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ADGRE3 adhesion G protein-coupled receptor E3 [Homo sapiens (human)]

Gene Symbol ADGRE3
Entrez Gene ID 84658
Full Name adhesion G protein-coupled receptor E3
Synonyms EMR3
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19p13.1

Summary This gene encodes a member of the class B seven-span transmembrane (TM7) receptor family expressed predominantly by cells of the immune system. Family members are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor (EGF)-like domains coupled to a TM7 domain via a mucin-like spacer domain. This gene is closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 2 on chromosome 19. This protein may play a role in myeloid-myeloid interactions during immune and inflammatory responses. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014]. lac of sum
Disorder MIM:

606101

Disorder Html:





The following ADGRE3 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRE3 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu01156 NM_001289158 Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu29077 NM_001289159 Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu05054 NM_032571 Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector in pcDNA3.1+/C-(K)DYK $549
OHu73662 XM_011528374 PREDICTED: Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu51870 XM_005260118 PREDICTED: Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu01156
Accession Version NM_001289158.1
Sequence Information ORF Nucleotide Sequence (Length: 1803bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 29-JUN-2015
Organism Homo sapiens (human)
Product adhesion G protein-coupled receptor E3 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832663.1, AY358817.1, AF239764.1, BC128053.1 and AK298653.1. On Jan 16, 2014 this sequence version replaced gi:530415284. Summary: This gene encodes a member of the class B seven-span transmembrane (TM7) receptor family expressed predominantly by cells of the immune system. Family members are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor (EGF)-like domains coupled to a TM7 domain via a mucin-like spacer domain. This gene is closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 2 on chromosome 19. This protein may play a role in myeloid-myeloid interactions during immune and inflammatory responses. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014]. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter isoform (2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AY358817.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC
CCCTTGGAGA CATGTAACGA CACCACCTCC TCAAAGACAA CCGAGGGCAG GAAAGAGCTG
CAAAAGATTG TGGACAAATT TGAGTCACTT CTCACCAATC AGACTTTATG GAGAACAGAA
GGGAGACAAG AAATCTCATC CACAGCTACC ACTATTCTCC GGGATGTGGA ATCGAAAGTT
CTAGAAACTG CCTTGAAAGA TCCAGAACAA AAAGTCCTGA AAATCCAAAA CGATAGTGTA
GCTATTGAAA CTCAAGCGAT TACAGACAAT TGCTCTGAAG AAAGAAAGAC ATTCAACTTG
AACGTCCAAA TGAACTCAAT GGACATCCGT TGCAGTGACA TCATCCAGGG AGACACACAA
GGTCCCAGTG CCATTGCCTT TATCTCATAT TCTTCTCTTG GAAACATCAT AAATGCAACT
TTTTTTGAAG AGATGGATAA GAAAGATCAA GTGTATCTGA ACTCTCAGGT TGTGAGTGCT
GCTATTGGAC CCAAAAGGAA CGTGTCTCTC TCCAAGTCTG TGACGCTGAC TTTCCAGCAC
GTGAAGATGA CCCCCAGTAC CAAAAAGGTC TTCTGTGTCT ACTGGAAGAG CACAGGGCAG
GGCAGCCAGT GGTCCAGGGA TGGCTGCTTC CTGATACACG TGAACAAGAG TCACACCATG
TGTAATTGCA GTCACCTGTC CAGCTTCGCT GTCCTGATGG CCCTGACCAG CCAGGAGGAG
GATCCCGTGC TGACTGTCAT CACCTACGTG GGGCTGAGCG TCTCTCTGCT GTGCCTCCTC
CTGGCGGCCC TCACTTTTCT CCTGTGTAAA GCCATCCGGA ACACCAGCAC CTCACTGCAT
CTGCAGCTCT CGCTCTGCCT CTTCCTGGCC CACCTCCTCT TCCTCGTGGG GATTGATCGA
ACTGAACCCA AGGTGCTGTG CTCCATCATC GCCGGTGCTT TGCACTATCT CTACCTGGCC
GCCTTCACCT GGATGCTGCT GGAGGGTGTG CACCTCTTCC TCACTGCACG GAACCTGACA
GTGGTCAACT ACTCAAGCAT CAATAGACTC ATGAAGTGGA TCATGTTCCC AGTCGGCTAT
GGCGTTCCCG CTGTGACTGT GGCCATTTCT GCAGCCTCCT GGCCTCACCT TTATGGAACT
GCTGATCGAT GCTGGCTCCA CCTGGACCAG GGATTCATGT GGAGTTTCCT TGGCCCAGTC
TGTGCCATTT TCTCTGCGAA TTTAGTATTG TTTATCTTGG TCTTTTGGAT TTTGAAAAGA
AAACTTTCCT CCCTCAATAG TGAAGTGTCA ACCATCCAGA ACACAAGGAT GCTGGCTTTC
AAAGCAACAG CTCAGCTCTT CATCCTGGGC TGCACATGGT GTCTGGGCTT GCTACAGGTG
GGTCCAGCTG CCCAGGTCAT GGCCTACCTC TTCACCATCA TCAACAGCCT CCAAGGCTTC
TTCATCTTCT TGGTCTACTG CCTCCTCAGC CAGCAGGTCC AGAAACAATA TCAAAAGTGG
TTTAGAGAGA TCGTAAAATC AAAATCTGAG TCTGAGACAT ACACACTTTC CAGCAAGATG
GGTCCTGACT CAAAACCCAG TGAGGGGGAT GTTTTTCCAG GACAAGTGAA GAGAAAATAT
TAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001276087.1
CDS149..1951
Misc Feature(1)74..76(+)
Misc Feature(2)206..>421(+)
Misc Feature(3)902..1027(+)
Misc Feature(4)1049..1774(+)
Exon (1)1..173
Gene:ADGRE3
Gene Synonym:
Exon (2)174..224
Gene:ADGRE3
Gene Synonym:
Exon (3)225..347
Gene:ADGRE3
Gene Synonym:
Exon (4)348..385
Gene:ADGRE3
Gene Synonym:
Exon (5)386..569
Gene:ADGRE3
Gene Synonym:
Exon (6)570..689
Gene:ADGRE3
Gene Synonym:
Exon (7)690..874
Gene:ADGRE3
Gene Synonym:
Exon (8)875..1042
Gene:ADGRE3
Gene Synonym:
Exon (9)1043..1240
Gene:ADGRE3
Gene Synonym:
Exon (10)1241..1476
Gene:ADGRE3
Gene Synonym:
Exon (11)1477..1543
Gene:ADGRE3
Gene Synonym:
Exon (12)1544..1635
Gene:ADGRE3
Gene Synonym:
Exon (13)1636..1804
Gene:ADGRE3
Gene Synonym:
Exon (14)1805..1912
Gene:ADGRE3
Gene Synonym:
Exon (15)1913..2267
Gene:ADGRE3
Gene Synonym:
Translation
Position Chain Variation Link
52 52 a, g dbSNP:567640076
72 72 a, g dbSNP:372686132
94 94 c, t dbSNP:549593199
99 99 c, g dbSNP:527876613
117 117 c, g dbSNP:765545407
121 121 a, g dbSNP:759520903
127 127 a, g, t dbSNP:370548709
132 132 a, t dbSNP:766161692
135 135 c, t dbSNP:760617087
138 138 a, c, g dbSNP:771882447
139 139 a, t dbSNP:761632865
141 141 c, g dbSNP:773965990
148 148 a, g dbSNP:566825917
160 160 a, g dbSNP:551616848
162 162 a, t dbSNP:201487935
168 168 c, t dbSNP:771296383
173 173 c, g dbSNP:181921684
175 175 c, t dbSNP:780176632
181 181 c, t dbSNP:554248680
182 182 c, t dbSNP:750555946
196 196 c, t dbSNP:767379878
221 221 a, g dbSNP:144197422
223 223 -, aa dbSNP:771845884
228 228 c, t dbSNP:778870994
248 248 a, g dbSNP:371686982
251 251 a, g dbSNP:753506465
253 253 c, t dbSNP:766180743
256 256 c, t dbSNP:368054080
258 258 a, g dbSNP:749965366
260 260 a, g dbSNP:764608130
262 262 c, g, t dbSNP:775794636
263 263 a, g dbSNP:531684260
264 264 a, g dbSNP:759944399
269 269 a, g dbSNP:776822310
274 274 -, c dbSNP:760190988
277 277 c, t dbSNP:771126395
294 294 a, t dbSNP:747044399
296 296 a, g dbSNP:201149497
304 304 a, g dbSNP:777147801
307 307 g, t dbSNP:771996069
309 309 a, g dbSNP:148627169
311 311 a, c dbSNP:779032385
314 314 a, g dbSNP:754794750
316 316 a, t dbSNP:749151474
320 320 c, t dbSNP:779747964
324 324 c, t dbSNP:755723062
325 325 a, t dbSNP:749903352
329 329 c, g, t dbSNP:756836391
334 334 a, g dbSNP:202239062
339 339 c, t dbSNP:200473707
344 344 a, g dbSNP:202140919
346 346 c, t dbSNP:545206911
347 347 a, g dbSNP:200584143
351 351 a, c dbSNP:776372463
352 352 c, t dbSNP:576617491
364 364 g, t dbSNP:555064973
365 365 a, c dbSNP:114104678
371 371 c, g dbSNP:4606855
372 372 a, g dbSNP:772836172
374 374 g, t dbSNP:771774285
391 391 a, g dbSNP:779895959
394 394 g, t dbSNP:138910897
395 395 a, g dbSNP:756055657
405 405 a, g dbSNP:183697632
412 412 a, g dbSNP:201504655
413 413 c, t dbSNP:555538254
422 422 a, g dbSNP:111665529
439 439 a, g dbSNP:751214497
441 441 a, g dbSNP:563718426
452 452 a, c dbSNP:765183595
458 458 -, gaa dbSNP:780742328
470 470 a, g dbSNP:775153907
479 479 a, g dbSNP:200661240
481 481 c, t dbSNP:771516221
482 482 a, g dbSNP:761297045
488 488 c, t dbSNP:202129375
489 489 a, g dbSNP:147235913
493 493 c, t dbSNP:191423923
501 501 c, t dbSNP:779190370
502 502 a, g dbSNP:143842724
510 510 g, t dbSNP:749303884
513 513 a, c dbSNP:780126365
518 518 c, g dbSNP:756002517
522 522 -, t dbSNP:756788578
523 523 c, g dbSNP:750327315
526 526 a, c dbSNP:781052118
536 536 c, g, t dbSNP:539327956
551 551 a, t dbSNP:763748027
552 552 c, t dbSNP:568947376
555 555 a, g dbSNP:752215081
559 559 c, t dbSNP:372612623
560 560 a, g, t dbSNP:773754562
562 562 g, t dbSNP:772519761
567 567 a, t dbSNP:762170265
584 584 g, t dbSNP:754529051
585 585 a, c dbSNP:753290906
586 586 a, g dbSNP:112595913
587 587 -, cattgtc dbSNP:754234865
614 614 a, c dbSNP:762245003
617 617 a, t dbSNP:774733912
618 618 c, t dbSNP:764408822
624 624 a, g dbSNP:763354843
631 631 a, c, t dbSNP:201490623
632 632 a, g dbSNP:370544138
653 653 a, g dbSNP:199514629
656 656 -, c dbSNP:766523383
656 656 c, t dbSNP:770861623
657 657 a, g, t dbSNP:777908959
663 663 a, g dbSNP:113225443
667 667 c, t dbSNP:748022045
668 668 a, g dbSNP:778640071
682 682 c, t dbSNP:749056744
686 686 a, c dbSNP:753340170
689 689 a, g, t dbSNP:373403949
699 699 c, t dbSNP:34226397
705 705 c, t dbSNP:760602236
710 710 a, g dbSNP:568804232
716 716 c, t dbSNP:373067590
721 721 c, t dbSNP:772082236
728 728 a, g dbSNP:761869257
732 732 a, g dbSNP:774469284
742 742 g, t dbSNP:768312197
743 743 c, g dbSNP:749164294
744 744 -, c dbSNP:768645591
747 747 c, t dbSNP:779508476
748 748 c, t dbSNP:780999744
754 754 -, t dbSNP:762974450
754 754 c, t dbSNP:745334443
764 764 a, g dbSNP:780712065
774 774 a, g dbSNP:756805250
777 777 a, g dbSNP:753183638
779 779 a, g dbSNP:150299594
783 783 a, g dbSNP:755295994
794 794 c, t dbSNP:369269000
812 812 a, g dbSNP:766450220
816 816 a, g dbSNP:760970194
823 823 a, c dbSNP:750487120
829 829 c, t dbSNP:770652590
830 830 a, g, t dbSNP:200966967
833 833 c, t dbSNP:750730463
834 834 c, t dbSNP:774415927
836 836 c, g dbSNP:550501309
847 847 c, t dbSNP:768703872
852 852 c, t dbSNP:192735238
853 853 -, gctgac dbSNP:775638711
868 868 c, t dbSNP:561414450
869 869 a, g, t dbSNP:375117467
883 883 c, t dbSNP:112147214
884 884 a, t dbSNP:145101518
885 885 a, g dbSNP:775703180
887 887 a, c dbSNP:765063112
901 901 c, t dbSNP:759388329
905 905 g, t dbSNP:776263932
913 913 a, g dbSNP:770578093
920 920 a, g dbSNP:367812454
921 921 c, t dbSNP:772798430
924 924 a, g dbSNP:769000753
926 926 a, c dbSNP:192329346
926 926 -, c dbSNP:767734724
930 930 g, t dbSNP:780474013
931 931 a, c, t dbSNP:746299422
932 932 a, t dbSNP:781495577
945 945 c, g dbSNP:757578330
958 958 c, t dbSNP:149623759
959 959 c, t dbSNP:751554472
960 960 c, t dbSNP:141371829
967 967 c, g dbSNP:758456635
968 968 a, g dbSNP:146634810
986 986 a, g dbSNP:765229625
988 988 a, g dbSNP:537152625
997 997 a, c dbSNP:776426359
1003 1003 c, g dbSNP:765964678
1013 1013 c, t dbSNP:760404622
1015 1015 c, t dbSNP:569609559
1016 1016 a, g dbSNP:760712152
1026 1026 g, t dbSNP:772955743
1029 1029 c, t dbSNP:776060649
1038 1038 a, g dbSNP:554819125
1046 1046 a, g dbSNP:747402538
1048 1048 g, t dbSNP:777989326
1054 1054 c, t dbSNP:144458654
1066 1066 c, t dbSNP:748212943
1074 1074 a, g dbSNP:778992035
1075 1075 c, t dbSNP:141113162
1076 1076 a, g dbSNP:149010185
1083 1083 c, t dbSNP:780020679
1085 1085 a, g dbSNP:755931722
1086 1086 g, t dbSNP:750189823
1087 1087 a, c, t dbSNP:761392728
1088 1088 a, g dbSNP:562575959
1092 1092 c, g dbSNP:763675390
1105 1105 c, t dbSNP:145693240
1108 1108 c, t dbSNP:267605309
1111 1111 a, g dbSNP:201409408
1113 1113 c, t dbSNP:146538980
1114 1114 a, g dbSNP:144211355
1115 1115 a, g dbSNP:773474306
1116 1116 a, c dbSNP:139751044
1127 1127 c, g dbSNP:59183049
1129 1129 c, t dbSNP:202133610
1131 1131 c, t dbSNP:768705620
1140 1140 a, c dbSNP:749436271
1146 1146 a, g dbSNP:45508602
1152 1152 c, t dbSNP:140552399
1161 1161 a, c dbSNP:756017783
1165 1165 a, g dbSNP:745625428
1166 1166 c, t dbSNP:376742705
1170 1170 a, t dbSNP:375614211
1178 1178 c, t dbSNP:757125481
1179 1179 c, t dbSNP:200188037
1180 1180 a, g dbSNP:190395132
1184 1184 c, t dbSNP:757917921
1213 1213 c, t dbSNP:752272423
1214 1214 a, g dbSNP:202195537
1216 1216 c, g dbSNP:761120698
1218 1218 c, g dbSNP:773805888
1219 1219 a, g dbSNP:767747649
1223 1223 c, g, t dbSNP:774549131
1227 1227 a, g dbSNP:377703147
1231 1231 g, t dbSNP:749308035
1235 1235 a, c dbSNP:775452381
1236 1236 a, c dbSNP:769730847
1241 1241 a, g dbSNP:777745398
1258 1258 c, t dbSNP:772035400
1259 1259 a, g dbSNP:747730948
1261 1261 c, t dbSNP:201350463
1262 1262 a, g dbSNP:146799017
1276 1276 a, t dbSNP:753389498
1280 1280 c, t dbSNP:781492965
1288 1288 c, t dbSNP:757784261
1289 1289 a, g dbSNP:200012041
1296 1296 c, t dbSNP:764409272
1318 1318 a, g dbSNP:763367274
1324 1324 c, t dbSNP:752859625
1337 1337 c, t dbSNP:149794291
1338 1338 a, g dbSNP:759479912
1344 1344 c, t dbSNP:560993669
1346 1346 a, c dbSNP:200162165
1357 1357 c, t dbSNP:770954017
1359 1359 a, c dbSNP:760660949
1368 1368 c, t dbSNP:200566145
1373 1373 a, g dbSNP:771976175
1375 1375 a, g dbSNP:572188950
1384 1384 a, g dbSNP:778360655
1386 1386 a, g dbSNP:768366871
1392 1392 c, t dbSNP:143690127
1396 1396 c, t dbSNP:779570553
1402 1402 c, t dbSNP:201863044
1403 1403 a, g dbSNP:553930762
1404 1404 g, t dbSNP:752049041
1407 1407 a, g dbSNP:371350366
1411 1411 c, t dbSNP:778319481
1412 1412 a, g dbSNP:559170756
1413 1413 c, t dbSNP:753119764
1417 1417 c, t dbSNP:367693909
1418 1418 a, g dbSNP:35094696
1440 1440 c, t dbSNP:376296541
1449 1449 a, g, t dbSNP:760891833
1456 1456 c, g, t dbSNP:371645604
1457 1457 c, t dbSNP:182087961
1460 1460 a, t dbSNP:774217514
1462 1462 c, t dbSNP:368642635
1466 1466 a, g dbSNP:144427102
1475 1475 c, t dbSNP:149254238
1476 1476 a, g dbSNP:138445869
1489 1489 c, t dbSNP:753823725
1494 1494 a, g dbSNP:748670693
1518 1518 a, t dbSNP:779350546
1536 1536 c, t dbSNP:376096757
1545 1545 a, c, t dbSNP:769054109
1546 1546 a, g dbSNP:749618477
1552 1552 a, c, g dbSNP:770320312
1558 1558 a, g, t dbSNP:781326971
1560 1560 g, t dbSNP:142461083
1566 1566 c, t dbSNP:751604025
1568 1568 g, t dbSNP:373143666
1577 1577 a, c, g dbSNP:138384322
1586 1586 a, c dbSNP:764996847
1592 1592 a, c dbSNP:759388525
1599 1599 c, g dbSNP:533852689
1603 1603 c, t dbSNP:553905155
1605 1605 a, g dbSNP:760169835
1606 1606 -, t dbSNP:560321303
1619 1619 a, g dbSNP:772617742
1644 1644 c, t dbSNP:566254116
1655 1655 a, g, t dbSNP:375553360
1670 1670 a, g dbSNP:755751535
1671 1671 g, t dbSNP:750128292
1672 1672 c, t dbSNP:767112740
1677 1677 a, g dbSNP:373437727
1681 1681 c, t dbSNP:145814654
1684 1684 a, g dbSNP:765853984
1695 1695 a, g dbSNP:140590698
1696 1696 c, t dbSNP:776892717
1704 1704 a, c dbSNP:771368564
1705 1705 c, g dbSNP:747179542
1713 1713 c, t dbSNP:78700925
1716 1716 c, g dbSNP:143228360
1719 1719 c, t dbSNP:748235809
1735 1735 a, c dbSNP:200055618
1749 1749 -, tca dbSNP:776294995
1756 1756 a, c dbSNP:568667077
1764 1764 a, c, g, t dbSNP:779680624
1770 1770 g, t dbSNP:746602856
1785 1785 -, actgcctcc dbSNP:770561045
1791 1791 -, tcctcagcc dbSNP:779663128
1801 1801 c, g, t dbSNP:747383330
1802 1802 c, t dbSNP:550899495
1810 1810 a, g dbSNP:571110477
1823 1823 a, g dbSNP:374894225
1825 1825 g, t dbSNP:780943563
1827 1827 a, g dbSNP:757002547
1828 1828 g, t dbSNP:751299196
1829 1829 g, t dbSNP:777402584
1835 1835 a, g dbSNP:201958148
1839 1839 c, t dbSNP:757988080
1840 1840 a, c, t dbSNP:147270469
1841 1841 a, g dbSNP:149148345
1857 1857 a, g dbSNP:750804682
1864 1864 a, g dbSNP:368065969
1872 1872 c, t dbSNP:761988614
1873 1873 a, t dbSNP:774602580
1882 1882 a, c dbSNP:762634650
1884 1884 a, t dbSNP:199670503
1885 1885 a, g dbSNP:775223026
1886 1886 a, g dbSNP:537124035
1890 1890 a, g dbSNP:117374816
1900 1900 a, g dbSNP:61729083
1901 1901 a, g dbSNP:139987545
1908 1908 a, g dbSNP:752218548
1909 1909 c, t dbSNP:376729505
1910 1910 a, g dbSNP:770900694
1914 1914 a, g dbSNP:772685365
1916 1916 g, t dbSNP:771783207
1923 1923 a, t dbSNP:747567804
1932 1932 a, g dbSNP:778451446
1933 1933 a, g dbSNP:754528083
1936 1936 a, g dbSNP:746359293
1941 1941 c, g dbSNP:781712544
1949 1949 c, t dbSNP:547510061
1951 1951 a, g dbSNP:751933998
1966 1966 a, g dbSNP:764173430
1969 1969 c, t dbSNP:543774397
1971 1971 a, g dbSNP:758616661
1974 1974 c, t dbSNP:752912536
1978 1978 a, t dbSNP:532095264
1986 1986 c, t dbSNP:759758146
1989 1989 a, g dbSNP:776646057
1992 1992 a, g dbSNP:766436136
1999 1999 c, t dbSNP:760501405
2009 2009 c, g dbSNP:11666584
2018 2018 a, g dbSNP:376637291
2087 2087 a, g dbSNP:2482
2089 2089 g, t dbSNP:527992736
2206 2206 c, t dbSNP:772920229
2212 2212 g, t dbSNP:531369650
2235 2235 c, t dbSNP:767134392

