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PIGO cDNA ORF clone, Homo sapiens (human)

Gene Symbol PIGO
Entrez Gene ID 84720
Full Name phosphatidylinositol glycan anchor biosynthesis, class O
Synonyms HPMRS2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

9

9p13.3

Summary This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This protein is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI. At least three alternatively spliced transcripts encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011]. lac of sum
Disorder MIM:

614730


mRNA and Protein(s)

mRNA Protein Name
XM_005251619 XP_005251676 GPI ethanolamine phosphate transferase 3 isoform X1
XM_011518056 XP_011516358 GPI ethanolamine phosphate transferase 3 isoform X1
NM_032634 NP_116023 GPI ethanolamine phosphate transferase 3 isoform 1
NM_152850 NP_690577 GPI ethanolamine phosphate transferase 3 isoform 2
NM_001201484 NP_001188413 GPI ethanolamine phosphate transferase 3 isoform 2



Homo sapiens (human) PIGO NP_116023.2
Pan troglodytes (chimpanzee) PIGO XP_001165498.1
Macaca mulatta (Rhesus monkey) PIGO XP_001091501.1
Canis lupus familiaris (dog) PIGO XP_005626807.1
Bos taurus (cattle) PIGO XP_002689720.1
Mus musculus (house mouse) Pigo NP_064419.2
Rattus norvegicus (Norway rat) Pigo XP_233141.7
Gallus gallus (chicken) PIGO XP_004937176.1
Danio rerio (zebrafish) pigo XP_692445.3
Drosophila melanogaster (fruit fly) CG12263 NP_611332.1
Caenorhabditis elegans C27A12.9 NP_491750.3
Xenopus (Silurana) tropicalis (western clawed frog) pigo NP_001120383.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PIGO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIGO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19171 XM_005251619 PREDICTED: Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu19171 XM_011518056 PREDICTED: Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu19171 NM_032634 Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu15672
NM_152850 Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu15672
NM_001201484 Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19171
Clone ID Related Accession (Same CDS sequence) NM_032634 , XM_005251619 , XM_011518056
Accession Version XM_005251619.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product GPI ethanolamine phosphate transferase 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530390223. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGCAGAAAG CCTCAGTGTT GCTCTTCCTG GCCTGGGTCT GCTTCCTCTT CTACGCTGGC 
ATTGCCCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTTGG AGCTCACCAA CCATAGCAGC
TGCCAAGAGC CCCCAGGCCC TGGGTCCCTG CCATGGGGGA GCCAAGGGAA ACCTGGGGCC
TGCTGGATGG CTTCCCGATT TTCGCGGGTT GTGTTGGTGC TGATAGATGC TCTGCGATTT
GACTTCGCCC AGCCCCAGCA TTCACACGTG CCTAGAGAGC CTCCTGTCTC CCTACCCTTC
CTGGGCAAAC TAAGCTCCTT GCAGAGGATC CTGGAGATTC AGCCCCACCA TGCCCGGCTC
TACCGATCTC AGGTTGACCC TCCTACCACC ACCATGCAGC GCCTCAAGGC CCTCACCACT
GGCTCACTGC CTACCTTTAT TGATGCTGGT AGTAACTTCG CCAGCCACGC CATAGTGGAA
GACAATCTCA TTAAGCAGCT CACCAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT
ACCTGGAAAG ACCTTTTCCC TGGTGCTTTC TCCAAAGCTT TCTTCTTCCC ATCCTTCAAT
GTCAGAGACC TAGACACAGT GGACAATGGC ATCCTGGAAC ACCTCTACCC CACCATGGAC
AGTGGTGAAT GGGACGTGCT GATTGCTCAC TTCCTGGGTG TGGACCACTG TGGCCACAAG
CATGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATCCAG
GGACTTGTGG AGCGTCTGGA GAATGACACA CTGCTGGTAG TGGCTGGGGA CCATGGGATG
ACCACAAATG GAGACCATGG AGGGGACAGT GAGCTGGAGG TCTCAGCTGC TCTCTTTCTG
TATAGCCCCA CAGCAGTCTT CCCCAGCACC CCACCAGAGG AGCCAGAGGT GATTCCTCAA
GTTAGCCTTG TGCCCACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGGAATATC
GGGGAAGTGA TGGCTGAGCT ATTCTCAGGG GGTGAGGACT CCCAGCCCCA CTCCTCTGCT
TTAGCCCAAG CCTCAGCTCT CCATCTCAAT GCTCAGCAGG TGTCCCGATT TCTTCATACC
TACTCAGCTG CTACTCAGGA CCTTCAAGCT AAGGAGCTTC ATCAGCTGCA GAACCTCTTC
TCCAAGGCCT CTGCTGACTA CCAGTGGCTT CTCCAGAGCC CCAAGGGGGC TGAGGCGACA
CTGCCGACTG TGATTGCTGA GCTGCAGCAG TTCCTGCGGG GAGCTCGGGC CATGTGCATC
GAGTCTTGGG CTCGTTTCTC TCTGGTCCGC ATGGCGGGGG GTACTGCTCT CTTGGCTGCT
TCCTGCTTTA TCTGCCTGCT GGCATCTCAG TGGGCAATAT CCCCAGGCTT TCCATTCTGC
CCTCTACTCC TGACACCTGT GGCCTGGGGC CTGGTTGGGG CCATAGCGTA TGCTGGACTC
CTGGGAACTA TTGAGCTGAA GCTAGATCTA GTGCTTCTAG GGGCTGTGGC TGCAGTGAGC
TCATTCCTCC CTTTTCTGTG GAAAGCCTGG GCTGGCTGGG GGTCCAAGAG GCCCCTGGCA
ACCCTGTTTC CCATCCCTGG GCCCGTCCTG TTACTCCTGC TGTTTCGCTT GGCTGTGTTC
TTCTCTGATA GTTTTGTTGT AGCTGAGGCC AGGGCCACCC CCTTCCTTTT GGGCTCATTC
ATCCTGCTCC TGGTTGTCCA GCTTCACTGG GAGGGCCAGC TGCTTCCACC TAAGCTACTC
ACAATGCCCC GCCTTGGCAC TTCAGCCACA ACAAACCCCC CACGGCACAA TGGTGCATAT
GCCCTGAGGC TTGGAATTGG GTTGCTTTTA TGTACAAGGC TAGCTGGGCT TTTTCATCGT
TGCCCTGAAG AGACACCTGT TTGCCACTCC TCTCCCTGGC TGAGTCCTCT GGCATCCATG
GTGGGTGGTC GAGCCAAGAA TTTGTGGTAT GGAGCTTGTG TGGCGGCGCT GGTGGCCCTG
TTAGCTGCCG TGCGCTTGTG GCTTCGCCGC TATGGTAATC TCAAGAGCCC CGAGCCACCC
ATGCTCTTTG TGCGCTGGGG ACTGCCCCTA ATGGCATTGG GTACTGCTGC CTACTGGGCA
TTGGCGTCGG GGGCAGATGA GGCTCCCCCC CGTCTCCGGG TCCTGGTCTC TGGGGCATCC
ATGGTGCTGC CTCGGGCTGT AGCAGGGCTG GCTGCTTCAG GGCTCGCGCT GCTGCTCTGG
AAGCCTGTGA CAGTGCTGGT GAAGGCTGGG GCAGGCGCTC CAAGGACCAG GACTGTCCTC
ACTCCCTTCT CAGGCCCCCC CACTTCTCAA GCTGACTTGG ATTATGTGGT CCCTCAAATC
TACCGACACA TGCAGGAGGA GTTCCGGGGC CGGTTAGAGA GGACCAAATC TCAGGGTCCC
CTGACTGTGG CTGCTTATCA GTTGGGGAGT GTCTACTCAG CTGCTATGGT CACAGCCCTC
ACCCTGTTGG CCTTCCCACT TCTGCTGTTG CATGCGGAGC GCATCAGCCT TGTGTTCCTG
CTTCTGTTTC TGCAGAGCTT CCTTCTCCTA CATCTGCTTG CTGCTGGGAT ACCCGTCACC
ACCCCTGGTC CTTTTACTGT GCCATGGCAG GCAGTCTCGG CTTGGGCCCT CATGGCCACA
CAGACCTTCT ACTCCACAGG CCACCAGCCT GTCTTTCCAG CCATCCATTG GCATGCAGCC
TTCGTGGGAT TCCCAGAGGG TCATGGCTCC TGTACTTGGC TGCCTGCTTT GCTAGTGGGA
GCCAACACCT TTGCCTCCCA CCTCCTCTTT GCAGTAGGTT GCCCACTGCT CCTGCTCTGG
CCTTTCCTGT GTGAGAGTCA AGGGCTGCGG AAGAGACAGC AGCCCCCAGG GAATGAAGCT
GATGCCAGAG TCAGACCCGA GGAGGAAGAG GAGCCACTGA TGGAGATGCG GCTCCGGGAT
GCGCCTCAGC ACTTCTATGC AGCACTGCTG CAGCTGGGCC TCAAGTACCT CTTTATCCTT
GGTATTCAGA TTCTGGCCTG TGCCTTGGCA GCCTCCATCC TTCGCAGGCA TCTCATGGTC
TGGAAAGTGT TTGCCCCTAA GTTCATATTT GAGGCTGTGG GCTTCATTGT GAGCAGCGTG
GGACTTCTCC TGGGCATAGC TTTGGTGATG AGAGTGGATG GTGCTGTGAG CTCCTGGTTC
AGGCAGCTAT TTCTGGCCCA GCAGAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005251676.1
CDS22..3291
Misc Feature(1)145..>918(+)
Misc Feature(2)229..>879(+)
Translation

