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SGCE cDNA ORF clone, Homo sapiens (human)

Gene Symbol SGCE
Entrez Gene ID 8910
Full Name sarcoglycan, epsilon
Synonyms DYT11, ESG
General protein information
Preferred Names
epsilon-sarcoglycan
Names
epsilon-sarcoglycan
epsilon-SG
dystonia 11, myoclonic
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q21.3

Summary This gene encodes the epsilon member of the sarcoglycan family. Sarcoglycans are transmembrane proteins that are components of the dystrophin-glycoprotein complex, which link the actin cytoskeleton to the extracellular matrix. Unlike other family members which are predominantly expressed in striated muscle, the epsilon sarcoglycan is more broadly expressed. Mutations in this gene are associated with myoclonus-dystonia syndrome. This gene is imprinted, with preferential expression from the paternal allele. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2010]. lac of sum
Disorder MIM:

604149

Disorder Html: Dystonia-11, myoclonic, 159900 (3)





The following SGCE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SGCE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu62302 XM_011516663 PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439.00
OHu62303 XM_011516664 PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu41837 XM_005250675 PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu62304 XM_011516665 PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu41838 XM_005250677 PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu62305 XM_011516666 PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu62306 XM_011516667 PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu62307 XM_011516668 PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu62308 XM_011516669 PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu41841 NM_001301139 Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu25888 NM_003919 Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379.00
OHu25902 NM_001099400 Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379.00
OHu25818 NM_001099401 Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $389.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu62302
Accession Version XM_011516663.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1512bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product epsilon-sarcoglycan isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCAATTGC CCCGGTGGTG GGAGCTGGGA GACCCCTGTG CTTGGACGGG ACAGGGTCGG 
GGGACACGCA GGATGAGCCC CGCGACCACT GGCACATTCT TGCTGACAGC TTTTTTATTA
AGCTGTGCTG ATGGCATAAA TGGAACAGTT AACTGGAAGA CCAGACAGGC CAACAATTTT
ATTATCAGCA GAAAAATGGC TGCTGGGATG AAAGATGTGT ACAGTATTTT CTCCAAGGTA
CACTCCGATC GGAATGTATA CCCATCAGCA GGTGTCCTCT TTGTTCATGT TTTGGAAAGA
GAATATTTTA AGGGGGAATT TCCACCTTAC CCAAAACCTG GCGAGATTAG TAATGATCCC
ATAACATTTA ATACAAATTT AATGGGTTAC CCAGACCGAC CTGGATGGCT TCGATATATC
CAAAGGACAC CATATAGTGA TGGAGTCCTA TATGGGTCCC CAACAGCTGA AAATGTGGGG
AAGCCAACAA TCATTGAGAT AACTGCCTAC AACAGGCGCA CCTTTGAGAC TGCAAGGCAT
AATTTGATAA TTAATATAAT GTCTGCAGAA GACTTCCCGT TGCCATATCA AGCAGAATTC
TTCATTAAGA ATATGAATGT AGAAGAAATG TTGGCCAGTG AGGTTCTTGG AGACTTTCTT
GGCGCAGTGA AAAATGTGTG GCAGCCAGAG CGCCTGAACG CCATAAACAT CACATCGGCC
CTAGACAGGG GTGGCAGGGT GCCACTTCCC ATTAATGACC TGAAGGAGGG CGTTTATGTC
ATGGTTGGTG CAGATGTCCC GTTTTCTTCT TGTTTACGAG AAGTTGAAAA TCCACAGAAT
CAATTGAGAT GTAGTCAAGA AATGGAGCCT GTAATAACAT GTGATAAAAA ATTTCGTACT
CAATTTTACA TTGACTGGTG CAAAATTTCA TTGGTTGATA AAACAAAGCA AGTGTCCACC
TATCAGGAAG TGATTCGTGG AGAGGGGATT TTACCTGATG GTGGAGAATA CAAACCCCCT
TCTGATTCTT TGAAAAGCAG AGACTATTAC ACGGATTTCC TAATTACACT GGCTGTGCCC
TCGGCAGTGG CACTGGTCCT TTTTCTAATA CTTGCTTATA TCATGTGCTG CCGACGGGAA
GGCGTGGAAA AGAGAAACAT GCAAACACCA GACATCCAAC TGGTCCATCA CAGTGCTATT
CAGAAATCTA CCAAGGAGCT TCGAGACATG TCCAAGAATA GAGAGATAGC ATGGCCCCTG
TCAACGCTTC CTGTGTTCCA CCCTGTGACT GGGGAAATCA TACCTCCTTT ACACACAGAC
AACTATGATA GCACAAACAT GCCATTGATG CAAACGCAGC AGAACTTGCC ACATCAGACT
CAGATTCCCC AACAGCAGAC TACAGGAGAT TTTCGTTTGA CAACTTTTCA AAGATTTGAG
GCAAGTATAA TGAAATTGTT CATCAGATTT CAATTACAAG ATGTTTATCA GAATTTTTCC
CTACAATTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011514965.1
CDS84..1595
Misc Feature(1)300..1445(+)
Misc Feature(2)342..656(+)
Misc Feature(3)351..554(+)
Translation
Position Chain Variation Link
35 35 a, t dbSNP:776543055
48 48 a, g dbSNP:768305737
51 51 -, gggag dbSNP:534632849
51 51 a, g dbSNP:760407098
52 52 -, ggaga dbSNP:777001642
55 55 c, t dbSNP:775235692
62 62 c, g, t dbSNP:745436141
63 63 c, g dbSNP:773984458
64 64 a, c dbSNP:771517384
66 66 a, g dbSNP:749727444
77 77 a, g dbSNP:778226570
80 80 a, g dbSNP:756382042
85 85 c, t dbSNP:748379190
87 87 a, c dbSNP:781322593
88 88 a, c dbSNP:755191122
92 92 g, t dbSNP:751698983
94 94 c, t dbSNP:372207991
97 97 g, t dbSNP:147148588
104 104 a, g dbSNP:201378067
116 116 c, t dbSNP:753678586
120 120 -, a dbSNP:766778442
123 123 a, g dbSNP:763973436
127 127 c, g dbSNP:760330361
133 133 g, t dbSNP:373127563
138 138 a, g dbSNP:767335346
142 142 a, g dbSNP:759241679
146 146 -, g dbSNP:760824608
150 150 c, g dbSNP:774045225
151 151 g, t dbSNP:770302911
157 157 a, t dbSNP:749820152
