×

SQSTM1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SQSTM1
Entrez Gene ID 8878
Full Name sequestosome 1
Synonyms A170, FTDALS3, OSIL, PDB3, ZIP3, p60, p62, p62B
General protein information
Preferred Names
sequestosome-1
Names
sequestosome-1
EBIAP
EBI3-associated protein p60
oxidative stress induced like
ubiquitin-binding protein p62
EBI3-associated protein of 60 kDa
phosphotyrosine independent ligand for the Lck SH2 domain p62
phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5q35

Summary This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]. lac of sum
Disorder MIM:

601530

Disorder Html: Paget disease of bone, 602080 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001142298 NP_001135770 sequestosome-1 isoform 2
NM_001142299 NP_001135771 sequestosome-1 isoform 2
NM_003900 NP_003891 sequestosome-1 isoform 1
XM_011534683 XP_011532985 sequestosome-1 isoform X1
XM_011534684 XP_011532986 sequestosome-1 isoform X2



Homo sapiens (human) SQSTM1 NP_003891.1
Pan troglodytes (chimpanzee) SQSTM1 XP_001153075.1
Macaca mulatta (Rhesus monkey) SQSTM1 XP_001102347.1
Bos taurus (cattle) SQSTM1 NP_788814.1
Mus musculus (house mouse) Sqstm1 NP_035148.1
Rattus norvegicus (Norway rat) Sqstm1 NP_787037.2
Gallus gallus (chicken) SQSTM1 XP_001233249.2
Danio rerio (zebrafish) sqstm1 NP_998338.1
Xenopus (Silurana) tropicalis (western clawed frog) sqstm1 NP_001007894.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SQSTM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SQSTM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26213
NM_001142298 Homo sapiens sequestosome 1 (SQSTM1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu26213
NM_001142299 Homo sapiens sequestosome 1 (SQSTM1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu26176
NM_003900 Homo sapiens sequestosome 1 (SQSTM1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu62276 XM_011534683 PREDICTED: Homo sapiens sequestosome 1 (SQSTM1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $139.30
$199.00
OHu62277 XM_011534684 PREDICTED: Homo sapiens sequestosome 1 (SQSTM1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26213
Clone ID Related Accession (Same CDS sequence) NM_001142298 , NM_001142299
Accession Version NM_001142298.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1071bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 03-JUL-2015
Organism Homo sapiens (human)
Product sequestosome-1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI549805.1, BC000951.2, BC017222.1 and BU625974.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation from an in-frame downstream start codon compared to variant 1. This results in an isoform (2) with a shorter N-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC001874.1, BC000951.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCCATGT CCTACGTGAA GGATGACATC TTCCGAATCT ACATTAAAGA GAAAAAAGAG 
TGCCGGCGGG ACCACCGCCC ACCGTGTGCT CAGGAGGCGC CCCGCAACAT GGTGCACCCC
AATGTGATCT GCGATGGCTG CAATGGGCCT GTGGTAGGAA CCCGCTACAA GTGCAGCGTC
TGCCCAGACT ACGACTTGTG TAGCGTCTGC GAGGGAAAGG GCTTGCACCG GGGGCACACC
AAGCTCGCAT TCCCCAGCCC CTTCGGGCAC CTGTCTGAGG GCTTCTCGCA CAGCCGCTGG
CTCCGGAAGG TGAAACACGG ACACTTCGGG TGGCCAGGAT GGGAAATGGG TCCACCAGGA
AACTGGAGCC CACGTCCTCC TCGTGCAGGG GAGGCCCGCC CTGGCCCCAC GGCAGAATCA
GCTTCTGGTC CATCGGAGGA TCCGAGTGTG AATTTCCTGA AGAACGTTGG GGAGAGTGTG
GCAGCTGCCC TTAGCCCTCT GGGCATTGAA GTTGATATCG ATGTGGAGCA CGGAGGGAAA
AGAAGCCGCC TGACCCCCGT CTCTCCAGAG AGTTCCAGCA CAGAGGAGAA GAGCAGCTCA
CAGCCAAGCA GCTGCTGCTC TGACCCCAGC AAGCCGGGTG GGAATGTTGA GGGCGCCACG
CAGTCTCTGG CGGAGCAGAT GAGGAAGATC GCCTTGGAGT CCGAGGGGCG CCCTGAGGAA
CAGATGGAGT CGGATAACTG TTCAGGAGGA GATGATGACT GGACCCATCT GTCTTCAAAA
GAAGTGGACC CGTCTACAGG TGAACTCCAG TCCCTACAGA TGCCAGAATC CGAAGGGCCA
AGCTCTCTGG ACCCCTCCCA GGAGGGACCC ACAGGGCTGA AGGAAGCTGC CTTGTACCCA
CATCTCCCGC CAGAGGCTGA CCCGCGGCTG ATTGAGTCCC TCTCCCAGAT GCTGTCCATG
GGCTTCTCTG ATGAAGGCGG CTGGCTCACC AGGCTCCTGC AGACCAAGAA CTATGACATC
GGAGCGGCTC TGGACACCAT CCAGTATTCA AAGCATCCCC CGCCGTTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001135770.1
CDS361..1431
Misc Feature(1)262..264(+)
Misc Feature(2)361..414(+)
Misc Feature(3)484..612(+)
Misc Feature(4)490..597(+)
Misc Feature(5)490..570(+)
Misc Feature(6)493..555(+)
Misc Feature(7)526..612(+)
Misc Feature(8)532..597(+)
Misc Feature(9)1285..1404(+)
Misc Feature(10)1318..1404(+)
Exon (1)1..204
Gene:SQSTM1
Gene Synonym:
Exon (2)205..313
Gene:SQSTM1
Gene Synonym:
Exon (3)314..409
Gene:SQSTM1
Gene Synonym:
Exon (4)410..639
Gene:SQSTM1
Gene Synonym:
Exon (5)640..781
Gene:SQSTM1
Gene Synonym:
Exon (6)782..862
Gene:SQSTM1
Gene Synonym:
Exon (7)863..1077
Gene:SQSTM1
Gene Synonym:
Exon (8)1078..1273
Gene:SQSTM1
Gene Synonym:
Exon (9)1274..2916
Gene:SQSTM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001142298
Organism Homo sapiens (human)
Definition Homo sapiens sequestosome 1 (SQSTM1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001142298