Target ORF information:

RefSeq Version NM_001289158
Organism Homo sapiens (human)
Definition Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001289158

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC
CCCTTGGAGA CATGTAACGA CACCACCTCC TCAAAGACAA CCGAGGGCAG GAAAGAGCTG
CAAAAGATTG TGGACAAATT TGAGTCACTT CTCACCAATC AGACTTTATG GAGAACAGAA
GGGAGACAAG AAATCTCATC CACAGCTACC ACTATTCTCC GGGATGTGGA ATCGAAAGTT
CTAGAAACTG CCTTGAAAGA TCCAGAACAA AAAGTCCTGA AAATCCAAAA CGATAGTGTA
GCTATTGAAA CTCAAGCGAT TACAGACAAT TGCTCTGAAG AAAGAAAGAC ATTCAACTTG
AACGTCCAAA TGAACTCAAT GGACATCCGT TGCAGTGACA TCATCCAGGG AGACACACAA
GGTCCCAGTG CCATTGCCTT TATCTCATAT TCTTCTCTTG GAAACATCAT AAATGCAACT
TTTTTTGAAG AGATGGATAA GAAAGATCAA GTGTATCTGA ACTCTCAGGT TGTGAGTGCT
GCTATTGGAC CCAAAAGGAA CGTGTCTCTC TCCAAGTCTG TGACGCTGAC TTTCCAGCAC
GTGAAGATGA CCCCCAGTAC CAAAAAGGTC TTCTGTGTCT ACTGGAAGAG CACAGGGCAG
GGCAGCCAGT GGTCCAGGGA TGGCTGCTTC CTGATACACG TGAACAAGAG TCACACCATG
TGTAATTGCA GTCACCTGTC CAGCTTCGCT GTCCTGATGG CCCTGACCAG CCAGGAGGAG
GATCCCGTGC TGACTGTCAT CACCTACGTG GGGCTGAGCG TCTCTCTGCT GTGCCTCCTC
CTGGCGGCCC TCACTTTTCT CCTGTGTAAA GCCATCCGGA ACACCAGCAC CTCACTGCAT
CTGCAGCTCT CGCTCTGCCT CTTCCTGGCC CACCTCCTCT TCCTCGTGGG GATTGATCGA
ACTGAACCCA AGGTGCTGTG CTCCATCATC GCCGGTGCTT TGCACTATCT CTACCTGGCC
GCCTTCACCT GGATGCTGCT GGAGGGTGTG CACCTCTTCC TCACTGCACG GAACCTGACA
GTGGTCAACT ACTCAAGCAT CAATAGACTC ATGAAGTGGA TCATGTTCCC AGTCGGCTAT
GGCGTTCCCG CTGTGACTGT GGCCATTTCT GCAGCCTCCT GGCCTCACCT TTATGGAACT
GCTGATCGAT GCTGGCTCCA CCTGGACCAG GGATTCATGT GGAGTTTCCT TGGCCCAGTC
TGTGCCATTT TCTCTGCGAA TTTAGTATTG TTTATCTTGG TCTTTTGGAT TTTGAAAAGA
AAACTTTCCT CCCTCAATAG TGAAGTGTCA ACCATCCAGA ACACAAGGAT GCTGGCTTTC
AAAGCAACAG CTCAGCTCTT CATCCTGGGC TGCACATGGT GTCTGGGCTT GCTACAGGTG
GGTCCAGCTG CCCAGGTCAT GGCCTACCTC TTCACCATCA TCAACAGCCT CCAAGGCTTC
TTCATCTTCT TGGTCTACTG CCTCCTCAGC CAGCAGGTCC AGAAACAATA TCAAAAGTGG
TTTAGAGAGA TCGTAAAATC AAAATCTGAG TCTGAGACAT ACACACTTTC CAGCAAGATG
GGTCCTGACT CAAAACCCAG TGAGGGGGAT GTTTTTCCAG GACAAGTGAA GAGAAAATAT
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu29077
Accession Version NM_001289159.1
Sequence Information ORF Nucleotide Sequence (Length: 1581bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 29-JUN-2015
Organism Homo sapiens (human)
Product adhesion G protein-coupled receptor E3 isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832663.1, AK298653.1, BC128053.1 and AF239764.1. On Jan 16, 2014 this sequence version replaced gi:530415288. Summary: This gene encodes a member of the class B seven-span transmembrane (TM7) receptor family expressed predominantly by cells of the immune system. Family members are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor (EGF)-like domains coupled to a TM7 domain via a mucin-like spacer domain. This gene is closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 2 on chromosome 19. This protein may play a role in myeloid-myeloid interactions during immune and inflammatory responses. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014]. Transcript Variant: This variant (3) lacks three alternate in-frame exons in the 5' coding region, compared to variant 1. This results in a shorter isoform (3), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK298653.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA2142363 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC
CCCTTGGAGA CATGTAACGC TATTGAAACT CAAGCGATTA CAGACAATTG CTCTGAAGAA
AGAAAGACAT TCAACTTGAA CGTCCAAATG AACTCAATGG ACATCCGTTG CAGTGACATC
ATCCAGGGAG ACACACAAGG TCCCAGTGCC ATTGCCTTTA TCTCATATTC TTCTCTTGGA
AACATCATAA ATGCAACTTT TTTTGAAGAG ATGGATAAGA AAGATCAAGT GTATCTGAAC
TCTCAGGTTG TGAGTGCTGC TATTGGACCC AAAAGGAACG TGTCTCTCTC CAAGTCTGTG
ACGCTGACTT TCCAGCACGT GAAGATGACC CCCAGTACCA AAAAGGTCTT CTGTGTCTAC
TGGAAGAGCA CAGGGCAGGG CAGCCAGTGG TCCAGGGATG GCTGCTTCCT GATACACGTG
AACAAGAGTC ACACCATGTG TAATTGCAGT CACCTGTCCA GCTTCGCTGT CCTGATGGCC
CTGACCAGCC AGGAGGAGGA TCCCGTGCTG ACTGTCATCA CCTACGTGGG GCTGAGCGTC
TCTCTGCTGT GCCTCCTCCT GGCGGCCCTC ACTTTTCTCC TGTGTAAAGC CATCCGGAAC
ACCAGCACCT CACTGCATCT GCAGCTCTCG CTCTGCCTCT TCCTGGCCCA CCTCCTCTTC
CTCGTGGGGA TTGATCGAAC TGAACCCAAG GTGCTGTGCT CCATCATCGC CGGTGCTTTG
CACTATCTCT ACCTGGCCGC CTTCACCTGG ATGCTGCTGG AGGGTGTGCA CCTCTTCCTC
ACTGCACGGA ACCTGACAGT GGTCAACTAC TCAAGCATCA ATAGACTCAT GAAGTGGATC
ATGTTCCCAG TCGGCTATGG CGTTCCCGCT GTGACTGTGG CCATTTCTGC AGCCTCCTGG
CCTCACCTTT ATGGAACTGC TGATCGATGC TGGCTCCACC TGGACCAGGG ATTCATGTGG
AGTTTCCTTG GCCCAGTCTG TGCCATTTTC TCTGCGAATT TAGTATTGTT TATCTTGGTC
TTTTGGATTT TGAAAAGAAA ACTTTCCTCC CTCAATAGTG AAGTGTCAAC CATCCAGAAC
ACAAGGATGC TGGCTTTCAA AGCAACAGCT CAGCTCTTCA TCCTGGGCTG CACATGGTGT
CTGGGCTTGC TACAGGTGGG TCCAGCTGCC CAGGTCATGG CCTACCTCTT CACCATCATC
AACAGCCTCC AAGGCTTCTT CATCTTCTTG GTCTACTGCC TCCTCAGCCA GCAGGTCCAG
AAACAATATC AAAAGTGGTT TAGAGAGATC GTAAAATCAA AATCTGAGTC TGAGACATAC
ACACTTTCCA GCAAGATGGG TCCTGACTCA AAACCCAGTG AGGGGGATGT TTTTCCAGGA
CAAGTGAAGA GAAAATATTA A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001276088.1
CDS149..1729
Misc Feature(1)74..76(+)
Misc Feature(2)680..805(+)
Misc Feature(3)827..1552(+)
Exon (1)1..173
Gene:ADGRE3
Gene Synonym:
Exon (2)174..224
Gene:ADGRE3
Gene Synonym:
Exon (3)225..347
Gene:ADGRE3
Gene Synonym:
Exon (4)348..467
Gene:ADGRE3
Gene Synonym:
Exon (5)468..652
Gene:ADGRE3
Gene Synonym:
Exon (6)653..820
Gene:ADGRE3
Gene Synonym:
Exon (7)821..1018
Gene:ADGRE3
Gene Synonym:
Exon (8)1019..1254
Gene:ADGRE3
Gene Synonym:
Exon (9)1255..1321
Gene:ADGRE3
Gene Synonym:
Exon (10)1322..1413
Gene:ADGRE3
Gene Synonym:
Exon (11)1414..1582
Gene:ADGRE3
Gene Synonym:
Exon (12)1583..1690
Gene:ADGRE3
Gene Synonym:
Exon (13)1691..2045
Gene:ADGRE3
Gene Synonym:
Translation
Position Chain Variation Link
52 52 a, g dbSNP:567640076
72 72 a, g dbSNP:372686132
94 94 c, t dbSNP:549593199
99 99 c, g dbSNP:527876613
117 117 c, g dbSNP:765545407
121 121 a, g dbSNP:759520903
127 127 a, g, t dbSNP:370548709
132 132 a, t dbSNP:766161692
135 135 c, t dbSNP:760617087
138 138 a, c, g dbSNP:771882447
139 139 a, t dbSNP:761632865
141 141 c, g dbSNP:773965990
148 148 a, g dbSNP:566825917
160 160 a, g dbSNP:551616848
162 162 a, t dbSNP:201487935
168 168 c, t dbSNP:771296383
173 173 c, g dbSNP:181921684
175 175 c, t dbSNP:780176632
181 181 c, t dbSNP:554248680
182 182 c, t dbSNP:750555946
196 196 c, t dbSNP:767379878
221 221 a, g dbSNP:144197422
223 223 -, aa dbSNP:771845884
228 228 c, t dbSNP:778870994
248 248 a, g dbSNP:371686982
251 251 a, g dbSNP:753506465
253 253 c, t dbSNP:766180743
256 256 c, t dbSNP:368054080
258 258 a, g dbSNP:749965366
260 260 a, g dbSNP:764608130
262 262 c, g, t dbSNP:775794636
263 263 a, g dbSNP:531684260
264 264 a, g dbSNP:759944399
269 269 a, g dbSNP:776822310
274 274 -, c dbSNP:760190988
277 277 c, t dbSNP:771126395
294 294 a, t dbSNP:747044399
296 296 a, g dbSNP:201149497
304 304 a, g dbSNP:777147801
307 307 g, t dbSNP:771996069
309 309 a, g dbSNP:148627169
311 311 a, c dbSNP:779032385
314 314 a, g dbSNP:754794750
316 316 a, t dbSNP:749151474
320 320 c, t dbSNP:779747964
324 324 c, t dbSNP:755723062
325 325 a, t dbSNP:749903352
329 329 c, g, t dbSNP:756836391
334 334 a, g dbSNP:202239062
339 339 c, t dbSNP:200473707
344 344 a, g dbSNP:202140919
346 346 c, t dbSNP:545206911
347 347 a, g dbSNP:200584143
362 362 g, t dbSNP:754529051
363 363 a, c dbSNP:753290906
364 364 a, g dbSNP:112595913
365 365 -, cattgtc dbSNP:754234865
392 392 a, c dbSNP:762245003
395 395 a, t dbSNP:774733912
396 396 c, t dbSNP:764408822
402 402 a, g dbSNP:763354843
409 409 a, c, t dbSNP:201490623
410 410 a, g dbSNP:370544138
431 431 a, g dbSNP:199514629
434 434 -, c dbSNP:766523383
434 434 c, t dbSNP:770861623
435 435 a, g, t dbSNP:777908959
441 441 a, g dbSNP:113225443
445 445 c, t dbSNP:748022045
446 446 a, g dbSNP:778640071
460 460 c, t dbSNP:749056744
464 464 a, c dbSNP:753340170
467 467 a, g, t dbSNP:373403949
477 477 c, t dbSNP:34226397
483 483 c, t dbSNP:760602236
488 488 a, g dbSNP:568804232
494 494 c, t dbSNP:373067590
499 499 c, t dbSNP:772082236
506 506 a, g dbSNP:761869257
510 510 a, g dbSNP:774469284
520 520 g, t dbSNP:768312197
521 521 c, g dbSNP:749164294
522 522 -, c dbSNP:768645591
525 525 c, t dbSNP:779508476
526 526 c, t dbSNP:780999744
532 532 -, t dbSNP:762974450
532 532 c, t dbSNP:745334443
542 542 a, g dbSNP:780712065
552 552 a, g dbSNP:756805250
555 555 a, g dbSNP:753183638
557 557 a, g dbSNP:150299594
561 561 a, g dbSNP:755295994
572 572 c, t dbSNP:369269000
590 590 a, g dbSNP:766450220
594 594 a, g dbSNP:760970194
601 601 a, c dbSNP:750487120
607 607 c, t dbSNP:770652590
608 608 a, g, t dbSNP:200966967
611 611 c, t dbSNP:750730463
612 612 c, t dbSNP:774415927
614 614 c, g dbSNP:550501309
625 625 c, t dbSNP:768703872
630 630 c, t dbSNP:192735238
631 631 -, gctgac dbSNP:775638711
646 646 c, t dbSNP:561414450
647 647 a, g, t dbSNP:375117467
661 661 c, t dbSNP:112147214
662 662 a, t dbSNP:145101518
663 663 a, g dbSNP:775703180
665 665 a, c dbSNP:765063112
679 679 c, t dbSNP:759388329
683 683 g, t dbSNP:776263932
691 691 a, g dbSNP:770578093
698 698 a, g dbSNP:367812454
699 699 c, t dbSNP:772798430
702 702 a, g dbSNP:769000753
704 704 a, c dbSNP:192329346
704 704 -, c dbSNP:767734724
708 708 g, t dbSNP:780474013
709 709 a, c, t dbSNP:746299422
710 710 a, t dbSNP:781495577
723 723 c, g dbSNP:757578330
736 736 c, t dbSNP:149623759
737 737 c, t dbSNP:751554472
738 738 c, t dbSNP:141371829
745 745 c, g dbSNP:758456635
746 746 a, g dbSNP:146634810
764 764 a, g dbSNP:765229625
766 766 a, g dbSNP:537152625
775 775 a, c dbSNP:776426359
781 781 c, g dbSNP:765964678
791 791 c, t dbSNP:760404622
793 793 c, t dbSNP:569609559
794 794 a, g dbSNP:760712152
804 804 g, t dbSNP:772955743
807 807 c, t dbSNP:776060649
816 816 a, g dbSNP:554819125
824 824 a, g dbSNP:747402538
826 826 g, t dbSNP:777989326
832 832 c, t dbSNP:144458654
844 844 c, t dbSNP:748212943
852 852 a, g dbSNP:778992035
853 853 c, t dbSNP:141113162
854 854 a, g dbSNP:149010185
861 861 c, t dbSNP:780020679
863 863 a, g dbSNP:755931722
864 864 g, t dbSNP:750189823
865 865 a, c, t dbSNP:761392728
866 866 a, g dbSNP:562575959
870 870 c, g dbSNP:763675390
883 883 c, t dbSNP:145693240
886 886 c, t dbSNP:267605309
889 889 a, g dbSNP:201409408
891 891 c, t dbSNP:146538980
892 892 a, g dbSNP:144211355
893 893 a, g dbSNP:773474306
894 894 a, c dbSNP:139751044
905 905 c, g dbSNP:59183049
907 907 c, t dbSNP:202133610
909 909 c, t dbSNP:768705620
918 918 a, c dbSNP:749436271
924 924 a, g dbSNP:45508602
930 930 c, t dbSNP:140552399
939 939 a, c dbSNP:756017783
943 943 a, g dbSNP:745625428
944 944 c, t dbSNP:376742705
948 948 a, t dbSNP:375614211
956 956 c, t dbSNP:757125481
957 957 c, t dbSNP:200188037
958 958 a, g dbSNP:190395132
962 962 c, t dbSNP:757917921
991 991 c, t dbSNP:752272423
992 992 a, g dbSNP:202195537
994 994 c, g dbSNP:761120698
996 996 c, g dbSNP:773805888
997 997 a, g dbSNP:767747649
1001 1001 c, g, t dbSNP:774549131
1005 1005 a, g dbSNP:377703147
1009 1009 g, t dbSNP:749308035
1013 1013 a, c dbSNP:775452381
1014 1014 a, c dbSNP:769730847
1019 1019 a, g dbSNP:777745398
1036 1036 c, t dbSNP:772035400
1037 1037 a, g dbSNP:747730948
1039 1039 c, t dbSNP:201350463
1040 1040 a, g dbSNP:146799017
1054 1054 a, t dbSNP:753389498
1058 1058 c, t dbSNP:781492965
1066 1066 c, t dbSNP:757784261
1067 1067 a, g dbSNP:200012041
1074 1074 c, t dbSNP:764409272
1096 1096 a, g dbSNP:763367274
1102 1102 c, t dbSNP:752859625
1115 1115 c, t dbSNP:149794291
1116 1116 a, g dbSNP:759479912
1122 1122 c, t dbSNP:560993669
1124 1124 a, c dbSNP:200162165
1135 1135 c, t dbSNP:770954017
1137 1137 a, c dbSNP:760660949
1146 1146 c, t dbSNP:200566145
1151 1151 a, g dbSNP:771976175
1153 1153 a, g dbSNP:572188950
1162 1162 a, g dbSNP:778360655
1164 1164 a, g dbSNP:768366871
1170 1170 c, t dbSNP:143690127
1174 1174 c, t dbSNP:779570553
1180 1180 c, t dbSNP:201863044
1181 1181 a, g dbSNP:553930762
1182 1182 g, t dbSNP:752049041
1185 1185 a, g dbSNP:371350366
1189 1189 c, t dbSNP:778319481
1190 1190 a, g dbSNP:559170756
1191 1191 c, t dbSNP:753119764
1195 1195 c, t dbSNP:367693909
1196 1196 a, g dbSNP:35094696
1218 1218 c, t dbSNP:376296541
1227 1227 a, g, t dbSNP:760891833
1234 1234 c, g, t dbSNP:371645604
1235 1235 c, t dbSNP:182087961
1238 1238 a, t dbSNP:774217514
1240 1240 c, t dbSNP:368642635
1244 1244 a, g dbSNP:144427102
1253 1253 c, t dbSNP:149254238
1254 1254 a, g dbSNP:138445869
1267 1267 c, t dbSNP:753823725
1272 1272 a, g dbSNP:748670693
1296 1296 a, t dbSNP:779350546
1314 1314 c, t dbSNP:376096757
1323 1323 a, c, t dbSNP:769054109
1324 1324 a, g dbSNP:749618477
1330 1330 a, c, g dbSNP:770320312
1336 1336 a, g, t dbSNP:781326971
1338 1338 g, t dbSNP:142461083
1344 1344 c, t dbSNP:751604025
1346 1346 g, t dbSNP:373143666
1355 1355 a, c, g dbSNP:138384322
1364 1364 a, c dbSNP:764996847
1370 1370 a, c dbSNP:759388525
1377 1377 c, g dbSNP:533852689
1381 1381 c, t dbSNP:553905155
1383 1383 a, g dbSNP:760169835
1384 1384 -, t dbSNP:560321303
1397 1397 a, g dbSNP:772617742
1422 1422 c, t dbSNP:566254116
1433 1433 a, g, t dbSNP:375553360
1448 1448 a, g dbSNP:755751535
1449 1449 g, t dbSNP:750128292
1450 1450 c, t dbSNP:767112740
1455 1455 a, g dbSNP:373437727
1459 1459 c, t dbSNP:145814654
1462 1462 a, g dbSNP:765853984
1473 1473 a, g dbSNP:140590698
1474 1474 c, t dbSNP:776892717
1482 1482 a, c dbSNP:771368564
1483 1483 c, g dbSNP:747179542
1491 1491 c, t dbSNP:78700925
1494 1494 c, g dbSNP:143228360
1497 1497 c, t dbSNP:748235809
1513 1513 a, c dbSNP:200055618
1527 1527 -, tca dbSNP:776294995
1534 1534 a, c dbSNP:568667077
1542 1542 a, c, g, t dbSNP:779680624
1548 1548 g, t dbSNP:746602856
1563 1563 -, actgcctcc dbSNP:770561045
1569 1569 -, tcctcagcc dbSNP:779663128
1579 1579 c, g, t dbSNP:747383330
1580 1580 c, t dbSNP:550899495
1588 1588 a, g dbSNP:571110477
1601 1601 a, g dbSNP:374894225
1603 1603 g, t dbSNP:780943563
1605 1605 a, g dbSNP:757002547
1606 1606 g, t dbSNP:751299196
1607 1607 g, t dbSNP:777402584
1613 1613 a, g dbSNP:201958148
1617 1617 c, t dbSNP:757988080
1618 1618 a, c, t dbSNP:147270469
1619 1619 a, g dbSNP:149148345
1635 1635 a, g dbSNP:750804682
1642 1642 a, g dbSNP:368065969
1650 1650 c, t dbSNP:761988614
1651 1651 a, t dbSNP:774602580
1660 1660 a, c dbSNP:762634650
1662 1662 a, t dbSNP:199670503
1663 1663 a, g dbSNP:775223026
1664 1664 a, g dbSNP:537124035
1668 1668 a, g dbSNP:117374816
1678 1678 a, g dbSNP:61729083
1679 1679 a, g dbSNP:139987545
1686 1686 a, g dbSNP:752218548
1687 1687 c, t dbSNP:376729505
1688 1688 a, g dbSNP:770900694
1692 1692 a, g dbSNP:772685365
1694 1694 g, t dbSNP:771783207
1701 1701 a, t dbSNP:747567804
1710 1710 a, g dbSNP:778451446
1711 1711 a, g dbSNP:754528083
1714 1714 a, g dbSNP:746359293
1719 1719 c, g dbSNP:781712544
1727 1727 c, t dbSNP:547510061
1729 1729 a, g dbSNP:751933998
1744 1744 a, g dbSNP:764173430
1747 1747 c, t dbSNP:543774397
1749 1749 a, g dbSNP:758616661
1752 1752 c, t dbSNP:752912536
1756 1756 a, t dbSNP:532095264
1764 1764 c, t dbSNP:759758146
1767 1767 a, g dbSNP:776646057
1770 1770 a, g dbSNP:766436136
1777 1777 c, t dbSNP:760501405
1787 1787 c, g dbSNP:11666584
1796 1796 a, g dbSNP:376637291
1865 1865 a, g dbSNP:2482
1867 1867 g, t dbSNP:527992736
1984 1984 c, t dbSNP:772920229
1990 1990 g, t dbSNP:531369650
2013 2013 c, t dbSNP:767134392

Target ORF information:

RefSeq Version NM_001289159
Organism Homo sapiens (human)
Definition Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001289159

