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HES7 hes family bHLH transcription factor 7 [Homo sapiens (human)]

Gene Symbol HES7
Entrez Gene ID 84667
Full Name hes family bHLH transcription factor 7
Synonyms SCDO4, bHLHb37
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17p13.1

Summary This gene encodes a member of the hairy and enhancer of split family of bHLH transcription factors. The mouse ortholog of this gene is regulated by Notch signaling. The protein functions as a transcriptional repressor, and is implicated in correct patterning of the axial skeleton. A mutation in this gene has been shown to result in spondylocostal dysostosis. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]. lac of sum
Disorder MIM:

608059

Disorder Html: Spondylocostal dysostosis 4, autosomal recessive, 613686 (3)





The following HES7 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HES7 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22579 NM_032580 Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $269
OHu73665 XM_011524038 PREDICTED: Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199
OHu73666 XM_011524039 PREDICTED: Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199
OHu73666 XM_011524040 PREDICTED: Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199
OHu73667 XM_011524041 PREDICTED: Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199
OHu73668 XM_011524042 PREDICTED: Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199
OHu22574 NM_001165967 Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $269

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**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu22579
Accession Version NM_032580.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 678bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-MAY-2014
Organism Homo sapiens (human)
Product transcription factor HES-7 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI259590.1, AB049064.1, AC129492.6 and BC039887.1. This sequence is a reference standard in the RefSeqGene project. On Oct 1, 2009 this sequence version replaced gi:145207993. Summary: This gene encodes a member of the hairy and enhancer of split family of bHLH transcription factors. The mouse ortholog of this gene is regulated by Notch signaling. The protein functions as a transcriptional repressor, and is implicated in correct patterning of the axial skeleton. A mutation in this gene has been shown to result in spondylocostal dysostosis. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]. Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The resulting isoform (2) lacks a five amino acid segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB049064.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
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121
181
241
301
361
421
481
541
601
661
ATGGTCACCC GGGATCGAGC TGAGAATAGG GACGGCCCCA AGATGCTCAA GCCGCTTGTG 
GAGAAGCGGC GCCGGGACCG CATCAACCGC AGCCTGGAAG AGCTGAGGCT GCTGCTGCTG
GAGCGGACCC GGGACCAGAA CCTCCGGAAC CCGAAGCTGG AGAAAGCGGA GATATTGGAG
TTCGCCGTGG GCTACTTGAG GGAGCGAAGC CGGGTGGAGC CCCCGGGGGT TCCCCGGTCC
CCAGTCCAGG ACGCCGAGGC GCTCGCCAGC TGCTACTTGT CCGGTTTCCG CGAGTGCCTG
CTTCGCTTGG CGGCCTTCGC GCACGACGCC AGCCCGGCCG CCCGCGCCCA GCTCTTCTCC
GCGCTGCACG GCTATCTGCG CCCCAAACCG CCCCGGCCCA AGCCGGTAGA TCCGAGGCCT
CCAGCGCCGC GCCCATCCCT GGACCCCGCC GCACCGGCCC TTGGCCCTGC GCTGCACCAG
CGCCCCCCAG TGCACCAGGG CCACCCTAGC CCGCGCTGCG CATGGTCCCC ATCCCTCTGC
TCCCCGCGCG CCGGGGATTC TGGCGCGCCG GCGCCCCTCA CCGGACTGCT GCCGCCGCCA
CCGCCGCCTC ACAGACAAGA CGGGGCGCCC AAGGCCCCGC TGCCCCCGCC GCCCGCTTTC
TGGAGACCTT GGCCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_115969.2
CDS9..686
Misc Feature(1)48..227(+)
Misc Feature(2)48..173(+)
Misc Feature(3)69..71(+)
Misc Feature(4)87..227(+)
Misc Feature(5)516..518(+)
Misc Feature(6)669..680(+)
Exon (1)1..50
Gene:HES7
Gene Synonym:
Exon (2)51..146
Gene:HES7
Gene Synonym:
Exon (3)147..234
Gene:HES7
Gene Synonym:
Exon (4)235..1667
Gene:HES7
Gene Synonym:
Translation
Position Chain Variation Link
3 3 a, g dbSNP:373443343
6 6 c, g dbSNP:779177302
10 10 c, t dbSNP:757447190
11 11 a, g dbSNP:753922605
17 17 c, t dbSNP:764426140
18 18 a, c dbSNP:370013324
22 22 a, c dbSNP:751439367
31 31 a, g dbSNP:766073916
43 43 a, g dbSNP:762996641
44 44 c, t dbSNP:772989452
60 60 a, c dbSNP:758157463
67 67 c, t dbSNP:750181156
76 76 a, g dbSNP:765170412
78 78 c, g dbSNP:761667617
81 81 c, t dbSNP:113994160
83 83 a, g dbSNP:753408923
86 86 a, c dbSNP:763576909
87 87 a, c dbSNP:372627919
96 96 a, c, t dbSNP:368880766
101 101 a, c, t dbSNP:759833400
109 109 a, g dbSNP:774790059
118 118 a, t dbSNP:371599866
119 119 c, g dbSNP:771634586
122 122 a, g dbSNP:749800673
127 127 c, t dbSNP:778047084
131 131 a, g dbSNP:61731639
138 138 a, c, t dbSNP:781290618
139 139 c, g, t dbSNP:750219149
172 172 a, g dbSNP:763593387
176 176 c, g dbSNP:773886666
180 180 a, g dbSNP:387906979
183 183 c, t dbSNP:1348325
186 186 c, g dbSNP:201434695
192 192 g, t dbSNP:368366222
198 198 g, t dbSNP:781262011
227 227 a, g dbSNP:780283648
232 232 c, g dbSNP:768825167
233 233 c, g dbSNP:747215611
239 239 a, t dbSNP:747054086
244 244 a, g dbSNP:375153301
248 248 c, t dbSNP:775714752
279 279 a, g, t dbSNP:749089405
286 286 c, t dbSNP:777620410
289 289 c, t dbSNP:756066802
299 299 a, c dbSNP:748097279
302 302 g, t dbSNP:780606563
312 312 c, t dbSNP:754563797
332 332 c, g dbSNP:751064332
341 341 c, t dbSNP:766142714
343 343 c, g dbSNP:563910734
347 347 c, t dbSNP:750815853
348 348 g, t dbSNP:765619430
351 351 a, c, g dbSNP:777228993
354 354 g, t dbSNP:764537982
369 369 g, t dbSNP:542325604
373 373 g, t dbSNP:376794942
376 376 a, g dbSNP:775485766
377 377 a, c, t dbSNP:746077060
378 378 c, g dbSNP:772931911
382 382 a, g dbSNP:769601476
387 387 a, c, t dbSNP:375056498
388 388 g, t dbSNP:201278621
391 391 a, c dbSNP:746521932
395 395 a, c, g dbSNP:56066045
397 397 c, t dbSNP:758179296
399 399 a, c dbSNP:750066544
402 402 -, aaaccgcccc dbSNP:398122970
402 402 c, g dbSNP:779182972
407 407 a, c, g dbSNP:62063067
410 410 g, t dbSNP:757665555
411 411 c, t dbSNP:754096865
412 412 c, t dbSNP:764593175
413 413 a, g dbSNP:200833034
414 414 -, g dbSNP:771146392
415 415 a, t dbSNP:752833761
417 417 g, t dbSNP:767564054
420 420 a, c dbSNP:759718984
421 421 c, g dbSNP:774662496
422 422 c, g dbSNP:771008029
429 429 c, g dbSNP:757876441
430 430 a, c dbSNP:752201429
433 433 c, g dbSNP:761682136
435 435 c, t dbSNP:776500517
437 437 a, g dbSNP:62063066
452 452 c, t dbSNP:746924914
453 453 c, g dbSNP:577019983
458 458 c, t dbSNP:771673658
465 465 g, t dbSNP:745324579
466 466 c, t dbSNP:778643488
485 485 c, t dbSNP:756796852
495 495 c, g dbSNP:754299029
508 508 c, g dbSNP:778098548
514 514 c, t dbSNP:756663030
523 523 c, g dbSNP:753173122
539 539 a, g dbSNP:767973429
550 550 a, c dbSNP:550425522
564 564 g, t dbSNP:387906978
572 572 a, c dbSNP:759681801
584 584 c, t dbSNP:558811781
655 655 c, t dbSNP:536990518
674 674 a, c dbSNP:575817248
697 697 a, g dbSNP:8076664
698 698 g, t dbSNP:535914789
721 721 c, g dbSNP:565453136
767 767 g, t dbSNP:8076629
800 800 c, t dbSNP:751740177
801 801 c, g dbSNP:766566795
802 802 c, t dbSNP:559461603
817 817 g, t dbSNP:114049508
819 819 c, t dbSNP:571199056
820 820 g, t dbSNP:552824640
821 821 a, c dbSNP:531263972
832 832 a, g dbSNP:541012018
980 980 g, t dbSNP:773751563
1057 1057 c, g dbSNP:753603130
1095 1095 a, c dbSNP:765727546
1140 1140 c, g dbSNP:74967814
1179 1179 a, g dbSNP:192195537
1192 1192 a, g dbSNP:530180639
1199 1199 c, g dbSNP:559802816
1209 1209 a, g dbSNP:188007795
1219 1219 a, g dbSNP:771638699
1242 1242 a, g dbSNP:182882481
1243 1243 a, g dbSNP:573726684
1246 1246 a, c, g dbSNP:565171042
1247 1247 a, g dbSNP:766102687
1252 1252 a, g dbSNP:543291130
1253 1253 a, g dbSNP:555500961
1256 1256 a, g dbSNP:763077546
1261 1261 c, t dbSNP:191934127
1262 1262 a, g dbSNP:554308736
1265 1265 -, a dbSNP:763560877
1272 1272 c, t dbSNP:749082436
1276 1276 -, a dbSNP:774099063
1278 1278 a, c dbSNP:187488820
1282 1282 a, g dbSNP:536050561
1285 1285 c, g dbSNP:571997712
1287 1287 -, a dbSNP:531620664
1288 1288 g, t dbSNP:182885508
1289 1289 -, g dbSNP:772471054
1290 1290 c, g, t dbSNP:150811754
1300 1300 a, g dbSNP:781112763
1302 1302 g, t dbSNP:755082518
1305 1305 a, c dbSNP:751638498
1308 1308 a, g dbSNP:766591866
1318 1318 c, t dbSNP:571205404
1322 1322 a, g dbSNP:556220964
1331 1331 c, t dbSNP:537707573
1332 1332 c, t dbSNP:758509334
1334 1334 c, t dbSNP:190639917
1337 1337 a, c, g dbSNP:530315960
1338 1338 a, g dbSNP:1442850
1339 1339 c, g, t dbSNP:187062164
1340 1340 a, c, g dbSNP:141418362
1343 1343 c, g dbSNP:565256703
1344 1344 a, c, g dbSNP:184104843
1346 1346 c, g, t dbSNP:531460779
1350 1350 a, g dbSNP:777271243
1352 1352 a, c dbSNP:540088263
1357 1357 a, t dbSNP:4792250
1364 1364 c, g dbSNP:773776763
1365 1365 a, g dbSNP:572192881
1371 1371 a, g dbSNP:75711247
1386 1386 a, c dbSNP:534725291
1421 1421 a, g dbSNP:112675209
1454 1454 a, g dbSNP:1442849
1479 1479 a, c dbSNP:547749612
1498 1498 a, g dbSNP:73243503
1510 1510 a, g dbSNP:769512869
1545 1545 a, t dbSNP:7219529
1562 1562 a, g dbSNP:748004949
1574 1574 a, c dbSNP:537845272
1582 1582 a, c dbSNP:781083843
1596 1596 a, g dbSNP:567320658