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCCATGT CCTACGTGAA GGATGACATC TTCCGAATCT ACATTAAAGA GAAAAAAGAG 
TGCCGGCGGG ACCACCGCCC ACCGTGTGCT CAGGAGGCGC CCCGCAACAT GGTGCACCCC
AATGTGATCT GCGATGGCTG CAATGGGCCT GTGGTAGGAA CCCGCTACAA GTGCAGCGTC
TGCCCAGACT ACGACTTGTG TAGCGTCTGC GAGGGAAAGG GCTTGCACCG GGGGCACACC
AAGCTCGCAT TCCCCAGCCC CTTCGGGCAC CTGTCTGAGG GCTTCTCGCA CAGCCGCTGG
CTCCGGAAGG TGAAACACGG ACACTTCGGG TGGCCAGGAT GGGAAATGGG TCCACCAGGA
AACTGGAGCC CACGTCCTCC TCGTGCAGGG GAGGCCCGCC CTGGCCCCAC GGCAGAATCA
GCTTCTGGTC CATCGGAGGA TCCGAGTGTG AATTTCCTGA AGAACGTTGG GGAGAGTGTG
GCAGCTGCCC TTAGCCCTCT GGGCATTGAA GTTGATATCG ATGTGGAGCA CGGAGGGAAA
AGAAGCCGCC TGACCCCCGT CTCTCCAGAG AGTTCCAGCA CAGAGGAGAA GAGCAGCTCA
CAGCCAAGCA GCTGCTGCTC TGACCCCAGC AAGCCGGGTG GGAATGTTGA GGGCGCCACG
CAGTCTCTGG CGGAGCAGAT GAGGAAGATC GCCTTGGAGT CCGAGGGGCG CCCTGAGGAA
CAGATGGAGT CGGATAACTG TTCAGGAGGA GATGATGACT GGACCCATCT GTCTTCAAAA
GAAGTGGACC CGTCTACAGG TGAACTCCAG TCCCTACAGA TGCCAGAATC CGAAGGGCCA
AGCTCTCTGG ACCCCTCCCA GGAGGGACCC ACAGGGCTGA AGGAAGCTGC CTTGTACCCA
CATCTCCCGC CAGAGGCTGA CCCGCGGCTG ATTGAGTCCC TCTCCCAGAT GCTGTCCATG
GGCTTCTCTG ATGAAGGCGG CTGGCTCACC AGGCTCCTGC AGACCAAGAA CTATGACATC
GGAGCGGCTC TGGACACCAT CCAGTATTCA AAGCATCCCC CGCCGTTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26213
Clone ID Related Accession (Same CDS sequence) NM_001142298 , NM_001142299
Accession Version NM_001142299.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1071bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 03-JUL-2015
Organism Homo sapiens (human)
Product sequestosome-1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA541885.1, BC000951.2, BC017222.1 and BU625974.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation from an in-frame downstream start codon compared to variant 1. This results in an isoform (2) with a shorter N-terminus compared to isoform 1. Variants 2 and 3 differ in the 5' UTR, but encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC001874.1, BC000951.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCCATGT CCTACGTGAA GGATGACATC TTCCGAATCT ACATTAAAGA GAAAAAAGAG 