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC
CCCTTGGAGA CATGTAACGC TATTGAAACT CAAGCGATTA CAGACAATTG CTCTGAAGAA
AGAAAGACAT TCAACTTGAA CGTCCAAATG AACTCAATGG ACATCCGTTG CAGTGACATC
ATCCAGGGAG ACACACAAGG TCCCAGTGCC ATTGCCTTTA TCTCATATTC TTCTCTTGGA
AACATCATAA ATGCAACTTT TTTTGAAGAG ATGGATAAGA AAGATCAAGT GTATCTGAAC
TCTCAGGTTG TGAGTGCTGC TATTGGACCC AAAAGGAACG TGTCTCTCTC CAAGTCTGTG
ACGCTGACTT TCCAGCACGT GAAGATGACC CCCAGTACCA AAAAGGTCTT CTGTGTCTAC
TGGAAGAGCA CAGGGCAGGG CAGCCAGTGG TCCAGGGATG GCTGCTTCCT GATACACGTG
AACAAGAGTC ACACCATGTG TAATTGCAGT CACCTGTCCA GCTTCGCTGT CCTGATGGCC
CTGACCAGCC AGGAGGAGGA TCCCGTGCTG ACTGTCATCA CCTACGTGGG GCTGAGCGTC
TCTCTGCTGT GCCTCCTCCT GGCGGCCCTC ACTTTTCTCC TGTGTAAAGC CATCCGGAAC
ACCAGCACCT CACTGCATCT GCAGCTCTCG CTCTGCCTCT TCCTGGCCCA CCTCCTCTTC
CTCGTGGGGA TTGATCGAAC TGAACCCAAG GTGCTGTGCT CCATCATCGC CGGTGCTTTG
CACTATCTCT ACCTGGCCGC CTTCACCTGG ATGCTGCTGG AGGGTGTGCA CCTCTTCCTC
ACTGCACGGA ACCTGACAGT GGTCAACTAC TCAAGCATCA ATAGACTCAT GAAGTGGATC
ATGTTCCCAG TCGGCTATGG CGTTCCCGCT GTGACTGTGG CCATTTCTGC AGCCTCCTGG
CCTCACCTTT ATGGAACTGC TGATCGATGC TGGCTCCACC TGGACCAGGG ATTCATGTGG
AGTTTCCTTG GCCCAGTCTG TGCCATTTTC TCTGCGAATT TAGTATTGTT TATCTTGGTC
TTTTGGATTT TGAAAAGAAA ACTTTCCTCC CTCAATAGTG AAGTGTCAAC CATCCAGAAC
ACAAGGATGC TGGCTTTCAA AGCAACAGCT CAGCTCTTCA TCCTGGGCTG CACATGGTGT
CTGGGCTTGC TACAGGTGGG TCCAGCTGCC CAGGTCATGG CCTACCTCTT CACCATCATC
AACAGCCTCC AAGGCTTCTT CATCTTCTTG GTCTACTGCC TCCTCAGCCA GCAGGTCCAG
AAACAATATC AAAAGTGGTT TAGAGAGATC GTAAAATCAA AATCTGAGTC TGAGACATAC
ACACTTTCCA GCAAGATGGG TCCTGACTCA AAACCCAGTG AGGGGGATGT TTTTCCAGGA
CAAGTGAAGA GAAAATATTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu05054
Accession Version NM_032571.4
Sequence Information ORF Nucleotide Sequence (Length: 1959bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 29-JUN-2015
Organism Homo sapiens (human)
Product adhesion G protein-coupled receptor E3 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832663.1, BC121130.2, AF239764.1, BC128053.1 and AK298653.1. On Jan 16, 2014 this sequence version replaced gi:133930785. Summary: This gene encodes a member of the class B seven-span transmembrane (TM7) receptor family expressed predominantly by cells of the immune system. Family members are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor (EGF)-like domains coupled to a TM7 domain via a mucin-like spacer domain. This gene is closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 2 on chromosome 19. This protein may play a role in myeloid-myeloid interactions during immune and inflammatory responses. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC142639.1, AF239764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC
CCCTTGGAGA CATGTAACGA CATTAATGAA TGTACACCAC CCTATAGTGT ATATTGTGGA
TTTAACGCTG TGTGTTACAA TGTCGAAGGA AGTTTCTACT GTCAATGTGT CCCAGGATAT
AGACTGCATT CTGGGAATGA ACAATTCAGT AATTCCAATG AGAACACCTG TCAGGACACC
ACCTCCTCAA AGACAACCGA GGGCAGGAAA GAGCTGCAAA AGATTGTGGA CAAATTTGAG
TCACTTCTCA CCAATCAGAC TTTATGGAGA ACAGAAGGGA GACAAGAAAT CTCATCCACA
GCTACCACTA TTCTCCGGGA TGTGGAATCG AAAGTTCTAG AAACTGCCTT GAAAGATCCA
GAACAAAAAG TCCTGAAAAT CCAAAACGAT AGTGTAGCTA TTGAAACTCA AGCGATTACA
GACAATTGCT CTGAAGAAAG AAAGACATTC AACTTGAACG TCCAAATGAA CTCAATGGAC
ATCCGTTGCA GTGACATCAT CCAGGGAGAC ACACAAGGTC CCAGTGCCAT TGCCTTTATC
TCATATTCTT CTCTTGGAAA CATCATAAAT GCAACTTTTT TTGAAGAGAT GGATAAGAAA
GATCAAGTGT ATCTGAACTC TCAGGTTGTG AGTGCTGCTA TTGGACCCAA AAGGAACGTG
TCTCTCTCCA AGTCTGTGAC GCTGACTTTC CAGCACGTGA AGATGACCCC CAGTACCAAA
AAGGTCTTCT GTGTCTACTG GAAGAGCACA GGGCAGGGCA GCCAGTGGTC CAGGGATGGC
TGCTTCCTGA TACACGTGAA CAAGAGTCAC ACCATGTGTA ATTGCAGTCA CCTGTCCAGC
TTCGCTGTCC TGATGGCCCT GACCAGCCAG GAGGAGGATC CCGTGCTGAC TGTCATCACC
TACGTGGGGC TGAGCGTCTC TCTGCTGTGC CTCCTCCTGG CGGCCCTCAC TTTTCTCCTG
TGTAAAGCCA TCCGGAACAC CAGCACCTCA CTGCATCTGC AGCTCTCGCT CTGCCTCTTC
CTGGCCCACC TCCTCTTCCT CGTGGGGATT GATCGAACTG AACCCAAGGT GCTGTGCTCC
ATCATCGCCG GTGCTTTGCA CTATCTCTAC CTGGCCGCCT TCACCTGGAT GCTGCTGGAG
GGTGTGCACC TCTTCCTCAC TGCACGGAAC CTGACAGTGG TCAACTACTC AAGCATCAAT
AGACTCATGA AGTGGATCAT GTTCCCAGTC GGCTATGGCG TTCCCGCTGT GACTGTGGCC
ATTTCTGCAG CCTCCTGGCC TCACCTTTAT GGAACTGCTG ATCGATGCTG GCTCCACCTG
GACCAGGGAT TCATGTGGAG TTTCCTTGGC CCAGTCTGTG CCATTTTCTC TGCGAATTTA
GTATTGTTTA TCTTGGTCTT TTGGATTTTG AAAAGAAAAC TTTCCTCCCT CAATAGTGAA
GTGTCAACCA TCCAGAACAC AAGGATGCTG GCTTTCAAAG CAACAGCTCA GCTCTTCATC
CTGGGCTGCA CATGGTGTCT GGGCTTGCTA CAGGTGGGTC CAGCTGCCCA GGTCATGGCC
TACCTCTTCA CCATCATCAA CAGCCTCCAA GGCTTCTTCA TCTTCTTGGT CTACTGCCTC
CTCAGCCAGC AGGTCCAGAA ACAATATCAA AAGTGGTTTA GAGAGATCGT AAAATCAAAA
TCTGAGTCTG AGACATACAC ACTTTCCAGC AAGATGGGTC CTGACTCAAA ACCCAGTGAG
GGGGATGTTT TTCCAGGACA AGTGAAGAGA AAATATTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_115960.2
CDS149..2107
Misc Feature(1)74..76(+)
Misc Feature(2)347..460(+)
Misc Feature(3)347..409(+)
Misc Feature(4)569..571(+)
Misc Feature(5)1058..1183(+)
Misc Feature(6)1205..1930(+)
Exon (1)1..173
Gene:ADGRE3
Gene Synonym:
Exon (2)174..224
Gene:ADGRE3
Gene Synonym:
Exon (3)225..347
Gene:ADGRE3
Gene Synonym:
Exon (4)348..503
Gene:ADGRE3
Gene Synonym:
Exon (5)504..541
Gene:ADGRE3
Gene Synonym:
Exon (6)542..725
Gene:ADGRE3
Gene Synonym:
Exon (7)726..845
Gene:ADGRE3
Gene Synonym:
Exon (8)846..1030
Gene:ADGRE3
Gene Synonym:
Exon (9)1031..1198
Gene:ADGRE3
Gene Synonym:
Exon (10)1199..1396
Gene:ADGRE3
Gene Synonym:
Exon (11)1397..1632
Gene:ADGRE3
Gene Synonym:
Exon (12)1633..1699
Gene:ADGRE3
Gene Synonym:
Exon (13)1700..1791
Gene:ADGRE3
Gene Synonym:
Exon (14)1792..1960
Gene:ADGRE3
Gene Synonym:
Exon (15)1961..2068
Gene:ADGRE3
Gene Synonym:
Exon (16)2069..2423
Gene:ADGRE3
Gene Synonym:
Translation
Position Chain Variation Link
52 52 a, g dbSNP:567640076
72 72 a, g dbSNP:372686132
94 94 c, t dbSNP:549593199
99 99 c, g dbSNP:527876613
117 117 c, g dbSNP:765545407
121 121 a, g dbSNP:759520903
127 127 a, g, t dbSNP:370548709
132 132 a, t dbSNP:766161692
135 135 c, t dbSNP:760617087
138 138 a, c, g dbSNP:771882447
139 139 a, t dbSNP:761632865
141 141 c, g dbSNP:773965990
148 148 a, g dbSNP:566825917
160 160 a, g dbSNP:551616848
162 162 a, t dbSNP:201487935
168 168 c, t dbSNP:771296383
173 173 c, g dbSNP:181921684
175 175 c, t dbSNP:780176632
181 181 c, t dbSNP:554248680
182 182 c, t dbSNP:750555946
196 196 c, t dbSNP:767379878
221 221 a, g dbSNP:144197422
223 223 -, aa dbSNP:771845884
228 228 c, t dbSNP:778870994
248 248 a, g dbSNP:371686982
251 251 a, g dbSNP:753506465
253 253 c, t dbSNP:766180743
256 256 c, t dbSNP:368054080
258 258 a, g dbSNP:749965366
260 260 a, g dbSNP:764608130
262 262 c, g, t dbSNP:775794636
263 263 a, g dbSNP:531684260
264 264 a, g dbSNP:759944399
269 269 a, g dbSNP:776822310
274 274 -, c dbSNP:760190988
277 277 c, t dbSNP:771126395
294 294 a, t dbSNP:747044399
296 296 a, g dbSNP:201149497
304 304 a, g dbSNP:777147801
307 307 g, t dbSNP:771996069
309 309 a, g dbSNP:148627169
311 311 a, c dbSNP:779032385
314 314 a, g dbSNP:754794750
316 316 a, t dbSNP:749151474
320 320 c, t dbSNP:779747964
324 324 c, t dbSNP:755723062
325 325 a, t dbSNP:749903352
329 329 c, g, t dbSNP:756836391
334 334 a, g dbSNP:202239062
339 339 c, t dbSNP:200473707
344 344 a, g dbSNP:202140919
346 346 c, t dbSNP:545206911
347 347 a, g dbSNP:200584143
348 348 a, g dbSNP:755491337
352 352 g, t dbSNP:754180464
358 358 a, g dbSNP:573459665
359 359 c, t dbSNP:558389180
367 367 a, g dbSNP:761140810
374 374 a, g dbSNP:773283868
375 375 a, g dbSNP:767833404
376 376 c, t dbSNP:761975631
394 394 c, t dbSNP:774575203
395 395 a, g, t dbSNP:763019388
396 396 c, t dbSNP:368262404
400 400 a, g dbSNP:61729084
406 406 a, c dbSNP:769458599
410 410 g, t dbSNP:745737541
412 412 c, t dbSNP:150826128
413 413 a, g, t dbSNP:61729085
430 430 c, t dbSNP:777288767
433 433 a, g dbSNP:375700065
434 434 c, t dbSNP:754268710
441 441 c, g dbSNP:780658522
448 448 c, t dbSNP:756417187
459 459 -, c dbSNP:34172648
462 462 a, g dbSNP:750862448
464 464 a, g dbSNP:767779070
465 465 a, t dbSNP:762137039
478 478 g, t dbSNP:577596349
481 481 c, t dbSNP:142306281
486 486 a, g dbSNP:763178917
488 488 a, g dbSNP:775280358
495 495 c, t dbSNP:371039028
503 503 c, g dbSNP:116952654
507 507 a, c dbSNP:776372463
508 508 c, t dbSNP:576617491
520 520 g, t dbSNP:555064973
521 521 a, c dbSNP:114104678
527 527 c, g dbSNP:4606855
528 528 a, g dbSNP:772836172
530 530 g, t dbSNP:771774285
547 547 a, g dbSNP:779895959
550 550 g, t dbSNP:138910897
551 551 a, g dbSNP:756055657
561 561 a, g dbSNP:183697632
568 568 a, g dbSNP:201504655
569 569 c, t dbSNP:555538254
578 578 a, g dbSNP:111665529
595 595 a, g dbSNP:751214497
597 597 a, g dbSNP:563718426
608 608 a, c dbSNP:765183595
614 614 -, gaa dbSNP:780742328
626 626 a, g dbSNP:775153907
635 635 a, g dbSNP:200661240
637 637 c, t dbSNP:771516221
638 638 a, g dbSNP:761297045
