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B3GALT1 UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 [Homo sapiens (human)]

Gene Symbol B3GALT1
Entrez Gene ID 8708
Full Name UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1
Synonyms beta3Gal-T1
General protein information
Preferred Names
beta-1,3-galactosyltransferase 1
Names
beta-1,3-galactosyltransferase 1
beta3GalT1
beta-3-galt1
beta-1,3-GalTase 1
UDP-galactose:beta-N-acetyl-glucosamine-beta-1,3-galactosyltransferase 1
NP_066191.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q24.3

Summary This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is expressed exclusively in the brain. The encoded protein shows strict donor substrate specificity for UDP-galactose. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

603093

Disorder Html:
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu11144 XM_005246931 PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu11144 XM_011512084 PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu11144 XM_006712819 PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu11144 XM_011512085 PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu11144 NM_020981 Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu11144D
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-1,3-galactosyltransferase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530371118. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTTA G
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005246988.1
CDS785..1765
Misc Feature(1)1058..1621(+)
Translation
Position Chain Variation Link
1 1 c, g dbSNP:140101666
3 3 a, g dbSNP:527637881
30 30 g, t dbSNP:111275619
45 45 c, t dbSNP:546965326
84 84 g, t dbSNP:566784392
89 89 g, t dbSNP:535927288
102 102 a, g dbSNP:549468288
118 118 a, g dbSNP:569387320
124 124 a, g dbSNP:538399879
196 196 c, t dbSNP:558071059
205 205 c, g dbSNP:143685188
216 216 c, t dbSNP:578193770
260 260 c, g dbSNP:529493033
272 272 c, t dbSNP:373675441
279 279 a, g dbSNP:189283865
287 287 c, t dbSNP:180869998
288 288 a, g dbSNP:778038057
290 290 c, t dbSNP:142417380
309 309 a, g dbSNP:749625200
331 331 a, g dbSNP:568899703
361 361 a, g dbSNP:533716892
413 413 a, g dbSNP:79068432
417 417 a, g dbSNP:75814609
420 420 a, g dbSNP:114352176
440 440 a, g dbSNP:836722
457 457 c, t dbSNP:527551808
458 458 a, g dbSNP:836723
463 463 -, c dbSNP:750306272
477 477 -, ctc dbSNP:72249429
513 513 c, t dbSNP:773332197
516 516 c, t dbSNP:749338474
518 518 a, g dbSNP:13396351
541 541 a, c, t dbSNP:529424683
542 542 a, g dbSNP:549484614
555 555 a, g dbSNP:569501938
560 560 a, t dbSNP:552120919
570 570 c, t dbSNP:562616878
601 601 g, t dbSNP:777239799
610 610 g, t dbSNP:768161486
619 619 c, t dbSNP:770882819
620 620 a, g dbSNP:185272164
642 642 a, g dbSNP:774103535
650 650 c, t dbSNP:16854294
660 660 c, g dbSNP:138429728
661 661 a, c dbSNP:771696873
668 668 c, g dbSNP:141160242
707 707 a, t dbSNP:547727221
714 714 c, g dbSNP:836703
734 734 c, t dbSNP:189004220
742 742 a, g dbSNP:768985890
745 745 c, t dbSNP:201625115
746 746 a, g dbSNP:367844597
751 751 c, g dbSNP:770163495
755 755 a, c dbSNP:146928065
759 759 g, t dbSNP:763518975
764 764 a, t dbSNP:771283993
767 767 a, g dbSNP:773969379
770 770 c, t dbSNP:538876129
771 771 a, g dbSNP:565639529
780 780 a, g dbSNP:767250099
788 788 c, g, t dbSNP:752421603
797 797 a, g dbSNP:763988418
810 810 a, g dbSNP:753632931
820 820 a, g dbSNP:757280673
821 821 g, t dbSNP:375631466
831 831 a, g dbSNP:778716438
834 834 a, c dbSNP:751705383
838 838 c, t dbSNP:558710710
841 841 g, t dbSNP:781202972
849 849 a, g dbSNP:748403364
856 856 a, t dbSNP:769938172
857 857 a, g dbSNP:368300022
864 864 a, g dbSNP:749603004
869 869 a, c dbSNP:372290869
870 870 -, ctt dbSNP:752133140
871 871 a, t dbSNP:139671055
872 872 a, c, t dbSNP:534924643
885 885 g, t dbSNP:771736989
886 886 c, t dbSNP:775071929
900 900 a, g dbSNP:760413775
904 904 c, t dbSNP:763822669
907 907 a, c dbSNP:776551627
914 914 a, g dbSNP:761486535
915 915 c, t dbSNP:117455823
925 925 c, t dbSNP:750353415
926 926 c, t dbSNP:758319224
929 929 a, g dbSNP:767590633
933 933 c, t dbSNP:752717358