TGCCGGCGGG ACCACCGCCC ACCGTGTGCT CAGGAGGCGC CCCGCAACAT GGTGCACCCC
AATGTGATCT GCGATGGCTG CAATGGGCCT GTGGTAGGAA CCCGCTACAA GTGCAGCGTC
TGCCCAGACT ACGACTTGTG TAGCGTCTGC GAGGGAAAGG GCTTGCACCG GGGGCACACC
AAGCTCGCAT TCCCCAGCCC CTTCGGGCAC CTGTCTGAGG GCTTCTCGCA CAGCCGCTGG
CTCCGGAAGG TGAAACACGG ACACTTCGGG TGGCCAGGAT GGGAAATGGG TCCACCAGGA
AACTGGAGCC CACGTCCTCC TCGTGCAGGG GAGGCCCGCC CTGGCCCCAC GGCAGAATCA
GCTTCTGGTC CATCGGAGGA TCCGAGTGTG AATTTCCTGA AGAACGTTGG GGAGAGTGTG
GCAGCTGCCC TTAGCCCTCT GGGCATTGAA GTTGATATCG ATGTGGAGCA CGGAGGGAAA
AGAAGCCGCC TGACCCCCGT CTCTCCAGAG AGTTCCAGCA CAGAGGAGAA GAGCAGCTCA
CAGCCAAGCA GCTGCTGCTC TGACCCCAGC AAGCCGGGTG GGAATGTTGA GGGCGCCACG
CAGTCTCTGG CGGAGCAGAT GAGGAAGATC GCCTTGGAGT CCGAGGGGCG CCCTGAGGAA
CAGATGGAGT CGGATAACTG TTCAGGAGGA GATGATGACT GGACCCATCT GTCTTCAAAA
GAAGTGGACC CGTCTACAGG TGAACTCCAG TCCCTACAGA TGCCAGAATC CGAAGGGCCA
AGCTCTCTGG ACCCCTCCCA GGAGGGACCC ACAGGGCTGA AGGAAGCTGC CTTGTACCCA
CATCTCCCGC CAGAGGCTGA CCCGCGGCTG ATTGAGTCCC TCTCCCAGAT GCTGTCCATG
GGCTTCTCTG ATGAAGGCGG CTGGCTCACC AGGCTCCTGC AGACCAAGAA CTATGACATC
GGAGCGGCTC TGGACACCAT CCAGTATTCA AAGCATCCCC CGCCGTTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001135771.1
CDS278..1348
Misc Feature(1)179..181(+)
Misc Feature(2)278..331(+)
Misc Feature(3)401..529(+)
Misc Feature(4)407..514(+)
Misc Feature(5)407..487(+)
Misc Feature(6)410..472(+)
Misc Feature(7)443..529(+)
Misc Feature(8)449..514(+)
Misc Feature(9)1202..1321(+)
Misc Feature(10)1235..1321(+)
Exon (1)1..121
Gene:SQSTM1
Gene Synonym:
Exon (2)122..230
Gene:SQSTM1
Gene Synonym:
Exon (3)231..326
Gene:SQSTM1
Gene Synonym:
Exon (4)327..556
Gene:SQSTM1
Gene Synonym:
Exon (5)557..698
Gene:SQSTM1
Gene Synonym:
Exon (6)699..779
Gene:SQSTM1
Gene Synonym:
Exon (7)780..994
Gene:SQSTM1
Gene Synonym:
Exon (8)995..1190
Gene:SQSTM1
Gene Synonym:
Exon (9)1191..2833
Gene:SQSTM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001142299
Organism Homo sapiens (human)
Definition Homo sapiens sequestosome 1 (SQSTM1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001142299