160 160 a, g dbSNP:773531358
167 167 g, t dbSNP:770197127
176 176 c, t dbSNP:748581376
211 211 c, t dbSNP:761677071
220 220 c, t dbSNP:142744182
241 241 a, c, g dbSNP:116324366
248 248 a, g dbSNP:768630572
262 262 c, t dbSNP:534807014
282 282 a, g dbSNP:565647846
301 301 c, t dbSNP:369006527
306 306 a, t dbSNP:751226572
309 309 a, g dbSNP:766011440
316 316 c, t dbSNP:762656646
324 324 c, t dbSNP:749953335
325 325 a, c dbSNP:764776893
329 329 c, t dbSNP:116632777
330 330 a, g dbSNP:199787155
333 333 a, c, t dbSNP:760964336
334 334 a, g dbSNP:148483382
337 337 a, g dbSNP:11548284
338 338 g, t dbSNP:772230342
347 347 a, g dbSNP:746070224
360 360 -, caggtgtcc dbSNP:773475595
360 360 c, t dbSNP:778890137
362 362 c, t dbSNP:771086531
363 363 c, t dbSNP:748106020
382 382 c, g dbSNP:766866819
396 396 a, g dbSNP:754959062
400 400 a, g dbSNP:751366329
406 406 c, t dbSNP:779878703
409 409 c, t dbSNP:758030589
410 410 c, t dbSNP:750056501
425 425 c, t dbSNP:768766085
426 426 a, g dbSNP:375983969
428 428 c, g dbSNP:144527028
432 432 a, g dbSNP:758164053
465 465 a, g dbSNP:751883227
468 468 g, t dbSNP:149295657
469 469 a, g dbSNP:778474711
470 470 c, t dbSNP:756901549
480 480 c, t dbSNP:121908489
481 481 a, g dbSNP:11548285
485 485 c, t dbSNP:138616225
495 495 c, t dbSNP:121908490
496 496 a, g dbSNP:753195301
499 499 a, g dbSNP:200979763
509 509 a, g dbSNP:767782290
511 511 c, t dbSNP:759877587
515 515 a, g dbSNP:554784851
519 519 a, g dbSNP:370382282
547 547 a, c dbSNP:766764222
558 558 a, g dbSNP:369755440
560 560 c, g dbSNP:140913016
561 561 a, g dbSNP:142076876
569 569 a, c dbSNP:746942662
575 575 c, t dbSNP:138794751
578 578 c, t dbSNP:775439702
582 582 a, g dbSNP:370609227
586 586 c, t dbSNP:772108295
600 600 c, t dbSNP:557861177
601 601 a, g, t dbSNP:548306335
604 604 c, g, t dbSNP:777335847
614 614 -, act dbSNP:539327067
615 615 a, g dbSNP:769326027
619 619 a, g dbSNP:747750684
620 620 g, t dbSNP:145010266
627 627 a, c, t dbSNP:752074255
636 636 a, g dbSNP:780589840
640 640 c, t dbSNP:572107071
643 643 a, t dbSNP:750679376
647 647 c, t dbSNP:765566124
661 661 c, t dbSNP:768233445
662 662 a, g dbSNP:747531940
675 675 a, g dbSNP:780640982
678 678 g, t dbSNP:758973154
689 689 g, t dbSNP:746296901
691 691 a, c dbSNP:779127500
695 695 a, t dbSNP:757542604
696 696 a, g dbSNP:753955515
698 698 g, t dbSNP:764244520
701 701 c, t dbSNP:55853245
712 712 c, t dbSNP:752764608
724 724 a, c dbSNP:766361503
726 726 g, t dbSNP:762854508
729 729 a, c dbSNP:376936851
731 731 c, t dbSNP:199727473
741 741 c, t dbSNP:761551499
743 743 c, t dbSNP:776091871
746 746 c, t dbSNP:768286622
747 747 a, g dbSNP:746585235
770 770 a, g dbSNP:148169153
774 774 c, t dbSNP:143839858
775 775 a, g dbSNP:746331571
778 778 g, t dbSNP:121908491
782 782 c, t dbSNP:372968907
783 783 a, g, t dbSNP:749526870
788 788 a, g dbSNP:777920643
797 797 a, c, g dbSNP:148979783
800 800 a, g, t dbSNP:779674432
808 808 a, g dbSNP:727504150
824 824 a, g dbSNP:758362726
827 827 a, g dbSNP:750280111
850 850 a, t dbSNP:765095895
851 851 c, g dbSNP:369157830
854 854 c, t dbSNP:781270494
855 855 a, g dbSNP:754956483
861 861 a, g dbSNP:374149816
865 865 c, t dbSNP:549213726
880 880 c, t dbSNP:201158658
883 883 c, t dbSNP:755996611
884 884 a, g dbSNP:757206832
886 886 c, t dbSNP:200912914
900 900 c, t dbSNP:398123812
903 903 a, c, g dbSNP:200035109
907 907 c, t dbSNP:142833106
923 923 g, t dbSNP:760437933
927 927 c, t dbSNP:752351941
929 929 a, g dbSNP:767137229
954 954 a, g dbSNP:759032005
955 955 c, t dbSNP:773893939
960 960 a, c dbSNP:116105264
974 974 a, g dbSNP:763484333
978 978 c, t dbSNP:199871508
979 979 a, g, t dbSNP:375899729
990 990 a, t dbSNP:781326840
1003 1003 a, g dbSNP:372686312
1012 1012 c, t dbSNP:747116474
1014 1014 c, t dbSNP:780163710
1027 1027 c, t dbSNP:747227969
1033 1033 a, g dbSNP:200400681
1046 1046 c, t dbSNP:201234561
1047 1047 c, g dbSNP:775468466
1055 1055 c, g dbSNP:772144979
1059 1059 a, c, g, t dbSNP:576417569
1060 1060 a, g dbSNP:748001731
1070 1070 a, g dbSNP:781005887
1071 1071 a, t dbSNP:556458255
1075 1075 c, t dbSNP:142079200
1077 1077 c, t dbSNP:375828258
1083 1083 a, g dbSNP:757835571
1084 1084 a, g dbSNP:749932459
1086 1086 a, g dbSNP:765792098
1090 1090 a, c dbSNP:11548286
1096 1096 a, t dbSNP:762287154
1098 1098 c, t dbSNP:777023291
1100 1100 c, t dbSNP:764483578
1119 1119 a, g dbSNP:760832014
1125 1125 a, g dbSNP:775727447
1128 1128 c, t dbSNP:774701123
1130 1130 c, t dbSNP:772177078
1136 1136 a, g dbSNP:539333988
1151 1151 a, c dbSNP:774546647
1157 1157 a, t dbSNP:769888226
1158 1158 a, g dbSNP:147550814
1160 1160 a, g dbSNP:780870614
1164 1164 g, t dbSNP:754742075
1165 1165 c, t dbSNP:559353446
1166 1166 a, g dbSNP:145040065
1187 1187 c, t dbSNP:779825167
1189 1189 c, t dbSNP:758035606
1200 1200 c, t dbSNP:749985422
1216 1216 a, g dbSNP:764696852
1218 1218 c, t dbSNP:757796461
1219 1219 a, g dbSNP:755083075
1220 1220 g, t dbSNP:764383967
1224 1224 a, g dbSNP:553677309
1227 1227 a, g dbSNP:533652380
1230 1230 c, g dbSNP:756703589
1234 1234 a, g dbSNP:754309608
1246 1246 a, g dbSNP:186919286
1249 1249 -, cacca dbSNP:758213105
1252 1252 c, t dbSNP:778398331
