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EIF2B4 eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa [Homo sapiens (human)]

Gene Symbol EIF2B4
Entrez Gene ID 8890
Full Name eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa
Synonyms EIF-2B, EIF2B, EIF2Bdelta
General protein information
Preferred Names
translation initiation factor eIF-2B subunit delta
Names
translation initiation factor eIF-2B subunit delta
eIF-2B GDP-GTP exchange factor subunit delta
translation initiation factor eIF-2b delta subunit
eukaryotic translation initiation factor 2B subunit 4 delta
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p23.3

Summary Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

606687

Disorder Html: Leukoencephaly with vanishing white matter, 603896 (3);





The following EIF2B4 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2B4 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu35652 XM_005264632 PREDICTED: Homo sapiens eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $439
OHu35653 XM_006712132 PREDICTED: Homo sapiens eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu62291 XM_011533147 PREDICTED: Homo sapiens eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199
OHu22903 NM_015636 Homo sapiens eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379
OHu22714 NM_001034116 Homo sapiens eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$379
OHu27281 NM_172195 Homo sapiens eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $459

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu35652
Accession Version XM_005264632.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1527bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product translation initiation factor eIF-2B subunit delta isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAAGGCGG AGCTTCCCCC TGGGCCTGGG GCAGTGGGGA GGGAAATGAC CAAAGAAGAA 
AAGCTGCAGC TTCGGAAGGA AAAGAAACAG CAGAAGAAGA AACGGAAGGA AGAAAAGGGG
GCAGAACCAG AGACTGGCTC TGCTGTATCT GCAGCCCAAT GTCAAGTAGG CCCAACCAGA
GAACTGCCAG AATCGGGCAT TCAGTTGGGC ACTCCTCGGG AGAAAGTTCC AGCTGGTCGG
AGTAAGGCCG AACTTCGGGC TGAGCGTCGA GCCAAGCAGG AGGCCGAGCG GGCCCTGAAA
CAGGCAAGAA AAGGGGAACA AGGAGGACCA CCTCCTAAGG CCAGCCCCAG CACAGCTGGA
GAAACCCCCT CAGGAGTGAA GCGTCTCCCT GAGTACCCTC AGGTTGATGA CCTACTTCTG
AGAAGGCTTG TTAAAAAACC AGAGCGTCAA CAGGTTCCTA CACGAAAGGA TTATGGATCC
AAAGTCAGTC TCTTCTCTCA CCTACCCCAG TACAGCAGAC AAAACTCTCT GACCCAGTTT
ATGAGCATCC CATCCTCTGT GATCCACCCA GCCATGGTGC GACTCGGCCT GCAGTACTCC
CAGGGCCTGG TCAGTGGCTC CAATGCCCGG TGTATTGCCC TGCTTCGTGC CTTGCAGCAG
GTGATTCAGG ATTACACAAC ACCGCCTAAT GAAGAACTCT CCAGGGATCT AGTGAATAAA
CTAAAACCCT ACATGAGCTT CCTGACTCAG TGCCGTCCCC TGTCAGCGAG CATGCACAAC
GCCATCAAGT TCCTTAACAA GGAAATCACC AGTGTGGGCA GTTCCAAGCG GGAAGAGGAG
GCCAAGTCAG AACTTCGAGC AGCCATTGAT CGGTATGTGC AAGAGAAGAT TGTGCTAGCA
GCTCAGGCAA TTTCACGCTT TGCTTACCAG AAGATCAGTA ATGGAGATGT GATCCTGGTA
TATGGATGCT CATCTCTGGT ATCACGAATT CTTCAGGAGG CTTGGACAGA GGGCCGGCGG
TTTCGGGTGG TAGTGGTGGA CAGCCGGCCA TGGCTGGAAG GAAGGCACAC ACTACGTTCT
CTAGTCCATG CTGGTGTCCC AGCCTCCTAC CTGCTGATTC CTGCAGCCTC CTATGTGCTC
CCAGAGGTTT CCAAGGTGCT ATTGGGAGCT CATGCACTCT TGGCCAACGG GTCTGTGATG
TCACGGGTAG GGACAGCACA GTTAGCCCTG GTGGCTCGAG CCCATAATGT ACCAGTGCTG
GTTTGCTGTG AAACATACAA GTTCTGTGAG CGTGTGCAGA CTGATGCCTT TGTCTCTAAT
GAGCTAGATG ACCCTGATGA TCTGCAATGT AAGCGGGGAG AACATGTTGC GCTGGCTAAC
TGGCAGAACC ACGCATCCCT ACGGTTGTTG AATCTAGTCT ATGATGTGAC TCCCCCAGAG
CTTGTGGATC TGGTGATCAC GGAGCTGGGG ATGATCCCTT GCAGTTCTGT ACCTGTTGTT
CTACGAGTCA AGAGCAGTGA CCAGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005264689.1
CDS210..1736
Misc Feature(1)819..1715(+)
Misc Feature(2)819..1691(+)
Translation
Position Chain Variation Link
1 1 c, t dbSNP:575951063
6 6 c, g, t dbSNP:764607200
10 10 c, t dbSNP:760688449
11 11 c, t dbSNP:368043444
13 13 c, t dbSNP:113310794
14 14 g, t dbSNP:542570644
16 16 a, g dbSNP:759657701
18 18 a, g dbSNP:774674616
29 29 a, g dbSNP:769436465
30 30 c, t dbSNP:747983242
32 32 a, g dbSNP:781222070
50 50 a, g dbSNP:745438083
52 52 a, g dbSNP:371347853
55 55 c, g dbSNP:747012688
57 57 c, t dbSNP:575101634
60 60 c, g dbSNP:553359107
68 68 a, g dbSNP:367784417
82 82 c, g dbSNP:748747375
83 83 a, g dbSNP:577115995
87 87 c, t dbSNP:558671461
110 110 c, t dbSNP:374204525
113 113 c, t dbSNP:537135294
120 120 a, g dbSNP:568745662
151 151 c, t dbSNP:375551474
162 162 c, t dbSNP:550339577
168 168 c, t dbSNP:141681782
190 190 a, g dbSNP:568357849
200 200 a, g dbSNP:778090500
201 201 a, g dbSNP:756513459
206 206 c, t dbSNP:753104792
215 215 c, g dbSNP:190259653
225 225 c, t dbSNP:754958652
227 227 c, t dbSNP:751668695
229 229 -, c dbSNP:764709491
229 229 c, t dbSNP:766637381
231 231 c, g dbSNP:769672829
233 233 g, t dbSNP:559997020
238 238 g, t dbSNP:137963149
240 240 c, g dbSNP:747925994
241 241 a, c dbSNP:752068283
244 244 c, t dbSNP:766541364
245 245 a, g dbSNP:758587510
248 248 -, g dbSNP:761106515
248 248 a, g dbSNP:750630633
252 252 c, g dbSNP:780113294
257 257 a, g dbSNP:760328185
264 264 a, g dbSNP:775357878
269 269 a, g dbSNP:767281618
272 272 a, g dbSNP:201350814
273 273 c, g dbSNP:759503915
276 276 c, g dbSNP:144798741
289 289 a, t dbSNP:774312870
293 293 a, g dbSNP:770388291
298 298 a, g dbSNP:748820541
303 303 a, c dbSNP:368905502
305 305 -, gaagaa dbSNP:775790323
308 308 -, gaa dbSNP:772427644
308 308 a, g dbSNP:769443508
320 320 a, g dbSNP:747824895
327 327 a, g dbSNP:756565356
328 328 a, g dbSNP:781612114
329 329 g, t dbSNP:768953969
330 330 a, g dbSNP:747515908
344 344 a, t dbSNP:780733138
346 346 g, t dbSNP:139521128
349 349 c, t dbSNP:750540614
352 352 c, t dbSNP:779208304
353 353 g, t dbSNP:377209171
357 357 g, t dbSNP:754066143
360 360 a, g dbSNP:200290985
367 367 a, g dbSNP:759281615
368 368 a, g dbSNP:751451207
369 369 c, t dbSNP:766318671
380 380 c, t dbSNP:1141530
383 383 a, c dbSNP:750413412
385 385 c, t dbSNP:764777030
386 386 c, t dbSNP:547931123
402 402 a, t dbSNP:776294002
403 403 c, t dbSNP:763729559
404 404 a, g dbSNP:368615356
413 413 a, g, t dbSNP:371589780
419 419 c, t dbSNP:775992226
420 420 a, t dbSNP:530363694
424 424 -, ctc dbSNP:769733183
426 426 c, g, t dbSNP:774760656
427 427 a, g dbSNP:146262118
434 434 a, g dbSNP:770994421
442 442 c, t dbSNP:34155621
443 443 c, t dbSNP:373805172
446 446 a, t dbSNP:777985531
447 447 c, t dbSNP:756288383
455 455 a, g dbSNP:201950471
458 458 c, g dbSNP:779806044
459 459 a, g dbSNP:758233858
466 466 c, g, t dbSNP:368061442
467 467 a, g dbSNP:369882701
475 475 a, g dbSNP:753288781
479 479 a, t dbSNP:763725349
495 495 a, g dbSNP:760388895
496 496 a, c dbSNP:775331954
498 498 a, c, t dbSNP:760002611
499 499 a, g dbSNP:774864445
500 500 g, t dbSNP:113220975
508 508 a, g dbSNP:771349222
510 510 c, g dbSNP:749874646
524 524 c, g dbSNP:773154770
535 535 -, gg dbSNP:748102093
536 536 a, g dbSNP:141872864
543 543 c, g dbSNP:374686395
549 549 a, g dbSNP:146732803
550 550 c, t dbSNP:149520370
556 556 c, t dbSNP:371253140
562 562 -, cagctggag dbSNP:769575268
565 565 c, t dbSNP:778839851
570 570 a, g dbSNP:757134177
574 574 c, g dbSNP:753868838
576 576 c, t dbSNP:763488648
578 578 c, t dbSNP:755723756
591 591 c, t dbSNP:754665816
592 592 a, g, t dbSNP:368243788
593 593 c, t dbSNP:149121402
597 597 c, t dbSNP:750850634
602 602 a, g dbSNP:765743112
605 605 c, t dbSNP:761810644
619 619 a, g dbSNP:569664227
621 621 c, g dbSNP:776685186
631 631 a, g dbSNP:768779136
647 647 a, g dbSNP:147672203
650 650 a, g dbSNP:201503266
654 654 a, c dbSNP:775819795
655 655 a, g dbSNP:770591078
669 669 a, g, t dbSNP:746547024
682 682 a, g dbSNP:779502336
683 683 c, t dbSNP:758061891
696 696 a, t dbSNP:745575196
699 699 c, t dbSNP:565245777
710 710 c, t dbSNP:757657538
730 730 a, g dbSNP:375458330
734 734 c, g, t dbSNP:756090304
736 736 c, t dbSNP:772991139
747 747 a, t dbSNP:752818834
751 751 g, t dbSNP:767553031
756 756 a, t dbSNP:200307171
758 758 c, g dbSNP:766717475
764 764 c, g, t dbSNP:753667257
789 789 c, t dbSNP:113994029
790 790 a, g dbSNP:113994028
792 792 c, g dbSNP:760641987
794 794 c, t dbSNP:775331791
803 803 c, g dbSNP:560111940
805 805 a, c, g dbSNP:773935313
812 812 a, g dbSNP:770574434
818 818 a, g dbSNP:748998115
819 819 c, g dbSNP:778177199
821 821 c, t dbSNP:376346942
838 838 a, g dbSNP:757694836
846 846 a, g dbSNP:748646310
847 847 c, t dbSNP:113994027
848 848 c, g dbSNP:781570273
864 864 c, g dbSNP:755047066
888 888 a, c, g dbSNP:151290164
890 890 a, g dbSNP:770144131
892 892 c, t dbSNP:113994030
893 893 a, g dbSNP:41288829
905 905 a, g dbSNP:769277328
906 906 c, t dbSNP:747580846
920 920 a, g dbSNP:780262752
922 922 c, t dbSNP:758593908
931 931 a, t dbSNP:750621545
936 936 c, t dbSNP:141722828
941 941 c, t dbSNP:755881182
942 942 a, g dbSNP:752440296
955 955 a, c dbSNP:143926434
962 962 c, t dbSNP:764856179
968 968 c, g dbSNP:761353843
969 969 -, ct dbSNP:775042965
970 970 c, g, t dbSNP:113994031
976 976 c, t dbSNP:182331835
977 977 c, g dbSNP:761145823
989 989 c, g, t dbSNP:149782530
993 993 a, g, t dbSNP:774281750
1001 1001 c, t dbSNP:771082359
1002 1002 c, t dbSNP:749471127
1005 1005 a, g dbSNP:777992006
1010 1010 a, g dbSNP:538217203
1027 1027 a, g dbSNP:754707878
1029 1029 a, c dbSNP:577147537
1032 1032 -, t dbSNP:767850637
1033 1033 c, t dbSNP:190848009
1036 1036 a, g dbSNP:368372393
1038 1038 c, t dbSNP:779887864
1039 1039 a, g dbSNP:758251555
1044 1044 a, g dbSNP:763088766
1065 1065 c, t dbSNP:755715511
1066 1066 a, g dbSNP:781536399
1069 1069 c, t dbSNP:780909643
1071 1071 c, g dbSNP:377339042
1080 1080 c, g, t dbSNP:78599355
1085 1085 c, t dbSNP:758877324
1090 1090 a, g dbSNP:750965977
1094 1094 a, g dbSNP:373520608
1097 1097 a, g dbSNP:761908506
1099 1099 c, t dbSNP:776661452
1102 1102 c, t dbSNP:560532019
1104 1104 c, t dbSNP:760874790
1111 1111 c, g dbSNP:184607650
1113 1113 c, t dbSNP:770898806
1115 1115 a, g dbSNP:749208137
1118 1118 a, g dbSNP:773304003
1119 1119 a, g dbSNP:769371305
1125 1125 c, t dbSNP:200954953
1136 1136 c, t dbSNP:780633076
1144 1144 c, t dbSNP:754642030
1145 1145 c, g dbSNP:746687079
1185 1185 c, g, t dbSNP:145468921
1188 1188 g, t dbSNP:768087185
1195 1195 a, g dbSNP:141025043
1205 1205 a, g dbSNP:144016401
1214 1214 g, t dbSNP:746628836
1216 1216 c, t dbSNP:779707712
1221 1221 a, g dbSNP:778375725
1224 1224 a, c, t dbSNP:779319688
1225 1225 a, g dbSNP:368168999
1232 1232 c, t dbSNP:754354365
1233 1233 c, t dbSNP:113994032
1234 1234 a, g dbSNP:113994033
1236 1236 c, g, t dbSNP:767857138
1245 1245 a, g dbSNP:375697618
1252 1252 a, c, g dbSNP:201216936
1255 1255 a, g dbSNP:113994034
1259 1259 a, g dbSNP:776461313
1266 1266 a, g dbSNP:768160292
1275 1275 c, t dbSNP:760119905
1280 1280 a, g dbSNP:775060569
1284 1284 c, t dbSNP:113994035
1285 1285 a, g dbSNP:371946487
1288 1288 c, t dbSNP:771710835
1293 1293 c, g dbSNP:745579285
1298 1298 c, t dbSNP:779229365
1303 1303 a, g dbSNP:771240893
1307 1307 c, t dbSNP:749762984
1319 1319 c, t dbSNP:144124145
1329 1329 c, g dbSNP:756150677
1336 1336 a, c, g, t dbSNP:113994036
1345 1345 c, t dbSNP:755225054
1360 1360 c, t dbSNP:755135093
1363 1363 a, g dbSNP:139134121
1365 1365 a, g dbSNP:780149885
1368 1368 c, t dbSNP:757012537
1382 1382 c, t dbSNP:753573267
1393 1393 c, g dbSNP:764012305
1394 1394 c, t dbSNP:756024921
1397 1397 c, t dbSNP:1058065
1413 1413 a, c, t dbSNP:759071387
1439 1439 a, g dbSNP:747532557
1441 1441 c, t dbSNP:369616643
1447 1447 a, g dbSNP:377146994
1454 1454 c, t dbSNP:773426946
1457 1457 c, t dbSNP:770318466
1459 1459 c, t dbSNP:748621364
1475 1475 c, t dbSNP:777297913
1485 1485 c, t dbSNP:372970925
1499 1499 a, g dbSNP:747094092
1500 1500 c, t dbSNP:759669141
1501 1501 g, t dbSNP:767391188
1502 1502 c, t dbSNP:758540260
1503 1503 a, g dbSNP:746154598
1514 1514 c, t dbSNP:777400118
1524 1524 g, t dbSNP:755934909
1541 1541 c, t dbSNP:769522881
1547 1547 c, t dbSNP:141403112
1551 1551 a, c dbSNP:768318439
1556 1556 a, g dbSNP:148810263
1557 1557 c, t dbSNP:113994038
1563 1563 c, t dbSNP:138249238
1564 1564 a, g dbSNP:751555693
1567 1567 a, g dbSNP:779387577
1575 1575 g, t dbSNP:757943510
1576 1576 a, t dbSNP:749983780
1580 1580 a, g dbSNP:764983689
1585 1585 c, g dbSNP:761628155
1595 1595 c, g dbSNP:754244635
1602 1602 a, g dbSNP:145784671
1606 1606 c, g dbSNP:761059364
1611 1611 c, t dbSNP:113994039
1612 1612 a, g dbSNP:776097463
1623 1623 c, t dbSNP:1058075
1628 1628 c, t dbSNP:1058076
1629 1629 c, t dbSNP:113994040
1634 1634 c, t dbSNP:376099446
1635 1635 a, g dbSNP:771128051
1641 1641 a, c, t dbSNP:772479767
1642 1642 a, c dbSNP:768526271
1643 1643 a, c dbSNP:746868938
1647 1647 c, g dbSNP:780050233
1653 1653 a, g dbSNP:757777425
1659 1659 a, g dbSNP:1058077
1669 1669 c, t dbSNP:749942048
1670 1670 a, g, t dbSNP:146687759
1697 1697 c, t dbSNP:753589996
1709 1709 c, t dbSNP:764517799
1713 1713 c, t dbSNP:756524864
1715 1715 a, c dbSNP:753088593
1720 1720 a, g dbSNP:768076303
1721 1721 a, g dbSNP:760017546
1723 1723 a, g dbSNP:774390579
1737 1737 c, g, t dbSNP:41288827
1738 1738 a, g dbSNP:773440658
1742 1742 -, g dbSNP:768539625
1743 1743 a, g dbSNP:768275134
1746 1746 -, c dbSNP:746849100
1746 1746 c, t dbSNP:746821092
1750 1750 a, g dbSNP:775212686
1764 1764 c, t dbSNP:772036498
1767 1767 a, g dbSNP:371845186
1770 1770 c, t dbSNP:745852708
1775 1775 c, t dbSNP:367568951
1776 1776 c, t dbSNP:779368638
1779 1779 c, g dbSNP:756863469