Target ORF information:

RefSeq Version XM_005251619
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005251619

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGCAGAAAG CCTCAGTGTT GCTCTTCCTG GCCTGGGTCT GCTTCCTCTT CTACGCTGGC 
ATTGCCCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTTGG AGCTCACCAA CCATAGCAGC
TGCCAAGAGC CCCCAGGCCC TGGGTCCCTG CCATGGGGGA GCCAAGGGAA ACCTGGGGCC
TGCTGGATGG CTTCCCGATT TTCGCGGGTT GTGTTGGTGC TGATAGATGC TCTGCGATTT
GACTTCGCCC AGCCCCAGCA TTCACACGTG CCTAGAGAGC CTCCTGTCTC CCTACCCTTC
CTGGGCAAAC TAAGCTCCTT GCAGAGGATC CTGGAGATTC AGCCCCACCA TGCCCGGCTC
TACCGATCTC AGGTTGACCC TCCTACCACC ACCATGCAGC GCCTCAAGGC CCTCACCACT
GGCTCACTGC CTACCTTTAT TGATGCTGGT AGTAACTTCG CCAGCCACGC CATAGTGGAA
GACAATCTCA TTAAGCAGCT CACCAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT
ACCTGGAAAG ACCTTTTCCC TGGTGCTTTC TCCAAAGCTT TCTTCTTCCC ATCCTTCAAT
GTCAGAGACC TAGACACAGT GGACAATGGC ATCCTGGAAC ACCTCTACCC CACCATGGAC
AGTGGTGAAT GGGACGTGCT GATTGCTCAC TTCCTGGGTG TGGACCACTG TGGCCACAAG
CATGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATCCAG
GGACTTGTGG AGCGTCTGGA GAATGACACA CTGCTGGTAG TGGCTGGGGA CCATGGGATG
ACCACAAATG GAGACCATGG AGGGGACAGT GAGCTGGAGG TCTCAGCTGC TCTCTTTCTG
TATAGCCCCA CAGCAGTCTT CCCCAGCACC CCACCAGAGG AGCCAGAGGT GATTCCTCAA
GTTAGCCTTG TGCCCACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGGAATATC
GGGGAAGTGA TGGCTGAGCT ATTCTCAGGG GGTGAGGACT CCCAGCCCCA CTCCTCTGCT
TTAGCCCAAG CCTCAGCTCT CCATCTCAAT GCTCAGCAGG TGTCCCGATT TCTTCATACC
TACTCAGCTG CTACTCAGGA CCTTCAAGCT AAGGAGCTTC ATCAGCTGCA GAACCTCTTC
TCCAAGGCCT CTGCTGACTA CCAGTGGCTT CTCCAGAGCC CCAAGGGGGC TGAGGCGACA
CTGCCGACTG TGATTGCTGA GCTGCAGCAG TTCCTGCGGG GAGCTCGGGC CATGTGCATC
GAGTCTTGGG CTCGTTTCTC TCTGGTCCGC ATGGCGGGGG GTACTGCTCT CTTGGCTGCT
TCCTGCTTTA TCTGCCTGCT GGCATCTCAG TGGGCAATAT CCCCAGGCTT TCCATTCTGC
CCTCTACTCC TGACACCTGT GGCCTGGGGC CTGGTTGGGG CCATAGCGTA TGCTGGACTC
CTGGGAACTA TTGAGCTGAA GCTAGATCTA GTGCTTCTAG GGGCTGTGGC TGCAGTGAGC
TCATTCCTCC CTTTTCTGTG GAAAGCCTGG GCTGGCTGGG GGTCCAAGAG GCCCCTGGCA
ACCCTGTTTC CCATCCCTGG GCCCGTCCTG TTACTCCTGC TGTTTCGCTT GGCTGTGTTC
TTCTCTGATA GTTTTGTTGT AGCTGAGGCC AGGGCCACCC CCTTCCTTTT GGGCTCATTC
ATCCTGCTCC TGGTTGTCCA GCTTCACTGG GAGGGCCAGC TGCTTCCACC TAAGCTACTC
ACAATGCCCC GCCTTGGCAC TTCAGCCACA ACAAACCCCC CACGGCACAA TGGTGCATAT
GCCCTGAGGC TTGGAATTGG GTTGCTTTTA TGTACAAGGC TAGCTGGGCT TTTTCATCGT
TGCCCTGAAG AGACACCTGT TTGCCACTCC TCTCCCTGGC TGAGTCCTCT GGCATCCATG
GTGGGTGGTC GAGCCAAGAA TTTGTGGTAT GGAGCTTGTG TGGCGGCGCT GGTGGCCCTG
TTAGCTGCCG TGCGCTTGTG GCTTCGCCGC TATGGTAATC TCAAGAGCCC CGAGCCACCC
ATGCTCTTTG TGCGCTGGGG ACTGCCCCTA ATGGCATTGG GTACTGCTGC CTACTGGGCA
TTGGCGTCGG GGGCAGATGA GGCTCCCCCC CGTCTCCGGG TCCTGGTCTC TGGGGCATCC
ATGGTGCTGC CTCGGGCTGT AGCAGGGCTG GCTGCTTCAG GGCTCGCGCT GCTGCTCTGG
AAGCCTGTGA CAGTGCTGGT GAAGGCTGGG GCAGGCGCTC CAAGGACCAG GACTGTCCTC
ACTCCCTTCT CAGGCCCCCC CACTTCTCAA GCTGACTTGG ATTATGTGGT CCCTCAAATC
TACCGACACA TGCAGGAGGA GTTCCGGGGC CGGTTAGAGA GGACCAAATC TCAGGGTCCC
CTGACTGTGG CTGCTTATCA GTTGGGGAGT GTCTACTCAG CTGCTATGGT CACAGCCCTC
ACCCTGTTGG CCTTCCCACT TCTGCTGTTG CATGCGGAGC GCATCAGCCT TGTGTTCCTG
CTTCTGTTTC TGCAGAGCTT CCTTCTCCTA CATCTGCTTG CTGCTGGGAT ACCCGTCACC
ACCCCTGGTC CTTTTACTGT GCCATGGCAG GCAGTCTCGG CTTGGGCCCT CATGGCCACA
CAGACCTTCT ACTCCACAGG CCACCAGCCT GTCTTTCCAG CCATCCATTG GCATGCAGCC
TTCGTGGGAT TCCCAGAGGG TCATGGCTCC TGTACTTGGC TGCCTGCTTT GCTAGTGGGA
GCCAACACCT TTGCCTCCCA CCTCCTCTTT GCAGTAGGTT GCCCACTGCT CCTGCTCTGG
CCTTTCCTGT GTGAGAGTCA AGGGCTGCGG AAGAGACAGC AGCCCCCAGG GAATGAAGCT
GATGCCAGAG TCAGACCCGA GGAGGAAGAG GAGCCACTGA TGGAGATGCG GCTCCGGGAT
GCGCCTCAGC ACTTCTATGC AGCACTGCTG CAGCTGGGCC TCAAGTACCT CTTTATCCTT
GGTATTCAGA TTCTGGCCTG TGCCTTGGCA GCCTCCATCC TTCGCAGGCA TCTCATGGTC
TGGAAAGTGT TTGCCCCTAA GTTCATATTT GAGGCTGTGG GCTTCATTGT GAGCAGCGTG
GGACTTCTCC TGGGCATAGC TTTGGTGATG AGAGTGGATG GTGCTGTGAG CTCCTGGTTC
AGGCAGCTAT TTCTGGCCCA GCAGAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19171
Clone ID Related Accession (Same CDS sequence) NM_032634 , XM_005251619 , XM_011518056