Target ORF information:

RefSeq Version NM_032580
Organism Homo sapiens (human)
Definition Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_032580

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTCACCC GGGATCGAGC TGAGAATAGG GACGGCCCCA AGATGCTCAA GCCGCTTGTG 
GAGAAGCGGC GCCGGGACCG CATCAACCGC AGCCTGGAAG AGCTGAGGCT GCTGCTGCTG
GAGCGGACCC GGGACCAGAA CCTCCGGAAC CCGAAGCTGG AGAAAGCGGA GATATTGGAG
TTCGCCGTGG GCTACTTGAG GGAGCGAAGC CGGGTGGAGC CCCCGGGGGT TCCCCGGTCC
CCAGTCCAGG ACGCCGAGGC GCTCGCCAGC TGCTACTTGT CCGGTTTCCG CGAGTGCCTG
CTTCGCTTGG CGGCCTTCGC GCACGACGCC AGCCCGGCCG CCCGCGCCCA GCTCTTCTCC
GCGCTGCACG GCTATCTGCG CCCCAAACCG CCCCGGCCCA AGCCGGTAGA TCCGAGGCCT
CCAGCGCCGC GCCCATCCCT GGACCCCGCC GCACCGGCCC TTGGCCCTGC GCTGCACCAG
CGCCCCCCAG TGCACCAGGG CCACCCTAGC CCGCGCTGCG CATGGTCCCC ATCCCTCTGC
TCCCCGCGCG CCGGGGATTC TGGCGCGCCG GCGCCCCTCA CCGGACTGCT GCCGCCGCCA
CCGCCGCCTC ACAGACAAGA CGGGGCGCCC AAGGCCCCGC TGCCCCCGCC GCCCGCTTTC
TGGAGACCTT GGCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu73665
Accession Version XM_011524038.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 798bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transcription factor HES-7 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010718.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTCGAAGG GGGCGGGGTC GGGTCCTATC CCTCCTACCC TTGGGCCGCG GAATATAAGG 
CTCCAGGCGA GCAGGGAACC GGTCCACACA GGGTCCGGAG GAGCAATGGT CACCCGGGAT
CGAGCTGAGA ATAGGGACGG CCCCAAGATG CTCAAGCCGC TTGTGGAGAA GCGGCGCCGG
GACCGCATCA ACCGCAGCCT GGAAGAGCTG AGGCTGCTGC TGCTGGAGCG GACCCGGGAC
CAGAACCTCC GGAACCCGAA GCTGGAGAAA GCGGAGATAT TGGAGTTCGC CGTGGGCTAC
TTGAGGGAGC GAAGCCGGGT GGAGCCCCCG GCCGCCGCGG CTCCAGGGGT TCCCCGGTCC
CCAGTCCAGG ACGCCGAGGC GCTCGCCAGC TGCTACTTGT CCGGTTTCCG CGAGTGCCTG
CTTCGCTTGG CGGCCTTCGC GCACGACGCC AGCCCGGCCG CCCGCGCCCA GCTCTTCTCC
GCGCTGCACG GCTATCTGCG CCCCAAACCG CCCCGGCCCA AGCCGGTAGA TCCGAGGCCT
CCAGCGCCGC GCCCATCCCT GGACCCCGCC GCACCGGCCC TTGGCCCTGC GCTGCACCAG
CGCCCCCCAG TGCACCAGGG CCACCCTAGC CCGCGCTGCG CATGGTCCCC ATCCCTCTGC
TCCCCGCGCG CCGGGGATTC TGGCGCGCCG GCGCCCCTCA CCGGACTGCT GCCGCCGCCA
CCGCCGCCTC ACAGACAAGA CGGGGCGCCC AAGGCCCCGC TGCCCCCGCC GCCCGCTTTC
TGGAGACCTT GGCCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011522340.1
CDS1071..1868
Misc Feature(1)1215..1394(+)
Misc Feature(2)1215..1340(+)
Misc Feature(3)1236..1238(+)
Misc Feature(4)1254..1394(+)
Translation
Position Chain Variation Link
6 6 a, c dbSNP:556680953
42 42 a, t dbSNP:538055974
72 72 a, g dbSNP:549058841
81 81 a, g dbSNP:527817833
135 135 a, g dbSNP:561904338
199 199 c, t dbSNP:115873433
238 238 c, t dbSNP:3027277
239 239 -, c dbSNP:563358849
241 241 a, g dbSNP:537134476
254 254 a, g dbSNP:3027278
274 274 a, c, g dbSNP:74796510
325 325 a, g dbSNP:147732388
338 338 g, t dbSNP:147109070
371 371 c, g dbSNP:764174795
411 411 c, t dbSNP:573883597
436 436 c, t dbSNP:571633632
457 457 a, g dbSNP:555571880
459 459 g, t dbSNP:186206599
500 500 a, c, g, t dbSNP:557446509
557 557 a, g dbSNP:545449025
566 566 c, t dbSNP:566870530
567 567 a, g dbSNP:551713385
570 570 c, t dbSNP:181295730
589 589 a, t dbSNP:539453867
593 593 c, t dbSNP:569281875
669 669 c, t dbSNP:754199449
707 707 a, g dbSNP:772342094
710 710 c, t dbSNP:759875050
723 723 c, t dbSNP:577725122
727 727 c, t dbSNP:771230960
739 739 a, g dbSNP:370038532
762 762 a, c dbSNP:140639313
767 767 c, t dbSNP:569780220
771 771 a, c dbSNP:778158504
780 780 c, g dbSNP:76043663
792 792 c, g dbSNP:528151922
814 814 g, t dbSNP:564033972
884 884 a, c dbSNP:541073484
924 924 c, t dbSNP:746575011
933 933 a, c dbSNP:545734619
934 934 c, t dbSNP:1372868
938 938 c, g dbSNP:563332966
956 956 c, t dbSNP:142522759
985 985 a, c dbSNP:3027279
1031 1031 c, t dbSNP:555508164
1037 1037 g, t dbSNP:534135790
1042 1042 g, t dbSNP:573244161
1062 1062 a, g dbSNP:557967918
1084 1084 c, t dbSNP:539964275
1089 1089 g, t dbSNP:548755732
1109 1109 a, c dbSNP:374740468
1122 1122 a, t dbSNP:370696397
1125 1125 a, t dbSNP:377593611
1127 1127 a, g dbSNP:764372854
1135 1135 a, g dbSNP:535717649
1137 1137 a, g dbSNP:773960448
1138 1138 a, c dbSNP:766344830
1144 1144 a, g dbSNP:3027280
1151 1151 a, g dbSNP:772835673
1152 1152 a, g dbSNP:769269700
1154 1154 a, c, t dbSNP:377276277
1157 1157 c, t dbSNP:768245361
1158 1158 a, g dbSNP:747409917
1162 1162 a, g dbSNP:530341935
1165 1165 c, t dbSNP:528275024
1170 1170 a, g dbSNP:373443343
1173 1173 c, g dbSNP:779177302
1177 1177 c, t dbSNP:757447190
1178 1178 a, g dbSNP:753922605
1184 1184 c, t dbSNP:764426140
1185 1185 a, c dbSNP:370013324
1189 1189 a, c dbSNP:751439367
1198 1198 a, g dbSNP:766073916
1210 1210 a, g dbSNP:762996641
1211 1211 c, t dbSNP:772989452
1227 1227 a, c dbSNP:758157463
1234 1234 c, t dbSNP:750181156
1243 1243 a, g dbSNP:765170412
1245 1245 c, g dbSNP:761667617
1248 1248 c, t dbSNP:113994160
1250 1250 a, g dbSNP:753408923
1253 1253 a, c dbSNP:763576909
1254 1254 a, c dbSNP:372627919
1263 1263 a, c, t dbSNP:368880766
1268 1268 a, c, t dbSNP:759833400
1276 1276 a, g dbSNP:774790059
1285 1285 a, t dbSNP:371599866
1286 1286 c, g dbSNP:771634586
1289 1289 a, g dbSNP:749800673
1294 1294 c, t dbSNP:778047084
1298 1298 a, g dbSNP:61731639
1305 1305 a, c, t dbSNP:781290618
1306 1306 c, g, t dbSNP:750219149
1339 1339 a, g dbSNP:763593387
1343 1343 c, g dbSNP:773886666
1347 1347 a, g dbSNP:387906979
1350 1350 c, t dbSNP:1348325
1353 1353 c, g dbSNP:201434695
1359 1359 g, t dbSNP:368366222
1365 1365 g, t dbSNP:781262011
1394 1394 a, g dbSNP:780283648
1399 1399 c, g dbSNP:768825167
1400 1400 c, g dbSNP:747215611
1411 1411 c, t dbSNP:768755302
1421 1421 a, t dbSNP:747054086
1426 1426 a, g dbSNP:375153301
1430 1430 c, t dbSNP:775714752
1461 1461 a, g, t dbSNP:749089405
1468 1468 c, t dbSNP:777620410
1471 1471 c, t dbSNP:756066802
1481 1481 a, c dbSNP:748097279
1484 1484 g, t dbSNP:780606563
1494 1494 c, t dbSNP:754563797
1514 1514 c, g dbSNP:751064332
1523 1523 c, t dbSNP:766142714
1525 1525 c, g dbSNP:563910734
1529 1529 c, t dbSNP:750815853
1530 1530 g, t dbSNP:765619430
1533 1533 a, c, g dbSNP:777228993
1536 1536 g, t dbSNP:764537982
1551 1551 g, t dbSNP:542325604
1555 1555 g, t dbSNP:376794942
1558 1558 a, g dbSNP:775485766
1559 1559 a, c, t dbSNP:746077060
1560 1560 c, g dbSNP:772931911
1564 1564 a, g dbSNP:769601476
1569 1569 a, c, t dbSNP:375056498
1570 1570 g, t dbSNP:201278621
1573 1573 a, c dbSNP:746521932
1577 1577 a, c, g dbSNP:56066045
1579 1579 c, t dbSNP:758179296
1581 1581 a, c dbSNP:750066544
1584 1584 -, aaaccgcccc dbSNP:398122970
1584 1584 c, g dbSNP:779182972
1589 1589 a, c, g dbSNP:62063067
1592 1592 g, t dbSNP:757665555
1593 1593 c, t dbSNP:754096865
1594 1594 c, t dbSNP:764593175
1595 1595 a, g dbSNP:200833034
1596 1596 -, g dbSNP:771146392
1597 1597 a, t dbSNP:752833761
1599 1599 g, t dbSNP:767564054
1602 1602 a, c dbSNP:759718984
1603 1603 c, g dbSNP:774662496
1604 1604 c, g dbSNP:771008029
1611 1611 c, g dbSNP:757876441
1612 1612 a, c dbSNP:752201429
1615 1615 c, g dbSNP:761682136
1617 1617 c, t dbSNP:776500517
1619 1619 a, g dbSNP:62063066
1634 1634 c, t dbSNP:746924914
1635 1635 c, g dbSNP:577019983
1640 1640 c, t dbSNP:771673658
1647 1647 g, t dbSNP:745324579
1648 1648 c, t dbSNP:778643488
1667 1667 c, t dbSNP:756796852
1677 1677 c, g dbSNP:754299029
1690 1690 c, g dbSNP:778098548
1696 1696 c, t dbSNP:756663030
1705 1705 c, g dbSNP:753173122
1721 1721 a, g dbSNP:767973429
1732 1732 a, c dbSNP:550425522
1746 1746 g, t dbSNP:387906978
1754 1754 a, c dbSNP:759681801
1766 1766 c, t dbSNP:558811781
1837 1837 c, t dbSNP:536990518
1856 1856 a, c dbSNP:575817248
1879 1879 a, g dbSNP:8076664
1880 1880 g, t dbSNP:535914789
1903 1903 c, g dbSNP:565453136
1949 1949 g, t dbSNP:8076629
1982 1982 c, t dbSNP:751740177
1983 1983 c, g dbSNP:766566795
1984 1984 c, t dbSNP:559461603
1999 1999 g, t dbSNP:114049508
2001 2001 c, t dbSNP:571199056
2002 2002 g, t dbSNP:552824640
2003 2003 a, c dbSNP:531263972
2014 2014 a, g dbSNP:541012018
2162 2162 g, t dbSNP:773751563
2239 2239 c, g dbSNP:753603130
2277 2277 a, c dbSNP:765727546
2322 2322 c, g dbSNP:74967814
2361 2361 a, g dbSNP:192195537
2374 2374 a, g dbSNP:530180639
2381 2381 c, g dbSNP:559802816
2391 2391 a, g dbSNP:188007795
2401 2401 a, g dbSNP:771638699
2424 2424 a, g dbSNP:182882481
2425 2425 a, g dbSNP:573726684
2428 2428 a, c, g dbSNP:565171042
2429 2429 a, g dbSNP:766102687
2434 2434 a, g dbSNP:543291130
2435 2435 a, g dbSNP:555500961
2438 2438 a, g dbSNP:763077546
2443 2443 c, t dbSNP:191934127
2444 2444 a, g dbSNP:554308736
2447 2447 -, a dbSNP:763560877
2454 2454 c, t dbSNP:749082436
2458 2458 -, a dbSNP:774099063
2460 2460 a, c dbSNP:187488820
2464 2464 a, g dbSNP:536050561
2467 2467 c, g dbSNP:571997712
2469 2469 -, a dbSNP:531620664
2470 2470 g, t dbSNP:182885508
2471 2471 -, g dbSNP:772471054
2472 2472 c, g, t dbSNP:150811754
2482 2482 a, g dbSNP:781112763
2484 2484 g, t dbSNP:755082518
2487 2487 a, c dbSNP:751638498
2490 2490 a, g dbSNP:766591866
2500 2500 c, t dbSNP:571205404
2504 2504 a, g dbSNP:556220964
2513 2513 c, t dbSNP:537707573
2514 2514 c, t dbSNP:758509334
2516 2516 c, t dbSNP:190639917
2519 2519 a, c, g dbSNP:530315960
2520 2520 a, g dbSNP:1442850
2521 2521 c, g, t dbSNP:187062164
2522 2522 a, c, g dbSNP:141418362
2525 2525 c, g dbSNP:565256703
2526 2526 a, c, g dbSNP:184104843
2528 2528 c, g, t dbSNP:531460779
2532 2532 a, g dbSNP:777271243
2534 2534 a, c dbSNP:540088263
2539 2539 a, t dbSNP:4792250
2546 2546 c, g dbSNP:773776763
2547 2547 a, g dbSNP:572192881
2553 2553 a, g dbSNP:75711247
2568 2568 a, c dbSNP:534725291
2603 2603 a, g dbSNP:112675209
2636 2636 a, g dbSNP:1442849
2661 2661 a, c dbSNP:547749612
2680 2680 a, g dbSNP:73243503
2692 2692 a, g dbSNP:769512869
2727 2727 a, t dbSNP:7219529
2744 2744 a, g dbSNP:748004949
2756 2756 a, c dbSNP:537845272
2764 2764 a, c dbSNP:781083843
2778 2778 a, g dbSNP:567320658