942 942 c, t dbSNP:756386918
948 948 c, g dbSNP:199573451
955 955 c, t dbSNP:373371748
975 975 a, c dbSNP:757583316
976 976 a, t dbSNP:141683896
984 984 -, t dbSNP:759902933
986 986 a, c dbSNP:746364009
988 988 c, g, t dbSNP:376666492
989 989 a, g dbSNP:267598991
994 994 c, g dbSNP:746509718
997 997 c, t dbSNP:768458407
1006 1006 a, g dbSNP:776130624
1007 1007 a, g dbSNP:761481887
1011 1011 a, c, g dbSNP:145466790
1028 1028 a, g dbSNP:762893781
1030 1030 a, c dbSNP:766292044
1033 1033 c, t dbSNP:761945094
1042 1042 a, c, t dbSNP:765795611
1065 1065 a, g dbSNP:138238386
1083 1083 c, t dbSNP:764184986
1084 1084 c, g dbSNP:754037618
1086 1086 -, g dbSNP:767927493
1093 1093 c, t dbSNP:757431863
1108 1108 a, g dbSNP:779156495
1109 1109 a, g dbSNP:746228037
1110 1110 a, g dbSNP:759038173
1111 1111 -, g dbSNP:35087863
1112 1112 a, t dbSNP:780671699
1116 1116 a, g dbSNP:746571015
1126 1126 c, g, t dbSNP:768307081
1129 1129 a, g dbSNP:747763485
1135 1135 c, t dbSNP:142541144
1138 1138 a, g dbSNP:140091787
1154 1154 a, c dbSNP:762733734
1157 1157 a, g dbSNP:150305076
1172 1172 g, t dbSNP:774294184
1175 1175 a, g dbSNP:759429140
1177 1177 g, t dbSNP:764084910
1178 1178 c, g dbSNP:753895355
1183 1183 a, g dbSNP:142417537
1189 1189 a, g dbSNP:765490171
1198 1198 c, t dbSNP:115326727
1204 1204 c, t dbSNP:149194939
1208 1208 a, g dbSNP:758810298
1210 1210 a, g dbSNP:780522242
1213 1213 a, g dbSNP:369947361
1220 1220 a, c dbSNP:755478460
1221 1221 a, c, t dbSNP:373015124
1222 1222 c, t dbSNP:769401396
1233 1233 a, c dbSNP:750842338
1242 1242 c, g dbSNP:777452587
1255 1255 a, g dbSNP:144598275
1276 1276 c, t dbSNP:770743864
1277 1277 a, g dbSNP:373161902
1286 1286 g, t dbSNP:774065159
1287 1287 c, t dbSNP:759480593
1289 1289 a, g dbSNP:772030579
1293 1293 c, t dbSNP:776638733
1297 1297 a, g dbSNP:761917521
1299 1299 a, t dbSNP:765421546
1300 1300 c, t dbSNP:750700083
1318 1318 c, t dbSNP:763231079
1323 1323 -, t dbSNP:193920868
1337 1337 a, g dbSNP:150815941
1339 1339 c, t dbSNP:545323894
1346 1346 a, g dbSNP:145400335
1365 1365 c, t dbSNP:781720808
1366 1366 c, t dbSNP:752190776
1380 1380 a, g dbSNP:755628484
1399 1399 c, t dbSNP:777225229
1408 1408 c, t dbSNP:748943097
1411 1411 c, t dbSNP:35398738
1418 1418 c, t dbSNP:778736367
1420 1420 a, g dbSNP:569683849
1424 1424 a, c, t dbSNP:144099662
1433 1433 c, t dbSNP:746868901
1434 1434 a, g dbSNP:148250645
1495 1495 a, g dbSNP:773363253
1507 1507 a, c, t dbSNP:561399093
1511 1511 c, t dbSNP:774924741
1519 1519 c, t dbSNP:370442606
1520 1520 a, g dbSNP:767958450
1522 1522 c, t dbSNP:753270689
1525 1525 a, t dbSNP:756510877
1535 1535 a, g dbSNP:763607267
1544 1544 a, c dbSNP:753331247
1552 1552 c, g dbSNP:143825929
1555 1555 c, t dbSNP:778503573
1557 1557 a, c dbSNP:745694418
1558 1558 a, c dbSNP:758231595
1562 1562 c, t dbSNP:114283668
1563 1563 c, t dbSNP:746974862
1573 1573 c, t dbSNP:141741243
1579 1579 c, t dbSNP:150778768
1580 1580 a, g dbSNP:749376311
1582 1582 a, g dbSNP:771321632
1591 1591 a, g dbSNP:774693719
1601 1601 c, t dbSNP:759629237
1608 1608 g, t dbSNP:767929066
1613 1613 a, g dbSNP:775658885
1614 1614 -, ac dbSNP:752992100
1630 1630 c, g, t dbSNP:761300250
1666 1666 a, g, t dbSNP:372957873
1680 1680 a, g dbSNP:756735753
1686 1686 c, t dbSNP:764674116
1690 1690 c, t dbSNP:750117949
1693 1693 a, g dbSNP:551942376
1700 1700 a, g dbSNP:79263998
1705 1705 c, t dbSNP:113268857
1709 1709 a, g dbSNP:779917122
1716 1716 a, t dbSNP:751300954
1726 1726 c, g dbSNP:755036569
1731 1731 a, g dbSNP:781246280
1732 1732 a, t dbSNP:749429728
1756 1756 c, t dbSNP:368401399
1766 1766 a, g dbSNP:149529438
1769 1769 g, t dbSNP:746113953
1772 1772 c, t dbSNP:772171041
1773 1773 a, g dbSNP:775987464
1777 1777 a, g dbSNP:760913792
1778 1778 c, t dbSNP:836704
1781 1781 a, c dbSNP:752406848
1783 1783 a, c, g dbSNP:762264737
1801 1801 a, t dbSNP:749849142
1804 1804 -, aag dbSNP:756408320
1812 1812 a, g dbSNP:568000916
1828 1828 -, tat dbSNP:548543547
1833 1833 g, t dbSNP:536289475
1836 1836 a, g dbSNP:568314321
1843 1843 a, g dbSNP:575982254
1892 1892 a, g dbSNP:777362232
1894 1894 a, c dbSNP:12466817
1906 1906 c, t dbSNP:558749602