1253 1253 a, g dbSNP:756552102
1263 1263 c, t dbSNP:139062360
1292 1292 a, t dbSNP:756672671
1297 1297 a, c dbSNP:753092622
1300 1300 a, g dbSNP:781699232
1305 1305 c, t dbSNP:121908492
1306 1306 a, g dbSNP:373904456
1312 1312 c, t dbSNP:751967746
1319 1319 a, g dbSNP:766764410
1320 1320 a, c dbSNP:763064939
1326 1326 a, g dbSNP:750556405
1330 1330 g, t dbSNP:765193255
1340 1340 c, g dbSNP:760687034
1343 1343 c, g dbSNP:775351067
1346 1346 a, g dbSNP:767308451
1347 1347 a, g dbSNP:759396645
1348 1348 c, g, t dbSNP:370800943
1349 1349 a, g dbSNP:368276611
1356 1356 c, g dbSNP:374697567
1367 1367 c, t dbSNP:769125810
1368 1368 a, g dbSNP:150797717
1369 1369 c, t dbSNP:200020922
1377 1377 g, t dbSNP:781781262
1379 1379 a, g dbSNP:755528173
1386 1386 c, t dbSNP:747427574
1387 1387 a, c, t dbSNP:17851923
1397 1397 c, t dbSNP:758680153
1408 1408 a, g dbSNP:148660601
1417 1417 c, t dbSNP:545029310
1428 1428 c, t dbSNP:147387740
1438 1438 c, t dbSNP:560154922
1439 1439 a, c, g dbSNP:759435338
1458 1458 c, t dbSNP:766199553
1466 1466 c, g dbSNP:762720842
1472 1472 c, g, t dbSNP:148126317
1476 1476 c, g dbSNP:371568101
1481 1481 a, g dbSNP:370115639
1485 1485 a, g dbSNP:776313613
1498 1498 a, g dbSNP:370584439
1519 1519 g, t dbSNP:763703803
1524 1524 g, t dbSNP:760331718
1539 1539 c, t dbSNP:530763000
1541 1541 g, t dbSNP:775056533
1557 1557 c, t dbSNP:772509228
1587 1587 c, t dbSNP:188711194
1589 1589 a, g dbSNP:551544662
1623 1623 a, g dbSNP:767828846
1640 1640 c, g dbSNP:759942828
1644 1644 a, g dbSNP:528155494
1671 1671 -, a dbSNP:764856244
1720 1720 a, c dbSNP:559397123
1737 1737 a, c dbSNP:775004588
1752 1752 a, t dbSNP:562232172
1803 1803 a, t dbSNP:542582678
1875 1875 a, t dbSNP:149413332
2010 2010 c, g dbSNP:564045029
2011 2011 a, g dbSNP:184591117
2025 2025 a, g dbSNP:577854696
2027 2027 a, g dbSNP:550409736
2038 2038 a, g dbSNP:181929444
2068 2068 c, g dbSNP:207468289
2115 2115 a, c dbSNP:138932929
2122 2122 g, t dbSNP:572179315
2159 2159 a, c dbSNP:756204577
2186 2186 a, c dbSNP:117713497
2196 2196 a, g dbSNP:10224050
2237 2237 c, t dbSNP:368212965
2246 2246 a, g dbSNP:761432661
2291 2291 g, t dbSNP:569930848
2298 2298 c, t dbSNP:189873533
2316 2316 a, g dbSNP:374795955
2332 2332 a, g dbSNP:536937536
2338 2338 a, c dbSNP:571477191
2341 2341 -, ac dbSNP:34311900
2344 2344 -, ca dbSNP:200947714
2383 2383 -, tagt dbSNP:570295132
2503 2503 c, t dbSNP:1851422
2509 2509 c, t dbSNP:528220025
2594 2594 c, t dbSNP:11770181
2595 2595 a, g dbSNP:185746195
2629 2629 -, a dbSNP:397945128
2634 2634 -, a dbSNP:58533148
2635 2635 c, t dbSNP:528928324
2676 2676 c, t dbSNP:545463863
2678 2678 c, g dbSNP:746695033
2685 2685 a, g dbSNP:779747909
2690 2690 a, g dbSNP:527307225
2714 2714 a, g dbSNP:192622243
2719 2719 g, t dbSNP:146910891
2757 2757 c, t dbSNP:11770125
2777 2777 c, t dbSNP:11770122
2784 2784 a, g dbSNP:187795375
2799 2799 -, a dbSNP:201939274
2799 2799 a, g dbSNP:183955857
2822 2822 a, g dbSNP:771766275
2865 2865 c, t dbSNP:541191572
2880 2880 c, t dbSNP:565793555
2891 2891 a, g dbSNP:751940135
2896 2896 a, g dbSNP:537403420
2924 2924 c, t dbSNP:73425436
2952 2952 a, t dbSNP:745601660
2967 2967 a, g dbSNP:555856232
2973 2973 a, g dbSNP:541956695
3013 3013 c, g dbSNP:547583586
3066 3066 g, t dbSNP:191381456
3083 3083 c, t dbSNP:188293027
3085 3085 c, t dbSNP:778842632
3094 3094 a, t dbSNP:538851719
3137 3137 a, g dbSNP:537346506
3164 3164 c, t dbSNP:753743424
3168 3168 c, t dbSNP:114647036

Target ORF information:

RefSeq Version XM_011516663
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516663

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCAATTGC CCCGGTGGTG GGAGCTGGGA GACCCCTGTG CTTGGACGGG ACAGGGTCGG 
GGGACACGCA GGATGAGCCC CGCGACCACT GGCACATTCT TGCTGACAGC TTTTTTATTA
AGCTGTGCTG ATGGCATAAA TGGAACAGTT AACTGGAAGA CCAGACAGGC CAACAATTTT
ATTATCAGCA GAAAAATGGC TGCTGGGATG AAAGATGTGT ACAGTATTTT CTCCAAGGTA
CACTCCGATC GGAATGTATA CCCATCAGCA GGTGTCCTCT TTGTTCATGT TTTGGAAAGA
GAATATTTTA AGGGGGAATT TCCACCTTAC CCAAAACCTG GCGAGATTAG TAATGATCCC
ATAACATTTA ATACAAATTT AATGGGTTAC CCAGACCGAC CTGGATGGCT TCGATATATC
CAAAGGACAC CATATAGTGA TGGAGTCCTA TATGGGTCCC CAACAGCTGA AAATGTGGGG
AAGCCAACAA TCATTGAGAT AACTGCCTAC AACAGGCGCA CCTTTGAGAC TGCAAGGCAT
AATTTGATAA TTAATATAAT GTCTGCAGAA GACTTCCCGT TGCCATATCA AGCAGAATTC
TTCATTAAGA ATATGAATGT AGAAGAAATG TTGGCCAGTG AGGTTCTTGG AGACTTTCTT
GGCGCAGTGA AAAATGTGTG GCAGCCAGAG CGCCTGAACG CCATAAACAT CACATCGGCC
CTAGACAGGG GTGGCAGGGT GCCACTTCCC ATTAATGACC TGAAGGAGGG CGTTTATGTC
ATGGTTGGTG CAGATGTCCC GTTTTCTTCT TGTTTACGAG AAGTTGAAAA TCCACAGAAT
CAATTGAGAT GTAGTCAAGA AATGGAGCCT GTAATAACAT GTGATAAAAA ATTTCGTACT
CAATTTTACA TTGACTGGTG CAAAATTTCA TTGGTTGATA AAACAAAGCA AGTGTCCACC
TATCAGGAAG TGATTCGTGG AGAGGGGATT TTACCTGATG GTGGAGAATA CAAACCCCCT
TCTGATTCTT TGAAAAGCAG AGACTATTAC ACGGATTTCC TAATTACACT GGCTGTGCCC
TCGGCAGTGG CACTGGTCCT TTTTCTAATA CTTGCTTATA TCATGTGCTG CCGACGGGAA
GGCGTGGAAA AGAGAAACAT GCAAACACCA GACATCCAAC TGGTCCATCA CAGTGCTATT
CAGAAATCTA CCAAGGAGCT TCGAGACATG TCCAAGAATA GAGAGATAGC ATGGCCCCTG
TCAACGCTTC CTGTGTTCCA CCCTGTGACT GGGGAAATCA TACCTCCTTT ACACACAGAC
AACTATGATA GCACAAACAT GCCATTGATG CAAACGCAGC AGAACTTGCC ACATCAGACT