Target ORF information:

RefSeq Version XM_005264632
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264632

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAAGGCGG AGCTTCCCCC TGGGCCTGGG GCAGTGGGGA GGGAAATGAC CAAAGAAGAA 
AAGCTGCAGC TTCGGAAGGA AAAGAAACAG CAGAAGAAGA AACGGAAGGA AGAAAAGGGG
GCAGAACCAG AGACTGGCTC TGCTGTATCT GCAGCCCAAT GTCAAGTAGG CCCAACCAGA
GAACTGCCAG AATCGGGCAT TCAGTTGGGC ACTCCTCGGG AGAAAGTTCC AGCTGGTCGG
AGTAAGGCCG AACTTCGGGC TGAGCGTCGA GCCAAGCAGG AGGCCGAGCG GGCCCTGAAA
CAGGCAAGAA AAGGGGAACA AGGAGGACCA CCTCCTAAGG CCAGCCCCAG CACAGCTGGA
GAAACCCCCT CAGGAGTGAA GCGTCTCCCT GAGTACCCTC AGGTTGATGA CCTACTTCTG
AGAAGGCTTG TTAAAAAACC AGAGCGTCAA CAGGTTCCTA CACGAAAGGA TTATGGATCC
AAAGTCAGTC TCTTCTCTCA CCTACCCCAG TACAGCAGAC AAAACTCTCT GACCCAGTTT
ATGAGCATCC CATCCTCTGT GATCCACCCA GCCATGGTGC GACTCGGCCT GCAGTACTCC
CAGGGCCTGG TCAGTGGCTC CAATGCCCGG TGTATTGCCC TGCTTCGTGC CTTGCAGCAG
GTGATTCAGG ATTACACAAC ACCGCCTAAT GAAGAACTCT CCAGGGATCT AGTGAATAAA
CTAAAACCCT ACATGAGCTT CCTGACTCAG TGCCGTCCCC TGTCAGCGAG CATGCACAAC
GCCATCAAGT TCCTTAACAA GGAAATCACC AGTGTGGGCA GTTCCAAGCG GGAAGAGGAG
GCCAAGTCAG AACTTCGAGC AGCCATTGAT CGGTATGTGC AAGAGAAGAT TGTGCTAGCA
GCTCAGGCAA TTTCACGCTT TGCTTACCAG AAGATCAGTA ATGGAGATGT GATCCTGGTA
TATGGATGCT CATCTCTGGT ATCACGAATT CTTCAGGAGG CTTGGACAGA GGGCCGGCGG
TTTCGGGTGG TAGTGGTGGA CAGCCGGCCA TGGCTGGAAG GAAGGCACAC ACTACGTTCT
CTAGTCCATG CTGGTGTCCC AGCCTCCTAC CTGCTGATTC CTGCAGCCTC CTATGTGCTC
CCAGAGGTTT CCAAGGTGCT ATTGGGAGCT CATGCACTCT TGGCCAACGG GTCTGTGATG
TCACGGGTAG GGACAGCACA GTTAGCCCTG GTGGCTCGAG CCCATAATGT ACCAGTGCTG
GTTTGCTGTG AAACATACAA GTTCTGTGAG CGTGTGCAGA CTGATGCCTT TGTCTCTAAT
GAGCTAGATG ACCCTGATGA TCTGCAATGT AAGCGGGGAG AACATGTTGC GCTGGCTAAC
TGGCAGAACC ACGCATCCCT ACGGTTGTTG AATCTAGTCT ATGATGTGAC TCCCCCAGAG
CTTGTGGATC TGGTGATCAC GGAGCTGGGG ATGATCCCTT GCAGTTCTGT ACCTGTTGTT
CTACGAGTCA AGAGCAGTGA CCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35653
Accession Version XM_006712132.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1524bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product translation initiation factor eIF-2B subunit delta isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAAGGCGG AGCTTCCCCC TGGGCCTGGG GCAGTGGGGA GGGAAATGAC CAAAGAAGAA 
AAGCTGCAGC TTCGGAAGGA AAAGAAACAG CAGAAGAAGA AACGGAAGGA AGAAAAGGGG
GCAGAACCAG AGACTGGCTC TGCTGTATCT GCAGCCCAAT GTCAAGGCCC AACCAGAGAA
CTGCCAGAAT CGGGCATTCA GTTGGGCACT CCTCGGGAGA AAGTTCCAGC TGGTCGGAGT
AAGGCCGAAC TTCGGGCTGA GCGTCGAGCC AAGCAGGAGG CCGAGCGGGC CCTGAAACAG
GCAAGAAAAG GGGAACAAGG AGGACCACCT CCTAAGGCCA GCCCCAGCAC AGCTGGAGAA
ACCCCCTCAG GAGTGAAGCG TCTCCCTGAG TACCCTCAGG TTGATGACCT ACTTCTGAGA
AGGCTTGTTA AAAAACCAGA GCGTCAACAG GTTCCTACAC GAAAGGATTA TGGATCCAAA
GTCAGTCTCT TCTCTCACCT ACCCCAGTAC AGCAGACAAA ACTCTCTGAC CCAGTTTATG
AGCATCCCAT CCTCTGTGAT CCACCCAGCC ATGGTGCGAC TCGGCCTGCA GTACTCCCAG
GGCCTGGTCA GTGGCTCCAA TGCCCGGTGT ATTGCCCTGC TTCGTGCCTT GCAGCAGGTG
ATTCAGGATT ACACAACACC GCCTAATGAA GAACTCTCCA GGGATCTAGT GAATAAACTA
AAACCCTACA TGAGCTTCCT GACTCAGTGC CGTCCCCTGT CAGCGAGCAT GCACAACGCC
ATCAAGTTCC TTAACAAGGA AATCACCAGT GTGGGCAGTT CCAAGCGGGA AGAGGAGGCC
AAGTCAGAAC TTCGAGCAGC CATTGATCGG TATGTGCAAG AGAAGATTGT GCTAGCAGCT
CAGGCAATTT CACGCTTTGC TTACCAGAAG ATCAGTAATG GAGATGTGAT CCTGGTATAT
GGATGCTCAT CTCTGGTATC ACGAATTCTT CAGGAGGCTT GGACAGAGGG CCGGCGGTTT
CGGGTGGTAG TGGTGGACAG CCGGCCATGG CTGGAAGGAA GGCACACACT ACGTTCTCTA
GTCCATGCTG GTGTCCCAGC CTCCTACCTG CTGATTCCTG CAGCCTCCTA TGTGCTCCCA
GAGGTTTCCA AGGTGCTATT GGGAGCTCAT GCACTCTTGG CCAACGGGTC TGTGATGTCA
CGGGTAGGGA CAGCACAGTT AGCCCTGGTG GCTCGAGCCC ATAATGTACC AGTGCTGGTT
TGCTGTGAAA CATACAAGTT CTGTGAGCGT GTGCAGACTG ATGCCTTTGT CTCTAATGAG
CTAGATGACC CTGATGATCT GCAATGTAAG CGGGGAGAAC ATGTTGCGCT GGCTAACTGG
CAGAACCACG CATCCCTACG GTTGTTGAAT CTAGTCTATG ATGTGACTCC CCCAGAGCTT
GTGGATCTGG TGATCACGGA GCTGGGGATG ATCCCTTGCA GTTCTGTACC TGTTGTTCTA
CGAGTCAAGA GCAGTGACCA GTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006712195.1
CDS239..1762
Misc Feature(1)845..1741(+)
Misc Feature(2)845..1717(+)
Translation
Position Chain Variation Link
22 22 g, t dbSNP:182127717
25 25 c, g dbSNP:371924054
30 30 c, t dbSNP:575951063
35 35 c, g, t dbSNP:764607200
39 39 c, t dbSNP:760688449
40 40 c, t dbSNP:368043444
42 42 c, t dbSNP:113310794
43 43 g, t dbSNP:542570644
45 45 a, g dbSNP:759657701
47 47 a, g dbSNP:774674616
58 58 a, g dbSNP:769436465
59 59 c, t dbSNP:747983242
61 61 a, g dbSNP:781222070
79 79 a, g dbSNP:745438083
81 81 a, g dbSNP:371347853
84 84 c, g dbSNP:747012688
86 86 c, t dbSNP:575101634
89 89 c, g dbSNP:553359107
97 97 a, g dbSNP:367784417
111 111 c, g dbSNP:748747375
112 112 a, g dbSNP:577115995
116 116 c, t dbSNP:558671461
139 139 c, t dbSNP:374204525
142 142 c, t dbSNP:537135294
149 149 a, g dbSNP:568745662
180 180 c, t dbSNP:375551474
191 191 c, t dbSNP:550339577
197 197 c, t dbSNP:141681782
219 219 a, g dbSNP:568357849
229 229 a, g dbSNP:778090500
230 230 a, g dbSNP:756513459
235 235 c, t dbSNP:753104792
244 244 c, g dbSNP:190259653
254 254 c, t dbSNP:754958652
256 256 c, t dbSNP:751668695
258 258 -, c dbSNP:764709491
258 258 c, t dbSNP:766637381
260 260 c, g dbSNP:769672829
262 262 g, t dbSNP:559997020
267 267 g, t dbSNP:137963149
269 269 c, g dbSNP:747925994
270 270 a, c dbSNP:752068283
273 273 c, t dbSNP:766541364
274 274 a, g dbSNP:758587510
277 277 -, g dbSNP:761106515
277 277 a, g dbSNP:750630633
281 281 c, g dbSNP:780113294
286 286 a, g dbSNP:760328185
293 293 a, g dbSNP:775357878
298 298 a, g dbSNP:767281618
301 301 a, g dbSNP:201350814
302 302 c, g dbSNP:759503915
305 305 c, g dbSNP:144798741
318 318 a, t dbSNP:774312870
322 322 a, g dbSNP:770388291
327 327 a, g dbSNP:748820541
332 332 a, c dbSNP:368905502
334 334 -, gaagaa dbSNP:775790323
337 337 -, gaa dbSNP:772427644
337 337 a, g dbSNP:769443508
349 349 a, g dbSNP:747824895
356 356 a, g dbSNP:756565356
357 357 a, g dbSNP:781612114
358 358 g, t dbSNP:768953969
359 359 a, g dbSNP:747515908
373 373 a, t dbSNP:780733138
375 375 g, t dbSNP:139521128
378 378 c, t dbSNP:750540614
381 381 c, t dbSNP:779208304
382 382 g, t dbSNP:377209171
386 386 g, t dbSNP:754066143
389 389 a, g dbSNP:200290985
396 396 a, g dbSNP:759281615
397 397 a, g dbSNP:751451207
398 398 c, t dbSNP:766318671
406 406 c, t dbSNP:1141530
409 409 a, c dbSNP:750413412
411 411 c, t dbSNP:764777030
412 412 c, t dbSNP:547931123
428 428 a, t dbSNP:776294002
429 429 c, t dbSNP:763729559
430 430 a, g dbSNP:368615356
439 439 a, g, t dbSNP:371589780
445 445 c, t dbSNP:775992226
446 446 a, t dbSNP:530363694
450 450 -, ctc dbSNP:769733183
452 452 c, g, t dbSNP:774760656
453 453 a, g dbSNP:146262118
460 460 a, g dbSNP:770994421
468 468 c, t dbSNP:34155621
469 469 c, t dbSNP:373805172
472 472 a, t dbSNP:777985531
473 473 c, t dbSNP:756288383
481 481 a, g dbSNP:201950471
484 484 c, g dbSNP:779806044
485 485 a, g dbSNP:758233858
492 492 c, g, t dbSNP:368061442
493 493 a, g dbSNP:369882701
501 501 a, g dbSNP:753288781
505 505 a, t dbSNP:763725349
521 521 a, g dbSNP:760388895
522 522 a, c dbSNP:775331954
524 524 a, c, t dbSNP:760002611
525 525 a, g dbSNP:774864445
526 526 g, t dbSNP:113220975
534 534 a, g dbSNP:771349222
536 536 c, g dbSNP:749874646
550 550 c, g dbSNP:773154770
561 561 -, gg dbSNP:748102093
562 562 a, g dbSNP:141872864
569 569 c, g dbSNP:374686395
575 575 a, g dbSNP:146732803
576 576 c, t dbSNP:149520370
582 582 c, t dbSNP:371253140
588 588 -, cagctggag dbSNP:769575268
591 591 c, t dbSNP:778839851
596 596 a, g dbSNP:757134177
600 600 c, g dbSNP:753868838
602 602 c, t dbSNP:763488648
604 604 c, t dbSNP:755723756
617 617 c, t dbSNP:754665816
618 618 a, g, t dbSNP:368243788
619 619 c, t dbSNP:149121402
623 623 c, t dbSNP:750850634
628 628 a, g dbSNP:765743112
631 631 c, t dbSNP:761810644
645 645 a, g dbSNP:569664227
647 647 c, g dbSNP:776685186
657 657 a, g dbSNP:768779136
673 673 a, g dbSNP:147672203
676 676 a, g dbSNP:201503266
680 680 a, c dbSNP:775819795
681 681 a, g dbSNP:770591078
695 695 a, g, t dbSNP:746547024
708 708 a, g dbSNP:779502336
709 709 c, t dbSNP:758061891
722 722 a, t dbSNP:745575196
725 725 c, t dbSNP:565245777
736 736 c, t dbSNP:757657538
756 756 a, g dbSNP:375458330
760 760 c, g, t dbSNP:756090304
762 762 c, t dbSNP:772991139
773 773 a, t dbSNP:752818834
777 777 g, t dbSNP:767553031
782 782 a, t dbSNP:200307171
784 784 c, g dbSNP:766717475
790 790 c, g, t dbSNP:753667257
815 815 c, t dbSNP:113994029
816 816 a, g dbSNP:113994028
818 818 c, g dbSNP:760641987
820 820 c, t dbSNP:775331791
829 829 c, g dbSNP:560111940
831 831 a, c, g dbSNP:773935313
838 838 a, g dbSNP:770574434
844 844 a, g dbSNP:748998115
845 845 c, g dbSNP:778177199
847 847 c, t dbSNP:376346942
864 864 a, g dbSNP:757694836
872 872 a, g dbSNP:748646310
873 873 c, t dbSNP:113994027
874 874 c, g dbSNP:781570273
890 890 c, g dbSNP:755047066
914 914 a, c, g dbSNP:151290164
916 916 a, g dbSNP:770144131
918 918 c, t dbSNP:113994030
919 919 a, g dbSNP:41288829
931 931 a, g dbSNP:769277328
932 932 c, t dbSNP:747580846
946 946 a, g dbSNP:780262752
948 948 c, t dbSNP:758593908
957 957 a, t dbSNP:750621545
962 962 c, t dbSNP:141722828
967 967 c, t dbSNP:755881182
968 968 a, g dbSNP:752440296
981 981 a, c dbSNP:143926434
988 988 c, t dbSNP:764856179
994 994 c, g dbSNP:761353843
995 995 -, ct dbSNP:775042965
996 996 c, g, t dbSNP:113994031
1002 1002 c, t dbSNP:182331835
1003 1003 c, g dbSNP:761145823
1015 1015 c, g, t dbSNP:149782530
1019 1019 a, g, t dbSNP:774281750
1027 1027 c, t dbSNP:771082359
1028 1028 c, t dbSNP:749471127
1031 1031 a, g dbSNP:777992006
1036 1036 a, g dbSNP:538217203
1053 1053 a, g dbSNP:754707878
1055 1055 a, c dbSNP:577147537
1058 1058 -, t dbSNP:767850637
1059 1059 c, t dbSNP:190848009
1062 1062 a, g dbSNP:368372393
1064 1064 c, t dbSNP:779887864
1065 1065 a, g dbSNP:758251555
1070 1070 a, g dbSNP:763088766
1091 1091 c, t dbSNP:755715511
1092 1092 a, g dbSNP:781536399
1095 1095 c, t dbSNP:780909643
1097 1097 c, g dbSNP:377339042
1106 1106 c, g, t dbSNP:78599355
1111 1111 c, t dbSNP:758877324
1116 1116 a, g dbSNP:750965977
1120 1120 a, g dbSNP:373520608
1123 1123 a, g dbSNP:761908506
1125 1125 c, t dbSNP:776661452
1128 1128 c, t dbSNP:560532019
1130 1130 c, t dbSNP:760874790
1137 1137 c, g dbSNP:184607650
1139 1139 c, t dbSNP:770898806
1141 1141 a, g dbSNP:749208137
1144 1144 a, g dbSNP:773304003
1145 1145 a, g dbSNP:769371305
1151 1151 c, t dbSNP:200954953
1162 1162 c, t dbSNP:780633076
1170 1170 c, t dbSNP:754642030
1171 1171 c, g dbSNP:746687079
1211 1211 c, g, t dbSNP:145468921
1214 1214 g, t dbSNP:768087185
1221 1221 a, g dbSNP:141025043
1231 1231 a, g dbSNP:144016401
1240 1240 g, t dbSNP:746628836
1242 1242 c, t dbSNP:779707712
1247 1247 a, g dbSNP:778375725
1250 1250 a, c, t dbSNP:779319688
1251 1251 a, g dbSNP:368168999
1258 1258 c, t dbSNP:754354365
1259 1259 c, t dbSNP:113994032
1260 1260 a, g dbSNP:113994033
1262 1262 c, g, t dbSNP:767857138
1271 1271 a, g dbSNP:375697618
1278 1278 a, c, g dbSNP:201216936
1281 1281 a, g dbSNP:113994034
1285 1285 a, g dbSNP:776461313
1292 1292 a, g dbSNP:768160292
1301 1301 c, t dbSNP:760119905
1306 1306 a, g dbSNP:775060569
1310 1310 c, t dbSNP:113994035
1311 1311 a, g dbSNP:371946487
1314 1314 c, t dbSNP:771710835
1319 1319 c, g dbSNP:745579285
1324 1324 c, t dbSNP:779229365
1329 1329 a, g dbSNP:771240893
1333 1333 c, t dbSNP:749762984
1345 1345 c, t dbSNP:144124145
1355 1355 c, g dbSNP:756150677
1362 1362 a, c, g, t dbSNP:113994036
1371 1371 c, t dbSNP:755225054
1386 1386 c, t dbSNP:755135093
1389 1389 a, g dbSNP:139134121
1391 1391 a, g dbSNP:780149885
1394 1394 c, t dbSNP:757012537
1408 1408 c, t dbSNP:753573267
1419 1419 c, g dbSNP:764012305
1420 1420 c, t dbSNP:756024921
1423 1423 c, t dbSNP:1058065
1439 1439 a, c, t dbSNP:759071387
1465 1465 a, g dbSNP:747532557
1467 1467 c, t dbSNP:369616643
1473 1473 a, g dbSNP:377146994
1480 1480 c, t dbSNP:773426946
1483 1483 c, t dbSNP:770318466
1485 1485 c, t dbSNP:748621364
1501 1501 c, t dbSNP:777297913
1511 1511 c, t dbSNP:372970925
1525 1525 a, g dbSNP:747094092
1526 1526 c, t dbSNP:759669141
1527 1527 g, t dbSNP:767391188
1528 1528 c, t dbSNP:758540260
1529 1529 a, g dbSNP:746154598
1540 1540 c, t dbSNP:777400118
1550 1550 g, t dbSNP:755934909
1567 1567 c, t dbSNP:769522881
1573 1573 c, t dbSNP:141403112
1577 1577 a, c dbSNP:768318439
1582 1582 a, g dbSNP:148810263
1583 1583 c, t dbSNP:113994038
1589 1589 c, t dbSNP:138249238
1590 1590 a, g dbSNP:751555693
1593 1593 a, g dbSNP:779387577
1601 1601 g, t dbSNP:757943510
1602 1602 a, t dbSNP:749983780
1606 1606 a, g dbSNP:764983689
1611 1611 c, g dbSNP:761628155
1621 1621 c, g dbSNP:754244635
1628 1628 a, g dbSNP:145784671
1632 1632 c, g dbSNP:761059364
1637 1637 c, t dbSNP:113994039
1638 1638 a, g dbSNP:776097463
1649 1649 c, t dbSNP:1058075
1654 1654 c, t dbSNP:1058076
1655 1655 c, t dbSNP:113994040
1660 1660 c, t dbSNP:376099446
1661 1661 a, g dbSNP:771128051
1667 1667 a, c, t dbSNP:772479767
1668 1668 a, c dbSNP:768526271
1669 1669 a, c dbSNP:746868938
1673 1673 c, g dbSNP:780050233
1679 1679 a, g dbSNP:757777425
1685 1685 a, g dbSNP:1058077
1695 1695 c, t dbSNP:749942048
1696 1696 a, g, t dbSNP:146687759
1723 1723 c, t dbSNP:753589996
1735 1735 c, t dbSNP:764517799
1739 1739 c, t dbSNP:756524864
1741 1741 a, c dbSNP:753088593
1746 1746 a, g dbSNP:768076303
1747 1747 a, g dbSNP:760017546
1749 1749 a, g dbSNP:774390579
1763 1763 c, g, t dbSNP:41288827
1764 1764 a, g dbSNP:773440658
1768 1768 -, g dbSNP:768539625
1769 1769 a, g dbSNP:768275134
1772 1772 -, c dbSNP:746849100
1772 1772 c, t dbSNP:746821092
1776 1776 a, g dbSNP:775212686
1790 1790 c, t dbSNP:772036498
1793 1793 a, g dbSNP:371845186
1796 1796 c, t dbSNP:745852708
1801 1801 c, t dbSNP:367568951
1802 1802 c, t dbSNP:779368638
1805 1805 c, g dbSNP:756863469