Target ORF information:

RefSeq Version XM_011524038
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524038

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTCGAAGG GGGCGGGGTC GGGTCCTATC CCTCCTACCC TTGGGCCGCG GAATATAAGG 
CTCCAGGCGA GCAGGGAACC GGTCCACACA GGGTCCGGAG GAGCAATGGT CACCCGGGAT
CGAGCTGAGA ATAGGGACGG CCCCAAGATG CTCAAGCCGC TTGTGGAGAA GCGGCGCCGG
GACCGCATCA ACCGCAGCCT GGAAGAGCTG AGGCTGCTGC TGCTGGAGCG GACCCGGGAC
CAGAACCTCC GGAACCCGAA GCTGGAGAAA GCGGAGATAT TGGAGTTCGC CGTGGGCTAC
TTGAGGGAGC GAAGCCGGGT GGAGCCCCCG GCCGCCGCGG CTCCAGGGGT TCCCCGGTCC
CCAGTCCAGG ACGCCGAGGC GCTCGCCAGC TGCTACTTGT CCGGTTTCCG CGAGTGCCTG
CTTCGCTTGG CGGCCTTCGC GCACGACGCC AGCCCGGCCG CCCGCGCCCA GCTCTTCTCC
GCGCTGCACG GCTATCTGCG CCCCAAACCG CCCCGGCCCA AGCCGGTAGA TCCGAGGCCT
CCAGCGCCGC GCCCATCCCT GGACCCCGCC GCACCGGCCC TTGGCCCTGC GCTGCACCAG
CGCCCCCCAG TGCACCAGGG CCACCCTAGC CCGCGCTGCG CATGGTCCCC ATCCCTCTGC
TCCCCGCGCG CCGGGGATTC TGGCGCGCCG GCGCCCCTCA CCGGACTGCT GCCGCCGCCA
CCGCCGCCTC ACAGACAAGA CGGGGCGCCC AAGGCCCCGC TGCCCCCGCC GCCCGCTTTC
TGGAGACCTT GGCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu73666
Accession Version XM_011524039.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 789bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transcription factor HES-7 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010718.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGCCGG CCCTAGGCAA TCTGGGGTCT TGTGGCCGGG GTGGAAGCCG GGCTCTCCCA 
GCGGCGGGAT CCGACGCCGG GGCGGCCGCA CTGAGTCTCG GTCTGGTCGG CCCGCTCTCC
TTTTCTCGCT GGTCGCAGAT GCTCAAGCCG CTTGTGGAGA AGCGGCGCCG GGACCGCATC
AACCGCAGCC TGGAAGAGCT GAGGCTGCTG CTGCTGGAGC GGACCCGGGA CCAGAACCTC
CGGAACCCGA AGCTGGAGAA AGCGGAGATA TTGGAGTTCG CCGTGGGCTA CTTGAGGGAG
CGAAGCCGGG TGGAGCCCCC GGCCGCCGCG GCTCCAGGGG TTCCCCGGTC CCCAGTCCAG
GACGCCGAGG CGCTCGCCAG CTGCTACTTG TCCGGTTTCC GCGAGTGCCT GCTTCGCTTG
GCGGCCTTCG CGCACGACGC CAGCCCGGCC GCCCGCGCCC AGCTCTTCTC CGCGCTGCAC
GGCTATCTGC GCCCCAAACC GCCCCGGCCC AAGCCGGTAG ATCCGAGGCC TCCAGCGCCG
CGCCCATCCC TGGACCCCGC CGCACCGGCC CTTGGCCCTG CGCTGCACCA GCGCCCCCCA
GTGCACCAGG GCCACCCTAG CCCGCGCTGC GCATGGTCCC CATCCCTCTG CTCCCCGCGC
GCCGGGGATT CTGGCGCGCC GGCGCCCCTC ACCGGACTGC TGCCGCCGCC ACCGCCGCCT
CACAGACAAG ACGGGGCGCC CAAGGCCCCG CTGCCCCCGC CGCCCGCTTT CTGGAGACCT
TGGCCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011522341.1
CDS1939..2727
Misc Feature(1)2083..2253(+)
Misc Feature(2)2092..2199(+)
Misc Feature(3)2095..2097(+)
Misc Feature(4)2113..2253(+)
Translation
Position Chain Variation Link
1 1 g, t dbSNP:533106625
2 2 a, g dbSNP:148082379
3 3 a, g dbSNP:111723640
4 4 a, g dbSNP:73972696
5 5 a, c, g dbSNP:191299746
6 6 a, g dbSNP:143663718
7 7 g, t dbSNP:540375930
8 8 c, t dbSNP:775700443
9 9 c, t dbSNP:372468302
13 13 a, g dbSNP:552198572
19 19 c, t dbSNP:3027275
46 46 -, cggacc dbSNP:371204554
47 47 g, t dbSNP:557501163
51 51 -, ccggac dbSNP:113463257
89 89 a, g dbSNP:746300343
113 113 a, g dbSNP:528351779
135 135 a, g dbSNP:779279104
143 143 c, t dbSNP:539296079
151 151 c, g dbSNP:561205471
188 188 a, c dbSNP:556680953
224 224 a, t dbSNP:538055974
270 270 g, t dbSNP:747580486
280 280 c, g dbSNP:780713568
281 281 c, t dbSNP:567372104
300 300 c, g dbSNP:754559560
301 301 c, g dbSNP:751201065
403 403 a, g dbSNP:549058841
412 412 a, g dbSNP:527817833
451 451 c, t dbSNP:566838815
476 476 g, t dbSNP:551507162
477 477 g, t dbSNP:533222100
486 486 c, t dbSNP:779918733
495 495 g, t dbSNP:189536656
502 502 a, c dbSNP:138805696
543 543 a, g dbSNP:3027276
615 615 a, g dbSNP:561904338
679 679 c, t dbSNP:115873433
718 718 c, t dbSNP:3027277
719 719 -, c dbSNP:563358849
721 721 a, g dbSNP:537134476
734 734 a, g dbSNP:3027278
754 754 a, c, g dbSNP:74796510
805 805 a, g dbSNP:147732388
818 818 g, t dbSNP:147109070
851 851 c, g dbSNP:764174795
891 891 c, t dbSNP:573883597
916 916 c, t dbSNP:571633632
937 937 a, g dbSNP:555571880
939 939 g, t dbSNP:186206599
980 980 a, c, g, t dbSNP:557446509
1037 1037 a, g dbSNP:545449025
1046 1046 c, t dbSNP:566870530
1047 1047 a, g dbSNP:551713385
1050 1050 c, t dbSNP:181295730
1069 1069 a, t dbSNP:539453867
1073 1073 c, t dbSNP:569281875
1149 1149 c, t dbSNP:754199449
1187 1187 a, g dbSNP:772342094
1190 1190 c, t dbSNP:759875050
1203 1203 c, t dbSNP:577725122
1207 1207 c, t dbSNP:771230960
1219 1219 a, g dbSNP:370038532
1242 1242 a, c dbSNP:140639313
1247 1247 c, t dbSNP:569780220
1251 1251 a, c dbSNP:778158504
1260 1260 c, g dbSNP:76043663
1272 1272 c, g dbSNP:528151922
1294 1294 g, t dbSNP:564033972
1364 1364 a, c dbSNP:541073484
1404 1404 c, t dbSNP:746575011
1413 1413 a, c dbSNP:545734619
1414 1414 c, t dbSNP:1372868
1418 1418 c, g dbSNP:563332966
1436 1436 c, t dbSNP:142522759
1465 1465 a, c dbSNP:3027279
1511 1511 c, t dbSNP:555508164
1517 1517 g, t dbSNP:534135790
1522 1522 g, t dbSNP:573244161
1542 1542 a, g dbSNP:557967918
1564 1564 c, t dbSNP:539964275
1569 1569 g, t dbSNP:548755732
1589 1589 a, c dbSNP:374740468
1602 1602 a, t dbSNP:370696397
1605 1605 a, t dbSNP:377593611
1607 1607 a, g dbSNP:764372854
1615 1615 a, g dbSNP:535717649
1617 1617 a, g dbSNP:773960448
1618 1618 a, c dbSNP:766344830
1624 1624 a, g dbSNP:3027280
1631 1631 a, g dbSNP:772835673
1632 1632 a, g dbSNP:769269700
1634 1634 a, c, t dbSNP:377276277