Accession Version XM_011518056.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product GPI ethanolamine phosphate transferase 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008413.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGCAGAAAG CCTCAGTGTT GCTCTTCCTG GCCTGGGTCT GCTTCCTCTT CTACGCTGGC 
ATTGCCCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTTGG AGCTCACCAA CCATAGCAGC
TGCCAAGAGC CCCCAGGCCC TGGGTCCCTG CCATGGGGGA GCCAAGGGAA ACCTGGGGCC
TGCTGGATGG CTTCCCGATT TTCGCGGGTT GTGTTGGTGC TGATAGATGC TCTGCGATTT
GACTTCGCCC AGCCCCAGCA TTCACACGTG CCTAGAGAGC CTCCTGTCTC CCTACCCTTC
CTGGGCAAAC TAAGCTCCTT GCAGAGGATC CTGGAGATTC AGCCCCACCA TGCCCGGCTC
TACCGATCTC AGGTTGACCC TCCTACCACC ACCATGCAGC GCCTCAAGGC CCTCACCACT
GGCTCACTGC CTACCTTTAT TGATGCTGGT AGTAACTTCG CCAGCCACGC CATAGTGGAA
GACAATCTCA TTAAGCAGCT CACCAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT
ACCTGGAAAG ACCTTTTCCC TGGTGCTTTC TCCAAAGCTT TCTTCTTCCC ATCCTTCAAT
GTCAGAGACC TAGACACAGT GGACAATGGC ATCCTGGAAC ACCTCTACCC CACCATGGAC
AGTGGTGAAT GGGACGTGCT GATTGCTCAC TTCCTGGGTG TGGACCACTG TGGCCACAAG
CATGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATCCAG
GGACTTGTGG AGCGTCTGGA GAATGACACA CTGCTGGTAG TGGCTGGGGA CCATGGGATG
ACCACAAATG GAGACCATGG AGGGGACAGT GAGCTGGAGG TCTCAGCTGC TCTCTTTCTG
TATAGCCCCA CAGCAGTCTT CCCCAGCACC CCACCAGAGG AGCCAGAGGT GATTCCTCAA
GTTAGCCTTG TGCCCACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGGAATATC
GGGGAAGTGA TGGCTGAGCT ATTCTCAGGG GGTGAGGACT CCCAGCCCCA CTCCTCTGCT
TTAGCCCAAG CCTCAGCTCT CCATCTCAAT GCTCAGCAGG TGTCCCGATT TCTTCATACC
TACTCAGCTG CTACTCAGGA CCTTCAAGCT AAGGAGCTTC ATCAGCTGCA GAACCTCTTC
TCCAAGGCCT CTGCTGACTA CCAGTGGCTT CTCCAGAGCC CCAAGGGGGC TGAGGCGACA
CTGCCGACTG TGATTGCTGA GCTGCAGCAG TTCCTGCGGG GAGCTCGGGC CATGTGCATC
GAGTCTTGGG CTCGTTTCTC TCTGGTCCGC ATGGCGGGGG GTACTGCTCT CTTGGCTGCT
TCCTGCTTTA TCTGCCTGCT GGCATCTCAG TGGGCAATAT CCCCAGGCTT TCCATTCTGC
CCTCTACTCC TGACACCTGT GGCCTGGGGC CTGGTTGGGG CCATAGCGTA TGCTGGACTC
CTGGGAACTA TTGAGCTGAA GCTAGATCTA GTGCTTCTAG GGGCTGTGGC TGCAGTGAGC
TCATTCCTCC CTTTTCTGTG GAAAGCCTGG GCTGGCTGGG GGTCCAAGAG GCCCCTGGCA
ACCCTGTTTC CCATCCCTGG GCCCGTCCTG TTACTCCTGC TGTTTCGCTT GGCTGTGTTC
TTCTCTGATA GTTTTGTTGT AGCTGAGGCC AGGGCCACCC CCTTCCTTTT GGGCTCATTC
ATCCTGCTCC TGGTTGTCCA GCTTCACTGG GAGGGCCAGC TGCTTCCACC TAAGCTACTC
ACAATGCCCC GCCTTGGCAC TTCAGCCACA ACAAACCCCC CACGGCACAA TGGTGCATAT
GCCCTGAGGC TTGGAATTGG GTTGCTTTTA TGTACAAGGC TAGCTGGGCT TTTTCATCGT
TGCCCTGAAG AGACACCTGT TTGCCACTCC TCTCCCTGGC TGAGTCCTCT GGCATCCATG
GTGGGTGGTC GAGCCAAGAA TTTGTGGTAT GGAGCTTGTG TGGCGGCGCT GGTGGCCCTG
TTAGCTGCCG TGCGCTTGTG GCTTCGCCGC TATGGTAATC TCAAGAGCCC CGAGCCACCC
ATGCTCTTTG TGCGCTGGGG ACTGCCCCTA ATGGCATTGG GTACTGCTGC CTACTGGGCA
TTGGCGTCGG GGGCAGATGA GGCTCCCCCC CGTCTCCGGG TCCTGGTCTC TGGGGCATCC
ATGGTGCTGC CTCGGGCTGT AGCAGGGCTG GCTGCTTCAG GGCTCGCGCT GCTGCTCTGG
AAGCCTGTGA CAGTGCTGGT GAAGGCTGGG GCAGGCGCTC CAAGGACCAG GACTGTCCTC
ACTCCCTTCT CAGGCCCCCC CACTTCTCAA GCTGACTTGG ATTATGTGGT CCCTCAAATC
TACCGACACA TGCAGGAGGA GTTCCGGGGC CGGTTAGAGA GGACCAAATC TCAGGGTCCC
CTGACTGTGG CTGCTTATCA GTTGGGGAGT GTCTACTCAG CTGCTATGGT CACAGCCCTC
ACCCTGTTGG CCTTCCCACT TCTGCTGTTG CATGCGGAGC GCATCAGCCT TGTGTTCCTG
CTTCTGTTTC TGCAGAGCTT CCTTCTCCTA CATCTGCTTG CTGCTGGGAT ACCCGTCACC
ACCCCTGGTC CTTTTACTGT GCCATGGCAG GCAGTCTCGG CTTGGGCCCT CATGGCCACA
CAGACCTTCT ACTCCACAGG CCACCAGCCT GTCTTTCCAG CCATCCATTG GCATGCAGCC
TTCGTGGGAT TCCCAGAGGG TCATGGCTCC TGTACTTGGC TGCCTGCTTT GCTAGTGGGA
GCCAACACCT TTGCCTCCCA CCTCCTCTTT GCAGTAGGTT GCCCACTGCT CCTGCTCTGG
CCTTTCCTGT GTGAGAGTCA AGGGCTGCGG AAGAGACAGC AGCCCCCAGG GAATGAAGCT
GATGCCAGAG TCAGACCCGA GGAGGAAGAG GAGCCACTGA TGGAGATGCG GCTCCGGGAT
GCGCCTCAGC ACTTCTATGC AGCACTGCTG CAGCTGGGCC TCAAGTACCT CTTTATCCTT
GGTATTCAGA TTCTGGCCTG TGCCTTGGCA GCCTCCATCC TTCGCAGGCA TCTCATGGTC
TGGAAAGTGT TTGCCCCTAA GTTCATATTT GAGGCTGTGG GCTTCATTGT GAGCAGCGTG
GGACTTCTCC TGGGCATAGC TTTGGTGATG AGAGTGGATG GTGCTGTGAG CTCCTGGTTC
AGGCAGCTAT TTCTGGCCCA GCAGAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011516358.1
CDS25..3294
Misc Feature(1)148..>921(+)
Misc Feature(2)232..>882(+)
Translation