Target ORF information:

RefSeq Version XM_005246931
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005246931

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTTA G

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu11144D
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-1,3-galactosyltransferase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTTA G
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011510386.1
CDS530..1510
Misc Feature(1)803..1366(+)
Translation
Position Chain Variation Link
1 1 c, t dbSNP:546852279
5 5 a, g dbSNP:777530053
17 17 a, g dbSNP:566764950
24 24 a, g dbSNP:189283865
32 32 c, t dbSNP:180869998
33 33 a, g dbSNP:778038057
35 35 c, t dbSNP:142417380
54 54 a, g dbSNP:749625200
76 76 a, g dbSNP:568899703
106 106 a, g dbSNP:533716892
158 158 a, g dbSNP:79068432
162 162 a, g dbSNP:75814609
165 165 a, g dbSNP:114352176
185 185 a, g dbSNP:836722
202 202 c, t dbSNP:527551808
203 203 a, g dbSNP:836723
208 208 -, c dbSNP:750306272
222 222 -, ctc dbSNP:72249429
258 258 c, t dbSNP:773332197
261 261 c, t dbSNP:749338474
263 263 a, g dbSNP:13396351
286 286 a, c, t dbSNP:529424683
287 287 a, g dbSNP:549484614
300 300 a, g dbSNP:569501938
305 305 a, t dbSNP:552120919
315 315 c, t dbSNP:562616878
346 346 g, t dbSNP:777239799
355 355 g, t dbSNP:768161486
364 364 c, t dbSNP:770882819
365 365 a, g dbSNP:185272164
387 387 a, g dbSNP:774103535
395 395 c, t dbSNP:16854294
405 405 c, g dbSNP:138429728
406 406 a, c dbSNP:771696873
413 413 c, g dbSNP:141160242
452 452 a, t dbSNP:547727221
459 459 c, g dbSNP:836703
479 479 c, t dbSNP:189004220
487 487 a, g dbSNP:768985890
490 490 c, t dbSNP:201625115
491 491 a, g dbSNP:367844597
496 496 c, g dbSNP:770163495
500 500 a, c dbSNP:146928065
504 504 g, t dbSNP:763518975
509 509 a, t dbSNP:771283993
512 512 a, g dbSNP:773969379
515 515 c, t dbSNP:538876129
516 516 a, g dbSNP:565639529
525 525 a, g dbSNP:767250099
533 533 c, g, t dbSNP:752421603
542 542 a, g dbSNP:763988418
555 555 a, g dbSNP:753632931
565 565 a, g dbSNP:757280673
566 566 g, t dbSNP:375631466
576 576 a, g dbSNP:778716438
579 579 a, c dbSNP:751705383
583 583 c, t dbSNP:558710710
586 586 g, t dbSNP:781202972
594 594 a, g dbSNP:748403364
601 601 a, t dbSNP:769938172
602 602 a, g dbSNP:368300022
609 609 a, g dbSNP:749603004
614 614 a, c dbSNP:372290869
615 615 -, ctt dbSNP:752133140
616 616 a, t dbSNP:139671055
617 617 a, c, t dbSNP:534924643
630 630 g, t dbSNP:771736989
631 631 c, t dbSNP:775071929
645 645 a, g dbSNP:760413775
649 649 c, t dbSNP:763822669
652 652 a, c dbSNP:776551627
659 659 a, g dbSNP:761486535
660 660 c, t dbSNP:117455823
670 670 c, t dbSNP:750353415
671 671 c, t dbSNP:758319224
674 674 a, g dbSNP:767590633
678 678 c, t dbSNP:752717358
687 687 c, t dbSNP:756386918
693 693 c, g dbSNP:199573451
700 700 c, t dbSNP:373371748
720 720 a, c dbSNP:757583316
721 721 a, t dbSNP:141683896
729 729 -, t dbSNP:759902933
731 731 a, c dbSNP:746364009
733 733 c, g, t dbSNP:376666492
734 734 a, g dbSNP:267598991
739 739 c, g dbSNP:746509718
742 742 c, t dbSNP:768458407
751 751 a, g dbSNP:776130624
752 752 a, g dbSNP:761481887
756 756 a, c, g dbSNP:145466790
773 773 a, g dbSNP:762893781
775 775 a, c dbSNP:766292044
778 778 c, t dbSNP:761945094
787 787 a, c, t dbSNP:765795611
810 810 a, g dbSNP:138238386
828 828 c, t dbSNP:764184986
829 829 c, g dbSNP:754037618
831 831 -, g dbSNP:767927493
838 838 c, t dbSNP:757431863
853 853 a, g dbSNP:779156495
854 854 a, g dbSNP:746228037
855 855 a, g dbSNP:759038173
856 856 -, g dbSNP:35087863
857 857 a, t dbSNP:780671699
861 861 a, g dbSNP:746571015
871 871 c, g, t dbSNP:768307081
874 874 a, g dbSNP:747763485
880 880 c, t dbSNP:142541144
883 883 a, g dbSNP:140091787
899 899 a, c dbSNP:762733734
902 902 a, g dbSNP:150305076
917 917 g, t dbSNP:774294184
920 920 a, g dbSNP:759429140
922 922 g, t dbSNP:764084910
923 923 c, g dbSNP:753895355
928 928 a, g dbSNP:142417537
934 934 a, g dbSNP:765490171
943 943 c, t dbSNP:115326727
949 949 c, t dbSNP:149194939
953 953 a, g dbSNP:758810298
955 955 a, g dbSNP:780522242
958 958 a, g dbSNP:369947361
965 965 a, c dbSNP:755478460
966 966 a, c, t dbSNP:373015124
967 967 c, t dbSNP:769401396
978 978 a, c dbSNP:750842338
987 987 c, g dbSNP:777452587
1000 1000 a, g dbSNP:144598275
1021 1021 c, t dbSNP:770743864
1022 1022 a, g dbSNP:373161902
1031 1031 g, t dbSNP:774065159
1032 1032 c, t dbSNP:759480593
1034 1034 a, g dbSNP:772030579
1038 1038 c, t dbSNP:776638733
1042 1042 a, g dbSNP:761917521
1044 1044 a, t dbSNP:765421546
1045 1045 c, t dbSNP:750700083
1063 1063 c, t dbSNP:763231079
1068 1068 -, t dbSNP:193920868
1082 1082 a, g dbSNP:150815941
1084 1084 c, t dbSNP:545323894
1091 1091 a, g dbSNP:145400335
1110 1110 c, t dbSNP:781720808
1111 1111 c, t dbSNP:752190776
1125 1125 a, g dbSNP:755628484
1144 1144 c, t dbSNP:777225229
1153 1153 c, t dbSNP:748943097
1156 1156 c, t dbSNP:35398738
1163 1163 c, t dbSNP:778736367
1165 1165 a, g dbSNP:569683849
1169 1169 a, c, t dbSNP:144099662
1178 1178 c, t dbSNP:746868901
1179 1179 a, g dbSNP:148250645
1240 1240 a, g dbSNP:773363253
1252 1252 a, c, t dbSNP:561399093
1256 1256 c, t dbSNP:774924741
1264 1264 c, t dbSNP:370442606
1265 1265 a, g dbSNP:767958450
1267 1267 c, t dbSNP:753270689
1270 1270 a, t dbSNP:756510877
1280 1280 a, g dbSNP:763607267
1289 1289 a, c dbSNP:753331247
1297 1297 c, g dbSNP:143825929
1300 1300 c, t dbSNP:778503573
1302 1302 a, c dbSNP:745694418
1303 1303 a, c dbSNP:758231595
1307 1307 c, t dbSNP:114283668
1308 1308 c, t dbSNP:746974862
1318 1318 c, t dbSNP:141741243
1324 1324 c, t dbSNP:150778768
1325 1325 a, g dbSNP:749376311
1327 1327 a, g dbSNP:771321632
1336 1336 a, g dbSNP:774693719
1346 1346 c, t dbSNP:759629237
1353 1353 g, t dbSNP:767929066
1358 1358 a, g dbSNP:775658885
1359 1359 -, ac dbSNP:752992100
1375 1375 c, g, t dbSNP:761300250
1411 1411 a, g, t dbSNP:372957873
1425 1425 a, g dbSNP:756735753
1431 1431 c, t dbSNP:764674116
1435 1435 c, t dbSNP:750117949
1438 1438 a, g dbSNP:551942376
1445 1445 a, g dbSNP:79263998
1450 1450 c, t dbSNP:113268857
1454 1454 a, g dbSNP:779917122
1461 1461 a, t dbSNP:751300954
1471 1471 c, g dbSNP:755036569
1476 1476 a, g dbSNP:781246280
1477 1477 a, t dbSNP:749429728
1501 1501 c, t dbSNP:368401399
1511 1511 a, g dbSNP:149529438
1514 1514 g, t dbSNP:746113953
1517 1517 c, t dbSNP:772171041
1518 1518 a, g dbSNP:775987464
1522 1522 a, g dbSNP:760913792
1523 1523 c, t dbSNP:836704
1526 1526 a, c dbSNP:752406848
1528 1528 a, c, g dbSNP:762264737
1546 1546 a, t dbSNP:749849142
1549 1549 -, aag dbSNP:756408320
1557 1557 a, g dbSNP:568000916
1573 1573 -, tat dbSNP:548543547
1578 1578 g, t dbSNP:536289475
1581 1581 a, g dbSNP:568314321
1588 1588 a, g dbSNP:575982254
1637 1637 a, g dbSNP:777362232
1639 1639 a, c dbSNP:12466817
1651 1651 c, t dbSNP:558749602

