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B3GALT1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol B3GALT1
Entrez Gene ID 8708
Full Name UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1
Synonyms beta3Gal-T1
General protein information
Preferred Names
beta-1,3-galactosyltransferase 1
Names
beta-1,3-galactosyltransferase 1
beta3GalT1
beta-3-galt1
beta-1,3-GalTase 1
UDP-galactose:beta-N-acetyl-glucosamine-beta-1,3-galactosyltransferase 1
NP_066191.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q24.3

Summary This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is expressed exclusively in the brain. The encoded protein shows strict donor substrate specificity for UDP-galactose. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

603093


mRNA and Protein(s)

mRNA Protein Name
XM_005246931 XP_005246988 beta-1,3-galactosyltransferase 1 isoform X1
XM_011512084 XP_011510386 beta-1,3-galactosyltransferase 1 isoform X1
XM_006712819 XP_006712882 beta-1,3-galactosyltransferase 1 isoform X1
XM_011512085 XP_011510387 beta-1,3-galactosyltransferase 1 isoform X1
NM_020981 NP_066191 beta-1,3-galactosyltransferase 1



Homo sapiens (human) B3GALT1 NP_066191.1
Pan troglodytes (chimpanzee) B3GALT1 NP_001009096.1
Macaca mulatta (Rhesus monkey) B3GALT1 XP_001102033.1
Canis lupus familiaris (dog) B3GALT1 XP_005640327.1
Bos taurus (cattle) B3GALT1 XP_005202457.1
Mus musculus (house mouse) B3galt1 NP_064679.2
Rattus norvegicus (Norway rat) B3galt1 NP_001102424.1
Gallus gallus (chicken) B3GALT1 XP_004942835.1
Danio rerio (zebrafish) LOC571003 XP_005172591.1
Drosophila melanogaster (fruit fly) CG8668 NP_609184.1
Arabidopsis thaliana (thale cress) AT3G06440 NP_566284.1
Xenopus (Silurana) tropicalis (western clawed frog) b3galt1 XP_004917762.1


The following B3GALT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B3GALT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu11144
XM_005246931 PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu11144
XM_011512084 PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu11144
XM_006712819 PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu11144
XM_011512085 PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu11144
NM_020981 Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu11144
Clone ID Related Accession (Same CDS sequence) NM_020981 , XM_005246931 , XM_006712819 , XM_011512084 , XM_011512085
Accession Version XM_005246931.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-1,3-galactosyltransferase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530371118. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005246988.1
CDS785..1765
Misc Feature(1)1058..1621(+)
Translation

Target ORF information:

RefSeq Version XM_005246931
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005246931

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu11144
Clone ID Related Accession (Same CDS sequence) NM_020981 , XM_005246931 , XM_006712819 , XM_011512084 , XM_011512085
Accession Version XM_011512084.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-1,3-galactosyltransferase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510386.1
CDS530..1510
Misc Feature(1)803..1366(+)
Translation

Target ORF information:

RefSeq Version XM_011512084
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011512084

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu11144
Clone ID Related Accession (Same CDS sequence) NM_020981 , XM_005246931 , XM_006712819 , XM_011512084 , XM_011512085
Accession Version XM_006712819.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-1,3-galactosyltransferase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578805076. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006712882.1
CDS548..1528
Misc Feature(1)821..1384(+)
Translation

Target ORF information:

RefSeq Version XM_006712819
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006712819

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu11144
Clone ID Related Accession (Same CDS sequence) NM_020981 , XM_005246931 , XM_006712819 , XM_011512084 , XM_011512085
Accession Version XM_011512085.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-1,3-galactosyltransferase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510387.1
CDS801..1781
Misc Feature(1)1074..1637(+)
Translation

Target ORF information:

RefSeq Version XM_011512085
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011512085

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu11144
Clone ID Related Accession (Same CDS sequence) NM_020981 , XM_005246931 , XM_006712819 , XM_011512084 , XM_011512085
Accession Version NM_020981.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product beta-1,3-galactosyltransferase 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC016723.11 and AF117222.1. On Sep 3, 2010 this sequence version replaced gi:15451870. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: AF117222.1, BC101545.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_066191.1
CDS352..1332
Misc Feature(1)298..300(+)
Misc Feature(2)370..429(+)
Misc Feature(3)625..1188(+)
Exon (1)1..122
Gene:B3GALT1
Gene Synonym:
Exon (2)123..2168
Gene:B3GALT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_020981
Organism Homo sapiens (human)
Definition Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020981

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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