Target ORF information:

RefSeq Version XM_011518056
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011518056

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGCAGAAAG CCTCAGTGTT GCTCTTCCTG GCCTGGGTCT GCTTCCTCTT CTACGCTGGC 
ATTGCCCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTTGG AGCTCACCAA CCATAGCAGC
TGCCAAGAGC CCCCAGGCCC TGGGTCCCTG CCATGGGGGA GCCAAGGGAA ACCTGGGGCC
TGCTGGATGG CTTCCCGATT TTCGCGGGTT GTGTTGGTGC TGATAGATGC TCTGCGATTT
GACTTCGCCC AGCCCCAGCA TTCACACGTG CCTAGAGAGC CTCCTGTCTC CCTACCCTTC
CTGGGCAAAC TAAGCTCCTT GCAGAGGATC CTGGAGATTC AGCCCCACCA TGCCCGGCTC
TACCGATCTC AGGTTGACCC TCCTACCACC ACCATGCAGC GCCTCAAGGC CCTCACCACT
GGCTCACTGC CTACCTTTAT TGATGCTGGT AGTAACTTCG CCAGCCACGC CATAGTGGAA
GACAATCTCA TTAAGCAGCT CACCAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT
ACCTGGAAAG ACCTTTTCCC TGGTGCTTTC TCCAAAGCTT TCTTCTTCCC ATCCTTCAAT
GTCAGAGACC TAGACACAGT GGACAATGGC ATCCTGGAAC ACCTCTACCC CACCATGGAC
AGTGGTGAAT GGGACGTGCT GATTGCTCAC TTCCTGGGTG TGGACCACTG TGGCCACAAG
CATGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATCCAG
GGACTTGTGG AGCGTCTGGA GAATGACACA CTGCTGGTAG TGGCTGGGGA CCATGGGATG
ACCACAAATG GAGACCATGG AGGGGACAGT GAGCTGGAGG TCTCAGCTGC TCTCTTTCTG
TATAGCCCCA CAGCAGTCTT CCCCAGCACC CCACCAGAGG AGCCAGAGGT GATTCCTCAA
GTTAGCCTTG TGCCCACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGGAATATC
GGGGAAGTGA TGGCTGAGCT ATTCTCAGGG GGTGAGGACT CCCAGCCCCA CTCCTCTGCT
TTAGCCCAAG CCTCAGCTCT CCATCTCAAT GCTCAGCAGG TGTCCCGATT TCTTCATACC
TACTCAGCTG CTACTCAGGA CCTTCAAGCT AAGGAGCTTC ATCAGCTGCA GAACCTCTTC
TCCAAGGCCT CTGCTGACTA CCAGTGGCTT CTCCAGAGCC CCAAGGGGGC TGAGGCGACA
CTGCCGACTG TGATTGCTGA GCTGCAGCAG TTCCTGCGGG GAGCTCGGGC CATGTGCATC
GAGTCTTGGG CTCGTTTCTC TCTGGTCCGC ATGGCGGGGG GTACTGCTCT CTTGGCTGCT
TCCTGCTTTA TCTGCCTGCT GGCATCTCAG TGGGCAATAT CCCCAGGCTT TCCATTCTGC
CCTCTACTCC TGACACCTGT GGCCTGGGGC CTGGTTGGGG CCATAGCGTA TGCTGGACTC
CTGGGAACTA TTGAGCTGAA GCTAGATCTA GTGCTTCTAG GGGCTGTGGC TGCAGTGAGC
TCATTCCTCC CTTTTCTGTG GAAAGCCTGG GCTGGCTGGG GGTCCAAGAG GCCCCTGGCA
ACCCTGTTTC CCATCCCTGG GCCCGTCCTG TTACTCCTGC TGTTTCGCTT GGCTGTGTTC
TTCTCTGATA GTTTTGTTGT AGCTGAGGCC AGGGCCACCC CCTTCCTTTT GGGCTCATTC
ATCCTGCTCC TGGTTGTCCA GCTTCACTGG GAGGGCCAGC TGCTTCCACC TAAGCTACTC
ACAATGCCCC GCCTTGGCAC TTCAGCCACA ACAAACCCCC CACGGCACAA TGGTGCATAT
GCCCTGAGGC TTGGAATTGG GTTGCTTTTA TGTACAAGGC TAGCTGGGCT TTTTCATCGT
TGCCCTGAAG AGACACCTGT TTGCCACTCC TCTCCCTGGC TGAGTCCTCT GGCATCCATG
GTGGGTGGTC GAGCCAAGAA TTTGTGGTAT GGAGCTTGTG TGGCGGCGCT GGTGGCCCTG
TTAGCTGCCG TGCGCTTGTG GCTTCGCCGC TATGGTAATC TCAAGAGCCC CGAGCCACCC
ATGCTCTTTG TGCGCTGGGG ACTGCCCCTA ATGGCATTGG GTACTGCTGC CTACTGGGCA
TTGGCGTCGG GGGCAGATGA GGCTCCCCCC CGTCTCCGGG TCCTGGTCTC TGGGGCATCC
ATGGTGCTGC CTCGGGCTGT AGCAGGGCTG GCTGCTTCAG GGCTCGCGCT GCTGCTCTGG
AAGCCTGTGA CAGTGCTGGT GAAGGCTGGG GCAGGCGCTC CAAGGACCAG GACTGTCCTC
ACTCCCTTCT CAGGCCCCCC CACTTCTCAA GCTGACTTGG ATTATGTGGT CCCTCAAATC
TACCGACACA TGCAGGAGGA GTTCCGGGGC CGGTTAGAGA GGACCAAATC TCAGGGTCCC
CTGACTGTGG CTGCTTATCA GTTGGGGAGT GTCTACTCAG CTGCTATGGT CACAGCCCTC
ACCCTGTTGG CCTTCCCACT TCTGCTGTTG CATGCGGAGC GCATCAGCCT TGTGTTCCTG
CTTCTGTTTC TGCAGAGCTT CCTTCTCCTA CATCTGCTTG CTGCTGGGAT ACCCGTCACC
ACCCCTGGTC CTTTTACTGT GCCATGGCAG GCAGTCTCGG CTTGGGCCCT CATGGCCACA
CAGACCTTCT ACTCCACAGG CCACCAGCCT GTCTTTCCAG CCATCCATTG GCATGCAGCC
TTCGTGGGAT TCCCAGAGGG TCATGGCTCC TGTACTTGGC TGCCTGCTTT GCTAGTGGGA
GCCAACACCT TTGCCTCCCA CCTCCTCTTT GCAGTAGGTT GCCCACTGCT CCTGCTCTGG
CCTTTCCTGT GTGAGAGTCA AGGGCTGCGG AAGAGACAGC AGCCCCCAGG GAATGAAGCT
GATGCCAGAG TCAGACCCGA GGAGGAAGAG GAGCCACTGA TGGAGATGCG GCTCCGGGAT
GCGCCTCAGC ACTTCTATGC AGCACTGCTG CAGCTGGGCC TCAAGTACCT CTTTATCCTT
GGTATTCAGA TTCTGGCCTG TGCCTTGGCA GCCTCCATCC TTCGCAGGCA TCTCATGGTC
TGGAAAGTGT TTGCCCCTAA GTTCATATTT GAGGCTGTGG GCTTCATTGT GAGCAGCGTG
GGACTTCTCC TGGGCATAGC TTTGGTGATG AGAGTGGATG GTGCTGTGAG CTCCTGGTTC
AGGCAGCTAT TTCTGGCCCA GCAGAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19171
Clone ID Related Accession (Same CDS sequence) NM_032634 , XM_005251619 , XM_011518056
Accession Version NM_032634.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product GPI ethanolamine phosphate transferase 3 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR985224.1, AY358472.1, BC029271.1, BE139632.1 and BU727886.1. This sequence is a reference standard in the RefSeqGene project. On Jan 22, 2011 this sequence version replaced gi:23397647. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AL833956.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGCAGAAAG CCTCAGTGTT GCTCTTCCTG GCCTGGGTCT GCTTCCTCTT CTACGCTGGC 
ATTGCCCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTTGG AGCTCACCAA CCATAGCAGC
TGCCAAGAGC CCCCAGGCCC TGGGTCCCTG CCATGGGGGA GCCAAGGGAA ACCTGGGGCC