CAGATTCCCC AACAGCAGAC TACAGGAGAT TTTCGTTTGA CAACTTTTCA AAGATTTGAG
GCAAGTATAA TGAAATTGTT CATCAGATTT CAATTACAAG ATGTTTATCA GAATTTTTCC
CTACAATTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62303
Accession Version XM_011516664.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1485bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product epsilon-sarcoglycan isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAATTGC CCCGGTGGTG GGAGCTGGGA GACCCCTGTG CTTGGACGGG ACAGGGTCGG 
GGGACACGCA GGATGAGCCC CGCGACCACT GGCACATTCT TGCTGACAGC TTTTTTATTA
AGCTGTGCTG ATGGCATAAA TGGAACAGTT AACTGGAAGA CCAGACAGGC CAACAATTTT
ATTATCAGCA GAAAAATGGC TGCTGGGATG AAAGATGTGT ACAGTATTTT CTCCAAGGTA
CACTCCGATC GGAATGTATA CCCATCAGCA GGTGTCCTCT TTGTTCATGT TTTGGAAAGA
GAATATTTTA AGGGGGAATT TCCACCTTAC CCAAAACCTG GCGAGATTAG TAATGATCCC
ATAACATTTA ATACAAATTT AATGGGTTAC CCAGACCGAC CTGGATGGCT TCGATATATC
CAAAGGACAC CATATAGTGA TGGAGTCCTA TATGGGTCCC CAACAGCTGA AAATGTGGGG
AAGCCAACAA TCATTGAGAT AACTGCCTAC AACAGGCGCA CCTTTGAGAC TGCAAGGCAT
AATTTGATAA TTAATATAAT GTCTGCAGAA GACTTCCCGT TGCCATATCA AGCAGAATTC
TTCATTAAGA ATATGAATGT AGAAGAAATG TTGGCCAGTG AGGTTCTTGG AGACTTTCTT
GGCGCAGTGA AAAATGTGTG GCAGCCAGAG CGCCTGAACG CCATAAACAT CACATCGGCC
CTAGACAGGG GTGGCAGGGT GCCACTTCCC ATTAATGACC TGAAGGAGGG CGTTTATGTC
ATGGTTGGTG CAGATGTCCC GTTTTCTTCT TGTTTACGAG AAGTTGAAAA TCCACAGAAT
CAATTGAGAT GTAGTCAAGA AATGGAGCCT GTAATAACAT GTGATAAAAA ATTTCGTACT
CAATTTTACA TTGACTGGTG CAAAATTTCA TTGGTTGATA AAACAAAGCA AGTGTCCACC
TATCAGGAAG TGATTCGTGG AGAGGGGATT TTACCTGATG GTGGAGAATA CAAACCCCCT
TCTGATTCTT TGAAAAGCAG AGACTATTAC ACGGATTTCC TAATTACACT GGCTGTGCCC
TCGGCAGTGG CACTGGTCCT TTTTCTAATA CTTGCTTATA TCATGTGCTG CCGACGGGAA
GGCGTCATCC AACTGGTCCA TCACAGTGCT ATTCAGAAAT CTACCAAGGA GCTTCGAGAC
ATGTCCAAGA ATAGAGAGAT AGCATGGCCC CTGTCAACGC TTCCTGTGTT CCACCCTGTG
ACTGGGGAAA TCATACCTCC TTTACACACA GACAACTATG ATAGCACAAA CATGCCATTG
ATGCAAACGC AGCAGAACTT GCCACATCAG ACTCAGATTC CCCAACAGCA GACTACAGGA
GATTTTCGTT TGACAACTTT TCAAAGATTT GAGGCAAGTA TAATGAAATT GTTCATCAGA
TTTCAATTAC AAGATGTTTA TCAGAATTTT TCCCTACAAT TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011514966.1
CDS84..1568
Misc Feature(1)300..1418(+)
Misc Feature(2)342..656(+)
Misc Feature(3)351..554(+)
Translation
Position Chain Variation Link
35 35 a, t dbSNP:776543055
48 48 a, g dbSNP:768305737
51 51 -, gggag dbSNP:534632849
51 51 a, g dbSNP:760407098
52 52 -, ggaga dbSNP:777001642
55 55 c, t dbSNP:775235692
62 62 c, g, t dbSNP:745436141
63 63 c, g dbSNP:773984458
64 64 a, c dbSNP:771517384
66 66 a, g dbSNP:749727444
77 77 a, g dbSNP:778226570
80 80 a, g dbSNP:756382042
85 85 c, t dbSNP:748379190
87 87 a, c dbSNP:781322593
88 88 a, c dbSNP:755191122
92 92 g, t dbSNP:751698983
94 94 c, t dbSNP:372207991
97 97 g, t dbSNP:147148588
104 104 a, g dbSNP:201378067
116 116 c, t dbSNP:753678586
120 120 -, a dbSNP:766778442
123 123 a, g dbSNP:763973436
127 127 c, g dbSNP:760330361
133 133 g, t dbSNP:373127563
138 138 a, g dbSNP:767335346
142 142 a, g dbSNP:759241679
146 146 -, g dbSNP:760824608
150 150 c, g dbSNP:774045225
151 151 g, t dbSNP:770302911
157 157 a, t dbSNP:749820152
160 160 a, g dbSNP:773531358
167 167 g, t dbSNP:770197127
176 176 c, t dbSNP:748581376
211 211 c, t dbSNP:761677071
220 220 c, t dbSNP:142744182
241 241 a, c, g dbSNP:116324366
248 248 a, g dbSNP:768630572
262 262 c, t dbSNP:534807014
282 282 a, g dbSNP:565647846
301 301 c, t dbSNP:369006527
306 306 a, t dbSNP:751226572
309 309 a, g dbSNP:766011440
316 316 c, t dbSNP:762656646
324 324 c, t dbSNP:749953335
325 325 a, c dbSNP:764776893
329 329 c, t dbSNP:116632777
330 330 a, g dbSNP:199787155
333 333 a, c, t dbSNP:760964336
334 334 a, g dbSNP:148483382
337 337 a, g dbSNP:11548284
338 338 g, t dbSNP:772230342
347 347 a, g dbSNP:746070224
360 360 -, caggtgtcc dbSNP:773475595
360 360 c, t dbSNP:778890137
362 362 c, t dbSNP:771086531
363 363 c, t dbSNP:748106020
382 382 c, g dbSNP:766866819
396 396 a, g dbSNP:754959062
400 400 a, g dbSNP:751366329
406 406 c, t dbSNP:779878703
409 409 c, t dbSNP:758030589
410 410 c, t dbSNP:750056501
425 425 c, t dbSNP:768766085
426 426 a, g dbSNP:375983969
428 428 c, g dbSNP:144527028
432 432 a, g dbSNP:758164053
465 465 a, g dbSNP:751883227
468 468 g, t dbSNP:149295657
469 469 a, g dbSNP:778474711
470 470 c, t dbSNP:756901549
480 480 c, t dbSNP:121908489
481 481 a, g dbSNP:11548285
485 485 c, t dbSNP:138616225
495 495 c, t dbSNP:121908490
496 496 a, g dbSNP:753195301
499 499 a, g dbSNP:200979763
509 509 a, g dbSNP:767782290
511 511 c, t dbSNP:759877587
515 515 a, g dbSNP:554784851
519 519 a, g dbSNP:370382282
547 547 a, c dbSNP:766764222
558 558 a, g dbSNP:369755440
560 560 c, g dbSNP:140913016
561 561 a, g dbSNP:142076876
569 569 a, c dbSNP:746942662
575 575 c, t dbSNP:138794751
578 578 c, t dbSNP:775439702
582 582 a, g dbSNP:370609227
586 586 c, t dbSNP:772108295
600 600 c, t dbSNP:557861177
601 601 a, g, t dbSNP:548306335
604 604 c, g, t dbSNP:777335847
614 614 -, act dbSNP:539327067
615 615 a, g dbSNP:769326027
619 619 a, g dbSNP:747750684
620 620 g, t dbSNP:145010266
627 627 a, c, t dbSNP:752074255
636 636 a, g dbSNP:780589840