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCCATGT CCTACGTGAA GGATGACATC TTCCGAATCT ACATTAAAGA GAAAAAAGAG 
TGCCGGCGGG ACCACCGCCC ACCGTGTGCT CAGGAGGCGC CCCGCAACAT GGTGCACCCC
AATGTGATCT GCGATGGCTG CAATGGGCCT GTGGTAGGAA CCCGCTACAA GTGCAGCGTC
TGCCCAGACT ACGACTTGTG TAGCGTCTGC GAGGGAAAGG GCTTGCACCG GGGGCACACC
AAGCTCGCAT TCCCCAGCCC CTTCGGGCAC CTGTCTGAGG GCTTCTCGCA CAGCCGCTGG
CTCCGGAAGG TGAAACACGG ACACTTCGGG TGGCCAGGAT GGGAAATGGG TCCACCAGGA
AACTGGAGCC CACGTCCTCC TCGTGCAGGG GAGGCCCGCC CTGGCCCCAC GGCAGAATCA
GCTTCTGGTC CATCGGAGGA TCCGAGTGTG AATTTCCTGA AGAACGTTGG GGAGAGTGTG
GCAGCTGCCC TTAGCCCTCT GGGCATTGAA GTTGATATCG ATGTGGAGCA CGGAGGGAAA
AGAAGCCGCC TGACCCCCGT CTCTCCAGAG AGTTCCAGCA CAGAGGAGAA GAGCAGCTCA
CAGCCAAGCA GCTGCTGCTC TGACCCCAGC AAGCCGGGTG GGAATGTTGA GGGCGCCACG
CAGTCTCTGG CGGAGCAGAT GAGGAAGATC GCCTTGGAGT CCGAGGGGCG CCCTGAGGAA
CAGATGGAGT CGGATAACTG TTCAGGAGGA GATGATGACT GGACCCATCT GTCTTCAAAA
GAAGTGGACC CGTCTACAGG TGAACTCCAG TCCCTACAGA TGCCAGAATC CGAAGGGCCA
AGCTCTCTGG ACCCCTCCCA GGAGGGACCC ACAGGGCTGA AGGAAGCTGC CTTGTACCCA
CATCTCCCGC CAGAGGCTGA CCCGCGGCTG ATTGAGTCCC TCTCCCAGAT GCTGTCCATG
GGCTTCTCTG ATGAAGGCGG CTGGCTCACC AGGCTCCTGC AGACCAAGAA CTATGACATC
GGAGCGGCTC TGGACACCAT CCAGTATTCA AAGCATCCCC CGCCGTTGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26176
Clone ID Related Accession (Same CDS sequence) NM_003900
Accession Version NM_003900.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1323bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 03-JUL-2015
Organism Homo sapiens (human)
Product sequestosome-1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC398116.1, BC000951.2, BC017222.1 and AI333228.1. On May 16, 2008 this sequence version replaced gi:46251280. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017222.1, U46751.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGTCGC TCACCGTGAA GGCCTACCTT CTGGGCAAGG AGGACGCGGC GCGCGAGATT 
CGCCGCTTCA GCTTCTGCTG CAGCCCCGAG CCTGAGGCGG AAGCCGAGGC TGCGGCGGGT
CCGGGACCCT GCGAGCGGCT GCTGAGCCGG GTGGCCGCCC TGTTCCCCGC GCTGCGGCCT
GGCGGCTTCC AGGCGCACTA CCGCGATGAG GACGGGGACT TGGTTGCCTT TTCCAGTGAC
GAGGAATTGA CAATGGCCAT GTCCTACGTG AAGGATGACA TCTTCCGAAT CTACATTAAA
GAGAAAAAAG AGTGCCGGCG GGACCACCGC CCACCGTGTG CTCAGGAGGC GCCCCGCAAC
ATGGTGCACC CCAATGTGAT CTGCGATGGC TGCAATGGGC CTGTGGTAGG AACCCGCTAC
AAGTGCAGCG TCTGCCCAGA CTACGACTTG TGTAGCGTCT GCGAGGGAAA GGGCTTGCAC
CGGGGGCACA CCAAGCTCGC ATTCCCCAGC CCCTTCGGGC ACCTGTCTGA GGGCTTCTCG
CACAGCCGCT GGCTCCGGAA GGTGAAACAC GGACACTTCG GGTGGCCAGG ATGGGAAATG
GGTCCACCAG GAAACTGGAG CCCACGTCCT CCTCGTGCAG GGGAGGCCCG CCCTGGCCCC
ACGGCAGAAT CAGCTTCTGG TCCATCGGAG GATCCGAGTG TGAATTTCCT GAAGAACGTT
GGGGAGAGTG TGGCAGCTGC CCTTAGCCCT CTGGGCATTG AAGTTGATAT CGATGTGGAG
CACGGAGGGA AAAGAAGCCG CCTGACCCCC GTCTCTCCAG AGAGTTCCAG CACAGAGGAG
AAGAGCAGCT CACAGCCAAG CAGCTGCTGC TCTGACCCCA GCAAGCCGGG TGGGAATGTT