Target ORF information:

RefSeq Version XM_006712132
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006712132

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAAGGCGG AGCTTCCCCC TGGGCCTGGG GCAGTGGGGA GGGAAATGAC CAAAGAAGAA 
AAGCTGCAGC TTCGGAAGGA AAAGAAACAG CAGAAGAAGA AACGGAAGGA AGAAAAGGGG
GCAGAACCAG AGACTGGCTC TGCTGTATCT GCAGCCCAAT GTCAAGGCCC AACCAGAGAA
CTGCCAGAAT CGGGCATTCA GTTGGGCACT CCTCGGGAGA AAGTTCCAGC TGGTCGGAGT
AAGGCCGAAC TTCGGGCTGA GCGTCGAGCC AAGCAGGAGG CCGAGCGGGC CCTGAAACAG
GCAAGAAAAG GGGAACAAGG AGGACCACCT CCTAAGGCCA GCCCCAGCAC AGCTGGAGAA
ACCCCCTCAG GAGTGAAGCG TCTCCCTGAG TACCCTCAGG TTGATGACCT ACTTCTGAGA
AGGCTTGTTA AAAAACCAGA GCGTCAACAG GTTCCTACAC GAAAGGATTA TGGATCCAAA
GTCAGTCTCT TCTCTCACCT ACCCCAGTAC AGCAGACAAA ACTCTCTGAC CCAGTTTATG
AGCATCCCAT CCTCTGTGAT CCACCCAGCC ATGGTGCGAC TCGGCCTGCA GTACTCCCAG
GGCCTGGTCA GTGGCTCCAA TGCCCGGTGT ATTGCCCTGC TTCGTGCCTT GCAGCAGGTG
ATTCAGGATT ACACAACACC GCCTAATGAA GAACTCTCCA GGGATCTAGT GAATAAACTA
AAACCCTACA TGAGCTTCCT GACTCAGTGC CGTCCCCTGT CAGCGAGCAT GCACAACGCC
ATCAAGTTCC TTAACAAGGA AATCACCAGT GTGGGCAGTT CCAAGCGGGA AGAGGAGGCC
AAGTCAGAAC TTCGAGCAGC CATTGATCGG TATGTGCAAG AGAAGATTGT GCTAGCAGCT
CAGGCAATTT CACGCTTTGC TTACCAGAAG ATCAGTAATG GAGATGTGAT CCTGGTATAT
GGATGCTCAT CTCTGGTATC ACGAATTCTT CAGGAGGCTT GGACAGAGGG CCGGCGGTTT
CGGGTGGTAG TGGTGGACAG CCGGCCATGG CTGGAAGGAA GGCACACACT ACGTTCTCTA
GTCCATGCTG GTGTCCCAGC CTCCTACCTG CTGATTCCTG CAGCCTCCTA TGTGCTCCCA
GAGGTTTCCA AGGTGCTATT GGGAGCTCAT GCACTCTTGG CCAACGGGTC TGTGATGTCA
CGGGTAGGGA CAGCACAGTT AGCCCTGGTG GCTCGAGCCC ATAATGTACC AGTGCTGGTT
TGCTGTGAAA CATACAAGTT CTGTGAGCGT GTGCAGACTG ATGCCTTTGT CTCTAATGAG
CTAGATGACC CTGATGATCT GCAATGTAAG CGGGGAGAAC ATGTTGCGCT GGCTAACTGG
CAGAACCACG CATCCCTACG GTTGTTGAAT CTAGTCTATG ATGTGACTCC CCCAGAGCTT
GTGGATCTGG TGATCACGGA GCTGGGGATG ATCCCTTGCA GTTCTGTACC TGTTGTTCTA
CGAGTCAAGA GCAGTGACCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62291
Accession Version XM_011533147.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product translation initiation factor eIF-2B subunit delta isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGTGCGAC TCGGCCTGCA GTACTCCCAG GGCCTGGTCA GTGGCTCCAA TGCCCGGTGT 
ATTGCCCTGC TTCGTGCCTT GCAGCAGGTG ATTCAGGATT ACACAACACC GCCTAATGAA
GAACTCTCCA GGGATCTAGT GAATAAACTA AAACCCTACA TGAGCTTCCT GACTCAGTGC
CGTCCCCTGT CAGCGAGCAT GCACAACGCC ATCAAGTTCC TTAACAAGGA AATCACCAGT
GTGGGCAGTT CCAAGCGGGA AGAGGAGGCC AAGTCAGAAC TTCGAGCAGC CATTGATCGG
TATGTGCAAG AGAAGATTGT GCTAGCAGCT CAGGCAATTT CACGCTTTGC TTACCAGAAG
ATCAGTAATG GAGATGTGAT CCTGGTATAT GGATGCTCAT CTCTGGTATC ACGAATTCTT
CAGGAGGCTT GGACAGAGGG CCGGCGGTTT CGGGTGGTAG TGGTGGACAG CCGGCCATGG
CTGGAAGGAA GGCACACACT ACGTTCTCTA GTCCATGCTG GTGTCCCAGC CTCCTACCTG
CTGATTCCTG CAGCCTCCTA TGTGCTCCCA GAGGTTTCCA AGGTGCTATT GGGAGCTCAT
GCACTCTTGG CCAACGGGTC TGTGATGTCA CGGGTAGGGA CAGCACAGTT AGCCCTGGTG
GCTCGAGCCC ATAATGTACC AGTGCTGGTT TGCTGTGAAA CATACAAGTT CTGTGAGCGT
GTGCAGACTG ATGCCTTTGT CTCTAATGAG CTAGATGACC CTGATGATCT GCAATGTAAG
CGGGGAGAAC ATGTTGCGCT GGCTAACTGG CAGAACCACG CATCCCTACG GTTGTTGAAT
CTAGTCTATG ATGTGACTCC CCCAGAGCTT GTGGATCTGG TGATCACGGA GCTGGGGATG
ATCCCTTGCA GTTCTGTACC TGTTGTTCTA CGAGTCAAGA GCAGTGACCA GTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011531449.1
CDS548..1501
Misc Feature(1)584..1480(+)
Misc Feature(2)584..1456(+)
Translation
Position Chain Variation Link
9 9 c, g dbSNP:190259653
19 19 c, t dbSNP:754958652
21 21 c, t dbSNP:751668695
23 23 -, c dbSNP:764709491
23 23 c, t dbSNP:766637381
25 25 c, g dbSNP:769672829
27 27 g, t dbSNP:559997020
32 32 g, t dbSNP:137963149
34 34 c, g dbSNP:747925994
35 35 a, c dbSNP:752068283
38 38 c, t dbSNP:766541364
39 39 a, g dbSNP:758587510
42 42 -, g dbSNP:761106515
42 42 a, g dbSNP:750630633
46 46 c, g dbSNP:780113294
51 51 a, g dbSNP:760328185
58 58 a, g dbSNP:775357878
63 63 a, g dbSNP:767281618
66 66 a, g dbSNP:201350814
67 67 c, g dbSNP:759503915
70 70 c, g dbSNP:144798741
83 83 a, t dbSNP:774312870
87 87 a, g dbSNP:770388291
92 92 a, g dbSNP:748820541
97 97 a, c dbSNP:368905502
99 99 -, gaagaa dbSNP:775790323
102 102 -, gaa dbSNP:772427644
102 102 a, g dbSNP:769443508
114 114 a, g dbSNP:747824895
121 121 a, g dbSNP:756565356
122 122 a, g dbSNP:781612114
123 123 g, t dbSNP:768953969
124 124 a, g dbSNP:747515908
138 138 a, t dbSNP:780733138
140 140 g, t dbSNP:139521128
143 143 c, t dbSNP:750540614
146 146 c, t dbSNP:779208304
147 147 g, t dbSNP:377209171
151 151 g, t dbSNP:754066143
154 154 a, g dbSNP:200290985
161 161 a, g dbSNP:759281615
162 162 a, g dbSNP:751451207
163 163 c, t dbSNP:766318671
171 171 c, t dbSNP:1141530
174 174 a, c dbSNP:750413412
176 176 c, t dbSNP:764777030
177 177 c, t dbSNP:547931123
193 193 a, t dbSNP:776294002
194 194 c, t dbSNP:763729559
195 195 a, g dbSNP:368615356
204 204 a, g, t dbSNP:371589780
210 210 c, t dbSNP:775992226
211 211 a, t dbSNP:530363694
215 215 -, ctc dbSNP:769733183
217 217 c, g, t dbSNP:774760656
218 218 a, g dbSNP:146262118
225 225 a, g dbSNP:770994421
233 233 c, t dbSNP:34155621
234 234 c, t dbSNP:373805172
237 237 a, t dbSNP:777985531
238 238 c, t dbSNP:756288383
246 246 a, g dbSNP:201950471
249 249 c, g dbSNP:779806044
250 250 a, g dbSNP:758233858
257 257 c, g, t dbSNP:368061442
258 258 a, g dbSNP:369882701
266 266 a, g dbSNP:753288781
270 270 a, t dbSNP:763725349
286 286 a, g dbSNP:760388895
287 287 a, c dbSNP:775331954
289 289 a, c, t dbSNP:760002611
290 290 a, g dbSNP:774864445
291 291 g, t dbSNP:113220975
299 299 a, g dbSNP:771349222
301 301 c, g dbSNP:749874646
315 315 c, g dbSNP:773154770
326 326 -, gg dbSNP:748102093
327 327 a, g dbSNP:141872864
334 334 c, g dbSNP:374686395
340 340 a, g dbSNP:146732803
341 341 c, t dbSNP:149520370
347 347 c, t dbSNP:371253140
353 353 -, cagctggag dbSNP:769575268
356 356 c, t dbSNP:778839851
361 361 a, g dbSNP:757134177
365 365 c, g dbSNP:753868838
367 367 c, t dbSNP:763488648
369 369 c, t dbSNP:755723756
382 382 c, t dbSNP:754665816
383 383 a, g, t dbSNP:368243788
384 384 c, t dbSNP:149121402
388 388 c, t dbSNP:750850634
393 393 a, g dbSNP:765743112
396 396 c, t dbSNP:761810644
410 410 a, g dbSNP:569664227
412 412 c, g dbSNP:776685186
422 422 a, g dbSNP:768779136
438 438 a, g dbSNP:147672203
441 441 a, g dbSNP:201503266
445 445 a, c dbSNP:775819795
446 446 a, g dbSNP:770591078
460 460 a, g, t dbSNP:746547024
473 473 a, g dbSNP:779502336
474 474 c, t dbSNP:758061891
487 487 a, t dbSNP:745575196
490 490 c, t dbSNP:565245777
501 501 c, t dbSNP:757657538
521 521 a, g dbSNP:375458330
525 525 c, g, t dbSNP:756090304
527 527 c, t dbSNP:772991139
538 538 a, t dbSNP:752818834
542 542 g, t dbSNP:767553031
554 554 c, t dbSNP:113994029
555 555 a, g dbSNP:113994028
557 557 c, g dbSNP:760641987
559 559 c, t dbSNP:775331791
568 568 c, g dbSNP:560111940
570 570 a, c, g dbSNP:773935313
577 577 a, g dbSNP:770574434
583 583 a, g dbSNP:748998115
584 584 c, g dbSNP:778177199
586 586 c, t dbSNP:376346942
603 603 a, g dbSNP:757694836
611 611 a, g dbSNP:748646310
612 612 c, t dbSNP:113994027
613 613 c, g dbSNP:781570273
629 629 c, g dbSNP:755047066
653 653 a, c, g dbSNP:151290164
655 655 a, g dbSNP:770144131
657 657 c, t dbSNP:113994030
658 658 a, g dbSNP:41288829
670 670 a, g dbSNP:769277328
671 671 c, t dbSNP:747580846
685 685 a, g dbSNP:780262752
687 687 c, t dbSNP:758593908
696 696 a, t dbSNP:750621545
701 701 c, t dbSNP:141722828
706 706 c, t dbSNP:755881182
707 707 a, g dbSNP:752440296
720 720 a, c dbSNP:143926434
727 727 c, t dbSNP:764856179
733 733 c, g dbSNP:761353843
734 734 -, ct dbSNP:775042965
735 735 c, g, t dbSNP:113994031
741 741 c, t dbSNP:182331835
742 742 c, g dbSNP:761145823
754 754 c, g, t dbSNP:149782530
758 758 a, g, t dbSNP:774281750
766 766 c, t dbSNP:771082359
767 767 c, t dbSNP:749471127
770 770 a, g dbSNP:777992006
775 775 a, g dbSNP:538217203
792 792 a, g dbSNP:754707878
794 794 a, c dbSNP:577147537
797 797 -, t dbSNP:767850637
798 798 c, t dbSNP:190848009
801 801 a, g dbSNP:368372393
803 803 c, t dbSNP:779887864
804 804 a, g dbSNP:758251555
809 809 a, g dbSNP:763088766
830 830 c, t dbSNP:755715511
831 831 a, g dbSNP:781536399
834 834 c, t dbSNP:780909643
836 836 c, g dbSNP:377339042
845 845 c, g, t dbSNP:78599355
850 850 c, t dbSNP:758877324
855 855 a, g dbSNP:750965977
859 859 a, g dbSNP:373520608
862 862 a, g dbSNP:761908506
864 864 c, t dbSNP:776661452
867 867 c, t dbSNP:560532019
869 869 c, t dbSNP:760874790
876 876 c, g dbSNP:184607650
878 878 c, t dbSNP:770898806
880 880 a, g dbSNP:749208137
883 883 a, g dbSNP:773304003
884 884 a, g dbSNP:769371305
890 890 c, t dbSNP:200954953
901 901 c, t dbSNP:780633076
909 909 c, t dbSNP:754642030
910 910 c, g dbSNP:746687079
950 950 c, g, t dbSNP:145468921
953 953 g, t dbSNP:768087185
960 960 a, g dbSNP:141025043
970 970 a, g dbSNP:144016401
979 979 g, t dbSNP:746628836
981 981 c, t dbSNP:779707712
986 986 a, g dbSNP:778375725
989 989 a, c, t dbSNP:779319688
990 990 a, g dbSNP:368168999
997 997 c, t dbSNP:754354365
998 998 c, t dbSNP:113994032
999 999 a, g dbSNP:113994033
1001 1001 c, g, t dbSNP:767857138
1010 1010 a, g dbSNP:375697618
1017 1017 a, c, g dbSNP:201216936
1020 1020 a, g dbSNP:113994034
1024 1024 a, g dbSNP:776461313
1031 1031 a, g dbSNP:768160292
1040 1040 c, t dbSNP:760119905
1045 1045 a, g dbSNP:775060569
1049 1049 c, t dbSNP:113994035
1050 1050 a, g dbSNP:371946487
1053 1053 c, t dbSNP:771710835
1058 1058 c, g dbSNP:745579285
1063 1063 c, t dbSNP:779229365
1068 1068 a, g dbSNP:771240893
1072 1072 c, t dbSNP:749762984
1084 1084 c, t dbSNP:144124145
1094 1094 c, g dbSNP:756150677
1101 1101 a, c, g, t dbSNP:113994036
1110 1110 c, t dbSNP:755225054
1125 1125 c, t dbSNP:755135093
1128 1128 a, g dbSNP:139134121
1130 1130 a, g dbSNP:780149885
1133 1133 c, t dbSNP:757012537
1147 1147 c, t dbSNP:753573267
1158 1158 c, g dbSNP:764012305
1159 1159 c, t dbSNP:756024921
1162 1162 c, t dbSNP:1058065
1178 1178 a, c, t dbSNP:759071387
1204 1204 a, g dbSNP:747532557
1206 1206 c, t dbSNP:369616643
1212 1212 a, g dbSNP:377146994
1219 1219 c, t dbSNP:773426946
1222 1222 c, t dbSNP:770318466
1224 1224 c, t dbSNP:748621364
1240 1240 c, t dbSNP:777297913
1250 1250 c, t dbSNP:372970925
1264 1264 a, g dbSNP:747094092
1265 1265 c, t dbSNP:759669141
1266 1266 g, t dbSNP:767391188
1267 1267 c, t dbSNP:758540260
1268 1268 a, g dbSNP:746154598
1279 1279 c, t dbSNP:777400118
1289 1289 g, t dbSNP:755934909
1306 1306 c, t dbSNP:769522881
1312 1312 c, t dbSNP:141403112
1316 1316 a, c dbSNP:768318439
1321 1321 a, g dbSNP:148810263
1322 1322 c, t dbSNP:113994038
1328 1328 c, t dbSNP:138249238
1329 1329 a, g dbSNP:751555693
1332 1332 a, g dbSNP:779387577
1340 1340 g, t dbSNP:757943510
1341 1341 a, t dbSNP:749983780
1345 1345 a, g dbSNP:764983689
1350 1350 c, g dbSNP:761628155
1360 1360 c, g dbSNP:754244635
1367 1367 a, g dbSNP:145784671
1371 1371 c, g dbSNP:761059364
1376 1376 c, t dbSNP:113994039
1377 1377 a, g dbSNP:776097463
1388 1388 c, t dbSNP:1058075
1393 1393 c, t dbSNP:1058076
1394 1394 c, t dbSNP:113994040
1399 1399 c, t dbSNP:376099446
1400 1400 a, g dbSNP:771128051
1406 1406 a, c, t dbSNP:772479767
1407 1407 a, c dbSNP:768526271
1408 1408 a, c dbSNP:746868938
1412 1412 c, g dbSNP:780050233
1418 1418 a, g dbSNP:757777425
1424 1424 a, g dbSNP:1058077
1434 1434 c, t dbSNP:749942048
1435 1435 a, g, t dbSNP:146687759
1462 1462 c, t dbSNP:753589996
1474 1474 c, t dbSNP:764517799
1478 1478 c, t dbSNP:756524864
1480 1480 a, c dbSNP:753088593
1485 1485 a, g dbSNP:768076303
1486 1486 a, g dbSNP:760017546
1488 1488 a, g dbSNP:774390579
1502 1502 c, g, t dbSNP:41288827
1503 1503 a, g dbSNP:773440658
1507 1507 -, g dbSNP:768539625
1508 1508 a, g dbSNP:768275134
1511 1511 -, c dbSNP:746849100
1511 1511 c, t dbSNP:746821092
1515 1515 a, g dbSNP:775212686
1529 1529 c, t dbSNP:772036498
1532 1532 a, g dbSNP:371845186
1535 1535 c, t dbSNP:745852708
1540 1540 c, t dbSNP:367568951
1541 1541 c, t dbSNP:779368638
1544 1544 c, g dbSNP:756863469

Target ORF information:

RefSeq Version XM_011533147
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011533147