TGCTGGATGG CTTCCCGATT TTCGCGGGTT GTGTTGGTGC TGATAGATGC TCTGCGATTT
GACTTCGCCC AGCCCCAGCA TTCACACGTG CCTAGAGAGC CTCCTGTCTC CCTACCCTTC
CTGGGCAAAC TAAGCTCCTT GCAGAGGATC CTGGAGATTC AGCCCCACCA TGCCCGGCTC
TACCGATCTC AGGTTGACCC TCCTACCACC ACCATGCAGC GCCTCAAGGC CCTCACCACT
GGCTCACTGC CTACCTTTAT TGATGCTGGT AGTAACTTCG CCAGCCACGC CATAGTGGAA
GACAATCTCA TTAAGCAGCT CACCAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT
ACCTGGAAAG ACCTTTTCCC TGGTGCTTTC TCCAAAGCTT TCTTCTTCCC ATCCTTCAAT
GTCAGAGACC TAGACACAGT GGACAATGGC ATCCTGGAAC ACCTCTACCC CACCATGGAC
AGTGGTGAAT GGGACGTGCT GATTGCTCAC TTCCTGGGTG TGGACCACTG TGGCCACAAG
CATGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATCCAG
GGACTTGTGG AGCGTCTGGA GAATGACACA CTGCTGGTAG TGGCTGGGGA CCATGGGATG
ACCACAAATG GAGACCATGG AGGGGACAGT GAGCTGGAGG TCTCAGCTGC TCTCTTTCTG
TATAGCCCCA CAGCAGTCTT CCCCAGCACC CCACCAGAGG AGCCAGAGGT GATTCCTCAA
GTTAGCCTTG TGCCCACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGGAATATC
GGGGAAGTGA TGGCTGAGCT ATTCTCAGGG GGTGAGGACT CCCAGCCCCA CTCCTCTGCT
TTAGCCCAAG CCTCAGCTCT CCATCTCAAT GCTCAGCAGG TGTCCCGATT TCTTCATACC
TACTCAGCTG CTACTCAGGA CCTTCAAGCT AAGGAGCTTC ATCAGCTGCA GAACCTCTTC
TCCAAGGCCT CTGCTGACTA CCAGTGGCTT CTCCAGAGCC CCAAGGGGGC TGAGGCGACA
CTGCCGACTG TGATTGCTGA GCTGCAGCAG TTCCTGCGGG GAGCTCGGGC CATGTGCATC
GAGTCTTGGG CTCGTTTCTC TCTGGTCCGC ATGGCGGGGG GTACTGCTCT CTTGGCTGCT
TCCTGCTTTA TCTGCCTGCT GGCATCTCAG TGGGCAATAT CCCCAGGCTT TCCATTCTGC
CCTCTACTCC TGACACCTGT GGCCTGGGGC CTGGTTGGGG CCATAGCGTA TGCTGGACTC
CTGGGAACTA TTGAGCTGAA GCTAGATCTA GTGCTTCTAG GGGCTGTGGC TGCAGTGAGC
TCATTCCTCC CTTTTCTGTG GAAAGCCTGG GCTGGCTGGG GGTCCAAGAG GCCCCTGGCA
ACCCTGTTTC CCATCCCTGG GCCCGTCCTG TTACTCCTGC TGTTTCGCTT GGCTGTGTTC
TTCTCTGATA GTTTTGTTGT AGCTGAGGCC AGGGCCACCC CCTTCCTTTT GGGCTCATTC
ATCCTGCTCC TGGTTGTCCA GCTTCACTGG GAGGGCCAGC TGCTTCCACC TAAGCTACTC
ACAATGCCCC GCCTTGGCAC TTCAGCCACA ACAAACCCCC CACGGCACAA TGGTGCATAT
GCCCTGAGGC TTGGAATTGG GTTGCTTTTA TGTACAAGGC TAGCTGGGCT TTTTCATCGT
TGCCCTGAAG AGACACCTGT TTGCCACTCC TCTCCCTGGC TGAGTCCTCT GGCATCCATG
GTGGGTGGTC GAGCCAAGAA TTTGTGGTAT GGAGCTTGTG TGGCGGCGCT GGTGGCCCTG
TTAGCTGCCG TGCGCTTGTG GCTTCGCCGC TATGGTAATC TCAAGAGCCC CGAGCCACCC
ATGCTCTTTG TGCGCTGGGG ACTGCCCCTA ATGGCATTGG GTACTGCTGC CTACTGGGCA
TTGGCGTCGG GGGCAGATGA GGCTCCCCCC CGTCTCCGGG TCCTGGTCTC TGGGGCATCC
ATGGTGCTGC CTCGGGCTGT AGCAGGGCTG GCTGCTTCAG GGCTCGCGCT GCTGCTCTGG
AAGCCTGTGA CAGTGCTGGT GAAGGCTGGG GCAGGCGCTC CAAGGACCAG GACTGTCCTC
ACTCCCTTCT CAGGCCCCCC CACTTCTCAA GCTGACTTGG ATTATGTGGT CCCTCAAATC
TACCGACACA TGCAGGAGGA GTTCCGGGGC CGGTTAGAGA GGACCAAATC TCAGGGTCCC
CTGACTGTGG CTGCTTATCA GTTGGGGAGT GTCTACTCAG CTGCTATGGT CACAGCCCTC
ACCCTGTTGG CCTTCCCACT TCTGCTGTTG CATGCGGAGC GCATCAGCCT TGTGTTCCTG
CTTCTGTTTC TGCAGAGCTT CCTTCTCCTA CATCTGCTTG CTGCTGGGAT ACCCGTCACC
ACCCCTGGTC CTTTTACTGT GCCATGGCAG GCAGTCTCGG CTTGGGCCCT CATGGCCACA
CAGACCTTCT ACTCCACAGG CCACCAGCCT GTCTTTCCAG CCATCCATTG GCATGCAGCC
TTCGTGGGAT TCCCAGAGGG TCATGGCTCC TGTACTTGGC TGCCTGCTTT GCTAGTGGGA
GCCAACACCT TTGCCTCCCA CCTCCTCTTT GCAGTAGGTT GCCCACTGCT CCTGCTCTGG
CCTTTCCTGT GTGAGAGTCA AGGGCTGCGG AAGAGACAGC AGCCCCCAGG GAATGAAGCT
GATGCCAGAG TCAGACCCGA GGAGGAAGAG GAGCCACTGA TGGAGATGCG GCTCCGGGAT
GCGCCTCAGC ACTTCTATGC AGCACTGCTG CAGCTGGGCC TCAAGTACCT CTTTATCCTT
GGTATTCAGA TTCTGGCCTG TGCCTTGGCA GCCTCCATCC TTCGCAGGCA TCTCATGGTC
TGGAAAGTGT TTGCCCCTAA GTTCATATTT GAGGCTGTGG GCTTCATTGT GAGCAGCGTG
GGACTTCTCC TGGGCATAGC TTTGGTGATG AGAGTGGATG GTGCTGTGAG CTCCTGGTTC
AGGCAGCTAT TTCTGGCCCA GCAGAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_116023.2
CDS397..3666
Misc Feature(1)361..363(+)
Misc Feature(2)406..468(+)
Misc Feature(3)520..>1293(+)
Misc Feature(4)604..>1254(+)
Misc Feature(5)1765..1827(+)
Misc Feature(6)1840..1902(+)
Misc Feature(7)1924..1986(+)
Misc Feature(8)2017..2079(+)
Misc Feature(9)2119..2181(+)
Misc Feature(10)2398..2460(+)
Misc Feature(11)2497..2559(+)
Misc Feature(12)2635..2697(+)
Misc Feature(13)2884..2946(+)
Misc Feature(14)2965..3027(+)
Misc Feature(15)3226..3288(+)
Misc Feature(16)3436..3498(+)
Misc Feature(17)3538..3600(+)
Exon (1)1..395
Gene:PIGO
Gene Synonym:
Exon (2)396..907
Gene:PIGO
Gene Synonym:
Exon (3)908..1051
Gene:PIGO
Gene Synonym:
Exon (4)1052..1175
Gene:PIGO
Gene Synonym:
Exon (5)1176..1335
Gene:PIGO
Gene Synonym:
Exon (6)1336..1515
Gene:PIGO
Gene Synonym:
Exon (7)1516..3043
Gene:PIGO
Gene Synonym:
Exon (8)3044..3250
Gene:PIGO
Gene Synonym:
Exon (9)3251..3465
Gene:PIGO
Gene Synonym:
Exon (10)3466..3536
Gene:PIGO
Gene Synonym:
Exon (11)3537..4070
Gene:PIGO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_032634
Organism Homo sapiens (human)
Definition Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_032634