GAGGGCGCCA CGCAGTCTCT GGCGGAGCAG ATGAGGAAGA TCGCCTTGGA GTCCGAGGGG
CGCCCTGAGG AACAGATGGA GTCGGATAAC TGTTCAGGAG GAGATGATGA CTGGACCCAT
CTGTCTTCAA AAGAAGTGGA CCCGTCTACA GGTGAACTCC AGTCCCTACA GATGCCAGAA
TCCGAAGGGC CAAGCTCTCT GGACCCCTCC CAGGAGGGAC CCACAGGGCT GAAGGAAGCT
GCCTTGTACC CACATCTCCC GCCAGAGGCT GACCCGCGGC TGATTGAGTC CCTCTCCCAG
ATGCTGTCCA TGGGCTTCTC TGATGAAGGC GGCTGGCTCA CCAGGCTCCT GCAGACCAAG
AACTATGACA TCGGAGCGGC TCTGGACACC ATCCAGTATT CAAAGCATCC CCCGCCGTTG
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003891.1
CDS96..1418
Misc Feature(1)99..245(+)
Misc Feature(2)99..101(+)
Misc Feature(3)105..401(+)
Misc Feature(4)165..167(+)
Misc Feature(5)177..179(+)
Misc Feature(6)222..416(+)
Misc Feature(7)243..335(+)
Misc Feature(8)360..362(+)
Misc Feature(9)459..767(+)
Misc Feature(10)471..599(+)
Misc Feature(11)477..584(+)
Misc Feature(12)477..557(+)
Misc Feature(13)480..542(+)
Misc Feature(14)513..599(+)
Misc Feature(15)519..584(+)
Misc Feature(16)537..539(+)
Misc Feature(17)603..755(+)
Misc Feature(18)603..605(+)
Misc Feature(19)621..623(+)
Misc Feature(20)633..635(+)
Misc Feature(21)639..641(+)
Misc Feature(22)714..716(+)
Misc Feature(23)777..794(+)
Misc Feature(24)792..794(+)
Misc Feature(25)840..842(+)
Misc Feature(26)891..893(+)
Misc Feature(27)891..893(+)
Misc Feature(28)900..1415(+)
Misc Feature(29)900..902(+)
Misc Feature(30)900..902(+)
Misc Feature(31)900..902(+)
Misc Feature(32)909..911(+)
Misc Feature(33)909..911(+)
Misc Feature(34)909..911(+)
Misc Feature(35)918..920(+)
Misc Feature(36)921..923(+)
Misc Feature(37)924..926(+)
Misc Feature(38)927..929(+)
Misc Feature(39)939..941(+)
Misc Feature(40)942..944(+)
Misc Feature(41)945..947(+)
Misc Feature(42)1011..1013(+)
Misc Feature(43)1047..1049(+)
Misc Feature(44)1056..1121(+)
Misc Feature(45)1077..1079(+)
Misc Feature(46)1077..1079(+)
Misc Feature(47)1089..1091(+)
Misc Feature(48)1089..1091(+)
Misc Feature(49)1101..1118(+)
Misc Feature(50)1110..1112(+)
Misc Feature(51)1119..1121(+)
Misc Feature(52)1122..1124(+)
Misc Feature(53)1158..1160(+)
Misc Feature(54)1176..1178(+)
Misc Feature(55)1188..1190(+)
Misc Feature(56)1191..1193(+)
Misc Feature(57)1191..1193(+)
Misc Feature(58)1203..1205(+)
Misc Feature(59)1218..1220(+)
Misc Feature(60)1272..1391(+)
Misc Feature(61)1305..1391(+)
Exon (1)1..300
Gene:SQSTM1
Gene Synonym:
Exon (2)301..396
Gene:SQSTM1
Gene Synonym:
Exon (3)397..626
Gene:SQSTM1
Gene Synonym:
Exon (4)627..768
Gene:SQSTM1
Gene Synonym:
Exon (5)769..849
Gene:SQSTM1
Gene Synonym:
Exon (6)850..1064
Gene:SQSTM1
Gene Synonym:
Exon (7)1065..1260
Gene:SQSTM1
Gene Synonym:
Exon (8)1261..2903
Gene:SQSTM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003900
Organism Homo sapiens (human)
Definition Homo sapiens sequestosome 1 (SQSTM1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003900