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGTGCGAC TCGGCCTGCA GTACTCCCAG GGCCTGGTCA GTGGCTCCAA TGCCCGGTGT 
ATTGCCCTGC TTCGTGCCTT GCAGCAGGTG ATTCAGGATT ACACAACACC GCCTAATGAA
GAACTCTCCA GGGATCTAGT GAATAAACTA AAACCCTACA TGAGCTTCCT GACTCAGTGC
CGTCCCCTGT CAGCGAGCAT GCACAACGCC ATCAAGTTCC TTAACAAGGA AATCACCAGT
GTGGGCAGTT CCAAGCGGGA AGAGGAGGCC AAGTCAGAAC TTCGAGCAGC CATTGATCGG
TATGTGCAAG AGAAGATTGT GCTAGCAGCT CAGGCAATTT CACGCTTTGC TTACCAGAAG
ATCAGTAATG GAGATGTGAT CCTGGTATAT GGATGCTCAT CTCTGGTATC ACGAATTCTT
CAGGAGGCTT GGACAGAGGG CCGGCGGTTT CGGGTGGTAG TGGTGGACAG CCGGCCATGG
CTGGAAGGAA GGCACACACT ACGTTCTCTA GTCCATGCTG GTGTCCCAGC CTCCTACCTG
CTGATTCCTG CAGCCTCCTA TGTGCTCCCA GAGGTTTCCA AGGTGCTATT GGGAGCTCAT
GCACTCTTGG CCAACGGGTC TGTGATGTCA CGGGTAGGGA CAGCACAGTT AGCCCTGGTG
GCTCGAGCCC ATAATGTACC AGTGCTGGTT TGCTGTGAAA CATACAAGTT CTGTGAGCGT
GTGCAGACTG ATGCCTTTGT CTCTAATGAG CTAGATGACC CTGATGATCT GCAATGTAAG
CGGGGAGAAC ATGTTGCGCT GGCTAACTGG CAGAACCACG CATCCCTACG GTTGTTGAAT
CTAGTCTATG ATGTGACTCC CCCAGAGCTT GTGGATCTGG TGATCACGGA GCTGGGGATG
ATCCCTTGCA GTTCTGTACC TGTTGTTCTA CGAGTCAAGA GCAGTGACCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22903
Accession Version NM_015636.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1569bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product translation initiation factor eIF-2B subunit delta isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AC074117.7. On Oct 21, 2005 this sequence version replaced gi:26986531. Summary: Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (3) differs in the 5' UTR and coding region, compared to variant 1. The resulting protein (isoform 3, also known as the short isoform), is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ011305.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCTGCTG TGGCCGTGGC TGTTCGCGAG GACTCGGGAT CCGGGATGAA GGCGGAGCTT 
CCCCCTGGGC CTGGGGCAGT GGGGAGGGAA ATGACCAAAG AAGAAAAGCT GCAGCTTCGG
AAGGAAAAGA AACAGCAGAA GAAGAAACGG AAGGAAGAAA AGGGGGCAGA ACCAGAGACT
GGCTCTGCTG TATCTGCAGC CCAATGTCAA GGCCCAACCA GAGAACTGCC AGAATCGGGC
ATTCAGTTGG GCACTCCTCG GGAGAAAGTT CCAGCTGGTC GGAGTAAGGC CGAACTTCGG
GCTGAGCGTC GAGCCAAGCA GGAGGCCGAG CGGGCCCTGA AACAGGCAAG AAAAGGGGAA
CAAGGAGGAC CACCTCCTAA GGCCAGCCCC AGCACAGCTG GAGAAACCCC CTCAGGAGTG
AAGCGTCTCC CTGAGTACCC TCAGGTTGAT GACCTACTTC TGAGAAGGCT TGTTAAAAAA
CCAGAGCGTC AACAGGTTCC TACACGAAAG GATTATGGAT CCAAAGTCAG TCTCTTCTCT
CACCTACCCC AGTACAGCAG ACAAAACTCT CTGACCCAGT TTATGAGCAT CCCATCCTCT
GTGATCCACC CAGCCATGGT GCGACTCGGC CTGCAGTACT CCCAGGGCCT GGTCAGTGGC
TCCAATGCCC GGTGTATTGC CCTGCTTCGT GCCTTGCAGC AGGTGATTCA GGATTACACA
ACACCGCCTA ATGAAGAACT CTCCAGGGAT CTAGTGAATA AACTAAAACC CTACATGAGC
TTCCTGACTC AGTGCCGTCC CCTGTCAGCG AGCATGCACA ACGCCATCAA GTTCCTTAAC
AAGGAAATCA CCAGTGTGGG CAGTTCCAAG CGGGAAGAGG AGGCCAAGTC AGAACTTCGA
GCAGCCATTG ATCGGTATGT GCAAGAGAAG ATTGTGCTAG CAGCTCAGGC AATTTCACGC
TTTGCTTACC AGAAGATCAG TAATGGAGAT GTGATCCTGG TATATGGATG CTCATCTCTG
GTATCACGAA TTCTTCAGGA GGCTTGGACA GAGGGCCGGC GGTTTCGGGT GGTAGTGGTG
GACAGCCGGC CATGGCTGGA AGGAAGGCAC ACACTACGTT CTCTAGTCCA TGCTGGTGTC
CCAGCCTCCT ACCTGCTGAT TCCTGCAGCC TCCTATGTGC TCCCAGAGGT TTCCAAGGTG
CTATTGGGAG CTCATGCACT CTTGGCCAAC GGGTCTGTGA TGTCACGGGT AGGGACAGCA
CAGTTAGCCC TGGTGGCTCG AGCCCATAAT GTACCAGTGC TGGTTTGCTG TGAAACATAC
AAGTTCTGTG AGCGTGTGCA GACTGATGCC TTTGTCTCTA ATGAGCTAGA TGACCCTGAT
GATCTGCAAT GTAAGCGGGG AGAACATGTT GCGCTGGCTA ACTGGCAGAA CCACGCATCC
CTACGGTTGT TGAATCTAGT CTATGATGTG ACTCCCCCAG AGCTTGTGGA TCTGGTGATC
ACGGAGCTGG GGATGATCCC TTGCAGTTCT GTACCTGTTG TTCTACGAGT CAAGAGCAGT
GACCAGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_056451.3
CDS144..1712
Misc Feature(1)1..1(+)
Misc Feature(2)126..126(+)
Misc Feature(3)147..149(+)
Misc Feature(4)396..398(+)
Misc Feature(5)795..1691(+)
Misc Feature(6)795..1667(+)
Exon (1)1..174
Gene:EIF2B4
Gene Synonym:
Exon (2)175..218
Gene:EIF2B4
Gene Synonym:
Exon (3)219..354
Gene:EIF2B4
Gene Synonym:
Exon (4)355..558
Gene:EIF2B4
Gene Synonym:
Exon (5)559..638
Gene:EIF2B4
Gene Synonym:
Exon (6)639..730
Gene:EIF2B4
Gene Synonym:
Exon (7)731..845
Gene:EIF2B4
Gene Synonym:
Exon (8)846..922
Gene:EIF2B4
Gene Synonym:
Exon (9)923..1025
Gene:EIF2B4
Gene Synonym:
Exon (10)1026..1153
Gene:EIF2B4
Gene Synonym:
Exon (11)1154..1331
Gene:EIF2B4
Gene Synonym:
Exon (12)1332..1512
Gene:EIF2B4
Gene Synonym:
Exon (13)1513..1760
Gene:EIF2B4
Gene Synonym:
Translation
Position Chain Variation Link
11 11 g, t dbSNP:561557212
13 13 a, c dbSNP:540244387
27 27 c, t dbSNP:528039279
28 28 c, g dbSNP:761436350
42 42 a, g dbSNP:547438085
94 94 c, t dbSNP:564213305
100 100 c, g dbSNP:750144681
150 150 g, t dbSNP:182127717
153 153 c, g dbSNP:371924054
158 158 c, t dbSNP:575951063
163 163 c, g, t dbSNP:764607200
167 167 c, t dbSNP:760688449
168 168 c, t dbSNP:368043444
170 170 c, t dbSNP:113310794
171 171 g, t dbSNP:542570644
173 173 a, g dbSNP:759657701
179 179 a, g dbSNP:778090500
180 180 a, g dbSNP:756513459
185 185 c, t dbSNP:753104792
194 194 c, g dbSNP:190259653
204 204 c, t dbSNP:754958652
206 206 c, t dbSNP:751668695
208 208 -, c dbSNP:764709491
208 208 c, t dbSNP:766637381
210 210 c, g dbSNP:769672829
212 212 g, t dbSNP:559997020
217 217 g, t dbSNP:137963149
219 219 c, g dbSNP:747925994
220 220 a, c dbSNP:752068283
223 223 c, t dbSNP:766541364
224 224 a, g dbSNP:758587510
227 227 -, g dbSNP:761106515
227 227 a, g dbSNP:750630633
231 231 c, g dbSNP:780113294
236 236 a, g dbSNP:760328185
243 243 a, g dbSNP:775357878
248 248 a, g dbSNP:767281618
251 251 a, g dbSNP:201350814
252 252 c, g dbSNP:759503915
255 255 c, g dbSNP:144798741
268 268 a, t dbSNP:774312870
272 272 a, g dbSNP:770388291
277 277 a, g dbSNP:748820541
282 282 a, c dbSNP:368905502
284 284 -, gaagaa dbSNP:775790323
287 287 -, gaa dbSNP:772427644
287 287 a, g dbSNP:769443508
299 299 a, g dbSNP:747824895
306 306 a, g dbSNP:756565356
307 307 a, g dbSNP:781612114
308 308 g, t dbSNP:768953969
309 309 a, g dbSNP:747515908
323 323 a, t dbSNP:780733138
325 325 g, t dbSNP:139521128
328 328 c, t dbSNP:750540614
331 331 c, t dbSNP:779208304
332 332 g, t dbSNP:377209171
336 336 g, t dbSNP:754066143
339 339 a, g dbSNP:200290985
346 346 a, g dbSNP:759281615
347 347 a, g dbSNP:751451207
348 348 c, t dbSNP:766318671
356 356 c, t dbSNP:1141530
359 359 a, c dbSNP:750413412
361 361 c, t dbSNP:764777030
362 362 c, t dbSNP:547931123
378 378 a, t dbSNP:776294002
379 379 c, t dbSNP:763729559
380 380 a, g dbSNP:368615356
389 389 a, g, t dbSNP:371589780
395 395 c, t dbSNP:775992226
396 396 a, t dbSNP:530363694
400 400 -, ctc dbSNP:769733183
402 402 c, g, t dbSNP:774760656
403 403 a, g dbSNP:146262118
410 410 a, g dbSNP:770994421
418 418 c, t dbSNP:34155621
419 419 c, t dbSNP:373805172
422 422 a, t dbSNP:777985531
423 423 c, t dbSNP:756288383
431 431 a, g dbSNP:201950471
434 434 c, g dbSNP:779806044
435 435 a, g dbSNP:758233858
442 442 c, g, t dbSNP:368061442
443 443 a, g dbSNP:369882701
451 451 a, g dbSNP:753288781
455 455 a, t dbSNP:763725349
471 471 a, g dbSNP:760388895
472 472 a, c dbSNP:775331954
474 474 a, c, t dbSNP:760002611
475 475 a, g dbSNP:774864445
476 476 g, t dbSNP:113220975
484 484 a, g dbSNP:771349222
486 486 c, g dbSNP:749874646
500 500 c, g dbSNP:773154770
511 511 -, gg dbSNP:748102093
512 512 a, g dbSNP:141872864
519 519 c, g dbSNP:374686395
525 525 a, g dbSNP:146732803
526 526 c, t dbSNP:149520370
532 532 c, t dbSNP:371253140
538 538 -, cagctggag dbSNP:769575268
541 541 c, t dbSNP:778839851
546 546 a, g dbSNP:757134177
550 550 c, g dbSNP:753868838
552 552 c, t dbSNP:763488648
554 554 c, t dbSNP:755723756
567 567 c, t dbSNP:754665816
568 568 a, g, t dbSNP:368243788
569 569 c, t dbSNP:149121402
573 573 c, t dbSNP:750850634
578 578 a, g dbSNP:765743112
581 581 c, t dbSNP:761810644
595 595 a, g dbSNP:569664227
597 597 c, g dbSNP:776685186
607 607 a, g dbSNP:768779136
623 623 a, g dbSNP:147672203
626 626 a, g dbSNP:201503266
630 630 a, c dbSNP:775819795
631 631 a, g dbSNP:770591078
645 645 a, g, t dbSNP:746547024
658 658 a, g dbSNP:779502336
659 659 c, t dbSNP:758061891
672 672 a, t dbSNP:745575196
675 675 c, t dbSNP:565245777
686 686 c, t dbSNP:757657538
706 706 a, g dbSNP:375458330
710 710 c, g, t dbSNP:756090304
712 712 c, t dbSNP:772991139
723 723 a, t dbSNP:752818834
727 727 g, t dbSNP:767553031
732 732 a, t dbSNP:200307171
734 734 c, g dbSNP:766717475
740 740 c, g, t dbSNP:753667257
765 765 c, t dbSNP:113994029
766 766 a, g dbSNP:113994028
768 768 c, g dbSNP:760641987
770 770 c, t dbSNP:775331791
779 779 c, g dbSNP:560111940
781 781 a, c, g dbSNP:773935313
788 788 a, g dbSNP:770574434
794 794 a, g dbSNP:748998115
795 795 c, g dbSNP:778177199
797 797 c, t dbSNP:376346942
814 814 a, g dbSNP:757694836
822 822 a, g dbSNP:748646310
823 823 c, t dbSNP:113994027
824 824 c, g dbSNP:781570273
840 840 c, g dbSNP:755047066
864 864 a, c, g dbSNP:151290164
866 866 a, g dbSNP:770144131
868 868 c, t dbSNP:113994030
869 869 a, g dbSNP:41288829
881 881 a, g dbSNP:769277328
882 882 c, t dbSNP:747580846
896 896 a, g dbSNP:780262752
898 898 c, t dbSNP:758593908
907 907 a, t dbSNP:750621545
912 912 c, t dbSNP:141722828
917 917 c, t dbSNP:755881182
918 918 a, g dbSNP:752440296
931 931 a, c dbSNP:143926434
938 938 c, t dbSNP:764856179
944 944 c, g dbSNP:761353843
945 945 -, ct dbSNP:775042965
946 946 c, g, t dbSNP:113994031
952 952 c, t dbSNP:182331835
953 953 c, g dbSNP:761145823
965 965 c, g, t dbSNP:149782530
969 969 a, g, t dbSNP:774281750
977 977 c, t dbSNP:771082359
978 978 c, t dbSNP:749471127
981 981 a, g dbSNP:777992006
986 986 a, g dbSNP:538217203
1003 1003 a, g dbSNP:754707878
1005 1005 a, c dbSNP:577147537
1008 1008 -, t dbSNP:767850637
1009 1009 c, t dbSNP:190848009
1012 1012 a, g dbSNP:368372393
1014 1014 c, t dbSNP:779887864
1015 1015 a, g dbSNP:758251555
1020 1020 a, g dbSNP:763088766
1041 1041 c, t dbSNP:755715511
1042 1042 a, g dbSNP:781536399
1045 1045 c, t dbSNP:780909643
1047 1047 c, g dbSNP:377339042
1056 1056 c, g, t dbSNP:78599355
1061 1061 c, t dbSNP:758877324
1066 1066 a, g dbSNP:750965977
1070 1070 a, g dbSNP:373520608
1073 1073 a, g dbSNP:761908506
1075 1075 c, t dbSNP:776661452
1078 1078 c, t dbSNP:560532019
1080 1080 c, t dbSNP:760874790
1087 1087 c, g dbSNP:184607650
1089 1089 c, t dbSNP:770898806
1091 1091 a, g dbSNP:749208137
1094 1094 a, g dbSNP:773304003
1095 1095 a, g dbSNP:769371305
1101 1101 c, t dbSNP:200954953
1112 1112 c, t dbSNP:780633076
1120 1120 c, t dbSNP:754642030
1121 1121 c, g dbSNP:746687079
1161 1161 c, g, t dbSNP:145468921
1164 1164 g, t dbSNP:768087185
1171 1171 a, g dbSNP:141025043
1181 1181 a, g dbSNP:144016401
1190 1190 g, t dbSNP:746628836
1192 1192 c, t dbSNP:779707712
1197 1197 a, g dbSNP:778375725
1200 1200 a, c, t dbSNP:779319688
1201 1201 a, g dbSNP:368168999
1208 1208 c, t dbSNP:754354365
1209 1209 c, t dbSNP:113994032
1210 1210 a, g dbSNP:113994033
1212 1212 c, g, t dbSNP:767857138
1221 1221 a, g dbSNP:375697618
1228 1228 a, c, g dbSNP:201216936
1231 1231 a, g dbSNP:113994034
1235 1235 a, g dbSNP:776461313
1242 1242 a, g dbSNP:768160292
1251 1251 c, t dbSNP:760119905
1256 1256 a, g dbSNP:775060569
1260 1260 c, t dbSNP:113994035
1261 1261 a, g dbSNP:371946487
1264 1264 c, t dbSNP:771710835
1269 1269 c, g dbSNP:745579285
1274 1274 c, t dbSNP:779229365
1279 1279 a, g dbSNP:771240893
1283 1283 c, t dbSNP:749762984
1295 1295 c, t dbSNP:144124145
1305 1305 c, g dbSNP:756150677
1312 1312 a, c, g, t dbSNP:113994036
1321 1321 c, t dbSNP:755225054
1336 1336 c, t dbSNP:755135093
1339 1339 a, g dbSNP:139134121
1341 1341 a, g dbSNP:780149885
1344 1344 c, t dbSNP:757012537
1358 1358 c, t dbSNP:753573267
1369 1369 c, g dbSNP:764012305
1370 1370 c, t dbSNP:756024921
1373 1373 c, t dbSNP:1058065
1389 1389 a, c, t dbSNP:759071387
1415 1415 a, g dbSNP:747532557
1417 1417 c, t dbSNP:369616643
1423 1423 a, g dbSNP:377146994
1430 1430 c, t dbSNP:773426946
1433 1433 c, t dbSNP:770318466
1435 1435 c, t dbSNP:748621364
1451 1451 c, t dbSNP:777297913
1461 1461 c, t dbSNP:372970925
1475 1475 a, g dbSNP:747094092
1476 1476 c, t dbSNP:759669141
1477 1477 g, t dbSNP:767391188
1478 1478 c, t dbSNP:758540260
1479 1479 a, g dbSNP:746154598
1490 1490 c, t dbSNP:777400118
1500 1500 g, t dbSNP:755934909
1517 1517 c, t dbSNP:769522881
1523 1523 c, t dbSNP:141403112
1527 1527 a, c dbSNP:768318439
1532 1532 a, g dbSNP:148810263
1533 1533 c, t dbSNP:113994038
1539 1539 c, t dbSNP:138249238
1540 1540 a, g dbSNP:751555693
1543 1543 a, g dbSNP:779387577
1551 1551 g, t dbSNP:757943510
1552 1552 a, t dbSNP:749983780
1556 1556 a, g dbSNP:764983689
1561 1561 c, g dbSNP:761628155
1571 1571 c, g dbSNP:754244635
1578 1578 a, g dbSNP:145784671
1582 1582 c, g dbSNP:761059364
1587 1587 c, t dbSNP:113994039
1588 1588 a, g dbSNP:776097463
1599 1599 c, t dbSNP:1058075
1604 1604 c, t dbSNP:1058076
1605 1605 c, t dbSNP:113994040
1610 1610 c, t dbSNP:376099446
1611 1611 a, g dbSNP:771128051
1617 1617 a, c, t dbSNP:772479767
1618 1618 a, c dbSNP:768526271
1619 1619 a, c dbSNP:746868938
1623 1623 c, g dbSNP:780050233
1629 1629 a, g dbSNP:757777425
1635 1635 a, g dbSNP:1058077
1645 1645 c, t dbSNP:749942048
1646 1646 a, g, t dbSNP:146687759
1673 1673 c, t dbSNP:753589996
1685 1685 c, t dbSNP:764517799
1689 1689 c, t dbSNP:756524864
1691 1691 a, c dbSNP:753088593
1696 1696 a, g dbSNP:768076303
1697 1697 a, g dbSNP:760017546
1699 1699 a, g dbSNP:774390579
1713 1713 c, g, t dbSNP:41288827
1714 1714 a, g dbSNP:773440658
1718 1718 -, g dbSNP:768539625
1719 1719 a, g dbSNP:768275134
1722 1722 -, c dbSNP:746849100
1722 1722 c, t dbSNP:746821092
1726 1726 a, g dbSNP:775212686
1740 1740 c, t dbSNP:772036498
1743 1743 a, g dbSNP:371845186
1746 1746 c, t dbSNP:745852708
1751 1751 c, t dbSNP:367568951
1752 1752 c, t dbSNP:779368638
1755 1755 c, g dbSNP:756863469

Target ORF information:

RefSeq Version NM_015636
Organism Homo sapiens (human)
Definition Homo sapiens eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_015636