Target ORF information:

RefSeq Version XM_011512084
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011512084

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTTA G

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu11144D
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-1,3-galactosyltransferase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578805076. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTTA G
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_006712882.1
CDS548..1528
Misc Feature(1)821..1384(+)
Translation
Position Chain Variation Link
35 35 a, g dbSNP:745710287
49 49 a, g dbSNP:62195004
88 88 a, t dbSNP:757356845
103 103 c, t dbSNP:566174319
108 108 a, t dbSNP:376686092
120 120 a, t dbSNP:548770223
130 130 a, g dbSNP:780074309
132 132 a, t dbSNP:568772449
135 135 a, g dbSNP:537699171
145 145 a, g dbSNP:556722658
182 182 a, g dbSNP:375042060
203 203 a, g dbSNP:836722
220 220 c, t dbSNP:527551808
221 221 a, g dbSNP:836723
226 226 -, c dbSNP:750306272
240 240 -, ctc dbSNP:72249429
276 276 c, t dbSNP:773332197
279 279 c, t dbSNP:749338474
281 281 a, g dbSNP:13396351
304 304 a, c, t dbSNP:529424683
305 305 a, g dbSNP:549484614
318 318 a, g dbSNP:569501938
323 323 a, t dbSNP:552120919
333 333 c, t dbSNP:562616878
364 364 g, t dbSNP:777239799
373 373 g, t dbSNP:768161486
382 382 c, t dbSNP:770882819
383 383 a, g dbSNP:185272164
405 405 a, g dbSNP:774103535
413 413 c, t dbSNP:16854294
423 423 c, g dbSNP:138429728
424 424 a, c dbSNP:771696873
431 431 c, g dbSNP:141160242
470 470 a, t dbSNP:547727221
477 477 c, g dbSNP:836703
497 497 c, t dbSNP:189004220
505 505 a, g dbSNP:768985890
508 508 c, t dbSNP:201625115
509 509 a, g dbSNP:367844597
514 514 c, g dbSNP:770163495
518 518 a, c dbSNP:146928065
522 522 g, t dbSNP:763518975
527 527 a, t dbSNP:771283993
530 530 a, g dbSNP:773969379
533 533 c, t dbSNP:538876129
534 534 a, g dbSNP:565639529
543 543 a, g dbSNP:767250099
551 551 c, g, t dbSNP:752421603
560 560 a, g dbSNP:763988418
573 573 a, g dbSNP:753632931
583 583 a, g dbSNP:757280673
584 584 g, t dbSNP:375631466
594 594 a, g dbSNP:778716438
597 597 a, c dbSNP:751705383
601 601 c, t dbSNP:558710710
604 604 g, t dbSNP:781202972
612 612 a, g dbSNP:748403364
619 619 a, t dbSNP:769938172
620 620 a, g dbSNP:368300022
627 627 a, g dbSNP:749603004
632 632 a, c dbSNP:372290869
633 633 -, ctt dbSNP:752133140
634 634 a, t dbSNP:139671055
635 635 a, c, t dbSNP:534924643
648 648 g, t dbSNP:771736989
649 649 c, t dbSNP:775071929
663 663 a, g dbSNP:760413775
667 667 c, t dbSNP:763822669
670 670 a, c dbSNP:776551627
677 677 a, g dbSNP:761486535
678 678 c, t dbSNP:117455823
688 688 c, t dbSNP:750353415
689 689 c, t dbSNP:758319224
692 692 a, g dbSNP:767590633
696 696 c, t dbSNP:752717358
705 705 c, t dbSNP:756386918
711 711 c, g dbSNP:199573451
718 718 c, t dbSNP:373371748
738 738 a, c dbSNP:757583316
739 739 a, t dbSNP:141683896
747 747 -, t dbSNP:759902933
749 749 a, c dbSNP:746364009
751 751 c, g, t dbSNP:376666492
752 752 a, g dbSNP:267598991
757 757 c, g dbSNP:746509718
760 760 c, t dbSNP:768458407
769 769 a, g dbSNP:776130624
770 770 a, g dbSNP:761481887
774 774 a, c, g dbSNP:145466790
791 791 a, g dbSNP:762893781
793 793 a, c dbSNP:766292044
796 796 c, t dbSNP:761945094
805 805 a, c, t dbSNP:765795611
828 828 a, g dbSNP:138238386
846 846 c, t dbSNP:764184986
847 847 c, g dbSNP:754037618
849 849 -, g dbSNP:767927493
856 856 c, t dbSNP:757431863
871 871 a, g dbSNP:779156495
872 872 a, g dbSNP:746228037
873 873 a, g dbSNP:759038173
874 874 -, g dbSNP:35087863
875 875 a, t dbSNP:780671699
879 879 a, g dbSNP:746571015
889 889 c, g, t dbSNP:768307081
892 892 a, g dbSNP:747763485
898 898 c, t dbSNP:142541144
901 901 a, g dbSNP:140091787
917 917 a, c dbSNP:762733734
920 920 a, g dbSNP:150305076
935 935 g, t dbSNP:774294184
938 938 a, g dbSNP:759429140
940 940 g, t dbSNP:764084910
941 941 c, g dbSNP:753895355
946 946 a, g dbSNP:142417537
952 952 a, g dbSNP:765490171
961 961 c, t dbSNP:115326727
967 967 c, t dbSNP:149194939
971 971 a, g dbSNP:758810298
973 973 a, g dbSNP:780522242
976 976 a, g dbSNP:369947361
983 983 a, c dbSNP:755478460
984 984 a, c, t dbSNP:373015124
985 985 c, t dbSNP:769401396
996 996 a, c dbSNP:750842338
1005 1005 c, g dbSNP:777452587
1018 1018 a, g dbSNP:144598275
1039 1039 c, t dbSNP:770743864
1040 1040 a, g dbSNP:373161902
1049 1049 g, t dbSNP:774065159
1050 1050 c, t dbSNP:759480593
1052 1052 a, g dbSNP:772030579
1056 1056 c, t dbSNP:776638733
1060 1060 a, g dbSNP:761917521
1062 1062 a, t dbSNP:765421546
1063 1063 c, t dbSNP:750700083
1081 1081 c, t dbSNP:763231079
1086 1086 -, t dbSNP:193920868
1100 1100 a, g dbSNP:150815941
1102 1102 c, t dbSNP:545323894
1109 1109 a, g dbSNP:145400335
1128 1128 c, t dbSNP:781720808
1129 1129 c, t dbSNP:752190776
1143 1143 a, g dbSNP:755628484
1162 1162 c, t dbSNP:777225229
1171 1171 c, t dbSNP:748943097
1174 1174 c, t dbSNP:35398738
1181 1181 c, t dbSNP:778736367
1183 1183 a, g dbSNP:569683849
1187 1187 a, c, t dbSNP:144099662
1196 1196 c, t dbSNP:746868901
1197 1197 a, g dbSNP:148250645
1258 1258 a, g dbSNP:773363253
1270 1270 a, c, t dbSNP:561399093
1274 1274 c, t dbSNP:774924741
1282 1282 c, t dbSNP:370442606
1283 1283 a, g dbSNP:767958450
1285 1285 c, t dbSNP:753270689
1288 1288 a, t dbSNP:756510877
1298 1298 a, g dbSNP:763607267
1307 1307 a, c dbSNP:753331247
1315 1315 c, g dbSNP:143825929
1318 1318 c, t dbSNP:778503573
1320 1320 a, c dbSNP:745694418
1321 1321 a, c dbSNP:758231595
1325 1325 c, t dbSNP:114283668
1326 1326 c, t dbSNP:746974862
1336 1336 c, t dbSNP:141741243
1342 1342 c, t dbSNP:150778768
1343 1343 a, g dbSNP:749376311
1345 1345 a, g dbSNP:771321632
1354 1354 a, g dbSNP:774693719
1364 1364 c, t dbSNP:759629237
1371 1371 g, t dbSNP:767929066
1376 1376 a, g dbSNP:775658885
1377 1377 -, ac dbSNP:752992100
1393 1393 c, g, t dbSNP:761300250
1429 1429 a, g, t dbSNP:372957873
1443 1443 a, g dbSNP:756735753
1449 1449 c, t dbSNP:764674116
1453 1453 c, t dbSNP:750117949
1456 1456 a, g dbSNP:551942376
1463 1463 a, g dbSNP:79263998
1468 1468 c, t dbSNP:113268857
1472 1472 a, g dbSNP:779917122
1479 1479 a, t dbSNP:751300954
1489 1489 c, g dbSNP:755036569
1494 1494 a, g dbSNP:781246280
1495 1495 a, t dbSNP:749429728
1519 1519 c, t dbSNP:368401399
1529 1529 a, g dbSNP:149529438
1532 1532 g, t dbSNP:746113953
1535 1535 c, t dbSNP:772171041
1536 1536 a, g dbSNP:775987464
1540 1540 a, g dbSNP:760913792
1541 1541 c, t dbSNP:836704
1544 1544 a, c dbSNP:752406848
1546 1546 a, c, g dbSNP:762264737
1564 1564 a, t dbSNP:749849142
1567 1567 -, aag dbSNP:756408320
1575 1575 a, g dbSNP:568000916
1591 1591 -, tat dbSNP:548543547
1596 1596 g, t dbSNP:536289475
1599 1599 a, g dbSNP:568314321
1606 1606 a, g dbSNP:575982254
1655 1655 a, g dbSNP:777362232
1657 1657 a, c dbSNP:12466817
1669 1669 c, t dbSNP:558749602