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGTCGC TCACCGTGAA GGCCTACCTT CTGGGCAAGG AGGACGCGGC GCGCGAGATT 
CGCCGCTTCA GCTTCTGCTG CAGCCCCGAG CCTGAGGCGG AAGCCGAGGC TGCGGCGGGT
CCGGGACCCT GCGAGCGGCT GCTGAGCCGG GTGGCCGCCC TGTTCCCCGC GCTGCGGCCT
GGCGGCTTCC AGGCGCACTA CCGCGATGAG GACGGGGACT TGGTTGCCTT TTCCAGTGAC
GAGGAATTGA CAATGGCCAT GTCCTACGTG AAGGATGACA TCTTCCGAAT CTACATTAAA
GAGAAAAAAG AGTGCCGGCG GGACCACCGC CCACCGTGTG CTCAGGAGGC GCCCCGCAAC
ATGGTGCACC CCAATGTGAT CTGCGATGGC TGCAATGGGC CTGTGGTAGG AACCCGCTAC
AAGTGCAGCG TCTGCCCAGA CTACGACTTG TGTAGCGTCT GCGAGGGAAA GGGCTTGCAC
CGGGGGCACA CCAAGCTCGC ATTCCCCAGC CCCTTCGGGC ACCTGTCTGA GGGCTTCTCG
CACAGCCGCT GGCTCCGGAA GGTGAAACAC GGACACTTCG GGTGGCCAGG ATGGGAAATG
GGTCCACCAG GAAACTGGAG CCCACGTCCT CCTCGTGCAG GGGAGGCCCG CCCTGGCCCC
ACGGCAGAAT CAGCTTCTGG TCCATCGGAG GATCCGAGTG TGAATTTCCT GAAGAACGTT
GGGGAGAGTG TGGCAGCTGC CCTTAGCCCT CTGGGCATTG AAGTTGATAT CGATGTGGAG
CACGGAGGGA AAAGAAGCCG CCTGACCCCC GTCTCTCCAG AGAGTTCCAG CACAGAGGAG
AAGAGCAGCT CACAGCCAAG CAGCTGCTGC TCTGACCCCA GCAAGCCGGG TGGGAATGTT
GAGGGCGCCA CGCAGTCTCT GGCGGAGCAG ATGAGGAAGA TCGCCTTGGA GTCCGAGGGG
CGCCCTGAGG AACAGATGGA GTCGGATAAC TGTTCAGGAG GAGATGATGA CTGGACCCAT
CTGTCTTCAA AAGAAGTGGA CCCGTCTACA GGTGAACTCC AGTCCCTACA GATGCCAGAA
TCCGAAGGGC CAAGCTCTCT GGACCCCTCC CAGGAGGGAC CCACAGGGCT GAAGGAAGCT
GCCTTGTACC CACATCTCCC GCCAGAGGCT GACCCGCGGC TGATTGAGTC CCTCTCCCAG
ATGCTGTCCA TGGGCTTCTC TGATGAAGGC GGCTGGCTCA CCAGGCTCCT GCAGACCAAG
AACTATGACA TCGGAGCGGC TCTGGACACC ATCCAGTATT CAAAGCATCC CCCGCCGTTG
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62276
Clone ID Related Accession (Same CDS sequence) XM_011534683
Accession Version XM_011534683.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 732bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sequestosome-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_023133.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCCCGGG GGACCGGGGC CGGGGCGCAG GGGTCGGAAG GCGGCGGCGG CGGCGGCAGG 
GGCCCCGGCC CCGGGTCGGG GAGGGGCGGG GGGCCCGGGG CCGGGCGGGG ACCGGGCCAG
GGAGCGCGCC GGCCGCCCCT CAGGGCGCAA GCTTTGTGCC CTGTACTCAG GGAAGAGGAA
CAGGCTCAGA AGGGCAGAGG CAGGTATCAG GCTCACTGCA GATATCAGGG GCGCGGGACA
CTGGCGGCCT CGCCTCCGCG GCAGGGCCGG GCCGGGCCGG GCTGGGCTGG GCTGGGCGGC
GAGAGCCGCG GCCCGGCCTG GATCTGGGGC CTGGATCTGG GGCCGCCGCG GAGTCGACGG
CGCAGGGCGG GGCGGCCCGG ATTTAAAGGG GCCGCAGCAC CGCCGTCGCC GGCGCCGCGA
GGGGGTGGGG TGGGGGCCGG CGGCCGGGAT CCCGATCGGC TCCCGCAGCC CCGCGTGGGC
TCGTGCGAGT CGGCCTCAGT GTCTGCGAGA TTAATCTCTC ATGGCCGCTG CACAAGAACC
TGGCTTTTAG CTGAACTAAG GAGAAAGTCC TACAACAGTT TGGCGTGCAA CATGGGGCTT
GAGAAAGGGT TTTCAGCAGC TTCCGGAGGC CGCCTGGACT GCAGAAGGCG CCCTGGGCGT
GTGTACTTCC ACCTCCGCAG GCGCTGGCAT CTCCAGGCTG CTTCAGGCCG CTTTGGACCT
CACCATCGGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532985.1
CDS16..747
Translation