644 644 c, t dbSNP:202129375
645 645 a, g dbSNP:147235913
649 649 c, t dbSNP:191423923
657 657 c, t dbSNP:779190370
658 658 a, g dbSNP:143842724
666 666 g, t dbSNP:749303884
669 669 a, c dbSNP:780126365
674 674 c, g dbSNP:756002517
678 678 -, t dbSNP:756788578
679 679 c, g dbSNP:750327315
682 682 a, c dbSNP:781052118
692 692 c, g, t dbSNP:539327956
707 707 a, t dbSNP:763748027
708 708 c, t dbSNP:568947376
711 711 a, g dbSNP:752215081
715 715 c, t dbSNP:372612623
716 716 a, g, t dbSNP:773754562
718 718 g, t dbSNP:772519761
723 723 a, t dbSNP:762170265
740 740 g, t dbSNP:754529051
741 741 a, c dbSNP:753290906
742 742 a, g dbSNP:112595913
743 743 -, cattgtc dbSNP:754234865
770 770 a, c dbSNP:762245003
773 773 a, t dbSNP:774733912
774 774 c, t dbSNP:764408822
780 780 a, g dbSNP:763354843
787 787 a, c, t dbSNP:201490623
788 788 a, g dbSNP:370544138
809 809 a, g dbSNP:199514629
812 812 -, c dbSNP:766523383
812 812 c, t dbSNP:770861623
813 813 a, g, t dbSNP:777908959
819 819 a, g dbSNP:113225443
823 823 c, t dbSNP:748022045
824 824 a, g dbSNP:778640071
838 838 c, t dbSNP:749056744
842 842 a, c dbSNP:753340170
845 845 a, g, t dbSNP:373403949
855 855 c, t dbSNP:34226397
861 861 c, t dbSNP:760602236
866 866 a, g dbSNP:568804232
872 872 c, t dbSNP:373067590
877 877 c, t dbSNP:772082236
884 884 a, g dbSNP:761869257
888 888 a, g dbSNP:774469284
898 898 g, t dbSNP:768312197
899 899 c, g dbSNP:749164294
900 900 -, c dbSNP:768645591
903 903 c, t dbSNP:779508476
904 904 c, t dbSNP:780999744
910 910 -, t dbSNP:762974450
910 910 c, t dbSNP:745334443
920 920 a, g dbSNP:780712065
930 930 a, g dbSNP:756805250
933 933 a, g dbSNP:753183638
935 935 a, g dbSNP:150299594
939 939 a, g dbSNP:755295994
950 950 c, t dbSNP:369269000
968 968 a, g dbSNP:766450220
972 972 a, g dbSNP:760970194
979 979 a, c dbSNP:750487120
985 985 c, t dbSNP:770652590
986 986 a, g, t dbSNP:200966967
989 989 c, t dbSNP:750730463
990 990 c, t dbSNP:774415927
992 992 c, g dbSNP:550501309
1003 1003 c, t dbSNP:768703872
1008 1008 c, t dbSNP:192735238
1009 1009 -, gctgac dbSNP:775638711
1024 1024 c, t dbSNP:561414450
1025 1025 a, g, t dbSNP:375117467
1039 1039 c, t dbSNP:112147214
1040 1040 a, t dbSNP:145101518
1041 1041 a, g dbSNP:775703180
1043 1043 a, c dbSNP:765063112
1057 1057 c, t dbSNP:759388329
1061 1061 g, t dbSNP:776263932
1069 1069 a, g dbSNP:770578093
1076 1076 a, g dbSNP:367812454
1077 1077 c, t dbSNP:772798430
1080 1080 a, g dbSNP:769000753
1082 1082 a, c dbSNP:192329346
1082 1082 -, c dbSNP:767734724
1086 1086 g, t dbSNP:780474013
1087 1087 a, c, t dbSNP:746299422
1088 1088 a, t dbSNP:781495577
1101 1101 c, g dbSNP:757578330
1114 1114 c, t dbSNP:149623759
1115 1115 c, t dbSNP:751554472
1116 1116 c, t dbSNP:141371829
1123 1123 c, g dbSNP:758456635
1124 1124 a, g dbSNP:146634810
1142 1142 a, g dbSNP:765229625
1144 1144 a, g dbSNP:537152625
1153 1153 a, c dbSNP:776426359
1159 1159 c, g dbSNP:765964678
1169 1169 c, t dbSNP:760404622
1171 1171 c, t dbSNP:569609559
1172 1172 a, g dbSNP:760712152
1182 1182 g, t dbSNP:772955743
1185 1185 c, t dbSNP:776060649
1194 1194 a, g dbSNP:554819125
1202 1202 a, g dbSNP:747402538
1204 1204 g, t dbSNP:777989326
1210 1210 c, t dbSNP:144458654
1222 1222 c, t dbSNP:748212943
1230 1230 a, g dbSNP:778992035
1231 1231 c, t dbSNP:141113162
1232 1232 a, g dbSNP:149010185
1239 1239 c, t dbSNP:780020679
1241 1241 a, g dbSNP:755931722
1242 1242 g, t dbSNP:750189823
1243 1243 a, c, t dbSNP:761392728
1244 1244 a, g dbSNP:562575959
1248 1248 c, g dbSNP:763675390
1261 1261 c, t dbSNP:145693240
1264 1264 c, t dbSNP:267605309
1267 1267 a, g dbSNP:201409408
1269 1269 c, t dbSNP:146538980
1270 1270 a, g dbSNP:144211355
1271 1271 a, g dbSNP:773474306
1272 1272 a, c dbSNP:139751044
1283 1283 c, g dbSNP:59183049
1285 1285 c, t dbSNP:202133610
1287 1287 c, t dbSNP:768705620
1296 1296 a, c dbSNP:749436271
1302 1302 a, g dbSNP:45508602
1308 1308 c, t dbSNP:140552399
1317 1317 a, c dbSNP:756017783
1321 1321 a, g dbSNP:745625428
1322 1322 c, t dbSNP:376742705
1326 1326 a, t dbSNP:375614211
1334 1334 c, t dbSNP:757125481
1335 1335 c, t dbSNP:200188037
1336 1336 a, g dbSNP:190395132
1340 1340 c, t dbSNP:757917921
1369 1369 c, t dbSNP:752272423
1370 1370 a, g dbSNP:202195537
1372 1372 c, g dbSNP:761120698
1374 1374 c, g dbSNP:773805888
1375 1375 a, g dbSNP:767747649
1379 1379 c, g, t dbSNP:774549131
1383 1383 a, g dbSNP:377703147
1387 1387 g, t dbSNP:749308035
1391 1391 a, c dbSNP:775452381
1392 1392 a, c dbSNP:769730847
1397 1397 a, g dbSNP:777745398
1414 1414 c, t dbSNP:772035400
1415 1415 a, g dbSNP:747730948
1417 1417 c, t dbSNP:201350463
1418 1418 a, g dbSNP:146799017
1432 1432 a, t dbSNP:753389498
1436 1436 c, t dbSNP:781492965
1444 1444 c, t dbSNP:757784261
1445 1445 a, g dbSNP:200012041
1452 1452 c, t dbSNP:764409272
1474 1474 a, g dbSNP:763367274
1480 1480 c, t dbSNP:752859625
1493 1493 c, t dbSNP:149794291
1494 1494 a, g dbSNP:759479912
1500 1500 c, t dbSNP:560993669
1502 1502 a, c dbSNP:200162165
1513 1513 c, t dbSNP:770954017
1515 1515 a, c dbSNP:760660949
1524 1524 c, t dbSNP:200566145
1529 1529 a, g dbSNP:771976175
1531 1531 a, g dbSNP:572188950
1540 1540 a, g dbSNP:778360655
1542 1542 a, g dbSNP:768366871
1548 1548 c, t dbSNP:143690127
1552 1552 c, t dbSNP:779570553
1558 1558 c, t dbSNP:201863044
1559 1559 a, g dbSNP:553930762
1560 1560 g, t dbSNP:752049041
1563 1563 a, g dbSNP:371350366
1567 1567 c, t dbSNP:778319481
1568 1568 a, g dbSNP:559170756
1569 1569 c, t dbSNP:753119764
1573 1573 c, t dbSNP:367693909
1574 1574 a, g dbSNP:35094696
1596 1596 c, t dbSNP:376296541
1605 1605 a, g, t dbSNP:760891833
1612 1612 c, g, t dbSNP:371645604
1613 1613 c, t dbSNP:182087961
1616 1616 a, t dbSNP:774217514
1618 1618 c, t dbSNP:368642635
1622 1622 a, g dbSNP:144427102
1631 1631 c, t dbSNP:149254238
1632 1632 a, g dbSNP:138445869
1645 1645 c, t dbSNP:753823725
1650 1650 a, g dbSNP:748670693
1674 1674 a, t dbSNP:779350546
1692 1692 c, t dbSNP:376096757
1701 1701 a, c, t dbSNP:769054109
1702 1702 a, g dbSNP:749618477
1708 1708 a, c, g dbSNP:770320312
1714 1714 a, g, t dbSNP:781326971
1716 1716 g, t dbSNP:142461083
1722 1722 c, t dbSNP:751604025
1724 1724 g, t dbSNP:373143666
1733 1733 a, c, g dbSNP:138384322
1742 1742 a, c dbSNP:764996847
1748 1748 a, c dbSNP:759388525
1755 1755 c, g dbSNP:533852689
1759 1759 c, t dbSNP:553905155
1761 1761 a, g dbSNP:760169835
1762 1762 -, t dbSNP:560321303
1775 1775 a, g dbSNP:772617742
1800 1800 c, t dbSNP:566254116
1811 1811 a, g, t dbSNP:375553360
1826 1826 a, g dbSNP:755751535
1827 1827 g, t dbSNP:750128292
1828 1828 c, t dbSNP:767112740
1833 1833 a, g dbSNP:373437727
1837 1837 c, t dbSNP:145814654
1840 1840 a, g dbSNP:765853984
1851 1851 a, g dbSNP:140590698
1852 1852 c, t dbSNP:776892717
1860 1860 a, c dbSNP:771368564
1861 1861 c, g dbSNP:747179542
1869 1869 c, t dbSNP:78700925
1872 1872 c, g dbSNP:143228360
1875 1875 c, t dbSNP:748235809
1891 1891 a, c dbSNP:200055618
1905 1905 -, tca dbSNP:776294995
1912 1912 a, c dbSNP:568667077
1920 1920 a, c, g, t dbSNP:779680624
1926 1926 g, t dbSNP:746602856
1941 1941 -, actgcctcc dbSNP:770561045
1947 1947 -, tcctcagcc dbSNP:779663128
1957 1957 c, g, t dbSNP:747383330
1958 1958 c, t dbSNP:550899495
1966 1966 a, g dbSNP:571110477
1979 1979 a, g dbSNP:374894225
1981 1981 g, t dbSNP:780943563
1983 1983 a, g dbSNP:757002547
1984 1984 g, t dbSNP:751299196
1985 1985 g, t dbSNP:777402584
1991 1991 a, g dbSNP:201958148
1995 1995 c, t dbSNP:757988080
1996 1996 a, c, t dbSNP:147270469
1997 1997 a, g dbSNP:149148345
2013 2013 a, g dbSNP:750804682
2020 2020 a, g dbSNP:368065969
2028 2028 c, t dbSNP:761988614
2029 2029 a, t dbSNP:774602580
2038 2038 a, c dbSNP:762634650
2040 2040 a, t dbSNP:199670503
2041 2041 a, g dbSNP:775223026
2042 2042 a, g dbSNP:537124035
2046 2046 a, g dbSNP:117374816
2056 2056 a, g dbSNP:61729083
2057 2057 a, g dbSNP:139987545
2064 2064 a, g dbSNP:752218548
2065 2065 c, t dbSNP:376729505
2066 2066 a, g dbSNP:770900694
2070 2070 a, g dbSNP:772685365
2072 2072 g, t dbSNP:771783207
2079 2079 a, t dbSNP:747567804
2088 2088 a, g dbSNP:778451446
2089 2089 a, g dbSNP:754528083
2092 2092 a, g dbSNP:746359293
2097 2097 c, g dbSNP:781712544
2105 2105 c, t dbSNP:547510061
2107 2107 a, g dbSNP:751933998
2122 2122 a, g dbSNP:764173430
2125 2125 c, t dbSNP:543774397
2127 2127 a, g dbSNP:758616661
2130 2130 c, t dbSNP:752912536
2134 2134 a, t dbSNP:532095264
2142 2142 c, t dbSNP:759758146
2145 2145 a, g dbSNP:776646057
2148 2148 a, g dbSNP:766436136
2155 2155 c, t dbSNP:760501405
2165 2165 c, g dbSNP:11666584
2174 2174 a, g dbSNP:376637291
2243 2243 a, g dbSNP:2482
2245 2245 g, t dbSNP:527992736
2362 2362 c, t dbSNP:772920229
2368 2368 g, t dbSNP:531369650
2391 2391 c, t dbSNP:767134392