Target ORF information:

RefSeq Version XM_006712819
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_006712819

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTTA G

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu11144D
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product beta-1,3-galactosyltransferase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTTA G
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011510387.1
CDS801..1781
Misc Feature(1)1074..1637(+)
Translation
Position Chain Variation Link
1 1 c, g dbSNP:140101666
3 3 a, g dbSNP:527637881
30 30 g, t dbSNP:111275619
45 45 c, t dbSNP:546965326
84 84 g, t dbSNP:566784392
89 89 g, t dbSNP:535927288
102 102 a, g dbSNP:549468288
118 118 a, g dbSNP:569387320
124 124 a, g dbSNP:538399879
196 196 c, t dbSNP:558071059
205 205 c, g dbSNP:143685188
216 216 c, t dbSNP:578193770
260 260 c, g dbSNP:529493033
272 272 c, t dbSNP:373675441
279 279 a, g dbSNP:189283865
287 287 c, t dbSNP:180869998
288 288 a, g dbSNP:778038057
290 290 c, t dbSNP:142417380
309 309 a, g dbSNP:749625200
331 331 a, g dbSNP:568899703
361 361 a, g dbSNP:533716892
413 413 a, g dbSNP:79068432
417 417 a, g dbSNP:75814609
420 420 a, g dbSNP:114352176
440 440 a, g dbSNP:836722
457 457 c, t dbSNP:527551808
458 458 a, g dbSNP:836723
463 463 -, c dbSNP:750306272
477 477 -, ctc dbSNP:72249429
513 513 c, t dbSNP:773332197
516 516 c, t dbSNP:749338474
518 518 a, g dbSNP:13396351
541 541 a, c, t dbSNP:529424683
542 542 a, g dbSNP:549484614
555 555 a, g dbSNP:569501938
557 557 a, t dbSNP:759898959
576 576 a, t dbSNP:552120919
586 586 c, t dbSNP:562616878
617 617 g, t dbSNP:777239799
626 626 g, t dbSNP:768161486
635 635 c, t dbSNP:770882819
636 636 a, g dbSNP:185272164
658 658 a, g dbSNP:774103535
666 666 c, t dbSNP:16854294
676 676 c, g dbSNP:138429728
677 677 a, c dbSNP:771696873
684 684 c, g dbSNP:141160242
723 723 a, t dbSNP:547727221
730 730 c, g dbSNP:836703
750 750 c, t dbSNP:189004220
758 758 a, g dbSNP:768985890
761 761 c, t dbSNP:201625115
762 762 a, g dbSNP:367844597
767 767 c, g dbSNP:770163495
771 771 a, c dbSNP:146928065
775 775 g, t dbSNP:763518975
780 780 a, t dbSNP:771283993
783 783 a, g dbSNP:773969379
786 786 c, t dbSNP:538876129
787 787 a, g dbSNP:565639529
796 796 a, g dbSNP:767250099
804 804 c, g, t dbSNP:752421603
813 813 a, g dbSNP:763988418
826 826 a, g dbSNP:753632931
836 836 a, g dbSNP:757280673
837 837 g, t dbSNP:375631466
847 847 a, g dbSNP:778716438
850 850 a, c dbSNP:751705383
854 854 c, t dbSNP:558710710
857 857 g, t dbSNP:781202972
865 865 a, g dbSNP:748403364
872 872 a, t dbSNP:769938172
873 873 a, g dbSNP:368300022
880 880 a, g dbSNP:749603004
885 885 a, c dbSNP:372290869
886 886 -, ctt dbSNP:752133140
887 887 a, t dbSNP:139671055
888 888 a, c, t dbSNP:534924643
901 901 g, t dbSNP:771736989
902 902 c, t dbSNP:775071929
916 916 a, g dbSNP:760413775
920 920 c, t dbSNP:763822669
923 923 a, c dbSNP:776551627
930 930 a, g dbSNP:761486535
931 931 c, t dbSNP:117455823
941 941 c, t dbSNP:750353415
942 942 c, t dbSNP:758319224
945 945 a, g dbSNP:767590633
949 949 c, t dbSNP:752717358
958 958 c, t dbSNP:756386918
964 964 c, g dbSNP:199573451
971 971 c, t dbSNP:373371748
991 991 a, c dbSNP:757583316
992 992 a, t dbSNP:141683896
1000 1000 -, t dbSNP:759902933
1002 1002 a, c dbSNP:746364009
1004 1004 c, g, t dbSNP:376666492
1005 1005 a, g dbSNP:267598991
1010 1010 c, g dbSNP:746509718
1013 1013 c, t dbSNP:768458407
1022 1022 a, g dbSNP:776130624
1023 1023 a, g dbSNP:761481887
1027 1027 a, c, g dbSNP:145466790
1044 1044 a, g dbSNP:762893781
1046 1046 a, c dbSNP:766292044
1049 1049 c, t dbSNP:761945094
1058 1058 a, c, t dbSNP:765795611
1081 1081 a, g dbSNP:138238386
1099 1099 c, t dbSNP:764184986
1100 1100 c, g dbSNP:754037618
1102 1102 -, g dbSNP:767927493
1109 1109 c, t dbSNP:757431863
1124 1124 a, g dbSNP:779156495
1125 1125 a, g dbSNP:746228037
1126 1126 a, g dbSNP:759038173
1127 1127 -, g dbSNP:35087863
1128 1128 a, t dbSNP:780671699
1132 1132 a, g dbSNP:746571015
1142 1142 c, g, t dbSNP:768307081
1145 1145 a, g dbSNP:747763485
1151 1151 c, t dbSNP:142541144
1154 1154 a, g dbSNP:140091787
1170 1170 a, c dbSNP:762733734
1173 1173 a, g dbSNP:150305076
1188 1188 g, t dbSNP:774294184
1191 1191 a, g dbSNP:759429140
1193 1193 g, t dbSNP:764084910
1194 1194 c, g dbSNP:753895355
1199 1199 a, g dbSNP:142417537
1205 1205 a, g dbSNP:765490171
1214 1214 c, t dbSNP:115326727
1220 1220 c, t dbSNP:149194939
1224 1224 a, g dbSNP:758810298
1226 1226 a, g dbSNP:780522242
1229 1229 a, g dbSNP:369947361
1236 1236 a, c dbSNP:755478460
1237 1237 a, c, t dbSNP:373015124
1238 1238 c, t dbSNP:769401396
1249 1249 a, c dbSNP:750842338
1258 1258 c, g dbSNP:777452587
1271 1271 a, g dbSNP:144598275
1292 1292 c, t dbSNP:770743864
1293 1293 a, g dbSNP:373161902
1302 1302 g, t dbSNP:774065159
1303 1303 c, t dbSNP:759480593
1305 1305 a, g dbSNP:772030579
1309 1309 c, t dbSNP:776638733
1313 1313 a, g dbSNP:761917521
1315 1315 a, t dbSNP:765421546
1316 1316 c, t dbSNP:750700083
1334 1334 c, t dbSNP:763231079
1339 1339 -, t dbSNP:193920868
1353 1353 a, g dbSNP:150815941
1355 1355 c, t dbSNP:545323894
1362 1362 a, g dbSNP:145400335
1381 1381 c, t dbSNP:781720808
1382 1382 c, t dbSNP:752190776
1396 1396 a, g dbSNP:755628484
1415 1415 c, t dbSNP:777225229
1424 1424 c, t dbSNP:748943097
1427 1427 c, t dbSNP:35398738
1434 1434 c, t dbSNP:778736367
1436 1436 a, g dbSNP:569683849
1440 1440 a, c, t dbSNP:144099662
1449 1449 c, t dbSNP:746868901
1450 1450 a, g dbSNP:148250645
1511 1511 a, g dbSNP:773363253
1523 1523 a, c, t dbSNP:561399093
1527 1527 c, t dbSNP:774924741
1535 1535 c, t dbSNP:370442606
1536 1536 a, g dbSNP:767958450
1538 1538 c, t dbSNP:753270689
1541 1541 a, t dbSNP:756510877
1551 1551 a, g dbSNP:763607267
1560 1560 a, c dbSNP:753331247
1568 1568 c, g dbSNP:143825929
1571 1571 c, t dbSNP:778503573
1573 1573 a, c dbSNP:745694418
1574 1574 a, c dbSNP:758231595
1578 1578 c, t dbSNP:114283668
1579 1579 c, t dbSNP:746974862
1589 1589 c, t dbSNP:141741243
1595 1595 c, t dbSNP:150778768
1596 1596 a, g dbSNP:749376311
1598 1598 a, g dbSNP:771321632
1607 1607 a, g dbSNP:774693719
1617 1617 c, t dbSNP:759629237
1624 1624 g, t dbSNP:767929066
1629 1629 a, g dbSNP:775658885
1630 1630 -, ac dbSNP:752992100
1646 1646 c, g, t dbSNP:761300250
1682 1682 a, g, t dbSNP:372957873
1696 1696 a, g dbSNP:756735753
1702 1702 c, t dbSNP:764674116
1706 1706 c, t dbSNP:750117949
1709 1709 a, g dbSNP:551942376
1716 1716 a, g dbSNP:79263998
1721 1721 c, t dbSNP:113268857
1725 1725 a, g dbSNP:779917122
1732 1732 a, t dbSNP:751300954
1742 1742 c, g dbSNP:755036569
1747 1747 a, g dbSNP:781246280
1748 1748 a, t dbSNP:749429728
1772 1772 c, t dbSNP:368401399
1782 1782 a, g dbSNP:149529438
1785 1785 g, t dbSNP:746113953
1788 1788 c, t dbSNP:772171041
1789 1789 a, g dbSNP:775987464
1793 1793 a, g dbSNP:760913792
1794 1794 c, t dbSNP:836704
1797 1797 a, c dbSNP:752406848
1799 1799 a, c, g dbSNP:762264737
1817 1817 a, t dbSNP:749849142
1820 1820 -, aag dbSNP:756408320
1828 1828 a, g dbSNP:568000916
1844 1844 -, tat dbSNP:548543547
1849 1849 g, t dbSNP:536289475
1852 1852 a, g dbSNP:568314321
1859 1859 a, g dbSNP:575982254
1908 1908 a, g dbSNP:777362232
1910 1910 a, c dbSNP:12466817
1922 1922 c, t dbSNP:558749602

Target ORF information:

RefSeq Version XM_011512085
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011512085