640 640 c, t dbSNP:572107071
643 643 a, t dbSNP:750679376
647 647 c, t dbSNP:765566124
661 661 c, t dbSNP:768233445
662 662 a, g dbSNP:747531940
675 675 a, g dbSNP:780640982
678 678 g, t dbSNP:758973154
689 689 g, t dbSNP:746296901
691 691 a, c dbSNP:779127500
695 695 a, t dbSNP:757542604
696 696 a, g dbSNP:753955515
698 698 g, t dbSNP:764244520
701 701 c, t dbSNP:55853245
712 712 c, t dbSNP:752764608
724 724 a, c dbSNP:766361503
726 726 g, t dbSNP:762854508
729 729 a, c dbSNP:376936851
731 731 c, t dbSNP:199727473
741 741 c, t dbSNP:761551499
743 743 c, t dbSNP:776091871
746 746 c, t dbSNP:768286622
747 747 a, g dbSNP:746585235
770 770 a, g dbSNP:148169153
774 774 c, t dbSNP:143839858
775 775 a, g dbSNP:746331571
778 778 g, t dbSNP:121908491
782 782 c, t dbSNP:372968907
783 783 a, g, t dbSNP:749526870
788 788 a, g dbSNP:777920643
797 797 a, c, g dbSNP:148979783
800 800 a, g, t dbSNP:779674432
808 808 a, g dbSNP:727504150
824 824 a, g dbSNP:758362726
827 827 a, g dbSNP:750280111
850 850 a, t dbSNP:765095895
851 851 c, g dbSNP:369157830
854 854 c, t dbSNP:781270494
855 855 a, g dbSNP:754956483
861 861 a, g dbSNP:374149816
865 865 c, t dbSNP:549213726
880 880 c, t dbSNP:201158658
883 883 c, t dbSNP:755996611
884 884 a, g dbSNP:757206832
886 886 c, t dbSNP:200912914
900 900 c, t dbSNP:398123812
903 903 a, c, g dbSNP:200035109
907 907 c, t dbSNP:142833106
923 923 g, t dbSNP:760437933
927 927 c, t dbSNP:752351941
929 929 a, g dbSNP:767137229
954 954 a, g dbSNP:759032005
955 955 c, t dbSNP:773893939
960 960 a, c dbSNP:116105264
974 974 a, g dbSNP:763484333
978 978 c, t dbSNP:199871508
979 979 a, g, t dbSNP:375899729
990 990 a, t dbSNP:781326840
1003 1003 a, g dbSNP:372686312
1012 1012 c, t dbSNP:747116474
1014 1014 c, t dbSNP:780163710
1027 1027 c, t dbSNP:747227969
1033 1033 a, g dbSNP:200400681
1046 1046 c, t dbSNP:201234561
1047 1047 c, g dbSNP:775468466
1055 1055 c, g dbSNP:772144979
1059 1059 a, c, g, t dbSNP:576417569
1060 1060 a, g dbSNP:748001731
1070 1070 a, g dbSNP:781005887
1071 1071 a, t dbSNP:556458255
1075 1075 c, t dbSNP:142079200
1077 1077 c, t dbSNP:375828258
1083 1083 a, g dbSNP:757835571
1084 1084 a, g dbSNP:749932459
1086 1086 a, g dbSNP:765792098
1090 1090 a, c dbSNP:11548286
1096 1096 a, t dbSNP:762287154
1098 1098 c, t dbSNP:777023291
1100 1100 c, t dbSNP:764483578
1119 1119 a, g dbSNP:760832014
1125 1125 a, g dbSNP:775727447
1128 1128 c, t dbSNP:774701123
1130 1130 c, t dbSNP:772177078
1136 1136 a, g dbSNP:539333988
1151 1151 a, c dbSNP:774546647
1157 1157 a, t dbSNP:769888226
1158 1158 a, g dbSNP:147550814
1160 1160 a, g dbSNP:780870614
1164 1164 g, t dbSNP:754742075
1165 1165 c, t dbSNP:559353446
1166 1166 a, g dbSNP:145040065
1187 1187 c, t dbSNP:779825167
1189 1189 c, t dbSNP:758035606
1200 1200 c, t dbSNP:749985422
1216 1216 a, g dbSNP:764696852
1218 1218 c, t dbSNP:757796461
1219 1219 a, g dbSNP:755083075
1220 1220 g, t dbSNP:764383967
1224 1224 a, g dbSNP:553677309
1227 1227 a, g dbSNP:533652380
1236 1236 c, t dbSNP:139062360
1265 1265 a, t dbSNP:756672671
1270 1270 a, c dbSNP:753092622
1273 1273 a, g dbSNP:781699232
1278 1278 c, t dbSNP:121908492
1279 1279 a, g dbSNP:373904456
1285 1285 c, t dbSNP:751967746
1292 1292 a, g dbSNP:766764410
1293 1293 a, c dbSNP:763064939
1299 1299 a, g dbSNP:750556405
1303 1303 g, t dbSNP:765193255
1313 1313 c, g dbSNP:760687034
1316 1316 c, g dbSNP:775351067
1319 1319 a, g dbSNP:767308451
1320 1320 a, g dbSNP:759396645
1321 1321 c, g, t dbSNP:370800943
1322 1322 a, g dbSNP:368276611
1329 1329 c, g dbSNP:374697567
1340 1340 c, t dbSNP:769125810
1341 1341 a, g dbSNP:150797717
1342 1342 c, t dbSNP:200020922
1350 1350 g, t dbSNP:781781262
1352 1352 a, g dbSNP:755528173
1359 1359 c, t dbSNP:747427574
1360 1360 a, c, t dbSNP:17851923
1370 1370 c, t dbSNP:758680153
1381 1381 a, g dbSNP:148660601
1390 1390 c, t dbSNP:545029310
1401 1401 c, t dbSNP:147387740
1411 1411 c, t dbSNP:560154922
1412 1412 a, c, g dbSNP:759435338
1431 1431 c, t dbSNP:766199553
1439 1439 c, g dbSNP:762720842
1445 1445 c, g, t dbSNP:148126317
1449 1449 c, g dbSNP:371568101
1454 1454 a, g dbSNP:370115639
1458 1458 a, g dbSNP:776313613
1471 1471 a, g dbSNP:370584439
1492 1492 g, t dbSNP:763703803
1497 1497 g, t dbSNP:760331718
1512 1512 c, t dbSNP:530763000
1514 1514 g, t dbSNP:775056533
1530 1530 c, t dbSNP:772509228
1560 1560 c, t dbSNP:188711194
1562 1562 a, g dbSNP:551544662
1596 1596 a, g dbSNP:767828846
1613 1613 c, g dbSNP:759942828
1617 1617 a, g dbSNP:528155494
1644 1644 -, a dbSNP:764856244
1693 1693 a, c dbSNP:559397123
1710 1710 a, c dbSNP:775004588
1725 1725 a, t dbSNP:562232172
1776 1776 a, t dbSNP:542582678
1848 1848 a, t dbSNP:149413332
1983 1983 c, g dbSNP:564045029
1984 1984 a, g dbSNP:184591117
1998 1998 a, g dbSNP:577854696
2000 2000 a, g dbSNP:550409736
2011 2011 a, g dbSNP:181929444
2041 2041 c, g dbSNP:207468289
2088 2088 a, c dbSNP:138932929
2095 2095 g, t dbSNP:572179315
2132 2132 a, c dbSNP:756204577
2159 2159 a, c dbSNP:117713497
2169 2169 a, g dbSNP:10224050
2210 2210 c, t dbSNP:368212965
2219 2219 a, g dbSNP:761432661
2264 2264 g, t dbSNP:569930848
2271 2271 c, t dbSNP:189873533