1637 1637 c, t dbSNP:768245361
1638 1638 a, g dbSNP:747409917
1642 1642 a, g dbSNP:530341935
1645 1645 c, t dbSNP:528275024
1650 1650 a, g dbSNP:373443343
1653 1653 c, g dbSNP:779177302
1657 1657 c, t dbSNP:757447190
1658 1658 a, g dbSNP:753922605
1664 1664 c, t dbSNP:764426140
1665 1665 a, c dbSNP:370013324
1669 1669 a, c dbSNP:751439367
1678 1678 a, g dbSNP:766073916
1690 1690 a, g dbSNP:762996641
1691 1691 c, t dbSNP:772989452
1716 1716 c, t dbSNP:540970072
1819 1819 -, gag dbSNP:538689772
1827 1827 a, c dbSNP:374255567
1857 1857 c, g dbSNP:772297813
1862 1862 c, g dbSNP:573348922
1873 1873 g, t dbSNP:773754529
1918 1918 c, g dbSNP:376704207
1925 1925 a, c dbSNP:370580036
1934 1934 a, g dbSNP:575713548
1944 1944 g, t dbSNP:3027281
1945 1945 c, t dbSNP:557357308
1954 1954 a, g dbSNP:535857171
1964 1964 a, g dbSNP:568312773
1965 1965 g, t dbSNP:143317942
1988 1988 c, g dbSNP:760984393
2020 2020 c, g dbSNP:77130240
2037 2037 c, g dbSNP:534956126
2040 2040 g, t dbSNP:749382799
2063 2063 c, t dbSNP:777996899
2067 2067 c, t dbSNP:756416284
2070 2070 -, g dbSNP:775539610
2073 2073 a, g, t dbSNP:766234398
2076 2076 -, cgcag dbSNP:771978237
2086 2086 a, c dbSNP:758157463
2093 2093 c, t dbSNP:750181156
2102 2102 a, g dbSNP:765170412
2104 2104 c, g dbSNP:761667617
2107 2107 c, t dbSNP:113994160
2109 2109 a, g dbSNP:753408923
2112 2112 a, c dbSNP:763576909
2113 2113 a, c dbSNP:372627919
2122 2122 a, c, t dbSNP:368880766
2127 2127 a, c, t dbSNP:759833400
2135 2135 a, g dbSNP:774790059
2144 2144 a, t dbSNP:371599866
2145 2145 c, g dbSNP:771634586
2148 2148 a, g dbSNP:749800673
2153 2153 c, t dbSNP:778047084
2157 2157 a, g dbSNP:61731639
2164 2164 a, c, t dbSNP:781290618
2165 2165 c, g, t dbSNP:750219149
2198 2198 a, g dbSNP:763593387
2202 2202 c, g dbSNP:773886666
2206 2206 a, g dbSNP:387906979
2209 2209 c, t dbSNP:1348325
2212 2212 c, g dbSNP:201434695
2218 2218 g, t dbSNP:368366222
2224 2224 g, t dbSNP:781262011
2253 2253 a, g dbSNP:780283648
2258 2258 c, g dbSNP:768825167
2259 2259 c, g dbSNP:747215611
2270 2270 c, t dbSNP:768755302
2280 2280 a, t dbSNP:747054086
2285 2285 a, g dbSNP:375153301
2289 2289 c, t dbSNP:775714752
2320 2320 a, g, t dbSNP:749089405
2327 2327 c, t dbSNP:777620410
2330 2330 c, t dbSNP:756066802
2340 2340 a, c dbSNP:748097279
2343 2343 g, t dbSNP:780606563
2353 2353 c, t dbSNP:754563797
2373 2373 c, g dbSNP:751064332
2382 2382 c, t dbSNP:766142714
2384 2384 c, g dbSNP:563910734
2388 2388 c, t dbSNP:750815853
2389 2389 g, t dbSNP:765619430
2392 2392 a, c, g dbSNP:777228993
2395 2395 g, t dbSNP:764537982
2410 2410 g, t dbSNP:542325604
2414 2414 g, t dbSNP:376794942
2417 2417 a, g dbSNP:775485766
2418 2418 a, c, t dbSNP:746077060
2419 2419 c, g dbSNP:772931911
2423 2423 a, g dbSNP:769601476
2428 2428 a, c, t dbSNP:375056498
2429 2429 g, t dbSNP:201278621
2432 2432 a, c dbSNP:746521932
2436 2436 a, c, g dbSNP:56066045
2438 2438 c, t dbSNP:758179296
2440 2440 a, c dbSNP:750066544
2443 2443 -, aaaccgcccc dbSNP:398122970
2443 2443 c, g dbSNP:779182972
2448 2448 a, c, g dbSNP:62063067
2451 2451 g, t dbSNP:757665555
2452 2452 c, t dbSNP:754096865
2453 2453 c, t dbSNP:764593175
2454 2454 a, g dbSNP:200833034
2455 2455 -, g dbSNP:771146392
2456 2456 a, t dbSNP:752833761
2458 2458 g, t dbSNP:767564054
2461 2461 a, c dbSNP:759718984
2462 2462 c, g dbSNP:774662496
2463 2463 c, g dbSNP:771008029
2470 2470 c, g dbSNP:757876441
2471 2471 a, c dbSNP:752201429
2474 2474 c, g dbSNP:761682136
2476 2476 c, t dbSNP:776500517
2478 2478 a, g dbSNP:62063066
2493 2493 c, t dbSNP:746924914
2494 2494 c, g dbSNP:577019983
2499 2499 c, t dbSNP:771673658
2506 2506 g, t dbSNP:745324579
2507 2507 c, t dbSNP:778643488
2526 2526 c, t dbSNP:756796852
2536 2536 c, g dbSNP:754299029
2549 2549 c, g dbSNP:778098548
2555 2555 c, t dbSNP:756663030
2564 2564 c, g dbSNP:753173122
2580 2580 a, g dbSNP:767973429
2591 2591 a, c dbSNP:550425522
2605 2605 g, t dbSNP:387906978
2613 2613 a, c dbSNP:759681801
2625 2625 c, t dbSNP:558811781
2696 2696 c, t dbSNP:536990518
2715 2715 a, c dbSNP:575817248
2738 2738 a, g dbSNP:8076664
2739 2739 g, t dbSNP:535914789
2762 2762 c, g dbSNP:565453136
2808 2808 g, t dbSNP:8076629
2841 2841 c, t dbSNP:751740177
2842 2842 c, g dbSNP:766566795
2843 2843 c, t dbSNP:559461603
2858 2858 g, t dbSNP:114049508
2860 2860 c, t dbSNP:571199056
2861 2861 g, t dbSNP:552824640
2862 2862 a, c dbSNP:531263972
2873 2873 a, g dbSNP:541012018
3021 3021 g, t dbSNP:773751563
3098 3098 c, g dbSNP:753603130
3136 3136 a, c dbSNP:765727546
3181 3181 c, g dbSNP:74967814
3220 3220 a, g dbSNP:192195537
3233 3233 a, g dbSNP:530180639
3240 3240 c, g dbSNP:559802816
3250 3250 a, g dbSNP:188007795
3260 3260 a, g dbSNP:771638699
3283 3283 a, g dbSNP:182882481
3284 3284 a, g dbSNP:573726684
3287 3287 a, c, g dbSNP:565171042
3288 3288 a, g dbSNP:766102687
3293 3293 a, g dbSNP:543291130
3294 3294 a, g dbSNP:555500961
3297 3297 a, g dbSNP:763077546
3302 3302 c, t dbSNP:191934127
3303 3303 a, g dbSNP:554308736
3306 3306 -, a dbSNP:763560877
3313 3313 c, t dbSNP:749082436
3317 3317 -, a dbSNP:774099063
3319 3319 a, c dbSNP:187488820

Target ORF information:

RefSeq Version XM_011524039
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524039