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTTA G

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu11144D
Sequence Information ORF Nucleotide Sequence (Length: 981bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product beta-1,3-galactosyltransferase 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC016723.11 and AF117222.1. On Sep 3, 2010 this sequence version replaced gi:15451870. Summary: This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is expressed exclusively in the brain. The encoded protein shows strict donor substrate specificity for UDP-galactose. [provided by RefSeq, Jul 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: AF117222.1, BC101545.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTTA G
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_066191.1
CDS352..1332
Misc Feature(1)298..300(+)
Misc Feature(2)370..429(+)
Misc Feature(3)625..1188(+)
Exon (1)1..122
Gene:B3GALT1
Gene Synonym:
Exon (2)123..2168
Gene:B3GALT1
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, g dbSNP:836722
24 24 c, t dbSNP:527551808
25 25 a, g dbSNP:836723
30 30 -, c dbSNP:750306272
44 44 -, ctc dbSNP:72249429
80 80 c, t dbSNP:773332197
83 83 c, t dbSNP:749338474
85 85 a, g dbSNP:13396351
108 108 a, c, t dbSNP:529424683
109 109 a, g dbSNP:549484614
122 122 a, g dbSNP:569501938
127 127 a, t dbSNP:552120919
137 137 c, t dbSNP:562616878
168 168 g, t dbSNP:777239799
177 177 g, t dbSNP:768161486
186 186 c, t dbSNP:770882819
187 187 a, g dbSNP:185272164
209 209 a, g dbSNP:774103535
217 217 c, t dbSNP:16854294
227 227 c, g dbSNP:138429728
228 228 a, c dbSNP:771696873
235 235 c, g dbSNP:141160242
274 274 a, t dbSNP:547727221
281 281 c, g dbSNP:836703
301 301 c, t dbSNP:189004220
309 309 a, g dbSNP:768985890
312 312 c, t dbSNP:201625115
313 313 a, g dbSNP:367844597
318 318 c, g dbSNP:770163495
322 322 a, c dbSNP:146928065
326 326 g, t dbSNP:763518975
331 331 a, t dbSNP:771283993
334 334 a, g dbSNP:773969379
337 337 c, t dbSNP:538876129
338 338 a, g dbSNP:565639529
347 347 a, g dbSNP:767250099
355 355 c, g, t dbSNP:752421603
364 364 a, g dbSNP:763988418
377 377 a, g dbSNP:753632931
387 387 a, g dbSNP:757280673
388 388 g, t dbSNP:375631466
398 398 a, g dbSNP:778716438
401 401 a, c dbSNP:751705383
405 405 c, t dbSNP:558710710
408 408 g, t dbSNP:781202972
416 416 a, g dbSNP:748403364
423 423 a, t dbSNP:769938172
424 424 a, g dbSNP:368300022
431 431 a, g dbSNP:749603004
436 436 a, c dbSNP:372290869
437 437 -, ctt dbSNP:752133140
438 438 a, t dbSNP:139671055
439 439 a, c, t dbSNP:534924643
452 452 g, t dbSNP:771736989
453 453 c, t dbSNP:775071929
467 467 a, g dbSNP:760413775
471 471 c, t dbSNP:763822669
474 474 a, c dbSNP:776551627
481 481 a, g dbSNP:761486535
482 482 c, t dbSNP:117455823
492 492 c, t dbSNP:750353415
493 493 c, t dbSNP:758319224
496 496 a, g dbSNP:767590633
500 500 c, t dbSNP:752717358
509 509 c, t dbSNP:756386918
515 515 c, g dbSNP:199573451
522 522 c, t dbSNP:373371748
542 542 a, c dbSNP:757583316
543 543 a, t dbSNP:141683896
551 551 -, t dbSNP:759902933
553 553 a, c dbSNP:746364009
555 555 c, g, t dbSNP:376666492
556 556 a, g dbSNP:267598991
561 561 c, g dbSNP:746509718
564 564 c, t dbSNP:768458407
573 573 a, g dbSNP:776130624
574 574 a, g dbSNP:761481887
578 578 a, c, g dbSNP:145466790
595 595 a, g dbSNP:762893781
597 597 a, c dbSNP:766292044
600 600 c, t dbSNP:761945094
609 609 a, c, t dbSNP:765795611
632 632 a, g dbSNP:138238386
650 650 c, t dbSNP:764184986
651 651 c, g dbSNP:754037618
653 653 -, g dbSNP:767927493
660 660 c, t dbSNP:757431863
675 675 a, g dbSNP:779156495
676 676 a, g dbSNP:746228037
677 677 a, g dbSNP:759038173
678 678 -, g dbSNP:35087863
679 679 a, t dbSNP:780671699
683 683 a, g dbSNP:746571015
693 693 c, g, t dbSNP:768307081
696 696 a, g dbSNP:747763485
702 702 c, t dbSNP:142541144
705 705 a, g dbSNP:140091787
721 721 a, c dbSNP:762733734
724 724 a, g dbSNP:150305076
739 739 g, t dbSNP:774294184
742 742 a, g dbSNP:759429140
744 744 g, t dbSNP:764084910
745 745 c, g dbSNP:753895355