Target ORF information:

RefSeq Version XM_011534683
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sequestosome 1 (SQSTM1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534683

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCCCGGG GGACCGGGGC CGGGGCGCAG GGGTCGGAAG GCGGCGGCGG CGGCGGCAGG 
GGCCCCGGCC CCGGGTCGGG GAGGGGCGGG GGGCCCGGGG CCGGGCGGGG ACCGGGCCAG
GGAGCGCGCC GGCCGCCCCT CAGGGCGCAA GCTTTGTGCC CTGTACTCAG GGAAGAGGAA
CAGGCTCAGA AGGGCAGAGG CAGGTATCAG GCTCACTGCA GATATCAGGG GCGCGGGACA
CTGGCGGCCT CGCCTCCGCG GCAGGGCCGG GCCGGGCCGG GCTGGGCTGG GCTGGGCGGC
GAGAGCCGCG GCCCGGCCTG GATCTGGGGC CTGGATCTGG GGCCGCCGCG GAGTCGACGG
CGCAGGGCGG GGCGGCCCGG ATTTAAAGGG GCCGCAGCAC CGCCGTCGCC GGCGCCGCGA
GGGGGTGGGG TGGGGGCCGG CGGCCGGGAT CCCGATCGGC TCCCGCAGCC CCGCGTGGGC
TCGTGCGAGT CGGCCTCAGT GTCTGCGAGA TTAATCTCTC ATGGCCGCTG CACAAGAACC
TGGCTTTTAG CTGAACTAAG GAGAAAGTCC TACAACAGTT TGGCGTGCAA CATGGGGCTT
GAGAAAGGGT TTTCAGCAGC TTCCGGAGGC CGCCTGGACT GCAGAAGGCG CCCTGGGCGT
GTGTACTTCC ACCTCCGCAG GCGCTGGCAT CTCCAGGCTG CTTCAGGCCG CTTTGGACCT
CACCATCGGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62277
Clone ID Related Accession (Same CDS sequence) XM_011534684
Accession Version XM_011534684.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 663bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product sequestosome-1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_023133.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCCCGGG GGACCGGGGC CGGGGCGCAG GGGTCGGAAG GCGGCGGCGG CGGCGGCAGG 
GGCCCCGGCC CCGGGTCGGG GAGGGGCGGG GGGCCCGGGG CCGGGCGGGG ACCGGGCCAG
GGAGCGCGCC GGCCGCCCCT CAGGGCGCAA GCTTTGTGCC CTGTACTCAG GGAAGAGGAA
CAGGCTCAGA AGGGCAGAGG CAGGTATCAG GCTCACTGCA GATATCAGGG GCGCGGGACA
CTGGCGGCCT CGCCTCCGCG GCAGGGCCGG GCCGGGCCGG GCTGGGCTGG GCTGGGCGGC
GAGAGCCGCG GCCCGGCCTG GATCTGGGGC CTGGATCTGG GGCCGCCGCG GAGTCGACGG
CGCAGGGCGG GGCGGCCCGG ATTTAAAGGG GCCGCAGCAC CGCCGTCGCC GGCGCCGCGA
GGGGGTGGGG TGGGGGCCGG CGGCCGGGAT CCCGATCGGC TCCCGCAGCC CCGCGTGGGC
TCGTGCGAGT CGGCCTCAGT GTCTGCGAGA TTAATCTCTC ATGGCCGCTG CACAAGAACC
TGGCTTTTAG CTGAACTAAG GAGAAAGTCC TACAACAGTT TGGCGTGCAA CATGGGGCTT
GAGAAAGGGC CCCAGATGCT GAATCTAAGA GGGCAGAGGA AAAGCTTTTC CTCCCTGACA
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532986.1
CDS16..678
Translation

Target ORF information:

RefSeq Version XM_011534684
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sequestosome 1 (SQSTM1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534684