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGCCGG CCCTAGGCAA TCTGGGGTCT TGTGGCCGGG GTGGAAGCCG GGCTCTCCCA 
GCGGCGGGAT CCGACGCCGG GGCGGCCGCA CTGAGTCTCG GTCTGGTCGG CCCGCTCTCC
TTTTCTCGCT GGTCGCAGAT GCTCAAGCCG CTTGTGGAGA AGCGGCGCCG GGACCGCATC
AACCGCAGCC TGGAAGAGCT GAGGCTGCTG CTGCTGGAGC GGACCCGGGA CCAGAACCTC
CGGAACCCGA AGCTGGAGAA AGCGGAGATA TTGGAGTTCG CCGTGGGCTA CTTGAGGGAG
CGAAGCCGGG TGGAGCCCCC GGCCGCCGCG GCTCCAGGGG TTCCCCGGTC CCCAGTCCAG
GACGCCGAGG CGCTCGCCAG CTGCTACTTG TCCGGTTTCC GCGAGTGCCT GCTTCGCTTG
GCGGCCTTCG CGCACGACGC CAGCCCGGCC GCCCGCGCCC AGCTCTTCTC CGCGCTGCAC
GGCTATCTGC GCCCCAAACC GCCCCGGCCC AAGCCGGTAG ATCCGAGGCC TCCAGCGCCG
CGCCCATCCC TGGACCCCGC CGCACCGGCC CTTGGCCCTG CGCTGCACCA GCGCCCCCCA
GTGCACCAGG GCCACCCTAG CCCGCGCTGC GCATGGTCCC CATCCCTCTG CTCCCCGCGC
GCCGGGGATT CTGGCGCGCC GGCGCCCCTC ACCGGACTGC TGCCGCCGCC ACCGCCGCCT
CACAGACAAG ACGGGGCGCC CAAGGCCCCG CTGCCCCCGC CGCCCGCTTT CTGGAGACCT
TGGCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu73666
Accession Version XM_011524040.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 789bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transcription factor HES-7 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010718.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGCCGG CCCTAGGCAA TCTGGGGTCT TGTGGCCGGG GTGGAAGCCG GGCTCTCCCA 
GCGGCGGGAT CCGACGCCGG GGCGGCCGCA CTGAGTCTCG GTCTGGTCGG CCCGCTCTCC
TTTTCTCGCT GGTCGCAGAT GCTCAAGCCG CTTGTGGAGA AGCGGCGCCG GGACCGCATC
AACCGCAGCC TGGAAGAGCT GAGGCTGCTG CTGCTGGAGC GGACCCGGGA CCAGAACCTC
CGGAACCCGA AGCTGGAGAA AGCGGAGATA TTGGAGTTCG CCGTGGGCTA CTTGAGGGAG
CGAAGCCGGG TGGAGCCCCC GGCCGCCGCG GCTCCAGGGG TTCCCCGGTC CCCAGTCCAG
GACGCCGAGG CGCTCGCCAG CTGCTACTTG TCCGGTTTCC GCGAGTGCCT GCTTCGCTTG
GCGGCCTTCG CGCACGACGC CAGCCCGGCC GCCCGCGCCC AGCTCTTCTC CGCGCTGCAC
GGCTATCTGC GCCCCAAACC GCCCCGGCCC AAGCCGGTAG ATCCGAGGCC TCCAGCGCCG
CGCCCATCCC TGGACCCCGC CGCACCGGCC CTTGGCCCTG CGCTGCACCA GCGCCCCCCA
GTGCACCAGG GCCACCCTAG CCCGCGCTGC GCATGGTCCC CATCCCTCTG CTCCCCGCGC
GCCGGGGATT CTGGCGCGCC GGCGCCCCTC ACCGGACTGC TGCCGCCGCC ACCGCCGCCT
CACAGACAAG ACGGGGCGCC CAAGGCCCCG CTGCCCCCGC CGCCCGCTTT CTGGAGACCT
TGGCCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011522342.1
CDS1562..2350
Misc Feature(1)1706..1876(+)
Misc Feature(2)1715..1822(+)
Misc Feature(3)1718..1720(+)
Misc Feature(4)1736..1876(+)
Translation
Position Chain Variation Link
1 1 g, t dbSNP:533106625
2 2 a, g dbSNP:148082379
3 3 a, g dbSNP:111723640
4 4 a, g dbSNP:73972696
5 5 a, c, g dbSNP:191299746
6 6 a, g dbSNP:143663718
7 7 g, t dbSNP:540375930
8 8 c, t dbSNP:775700443
9 9 c, t dbSNP:372468302
13 13 a, g dbSNP:552198572
19 19 c, t dbSNP:3027275
46 46 -, cggacc dbSNP:371204554
47 47 g, t dbSNP:557501163
51 51 -, ccggac dbSNP:113463257
89 89 a, g dbSNP:746300343
113 113 a, g dbSNP:528351779
135 135 a, g dbSNP:779279104
143 143 c, t dbSNP:539296079
151 151 c, g dbSNP:561205471
188 188 a, c dbSNP:556680953
224 224 a, t dbSNP:538055974
270 270 g, t dbSNP:747580486
280 280 c, g dbSNP:780713568
281 281 c, t dbSNP:567372104
300 300 c, g dbSNP:754559560
301 301 c, g dbSNP:751201065
403 403 a, g dbSNP:549058841
412 412 a, g dbSNP:527817833
451 451 c, t dbSNP:566838815
476 476 g, t dbSNP:551507162
477 477 g, t dbSNP:533222100
486 486 c, t dbSNP:779918733
495 495 g, t dbSNP:189536656
502 502 a, c dbSNP:138805696
543 543 a, g dbSNP:3027276
615 615 a, g dbSNP:561904338
679 679 c, t dbSNP:115873433
718 718 c, t dbSNP:3027277
719 719 -, c dbSNP:563358849
721 721 a, g dbSNP:537134476
734 734 a, g dbSNP:3027278
754 754 a, c, g dbSNP:74796510
805 805 a, g dbSNP:147732388
818 818 g, t dbSNP:147109070
851 851 c, g dbSNP:764174795
891 891 c, t dbSNP:573883597
916 916 c, t dbSNP:571633632
937 937 a, g dbSNP:555571880
939 939 g, t dbSNP:186206599
980 980 a, c, g, t dbSNP:557446509
1037 1037 a, g dbSNP:545449025
1046 1046 c, t dbSNP:566870530
1047 1047 a, g dbSNP:551713385
1050 1050 c, t dbSNP:181295730
1069 1069 a, t dbSNP:539453867
1073 1073 c, t dbSNP:569281875
1149 1149 c, t dbSNP:754199449
1187 1187 a, g dbSNP:772342094
1190 1190 c, t dbSNP:759875050
1203 1203 c, t dbSNP:577725122
1207 1207 c, t dbSNP:771230960
1219 1219 a, g dbSNP:370038532
1242 1242 a, c dbSNP:140639313
1247 1247 c, t dbSNP:569780220
1251 1251 a, c dbSNP:778158504
1260 1260 c, g dbSNP:76043663
1265 1265 a, g dbSNP:530341935
1268 1268 c, t dbSNP:528275024
1273 1273 a, g dbSNP:373443343
1276 1276 c, g dbSNP:779177302
1280 1280 c, t dbSNP:757447190
1281 1281 a, g dbSNP:753922605
1287 1287 c, t dbSNP:764426140
1288 1288 a, c dbSNP:370013324
1292 1292 a, c dbSNP:751439367
1301 1301 a, g dbSNP:766073916
1313 1313 a, g dbSNP:762996641
1314 1314 c, t dbSNP:772989452
1339 1339 c, t dbSNP:540970072
1442 1442 -, gag dbSNP:538689772
1450 1450 a, c dbSNP:374255567
1480 1480 c, g dbSNP:772297813
1485 1485 c, g dbSNP:573348922
1496 1496 g, t dbSNP:773754529
1541 1541 c, g dbSNP:376704207
1548 1548 a, c dbSNP:370580036
1557 1557 a, g dbSNP:575713548
1567 1567 g, t dbSNP:3027281
1568 1568 c, t dbSNP:557357308
1577 1577 a, g dbSNP:535857171
1587 1587 a, g dbSNP:568312773
1588 1588 g, t dbSNP:143317942
1611 1611 c, g dbSNP:760984393
1643 1643 c, g dbSNP:77130240
1660 1660 c, g dbSNP:534956126
1663 1663 g, t dbSNP:749382799
1686 1686 c, t dbSNP:777996899
1690 1690 c, t dbSNP:756416284
1693 1693 -, g dbSNP:775539610
1696 1696 a, g, t dbSNP:766234398
1699 1699 -, cgcag dbSNP:771978237
1709 1709 a, c dbSNP:758157463
1716 1716 c, t dbSNP:750181156
1725 1725 a, g dbSNP:765170412
1727 1727 c, g dbSNP:761667617
1730 1730 c, t dbSNP:113994160
1732 1732 a, g dbSNP:753408923
1735 1735 a, c dbSNP:763576909
1736 1736 a, c dbSNP:372627919
1745 1745 a, c, t dbSNP:368880766
1750 1750 a, c, t dbSNP:759833400
1758 1758 a, g dbSNP:774790059
1767 1767 a, t dbSNP:371599866
1768 1768 c, g dbSNP:771634586
1771 1771 a, g dbSNP:749800673
1776 1776 c, t dbSNP:778047084
1780 1780 a, g dbSNP:61731639
1787 1787 a, c, t dbSNP:781290618
1788 1788 c, g, t dbSNP:750219149
1821 1821 a, g dbSNP:763593387
1825 1825 c, g dbSNP:773886666
1829 1829 a, g dbSNP:387906979
1832 1832 c, t dbSNP:1348325
1835 1835 c, g dbSNP:201434695
1841 1841 g, t dbSNP:368366222
1847 1847 g, t dbSNP:781262011
1876 1876 a, g dbSNP:780283648
1881 1881 c, g dbSNP:768825167
1882 1882 c, g dbSNP:747215611
1893 1893 c, t dbSNP:768755302
1903 1903 a, t dbSNP:747054086
1908 1908 a, g dbSNP:375153301
1912 1912 c, t dbSNP:775714752
1943 1943 a, g, t dbSNP:749089405
1950 1950 c, t dbSNP:777620410
1953 1953 c, t dbSNP:756066802
1963 1963 a, c dbSNP:748097279
1966 1966 g, t dbSNP:780606563
1976 1976 c, t dbSNP:754563797
1996 1996 c, g dbSNP:751064332
2005 2005 c, t dbSNP:766142714
2007 2007 c, g dbSNP:563910734
2011 2011 c, t dbSNP:750815853
2012 2012 g, t dbSNP:765619430
2015 2015 a, c, g dbSNP:777228993
2018 2018 g, t dbSNP:764537982
2033 2033 g, t dbSNP:542325604
2037 2037 g, t dbSNP:376794942
2040 2040 a, g dbSNP:775485766
2041 2041 a, c, t dbSNP:746077060
2042 2042 c, g dbSNP:772931911
2046 2046 a, g dbSNP:769601476
2051 2051 a, c, t dbSNP:375056498
2052 2052 g, t dbSNP:201278621
2055 2055 a, c dbSNP:746521932
2059 2059 a, c, g dbSNP:56066045
2061 2061 c, t dbSNP:758179296
2063 2063 a, c dbSNP:750066544
2066 2066 -, aaaccgcccc dbSNP:398122970
2066 2066 c, g dbSNP:779182972
2071 2071 a, c, g dbSNP:62063067
2074 2074 g, t dbSNP:757665555
2075 2075 c, t dbSNP:754096865
2076 2076 c, t dbSNP:764593175
2077 2077 a, g dbSNP:200833034
2078 2078 -, g dbSNP:771146392
2079 2079 a, t dbSNP:752833761
2081 2081 g, t dbSNP:767564054
2084 2084 a, c dbSNP:759718984
2085 2085 c, g dbSNP:774662496
2086 2086 c, g dbSNP:771008029
2093 2093 c, g dbSNP:757876441
2094 2094 a, c dbSNP:752201429
2097 2097 c, g dbSNP:761682136
2099 2099 c, t dbSNP:776500517
2101 2101 a, g dbSNP:62063066
2116 2116 c, t dbSNP:746924914
2117 2117 c, g dbSNP:577019983
2122 2122 c, t dbSNP:771673658
2129 2129 g, t dbSNP:745324579
2130 2130 c, t dbSNP:778643488
2149 2149 c, t dbSNP:756796852
2159 2159 c, g dbSNP:754299029
2172 2172 c, g dbSNP:778098548
2178 2178 c, t dbSNP:756663030
2187 2187 c, g dbSNP:753173122
2203 2203 a, g dbSNP:767973429
2214 2214 a, c dbSNP:550425522
2228 2228 g, t dbSNP:387906978
2236 2236 a, c dbSNP:759681801
2248 2248 c, t dbSNP:558811781
2319 2319 c, t dbSNP:536990518
2338 2338 a, c dbSNP:575817248
2361 2361 a, g dbSNP:8076664
2362 2362 g, t dbSNP:535914789
2385 2385 c, g dbSNP:565453136
2431 2431 g, t dbSNP:8076629
2464 2464 c, t dbSNP:751740177
2465 2465 c, g dbSNP:766566795
2466 2466 c, t dbSNP:559461603
2481 2481 g, t dbSNP:114049508
2483 2483 c, t dbSNP:571199056
2484 2484 g, t dbSNP:552824640
2485 2485 a, c dbSNP:531263972
2496 2496 a, g dbSNP:541012018
2644 2644 g, t dbSNP:773751563
2721 2721 c, g dbSNP:753603130
2759 2759 a, c dbSNP:765727546
2804 2804 c, g dbSNP:74967814
2843 2843 a, g dbSNP:192195537
2856 2856 a, g dbSNP:530180639
2863 2863 c, g dbSNP:559802816
2873 2873 a, g dbSNP:188007795
2883 2883 a, g dbSNP:771638699
2906 2906 a, g dbSNP:182882481
2907 2907 a, g dbSNP:573726684
2910 2910 a, c, g dbSNP:565171042
2911 2911 a, g dbSNP:766102687
2916 2916 a, g dbSNP:543291130
2917 2917 a, g dbSNP:555500961
2920 2920 a, g dbSNP:763077546
2925 2925 c, t dbSNP:191934127
2926 2926 a, g dbSNP:554308736
2929 2929 -, a dbSNP:763560877
2936 2936 c, t dbSNP:749082436
2940 2940 -, a dbSNP:774099063
2942 2942 a, c dbSNP:187488820