Target ORF information:

RefSeq Version NM_032571
Organism Homo sapiens (human)
Definition Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_032571

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC
CCCTTGGAGA CATGTAACGA CATTAATGAA TGTACACCAC CCTATAGTGT ATATTGTGGA
TTTAACGCTG TGTGTTACAA TGTCGAAGGA AGTTTCTACT GTCAATGTGT CCCAGGATAT
AGACTGCATT CTGGGAATGA ACAATTCAGT AATTCCAATG AGAACACCTG TCAGGACACC
ACCTCCTCAA AGACAACCGA GGGCAGGAAA GAGCTGCAAA AGATTGTGGA CAAATTTGAG
TCACTTCTCA CCAATCAGAC TTTATGGAGA ACAGAAGGGA GACAAGAAAT CTCATCCACA
GCTACCACTA TTCTCCGGGA TGTGGAATCG AAAGTTCTAG AAACTGCCTT GAAAGATCCA
GAACAAAAAG TCCTGAAAAT CCAAAACGAT AGTGTAGCTA TTGAAACTCA AGCGATTACA
GACAATTGCT CTGAAGAAAG AAAGACATTC AACTTGAACG TCCAAATGAA CTCAATGGAC
ATCCGTTGCA GTGACATCAT CCAGGGAGAC ACACAAGGTC CCAGTGCCAT TGCCTTTATC
TCATATTCTT CTCTTGGAAA CATCATAAAT GCAACTTTTT TTGAAGAGAT GGATAAGAAA
GATCAAGTGT ATCTGAACTC TCAGGTTGTG AGTGCTGCTA TTGGACCCAA AAGGAACGTG
TCTCTCTCCA AGTCTGTGAC GCTGACTTTC CAGCACGTGA AGATGACCCC CAGTACCAAA
AAGGTCTTCT GTGTCTACTG GAAGAGCACA GGGCAGGGCA GCCAGTGGTC CAGGGATGGC
TGCTTCCTGA TACACGTGAA CAAGAGTCAC ACCATGTGTA ATTGCAGTCA CCTGTCCAGC
TTCGCTGTCC TGATGGCCCT GACCAGCCAG GAGGAGGATC CCGTGCTGAC TGTCATCACC
TACGTGGGGC TGAGCGTCTC TCTGCTGTGC CTCCTCCTGG CGGCCCTCAC TTTTCTCCTG
TGTAAAGCCA TCCGGAACAC CAGCACCTCA CTGCATCTGC AGCTCTCGCT CTGCCTCTTC
CTGGCCCACC TCCTCTTCCT CGTGGGGATT GATCGAACTG AACCCAAGGT GCTGTGCTCC
ATCATCGCCG GTGCTTTGCA CTATCTCTAC CTGGCCGCCT TCACCTGGAT GCTGCTGGAG
GGTGTGCACC TCTTCCTCAC TGCACGGAAC CTGACAGTGG TCAACTACTC AAGCATCAAT
AGACTCATGA AGTGGATCAT GTTCCCAGTC GGCTATGGCG TTCCCGCTGT GACTGTGGCC
ATTTCTGCAG CCTCCTGGCC TCACCTTTAT GGAACTGCTG ATCGATGCTG GCTCCACCTG
GACCAGGGAT TCATGTGGAG TTTCCTTGGC CCAGTCTGTG CCATTTTCTC TGCGAATTTA
GTATTGTTTA TCTTGGTCTT TTGGATTTTG AAAAGAAAAC TTTCCTCCCT CAATAGTGAA
GTGTCAACCA TCCAGAACAC AAGGATGCTG GCTTTCAAAG CAACAGCTCA GCTCTTCATC
CTGGGCTGCA CATGGTGTCT GGGCTTGCTA CAGGTGGGTC CAGCTGCCCA GGTCATGGCC
TACCTCTTCA CCATCATCAA CAGCCTCCAA GGCTTCTTCA TCTTCTTGGT CTACTGCCTC
CTCAGCCAGC AGGTCCAGAA ACAATATCAA AAGTGGTTTA GAGAGATCGT AAAATCAAAA
TCTGAGTCTG AGACATACAC ACTTTCCAGC AAGATGGGTC CTGACTCAAA ACCCAGTGAG
GGGGATGTTT TTCCAGGACA AGTGAAGAGA AAATATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu73662
Accession Version XM_011528374.1
Sequence Information ORF Nucleotide Sequence (Length: 1908bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product EGF-like module-containing mucin-like hormone receptor-like 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011295.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGGGAC CATTGCTTCT TCCAGCTTCC TGTGCTAAGT GCCCCCCAAA TGCTTCCTGT 
GTCAATAACA CTCACTGCAC CTGCAACCAT GGATATACTT CTGGATCTGG GCAGAAACTA
TTCACATTCC CCTTGGAGAC ATGTAACGAC ATTAATGAAT GTACACCACC CTATAGTGTA
TATTGTGGAT TTAACGCTGT GTGTTACAAT GTCGAAGGAA GTTTCTACTG TCAATGTGTC
CCAGGATATA GACTGCATTC TGGGAATGAA CAATTCAGTA ATTCCAATGA GAACACCTGT
CAGGACACCA CCTCCTCAAA GACAACCGAG GGCAGGAAAG AGCTGCAAAA GATTGTGGAC
AAATTTGAGT CACTTCTCAC CAATCAGACT TTATGGAGAA CAGAAGGGAG ACAAGAAATC
TCATCCACAG CTACCACTAT TCTCCGGGAT GTGGAATCGA AAGTTCTAGA AACTGCCTTG
AAAGATCCAG AACAAAAAGT CCTGAAAATC CAAAACGATA GTGTAGCTAT TGAAACTCAA
GCGATTACAG ACAATTGCTC TGAAGAAAGA AAGACATTCA ACTTGAACGT CCAAATGAAC
TCAATGGACA TCCGTTGCAG TGACATCATC CAGGGAGACA CACAAGGTCC CAGTGCCATT
GCCTTTATCT CATATTCTTC TCTTGGAAAC ATCATAAATG CAACTTTTTT TGAAGAGATG
GATAAGAAAG ATCAAGTGTA TCTGAACTCT CAGGTTGTGA GTGCTGCTAT TGGACCCAAA
AGGAACGTGT CTCTCTCCAA GTCTGTGACG CTGACTTTCC AGCACGTGAA GATGACCCCC
AGTACCAAAA AGGTCTTCTG TGTCTACTGG AAGAGCACAG GGCAGGGCAG CCAGTGGTCC
AGGGATGGCT GCTTCCTGAT ACACGTGAAC AAGAGTCACA CCATGTGTAA TTGCAGTCAC
CTGTCCAGCT TCGCTGTCCT GATGGCCCTG ACCAGCCAGG AGGAGGATCC CGTGCTGACT
GTCATCACCT ACGTGGGGCT GAGCGTCTCT CTGCTGTGCC TCCTCCTGGC GGCCCTCACT
TTTCTCCTGT GTAAAGCCAT CCGGAACACC AGCACCTCAC TGCATCTGCA GCTCTCGCTC
TGCCTCTTCC TGGCCCACCT CCTCTTCCTC GTGGGGATTG ATCGAACTGA ACCCAAGGTG
CTGTGCTCCA TCATCGCCGG TGCTTTGCAC TATCTCTACC TGGCCGCCTT CACCTGGATG
CTGCTGGAGG GTGTGCACCT CTTCCTCACT GCACGGAACC TGACAGTGGT CAACTACTCA
AGCATCAATA GACTCATGAA GTGGATCATG TTCCCAGTCG GCTATGGCGT TCCCGCTGTG
ACTGTGGCCA TTTCTGCAGC CTCCTGGCCT CACCTTTATG GAACTGCTGA TCGATGCTGG
CTCCACCTGG ACCAGGGATT CATGTGGAGT TTCCTTGGCC CAGTCTGTGC CATTTTCTCT
GCGAATTTAG TATTGTTTAT CTTGGTCTTT TGGATTTTGA AAAGAAAACT TTCCTCCCTC
AATAGTGAAG TGTCAACCAT CCAGAACACA AGGATGCTGG CTTTCAAAGC AACAGCTCAG
CTCTTCATCC TGGGCTGCAC ATGGTGTCTG GGCTTGCTAC AGGTGGGTCC AGCTGCCCAG
GTCATGGCCT ACCTCTTCAC CATCATCAAC AGCCTCCAAG GCTTCTTCAT CTTCTTGGTC
TACTGCCTCC TCAGCCAGCA GGTCCAGAAA CAATATCAAA AGTGGTTTAG AGAGATCGTA
AAATCAAAAT CTGAGTCTGA GACATACACA CTTTCCAGCA AGATGGGTCC TGACTCAAAA
CCCAGTGAGG GGGATGTTTT TCCAGGACAA GTGAAGAGAA AATATTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011526676.1
CDS131..2038
Misc Feature(1)278..391(+)
Misc Feature(2)278..340(+)
Misc Feature(3)989..1114(+)
Misc Feature(4)1136..1861(+)
Translation
Position Chain Variation Link
34 34 a, g dbSNP:567640076
54 54 a, g dbSNP:372686132
76 76 c, t dbSNP:549593199
81 81 c, g dbSNP:527876613
99 99 c, g dbSNP:765545407
103 103 a, g dbSNP:759520903
109 109 a, g, t dbSNP:370548709
114 114 a, t dbSNP:766161692
117 117 c, t dbSNP:760617087
120 120 a, c, g dbSNP:771882447
121 121 a, t dbSNP:761632865
123 123 c, g dbSNP:773965990
130 130 a, g dbSNP:566825917
142 142 a, g dbSNP:551616848
144 144 a, t dbSNP:201487935
150 150 c, t dbSNP:771296383
155 155 c, g dbSNP:181921684
159 159 c, t dbSNP:778870994
179 179 a, g dbSNP:371686982
182 182 a, g dbSNP:753506465
184 184 c, t dbSNP:766180743
187 187 c, t dbSNP:368054080
189 189 a, g dbSNP:749965366
191 191 a, g dbSNP:764608130
193 193 c, g, t dbSNP:775794636
194 194 a, g dbSNP:531684260
195 195 a, g dbSNP:759944399
200 200 a, g dbSNP:776822310
205 205 -, c dbSNP:760190988
208 208 c, t dbSNP:771126395
225 225 a, t dbSNP:747044399
227 227 a, g dbSNP:201149497
235 235 a, g dbSNP:777147801
238 238 g, t dbSNP:771996069
240 240 a, g dbSNP:148627169
242 242 a, c dbSNP:779032385
245 245 a, g dbSNP:754794750
247 247 a, t dbSNP:749151474
251 251 c, t dbSNP:779747964
255 255 c, t dbSNP:755723062
256 256 a, t dbSNP:749903352
260 260 c, g, t dbSNP:756836391
265 265 a, g dbSNP:202239062
270 270 c, t dbSNP:200473707
275 275 a, g dbSNP:202140919
277 277 c, t dbSNP:545206911
278 278 a, g dbSNP:200584143
279 279 a, g dbSNP:755491337
283 283 g, t dbSNP:754180464
289 289 a, g dbSNP:573459665
290 290 c, t dbSNP:558389180
298 298 a, g dbSNP:761140810
305 305 a, g dbSNP:773283868
306 306 a, g dbSNP:767833404
307 307 c, t dbSNP:761975631
325 325 c, t dbSNP:774575203
326 326 a, g, t dbSNP:763019388
327 327 c, t dbSNP:368262404
331 331 a, g dbSNP:61729084
337 337 a, c dbSNP:769458599
341 341 g, t dbSNP:745737541
343 343 c, t dbSNP:150826128
344 344 a, g, t dbSNP:61729085
361 361 c, t dbSNP:777288767
364 364 a, g dbSNP:375700065
365 365 c, t dbSNP:754268710
372 372 c, g dbSNP:780658522
379 379 c, t dbSNP:756417187
390 390 -, c dbSNP:34172648
393 393 a, g dbSNP:750862448
395 395 a, g dbSNP:767779070
396 396 a, t dbSNP:762137039
409 409 g, t dbSNP:577596349
412 412 c, t dbSNP:142306281
417 417 a, g dbSNP:763178917
419 419 a, g dbSNP:775280358
426 426 c, t dbSNP:371039028
434 434 c, g dbSNP:116952654
438 438 a, c dbSNP:776372463
439 439 c, t dbSNP:576617491
451 451 g, t dbSNP:555064973
452 452 a, c dbSNP:114104678
458 458 c, g dbSNP:4606855
459 459 a, g dbSNP:772836172
461 461 g, t dbSNP:771774285
478 478 a, g dbSNP:779895959
481 481 g, t dbSNP:138910897
482 482 a, g dbSNP:756055657
492 492 a, g dbSNP:183697632
499 499 a, g dbSNP:201504655
500 500 c, t dbSNP:555538254
509 509 a, g dbSNP:111665529
526 526 a, g dbSNP:751214497
528 528 a, g dbSNP:563718426
539 539 a, c dbSNP:765183595
545 545 -, gaa dbSNP:780742328
557 557 a, g dbSNP:775153907
566 566 a, g dbSNP:200661240
568 568 c, t dbSNP:771516221
569 569 a, g dbSNP:761297045
575 575 c, t dbSNP:202129375
576 576 a, g dbSNP:147235913
580 580 c, t dbSNP:191423923
588 588 c, t dbSNP:779190370
589 589 a, g dbSNP:143842724
597 597 g, t dbSNP:749303884
600 600 a, c dbSNP:780126365
605 605 c, g dbSNP:756002517
609 609 -, t dbSNP:756788578
610 610 c, g dbSNP:750327315
613 613 a, c dbSNP:781052118
623 623 c, g, t dbSNP:539327956
638 638 a, t dbSNP:763748027
639 639 c, t dbSNP:568947376