750 750 a, g dbSNP:142417537
756 756 a, g dbSNP:765490171
765 765 c, t dbSNP:115326727
771 771 c, t dbSNP:149194939
775 775 a, g dbSNP:758810298
777 777 a, g dbSNP:780522242
780 780 a, g dbSNP:369947361
787 787 a, c dbSNP:755478460
788 788 a, c, t dbSNP:373015124
789 789 c, t dbSNP:769401396
800 800 a, c dbSNP:750842338
809 809 c, g dbSNP:777452587
822 822 a, g dbSNP:144598275
843 843 c, t dbSNP:770743864
844 844 a, g dbSNP:373161902
853 853 g, t dbSNP:774065159
854 854 c, t dbSNP:759480593
856 856 a, g dbSNP:772030579
860 860 c, t dbSNP:776638733
864 864 a, g dbSNP:761917521
866 866 a, t dbSNP:765421546
867 867 c, t dbSNP:750700083
885 885 c, t dbSNP:763231079
890 890 -, t dbSNP:193920868
904 904 a, g dbSNP:150815941
906 906 c, t dbSNP:545323894
913 913 a, g dbSNP:145400335
932 932 c, t dbSNP:781720808
933 933 c, t dbSNP:752190776
947 947 a, g dbSNP:755628484
966 966 c, t dbSNP:777225229
975 975 c, t dbSNP:748943097
978 978 c, t dbSNP:35398738
985 985 c, t dbSNP:778736367
987 987 a, g dbSNP:569683849
991 991 a, c, t dbSNP:144099662
1000 1000 c, t dbSNP:746868901
1001 1001 a, g dbSNP:148250645
1062 1062 a, g dbSNP:773363253
1074 1074 a, c, t dbSNP:561399093
1078 1078 c, t dbSNP:774924741
1086 1086 c, t dbSNP:370442606
1087 1087 a, g dbSNP:767958450
1089 1089 c, t dbSNP:753270689
1092 1092 a, t dbSNP:756510877
1102 1102 a, g dbSNP:763607267
1111 1111 a, c dbSNP:753331247
1119 1119 c, g dbSNP:143825929
1122 1122 c, t dbSNP:778503573
1124 1124 a, c dbSNP:745694418
1125 1125 a, c dbSNP:758231595
1129 1129 c, t dbSNP:114283668
1130 1130 c, t dbSNP:746974862
1140 1140 c, t dbSNP:141741243
1146 1146 c, t dbSNP:150778768
1147 1147 a, g dbSNP:749376311
1149 1149 a, g dbSNP:771321632
1158 1158 a, g dbSNP:774693719
1168 1168 c, t dbSNP:759629237
1175 1175 g, t dbSNP:767929066
1180 1180 a, g dbSNP:775658885
1181 1181 -, ac dbSNP:752992100
1197 1197 c, g, t dbSNP:761300250
1233 1233 a, g, t dbSNP:372957873
1247 1247 a, g dbSNP:756735753
1253 1253 c, t dbSNP:764674116
1257 1257 c, t dbSNP:750117949
1260 1260 a, g dbSNP:551942376
1267 1267 a, g dbSNP:79263998
1272 1272 c, t dbSNP:113268857
1276 1276 a, g dbSNP:779917122
1283 1283 a, t dbSNP:751300954
1293 1293 c, g dbSNP:755036569
1298 1298 a, g dbSNP:781246280
1299 1299 a, t dbSNP:749429728
1323 1323 c, t dbSNP:368401399
1333 1333 a, g dbSNP:149529438
1336 1336 g, t dbSNP:746113953
1339 1339 c, t dbSNP:772171041
1340 1340 a, g dbSNP:775987464
1344 1344 a, g dbSNP:760913792
1345 1345 c, t dbSNP:836704
1348 1348 a, c dbSNP:752406848
1350 1350 a, c, g dbSNP:762264737
1368 1368 a, t dbSNP:749849142
1371 1371 -, aag dbSNP:756408320
1379 1379 a, g dbSNP:568000916
1395 1395 -, tat dbSNP:548543547
1400 1400 g, t dbSNP:536289475
1403 1403 a, g dbSNP:568314321
1410 1410 a, g dbSNP:575982254
1459 1459 a, g dbSNP:777362232
1461 1461 a, c dbSNP:12466817
1473 1473 c, t dbSNP:558749602
1580 1580 c, g dbSNP:756659945
1589 1589 a, t dbSNP:778913264
1590 1590 a, g dbSNP:751527173
1609 1609 a, g dbSNP:572623194
1612 1612 c, t dbSNP:541248218
1614 1614 a, g dbSNP:187063252
1658 1658 a, g dbSNP:765839153
1673 1673 c, g dbSNP:769032248
1677 1677 c, t dbSNP:141242987
1678 1678 -, c dbSNP:35838143
1706 1706 a, c, t dbSNP:192347044
1772 1772 c, t dbSNP:78477111
1773 1773 a, g dbSNP:754894600
1777 1777 a, g dbSNP:150648556
1778 1778 g, t dbSNP:769700446
1787 1787 a, g dbSNP:773838578
1866 1866 c, g dbSNP:546491959
1892 1892 a, t dbSNP:763359826
1919 1919 g, t dbSNP:766804459
1932 1932 a, c dbSNP:552270148
1949 1949 a, g dbSNP:77238840
1968 1968 c, t dbSNP:528324940
1998 1998 c, t dbSNP:548076141
2014 2014 -, t dbSNP:150091257
2021 2021 -, tttc dbSNP:200098380
2022 2022 -, ttc dbSNP:201261960
2024 2024 -, c dbSNP:752881445
2025 2025 -, t dbSNP:574966725
2028 2028 c, t dbSNP:568063370
2031 2031 -, tttc dbSNP:759797003
2034 2034 c, t dbSNP:536951112
2046 2046 g, t dbSNP:2054368
2132 2132 c, t dbSNP:756232022
2133 2133 a, g dbSNP:369371514
2139 2139 g, t dbSNP:183349062
2155 2155 c, t dbSNP:569732016
2157 2157 a, g dbSNP:759840415
2161 2161 c, t dbSNP:374818265