Target ORF information:

RefSeq Version XM_011524040
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524040

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGGCCGG CCCTAGGCAA TCTGGGGTCT TGTGGCCGGG GTGGAAGCCG GGCTCTCCCA 
GCGGCGGGAT CCGACGCCGG GGCGGCCGCA CTGAGTCTCG GTCTGGTCGG CCCGCTCTCC
TTTTCTCGCT GGTCGCAGAT GCTCAAGCCG CTTGTGGAGA AGCGGCGCCG GGACCGCATC
AACCGCAGCC TGGAAGAGCT GAGGCTGCTG CTGCTGGAGC GGACCCGGGA CCAGAACCTC
CGGAACCCGA AGCTGGAGAA AGCGGAGATA TTGGAGTTCG CCGTGGGCTA CTTGAGGGAG
CGAAGCCGGG TGGAGCCCCC GGCCGCCGCG GCTCCAGGGG TTCCCCGGTC CCCAGTCCAG
GACGCCGAGG CGCTCGCCAG CTGCTACTTG TCCGGTTTCC GCGAGTGCCT GCTTCGCTTG
GCGGCCTTCG CGCACGACGC CAGCCCGGCC GCCCGCGCCC AGCTCTTCTC CGCGCTGCAC
GGCTATCTGC GCCCCAAACC GCCCCGGCCC AAGCCGGTAG ATCCGAGGCC TCCAGCGCCG
CGCCCATCCC TGGACCCCGC CGCACCGGCC CTTGGCCCTG CGCTGCACCA GCGCCCCCCA
GTGCACCAGG GCCACCCTAG CCCGCGCTGC GCATGGTCCC CATCCCTCTG CTCCCCGCGC
GCCGGGGATT CTGGCGCGCC GGCGCCCCTC ACCGGACTGC TGCCGCCGCC ACCGCCGCCT
CACAGACAAG ACGGGGCGCC CAAGGCCCCG CTGCCCCCGC CGCCCGCTTT CTGGAGACCT
TGGCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu73667
Accession Version XM_011524041.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 780bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transcription factor HES-7 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010718.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCCAGGG GCGGCCCCAC ACCCGGGTGC GAACAGCCGG GGGCCCCCAG CCTCGCGTGC 
AGACACTTTG TCCTGAAGCC CTCGAGTGGT CAGGCATCGT TCTGCGGAGG GTCATGCTCT
TGGTTTGAGA TGCTCAAGCC GCTTGTGGAG AAGCGGCGCC GGGACCGCAT CAACCGCAGC
CTGGAAGAGC TGAGGCTGCT GCTGCTGGAG CGGACCCGGG ACCAGAACCT CCGGAACCCG
AAGCTGGAGA AAGCGGAGAT ATTGGAGTTC GCCGTGGGCT ACTTGAGGGA GCGAAGCCGG
GTGGAGCCCC CGGCCGCCGC GGCTCCAGGG GTTCCCCGGT CCCCAGTCCA GGACGCCGAG
GCGCTCGCCA GCTGCTACTT GTCCGGTTTC CGCGAGTGCC TGCTTCGCTT GGCGGCCTTC
GCGCACGACG CCAGCCCGGC CGCCCGCGCC CAGCTCTTCT CCGCGCTGCA CGGCTATCTG
CGCCCCAAAC CGCCCCGGCC CAAGCCGGTA GATCCGAGGC CTCCAGCGCC GCGCCCATCC
CTGGACCCCG CCGCACCGGC CCTTGGCCCT GCGCTGCACC AGCGCCCCCC AGTGCACCAG
GGCCACCCTA GCCCGCGCTG CGCATGGTCC CCATCCCTCT GCTCCCCGCG CGCCGGGGAT
TCTGGCGCGC CGGCGCCCCT CACCGGACTG CTGCCGCCGC CACCGCCGCC TCACAGACAA
GACGGGGCGC CCAAGGCCCC GCTGCCCCCG CCGCCCGCTT TCTGGAGACC TTGGCCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011522343.1
CDS167..946
Misc Feature(1)302..472(+)
Misc Feature(2)311..418(+)
Misc Feature(3)314..316(+)
Misc Feature(4)332..472(+)
Translation
Position Chain Variation Link
1 1 g, t dbSNP:533106625
2 2 a, g dbSNP:148082379
3 3 a, g dbSNP:111723640
4 4 a, g dbSNP:73972696
5 5 a, c, g dbSNP:191299746
6 6 a, g dbSNP:143663718
7 7 g, t dbSNP:540375930
8 8 c, t dbSNP:775700443
9 9 c, t dbSNP:372468302
13 13 a, g dbSNP:552198572
19 19 c, t dbSNP:3027275
46 46 -, cggacc dbSNP:371204554
47 47 g, t dbSNP:557501163
51 51 -, ccggac dbSNP:113463257
89 89 a, g dbSNP:746300343
113 113 a, g dbSNP:528351779
135 135 a, g dbSNP:779279104
143 143 c, t dbSNP:539296079
151 151 c, g dbSNP:561205471
188 188 a, c dbSNP:556680953
224 224 a, t dbSNP:538055974
258 258 a, g dbSNP:561904338
305 305 a, c dbSNP:758157463
312 312 c, t dbSNP:750181156
321 321 a, g dbSNP:765170412
323 323 c, g dbSNP:761667617
326 326 c, t dbSNP:113994160
328 328 a, g dbSNP:753408923
331 331 a, c dbSNP:763576909
332 332 a, c dbSNP:372627919
341 341 a, c, t dbSNP:368880766
346 346 a, c, t dbSNP:759833400
354 354 a, g dbSNP:774790059
363 363 a, t dbSNP:371599866
364 364 c, g dbSNP:771634586
367 367 a, g dbSNP:749800673
372 372 c, t dbSNP:778047084
376 376 a, g dbSNP:61731639
383 383 a, c, t dbSNP:781290618
384 384 c, g, t dbSNP:750219149
417 417 a, g dbSNP:763593387
421 421 c, g dbSNP:773886666
425 425 a, g dbSNP:387906979
428 428 c, t dbSNP:1348325
431 431 c, g dbSNP:201434695
437 437 g, t dbSNP:368366222
443 443 g, t dbSNP:781262011
472 472 a, g dbSNP:780283648
477 477 c, g dbSNP:768825167
478 478 c, g dbSNP:747215611
489 489 c, t dbSNP:768755302
499 499 a, t dbSNP:747054086
504 504 a, g dbSNP:375153301
508 508 c, t dbSNP:775714752
539 539 a, g, t dbSNP:749089405
546 546 c, t dbSNP:777620410
549 549 c, t dbSNP:756066802
559 559 a, c dbSNP:748097279
562 562 g, t dbSNP:780606563
572 572 c, t dbSNP:754563797
592 592 c, g dbSNP:751064332
601 601 c, t dbSNP:766142714
603 603 c, g dbSNP:563910734
607 607 c, t dbSNP:750815853
608 608 g, t dbSNP:765619430
611 611 a, c, g dbSNP:777228993
614 614 g, t dbSNP:764537982
629 629 g, t dbSNP:542325604
633 633 g, t dbSNP:376794942
636 636 a, g dbSNP:775485766
637 637 a, c, t dbSNP:746077060
638 638 c, g dbSNP:772931911
642 642 a, g dbSNP:769601476
647 647 a, c, t dbSNP:375056498
648 648 g, t dbSNP:201278621
651 651 a, c dbSNP:746521932
655 655 a, c, g dbSNP:56066045
657 657 c, t dbSNP:758179296
659 659 a, c dbSNP:750066544
662 662 -, aaaccgcccc dbSNP:398122970
662 662 c, g dbSNP:779182972
667 667 a, c, g dbSNP:62063067
670 670 g, t dbSNP:757665555
671 671 c, t dbSNP:754096865
672 672 c, t dbSNP:764593175
673 673 a, g dbSNP:200833034
674 674 -, g dbSNP:771146392
675 675 a, t dbSNP:752833761
677 677 g, t dbSNP:767564054
680 680 a, c dbSNP:759718984
681 681 c, g dbSNP:774662496
682 682 c, g dbSNP:771008029
689 689 c, g dbSNP:757876441
690 690 a, c dbSNP:752201429
693 693 c, g dbSNP:761682136
695 695 c, t dbSNP:776500517
697 697 a, g dbSNP:62063066
712 712 c, t dbSNP:746924914
713 713 c, g dbSNP:577019983
718 718 c, t dbSNP:771673658
725 725 g, t dbSNP:745324579
726 726 c, t dbSNP:778643488
745 745 c, t dbSNP:756796852
755 755 c, g dbSNP:754299029
768 768 c, g dbSNP:778098548
774 774 c, t dbSNP:756663030
783 783 c, g dbSNP:753173122
799 799 a, g dbSNP:767973429
810 810 a, c dbSNP:550425522
824 824 g, t dbSNP:387906978
832 832 a, c dbSNP:759681801
844 844 c, t dbSNP:558811781
915 915 c, t dbSNP:536990518
934 934 a, c dbSNP:575817248
957 957 a, g dbSNP:8076664
958 958 g, t dbSNP:535914789
981 981 c, g dbSNP:565453136
1027 1027 g, t dbSNP:8076629
1060 1060 c, t dbSNP:751740177
1061 1061 c, g dbSNP:766566795
1062 1062 c, t dbSNP:559461603
1077 1077 g, t dbSNP:114049508
1079 1079 c, t dbSNP:571199056
1080 1080 g, t dbSNP:552824640
1081 1081 a, c dbSNP:531263972
1092 1092 a, g dbSNP:541012018
1240 1240 g, t dbSNP:773751563
1317 1317 c, g dbSNP:753603130
1355 1355 a, c dbSNP:765727546
1400 1400 c, g dbSNP:74967814
1439 1439 a, g dbSNP:192195537
1452 1452 a, g dbSNP:530180639
1459 1459 c, g dbSNP:559802816
1469 1469 a, g dbSNP:188007795
1479 1479 a, g dbSNP:771638699
1502 1502 a, g dbSNP:182882481
1503 1503 a, g dbSNP:573726684
1506 1506 a, c, g dbSNP:565171042
1507 1507 a, g dbSNP:766102687
1512 1512 a, g dbSNP:543291130
1513 1513 a, g dbSNP:555500961
1516 1516 a, g dbSNP:763077546
1521 1521 c, t dbSNP:191934127
1522 1522 a, g dbSNP:554308736
1525 1525 -, a dbSNP:763560877
1532 1532 c, t dbSNP:749082436
1536 1536 -, a dbSNP:774099063
1538 1538 a, c dbSNP:187488820

Target ORF information:

RefSeq Version XM_011524041
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524041