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCCCGGG GGACCGGGGC CGGGGCGCAG GGGTCGGAAG GCGGCGGCGG CGGCGGCAGG 
GGCCCCGGCC CCGGGTCGGG GAGGGGCGGG GGGCCCGGGG CCGGGCGGGG ACCGGGCCAG
GGAGCGCGCC GGCCGCCCCT CAGGGCGCAA GCTTTGTGCC CTGTACTCAG GGAAGAGGAA
CAGGCTCAGA AGGGCAGAGG CAGGTATCAG GCTCACTGCA GATATCAGGG GCGCGGGACA
CTGGCGGCCT CGCCTCCGCG GCAGGGCCGG GCCGGGCCGG GCTGGGCTGG GCTGGGCGGC
GAGAGCCGCG GCCCGGCCTG GATCTGGGGC CTGGATCTGG GGCCGCCGCG GAGTCGACGG
CGCAGGGCGG GGCGGCCCGG ATTTAAAGGG GCCGCAGCAC CGCCGTCGCC GGCGCCGCGA
GGGGGTGGGG TGGGGGCCGG CGGCCGGGAT CCCGATCGGC TCCCGCAGCC CCGCGTGGGC
TCGTGCGAGT CGGCCTCAGT GTCTGCGAGA TTAATCTCTC ATGGCCGCTG CACAAGAACC
TGGCTTTTAG CTGAACTAAG GAGAAAGTCC TACAACAGTT TGGCGTGCAA CATGGGGCTT
GAGAAAGGGC CCCAGATGCT GAATCTAAGA GGGCAGAGGA AAAGCTTTTC CTCCCTGACA
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Levels of metacaspase1 and chaperones related to protein quality control in alcoholic and nonalcoholic steatohepatitis
Exp. Mol. Pathol. 98 (1), 65-72 (2015)
Mendoza AS, Dorce J, Peng Y, French BA, Tillman B, Li J and French SW.


book

Kaposi's sarcoma-associated herpesvirus induces Nrf2 activation in latently infected endothelial cells through SQSTM1 phosphorylation and interaction with polyubiquitinated Keap1
J. Virol. 89 (4), 2268-2286 (2015)
Gjyshi O, Flaherty S, Veettil MV, Johnson KE, Chandran B and Bottero V.


book

Autophagy may occur at an early stage of cholangiocarcinogenesis via biliary intraepithelial neoplasia
Hum. Pathol. 46 (2), 202-209 (2015)
Sasaki M, Nitta T, Sato Y and Nakanuma Y.


book

Interferon-stimulated gene 15 (ISG15) and ISG15-linked proteins can associate with members of the selective autophagic process, histone deacetylase 6 (HDAC6) and SQSTM1/p62
J. Biol. Chem. 290 (3), 1485-1495 (2015)
Nakashima H, Nguyen T, Goins WF and Chiocca EA.


book

RIPK3 regulates p62-LC3 complex formation via the caspase-8-dependent cleavage of p62
Biochem. Biophys. Res. Commun. 456 (1), 298-304 (2015)
Matsuzawa Y, Oshima S, Nibe Y, Kobayashi M, Maeyashiki C, Nemoto Y, Nagaishi T, Okamoto R, Tsuchiya K, Nakamura T and Watanabe M.


book

A p56(lck) ligand serves as a coactivator of an orphan nuclear hormone receptor
J. Biol. Chem. 271 (44), 27197-27200 (1996)
Marcus SL, Winrow CJ, Capone JP and Rachubinski RA.


book

p62, a phosphotyrosine-independent ligand of the SH2 domain of p56lck, belongs to a new class of ubiquitin-binding proteins
J. Biol. Chem. 271 (34), 20235-20237 (1996)
Vadlamudi RK, Joung I, Strominger JL and Shin J.


book

Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain
Proc. Natl. Acad. Sci. U.S.A. 93 (12), 5991-5995 (1996)
Joung I, Strominger JL and Shin J.


book

A novel interleukin-12 p40-related protein induced by latent Epstein-Barr virus infection in B lymphocytes
J. Virol. 70 (2), 1143-1153 (1996)
Devergne O, Hummel M, Koeppen H, Le Beau MM, Nathanson EC, Kieff E and Birkenbach M.


book

Phosphotyrosine-independent binding of a 62-kDa protein to the src homology 2 (SH2) domain of p56lck and its regulation by phosphorylation of Ser-59 in the lck unique N-terminal region
Proc. Natl. Acad. Sci. U.S.A. 92 (26), 12338-12342 (1995)
Park I, Chung J, Walsh CT, Yun Y, Strominger JL and Shin J.


 
*
*
*
*