642 642 a, g dbSNP:752215081
646 646 c, t dbSNP:372612623
647 647 a, g, t dbSNP:773754562
649 649 g, t dbSNP:772519761
654 654 a, t dbSNP:762170265
671 671 g, t dbSNP:754529051
672 672 a, c dbSNP:753290906
673 673 a, g dbSNP:112595913
674 674 -, cattgtc dbSNP:754234865
701 701 a, c dbSNP:762245003
704 704 a, t dbSNP:774733912
705 705 c, t dbSNP:764408822
711 711 a, g dbSNP:763354843
718 718 a, c, t dbSNP:201490623
719 719 a, g dbSNP:370544138
740 740 a, g dbSNP:199514629
743 743 -, c dbSNP:766523383
743 743 c, t dbSNP:770861623
744 744 a, g, t dbSNP:777908959
750 750 a, g dbSNP:113225443
754 754 c, t dbSNP:748022045
755 755 a, g dbSNP:778640071
769 769 c, t dbSNP:749056744
773 773 a, c dbSNP:753340170
776 776 a, g, t dbSNP:373403949
786 786 c, t dbSNP:34226397
792 792 c, t dbSNP:760602236
797 797 a, g dbSNP:568804232
803 803 c, t dbSNP:373067590
808 808 c, t dbSNP:772082236
815 815 a, g dbSNP:761869257
819 819 a, g dbSNP:774469284
829 829 g, t dbSNP:768312197
830 830 c, g dbSNP:749164294
831 831 -, c dbSNP:768645591
834 834 c, t dbSNP:779508476
835 835 c, t dbSNP:780999744
841 841 -, t dbSNP:762974450
841 841 c, t dbSNP:745334443
851 851 a, g dbSNP:780712065
861 861 a, g dbSNP:756805250
864 864 a, g dbSNP:753183638
866 866 a, g dbSNP:150299594
870 870 a, g dbSNP:755295994
881 881 c, t dbSNP:369269000
899 899 a, g dbSNP:766450220
903 903 a, g dbSNP:760970194
910 910 a, c dbSNP:750487120
916 916 c, t dbSNP:770652590
917 917 a, g, t dbSNP:200966967
920 920 c, t dbSNP:750730463
921 921 c, t dbSNP:774415927
923 923 c, g dbSNP:550501309
934 934 c, t dbSNP:768703872
939 939 c, t dbSNP:192735238
940 940 -, gctgac dbSNP:775638711
955 955 c, t dbSNP:561414450
956 956 a, g, t dbSNP:375117467
970 970 c, t dbSNP:112147214
971 971 a, t dbSNP:145101518
972 972 a, g dbSNP:775703180
974 974 a, c dbSNP:765063112
988 988 c, t dbSNP:759388329
992 992 g, t dbSNP:776263932
1000 1000 a, g dbSNP:770578093
1007 1007 a, g dbSNP:367812454
1008 1008 c, t dbSNP:772798430
1011 1011 a, g dbSNP:769000753
1013 1013 a, c dbSNP:192329346
1013 1013 -, c dbSNP:767734724
1017 1017 g, t dbSNP:780474013
1018 1018 a, c, t dbSNP:746299422
1019 1019 a, t dbSNP:781495577
1032 1032 c, g dbSNP:757578330
1045 1045 c, t dbSNP:149623759
1046 1046 c, t dbSNP:751554472
1047 1047 c, t dbSNP:141371829
1054 1054 c, g dbSNP:758456635
1055 1055 a, g dbSNP:146634810
1073 1073 a, g dbSNP:765229625
1075 1075 a, g dbSNP:537152625
1084 1084 a, c dbSNP:776426359
1090 1090 c, g dbSNP:765964678
1100 1100 c, t dbSNP:760404622
1102 1102 c, t dbSNP:569609559
1103 1103 a, g dbSNP:760712152
1113 1113 g, t dbSNP:772955743
1116 1116 c, t dbSNP:776060649
1125 1125 a, g dbSNP:554819125
1133 1133 a, g dbSNP:747402538
1135 1135 g, t dbSNP:777989326
1141 1141 c, t dbSNP:144458654
1153 1153 c, t dbSNP:748212943
1161 1161 a, g dbSNP:778992035
1162 1162 c, t dbSNP:141113162
1163 1163 a, g dbSNP:149010185
1170 1170 c, t dbSNP:780020679
1172 1172 a, g dbSNP:755931722
1173 1173 g, t dbSNP:750189823
1174 1174 a, c, t dbSNP:761392728
1175 1175 a, g dbSNP:562575959
1179 1179 c, g dbSNP:763675390
1192 1192 c, t dbSNP:145693240
1195 1195 c, t dbSNP:267605309
1198 1198 a, g dbSNP:201409408
1200 1200 c, t dbSNP:146538980
1201 1201 a, g dbSNP:144211355
1202 1202 a, g dbSNP:773474306
1203 1203 a, c dbSNP:139751044
1214 1214 c, g dbSNP:59183049
1216 1216 c, t dbSNP:202133610
1218 1218 c, t dbSNP:768705620
1227 1227 a, c dbSNP:749436271
1233 1233 a, g dbSNP:45508602
1239 1239 c, t dbSNP:140552399
1248 1248 a, c dbSNP:756017783
1252 1252 a, g dbSNP:745625428
1253 1253 c, t dbSNP:376742705
1257 1257 a, t dbSNP:375614211
1265 1265 c, t dbSNP:757125481
1266 1266 c, t dbSNP:200188037
1267 1267 a, g dbSNP:190395132
1271 1271 c, t dbSNP:757917921
1300 1300 c, t dbSNP:752272423
1301 1301 a, g dbSNP:202195537
1303 1303 c, g dbSNP:761120698
1305 1305 c, g dbSNP:773805888
1306 1306 a, g dbSNP:767747649
1310 1310 c, g, t dbSNP:774549131
1314 1314 a, g dbSNP:377703147
1318 1318 g, t dbSNP:749308035
1322 1322 a, c dbSNP:775452381
1323 1323 a, c dbSNP:769730847
1328 1328 a, g dbSNP:777745398
1345 1345 c, t dbSNP:772035400
1346 1346 a, g dbSNP:747730948
1348 1348 c, t dbSNP:201350463
1349 1349 a, g dbSNP:146799017
1363 1363 a, t dbSNP:753389498
1367 1367 c, t dbSNP:781492965
1375 1375 c, t dbSNP:757784261
1376 1376 a, g dbSNP:200012041
1383 1383 c, t dbSNP:764409272
1405 1405 a, g dbSNP:763367274
1411 1411 c, t dbSNP:752859625
1424 1424 c, t dbSNP:149794291
1425 1425 a, g dbSNP:759479912
1431 1431 c, t dbSNP:560993669
1433 1433 a, c dbSNP:200162165
1444 1444 c, t dbSNP:770954017
1446 1446 a, c dbSNP:760660949
1455 1455 c, t dbSNP:200566145
1460 1460 a, g dbSNP:771976175
1462 1462 a, g dbSNP:572188950
1471 1471 a, g dbSNP:778360655
1473 1473 a, g dbSNP:768366871
1479 1479 c, t dbSNP:143690127
1483 1483 c, t dbSNP:779570553
1489 1489 c, t dbSNP:201863044
1490 1490 a, g dbSNP:553930762
1491 1491 g, t dbSNP:752049041
1494 1494 a, g dbSNP:371350366
1498 1498 c, t dbSNP:778319481
1499 1499 a, g dbSNP:559170756
1500 1500 c, t dbSNP:753119764
1504 1504 c, t dbSNP:367693909
1505 1505 a, g dbSNP:35094696
1527 1527 c, t dbSNP:376296541
1536 1536 a, g, t dbSNP:760891833
1543 1543 c, g, t dbSNP:371645604
1544 1544 c, t dbSNP:182087961
1547 1547 a, t dbSNP:774217514
1549 1549 c, t dbSNP:368642635
1553 1553 a, g dbSNP:144427102
1562 1562 c, t dbSNP:149254238
1563 1563 a, g dbSNP:138445869
1576 1576 c, t dbSNP:753823725
1581 1581 a, g dbSNP:748670693
1605 1605 a, t dbSNP:779350546
1623 1623 c, t dbSNP:376096757
1632 1632 a, c, t dbSNP:769054109
1633 1633 a, g dbSNP:749618477
1639 1639 a, c, g dbSNP:770320312
1645 1645 a, g, t dbSNP:781326971
1647 1647 g, t dbSNP:142461083
1653 1653 c, t dbSNP:751604025
1655 1655 g, t dbSNP:373143666
1664 1664 a, c, g dbSNP:138384322
1673 1673 a, c dbSNP:764996847
1679 1679 a, c dbSNP:759388525
1686 1686 c, g dbSNP:533852689
1690 1690 c, t dbSNP:553905155
1692 1692 a, g dbSNP:760169835
1693 1693 -, t dbSNP:560321303
1706 1706 a, g dbSNP:772617742
1731 1731 c, t dbSNP:566254116
1742 1742 a, g, t dbSNP:375553360
1757 1757 a, g dbSNP:755751535
1758 1758 g, t dbSNP:750128292
1759 1759 c, t dbSNP:767112740
1764 1764 a, g dbSNP:373437727
1768 1768 c, t dbSNP:145814654
1771 1771 a, g dbSNP:765853984
1782 1782 a, g dbSNP:140590698
1783 1783 c, t dbSNP:776892717
1791 1791 a, c dbSNP:771368564
1792 1792 c, g dbSNP:747179542
1800 1800 c, t dbSNP:78700925
1803 1803 c, g dbSNP:143228360
1806 1806 c, t dbSNP:748235809
1822 1822 a, c dbSNP:200055618
1836 1836 -, tca dbSNP:776294995
1843 1843 a, c dbSNP:568667077
1851 1851 a, c, g, t dbSNP:779680624
1857 1857 g, t dbSNP:746602856
1872 1872 -, actgcctcc dbSNP:770561045
1878 1878 -, tcctcagcc dbSNP:779663128
1888 1888 c, g, t dbSNP:747383330
1889 1889 c, t dbSNP:550899495
1897 1897 a, g dbSNP:571110477
1910 1910 a, g dbSNP:374894225
1912 1912 g, t dbSNP:780943563
1914 1914 a, g dbSNP:757002547
1915 1915 g, t dbSNP:751299196
1916 1916 g, t dbSNP:777402584
1922 1922 a, g dbSNP:201958148
1926 1926 c, t dbSNP:757988080
1927 1927 a, c, t dbSNP:147270469
1928 1928 a, g dbSNP:149148345
1944 1944 a, g dbSNP:750804682
1951 1951 a, g dbSNP:368065969
1959 1959 c, t dbSNP:761988614
1960 1960 a, t dbSNP:774602580
1969 1969 a, c dbSNP:762634650
1971 1971 a, t dbSNP:199670503
1972 1972 a, g dbSNP:775223026
1973 1973 a, g dbSNP:537124035
1977 1977 a, g dbSNP:117374816
1987 1987 a, g dbSNP:61729083
1988 1988 a, g dbSNP:139987545
1995 1995 a, g dbSNP:752218548
1996 1996 c, t dbSNP:376729505
1997 1997 a, g dbSNP:770900694
2001 2001 a, g dbSNP:772685365
2003 2003 g, t dbSNP:771783207
2010 2010 a, t dbSNP:747567804
2019 2019 a, g dbSNP:778451446
2020 2020 a, g dbSNP:754528083
2023 2023 a, g dbSNP:746359293
2028 2028 c, g dbSNP:781712544
2036 2036 c, t dbSNP:547510061
2038 2038 a, g dbSNP:751933998
2053 2053 a, g dbSNP:764173430
2056 2056 c, t dbSNP:543774397
2058 2058 a, g dbSNP:758616661
2061 2061 c, t dbSNP:752912536
2065 2065 a, t dbSNP:532095264
2073 2073 c, t dbSNP:759758146
2076 2076 a, g dbSNP:776646057
2079 2079 a, g dbSNP:766436136
2086 2086 c, t dbSNP:760501405
2096 2096 c, g dbSNP:11666584
2105 2105 a, g dbSNP:376637291
2174 2174 a, g dbSNP:2482
2176 2176 g, t dbSNP:527992736