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCCAGGG GCGGCCCCAC ACCCGGGTGC GAACAGCCGG GGGCCCCCAG CCTCGCGTGC 
AGACACTTTG TCCTGAAGCC CTCGAGTGGT CAGGCATCGT TCTGCGGAGG GTCATGCTCT
TGGTTTGAGA TGCTCAAGCC GCTTGTGGAG AAGCGGCGCC GGGACCGCAT CAACCGCAGC
CTGGAAGAGC TGAGGCTGCT GCTGCTGGAG CGGACCCGGG ACCAGAACCT CCGGAACCCG
AAGCTGGAGA AAGCGGAGAT ATTGGAGTTC GCCGTGGGCT ACTTGAGGGA GCGAAGCCGG
GTGGAGCCCC CGGCCGCCGC GGCTCCAGGG GTTCCCCGGT CCCCAGTCCA GGACGCCGAG
GCGCTCGCCA GCTGCTACTT GTCCGGTTTC CGCGAGTGCC TGCTTCGCTT GGCGGCCTTC
GCGCACGACG CCAGCCCGGC CGCCCGCGCC CAGCTCTTCT CCGCGCTGCA CGGCTATCTG
CGCCCCAAAC CGCCCCGGCC CAAGCCGGTA GATCCGAGGC CTCCAGCGCC GCGCCCATCC
CTGGACCCCG CCGCACCGGC CCTTGGCCCT GCGCTGCACC AGCGCCCCCC AGTGCACCAG
GGCCACCCTA GCCCGCGCTG CGCATGGTCC CCATCCCTCT GCTCCCCGCG CGCCGGGGAT
TCTGGCGCGC CGGCGCCCCT CACCGGACTG CTGCCGCCGC CACCGCCGCC TCACAGACAA
GACGGGGCGC CCAAGGCCCC GCTGCCCCCG CCGCCCGCTT TCTGGAGACC TTGGCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu73668
Accession Version XM_011524042.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 651bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transcription factor HES-7 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010718.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGCTCAAGC CGCTTGTGGA GAAGCGGCGC CGGGACCGCA TCAACCGCAG CCTGGAAGAG 
CTGAGGCTGC TGCTGCTGGA GCGGACCCGG GACCAGAACC TCCGGAACCC GAAGCTGGAG
AAAGCGGAGA TATTGGAGTT CGCCGTGGGC TACTTGAGGG AGCGAAGCCG GGTGGAGCCC
CCGGCCGCCG CGGCTCCAGG GGTTCCCCGG TCCCCAGTCC AGGACGCCGA GGCGCTCGCC
AGCTGCTACT TGTCCGGTTT CCGCGAGTGC CTGCTTCGCT TGGCGGCCTT CGCGCACGAC
GCCAGCCCGG CCGCCCGCGC CCAGCTCTTC TCCGCGCTGC ACGGCTATCT GCGCCCCAAA
CCGCCCCGGC CCAAGCCGGT AGATCCGAGG CCTCCAGCGC CGCGCCCATC CCTGGACCCC
GCCGCACCGG CCCTTGGCCC TGCGCTGCAC CAGCGCCCCC CAGTGCACCA GGGCCACCCT
AGCCCGCGCT GCGCATGGTC CCCATCCCTC TGCTCCCCGC GCGCCGGGGA TTCTGGCGCG
CCGGCGCCCC TCACCGGACT GCTGCCGCCG CCACCGCCGC CTCACAGACA AGACGGGGCG
CCCAAGGCCC CGCTGCCCCC GCCGCCCGCT TTCTGGAGAC CTTGGCCCTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011522344.1
CDS508..1158
Misc Feature(1)514..684(+)
Misc Feature(2)523..630(+)
Misc Feature(3)526..528(+)
Misc Feature(4)544..684(+)
Translation
Position Chain Variation Link
4 4 a, g dbSNP:765117482
6 6 a, g dbSNP:761332203
8 8 a, g dbSNP:776060105
25 25 c, t dbSNP:768298203
35 35 c, t dbSNP:1372869
36 36 a, g dbSNP:551974812
47 47 c, t dbSNP:764087675
57 57 c, g dbSNP:188931517
59 59 c, t dbSNP:184488032
135 135 a, g dbSNP:760733427
226 226 g, t dbSNP:541441476
233 233 c, t dbSNP:752827618
235 235 c, t dbSNP:192142938
253 253 a, g dbSNP:759764944
279 279 a, g dbSNP:774459527
310 310 a, g dbSNP:74600532
442 442 a, g dbSNP:765131994
458 458 a, g dbSNP:763234051
477 477 -, t dbSNP:759354489
517 517 a, c dbSNP:758157463
524 524 c, t dbSNP:750181156
533 533 a, g dbSNP:765170412
535 535 c, g dbSNP:761667617
538 538 c, t dbSNP:113994160
540 540 a, g dbSNP:753408923
543 543 a, c dbSNP:763576909
544 544 a, c dbSNP:372627919
553 553 a, c, t dbSNP:368880766
558 558 a, c, t dbSNP:759833400
566 566 a, g dbSNP:774790059
575 575 a, t dbSNP:371599866
576 576 c, g dbSNP:771634586
579 579 a, g dbSNP:749800673
584 584 c, t dbSNP:778047084
588 588 a, g dbSNP:61731639
595 595 a, c, t dbSNP:781290618
596 596 c, g, t dbSNP:750219149
629 629 a, g dbSNP:763593387
633 633 c, g dbSNP:773886666
637 637 a, g dbSNP:387906979
640 640 c, t dbSNP:1348325
643 643 c, g dbSNP:201434695
649 649 g, t dbSNP:368366222
655 655 g, t dbSNP:781262011
684 684 a, g dbSNP:780283648
689 689 c, g dbSNP:768825167
690 690 c, g dbSNP:747215611
701 701 c, t dbSNP:768755302
711 711 a, t dbSNP:747054086
716 716 a, g dbSNP:375153301
720 720 c, t dbSNP:775714752
751 751 a, g, t dbSNP:749089405
758 758 c, t dbSNP:777620410
761 761 c, t dbSNP:756066802
771 771 a, c dbSNP:748097279
774 774 g, t dbSNP:780606563
784 784 c, t dbSNP:754563797
804 804 c, g dbSNP:751064332
813 813 c, t dbSNP:766142714
815 815 c, g dbSNP:563910734
819 819 c, t dbSNP:750815853
820 820 g, t dbSNP:765619430
823 823 a, c, g dbSNP:777228993
826 826 g, t dbSNP:764537982
841 841 g, t dbSNP:542325604
845 845 g, t dbSNP:376794942
848 848 a, g dbSNP:775485766
849 849 a, c, t dbSNP:746077060
850 850 c, g dbSNP:772931911
854 854 a, g dbSNP:769601476
859 859 a, c, t dbSNP:375056498
860 860 g, t dbSNP:201278621
863 863 a, c dbSNP:746521932
867 867 a, c, g dbSNP:56066045
869 869 c, t dbSNP:758179296
871 871 a, c dbSNP:750066544
874 874 -, aaaccgcccc dbSNP:398122970
874 874 c, g dbSNP:779182972
879 879 a, c, g dbSNP:62063067
882 882 g, t dbSNP:757665555
883 883 c, t dbSNP:754096865
884 884 c, t dbSNP:764593175
885 885 a, g dbSNP:200833034
886 886 -, g dbSNP:771146392
887 887 a, t dbSNP:752833761
889 889 g, t dbSNP:767564054
892 892 a, c dbSNP:759718984
893 893 c, g dbSNP:774662496
894 894 c, g dbSNP:771008029
901 901 c, g dbSNP:757876441
902 902 a, c dbSNP:752201429
905 905 c, g dbSNP:761682136
907 907 c, t dbSNP:776500517
909 909 a, g dbSNP:62063066
924 924 c, t dbSNP:746924914
925 925 c, g dbSNP:577019983
930 930 c, t dbSNP:771673658
937 937 g, t dbSNP:745324579
938 938 c, t dbSNP:778643488
957 957 c, t dbSNP:756796852
967 967 c, g dbSNP:754299029
980 980 c, g dbSNP:778098548
986 986 c, t dbSNP:756663030
995 995 c, g dbSNP:753173122
1011 1011 a, g dbSNP:767973429
1022 1022 a, c dbSNP:550425522
1036 1036 g, t dbSNP:387906978
1044 1044 a, c dbSNP:759681801
1056 1056 c, t dbSNP:558811781
1127 1127 c, t dbSNP:536990518
1146 1146 a, c dbSNP:575817248
1169 1169 a, g dbSNP:8076664
1170 1170 g, t dbSNP:535914789
1193 1193 c, g dbSNP:565453136
1239 1239 g, t dbSNP:8076629
1272 1272 c, t dbSNP:751740177
1273 1273 c, g dbSNP:766566795
1274 1274 c, t dbSNP:559461603
1289 1289 g, t dbSNP:114049508
1291 1291 c, t dbSNP:571199056
1292 1292 g, t dbSNP:552824640
1293 1293 a, c dbSNP:531263972
1304 1304 a, g dbSNP:541012018
1452 1452 g, t dbSNP:773751563
1529 1529 c, g dbSNP:753603130
1567 1567 a, c dbSNP:765727546
1612 1612 c, g dbSNP:74967814
1651 1651 a, g dbSNP:192195537
1664 1664 a, g dbSNP:530180639
1671 1671 c, g dbSNP:559802816
1681 1681 a, g dbSNP:188007795
1691 1691 a, g dbSNP:771638699
1714 1714 a, g dbSNP:182882481
1715 1715 a, g dbSNP:573726684
1718 1718 a, c, g dbSNP:565171042
1719 1719 a, g dbSNP:766102687
1724 1724 a, g dbSNP:543291130
1725 1725 a, g dbSNP:555500961
1728 1728 a, g dbSNP:763077546
1733 1733 c, t dbSNP:191934127
1734 1734 a, g dbSNP:554308736
1737 1737 -, a dbSNP:763560877
1744 1744 c, t dbSNP:749082436
1748 1748 -, a dbSNP:774099063
1750 1750 a, c dbSNP:187488820

Target ORF information:

RefSeq Version XM_011524042
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524042