Target ORF information:

RefSeq Version NM_020981
Organism Homo sapiens (human)
Definition Homo sapiens UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 (B3GALT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_020981

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTTCAA AGGTCTCCTG TTTGTATGTT TTGACAGTTG TGTGCTGGGC CAGCGCTCTC 
TGGTACTTGA GTATAACTCG CCCTACTTCT TCTTACACTG GCTCCAAACC ATTCAGCCAC
CTAACAGTTG CCAGGAAAAA CTTCACCTTT GGCAACATAA GAACTCGACC TATCAACCCA
CATTCTTTTG AATTTCTTAT CAACGAGCCC AATAAATGTG AGAAAAACAT TCCTTTTCTT
GTTATCCTCA TCAGCACCAC TCACAAGGAA TTTGATGCCC GTCAGGCAAT CAGAGAGACG
TGGGGGGATG AGAACAACTT TAAGGGGATC AAGATAGCCA CCCTGTTCCT CCTGGGCAAG
AATGCTGATC CTGTTCTCAA TCAGATGGTG GAGCAAGAGA GCCAAATCTT CCATGATATC
ATCGTGGAGG ACTTTATTGA CTCCTACCAT AACCTTACCC TCAAAACATT AATGGGGATG
AGATGGGTGG CCACTTTTTG TTCAAAAGCC AAGTATGTCA TGAAAACAGA CAGCGACATT
TTTGTAAACA TGGACAATCT TATTTATAAA TTACTGAAAC CCTCCACCAA GCCACGAAGA
AGGTATTTTA CTGGCTATGT CATTAATGGA GGACCGATTC GGGATGTCCG CAGTAAGTGG
TATATGCCCA GGGATTTGTA CCCAGACAGT AACTACCCAC CTTTCTGTTC GGGGACTGGC
TACATCTTTT CAGCCGATGT AGCTGAACTC ATTTACAAGA CCTCACTCCA CACAAGGCTG
CTTCACCTTG AAGACGTATA TGTGGGACTG TGTCTTCGAA AGCTGGGCAT ACATCCTTTC
CAGAACAGTG GCTTCAATCA CTGGAAAATG GCCTACAGTT TGTGTAGGTA TCGCCGAGTT
ATCACTGTGC ATCAGATCTC TCCAGAAGAA ATGCACAGAA TCTGGAATGA CATGTCAAGC
AAGAAACATC TCAGATGTTTA G

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.