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCTGCTG TGGCCGTGGC TGTTCGCGAG GACTCGGGAT CCGGGATGAA GGCGGAGCTT 
CCCCCTGGGC CTGGGGCAGT GGGGAGGGAA ATGACCAAAG AAGAAAAGCT GCAGCTTCGG
AAGGAAAAGA AACAGCAGAA GAAGAAACGG AAGGAAGAAA AGGGGGCAGA ACCAGAGACT
GGCTCTGCTG TATCTGCAGC CCAATGTCAA GGCCCAACCA GAGAACTGCC AGAATCGGGC
ATTCAGTTGG GCACTCCTCG GGAGAAAGTT CCAGCTGGTC GGAGTAAGGC CGAACTTCGG
GCTGAGCGTC GAGCCAAGCA GGAGGCCGAG CGGGCCCTGA AACAGGCAAG AAAAGGGGAA
CAAGGAGGAC CACCTCCTAA GGCCAGCCCC AGCACAGCTG GAGAAACCCC CTCAGGAGTG
AAGCGTCTCC CTGAGTACCC TCAGGTTGAT GACCTACTTC TGAGAAGGCT TGTTAAAAAA
CCAGAGCGTC AACAGGTTCC TACACGAAAG GATTATGGAT CCAAAGTCAG TCTCTTCTCT
CACCTACCCC AGTACAGCAG ACAAAACTCT CTGACCCAGT TTATGAGCAT CCCATCCTCT
GTGATCCACC CAGCCATGGT GCGACTCGGC CTGCAGTACT CCCAGGGCCT GGTCAGTGGC
TCCAATGCCC GGTGTATTGC CCTGCTTCGT GCCTTGCAGC AGGTGATTCA GGATTACACA
ACACCGCCTA ATGAAGAACT CTCCAGGGAT CTAGTGAATA AACTAAAACC CTACATGAGC
TTCCTGACTC AGTGCCGTCC CCTGTCAGCG AGCATGCACA ACGCCATCAA GTTCCTTAAC
AAGGAAATCA CCAGTGTGGG CAGTTCCAAG CGGGAAGAGG AGGCCAAGTC AGAACTTCGA
GCAGCCATTG ATCGGTATGT GCAAGAGAAG ATTGTGCTAG CAGCTCAGGC AATTTCACGC
TTTGCTTACC AGAAGATCAG TAATGGAGAT GTGATCCTGG TATATGGATG CTCATCTCTG
GTATCACGAA TTCTTCAGGA GGCTTGGACA GAGGGCCGGC GGTTTCGGGT GGTAGTGGTG
GACAGCCGGC CATGGCTGGA AGGAAGGCAC ACACTACGTT CTCTAGTCCA TGCTGGTGTC
CCAGCCTCCT ACCTGCTGAT TCCTGCAGCC TCCTATGTGC TCCCAGAGGT TTCCAAGGTG
CTATTGGGAG CTCATGCACT CTTGGCCAAC GGGTCTGTGA TGTCACGGGT AGGGACAGCA
CAGTTAGCCC TGGTGGCTCG AGCCCATAAT GTACCAGTGC TGGTTTGCTG TGAAACATAC
AAGTTCTGTG AGCGTGTGCA GACTGATGCC TTTGTCTCTA ATGAGCTAGA TGACCCTGAT
GATCTGCAAT GTAAGCGGGG AGAACATGTT GCGCTGGCTA ACTGGCAGAA CCACGCATCC
CTACGGTTGT TGAATCTAGT CTATGATGTG ACTCCCCCAG AGCTTGTGGA TCTGGTGATC
ACGGAGCTGG GGATGATCCC TTGCAGTTCT GTACCTGTTG TTCTACGAGT CAAGAGCAGT
GACCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22714
Accession Version NM_001034116.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1572bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu22714D_COA.pdf (pdf)
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product translation initiation factor eIF-2B subunit delta isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC074117.7. This sequence is a reference standard in the RefSeqGene project. Summary: Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) differs in the 5' UTR and coding region, and also uses an alternate in-frame segment in the 5' coding region, compare to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF112207.1, BC091502.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCTGCTG TGGCCGTGGC TGTTCGCGAG GACTCGGGAT CCGGGATGAA GGCGGAGCTT 
CCCCCTGGGC CTGGGGCAGT GGGGAGGGAA ATGACCAAAG AAGAAAAGCT GCAGCTTCGG
AAGGAAAAGA AACAGCAGAA GAAGAAACGG AAGGAAGAAA AGGGGGCAGA ACCAGAGACT
GGCTCTGCTG TATCTGCAGC CCAATGTCAA GTAGGCCCAA CCAGAGAACT GCCAGAATCG
GGCATTCAGT TGGGCACTCC TCGGGAGAAA GTTCCAGCTG GTCGGAGTAA GGCCGAACTT
CGGGCTGAGC GTCGAGCCAA GCAGGAGGCC GAGCGGGCCC TGAAACAGGC AAGAAAAGGG
GAACAAGGAG GACCACCTCC TAAGGCCAGC CCCAGCACAG CTGGAGAAAC CCCCTCAGGA
GTGAAGCGTC TCCCTGAGTA CCCTCAGGTT GATGACCTAC TTCTGAGAAG GCTTGTTAAA
AAACCAGAGC GTCAACAGGT TCCTACACGA AAGGATTATG GATCCAAAGT CAGTCTCTTC
TCTCACCTAC CCCAGTACAG CAGACAAAAC TCTCTGACCC AGTTTATGAG CATCCCATCC
TCTGTGATCC ACCCAGCCAT GGTGCGACTC GGCCTGCAGT ACTCCCAGGG CCTGGTCAGT
GGCTCCAATG CCCGGTGTAT TGCCCTGCTT CGTGCCTTGC AGCAGGTGAT TCAGGATTAC
ACAACACCGC CTAATGAAGA ACTCTCCAGG GATCTAGTGA ATAAACTAAA ACCCTACATG
AGCTTCCTGA CTCAGTGCCG TCCCCTGTCA GCGAGCATGC ACAACGCCAT CAAGTTCCTT
AACAAGGAAA TCACCAGTGT GGGCAGTTCC AAGCGGGAAG AGGAGGCCAA GTCAGAACTT
CGAGCAGCCA TTGATCGGTA TGTGCAAGAG AAGATTGTGC TAGCAGCTCA GGCAATTTCA
CGCTTTGCTT ACCAGAAGAT CAGTAATGGA GATGTGATCC TGGTATATGG ATGCTCATCT
CTGGTATCAC GAATTCTTCA GGAGGCTTGG ACAGAGGGCC GGCGGTTTCG GGTGGTAGTG
GTGGACAGCC GGCCATGGCT GGAAGGAAGG CACACACTAC GTTCTCTAGT CCATGCTGGT
GTCCCAGCCT CCTACCTGCT GATTCCTGCA GCCTCCTATG TGCTCCCAGA GGTTTCCAAG
GTGCTATTGG GAGCTCATGC ACTCTTGGCC AACGGGTCTG TGATGTCACG GGTAGGGACA
GCACAGTTAG CCCTGGTGGC TCGAGCCCAT AATGTACCAG TGCTGGTTTG CTGTGAAACA
TACAAGTTCT GTGAGCGTGT GCAGACTGAT GCCTTTGTCT CTAATGAGCT AGATGACCCT
GATGATCTGC AATGTAAGCG GGGAGAACAT GTTGCGCTGG CTAACTGGCA GAACCACGCA
TCCCTACGGT TGTTGAATCT AGTCTATGAT GTGACTCCCC CAGAGCTTGT GGATCTGGTG
ATCACGGAGC TGGGGATGAT CCCTTGCAGT TCTGTACCTG TTGTTCTACG AGTCAAGAGC
AGTGACCAGT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001029288.1
CDS144..1715
Misc Feature(1)147..149(+)
Misc Feature(2)399..401(+)
Misc Feature(3)798..1694(+)
Misc Feature(4)798..1670(+)
Exon (1)1..174
Gene:EIF2B4
Gene Synonym:
Exon (2)175..218
Gene:EIF2B4
Gene Synonym:
Exon (3)219..354
Gene:EIF2B4
Gene Synonym:
Exon (4)355..561
Gene:EIF2B4
Gene Synonym:
Exon (5)562..641
Gene:EIF2B4
Gene Synonym:
Exon (6)642..733
Gene:EIF2B4
Gene Synonym:
Exon (7)734..848
Gene:EIF2B4
Gene Synonym:
Exon (8)849..925
Gene:EIF2B4
Gene Synonym:
Exon (9)926..1028
Gene:EIF2B4
Gene Synonym:
Exon (10)1029..1156
Gene:EIF2B4
Gene Synonym:
Exon (11)1157..1334
Gene:EIF2B4
Gene Synonym:
Exon (12)1335..1515
Gene:EIF2B4
Gene Synonym:
Exon (13)1516..1763
Gene:EIF2B4
Gene Synonym:
Translation
Position Chain Variation Link
11 11 g, t dbSNP:561557212
13 13 a, c dbSNP:540244387
27 27 c, t dbSNP:528039279
28 28 c, g dbSNP:761436350
42 42 a, g dbSNP:547438085
94 94 c, t dbSNP:564213305
100 100 c, g dbSNP:750144681
150 150 g, t dbSNP:182127717
153 153 c, g dbSNP:371924054
158 158 c, t dbSNP:575951063
163 163 c, g, t dbSNP:764607200
167 167 c, t dbSNP:760688449
168 168 c, t dbSNP:368043444
170 170 c, t dbSNP:113310794
171 171 g, t dbSNP:542570644
173 173 a, g dbSNP:759657701
179 179 a, g dbSNP:778090500
180 180 a, g dbSNP:756513459
185 185 c, t dbSNP:753104792
194 194 c, g dbSNP:190259653
204 204 c, t dbSNP:754958652
206 206 c, t dbSNP:751668695
208 208 -, c dbSNP:764709491
208 208 c, t dbSNP:766637381
210 210 c, g dbSNP:769672829
212 212 g, t dbSNP:559997020
217 217 g, t dbSNP:137963149
219 219 c, g dbSNP:747925994
220 220 a, c dbSNP:752068283
223 223 c, t dbSNP:766541364
224 224 a, g dbSNP:758587510
227 227 -, g dbSNP:761106515
227 227 a, g dbSNP:750630633
231 231 c, g dbSNP:780113294
236 236 a, g dbSNP:760328185
243 243 a, g dbSNP:775357878
248 248 a, g dbSNP:767281618
251 251 a, g dbSNP:201350814
252 252 c, g dbSNP:759503915
255 255 c, g dbSNP:144798741
268 268 a, t dbSNP:774312870
272 272 a, g dbSNP:770388291
277 277 a, g dbSNP:748820541
282 282 a, c dbSNP:368905502
284 284 -, gaagaa dbSNP:775790323
287 287 -, gaa dbSNP:772427644
287 287 a, g dbSNP:769443508
299 299 a, g dbSNP:747824895
306 306 a, g dbSNP:756565356
307 307 a, g dbSNP:781612114
308 308 g, t dbSNP:768953969
309 309 a, g dbSNP:747515908
323 323 a, t dbSNP:780733138
325 325 g, t dbSNP:139521128
328 328 c, t dbSNP:750540614
331 331 c, t dbSNP:779208304
332 332 g, t dbSNP:377209171
336 336 g, t dbSNP:754066143
339 339 a, g dbSNP:200290985
346 346 a, g dbSNP:759281615
347 347 a, g dbSNP:751451207
348 348 c, t dbSNP:766318671
359 359 c, t dbSNP:1141530
362 362 a, c dbSNP:750413412
364 364 c, t dbSNP:764777030
365 365 c, t dbSNP:547931123
381 381 a, t dbSNP:776294002
382 382 c, t dbSNP:763729559
383 383 a, g dbSNP:368615356
392 392 a, g, t dbSNP:371589780
398 398 c, t dbSNP:775992226
399 399 a, t dbSNP:530363694