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGCAGAAAG CCTCAGTGTT GCTCTTCCTG GCCTGGGTCT GCTTCCTCTT CTACGCTGGC 
ATTGCCCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTTGG AGCTCACCAA CCATAGCAGC
TGCCAAGAGC CCCCAGGCCC TGGGTCCCTG CCATGGGGGA GCCAAGGGAA ACCTGGGGCC
TGCTGGATGG CTTCCCGATT TTCGCGGGTT GTGTTGGTGC TGATAGATGC TCTGCGATTT
GACTTCGCCC AGCCCCAGCA TTCACACGTG CCTAGAGAGC CTCCTGTCTC CCTACCCTTC
CTGGGCAAAC TAAGCTCCTT GCAGAGGATC CTGGAGATTC AGCCCCACCA TGCCCGGCTC
TACCGATCTC AGGTTGACCC TCCTACCACC ACCATGCAGC GCCTCAAGGC CCTCACCACT
GGCTCACTGC CTACCTTTAT TGATGCTGGT AGTAACTTCG CCAGCCACGC CATAGTGGAA
GACAATCTCA TTAAGCAGCT CACCAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT
ACCTGGAAAG ACCTTTTCCC TGGTGCTTTC TCCAAAGCTT TCTTCTTCCC ATCCTTCAAT
GTCAGAGACC TAGACACAGT GGACAATGGC ATCCTGGAAC ACCTCTACCC CACCATGGAC
AGTGGTGAAT GGGACGTGCT GATTGCTCAC TTCCTGGGTG TGGACCACTG TGGCCACAAG
CATGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATCCAG
GGACTTGTGG AGCGTCTGGA GAATGACACA CTGCTGGTAG TGGCTGGGGA CCATGGGATG
ACCACAAATG GAGACCATGG AGGGGACAGT GAGCTGGAGG TCTCAGCTGC TCTCTTTCTG
TATAGCCCCA CAGCAGTCTT CCCCAGCACC CCACCAGAGG AGCCAGAGGT GATTCCTCAA
GTTAGCCTTG TGCCCACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGGAATATC
GGGGAAGTGA TGGCTGAGCT ATTCTCAGGG GGTGAGGACT CCCAGCCCCA CTCCTCTGCT
TTAGCCCAAG CCTCAGCTCT CCATCTCAAT GCTCAGCAGG TGTCCCGATT TCTTCATACC
TACTCAGCTG CTACTCAGGA CCTTCAAGCT AAGGAGCTTC ATCAGCTGCA GAACCTCTTC
TCCAAGGCCT CTGCTGACTA CCAGTGGCTT CTCCAGAGCC CCAAGGGGGC TGAGGCGACA
CTGCCGACTG TGATTGCTGA GCTGCAGCAG TTCCTGCGGG GAGCTCGGGC CATGTGCATC
GAGTCTTGGG CTCGTTTCTC TCTGGTCCGC ATGGCGGGGG GTACTGCTCT CTTGGCTGCT
TCCTGCTTTA TCTGCCTGCT GGCATCTCAG TGGGCAATAT CCCCAGGCTT TCCATTCTGC
CCTCTACTCC TGACACCTGT GGCCTGGGGC CTGGTTGGGG CCATAGCGTA TGCTGGACTC
CTGGGAACTA TTGAGCTGAA GCTAGATCTA GTGCTTCTAG GGGCTGTGGC TGCAGTGAGC
TCATTCCTCC CTTTTCTGTG GAAAGCCTGG GCTGGCTGGG GGTCCAAGAG GCCCCTGGCA
ACCCTGTTTC CCATCCCTGG GCCCGTCCTG TTACTCCTGC TGTTTCGCTT GGCTGTGTTC
TTCTCTGATA GTTTTGTTGT AGCTGAGGCC AGGGCCACCC CCTTCCTTTT GGGCTCATTC
ATCCTGCTCC TGGTTGTCCA GCTTCACTGG GAGGGCCAGC TGCTTCCACC TAAGCTACTC
ACAATGCCCC GCCTTGGCAC TTCAGCCACA ACAAACCCCC CACGGCACAA TGGTGCATAT
GCCCTGAGGC TTGGAATTGG GTTGCTTTTA TGTACAAGGC TAGCTGGGCT TTTTCATCGT
TGCCCTGAAG AGACACCTGT TTGCCACTCC TCTCCCTGGC TGAGTCCTCT GGCATCCATG
GTGGGTGGTC GAGCCAAGAA TTTGTGGTAT GGAGCTTGTG TGGCGGCGCT GGTGGCCCTG
TTAGCTGCCG TGCGCTTGTG GCTTCGCCGC TATGGTAATC TCAAGAGCCC CGAGCCACCC
ATGCTCTTTG TGCGCTGGGG ACTGCCCCTA ATGGCATTGG GTACTGCTGC CTACTGGGCA
TTGGCGTCGG GGGCAGATGA GGCTCCCCCC CGTCTCCGGG TCCTGGTCTC TGGGGCATCC
ATGGTGCTGC CTCGGGCTGT AGCAGGGCTG GCTGCTTCAG GGCTCGCGCT GCTGCTCTGG
AAGCCTGTGA CAGTGCTGGT GAAGGCTGGG GCAGGCGCTC CAAGGACCAG GACTGTCCTC
ACTCCCTTCT CAGGCCCCCC CACTTCTCAA GCTGACTTGG ATTATGTGGT CCCTCAAATC
TACCGACACA TGCAGGAGGA GTTCCGGGGC CGGTTAGAGA GGACCAAATC TCAGGGTCCC
CTGACTGTGG CTGCTTATCA GTTGGGGAGT GTCTACTCAG CTGCTATGGT CACAGCCCTC
ACCCTGTTGG CCTTCCCACT TCTGCTGTTG CATGCGGAGC GCATCAGCCT TGTGTTCCTG
CTTCTGTTTC TGCAGAGCTT CCTTCTCCTA CATCTGCTTG CTGCTGGGAT ACCCGTCACC
ACCCCTGGTC CTTTTACTGT GCCATGGCAG GCAGTCTCGG CTTGGGCCCT CATGGCCACA
CAGACCTTCT ACTCCACAGG CCACCAGCCT GTCTTTCCAG CCATCCATTG GCATGCAGCC
TTCGTGGGAT TCCCAGAGGG TCATGGCTCC TGTACTTGGC TGCCTGCTTT GCTAGTGGGA
GCCAACACCT TTGCCTCCCA CCTCCTCTTT GCAGTAGGTT GCCCACTGCT CCTGCTCTGG
CCTTTCCTGT GTGAGAGTCA AGGGCTGCGG AAGAGACAGC AGCCCCCAGG GAATGAAGCT
GATGCCAGAG TCAGACCCGA GGAGGAAGAG GAGCCACTGA TGGAGATGCG GCTCCGGGAT
GCGCCTCAGC ACTTCTATGC AGCACTGCTG CAGCTGGGCC TCAAGTACCT CTTTATCCTT
GGTATTCAGA TTCTGGCCTG TGCCTTGGCA GCCTCCATCC TTCGCAGGCA TCTCATGGTC
TGGAAAGTGT TTGCCCCTAA GTTCATATTT GAGGCTGTGG GCTTCATTGT GAGCAGCGTG
GGACTTCTCC TGGGCATAGC TTTGGTGATG AGAGTGGATG GTGCTGTGAG CTCCTGGTTC
AGGCAGCTAT TTCTGGCCCA GCAGAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15672
Clone ID Related Accession (Same CDS sequence) NM_001201484 , NM_152850
Accession Version NM_152850.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product GPI ethanolamine phosphate transferase 3 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR991745.1, BC036916.1, BE139632.1 and BU727886.1. On Jan 22, 2011 this sequence version replaced gi:38045916. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (2) has the same N- and C-termini but lacks an internal segment compared to isoform 1. Variants 2 and 3 both encode the same isoform (2). ##Evidence-Data-START## CDS exon combination :: BC036916.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCAGAAAG CCTCAGTGTT GCTCTTCCTG GCCTGGGTCT GCTTCCTCTT CTACGCTGGC 
ATTGCCCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTTGG AGCTCACCAA CCATAGCAGC
TGCCAAGAGC CCCCAGGCCC TGGGTCCCTG CCATGGGGGA GCCAAGGGAA ACCTGGGGCC
TGCTGGATGG CTTCCCGATT TTCGCGGGTT GTGTTGGTGC TGATAGATGC TCTGCGATTT
GACTTCGCCC AGCCCCAGCA TTCACACGTG CCTAGAGAGC CTCCTGTCTC CCTACCCTTC
CTGGGCAAAC TAAGCTCCTT GCAGAGGATC CTGGAGATTC AGCCCCACCA TGCCCGGCTC
TACCGATCTC AGGTTGACCC TCCTACCACC ACCATGCAGC GCCTCAAGGC CCTCACCACT
GGCTCACTGC CTACCTTTAT TGATGCTGGT AGTAACTTCG CCAGCCACGC CATAGTGGAA
GACAATCTCA TTAAGCAGCT CACCAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT
ACCTGGAAAG ACCTTTTCCC TGGTGCTTTC TCCAAAGCTT TCTTCTTCCC ATCCTTCAAT
GTCAGAGACC TAGACACAGT GGACAATGGC ATCCTGGAAC ACCTCTACCC CACCATGGAC
AGTGGTGAAT GGGACGTGCT GATTGCTCAC TTCCTGGGTG TGGACCACTG TGGCCACAAG
CATGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATCCAG
GGACTTGTGG AGCGTCTGGA GAATGACACA CTGCTGGTAG TGGCTGGGGA CCATGGGATG
ACCACAAATG GAGACCATGG AGGGGACAGT GAGCTGGAGG TCTCAGCTGC TCTCTTTCTG
TATAGCCCCA CAGCAGTCTT CCCCAGCACC CCACCAGAGG AGCCAGAGGT GATTCCTCAA
GTTAGCCTTG TGCCCACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGGAATATC
GGGGAAGTGA TGGCTGAGCT ATTCTCAGGG GGTGAGGACT CCCAGCCCCA CTCCTCTGCT
TTAGCCCAAG CCTCAGCTCT CCATCTCAAT GCTCAGCAGG TGTCCCGATT TCTTCATACC
TACTCAGCTG CTACTCAGGA CCTTCAAGCT AAGGAGCTTC ATCAGCTGCA GAACCTCTTC
TCCAAGGCCT CTGCTGACTA CCAGTGGCTT CTCCAGAGCC CCAAGGGGGC TGAGGCGACA
CTGCCGACTG TGATTGCTGA GCTGCAGCAG TTCCTGCGGG GAGCTCGGGC CATGTGCATC
GAGTCTTGGG CTCGTTTCTC TCTGAGCTTC CTTCTCCTAC ATCTGCTTGC TGCTGGGATA
CCCGTCACCA CCCCTGGTCC TTTTACTGTG CCATGGCAGG CAGTCTCGGC TTGGGCCCTC
ATGGCCACAC AGACCTTCTA CTCCACAGGC CACCAGCCTG TCTTTCCAGC CATCCATTGG
CATGCAGCCT TCGTGGGATT CCCAGAGGGT CATGGCTCCT GTACTTGGCT GCCTGCTTTG
CTAGTGGGAG CCAACACCTT TGCCTCCCAC CTCCTCTTTG CAGTAGGTTG CCCACTGCTC
CTGCTCTGGC CTTTCCTGTG TGAGAGTCAA GGGCTGCGGA AGAGACAGCA GCCCCCAGGG
AATGAAGCTG ATGCCAGAGT CAGACCCGAG GAGGAAGAGG AGCCACTGAT GGAGATGCGG
CTCCGGGATG CGCCTCAGCA CTTCTATGCA GCACTGCTGC AGCTGGGCCT CAAGTACCTC
TTTATCCTTG GTATTCAGAT TCTGGCCTGT GCCTTGGCAG CCTCCATCCT TCGCAGGCAT
CTCATGGTCT GGAAAGTGTT TGCCCCTAAG TTCATATTTG AGGCTGTGGG CTTCATTGTG
AGCAGCGTGG GACTTCTCCT GGGCATAGCT TTGGTGATGA GAGTGGATGG TGCTGTGAGC
TCCTGGTTCA GGCAGCTATT TCTGGCCCAG CAGAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_690577.2
CDS43..2061
Misc Feature(1)52..114(+)
Misc Feature(2)166..>939(+)
Misc Feature(3)250..>900(+)
Exon (1)1..41
Gene:PIGO
Gene Synonym:
Exon (2)42..553
Gene:PIGO
Gene Synonym:
Exon (3)554..697
Gene:PIGO
Gene Synonym:
Exon (4)698..821
Gene:PIGO
Gene Synonym:
Exon (5)822..981
Gene:PIGO
Gene Synonym:
Exon (6)982..1161
Gene:PIGO
Gene Synonym:
Exon (7)1162..1386
Gene:PIGO
Gene Synonym:
Exon (8)1387..1438
Gene:PIGO
Gene Synonym:
Exon (9)1439..1645
Gene:PIGO
Gene Synonym:
Exon (10)1646..1860
Gene:PIGO
Gene Synonym:
Exon (11)1861..1931
Gene:PIGO
Gene Synonym:
Exon (12)1932..2465
Gene:PIGO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_152850
Organism Homo sapiens (human)
Definition Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_152850

