCCAT TCCCACAGCC CGAGAGGCAG
AAGCAGCAGC AGCCGCTGGC CCTAACCCAG CAAGAGCAGC AGCAGCAGCC CCTGACCCTC
CCACAGCAGC AACGATCTCA GCAGCAGCCC AGATGCAAGC AGAAGGTCAT CTTTGGCAGC
GGCACGGTCA CCTTCTCACT GAGCTTTGAT GAGCCTCAGA AGAACGCCAT GGCCCACAGG
AATTCTACGC ACCAGAACTC CCTGGAGGCC CAGAAAAGCA GCGATACGCT GACCCGACAC
CAGCCATTAC TCCCGCTGCA GTGCGGGGAA ACGGACTTAG ATCTGACCGT CCAGGAAACA
GGTCTGCAAG GACCTGTGGG TGGAGACCAG CGGCCAGAGG TGGAGGACCC TGAAGAGTTG
TCCCCAGCAC TTGTAGTGTC CAGTTCACAG AGCTTTGTCA TCAGTGGTGG AGGCAGCACT
GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001171536.1
CDS439..3705
Misc Feature(1)400..402(+)
Misc Feature(2)496..2028(+)
Misc Feature(3)634..1686(+)
Misc Feature(4)643..1923(+)
Misc Feature(5)751..1164(+)
Misc Feature(6)2080..2241(+)
Misc Feature(7)2305..2373(+)
Misc Feature(8)2332..3048(+)
Misc Feature(9)2416..2478(+)
Misc Feature(10)2512..3030(+)
Misc Feature(11)2512..2568(+)
Misc Feature(12)2641..2703(+)
Misc Feature(13)2776..2844(+)
Misc Feature(14)2884..2952(+)
Misc Feature(15)2977..3054(+)
Misc Feature(16)3106..3168(+)
Exon (1)1..196
Gene:CASR
Gene Synonym:
Exon (2)197..623
Gene:CASR
Gene Synonym:
Exon (3)624..930
Gene:CASR
Gene Synonym:
Exon (4)931..1815
Gene:CASR
Gene Synonym:
Exon (5)1816..2046
Gene:CASR
Gene Synonym:
Exon (6)2047..2200
Gene:CASR
Gene Synonym:
Exon (7)2201..5011
Gene:CASR
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001178065
Organism Homo sapiens (human)
Definition Homo sapiens calcium-sensing receptor (CASR), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001178065

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGCC ACTCACCTTT
GTGCTGTCTG TCCTCCAGGT GCCCTTCTCC AACTGCAGCC GAGACTGCCT GGCAGGGACC
AGGAAAGGGA TCATTGAGGG GGAGCCCACC TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT
GGGGAGTATA GTGATGAGAC AGATGCCAGT GCCTGTAACA AGTGCCCAGA TGACTTCTGG
TCCAATGAGA ACCACACCTC CTGCATTGCC AAGGAGATCG AGTTTCTGTC GTGGACGGAG
CCCTTTGGGA TCGCACTCAC CCTCTTTGCC GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG
CTGGGTGTGT TTATCAAGTT CCGCAACACA CCCATTGTCA AGGCCACCAA CCGAGAGCTC
TCCTACCTCC TCCTCTTCTC CCTGCTCTGC TGCTTCTCCA GCTCCCTGTT CTTCATCGGG
GAGCCCCAGG ACTGGACGTG CCGCCTGCGC CAGCCGGCCT TTGGCATCAG CTTCGTGCTC
TGCATCTCAT GCATCCTGGT GAAAACCAAC CGTGTCCTCC TGGTGTTTGA GGCCAAGATC
CCCACCAGCT TCCACCGCAA GTGGTGGGGG CTCAACCTGC AGTTCCTGCT GGTTTTCCTC
TGCACCTTCA TGCAGATTGT CATCTGTGTG ATCTGGCTCT ACACCGCGCC CCCCTCAAGC
TACCGCAACC AGGAGCTGGA GGATGAGATC ATCTTCATCA CGTGCCACGA GGGCTCCCTC
ATGGCCCTGG GCTTCCTGAT CGGCTACACC TGCCTGCTGG CTGCCATCTG CTTCTTCTTT
GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC TTCAATGAAG CCAAGTTCAT CACCTTCAGC
ATGCTCATCT TCTTCATCGT CTGGATCTCC TTCATTCCAG CCTATGCCAG CACCTATGGC
AAGTTTGTCT CTGCCGTAGA GGTGATTGCC ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG
TGCATCTTCT TCAACAAGAT CTACATCATT CTCTTCAAGC CATCCCGCAA CACCATCGAG
GAGGTGCGTT GCAGCACCGC AGCTCACGCT TTCAAGGTGG CTGCCCGGGC CACGCTGCGC
CGCAGCAACG TCTCCCGCAA GCGGTCCAGC AGCCTTGGAG GCTCCACGGG ATCCACCCCC