403 403 -, ctc dbSNP:769733183
405 405 c, g, t dbSNP:774760656
406 406 a, g dbSNP:146262118
413 413 a, g dbSNP:770994421
421 421 c, t dbSNP:34155621
422 422 c, t dbSNP:373805172
425 425 a, t dbSNP:777985531
426 426 c, t dbSNP:756288383
434 434 a, g dbSNP:201950471
437 437 c, g dbSNP:779806044
438 438 a, g dbSNP:758233858
445 445 c, g, t dbSNP:368061442
446 446 a, g dbSNP:369882701
454 454 a, g dbSNP:753288781
458 458 a, t dbSNP:763725349
474 474 a, g dbSNP:760388895
475 475 a, c dbSNP:775331954
477 477 a, c, t dbSNP:760002611
478 478 a, g dbSNP:774864445
479 479 g, t dbSNP:113220975
487 487 a, g dbSNP:771349222
489 489 c, g dbSNP:749874646
503 503 c, g dbSNP:773154770
514 514 -, gg dbSNP:748102093
515 515 a, g dbSNP:141872864
522 522 c, g dbSNP:374686395
528 528 a, g dbSNP:146732803
529 529 c, t dbSNP:149520370
535 535 c, t dbSNP:371253140
541 541 -, cagctggag dbSNP:769575268
544 544 c, t dbSNP:778839851
549 549 a, g dbSNP:757134177
553 553 c, g dbSNP:753868838
555 555 c, t dbSNP:763488648
557 557 c, t dbSNP:755723756
570 570 c, t dbSNP:754665816
571 571 a, g, t dbSNP:368243788
572 572 c, t dbSNP:149121402
576 576 c, t dbSNP:750850634
581 581 a, g dbSNP:765743112
584 584 c, t dbSNP:761810644
598 598 a, g dbSNP:569664227
600 600 c, g dbSNP:776685186
610 610 a, g dbSNP:768779136
626 626 a, g dbSNP:147672203
629 629 a, g dbSNP:201503266
633 633 a, c dbSNP:775819795
634 634 a, g dbSNP:770591078
648 648 a, g, t dbSNP:746547024
661 661 a, g dbSNP:779502336
662 662 c, t dbSNP:758061891
675 675 a, t dbSNP:745575196
678 678 c, t dbSNP:565245777
689 689 c, t dbSNP:757657538
709 709 a, g dbSNP:375458330
713 713 c, g, t dbSNP:756090304
715 715 c, t dbSNP:772991139
726 726 a, t dbSNP:752818834
730 730 g, t dbSNP:767553031
735 735 a, t dbSNP:200307171
737 737 c, g dbSNP:766717475
743 743 c, g, t dbSNP:753667257
768 768 c, t dbSNP:113994029
769 769 a, g dbSNP:113994028
771 771 c, g dbSNP:760641987
773 773 c, t dbSNP:775331791
782 782 c, g dbSNP:560111940
784 784 a, c, g dbSNP:773935313
791 791 a, g dbSNP:770574434
797 797 a, g dbSNP:748998115
798 798 c, g dbSNP:778177199
800 800 c, t dbSNP:376346942
817 817 a, g dbSNP:757694836
825 825 a, g dbSNP:748646310
826 826 c, t dbSNP:113994027
827 827 c, g dbSNP:781570273
843 843 c, g dbSNP:755047066
867 867 a, c, g dbSNP:151290164
869 869 a, g dbSNP:770144131
871 871 c, t dbSNP:113994030
872 872 a, g dbSNP:41288829
884 884 a, g dbSNP:769277328
885 885 c, t dbSNP:747580846
899 899 a, g dbSNP:780262752
901 901 c, t dbSNP:758593908
910 910 a, t dbSNP:750621545
915 915 c, t dbSNP:141722828
920 920 c, t dbSNP:755881182
921 921 a, g dbSNP:752440296
934 934 a, c dbSNP:143926434
941 941 c, t dbSNP:764856179
947 947 c, g dbSNP:761353843
948 948 -, ct dbSNP:775042965
949 949 c, g, t dbSNP:113994031
955 955 c, t dbSNP:182331835
956 956 c, g dbSNP:761145823
968 968 c, g, t dbSNP:149782530
972 972 a, g, t dbSNP:774281750
980 980 c, t dbSNP:771082359
981 981 c, t dbSNP:749471127
984 984 a, g dbSNP:777992006
989 989 a, g dbSNP:538217203
1006 1006 a, g dbSNP:754707878
1008 1008 a, c dbSNP:577147537
1011 1011 -, t dbSNP:767850637
1012 1012 c, t dbSNP:190848009
1015 1015 a, g dbSNP:368372393
1017 1017 c, t dbSNP:779887864
1018 1018 a, g dbSNP:758251555
1023 1023 a, g dbSNP:763088766
1044 1044 c, t dbSNP:755715511
1045 1045 a, g dbSNP:781536399
1048 1048 c, t dbSNP:780909643
1050 1050 c, g dbSNP:377339042
1059 1059 c, g, t dbSNP:78599355
1064 1064 c, t dbSNP:758877324
1069 1069 a, g dbSNP:750965977
1073 1073 a, g dbSNP:373520608
1076 1076 a, g dbSNP:761908506
1078 1078 c, t dbSNP:776661452
1081 1081 c, t dbSNP:560532019
1083 1083 c, t dbSNP:760874790
1090 1090 c, g dbSNP:184607650
1092 1092 c, t dbSNP:770898806
1094 1094 a, g dbSNP:749208137
1097 1097 a, g dbSNP:773304003
1098 1098 a, g dbSNP:769371305
1104 1104 c, t dbSNP:200954953
1115 1115 c, t dbSNP:780633076
1123 1123 c, t dbSNP:754642030
1124 1124 c, g dbSNP:746687079
1164 1164 c, g, t dbSNP:145468921
1167 1167 g, t dbSNP:768087185
1174 1174 a, g dbSNP:141025043
1184 1184 a, g dbSNP:144016401
1193 1193 g, t dbSNP:746628836
1195 1195 c, t dbSNP:779707712
1200 1200 a, g dbSNP:778375725
1203 1203 a, c, t dbSNP:779319688
1204 1204 a, g dbSNP:368168999
1211 1211 c, t dbSNP:754354365
1212 1212 c, t dbSNP:113994032
1213 1213 a, g dbSNP:113994033
1215 1215 c, g, t dbSNP:767857138
1224 1224 a, g dbSNP:375697618
1231 1231 a, c, g dbSNP:201216936
1234 1234 a, g dbSNP:113994034
1238 1238 a, g dbSNP:776461313
1245 1245 a, g dbSNP:768160292
1254 1254 c, t dbSNP:760119905
1259 1259 a, g dbSNP:775060569
1263 1263 c, t dbSNP:113994035
1264 1264 a, g dbSNP:371946487
1267 1267 c, t dbSNP:771710835
1272 1272 c, g dbSNP:745579285
1277 1277 c, t dbSNP:779229365
1282 1282 a, g dbSNP:771240893
1286 1286 c, t dbSNP:749762984
1298 1298 c, t dbSNP:144124145
1308 1308 c, g dbSNP:756150677
1315 1315 a, c, g, t dbSNP:113994036
1324 1324 c, t dbSNP:755225054
1339 1339 c, t dbSNP:755135093
1342 1342 a, g dbSNP:139134121
1344 1344 a, g dbSNP:780149885
1347 1347 c, t dbSNP:757012537
1361 1361 c, t dbSNP:753573267
1372 1372 c, g dbSNP:764012305
1373 1373 c, t dbSNP:756024921
1376 1376 c, t dbSNP:1058065
1392 1392 a, c, t dbSNP:759071387
1418 1418 a, g dbSNP:747532557
1420 1420 c, t dbSNP:369616643
1426 1426 a, g dbSNP:377146994
1433 1433 c, t dbSNP:773426946
1436 1436 c, t dbSNP:770318466
1438 1438 c, t dbSNP:748621364
1454 1454 c, t dbSNP:777297913
1464 1464 c, t dbSNP:372970925
1478 1478 a, g dbSNP:747094092
1479 1479 c, t dbSNP:759669141
1480 1480 g, t dbSNP:767391188
1481 1481 c, t dbSNP:758540260
1482 1482 a, g dbSNP:746154598
1493 1493 c, t dbSNP:777400118
1503 1503 g, t dbSNP:755934909
1520 1520 c, t dbSNP:769522881
1526 1526 c, t dbSNP:141403112
1530 1530 a, c dbSNP:768318439
1535 1535 a, g dbSNP:148810263
1536 1536 c, t dbSNP:113994038
1542 1542 c, t dbSNP:138249238
1543 1543 a, g dbSNP:751555693
1546 1546 a, g dbSNP:779387577
1554 1554 g, t dbSNP:757943510
1555 1555 a, t dbSNP:749983780
1559 1559 a, g dbSNP:764983689
1564 1564 c, g dbSNP:761628155
1574 1574 c, g dbSNP:754244635
1581 1581 a, g dbSNP:145784671
1585 1585 c, g dbSNP:761059364
1590 1590 c, t dbSNP:113994039
1591 1591 a, g dbSNP:776097463
1602 1602 c, t dbSNP:1058075
1607 1607 c, t dbSNP:1058076
1608 1608 c, t dbSNP:113994040
1613 1613 c, t dbSNP:376099446
1614 1614 a, g dbSNP:771128051
1620 1620 a, c, t dbSNP:772479767
1621 1621 a, c dbSNP:768526271
1622 1622 a, c dbSNP:746868938
1626 1626 c, g dbSNP:780050233
1632 1632 a, g dbSNP:757777425
1638 1638 a, g dbSNP:1058077
1648 1648 c, t dbSNP:749942048
1649 1649 a, g, t dbSNP:146687759
1676 1676 c, t dbSNP:753589996
1688 1688 c, t dbSNP:764517799
1692 1692 c, t dbSNP:756524864
1694 1694 a, c dbSNP:753088593
1699 1699 a, g dbSNP:768076303
1700 1700 a, g dbSNP:760017546
1702 1702 a, g dbSNP:774390579
1716 1716 c, g, t dbSNP:41288827
1717 1717 a, g dbSNP:773440658
1721 1721 -, g dbSNP:768539625
1722 1722 a, g dbSNP:768275134
1725 1725 -, c dbSNP:746849100
1725 1725 c, t dbSNP:746821092
1729 1729 a, g dbSNP:775212686
1743 1743 c, t dbSNP:772036498
1746 1746 a, g dbSNP:371845186
1749 1749 c, t dbSNP:745852708
1754 1754 c, t dbSNP:367568951
1755 1755 c, t dbSNP:779368638
1758 1758 c, g dbSNP:756863469