Target ORF information:

RefSeq Version XM_011528374
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011528374

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGGGAC CATTGCTTCT TCCAGCTTCC TGTGCTAAGT GCCCCCCAAA TGCTTCCTGT 
GTCAATAACA CTCACTGCAC CTGCAACCAT GGATATACTT CTGGATCTGG GCAGAAACTA
TTCACATTCC CCTTGGAGAC ATGTAACGAC ATTAATGAAT GTACACCACC CTATAGTGTA
TATTGTGGAT TTAACGCTGT GTGTTACAAT GTCGAAGGAA GTTTCTACTG TCAATGTGTC
CCAGGATATA GACTGCATTC TGGGAATGAA CAATTCAGTA ATTCCAATGA GAACACCTGT
CAGGACACCA CCTCCTCAAA GACAACCGAG GGCAGGAAAG AGCTGCAAAA GATTGTGGAC
AAATTTGAGT CACTTCTCAC CAATCAGACT TTATGGAGAA CAGAAGGGAG ACAAGAAATC
TCATCCACAG CTACCACTAT TCTCCGGGAT GTGGAATCGA AAGTTCTAGA AACTGCCTTG
AAAGATCCAG AACAAAAAGT CCTGAAAATC CAAAACGATA GTGTAGCTAT TGAAACTCAA
GCGATTACAG ACAATTGCTC TGAAGAAAGA AAGACATTCA ACTTGAACGT CCAAATGAAC
TCAATGGACA TCCGTTGCAG TGACATCATC CAGGGAGACA CACAAGGTCC CAGTGCCATT
GCCTTTATCT CATATTCTTC TCTTGGAAAC ATCATAAATG CAACTTTTTT TGAAGAGATG
GATAAGAAAG ATCAAGTGTA TCTGAACTCT CAGGTTGTGA GTGCTGCTAT TGGACCCAAA
AGGAACGTGT CTCTCTCCAA GTCTGTGACG CTGACTTTCC AGCACGTGAA GATGACCCCC
AGTACCAAAA AGGTCTTCTG TGTCTACTGG AAGAGCACAG GGCAGGGCAG CCAGTGGTCC
AGGGATGGCT GCTTCCTGAT ACACGTGAAC AAGAGTCACA CCATGTGTAA TTGCAGTCAC
CTGTCCAGCT TCGCTGTCCT GATGGCCCTG ACCAGCCAGG AGGAGGATCC CGTGCTGACT
GTCATCACCT ACGTGGGGCT GAGCGTCTCT CTGCTGTGCC TCCTCCTGGC GGCCCTCACT
TTTCTCCTGT GTAAAGCCAT CCGGAACACC AGCACCTCAC TGCATCTGCA GCTCTCGCTC
TGCCTCTTCC TGGCCCACCT CCTCTTCCTC GTGGGGATTG ATCGAACTGA ACCCAAGGTG
CTGTGCTCCA TCATCGCCGG TGCTTTGCAC TATCTCTACC TGGCCGCCTT CACCTGGATG
CTGCTGGAGG GTGTGCACCT CTTCCTCACT GCACGGAACC TGACAGTGGT CAACTACTCA
AGCATCAATA GACTCATGAA GTGGATCATG TTCCCAGTCG GCTATGGCGT TCCCGCTGTG
ACTGTGGCCA TTTCTGCAGC CTCCTGGCCT CACCTTTATG GAACTGCTGA TCGATGCTGG
CTCCACCTGG ACCAGGGATT CATGTGGAGT TTCCTTGGCC CAGTCTGTGC CATTTTCTCT
GCGAATTTAG TATTGTTTAT CTTGGTCTTT TGGATTTTGA AAAGAAAACT TTCCTCCCTC
AATAGTGAAG TGTCAACCAT CCAGAACACA AGGATGCTGG CTTTCAAAGC AACAGCTCAG
CTCTTCATCC TGGGCTGCAC ATGGTGTCTG GGCTTGCTAC AGGTGGGTCC AGCTGCCCAG
GTCATGGCCT ACCTCTTCAC CATCATCAAC AGCCTCCAAG GCTTCTTCAT CTTCTTGGTC
TACTGCCTCC TCAGCCAGCA GGTCCAGAAA CAATATCAAA AGTGGTTTAG AGAGATCGTA
AAATCAAAAT CTGAGTCTGA GACATACACA CTTTCCAGCA AGATGGGTCC TGACTCAAAA
CCCAGTGAGG GGGATGTTTT TCCAGGACAA GTGAAGAGAA AATATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51870
Accession Version XM_005260118.1
Sequence Information ORF Nucleotide Sequence (Length: 1761bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product EGF-like module-containing mucin-like hormone receptor-like 3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011295.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC
CCCTTGGAGA CATGTAACGA CATTAATGAA TGTACACCAC CCTATAGTGT ATATTGTGGA
TTTAACGCTG TGTGTTACAA TGTCGAAGGA AGTTTCTACT GTCAATGTGT CCCAGGATAT
AGACTGCATT CTGGGAATGA ACAATTCAGT AATTCCAATG AGAACACCTG TCAGGACACC
ACCTCCTCAA AGACAACCGA GGGCAGGAAA GAGCTGCAAA AGATTGTGGA CAAATTTGAG
TCACTTCTCA CCAATCAGAC TTTATGGAGA ACAGAAGGGA GACAAGAAAT CTCATCCACA
GCTACCACTA TTCTCCGGGA TGTGGAATCG AAAGTTCTAG AAACTGCCTT GAAAGATCCA
GAACAAAAAG TCCTGAAAAT CCAAAACGAT AGTGTAGCTA TTGAAACTCA AGCGATTACA
GACAATTGCT CTGAAGAAAG AAAGACATTC AACTTGAACG TCCAAATGAA CTCAATGGAC
ATCCGTTGCA GTGACATCAT CCAGGGAGAC ACACAAGGTC CCAGTGCCAT TGCCTTTATC
TCATATTCTT CTCTTGGAAA CATCATAAAT GCAACTTTTT TTGAAGAGAT GGATAAGAAA
GATCAAGTGT ATCTGAACTC TCAGGTTGTG AGTGCTGCTA TTGGACCCAA AAGGAACGTG
TCTCTCTCCA AGTCTGTGAC GCTGACTTTC CAGCACGTGA AGATGACCCC CAGTACCAAA
AAGGTCTTCT GTGTCTACTG GAAGAGCACA GGGCAGGGCA GCCAGTGGTC CAGGGATGGC
TGCTTCCTGA TACACGTGAA CAAGAGTCAC ACCATGTGTA ATTGCAGTCA CCTGTCCAGC
TTCGCTGTCC TGATGGCCCT GACCAGCCAG GTGCTGTGCT CCATCATCGC CGGTGCTTTG
CACTATCTCT ACCTGGCCGC CTTCACCTGG ATGCTGCTGG AGGGTGTGCA CCTCTTCCTC
ACTGCACGGA ACCTGACAGT GGTCAACTAC TCAAGCATCA ATAGACTCAT GAAGTGGATC
ATGTTCCCAG TCGGCTATGG CGTTCCCGCT GTGACTGTGG CCATTTCTGC AGCCTCCTGG
CCTCACCTTT ATGGAACTGC TGATCGATGC TGGCTCCACC TGGACCAGGG ATTCATGTGG
AGTTTCCTTG GCCCAGTCTG TGCCATTTTC TCTGCGAATT TAGTATTGTT TATCTTGGTC
TTTTGGATTT TGAAAAGAAA ACTTTCCTCC CTCAATAGTG AAGTGTCAAC CATCCAGAAC
ACAAGGATGC TGGCTTTCAA AGCAACAGCT CAGCTCTTCA TCCTGGGCTG CACATGGTGT
CTGGGCTTGC TACAGGTGGG TCCAGCTGCC CAGGTCATGG CCTACCTCTT CACCATCATC
AACAGCCTCC AAGGCTTCTT CATCTTCTTG GTCTACTGCC TCCTCAGCCA GCAGGTCCAG
AAACAATATC AAAAGTGGTT TAGAGAGATC GTAAAATCAA AATCTGAGTC TGAGACATAC
ACACTTTCCA GCAAGATGGG TCCTGACTCA AAACCCAGTG AGGGGGATGT TTTTCCAGGA
CAAGTGAAGA GAAAATATTA A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005260175.1
CDS80..1840
Misc Feature(1)278..391(+)
Misc Feature(2)278..340(+)
Misc Feature(3)1049..1663(+)
Translation
Position Chain Variation Link
3 3 a, g dbSNP:372686132
25 25 c, t dbSNP:549593199
30 30 c, g dbSNP:527876613
48 48 c, g dbSNP:765545407
52 52 a, g dbSNP:759520903
58 58 a, g, t dbSNP:370548709
63 63 a, t dbSNP:766161692
66 66 c, t dbSNP:760617087
69 69 a, c, g dbSNP:771882447
70 70 a, t dbSNP:761632865
72 72 c, g dbSNP:773965990
79 79 a, g dbSNP:566825917
91 91 a, g dbSNP:551616848
93 93 a, t dbSNP:201487935
99 99 c, t dbSNP:771296383
104 104 c, g dbSNP:181921684
106 106 c, t dbSNP:780176632
112 112 c, t dbSNP:554248680
113 113 c, t dbSNP:750555946
127 127 c, t dbSNP:767379878
152 152 a, g dbSNP:144197422
154 154 -, aa dbSNP:771845884
159 159 c, t dbSNP:778870994
179 179 a, g dbSNP:371686982
182 182 a, g dbSNP:753506465
184 184 c, t dbSNP:766180743
187 187 c, t dbSNP:368054080
189 189 a, g dbSNP:749965366
191 191 a, g dbSNP:764608130
193 193 c, g, t dbSNP:775794636
194 194 a, g dbSNP:531684260
195 195 a, g dbSNP:759944399
200 200 a, g dbSNP:776822310
205 205 -, c dbSNP:760190988
208 208 c, t dbSNP:771126395
225 225 a, t dbSNP:747044399
227 227 a, g dbSNP:201149497
235 235 a, g dbSNP:777147801
238 238 g, t dbSNP:771996069
240 240 a, g dbSNP:148627169
242 242 a, c dbSNP:779032385
245 245 a, g dbSNP:754794750
247 247 a, t dbSNP:749151474
251 251 c, t dbSNP:779747964
255 255 c, t dbSNP:755723062
256 256 a, t dbSNP:749903352
260 260 c, g, t dbSNP:756836391
265 265 a, g dbSNP:202239062
270 270 c, t dbSNP:200473707
275 275 a, g dbSNP:202140919
277 277 c, t dbSNP:545206911
278 278 a, g dbSNP:200584143
279 279 a, g dbSNP:755491337
283 283 g, t dbSNP:754180464
289 289 a, g dbSNP:573459665
290 290 c, t dbSNP:558389180
298 298 a, g dbSNP:761140810
305 305 a, g dbSNP:773283868
306 306 a, g dbSNP:767833404
307 307 c, t dbSNP:761975631
325 325 c, t dbSNP:774575203
326 326 a, g, t dbSNP:763019388
327 327 c, t dbSNP:368262404
331 331 a, g dbSNP:61729084
337 337 a, c dbSNP:769458599
341 341 g, t dbSNP:745737541
343 343 c, t dbSNP:150826128
344 344 a, g, t dbSNP:61729085
361 361 c, t dbSNP:777288767
364 364 a, g dbSNP:375700065
365 365 c, t dbSNP:754268710
372 372 c, g dbSNP:780658522
379 379 c, t dbSNP:756417187
390 390 -, c dbSNP:34172648
393 393 a, g dbSNP:750862448
395 395 a, g dbSNP:767779070
396 396 a, t dbSNP:762137039
409 409 g, t dbSNP:577596349
412 412 c, t dbSNP:142306281
417 417 a, g dbSNP:763178917
419 419 a, g dbSNP:775280358
426 426 c, t dbSNP:371039028
434 434 c, g dbSNP:116952654
438 438 a, c dbSNP:776372463
439 439 c, t dbSNP:576617491
451 451 g, t dbSNP:555064973
452 452 a, c dbSNP:114104678
458 458 c, g dbSNP:4606855
459 459 a, g dbSNP:772836172
461 461 g, t dbSNP:771774285
478 478 a, g dbSNP:779895959
481 481 g, t dbSNP:138910897
482 482 a, g dbSNP:756055657
492 492 a, g dbSNP:183697632
499 499 a, g dbSNP:201504655
500 500 c, t dbSNP:555538254
509 509 a, g dbSNP:111665529
526 526 a, g dbSNP:751214497
528 528 a, g dbSNP:563718426
539 539 a, c dbSNP:765183595
545 545 -, gaa dbSNP:780742328
557 557 a, g dbSNP:775153907
566 566 a, g dbSNP:200661240
568 568 c, t dbSNP:771516221
569 569 a, g dbSNP:761297045
575 575 c, t dbSNP:202129375
576 576 a, g dbSNP:147235913
580 580 c, t dbSNP:191423923
588 588 c, t dbSNP:779190370
589 589 a, g dbSNP:143842724
597 597 g, t dbSNP:749303884
600 600 a, c dbSNP:780126365
605 605 c, g dbSNP:756002517
609 609 -, t dbSNP:756788578
610 610 c, g dbSNP:750327315
613 613 a, c dbSNP:781052118
623 623 c, g, t dbSNP:539327956
638 638 a, t dbSNP:763748027
639 639 c, t dbSNP:568947376
642 642 a, g dbSNP:752215081
646 646 c, t dbSNP:372612623
647 647 a, g, t dbSNP:773754562
649 649 g, t dbSNP:772519761
654 654 a, t dbSNP:762170265
671 671 g, t dbSNP:754529051
672 672 a, c dbSNP:753290906
673 673 a, g dbSNP:112595913
674 674 -, cattgtc dbSNP:754234865
701 701 a, c dbSNP:762245003
704 704 a, t dbSNP:774733912
705 705 c, t dbSNP:764408822
711 711 a, g dbSNP:763354843
718 718 a, c, t dbSNP:201490623
719 719 a, g dbSNP:370544138
740 740 a, g dbSNP:199514629