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCAGAAAG CCTCAGTGTT GCTCTTCCTG GCCTGGGTCT GCTTCCTCTT CTACGCTGGC 
ATTGCCCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTTGG AGCTCACCAA CCATAGCAGC
TGCCAAGAGC CCCCAGGCCC TGGGTCCCTG CCATGGGGGA GCCAAGGGAA ACCTGGGGCC
TGCTGGATGG CTTCCCGATT TTCGCGGGTT GTGTTGGTGC TGATAGATGC TCTGCGATTT
GACTTCGCCC AGCCCCAGCA TTCACACGTG CCTAGAGAGC CTCCTGTCTC CCTACCCTTC
CTGGGCAAAC TAAGCTCCTT GCAGAGGATC CTGGAGATTC AGCCCCACCA TGCCCGGCTC
TACCGATCTC AGGTTGACCC TCCTACCACC ACCATGCAGC GCCTCAAGGC CCTCACCACT
GGCTCACTGC CTACCTTTAT TGATGCTGGT AGTAACTTCG CCAGCCACGC CATAGTGGAA
GACAATCTCA TTAAGCAGCT CACCAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT
ACCTGGAAAG ACCTTTTCCC TGGTGCTTTC TCCAAAGCTT TCTTCTTCCC ATCCTTCAAT
GTCAGAGACC TAGACACAGT GGACAATGGC ATCCTGGAAC ACCTCTACCC CACCATGGAC
AGTGGTGAAT GGGACGTGCT GATTGCTCAC TTCCTGGGTG TGGACCACTG TGGCCACAAG
CATGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATCCAG
GGACTTGTGG AGCGTCTGGA GAATGACACA CTGCTGGTAG TGGCTGGGGA CCATGGGATG
ACCACAAATG GAGACCATGG AGGGGACAGT GAGCTGGAGG TCTCAGCTGC TCTCTTTCTG
TATAGCCCCA CAGCAGTCTT CCCCAGCACC CCACCAGAGG AGCCAGAGGT GATTCCTCAA
GTTAGCCTTG TGCCCACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGGAATATC
GGGGAAGTGA TGGCTGAGCT ATTCTCAGGG GGTGAGGACT CCCAGCCCCA CTCCTCTGCT
TTAGCCCAAG CCTCAGCTCT CCATCTCAAT GCTCAGCAGG TGTCCCGATT TCTTCATACC
TACTCAGCTG CTACTCAGGA CCTTCAAGCT AAGGAGCTTC ATCAGCTGCA GAACCTCTTC
TCCAAGGCCT CTGCTGACTA CCAGTGGCTT CTCCAGAGCC CCAAGGGGGC TGAGGCGACA
CTGCCGACTG TGATTGCTGA GCTGCAGCAG TTCCTGCGGG GAGCTCGGGC CATGTGCATC
GAGTCTTGGG CTCGTTTCTC TCTGAGCTTC CTTCTCCTAC ATCTGCTTGC TGCTGGGATA
CCCGTCACCA CCCCTGGTCC TTTTACTGTG CCATGGCAGG CAGTCTCGGC TTGGGCCCTC
ATGGCCACAC AGACCTTCTA CTCCACAGGC CACCAGCCTG TCTTTCCAGC CATCCATTGG
CATGCAGCCT TCGTGGGATT CCCAGAGGGT CATGGCTCCT GTACTTGGCT GCCTGCTTTG
CTAGTGGGAG CCAACACCTT TGCCTCCCAC CTCCTCTTTG CAGTAGGTTG CCCACTGCTC
CTGCTCTGGC CTTTCCTGTG TGAGAGTCAA GGGCTGCGGA AGAGACAGCA GCCCCCAGGG
AATGAAGCTG ATGCCAGAGT CAGACCCGAG GAGGAAGAGG AGCCACTGAT GGAGATGCGG
CTCCGGGATG CGCCTCAGCA CTTCTATGCA GCACTGCTGC AGCTGGGCCT CAAGTACCTC
TTTATCCTTG GTATTCAGAT TCTGGCCTGT GCCTTGGCAG CCTCCATCCT TCGCAGGCAT
CTCATGGTCT GGAAAGTGTT TGCCCCTAAG TTCATATTTG AGGCTGTGGG CTTCATTGTG
AGCAGCGTGG GACTTCTCCT GGGCATAGCT TTGGTGATGA GAGTGGATGG TGCTGTGAGC
TCCTGGTTCA GGCAGCTATT TCTGGCCCAG CAGAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15672
Clone ID Related Accession (Same CDS sequence) NM_001201484 , NM_152850
Accession Version NM_001201484.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product GPI ethanolamine phosphate transferase 3 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR985224.1, BC065282.1, BE139632.1 and BU727886.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (2) has the same N- and C-termini but lacks an internal segment compared to isoform 1. Variants 2 and 3 both encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: BC065282.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCAGAAAG CCTCAGTGTT GCTCTTCCTG GCCTGGGTCT GCTTCCTCTT CTACGCTGGC 
ATTGCCCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTTGG AGCTCACCAA CCATAGCAGC
TGCCAAGAGC CCCCAGGCCC TGGGTCCCTG CCATGGGGGA GCCAAGGGAA ACCTGGGGCC
TGCTGGATGG CTTCCCGATT TTCGCGGGTT GTGTTGGTGC TGATAGATGC TCTGCGATTT
GACTTCGCCC AGCCCCAGCA TTCACACGTG CCTAGAGAGC CTCCTGTCTC CCTACCCTTC
CTGGGCAAAC TAAGCTCCTT GCAGAGGATC CTGGAGATTC AGCCCCACCA TGCCCGGCTC
TACCGATCTC AGGTTGACCC TCCTACCACC ACCATGCAGC GCCTCAAGGC CCTCACCACT
GGCTCACTGC CTACCTTTAT TGATGCTGGT AGTAACTTCG CCAGCCACGC CATAGTGGAA
GACAATCTCA TTAAGCAGCT CACCAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT
ACCTGGAAAG ACCTTTTCCC TGGTGCTTTC TCCAAAGCTT TCTTCTTCCC ATCCTTCAAT
GTCAGAGACC TAGACACAGT GGACAATGGC ATCCTGGAAC ACCTCTACCC CACCATGGAC
AGTGGTGAAT GGGACGTGCT GATTGCTCAC TTCCTGGGTG TGGACCACTG TGGCCACAAG
CATGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATCCAG
GGACTTGTGG AGCGTCTGGA GAATGACACA CTGCTGGTAG TGGCTGGGGA CCATGGGATG
ACCACAAATG GAGACCATGG AGGGGACAGT GAGCTGGAGG TCTCAGCTGC TCTCTTTCTG
TATAGCCCCA CAGCAGTCTT CCCCAGCACC CCACCAGAGG AGCCAGAGGT GATTCCTCAA
GTTAGCCTTG TGCCCACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGGAATATC
GGGGAAGTGA TGGCTGAGCT ATTCTCAGGG GGTGAGGACT CCCAGCCCCA CTCCTCTGCT
TTAGCCCAAG CCTCAGCTCT CCATCTCAAT GCTCAGCAGG TGTCCCGATT TCTTCATACC
TACTCAGCTG CTACTCAGGA CCTTCAAGCT AAGGAGCTTC ATCAGCTGCA GAACCTCTTC
TCCAAGGCCT CTGCTGACTA CCAGTGGCTT CTCCAGAGCC CCAAGGGGGC TGAGGCGACA
CTGCCGACTG TGATTGCTGA GCTGCAGCAG TTCCTGCGGG GAGCTCGGGC CATGTGCATC
GAGTCTTGGG CTCGTTTCTC TCTGAGCTTC CTTCTCCTAC ATCTGCTTGC TGCTGGGATA
CCCGTCACCA CCCCTGGTCC TTTTACTGTG CCATGGCAGG CAGTCTCGGC TTGGGCCCTC
ATGGCCACAC AGACCTTCTA CTCCACAGGC CACCAGCCTG TCTTTCCAGC CATCCATTGG
CATGCAGCCT TCGTGGGATT CCCAGAGGGT CATGGCTCCT GTACTTGGCT GCCTGCTTTG
CTAGTGGGAG CCAACACCTT TGCCTCCCAC CTCCTCTTTG CAGTAGGTTG CCCACTGCTC
CTGCTCTGGC CTTTCCTGTG TGAGAGTCAA GGGCTGCGGA AGAGACAGCA GCCCCCAGGG
AATGAAGCTG ATGCCAGAGT CAGACCCGAG GAGGAAGAGG AGCCACTGAT GGAGATGCGG
CTCCGGGATG CGCCTCAGCA CTTCTATGCA GCACTGCTGC AGCTGGGCCT CAAGTACCTC
TTTATCCTTG GTATTCAGAT TCTGGCCTGT GCCTTGGCAG CCTCCATCCT TCGCAGGCAT
CTCATGGTCT GGAAAGTGTT TGCCCCTAAG TTCATATTTG AGGCTGTGGG CTTCATTGTG
AGCAGCGTGG GACTTCTCCT GGGCATAGCT TTGGTGATGA GAGTGGATGG TGCTGTGAGC
TCCTGGTTCA GGCAGCTATT TCTGGCCCAG CAGAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001188413.1
CDS495..2513
Misc Feature(1)483..485(+)
Misc Feature(2)504..566(+)
Misc Feature(3)618..>1391(+)
Misc Feature(4)702..>1352(+)
Exon (1)1..395
Gene:PIGO
Gene Synonym:
Exon (2)396..493
Gene:PIGO
Gene Synonym:
Exon (3)494..1005
Gene:PIGO
Gene Synonym:
Exon (4)1006..1149
Gene:PIGO
Gene Synonym:
Exon (5)1150..1273
Gene:PIGO
Gene Synonym:
Exon (6)1274..1433
Gene:PIGO
Gene Synonym:
Exon (7)1434..1613
Gene:PIGO
Gene Synonym:
Exon (8)1614..1838
Gene:PIGO
Gene Synonym:
Exon (9)1839..1890
Gene:PIGO
Gene Synonym:
Exon (10)1891..2097
Gene:PIGO
Gene Synonym:
Exon (11)2098..2312
Gene:PIGO
Gene Synonym:
Exon (12)2313..2383
Gene:PIGO
Gene Synonym:
Exon (13)2384..2917
Gene:PIGO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001201484
Organism Homo sapiens (human)
Definition Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001201484