Target ORF information:

RefSeq Version NM_001034116
Organism Homo sapiens (human)
Definition Homo sapiens eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001034116

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCTGCTG TGGCCGTGGC TGTTCGCGAG GACTCGGGAT CCGGGATGAA GGCGGAGCTT 
CCCCCTGGGC CTGGGGCAGT GGGGAGGGAA ATGACCAAAG AAGAAAAGCT GCAGCTTCGG
AAGGAAAAGA AACAGCAGAA GAAGAAACGG AAGGAAGAAA AGGGGGCAGA ACCAGAGACT
GGCTCTGCTG TATCTGCAGC CCAATGTCAA GTAGGCCCAA CCAGAGAACT GCCAGAATCG
GGCATTCAGT TGGGCACTCC TCGGGAGAAA GTTCCAGCTG GTCGGAGTAA GGCCGAACTT
CGGGCTGAGC GTCGAGCCAA GCAGGAGGCC GAGCGGGCCC TGAAACAGGC AAGAAAAGGG
GAACAAGGAG GACCACCTCC TAAGGCCAGC CCCAGCACAG CTGGAGAAAC CCCCTCAGGA
GTGAAGCGTC TCCCTGAGTA CCCTCAGGTT GATGACCTAC TTCTGAGAAG GCTTGTTAAA
AAACCAGAGC GTCAACAGGT TCCTACACGA AAGGATTATG GATCCAAAGT CAGTCTCTTC
TCTCACCTAC CCCAGTACAG CAGACAAAAC TCTCTGACCC AGTTTATGAG CATCCCATCC
TCTGTGATCC ACCCAGCCAT GGTGCGACTC GGCCTGCAGT ACTCCCAGGG CCTGGTCAGT
GGCTCCAATG CCCGGTGTAT TGCCCTGCTT CGTGCCTTGC AGCAGGTGAT TCAGGATTAC
ACAACACCGC CTAATGAAGA ACTCTCCAGG GATCTAGTGA ATAAACTAAA ACCCTACATG
AGCTTCCTGA CTCAGTGCCG TCCCCTGTCA GCGAGCATGC ACAACGCCAT CAAGTTCCTT
AACAAGGAAA TCACCAGTGT GGGCAGTTCC AAGCGGGAAG AGGAGGCCAA GTCAGAACTT
CGAGCAGCCA TTGATCGGTA TGTGCAAGAG AAGATTGTGC TAGCAGCTCA GGCAATTTCA
CGCTTTGCTT ACCAGAAGAT CAGTAATGGA GATGTGATCC TGGTATATGG ATGCTCATCT
CTGGTATCAC GAATTCTTCA GGAGGCTTGG ACAGAGGGCC GGCGGTTTCG GGTGGTAGTG
GTGGACAGCC GGCCATGGCT GGAAGGAAGG CACACACTAC GTTCTCTAGT CCATGCTGGT
GTCCCAGCCT CCTACCTGCT GATTCCTGCA GCCTCCTATG TGCTCCCAGA GGTTTCCAAG
GTGCTATTGG GAGCTCATGC ACTCTTGGCC AACGGGTCTG TGATGTCACG GGTAGGGACA
GCACAGTTAG CCCTGGTGGC TCGAGCCCAT AATGTACCAG TGCTGGTTTG CTGTGAAACA
TACAAGTTCT GTGAGCGTGT GCAGACTGAT GCCTTTGTCT CTAATGAGCT AGATGACCCT
GATGATCTGC AATGTAAGCG GGGAGAACAT GTTGCGCTGG CTAACTGGCA GAACCACGCA
TCCCTACGGT TGTTGAATCT AGTCTATGAT GTGACTCCCC CAGAGCTTGT GGATCTGGTG
ATCACGGAGC TGGGGATGAT CCCTTGCAGT TCTGTACCTG TTGTTCTACG AGTCAAGAGC
AGTGACCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27281
Accession Version NM_172195.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1632bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product translation initiation factor eIF-2B subunit delta isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AJ011306.1, BC091502.1 and AF112207.1. On May 7, 2008 this sequence version replaced gi:78000152. Summary: Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) is the shortest transcript but encodes the longest isoform (1), also known as the long isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ011306.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCAACCC AGCAGCCGGC TGCGCCGAGT ACTCGTGCCC CCAAACCCTC CCGGAGTCTC 
TCTGGCTCAC TTTGTGCCCT GTTTTCTGAT GCAGACTCGG GATCCGGGAT GAAGGCGGAG
CTTCCCCCTG GGCCTGGGGC AGTGGGGAGG GAAATGACCA AAGAAGAAAA GCTGCAGCTT
CGGAAGGAAA AGAAACAGCA GAAGAAGAAA CGGAAGGAAG AAAAGGGGGC AGAACCAGAG
ACTGGCTCTG CTGTATCTGC AGCCCAATGT CAAGGCCCAA CCAGAGAACT GCCAGAATCG
GGCATTCAGT TGGGCACTCC TCGGGAGAAA GTTCCAGCTG GTCGGAGTAA GGCCGAACTT
CGGGCTGAGC GTCGAGCCAA GCAGGAGGCC GAGCGGGCCC TGAAACAGGC AAGAAAAGGG
GAACAAGGAG GACCACCTCC TAAGGCCAGC CCCAGCACAG CTGGAGAAAC CCCCTCAGGA
GTGAAGCGTC TCCCTGAGTA CCCTCAGGTT GATGACCTAC TTCTGAGAAG GCTTGTTAAA
AAACCAGAGC GTCAACAGGT TCCTACACGA AAGGATTATG GATCCAAAGT CAGTCTCTTC
TCTCACCTAC CCCAGTACAG CAGACAAAAC TCTCTGACCC AGTTTATGAG CATCCCATCC
TCTGTGATCC ACCCAGCCAT GGTGCGACTC GGCCTGCAGT ACTCCCAGGG CCTGGTCAGT
GGCTCCAATG CCCGGTGTAT TGCCCTGCTT CGTGCCTTGC AGCAGGTGAT TCAGGATTAC
ACAACACCGC CTAATGAAGA ACTCTCCAGG GATCTAGTGA ATAAACTAAA ACCCTACATG
AGCTTCCTGA CTCAGTGCCG TCCCCTGTCA GCGAGCATGC ACAACGCCAT CAAGTTCCTT
AACAAGGAAA TCACCAGTGT GGGCAGTTCC AAGCGGGAAG AGGAGGCCAA GTCAGAACTT
CGAGCAGCCA TTGATCGGTA TGTGCAAGAG AAGATTGTGC TAGCAGCTCA GGCAATTTCA
CGCTTTGCTT ACCAGAAGAT CAGTAATGGA GATGTGATCC TGGTATATGG ATGCTCATCT
CTGGTATCAC GAATTCTTCA GGAGGCTTGG ACAGAGGGCC GGCGGTTTCG GGTGGTAGTG
GTGGACAGCC GGCCATGGCT GGAAGGAAGG CACACACTAC GTTCTCTAGT CCATGCTGGT
GTCCCAGCCT CCTACCTGCT GATTCCTGCA GCCTCCTATG TGCTCCCAGA GGTTTCCAAG
GTGCTATTGG GAGCTCATGC ACTCTTGGCC AACGGGTCTG TGATGTCACG GGTAGGGACA
GCACAGTTAG CCCTGGTGGC TCGAGCCCAT AATGTACCAG TGCTGGTTTG CTGTGAAACA
TACAAGTTCT GTGAGCGTGT GCAGACTGAT GCCTTTGTCT CTAATGAGCT AGATGACCCT
GATGATCTGC AATGTAAGCG GGGAGAACAT GTTGCGCTGG CTAACTGGCA GAACCACGCA
TCCCTACGGT TGTTGAATCT AGTCTATGAT GTGACTCCCC CAGAGCTTGT GGATCTGGTG
ATCACGGAGC TGGGGATGAT CCCTTGCAGT TCTGTACCTG TTGTTCTACG AGTCAAGAGC
AGTGACCAGT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_751945.2
CDS40..1671
Misc Feature(1)754..1650(+)
Misc Feature(2)754..1626(+)
Exon (1)1..177
Gene:EIF2B4
Gene Synonym:
Exon (2)178..313
Gene:EIF2B4
Gene Synonym:
Exon (3)314..517
Gene:EIF2B4
Gene Synonym:
Exon (4)518..597
Gene:EIF2B4
Gene Synonym:
Exon (5)598..689
Gene:EIF2B4
Gene Synonym:
Exon (6)690..804
Gene:EIF2B4
Gene Synonym:
Exon (7)805..881
Gene:EIF2B4
Gene Synonym:
Exon (8)882..984
Gene:EIF2B4
Gene Synonym:
Exon (9)985..1112
Gene:EIF2B4
Gene Synonym:
Exon (10)1113..1290
Gene:EIF2B4
Gene Synonym:
Exon (11)1291..1471
Gene:EIF2B4
Gene Synonym:
Exon (12)1472..1719
Gene:EIF2B4
Gene Synonym:
Translation
Position Chain Variation Link
2 2 c, t dbSNP:45561836
3 3 c, t dbSNP:371157163
11 11 g, t dbSNP:368555120
21 21 c, t dbSNP:764517220
23 23 c, t dbSNP:761158565
24 24 c, t dbSNP:753297583
28 28 a, g dbSNP:45479901
36 36 c, g dbSNP:767463519
39 39 a, c, g dbSNP:542460570
41 41 c, t dbSNP:774390106
62 62 c, t dbSNP:771202581
63 63 g, t dbSNP:375648525
71 71 c, t dbSNP:776566253
74 74 c, g dbSNP:768627287
75 75 c, t dbSNP:746918564
87 87 a, c dbSNP:780076284
91 91 c, t dbSNP:771439416
98 98 -, t dbSNP:762306812
107 107 c, t dbSNP:371092853
112 112 c, g, t dbSNP:756905597
127 127 c, g dbSNP:756551239
138 138 a, g dbSNP:778090500
139 139 a, g dbSNP:756513459
144 144 c, t dbSNP:753104792
153 153 c, g dbSNP:190259653
163 163 c, t dbSNP:754958652
165 165 c, t dbSNP:751668695
167 167 -, c dbSNP:764709491
167 167 c, t dbSNP:766637381
169 169 c, g dbSNP:769672829
171 171 g, t dbSNP:559997020
176 176 g, t dbSNP:137963149
178 178 c, g dbSNP:747925994
179 179 a, c dbSNP:752068283
182 182 c, t dbSNP:766541364
183 183 a, g dbSNP:758587510
186 186 -, g dbSNP:761106515
186 186 a, g dbSNP:750630633
190 190 c, g dbSNP:780113294
195 195 a, g dbSNP:760328185
202 202 a, g dbSNP:775357878
207 207 a, g dbSNP:767281618
210 210 a, g dbSNP:201350814
211 211 c, g dbSNP:759503915
214 214 c, g dbSNP:144798741
227 227 a, t dbSNP:774312870
231 231 a, g dbSNP:770388291
236 236 a, g dbSNP:748820541
241 241 a, c dbSNP:368905502
243 243 -, gaagaa dbSNP:775790323
246 246 -, gaa dbSNP:772427644
246 246 a, g dbSNP:769443508
258 258 a, g dbSNP:747824895
265 265 a, g dbSNP:756565356
266 266 a, g dbSNP:781612114
267 267 g, t dbSNP:768953969
268 268 a, g dbSNP:747515908
282 282 a, t dbSNP:780733138
284 284 g, t dbSNP:139521128
287 287 c, t dbSNP:750540614
290 290 c, t dbSNP:779208304
291 291 g, t dbSNP:377209171
295 295 g, t dbSNP:754066143
298 298 a, g dbSNP:200290985
305 305 a, g dbSNP:759281615
306 306 a, g dbSNP:751451207
307 307 c, t dbSNP:766318671
315 315 c, t dbSNP:1141530
318 318 a, c dbSNP:750413412
320 320 c, t dbSNP:764777030
321 321 c, t dbSNP:547931123
337 337 a, t dbSNP:776294002
338 338 c, t dbSNP:763729559
339 339 a, g dbSNP:368615356
348 348 a, g, t dbSNP:371589780
354 354 c, t dbSNP:775992226
355 355 a, t dbSNP:530363694
359 359 -, ctc dbSNP:769733183
361 361 c, g, t dbSNP:774760656
362 362 a, g dbSNP:146262118
369 369 a, g dbSNP:770994421
377 377 c, t dbSNP:34155621
378 378 c, t dbSNP:373805172
381 381 a, t dbSNP:777985531
382 382 c, t dbSNP:756288383
390 390 a, g dbSNP:201950471
393 393 c, g dbSNP:779806044
394 394 a, g dbSNP:758233858
401 401 c, g, t dbSNP:368061442
402 402 a, g dbSNP:369882701
410 410 a, g dbSNP:753288781
414 414 a, t dbSNP:763725349
430 430 a, g dbSNP:760388895
431 431 a, c dbSNP:775331954
433 433 a, c, t dbSNP:760002611
434 434 a, g dbSNP:774864445
435 435 g, t dbSNP:113220975
443 443 a, g dbSNP:771349222
445 445 c, g dbSNP:749874646
459 459 c, g dbSNP:773154770
470 470 -, gg dbSNP:748102093
471 471 a, g dbSNP:141872864
478 478 c, g dbSNP:374686395
484 484 a, g dbSNP:146732803
485 485 c, t dbSNP:149520370
491 491 c, t dbSNP:371253140
497 497 -, cagctggag dbSNP:769575268
500 500 c, t dbSNP:778839851
505 505 a, g dbSNP:757134177