TCCTCCTCCA TCAGCAGCAA GAGCAACAGC GAAGACCCAT TCCCACAGCC CGAGAGGCAG
AAGCAGCAGC AGCCGCTGGC CCTAACCCAG CAAGAGCAGC AGCAGCAGCC CCTGACCCTC
CCACAGCAGC AACGATCTCA GCAGCAGCCC AGATGCAAGC AGAAGGTCAT CTTTGGCAGC
GGCACGGTCA CCTTCTCACT GAGCTTTGAT GAGCCTCAGA AGAACGCCAT GGCCCACAGG
AATTCTACGC ACCAGAACTC CCTGGAGGCC CAGAAAAGCA GCGATACGCT GACCCGACAC
CAGCCATTAC TCCCGCTGCA GTGCGGGGAA ACGGACTTAG ATCTGACCGT CCAGGAAACA
GGTCTGCAAG GACCTGTGGG TGGAGACCAG CGGCCAGAGG TGGAGGACCC TGAAGAGTTG
TCCCCAGCAC TTGTAGTGTC CAGTTCACAG AGCTTTGTCA TCAGTGGTGG AGGCAGCACT
GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27046
Clone ID Related Accession (Same CDS sequence) NM_000388
Accession Version NM_000388.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product extracellular calcium-sensing receptor isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068630.21, U20759.1, U20760.1, AC068754.16 and BP388878.1. This sequence is a reference standard in the RefSeqGene project. On Jun 5, 2008 this sequence version replaced gi:37577158. Transcript Variant: This variant (2) has an alternate 5' UTR exon and lacks an in-frame segment in the CDS, as compared to variant 1. The resulting isoform (2) is shorter and lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U20759.1, D50855.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCCTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC CAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000379.2
CDS373..3609
Misc Feature(1)334..336(+)
Misc Feature(2)430..1962(+)
Misc Feature(3)568..1620(+)
Misc Feature(4)577..1857(+)
Misc Feature(5)640..642(+)
Misc Feature(6)685..1098(+)
Misc Feature(7)760..762(+)
Misc Feature(8)1153..1155(+)
Misc Feature(9)1231..1233(+)
Misc Feature(10)1708..1710(+)
Misc Feature(11)1774..1776(+)
Misc Feature(12)1834..1836(+)
Misc Feature(13)1984..2145(+)
Misc Feature(14)2209..2277(+)
Misc Feature(15)2236..2952(+)
Misc Feature(16)2320..2382(+)
Misc Feature(17)2416..2934(+)
Misc Feature(18)2416..2472(+)
Misc Feature(19)2545..2607(+)
Misc Feature(20)2680..2748(+)
Misc Feature(21)2788..2856(+)
Misc Feature(22)2881..2958(+)
Misc Feature(23)3010..3072(+)
Misc Feature(24)3034..3036(+)
Misc Feature(25)3034..3036(+)
Misc Feature(26)3034..3036(+)
Exon (1)1..130
Gene:CASR
Gene Synonym:
Exon (2)131..557
Gene:CASR
Gene Synonym:
Exon (3)558..864
Gene:CASR
Gene Synonym:
Exon (4)865..1749
Gene:CASR
Gene Synonym:
Exon (5)1750..1980
Gene:CASR
Gene Synonym:
Exon (6)1981..2104
Gene:CASR
Gene Synonym:
Exon (7)2105..4915
Gene:CASR
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000388
Organism Homo sapiens (human)
Definition Homo sapiens calcium-sensing receptor (CASR), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000388

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCCTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC CAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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