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EIF2B2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol EIF2B2
Entrez Gene ID 8892
Full Name eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
Synonyms EIF-2Bbeta, EIF2B
General protein information
Preferred Names
translation initiation factor eIF-2B subunit beta
Names
translation initiation factor eIF-2B subunit beta
S20I15
S20III15
eIF-2B GDP-GTP exchange factor subunit beta
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

14

14q24.3

Summary This gene encodes the beta subunit of eukaryotic initiation factor-2B (EIF2B). EIF2B is involved in protein synthesis and exchanges GDP and GTP for its activation and deactivation. [provided by RefSeq, Aug 2011]. lac of sum
Disorder MIM:

606454

Disorder Html: Leukoencephalopathy with vanishing white matter, 603896 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_014239 NP_055054 translation initiation factor eIF-2B subunit beta



Homo sapiens (human) EIF2B2 NP_055054.1
Pan troglodytes (chimpanzee) EIF2B2 XP_001159118.1
Macaca mulatta (Rhesus monkey) EIF2B2 XP_001096586.1
Canis lupus familiaris (dog) EIF2B2 XP_547911.3
Bos taurus (cattle) EIF2B2 NP_001015593.1
Mus musculus (house mouse) Eif2b2 NP_663420.1
Rattus norvegicus (Norway rat) Eif2b2 NP_114447.2
Gallus gallus (chicken) EIF2B2 NP_001006481.1
Danio rerio (zebrafish) eif2b2 NP_998068.1
Drosophila melanogaster (fruit fly) eIF2B-beta NP_570020.1
Arabidopsis thaliana (thale cress) AT3G07300 NP_001030655.1
Xenopus (Silurana) tropicalis (western clawed frog) eif2b2 NP_001164986.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EIF2B2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2B2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22319
NM_014239 Homo sapiens eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa (EIF2B2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22319
Clone ID Related Accession (Same CDS sequence) NM_014239
Accession Version NM_014239.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1056bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product translation initiation factor eIF-2B subunit beta
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AL049780.4, BC011750.2 and AI265790.1. This sequence is a reference standard in the RefSeqGene project. On Oct 10, 2009 this sequence version replaced gi:77404352. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000494.2, AF035280.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCCGGGAT CCGCAGCGAA GGGCTCGGAG TTGTCAGAGA GGATCGAGAG CTTCGTGGAG 
ACCCTGAAGC GGGGTGGTGG GCCGCGCAGC TCCGAGGAAA TGGCTCGGGA GACCCTAGGG
TTGCTGCGCC AGATCATCAC GGACCACCGC TGGAGCAACG CGGGGGAGCT GATGGAGCTG
ATCCGCAGAG AGGGCAGGAG GATGACGGCC GCTCAGCCCT CCGAGACCAC CGTGGGCAAC
ATGGTGCGGA GAGTGCTCAA GATTATCCGG GAGGAGTATG GCAGACTCCA TGGACGCAGC
GACGAGAGTG ATCAGCAGGA GTCCCTGCAC AAACTGTTGA CATCCGGAGG CCTAAACGAG
GATTTCAGCT TCCATTATGC CCAACTCCAG TCCAACATCA TTGAGGCGAT TAATGAGCTG
CTAGTGGAGC TGGAAGGGAC AATGGAGAAC ATTGCAGCCC AGGCTCTGGA GCACATTCAC
TCCAATGAGG TGATCATGAC CATTGGCTTC TCCCGAACAG TAGAGGCCTT CCTCAAAGAG
GCTGCCCGAA AGAGGAAATT CCATGTCATT GTAGCAGAGT GTGCTCCTTT CTGCCAGGGT
CATGAAATGG CTGTGAATTT GTCCAAAGCA GGTATTGAGA CAACTGTCAT GACTGATGCT
GCCATTTTTG CCGTTATGTC AAGAGTCAAC AAGGTGATCA TTGGCACGAA GACCATCCTG
GCCAATGGGG CCCTGAGAGC TGTGACAGGA ACTCACACTC TGGCACTGGC AGCAAAACAC
CATTCCACCC CACTCATCGT CTGTGCACCT ATGTTCAAAC TTTCTCCACA GTTCCCCAAT
GAAGAAGACT CATTTCATAA GTTTGTGGCT CCTGAAGAAG TCCTGCCATT CACAGAAGGG
GACATTCTGG AGAAGGTCAG CGTGCATTGC CCTGTGTTTG ACTACGTTCC CCCAGAGCTC
ATTACCCTCT TTATCTCCAA CATTGGTGGG AATGCACCTT CCTACATCTA CCGCCTGATG
AGTGAACTCT ACCATCCTGA TGATCATGTT TTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_055054.1
CDS83..1138
Misc Feature(1)56..58(+)
Misc Feature(2)167..1114(+)
Misc Feature(3)167..1081(+)
Exon (1)1..245
Gene:EIF2B2
Gene Synonym:
Exon (2)246..366
Gene:EIF2B2
Gene Synonym:
Exon (3)367..515
Gene:EIF2B2
Gene Synonym:
Exon (4)516..679
Gene:EIF2B2
Gene Synonym:
Exon (5)680..775
Gene:EIF2B2
Gene Synonym:
Exon (6)776..913
Gene:EIF2B2
Gene Synonym:
Exon (7)914..980
Gene:EIF2B2
Gene Synonym:
Exon (8)981..1541
Gene:EIF2B2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_014239
Organism Homo sapiens (human)
Definition Homo sapiens eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa (EIF2B2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014239