509 509 c, g dbSNP:753868838
511 511 c, t dbSNP:763488648
513 513 c, t dbSNP:755723756
526 526 c, t dbSNP:754665816
527 527 a, g, t dbSNP:368243788
528 528 c, t dbSNP:149121402
532 532 c, t dbSNP:750850634
537 537 a, g dbSNP:765743112
540 540 c, t dbSNP:761810644
554 554 a, g dbSNP:569664227
556 556 c, g dbSNP:776685186
566 566 a, g dbSNP:768779136
582 582 a, g dbSNP:147672203
585 585 a, g dbSNP:201503266
589 589 a, c dbSNP:775819795
590 590 a, g dbSNP:770591078
604 604 a, g, t dbSNP:746547024
617 617 a, g dbSNP:779502336
618 618 c, t dbSNP:758061891
631 631 a, t dbSNP:745575196
634 634 c, t dbSNP:565245777
645 645 c, t dbSNP:757657538
665 665 a, g dbSNP:375458330
669 669 c, g, t dbSNP:756090304
671 671 c, t dbSNP:772991139
682 682 a, t dbSNP:752818834
686 686 g, t dbSNP:767553031
691 691 a, t dbSNP:200307171
693 693 c, g dbSNP:766717475
699 699 c, g, t dbSNP:753667257
724 724 c, t dbSNP:113994029
725 725 a, g dbSNP:113994028
727 727 c, g dbSNP:760641987
729 729 c, t dbSNP:775331791
738 738 c, g dbSNP:560111940
740 740 a, c, g dbSNP:773935313
747 747 a, g dbSNP:770574434
753 753 a, g dbSNP:748998115
754 754 c, g dbSNP:778177199
756 756 c, t dbSNP:376346942
773 773 a, g dbSNP:757694836
781 781 a, g dbSNP:748646310
782 782 c, t dbSNP:113994027
783 783 c, g dbSNP:781570273
799 799 c, g dbSNP:755047066
823 823 a, c, g dbSNP:151290164
825 825 a, g dbSNP:770144131
827 827 c, t dbSNP:113994030
828 828 a, g dbSNP:41288829
840 840 a, g dbSNP:769277328
841 841 c, t dbSNP:747580846
855 855 a, g dbSNP:780262752
857 857 c, t dbSNP:758593908
866 866 a, t dbSNP:750621545
871 871 c, t dbSNP:141722828
876 876 c, t dbSNP:755881182
877 877 a, g dbSNP:752440296
890 890 a, c dbSNP:143926434
897 897 c, t dbSNP:764856179
903 903 c, g dbSNP:761353843
904 904 -, ct dbSNP:775042965
905 905 c, g, t dbSNP:113994031
911 911 c, t dbSNP:182331835
912 912 c, g dbSNP:761145823
924 924 c, g, t dbSNP:149782530
928 928 a, g, t dbSNP:774281750
936 936 c, t dbSNP:771082359
937 937 c, t dbSNP:749471127
940 940 a, g dbSNP:777992006
945 945 a, g dbSNP:538217203
962 962 a, g dbSNP:754707878
964 964 a, c dbSNP:577147537
967 967 -, t dbSNP:767850637
968 968 c, t dbSNP:190848009
971 971 a, g dbSNP:368372393
973 973 c, t dbSNP:779887864
974 974 a, g dbSNP:758251555
979 979 a, g dbSNP:763088766
1000 1000 c, t dbSNP:755715511
1001 1001 a, g dbSNP:781536399
1004 1004 c, t dbSNP:780909643
1006 1006 c, g dbSNP:377339042
1015 1015 c, g, t dbSNP:78599355
1020 1020 c, t dbSNP:758877324
1025 1025 a, g dbSNP:750965977
1029 1029 a, g dbSNP:373520608
1032 1032 a, g dbSNP:761908506
1034 1034 c, t dbSNP:776661452
1037 1037 c, t dbSNP:560532019
1039 1039 c, t dbSNP:760874790
1046 1046 c, g dbSNP:184607650
1048 1048 c, t dbSNP:770898806
1050 1050 a, g dbSNP:749208137
1053 1053 a, g dbSNP:773304003
1054 1054 a, g dbSNP:769371305
1060 1060 c, t dbSNP:200954953
1071 1071 c, t dbSNP:780633076
1079 1079 c, t dbSNP:754642030
1080 1080 c, g dbSNP:746687079
1120 1120 c, g, t dbSNP:145468921
1123 1123 g, t dbSNP:768087185
1130 1130 a, g dbSNP:141025043
1140 1140 a, g dbSNP:144016401
1149 1149 g, t dbSNP:746628836
1151 1151 c, t dbSNP:779707712
1156 1156 a, g dbSNP:778375725
1159 1159 a, c, t dbSNP:779319688
1160 1160 a, g dbSNP:368168999
1167 1167 c, t dbSNP:754354365
1168 1168 c, t dbSNP:113994032
1169 1169 a, g dbSNP:113994033
1171 1171 c, g, t dbSNP:767857138
1180 1180 a, g dbSNP:375697618
1187 1187 a, c, g dbSNP:201216936
1190 1190 a, g dbSNP:113994034
1194 1194 a, g dbSNP:776461313
1201 1201 a, g dbSNP:768160292
1210 1210 c, t dbSNP:760119905
1215 1215 a, g dbSNP:775060569
1219 1219 c, t dbSNP:113994035
1220 1220 a, g dbSNP:371946487
1223 1223 c, t dbSNP:771710835
1228 1228 c, g dbSNP:745579285
1233 1233 c, t dbSNP:779229365
1238 1238 a, g dbSNP:771240893
1242 1242 c, t dbSNP:749762984
1254 1254 c, t dbSNP:144124145
1264 1264 c, g dbSNP:756150677
1271 1271 a, c, g, t dbSNP:113994036
1280 1280 c, t dbSNP:755225054
1295 1295 c, t dbSNP:755135093
1298 1298 a, g dbSNP:139134121
1300 1300 a, g dbSNP:780149885
1303 1303 c, t dbSNP:757012537
1317 1317 c, t dbSNP:753573267
1328 1328 c, g dbSNP:764012305
1329 1329 c, t dbSNP:756024921
1332 1332 c, t dbSNP:1058065
1348 1348 a, c, t dbSNP:759071387
1374 1374 a, g dbSNP:747532557
1376 1376 c, t dbSNP:369616643
1382 1382 a, g dbSNP:377146994
1389 1389 c, t dbSNP:773426946
1392 1392 c, t dbSNP:770318466
1394 1394 c, t dbSNP:748621364
1410 1410 c, t dbSNP:777297913
1420 1420 c, t dbSNP:372970925
1434 1434 a, g dbSNP:747094092
1435 1435 c, t dbSNP:759669141
1436 1436 g, t dbSNP:767391188
1437 1437 c, t dbSNP:758540260
1438 1438 a, g dbSNP:746154598
1449 1449 c, t dbSNP:777400118
1459 1459 g, t dbSNP:755934909
1476 1476 c, t dbSNP:769522881
1482 1482 c, t dbSNP:141403112
1486 1486 a, c dbSNP:768318439
1491 1491 a, g dbSNP:148810263
1492 1492 c, t dbSNP:113994038
1498 1498 c, t dbSNP:138249238
1499 1499 a, g dbSNP:751555693
1502 1502 a, g dbSNP:779387577
1510 1510 g, t dbSNP:757943510
1511 1511 a, t dbSNP:749983780
1515 1515 a, g dbSNP:764983689
1520 1520 c, g dbSNP:761628155
1530 1530 c, g dbSNP:754244635
1537 1537 a, g dbSNP:145784671
1541 1541 c, g dbSNP:761059364
1546 1546 c, t dbSNP:113994039
1547 1547 a, g dbSNP:776097463
1558 1558 c, t dbSNP:1058075
1563 1563 c, t dbSNP:1058076
1564 1564 c, t dbSNP:113994040
1569 1569 c, t dbSNP:376099446
1570 1570 a, g dbSNP:771128051
1576 1576 a, c, t dbSNP:772479767
1577 1577 a, c dbSNP:768526271
1578 1578 a, c dbSNP:746868938
1582 1582 c, g dbSNP:780050233
1588 1588 a, g dbSNP:757777425
1594 1594 a, g dbSNP:1058077
1604 1604 c, t dbSNP:749942048
1605 1605 a, g, t dbSNP:146687759
1632 1632 c, t dbSNP:753589996
1644 1644 c, t dbSNP:764517799
1648 1648 c, t dbSNP:756524864
1650 1650 a, c dbSNP:753088593
1655 1655 a, g dbSNP:768076303
1656 1656 a, g dbSNP:760017546
1658 1658 a, g dbSNP:774390579
1672 1672 c, g, t dbSNP:41288827
1673 1673 a, g dbSNP:773440658
1677 1677 -, g dbSNP:768539625
1678 1678 a, g dbSNP:768275134
1681 1681 -, c dbSNP:746849100
1681 1681 c, t dbSNP:746821092
1685 1685 a, g dbSNP:775212686
1699 1699 c, t dbSNP:772036498
1702 1702 a, g dbSNP:371845186
1705 1705 c, t dbSNP:745852708
1710 1710 c, t dbSNP:367568951
1711 1711 c, t dbSNP:779368638
1714 1714 c, g dbSNP:756863469

Target ORF information:

RefSeq Version NM_172195
Organism Homo sapiens (human)
Definition Homo sapiens eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_172195

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCAACCC AGCAGCCGGC TGCGCCGAGT ACTCGTGCCC CCAAACCCTC CCGGAGTCTC 
TCTGGCTCAC TTTGTGCCCT GTTTTCTGAT GCAGACTCGG GATCCGGGAT GAAGGCGGAG
CTTCCCCCTG GGCCTGGGGC AGTGGGGAGG GAAATGACCA AAGAAGAAAA GCTGCAGCTT
CGGAAGGAAA AGAAACAGCA GAAGAAGAAA CGGAAGGAAG AAAAGGGGGC AGAACCAGAG
ACTGGCTCTG CTGTATCTGC AGCCCAATGT CAAGGCCCAA CCAGAGAACT GCCAGAATCG
GGCATTCAGT TGGGCACTCC TCGGGAGAAA GTTCCAGCTG GTCGGAGTAA GGCCGAACTT
CGGGCTGAGC GTCGAGCCAA GCAGGAGGCC GAGCGGGCCC TGAAACAGGC AAGAAAAGGG
GAACAAGGAG GACCACCTCC TAAGGCCAGC CCCAGCACAG CTGGAGAAAC CCCCTCAGGA
GTGAAGCGTC TCCCTGAGTA CCCTCAGGTT GATGACCTAC TTCTGAGAAG GCTTGTTAAA
AAACCAGAGC GTCAACAGGT TCCTACACGA AAGGATTATG GATCCAAAGT CAGTCTCTTC
TCTCACCTAC CCCAGTACAG CAGACAAAAC TCTCTGACCC AGTTTATGAG CATCCCATCC
TCTGTGATCC ACCCAGCCAT GGTGCGACTC GGCCTGCAGT ACTCCCAGGG CCTGGTCAGT
GGCTCCAATG CCCGGTGTAT TGCCCTGCTT CGTGCCTTGC AGCAGGTGAT TCAGGATTAC
ACAACACCGC CTAATGAAGA ACTCTCCAGG GATCTAGTGA ATAAACTAAA ACCCTACATG
AGCTTCCTGA CTCAGTGCCG TCCCCTGTCA GCGAGCATGC ACAACGCCAT CAAGTTCCTT
AACAAGGAAA TCACCAGTGT GGGCAGTTCC AAGCGGGAAG AGGAGGCCAA GTCAGAACTT
CGAGCAGCCA TTGATCGGTA TGTGCAAGAG AAGATTGTGC TAGCAGCTCA GGCAATTTCA
CGCTTTGCTT ACCAGAAGAT CAGTAATGGA GATGTGATCC TGGTATATGG ATGCTCATCT
CTGGTATCAC GAATTCTTCA GGAGGCTTGG ACAGAGGGCC GGCGGTTTCG GGTGGTAGTG
GTGGACAGCC GGCCATGGCT GGAAGGAAGG CACACACTAC GTTCTCTAGT CCATGCTGGT
GTCCCAGCCT CCTACCTGCT GATTCCTGCA GCCTCCTATG TGCTCCCAGA GGTTTCCAAG
GTGCTATTGG GAGCTCATGC ACTCTTGGCC AACGGGTCTG TGATGTCACG GGTAGGGACA
GCACAGTTAG CCCTGGTGGC TCGAGCCCAT AATGTACCAG TGCTGGTTTG CTGTGAAACA
TACAAGTTCT GTGAGCGTGT GCAGACTGAT GCCTTTGTCT CTAATGAGCT AGATGACCCT
GATGATCTGC AATGTAAGCG GGGAGAACAT GTTGCGCTGG CTAACTGGCA GAACCACGCA
TCCCTACGGT TGTTGAATCT AGTCTATGAT GTGACTCCCC CAGAGCTTGT GGATCTGGTG
ATCACGGAGC TGGGGATGAT CCCTTGCAGT TCTGTACCTG TTGTTCTACG AGTCAAGAGC
AGTGACCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.