2289 2289 a, g dbSNP:374795955
2305 2305 a, g dbSNP:536937536
2311 2311 a, c dbSNP:571477191
2314 2314 -, ac dbSNP:34311900
2317 2317 -, ca dbSNP:200947714
2356 2356 -, tagt dbSNP:570295132
2476 2476 c, t dbSNP:1851422
2482 2482 c, t dbSNP:528220025
2567 2567 c, t dbSNP:11770181
2568 2568 a, g dbSNP:185746195
2602 2602 -, a dbSNP:397945128
2607 2607 -, a dbSNP:58533148
2608 2608 c, t dbSNP:528928324
2649 2649 c, t dbSNP:545463863
2651 2651 c, g dbSNP:746695033
2658 2658 a, g dbSNP:779747909
2663 2663 a, g dbSNP:527307225
2687 2687 a, g dbSNP:192622243
2692 2692 g, t dbSNP:146910891
2730 2730 c, t dbSNP:11770125
2750 2750 c, t dbSNP:11770122
2757 2757 a, g dbSNP:187795375
2772 2772 -, a dbSNP:201939274
2772 2772 a, g dbSNP:183955857
2795 2795 a, g dbSNP:771766275
2838 2838 c, t dbSNP:541191572
2853 2853 c, t dbSNP:565793555
2864 2864 a, g dbSNP:751940135
2869 2869 a, g dbSNP:537403420
2897 2897 c, t dbSNP:73425436
2925 2925 a, t dbSNP:745601660
2940 2940 a, g dbSNP:555856232
2946 2946 a, g dbSNP:541956695
2986 2986 c, g dbSNP:547583586
3039 3039 g, t dbSNP:191381456
3056 3056 c, t dbSNP:188293027
3058 3058 c, t dbSNP:778842632
3067 3067 a, t dbSNP:538851719
3110 3110 a, g dbSNP:537346506
3137 3137 c, t dbSNP:753743424
3141 3141 c, t dbSNP:114647036
3184 3184 g, t dbSNP:13222972
3187 3187 g, t dbSNP:13307770

Target ORF information:

RefSeq Version XM_011516664
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516664

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAATTGC CCCGGTGGTG GGAGCTGGGA GACCCCTGTG CTTGGACGGG ACAGGGTCGG 
GGGACACGCA GGATGAGCCC CGCGACCACT GGCACATTCT TGCTGACAGC TTTTTTATTA
AGCTGTGCTG ATGGCATAAA TGGAACAGTT AACTGGAAGA CCAGACAGGC CAACAATTTT
ATTATCAGCA GAAAAATGGC TGCTGGGATG AAAGATGTGT ACAGTATTTT CTCCAAGGTA
CACTCCGATC GGAATGTATA CCCATCAGCA GGTGTCCTCT TTGTTCATGT TTTGGAAAGA
GAATATTTTA AGGGGGAATT TCCACCTTAC CCAAAACCTG GCGAGATTAG TAATGATCCC
ATAACATTTA ATACAAATTT AATGGGTTAC CCAGACCGAC CTGGATGGCT TCGATATATC
CAAAGGACAC CATATAGTGA TGGAGTCCTA TATGGGTCCC CAACAGCTGA AAATGTGGGG
AAGCCAACAA TCATTGAGAT AACTGCCTAC AACAGGCGCA CCTTTGAGAC TGCAAGGCAT
AATTTGATAA TTAATATAAT GTCTGCAGAA GACTTCCCGT TGCCATATCA AGCAGAATTC
TTCATTAAGA ATATGAATGT AGAAGAAATG TTGGCCAGTG AGGTTCTTGG AGACTTTCTT
GGCGCAGTGA AAAATGTGTG GCAGCCAGAG CGCCTGAACG CCATAAACAT CACATCGGCC
CTAGACAGGG GTGGCAGGGT GCCACTTCCC ATTAATGACC TGAAGGAGGG CGTTTATGTC
ATGGTTGGTG CAGATGTCCC GTTTTCTTCT TGTTTACGAG AAGTTGAAAA TCCACAGAAT
CAATTGAGAT GTAGTCAAGA AATGGAGCCT GTAATAACAT GTGATAAAAA ATTTCGTACT
CAATTTTACA TTGACTGGTG CAAAATTTCA TTGGTTGATA AAACAAAGCA AGTGTCCACC
TATCAGGAAG TGATTCGTGG AGAGGGGATT TTACCTGATG GTGGAGAATA CAAACCCCCT
TCTGATTCTT TGAAAAGCAG AGACTATTAC ACGGATTTCC TAATTACACT GGCTGTGCCC
TCGGCAGTGG CACTGGTCCT TTTTCTAATA CTTGCTTATA TCATGTGCTG CCGACGGGAA
GGCGTCATCC AACTGGTCCA TCACAGTGCT ATTCAGAAAT CTACCAAGGA GCTTCGAGAC
ATGTCCAAGA ATAGAGAGAT AGCATGGCCC CTGTCAACGC TTCCTGTGTT CCACCCTGTG
ACTGGGGAAA TCATACCTCC TTTACACACA GACAACTATG ATAGCACAAA CATGCCATTG
ATGCAAACGC AGCAGAACTT GCCACATCAG ACTCAGATTC CCCAACAGCA GACTACAGGA
GATTTTCGTT TGACAACTTT TCAAAGATTT GAGGCAAGTA TAATGAAATT GTTCATCAGA
TTTCAATTAC AAGATGTTTA TCAGAATTTT TCCCTACAAT TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41837
Accession Version XM_005250675.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product epsilon-sarcoglycan isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578814713. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCAATTGC CCCGGTGGTG GGAGCTGGGA GACCCCTGTG CTTGGACGGG ACAGGGTCGG 
GGGACACGCA GGATGAGCCC CGCGACCACT GGCACATTCT TGCTGACAGC TTTTTTATTA
AGCTGTGCTG ATGGCATAAA TGGAACAGTT AACTGGAAGA CCAGACAGGC CAACAATTTT
ATTATCAGCA GAAAAATGGC TGCTGGGATG AAAGATGTGT ACAGTATTTT CTCCAAGGTA
CACTCCGATC GGAATGTATA CCCATCAGCA GGTGTCCTCT TTGTTCATGT TTTGGAAAGA
GAATATTTTA AGGGGGAATT TCCACCTTAC CCAAAACCTG GCGAGATTAG TAATGATCCC
ATAACATTTA ATACAAATTT AATGGGTTAC CCAGACCGAC CTGGATGGCT TCGATATATC
CAAAGGACAC CATATAGTGA TGGAGTCCTA TATGGGTCCC CAACAGCTGA AAATGTGGGG
AAGCCAACAA TCATTGAGAT AACTGCCTAC AACAGGCGCA CCTTTGAGAC TGCAAGGCAT
AATTTGATAA TTAATATAAT GTCTGCAGAA GACTTCCCGT TGCCATATCA AGCAGAATTC
TTCATTAAGA ATATGAATGT AGAAGAAATG TTGGCCAGTG AGGTTCTTGG AGACTTTCTT
GGCGCAGTGA AAAATGTGTG GCAGCCAGAG CGCCTGAACG CCATAAACAT CACATCGGCC
CTAGACAGGG GTGGCAGGGT GCCACTTCCC ATTAATGACC TGAAGGAGGG CGTTTATGTC
ATGGTTGGTG CAGATGTCCC GTTTTCTTCT TGTTTACGAG AAGTTGAAAA TCCACAGAAT
CAATTGAGAT GTAGTCAAGA AATGGAGCCT GTAATAACAT GTGATAAAAA ATTTCGTACT
CAATTTTACA TTGACTGGTG CAAAATTTCA TTGGTTGATA AAACAAAGCA AGTGTCCACC
TATCAGGAAG TGATTCGTGG AGAGGGGATT TTACCTGATG GTGGAGAATA CAAACCCCCT
TCTGATTCTT TGAAAAGCAG AGACTATTAC ACGGATTTCC TAATTACACT GGCTGTGCCC
TCGGCAGTGG CACTGGTCCT TTTTCTAATA CTTGCTTATA TCATGTGCTG CCGACGGGAA
GGCGTGGAAA AGAGAAACAT GCAAACACCA GACATCCAAC TGGTCCATCA CAGTGCTATT
CAGAAATCTA CCAAGGAGCT TCGAGACATG TCCAAGAATA GAGAGATAGC ATGGCCCCTG
TCAACGCTTC CTGTGTTCCA CCCTGTGACT GGGGAAATCA TACCTCCTTT ACACACAGAC
AACTATGATA GCACAAACAT GCCATTGATG CAAACGCAGC AGAACTTGCC ACATCAGACT
CAGATTCCCC AACAGCAGAC TACAGGTAAA TGGTATCCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005250732.1
CDS84..1505
Misc Feature(1)300..1445(+)
Misc Feature(2)342..656(+)
Misc Feature(3)351..554(+)
Translation