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCCGGGAT CCGCAGCGAA GGGCTCGGAG TTGTCAGAGA GGATCGAGAG CTTCGTGGAG 
ACCCTGAAGC GGGGTGGTGG GCCGCGCAGC TCCGAGGAAA TGGCTCGGGA GACCCTAGGG
TTGCTGCGCC AGATCATCAC GGACCACCGC TGGAGCAACG CGGGGGAGCT GATGGAGCTG
ATCCGCAGAG AGGGCAGGAG GATGACGGCC GCTCAGCCCT CCGAGACCAC CGTGGGCAAC
ATGGTGCGGA GAGTGCTCAA GATTATCCGG GAGGAGTATG GCAGACTCCA TGGACGCAGC
GACGAGAGTG ATCAGCAGGA GTCCCTGCAC AAACTGTTGA CATCCGGAGG CCTAAACGAG
GATTTCAGCT TCCATTATGC CCAACTCCAG TCCAACATCA TTGAGGCGAT TAATGAGCTG
CTAGTGGAGC TGGAAGGGAC AATGGAGAAC ATTGCAGCCC AGGCTCTGGA GCACATTCAC
TCCAATGAGG TGATCATGAC CATTGGCTTC TCCCGAACAG TAGAGGCCTT CCTCAAAGAG
GCTGCCCGAA AGAGGAAATT CCATGTCATT GTAGCAGAGT GTGCTCCTTT CTGCCAGGGT
CATGAAATGG CTGTGAATTT GTCCAAAGCA GGTATTGAGA CAACTGTCAT GACTGATGCT
GCCATTTTTG CCGTTATGTC AAGAGTCAAC AAGGTGATCA TTGGCACGAA GACCATCCTG
GCCAATGGGG CCCTGAGAGC TGTGACAGGA ACTCACACTC TGGCACTGGC AGCAAAACAC
CATTCCACCC CACTCATCGT CTGTGCACCT ATGTTCAAAC TTTCTCCACA GTTCCCCAAT
GAAGAAGACT CATTTCATAA GTTTGTGGCT CCTGAAGAAG TCCTGCCATT CACAGAAGGG
GACATTCTGG AGAAGGTCAG CGTGCATTGC CCTGTGTTTG ACTACGTTCC CCCAGAGCTC
ATTACCCTCT TTATCTCCAA CATTGGTGGG AATGCACCTT CCTACATCTA CCGCCTGATG
AGTGAACTCT ACCATCCTGA TGATCATGTT TTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Vanishing white matter disease: an Italian case with A638G mutation in exon 5 of EIF2B2 gene, an unusual early onset and a long course
Neurol. Sci. 34 (7), 1235-1238 (2013)
Sambati,L., Agati,R., Bacci,A., Bianchi,S. and Capellari,S.


book

Vanishing white matter disease caused by EIF2B2 mutation with the presentation of an adrenoleukodystrophy phenotype
Gene 496 (2), 141-143 (2012)
Alsalem A, Shaheen R and Alkuraya FS.


book

The N-terminal domain of the human eIF2beta subunit and the CK2 phosphorylation sites are required for its function
Biochem. J. 394 (PT 1), 227-236 (2006)
Llorens F, Duarri A, Sarro E, Roher N, Plana M and Itarte E.


book

Identification of ten novel mutations in patients with eIF2B-related disorders
Hum. Mutat. 25 (4), 411 (2005)
Ohlenbusch A, Henneke M, Brockmann K, Goerg M, Hanefeld F, Kohlschutter A and Gartner J.


book

Screening for known mutations in EIF2B genes in a large panel of patients with premature ovarian failure
BMC Womens Health 4 (1), 8 (2004)
Fogli,A., Gauthier-Barichard,F., Schiffmann,R., Vanderhoof,V.H., Bakalov,V.K., Nelson,L.M. and Boespflug-Tanguy,O.


book

Identification of domains and residues within the epsilon subunit of eukaryotic translation initiation factor 2B (eIF2Bepsilon) required for guanine nucleotide exchange reveals a novel activation function promoted by eIF2B complex formation
Mol. Cell. Biol. 20 (11), 3965-3976 (2000)
Gomez E and Pavitt GD.


book

Identification of interprotein interactions between the subunits of eukaryotic initiation factors eIF2 and eIF2B
J. Biol. Chem. 273 (5), 3039-3044 (1998)
Kimball SR, Heinzinger NK, Horetsky RL and Jefferson LS.


book

Identification of a regulatory subcomplex in the guanine nucleotide exchange factor eIF2B that mediates inhibition by phosphorylated eIF2
Mol. Cell. Biol. 16 (11), 6603-6616 (1996)
Yang W and Hinnebusch AG.


book

T-cell activation leads to rapid stimulation of translation initiation factor eIF2B and inactivation of glycogen synthase kinase-3
J. Biol. Chem. 271 (19), 11410-11413 (1996)
Welsh GI, Miyamoto S, Price NT, Safer B and Proud CG.


book

Childhood Ataxia with Central Nervous System Hypomyelination/Vanishing White Matter
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Schiffmann,R., Fogli,A., Van der Knaap,M.S. and Boespflug-Tanguy,O.


 
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