743 743 -, c dbSNP:766523383
743 743 c, t dbSNP:770861623
744 744 a, g, t dbSNP:777908959
750 750 a, g dbSNP:113225443
754 754 c, t dbSNP:748022045
755 755 a, g dbSNP:778640071
769 769 c, t dbSNP:749056744
773 773 a, c dbSNP:753340170
776 776 a, g, t dbSNP:373403949
786 786 c, t dbSNP:34226397
792 792 c, t dbSNP:760602236
797 797 a, g dbSNP:568804232
803 803 c, t dbSNP:373067590
808 808 c, t dbSNP:772082236
815 815 a, g dbSNP:761869257
819 819 a, g dbSNP:774469284
829 829 g, t dbSNP:768312197
830 830 c, g dbSNP:749164294
831 831 -, c dbSNP:768645591
834 834 c, t dbSNP:779508476
835 835 c, t dbSNP:780999744
841 841 -, t dbSNP:762974450
841 841 c, t dbSNP:745334443
851 851 a, g dbSNP:780712065
861 861 a, g dbSNP:756805250
864 864 a, g dbSNP:753183638
866 866 a, g dbSNP:150299594
870 870 a, g dbSNP:755295994
881 881 c, t dbSNP:369269000
899 899 a, g dbSNP:766450220
903 903 a, g dbSNP:760970194
910 910 a, c dbSNP:750487120
916 916 c, t dbSNP:770652590
917 917 a, g, t dbSNP:200966967
920 920 c, t dbSNP:750730463
921 921 c, t dbSNP:774415927
923 923 c, g dbSNP:550501309
934 934 c, t dbSNP:768703872
939 939 c, t dbSNP:192735238
940 940 -, gctgac dbSNP:775638711
955 955 c, t dbSNP:561414450
956 956 a, g, t dbSNP:375117467
970 970 c, t dbSNP:112147214
971 971 a, t dbSNP:145101518
972 972 a, g dbSNP:775703180
974 974 a, c dbSNP:765063112
988 988 c, t dbSNP:759388329
992 992 g, t dbSNP:776263932
1000 1000 a, g dbSNP:770578093
1007 1007 a, g dbSNP:367812454
1008 1008 c, t dbSNP:772798430
1011 1011 a, g dbSNP:769000753
1013 1013 a, c dbSNP:192329346
1013 1013 -, c dbSNP:767734724
1017 1017 g, t dbSNP:780474013
1018 1018 a, c, t dbSNP:746299422
1019 1019 a, t dbSNP:781495577
1032 1032 c, g dbSNP:757578330
1045 1045 c, t dbSNP:149623759
1046 1046 c, t dbSNP:751554472
1047 1047 c, t dbSNP:141371829
1054 1054 c, g dbSNP:758456635
1055 1055 a, g dbSNP:146634810
1073 1073 a, g dbSNP:765229625
1075 1075 a, g dbSNP:537152625
1084 1084 a, c dbSNP:776426359
1090 1090 c, g dbSNP:765964678
1100 1100 c, t dbSNP:760404622
1102 1102 c, t dbSNP:569609559
1103 1103 a, g dbSNP:760712152
1113 1113 g, t dbSNP:772955743
1116 1116 c, t dbSNP:776060649
1125 1125 a, g dbSNP:554819125
1130 1130 a, g dbSNP:777745398
1147 1147 c, t dbSNP:772035400
1148 1148 a, g dbSNP:747730948
1150 1150 c, t dbSNP:201350463
1151 1151 a, g dbSNP:146799017
1165 1165 a, t dbSNP:753389498
1169 1169 c, t dbSNP:781492965
1177 1177 c, t dbSNP:757784261
1178 1178 a, g dbSNP:200012041
1185 1185 c, t dbSNP:764409272
1207 1207 a, g dbSNP:763367274
1213 1213 c, t dbSNP:752859625
1226 1226 c, t dbSNP:149794291
1227 1227 a, g dbSNP:759479912
1233 1233 c, t dbSNP:560993669
1235 1235 a, c dbSNP:200162165
1246 1246 c, t dbSNP:770954017
1248 1248 a, c dbSNP:760660949
1257 1257 c, t dbSNP:200566145
1262 1262 a, g dbSNP:771976175
1264 1264 a, g dbSNP:572188950
1273 1273 a, g dbSNP:778360655
1275 1275 a, g dbSNP:768366871
1281 1281 c, t dbSNP:143690127
1285 1285 c, t dbSNP:779570553
1291 1291 c, t dbSNP:201863044
1292 1292 a, g dbSNP:553930762
1293 1293 g, t dbSNP:752049041
1296 1296 a, g dbSNP:371350366
1300 1300 c, t dbSNP:778319481
1301 1301 a, g dbSNP:559170756
1302 1302 c, t dbSNP:753119764
1306 1306 c, t dbSNP:367693909
1307 1307 a, g dbSNP:35094696
1329 1329 c, t dbSNP:376296541
1338 1338 a, g, t dbSNP:760891833
1345 1345 c, g, t dbSNP:371645604
1346 1346 c, t dbSNP:182087961
1349 1349 a, t dbSNP:774217514
1351 1351 c, t dbSNP:368642635
1355 1355 a, g dbSNP:144427102
1364 1364 c, t dbSNP:149254238
1365 1365 a, g dbSNP:138445869
1378 1378 c, t dbSNP:753823725
1383 1383 a, g dbSNP:748670693
1407 1407 a, t dbSNP:779350546
1425 1425 c, t dbSNP:376096757
1434 1434 a, c, t dbSNP:769054109
1435 1435 a, g dbSNP:749618477
1441 1441 a, c, g dbSNP:770320312
1447 1447 a, g, t dbSNP:781326971
1449 1449 g, t dbSNP:142461083
1455 1455 c, t dbSNP:751604025
1457 1457 g, t dbSNP:373143666
1466 1466 a, c, g dbSNP:138384322
1475 1475 a, c dbSNP:764996847
1481 1481 a, c dbSNP:759388525
1488 1488 c, g dbSNP:533852689
1492 1492 c, t dbSNP:553905155
1494 1494 a, g dbSNP:760169835
1495 1495 -, t dbSNP:560321303
1508 1508 a, g dbSNP:772617742
1533 1533 c, t dbSNP:566254116
1544 1544 a, g, t dbSNP:375553360
1559 1559 a, g dbSNP:755751535
1560 1560 g, t dbSNP:750128292
1561 1561 c, t dbSNP:767112740
1566 1566 a, g dbSNP:373437727
1570 1570 c, t dbSNP:145814654
1573 1573 a, g dbSNP:765853984
1584 1584 a, g dbSNP:140590698
1585 1585 c, t dbSNP:776892717
1593 1593 a, c dbSNP:771368564
1594 1594 c, g dbSNP:747179542
1602 1602 c, t dbSNP:78700925
1605 1605 c, g dbSNP:143228360
1608 1608 c, t dbSNP:748235809
1624 1624 a, c dbSNP:200055618
1638 1638 -, tca dbSNP:776294995
1645 1645 a, c dbSNP:568667077
1653 1653 a, c, g, t dbSNP:779680624
1659 1659 g, t dbSNP:746602856
1674 1674 -, actgcctcc dbSNP:770561045
1680 1680 -, tcctcagcc dbSNP:779663128
1690 1690 c, g, t dbSNP:747383330
1691 1691 c, t dbSNP:550899495
1699 1699 a, g dbSNP:571110477
1712 1712 a, g dbSNP:374894225
1714 1714 g, t dbSNP:780943563
1716 1716 a, g dbSNP:757002547
1717 1717 g, t dbSNP:751299196
1718 1718 g, t dbSNP:777402584
1724 1724 a, g dbSNP:201958148
1728 1728 c, t dbSNP:757988080
1729 1729 a, c, t dbSNP:147270469
1730 1730 a, g dbSNP:149148345
1746 1746 a, g dbSNP:750804682
1753 1753 a, g dbSNP:368065969
1761 1761 c, t dbSNP:761988614
1762 1762 a, t dbSNP:774602580
1771 1771 a, c dbSNP:762634650
1773 1773 a, t dbSNP:199670503
1774 1774 a, g dbSNP:775223026
1775 1775 a, g dbSNP:537124035
1779 1779 a, g dbSNP:117374816
1789 1789 a, g dbSNP:61729083
1790 1790 a, g dbSNP:139987545
1797 1797 a, g dbSNP:752218548
1798 1798 c, t dbSNP:376729505
1799 1799 a, g dbSNP:770900694
1803 1803 a, g dbSNP:772685365
1805 1805 g, t dbSNP:771783207
1812 1812 a, t dbSNP:747567804
1821 1821 a, g dbSNP:778451446
1822 1822 a, g dbSNP:754528083
1825 1825 a, g dbSNP:746359293
1830 1830 c, g dbSNP:781712544
1838 1838 c, t dbSNP:547510061
1840 1840 a, g dbSNP:751933998
1855 1855 a, g dbSNP:764173430
1858 1858 c, t dbSNP:543774397
1860 1860 a, g dbSNP:758616661
1863 1863 c, t dbSNP:752912536
1867 1867 a, t dbSNP:532095264
1875 1875 c, t dbSNP:759758146
1878 1878 a, g dbSNP:776646057
1881 1881 a, g dbSNP:766436136
1888 1888 c, t dbSNP:760501405
1898 1898 c, g dbSNP:11666584
1907 1907 a, g dbSNP:376637291
1976 1976 a, g dbSNP:2482
1978 1978 g, t dbSNP:527992736

Target ORF information:

RefSeq Version XM_005260118
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens adhesion G protein-coupled receptor E3 (ADGRE3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260118

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG 
ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC
ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC
CCCTTGGAGA CATGTAACGA CATTAATGAA TGTACACCAC CCTATAGTGT ATATTGTGGA
TTTAACGCTG TGTGTTACAA TGTCGAAGGA AGTTTCTACT GTCAATGTGT CCCAGGATAT
AGACTGCATT CTGGGAATGA ACAATTCAGT AATTCCAATG AGAACACCTG TCAGGACACC
ACCTCCTCAA AGACAACCGA GGGCAGGAAA GAGCTGCAAA AGATTGTGGA CAAATTTGAG
TCACTTCTCA CCAATCAGAC TTTATGGAGA ACAGAAGGGA GACAAGAAAT CTCATCCACA
GCTACCACTA TTCTCCGGGA TGTGGAATCG AAAGTTCTAG AAACTGCCTT GAAAGATCCA
GAACAAAAAG TCCTGAAAAT CCAAAACGAT AGTGTAGCTA TTGAAACTCA AGCGATTACA
GACAATTGCT CTGAAGAAAG AAAGACATTC AACTTGAACG TCCAAATGAA CTCAATGGAC
ATCCGTTGCA GTGACATCAT CCAGGGAGAC ACACAAGGTC CCAGTGCCAT TGCCTTTATC
TCATATTCTT CTCTTGGAAA CATCATAAAT GCAACTTTTT TTGAAGAGAT GGATAAGAAA
GATCAAGTGT ATCTGAACTC TCAGGTTGTG AGTGCTGCTA TTGGACCCAA AAGGAACGTG
TCTCTCTCCA AGTCTGTGAC GCTGACTTTC CAGCACGTGA AGATGACCCC CAGTACCAAA
AAGGTCTTCT GTGTCTACTG GAAGAGCACA GGGCAGGGCA GCCAGTGGTC CAGGGATGGC
TGCTTCCTGA TACACGTGAA CAAGAGTCAC ACCATGTGTA ATTGCAGTCA CCTGTCCAGC
TTCGCTGTCC TGATGGCCCT GACCAGCCAG GTGCTGTGCT CCATCATCGC CGGTGCTTTG
CACTATCTCT ACCTGGCCGC CTTCACCTGG ATGCTGCTGG AGGGTGTGCA CCTCTTCCTC
ACTGCACGGA ACCTGACAGT GGTCAACTAC TCAAGCATCA ATAGACTCAT GAAGTGGATC
ATGTTCCCAG TCGGCTATGG CGTTCCCGCT GTGACTGTGG CCATTTCTGC AGCCTCCTGG
CCTCACCTTT ATGGAACTGC TGATCGATGC TGGCTCCACC TGGACCAGGG ATTCATGTGG
AGTTTCCTTG GCCCAGTCTG TGCCATTTTC TCTGCGAATT TAGTATTGTT TATCTTGGTC
TTTTGGATTT TGAAAAGAAA ACTTTCCTCC CTCAATAGTG AAGTGTCAAC CATCCAGAAC
ACAAGGATGC TGGCTTTCAA AGCAACAGCT CAGCTCTTCA TCCTGGGCTG CACATGGTGT
CTGGGCTTGC TACAGGTGGG TCCAGCTGCC CAGGTCATGG CCTACCTCTT CACCATCATC
AACAGCCTCC AAGGCTTCTT CATCTTCTTG GTCTACTGCC TCCTCAGCCA GCAGGTCCAG
AAACAATATC AAAAGTGGTT TAGAGAGATC GTAAAATCAA AATCTGAGTC TGAGACATAC
ACACTTTCCA GCAAGATGGG TCCTGACTCA AAACCCAGTG AGGGGGATGT TTTTCCAGGA
CAAGTGAAGA GAAAATATTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.