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGCTCAAGC CGCTTGTGGA GAAGCGGCGC CGGGACCGCA TCAACCGCAG CCTGGAAGAG 
CTGAGGCTGC TGCTGCTGGA GCGGACCCGG GACCAGAACC TCCGGAACCC GAAGCTGGAG
AAAGCGGAGA TATTGGAGTT CGCCGTGGGC TACTTGAGGG AGCGAAGCCG GGTGGAGCCC
CCGGCCGCCG CGGCTCCAGG GGTTCCCCGG TCCCCAGTCC AGGACGCCGA GGCGCTCGCC
AGCTGCTACT TGTCCGGTTT CCGCGAGTGC CTGCTTCGCT TGGCGGCCTT CGCGCACGAC
GCCAGCCCGG CCGCCCGCGC CCAGCTCTTC TCCGCGCTGC ACGGCTATCT GCGCCCCAAA
CCGCCCCGGC CCAAGCCGGT AGATCCGAGG CCTCCAGCGC CGCGCCCATC CCTGGACCCC
GCCGCACCGG CCCTTGGCCC TGCGCTGCAC CAGCGCCCCC CAGTGCACCA GGGCCACCCT
AGCCCGCGCT GCGCATGGTC CCCATCCCTC TGCTCCCCGC GCGCCGGGGA TTCTGGCGCG
CCGGCGCCCC TCACCGGACT GCTGCCGCCG CCACCGCCGC CTCACAGACA AGACGGGGCG
CCCAAGGCCC CGCTGCCCCC GCCGCCCGCT TTCTGGAGAC CTTGGCCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22574
Accession Version NM_001165967.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 693bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product transcription factor HES-7 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI259590.1, BC039887.1 and AC129492.6. Summary: This gene encodes a member of the hairy and enhancer of split family of bHLH transcription factors. The mouse ortholog of this gene is regulated by Notch signaling. The protein functions as a transcriptional repressor, and is implicated in correct patterning of the axial skeleton. A mutation in this gene has been shown to result in spondylocostal dysostosis. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC039887.1, BI335370.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTCACCC GGGATCGAGC TGAGAATAGG GACGGCCCCA AGATGCTCAA GCCGCTTGTG 
GAGAAGCGGC GCCGGGACCG CATCAACCGC AGCCTGGAAG AGCTGAGGCT GCTGCTGCTG
GAGCGGACCC GGGACCAGAA CCTCCGGAAC CCGAAGCTGG AGAAAGCGGA GATATTGGAG
TTCGCCGTGG GCTACTTGAG GGAGCGAAGC CGGGTGGAGC CCCCGGCCGC CGCGGCTCCA
GGGGTTCCCC GGTCCCCAGT CCAGGACGCC GAGGCGCTCG CCAGCTGCTA CTTGTCCGGT
TTCCGCGAGT GCCTGCTTCG CTTGGCGGCC TTCGCGCACG ACGCCAGCCC GGCCGCCCGC
GCCCAGCTCT TCTCCGCGCT GCACGGCTAT CTGCGCCCCA AACCGCCCCG GCCCAAGCCG
GTAGATCCGA GGCCTCCAGC GCCGCGCCCA TCCCTGGACC CCGCCGCACC GGCCCTTGGC
CCTGCGCTGC ACCAGCGCCC CCCAGTGCAC CAGGGCCACC CTAGCCCGCG CTGCGCATGG
TCCCCATCCC TCTGCTCCCC GCGCGCCGGG GATTCTGGCG CGCCGGCGCC CCTCACCGGA
CTGCTGCCGC CGCCACCGCC GCCTCACAGA CAAGACGGGG CGCCCAAGGC CCCGCTGCCC
CCGCCGCCCG CTTTCTGGAG ACCTTGGCCC TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001159439.1
CDS9..701
Misc Feature(1)48..227(+)
Misc Feature(2)48..173(+)
Misc Feature(3)69..71(+)
Misc Feature(4)87..227(+)
Exon (1)1..50
Gene:HES7
Gene Synonym:
Exon (2)51..146
Gene:HES7
Gene Synonym:
Exon (3)147..234
Gene:HES7
Gene Synonym:
Exon (4)235..1682
Gene:HES7
Gene Synonym:
Translation
Position Chain Variation Link
3 3 a, g dbSNP:373443343
6 6 c, g dbSNP:779177302
10 10 c, t dbSNP:757447190
11 11 a, g dbSNP:753922605
17 17 c, t dbSNP:764426140
18 18 a, c dbSNP:370013324
22 22 a, c dbSNP:751439367
31 31 a, g dbSNP:766073916
43 43 a, g dbSNP:762996641
44 44 c, t dbSNP:772989452
60 60 a, c dbSNP:758157463
67 67 c, t dbSNP:750181156
76 76 a, g dbSNP:765170412
78 78 c, g dbSNP:761667617
81 81 c, t dbSNP:113994160
83 83 a, g dbSNP:753408923
86 86 a, c dbSNP:763576909
87 87 a, c dbSNP:372627919
96 96 a, c, t dbSNP:368880766
101 101 a, c, t dbSNP:759833400
109 109 a, g dbSNP:774790059
118 118 a, t dbSNP:371599866
119 119 c, g dbSNP:771634586
122 122 a, g dbSNP:749800673
127 127 c, t dbSNP:778047084
131 131 a, g dbSNP:61731639
138 138 a, c, t dbSNP:781290618
139 139 c, g, t dbSNP:750219149
172 172 a, g dbSNP:763593387
176 176 c, g dbSNP:773886666
180 180 a, g dbSNP:387906979
183 183 c, t dbSNP:1348325
186 186 c, g dbSNP:201434695
192 192 g, t dbSNP:368366222
198 198 g, t dbSNP:781262011
227 227 a, g dbSNP:780283648
232 232 c, g dbSNP:768825167
233 233 c, g dbSNP:747215611
244 244 c, t dbSNP:768755302
254 254 a, t dbSNP:747054086
259 259 a, g dbSNP:375153301
263 263 c, t dbSNP:775714752
294 294 a, g, t dbSNP:749089405
301 301 c, t dbSNP:777620410
304 304 c, t dbSNP:756066802
314 314 a, c dbSNP:748097279
317 317 g, t dbSNP:780606563
327 327 c, t dbSNP:754563797
347 347 c, g dbSNP:751064332
356 356 c, t dbSNP:766142714
358 358 c, g dbSNP:563910734
362 362 c, t dbSNP:750815853
363 363 g, t dbSNP:765619430
366 366 a, c, g dbSNP:777228993
369 369 g, t dbSNP:764537982
384 384 g, t dbSNP:542325604
388 388 g, t dbSNP:376794942
391 391 a, g dbSNP:775485766
392 392 a, c, t dbSNP:746077060
393 393 c, g dbSNP:772931911
397 397 a, g dbSNP:769601476
402 402 a, c, t dbSNP:375056498
403 403 g, t dbSNP:201278621
406 406 a, c dbSNP:746521932
410 410 a, c, g dbSNP:56066045
412 412 c, t dbSNP:758179296
414 414 a, c dbSNP:750066544
417 417 -, aaaccgcccc dbSNP:398122970
417 417 c, g dbSNP:779182972
422 422 a, c, g dbSNP:62063067
425 425 g, t dbSNP:757665555
426 426 c, t dbSNP:754096865
427 427 c, t dbSNP:764593175
428 428 a, g dbSNP:200833034
429 429 -, g dbSNP:771146392
430 430 a, t dbSNP:752833761
432 432 g, t dbSNP:767564054
435 435 a, c dbSNP:759718984
436 436 c, g dbSNP:774662496
437 437 c, g dbSNP:771008029
444 444 c, g dbSNP:757876441
445 445 a, c dbSNP:752201429
448 448 c, g dbSNP:761682136
450 450 c, t dbSNP:776500517
452 452 a, g dbSNP:62063066
467 467 c, t dbSNP:746924914
468 468 c, g dbSNP:577019983
473 473 c, t dbSNP:771673658
480 480 g, t dbSNP:745324579
481 481 c, t dbSNP:778643488
500 500 c, t dbSNP:756796852
510 510 c, g dbSNP:754299029
523 523 c, g dbSNP:778098548
529 529 c, t dbSNP:756663030
538 538 c, g dbSNP:753173122
554 554 a, g dbSNP:767973429
565 565 a, c dbSNP:550425522
579 579 g, t dbSNP:387906978
587 587 a, c dbSNP:759681801
599 599 c, t dbSNP:558811781
670 670 c, t dbSNP:536990518
689 689 a, c dbSNP:575817248
712 712 a, g dbSNP:8076664
713 713 g, t dbSNP:535914789
736 736 c, g dbSNP:565453136
782 782 g, t dbSNP:8076629
815 815 c, t dbSNP:751740177
816 816 c, g dbSNP:766566795
817 817 c, t dbSNP:559461603
832 832 g, t dbSNP:114049508
834 834 c, t dbSNP:571199056
835 835 g, t dbSNP:552824640
836 836 a, c dbSNP:531263972
847 847 a, g dbSNP:541012018
995 995 g, t dbSNP:773751563
1072 1072 c, g dbSNP:753603130
1110 1110 a, c dbSNP:765727546
1155 1155 c, g dbSNP:74967814
1194 1194 a, g dbSNP:192195537
1207 1207 a, g dbSNP:530180639
1214 1214 c, g dbSNP:559802816
1224 1224 a, g dbSNP:188007795
1234 1234 a, g dbSNP:771638699
1257 1257 a, g dbSNP:182882481
1258 1258 a, g dbSNP:573726684
1261 1261 a, c, g dbSNP:565171042
1262 1262 a, g dbSNP:766102687
1267 1267 a, g dbSNP:543291130
1268 1268 a, g dbSNP:555500961
1271 1271 a, g dbSNP:763077546
1276 1276 c, t dbSNP:191934127
1277 1277 a, g dbSNP:554308736
1280 1280 -, a dbSNP:763560877
1287 1287 c, t dbSNP:749082436
1291 1291 -, a dbSNP:774099063
1293 1293 a, c dbSNP:187488820
1297 1297 a, g dbSNP:536050561
1300 1300 c, g dbSNP:571997712
1302 1302 -, a dbSNP:531620664
1303 1303 g, t dbSNP:182885508
1304 1304 -, g dbSNP:772471054
1305 1305 c, g, t dbSNP:150811754
1315 1315 a, g dbSNP:781112763
1317 1317 g, t dbSNP:755082518
1320 1320 a, c dbSNP:751638498
1323 1323 a, g dbSNP:766591866
1333 1333 c, t dbSNP:571205404
1337 1337 a, g dbSNP:556220964
1346 1346 c, t dbSNP:537707573
1347 1347 c, t dbSNP:758509334
1349 1349 c, t dbSNP:190639917
1352 1352 a, c, g dbSNP:530315960
1353 1353 a, g dbSNP:1442850
1354 1354 c, g, t dbSNP:187062164
1355 1355 a, c, g dbSNP:141418362
1358 1358 c, g dbSNP:565256703
1359 1359 a, c, g dbSNP:184104843
1361 1361 c, g, t dbSNP:531460779
1365 1365 a, g dbSNP:777271243
1367 1367 a, c dbSNP:540088263
1372 1372 a, t dbSNP:4792250
1379 1379 c, g dbSNP:773776763
1380 1380 a, g dbSNP:572192881
1386 1386 a, g dbSNP:75711247
1401 1401 a, c dbSNP:534725291
1436 1436 a, g dbSNP:112675209
1469 1469 a, g dbSNP:1442849
1494 1494 a, c dbSNP:547749612
1513 1513 a, g dbSNP:73243503
1525 1525 a, g dbSNP:769512869
1560 1560 a, t dbSNP:7219529
1577 1577 a, g dbSNP:748004949
1589 1589 a, c dbSNP:537845272
1597 1597 a, c dbSNP:781083843
1611 1611 a, g dbSNP:567320658

Target ORF information:

RefSeq Version NM_001165967
Organism Homo sapiens (human)
Definition Homo sapiens hes family bHLH transcription factor 7 (HES7), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001165967

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTCACCC GGGATCGAGC TGAGAATAGG GACGGCCCCA AGATGCTCAA GCCGCTTGTG 
GAGAAGCGGC GCCGGGACCG CATCAACCGC AGCCTGGAAG AGCTGAGGCT GCTGCTGCTG
GAGCGGACCC GGGACCAGAA CCTCCGGAAC CCGAAGCTGG AGAAAGCGGA GATATTGGAG
TTCGCCGTGG GCTACTTGAG GGAGCGAAGC CGGGTGGAGC CCCCGGCCGC CGCGGCTCCA
GGGGTTCCCC GGTCCCCAGT CCAGGACGCC GAGGCGCTCG CCAGCTGCTA CTTGTCCGGT
TTCCGCGAGT GCCTGCTTCG CTTGGCGGCC TTCGCGCACG ACGCCAGCCC GGCCGCCCGC
GCCCAGCTCT TCTCCGCGCT GCACGGCTAT CTGCGCCCCA AACCGCCCCG GCCCAAGCCG
GTAGATCCGA GGCCTCCAGC GCCGCGCCCA TCCCTGGACC CCGCCGCACC GGCCCTTGGC
CCTGCGCTGC ACCAGCGCCC CCCAGTGCAC CAGGGCCACC CTAGCCCGCG CTGCGCATGG
TCCCCATCCC TCTGCTCCCC GCGCGCCGGG GATTCTGGCG CGCCGGCGCC CCTCACCGGA
CTGCTGCCGC CGCCACCGCC GCCTCACAGA CAAGACGGGG CGCCCAAGGC CCCGCTGCCC
CCGCCGCCCG CTTTCTGGAG ACCTTGGCCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.