Position Chain Variation Link
35 35 a, t dbSNP:776543055
48 48 a, g dbSNP:768305737
51 51 -, gggag dbSNP:534632849
51 51 a, g dbSNP:760407098
52 52 -, ggaga dbSNP:777001642
55 55 c, t dbSNP:775235692
62 62 c, g, t dbSNP:745436141
63 63 c, g dbSNP:773984458
64 64 a, c dbSNP:771517384
66 66 a, g dbSNP:749727444
77 77 a, g dbSNP:778226570
80 80 a, g dbSNP:756382042
85 85 c, t dbSNP:748379190
87 87 a, c dbSNP:781322593
88 88 a, c dbSNP:755191122
92 92 g, t dbSNP:751698983
94 94 c, t dbSNP:372207991
97 97 g, t dbSNP:147148588
104 104 a, g dbSNP:201378067
116 116 c, t dbSNP:753678586
120 120 -, a dbSNP:766778442
123 123 a, g dbSNP:763973436
127 127 c, g dbSNP:760330361
133 133 g, t dbSNP:373127563
138 138 a, g dbSNP:767335346
142 142 a, g dbSNP:759241679
146 146 -, g dbSNP:760824608
150 150 c, g dbSNP:774045225
151 151 g, t dbSNP:770302911
157 157 a, t dbSNP:749820152
160 160 a, g dbSNP:773531358
167 167 g, t dbSNP:770197127
176 176 c, t dbSNP:748581376
211 211 c, t dbSNP:761677071
220 220 c, t dbSNP:142744182
241 241 a, c, g dbSNP:116324366
248 248 a, g dbSNP:768630572
262 262 c, t dbSNP:534807014
282 282 a, g dbSNP:565647846
301 301 c, t dbSNP:369006527
306 306 a, t dbSNP:751226572
309 309 a, g dbSNP:766011440
316 316 c, t dbSNP:762656646
324 324 c, t dbSNP:749953335
325 325 a, c dbSNP:764776893
329 329 c, t dbSNP:116632777
330 330 a, g dbSNP:199787155
333 333 a, c, t dbSNP:760964336
334 334 a, g dbSNP:148483382
337 337 a, g dbSNP:11548284
338 338 g, t dbSNP:772230342
347 347 a, g dbSNP:746070224
360 360 -, caggtgtcc dbSNP:773475595
360 360 c, t dbSNP:778890137
362 362 c, t dbSNP:771086531
363 363 c, t dbSNP:748106020
382 382 c, g dbSNP:766866819
396 396 a, g dbSNP:754959062
400 400 a, g dbSNP:751366329
406 406 c, t dbSNP:779878703
409 409 c, t dbSNP:758030589
410 410 c, t dbSNP:750056501
425 425 c, t dbSNP:768766085
426 426 a, g dbSNP:375983969
428 428 c, g dbSNP:144527028
432 432 a, g dbSNP:758164053
465 465 a, g dbSNP:751883227
468 468 g, t dbSNP:149295657
469 469 a, g dbSNP:778474711
470 470 c, t dbSNP:756901549
480 480 c, t dbSNP:121908489
481 481 a, g dbSNP:11548285
485 485 c, t dbSNP:138616225
495 495 c, t dbSNP:121908490
496 496 a, g dbSNP:753195301
499 499 a, g dbSNP:200979763
509 509 a, g dbSNP:767782290
511 511 c, t dbSNP:759877587
515 515 a, g dbSNP:554784851
519 519 a, g dbSNP:370382282
547 547 a, c dbSNP:766764222
558 558 a, g dbSNP:369755440
560 560 c, g dbSNP:140913016
561 561 a, g dbSNP:142076876
569 569 a, c dbSNP:746942662
575 575 c, t dbSNP:138794751
578 578 c, t dbSNP:775439702
582 582 a, g dbSNP:370609227
586 586 c, t dbSNP:772108295
600 600 c, t dbSNP:557861177
601 601 a, g, t dbSNP:548306335
604 604 c, g, t dbSNP:777335847
614 614 -, act dbSNP:539327067
615 615 a, g dbSNP:769326027
619 619 a, g dbSNP:747750684
620 620 g, t dbSNP:145010266
627 627 a, c, t dbSNP:752074255
636 636 a, g dbSNP:780589840
640 640 c, t dbSNP:572107071
643 643 a, t dbSNP:750679376
647 647 c, t dbSNP:765566124
661 661 c, t dbSNP:768233445
662 662 a, g dbSNP:747531940
675 675 a, g dbSNP:780640982
678 678 g, t dbSNP:758973154
689 689 g, t dbSNP:746296901
691 691 a, c dbSNP:779127500
695 695 a, t dbSNP:757542604
696 696 a, g dbSNP:753955515
698 698 g, t dbSNP:764244520
701 701 c, t dbSNP:55853245
712 712 c, t dbSNP:752764608
724 724 a, c dbSNP:766361503
726 726 g, t dbSNP:762854508
729 729 a, c dbSNP:376936851
731 731 c, t dbSNP:199727473
741 741 c, t dbSNP:761551499
743 743 c, t dbSNP:776091871
746 746 c, t dbSNP:768286622
747 747 a, g dbSNP:746585235
770 770 a, g dbSNP:148169153
774 774 c, t dbSNP:143839858
775 775 a, g dbSNP:746331571
778 778 g, t dbSNP:121908491
782 782 c, t dbSNP:372968907
783 783 a, g, t dbSNP:749526870
788 788 a, g dbSNP:777920643
797 797 a, c, g dbSNP:148979783
800 800 a, g, t dbSNP:779674432
808 808 a, g dbSNP:727504150
824 824 a, g dbSNP:758362726
827 827 a, g dbSNP:750280111
850 850 a, t dbSNP:765095895
851 851 c, g dbSNP:369157830
854 854 c, t dbSNP:781270494
855 855 a, g dbSNP:754956483
861 861 a, g dbSNP:374149816
865 865 c, t dbSNP:549213726
880 880 c, t dbSNP:201158658
883 883 c, t dbSNP:755996611
884 884 a, g dbSNP:757206832
886 886 c, t dbSNP:200912914
900 900 c, t dbSNP:398123812
903 903 a, c, g dbSNP:200035109
907 907 c, t dbSNP:142833106
923 923 g, t dbSNP:760437933
927 927 c, t dbSNP:752351941
929 929 a, g dbSNP:767137229
954 954 a, g dbSNP:759032005
955 955 c, t dbSNP:773893939
960 960 a, c dbSNP:116105264
974 974 a, g dbSNP:763484333
978 978 c, t dbSNP:199871508
979 979 a, g, t dbSNP:375899729
990 990 a, t dbSNP:781326840
1003 1003 a, g dbSNP:372686312
1012 1012 c, t dbSNP:747116474
1014 1014 c, t dbSNP:780163710
1027 1027 c, t dbSNP:747227969
1033 1033 a, g dbSNP:200400681
1046 1046 c, t dbSNP:201234561
1047 1047 c, g dbSNP:775468466
1055 1055 c, g dbSNP:772144979
1059 1059 a, c, g, t dbSNP:576417569
1060 1060 a, g dbSNP:748001731
1070 1070 a, g dbSNP:781005887
1071 1071 a, t dbSNP:556458255
1075 1075 c, t dbSNP:142079200
1077 1077 c, t dbSNP:375828258
1083 1083 a, g dbSNP:757835571
1084 1084 a, g dbSNP:749932459
1086 1086 a, g dbSNP:765792098
1090 1090 a, c dbSNP:11548286
1096 1096 a, t dbSNP:762287154