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCAGAAAG CCTCAGTGTT GCTCTTCCTG GCCTGGGTCT GCTTCCTCTT CTACGCTGGC 
ATTGCCCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTTGG AGCTCACCAA CCATAGCAGC
TGCCAAGAGC CCCCAGGCCC TGGGTCCCTG CCATGGGGGA GCCAAGGGAA ACCTGGGGCC
TGCTGGATGG CTTCCCGATT TTCGCGGGTT GTGTTGGTGC TGATAGATGC TCTGCGATTT
GACTTCGCCC AGCCCCAGCA TTCACACGTG CCTAGAGAGC CTCCTGTCTC CCTACCCTTC
CTGGGCAAAC TAAGCTCCTT GCAGAGGATC CTGGAGATTC AGCCCCACCA TGCCCGGCTC
TACCGATCTC AGGTTGACCC TCCTACCACC ACCATGCAGC GCCTCAAGGC CCTCACCACT
GGCTCACTGC CTACCTTTAT TGATGCTGGT AGTAACTTCG CCAGCCACGC CATAGTGGAA
GACAATCTCA TTAAGCAGCT CACCAGTGCA GGAAGGCGTG TAGTCTTCAT GGGAGATGAT
ACCTGGAAAG ACCTTTTCCC TGGTGCTTTC TCCAAAGCTT TCTTCTTCCC ATCCTTCAAT
GTCAGAGACC TAGACACAGT GGACAATGGC ATCCTGGAAC ACCTCTACCC CACCATGGAC
AGTGGTGAAT GGGACGTGCT GATTGCTCAC TTCCTGGGTG TGGACCACTG TGGCCACAAG
CATGGCCCTC ACCACCCTGA AATGGCCAAG AAACTTAGCC AGATGGACCA GGTGATCCAG
GGACTTGTGG AGCGTCTGGA GAATGACACA CTGCTGGTAG TGGCTGGGGA CCATGGGATG
ACCACAAATG GAGACCATGG AGGGGACAGT GAGCTGGAGG TCTCAGCTGC TCTCTTTCTG
TATAGCCCCA CAGCAGTCTT CCCCAGCACC CCACCAGAGG AGCCAGAGGT GATTCCTCAA
GTTAGCCTTG TGCCCACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGGAATATC
GGGGAAGTGA TGGCTGAGCT ATTCTCAGGG GGTGAGGACT CCCAGCCCCA CTCCTCTGCT
TTAGCCCAAG CCTCAGCTCT CCATCTCAAT GCTCAGCAGG TGTCCCGATT TCTTCATACC
TACTCAGCTG CTACTCAGGA CCTTCAAGCT AAGGAGCTTC ATCAGCTGCA GAACCTCTTC
TCCAAGGCCT CTGCTGACTA CCAGTGGCTT CTCCAGAGCC CCAAGGGGGC TGAGGCGACA
CTGCCGACTG TGATTGCTGA GCTGCAGCAG TTCCTGCGGG GAGCTCGGGC CATGTGCATC
GAGTCTTGGG CTCGTTTCTC TCTGAGCTTC CTTCTCCTAC ATCTGCTTGC TGCTGGGATA
CCCGTCACCA CCCCTGGTCC TTTTACTGTG CCATGGCAGG CAGTCTCGGC TTGGGCCCTC
ATGGCCACAC AGACCTTCTA CTCCACAGGC CACCAGCCTG TCTTTCCAGC CATCCATTGG
CATGCAGCCT TCGTGGGATT CCCAGAGGGT CATGGCTCCT GTACTTGGCT GCCTGCTTTG
CTAGTGGGAG CCAACACCTT TGCCTCCCAC CTCCTCTTTG CAGTAGGTTG CCCACTGCTC
CTGCTCTGGC CTTTCCTGTG TGAGAGTCAA GGGCTGCGGA AGAGACAGCA GCCCCCAGGG
AATGAAGCTG ATGCCAGAGT CAGACCCGAG GAGGAAGAGG AGCCACTGAT GGAGATGCGG
CTCCGGGATG CGCCTCAGCA CTTCTATGCA GCACTGCTGC AGCTGGGCCT CAAGTACCTC
TTTATCCTTG GTATTCAGAT TCTGGCCTGT GCCTTGGCAG CCTCCATCCT TCGCAGGCAT
CTCATGGTCT GGAAAGTGTT TGCCCCTAAG TTCATATTTG AGGCTGTGGG CTTCATTGTG
AGCAGCGTGG GACTTCTCCT GGGCATAGCT TTGGTGATGA GAGTGGATGG TGCTGTGAGC
TCCTGGTTCA GGCAGCTATT TCTGGCCCAG CAGAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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