1098 1098 c, t dbSNP:777023291
1100 1100 c, t dbSNP:764483578
1119 1119 a, g dbSNP:760832014
1125 1125 a, g dbSNP:775727447
1128 1128 c, t dbSNP:774701123
1130 1130 c, t dbSNP:772177078
1136 1136 a, g dbSNP:539333988
1151 1151 a, c dbSNP:774546647
1157 1157 a, t dbSNP:769888226
1158 1158 a, g dbSNP:147550814
1160 1160 a, g dbSNP:780870614
1164 1164 g, t dbSNP:754742075
1165 1165 c, t dbSNP:559353446
1166 1166 a, g dbSNP:145040065
1187 1187 c, t dbSNP:779825167
1189 1189 c, t dbSNP:758035606
1200 1200 c, t dbSNP:749985422
1216 1216 a, g dbSNP:764696852
1218 1218 c, t dbSNP:757796461
1219 1219 a, g dbSNP:755083075
1220 1220 g, t dbSNP:764383967
1224 1224 a, g dbSNP:553677309
1227 1227 a, g dbSNP:533652380
1230 1230 c, g dbSNP:756703589
1234 1234 a, g dbSNP:754309608
1246 1246 a, g dbSNP:186919286
1249 1249 -, cacca dbSNP:758213105
1252 1252 c, t dbSNP:778398331
1253 1253 a, g dbSNP:756552102
1263 1263 c, t dbSNP:139062360
1292 1292 a, t dbSNP:756672671
1297 1297 a, c dbSNP:753092622
1300 1300 a, g dbSNP:781699232
1305 1305 c, t dbSNP:121908492
1306 1306 a, g dbSNP:373904456
1312 1312 c, t dbSNP:751967746
1319 1319 a, g dbSNP:766764410
1320 1320 a, c dbSNP:763064939
1326 1326 a, g dbSNP:750556405
1330 1330 g, t dbSNP:765193255
1340 1340 c, g dbSNP:760687034
1343 1343 c, g dbSNP:775351067
1346 1346 a, g dbSNP:767308451
1347 1347 a, g dbSNP:759396645
1348 1348 c, g, t dbSNP:370800943
1349 1349 a, g dbSNP:368276611
1356 1356 c, g dbSNP:374697567
1367 1367 c, t dbSNP:769125810
1368 1368 a, g dbSNP:150797717
1369 1369 c, t dbSNP:200020922
1377 1377 g, t dbSNP:781781262
1379 1379 a, g dbSNP:755528173
1386 1386 c, t dbSNP:747427574
1387 1387 a, c, t dbSNP:17851923
1397 1397 c, t dbSNP:758680153
1408 1408 a, g dbSNP:148660601
1417 1417 c, t dbSNP:545029310
1428 1428 c, t dbSNP:147387740
1438 1438 c, t dbSNP:560154922
1439 1439 a, c, g dbSNP:759435338
1458 1458 c, t dbSNP:766199553
1466 1466 c, g dbSNP:762720842
1472 1472 c, g, t dbSNP:148126317
1476 1476 c, g dbSNP:371568101
1481 1481 a, g dbSNP:370115639
1485 1485 a, g dbSNP:776313613
1489 1489 a, g dbSNP:750326561
1501 1501 c, t dbSNP:778866625
1517 1517 g, t dbSNP:757047752
1535 1535 c, t dbSNP:753684985
1542 1542 a, c dbSNP:760109715
1606 1606 a, g dbSNP:193172306
1660 1660 c, g dbSNP:527929196
1677 1677 c, t dbSNP:112954947
1701 1701 c, t dbSNP:558194543
1709 1709 a, g dbSNP:542318820
1715 1715 c, t dbSNP:188526246
1767 1767 -, tta dbSNP:532244072
1777 1777 c, t dbSNP:11984148

Target ORF information:

RefSeq Version XM_005250675
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005250675

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCAATTGC CCCGGTGGTG GGAGCTGGGA GACCCCTGTG CTTGGACGGG ACAGGGTCGG 
GGGACACGCA GGATGAGCCC CGCGACCACT GGCACATTCT TGCTGACAGC TTTTTTATTA
AGCTGTGCTG ATGGCATAAA TGGAACAGTT AACTGGAAGA CCAGACAGGC CAACAATTTT
ATTATCAGCA GAAAAATGGC TGCTGGGATG AAAGATGTGT ACAGTATTTT CTCCAAGGTA
CACTCCGATC GGAATGTATA CCCATCAGCA GGTGTCCTCT TTGTTCATGT TTTGGAAAGA
GAATATTTTA AGGGGGAATT TCCACCTTAC CCAAAACCTG GCGAGATTAG TAATGATCCC
ATAACATTTA ATACAAATTT AATGGGTTAC CCAGACCGAC CTGGATGGCT TCGATATATC
CAAAGGACAC CATATAGTGA TGGAGTCCTA TATGGGTCCC CAACAGCTGA AAATGTGGGG
AAGCCAACAA TCATTGAGAT AACTGCCTAC AACAGGCGCA CCTTTGAGAC TGCAAGGCAT
AATTTGATAA TTAATATAAT GTCTGCAGAA GACTTCCCGT TGCCATATCA AGCAGAATTC
TTCATTAAGA ATATGAATGT AGAAGAAATG TTGGCCAGTG AGGTTCTTGG AGACTTTCTT
GGCGCAGTGA AAAATGTGTG GCAGCCAGAG CGCCTGAACG CCATAAACAT CACATCGGCC
CTAGACAGGG GTGGCAGGGT GCCACTTCCC ATTAATGACC TGAAGGAGGG CGTTTATGTC
ATGGTTGGTG CAGATGTCCC GTTTTCTTCT TGTTTACGAG AAGTTGAAAA TCCACAGAAT
CAATTGAGAT GTAGTCAAGA AATGGAGCCT GTAATAACAT GTGATAAAAA ATTTCGTACT
CAATTTTACA TTGACTGGTG CAAAATTTCA TTGGTTGATA AAACAAAGCA AGTGTCCACC
TATCAGGAAG TGATTCGTGG AGAGGGGATT TTACCTGATG GTGGAGAATA CAAACCCCCT
TCTGATTCTT TGAAAAGCAG AGACTATTAC ACGGATTTCC TAATTACACT GGCTGTGCCC
TCGGCAGTGG CACTGGTCCT TTTTCTAATA CTTGCTTATA TCATGTGCTG CCGACGGGAA
GGCGTGGAAA AGAGAAACAT GCAAACACCA GACATCCAAC TGGTCCATCA CAGTGCTATT
CAGAAATCTA CCAAGGAGCT TCGAGACATG TCCAAGAATA GAGAGATAGC ATGGCCCCTG
TCAACGCTTC CTGTGTTCCA CCCTGTGACT GGGGAAATCA TACCTCCTTT ACACACAGAC
AACTATGATA GCACAAACAT GCCATTGATG CAAACGCAGC AGAACTTGCC ACATCAGACT
CAGATTCCCC AACAGCAGAC TACAGGTAAA TGGTATCCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62304
Accession Version XM_011516665.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1404bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product epsilon-sarcoglycan isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCAATTGC CCCGGTGGTG GGAGCTGGGA GACCCCTGTG CTTGGACGGG ACAGGGTCGG 
GGGACACGCA GGATGAGCCC CGCGACCACT GGCACATTCT TGCTGACAGT GTACAGTATT
TTCTCCAAGG TACACTCCGA TCGGAATGTA TACCCATCAG CAGGTGTCCT CTTTGTTCAT
GTTTTGGAAA GAGAATATTT TAAGGGGGAA TTTCCACCTT ACCCAAAACC TGGCGAGATT
AGTAATGATC CCATAACATT TAATACAAAT TTAATGGGTT ACCCAGACCG ACCTGGATGG
CTTCGATATA TCCAAAGGAC ACCATATAGT GATGGAGTCC TATATGGGTC CCCAACAGCT
GAAAATGTGG GGAAGCCAAC AATCATTGAG ATAACTGCCT ACAACAGGCG CACCTTTGAG
ACTGCAAGGC ATAATTTGAT AATTAATATA ATGTCTGCAG AAGACTTCCC GTTGCCATAT
CAAGCAGAAT TCTTCATTAA GAATATGAAT GTAGAAGAAA TGTTGGCCAG TGAGGTTCTT
GGAGACTTTC TTGGCGCAGT GAAAAATGTG TGGCAGCCAG AGCGCCTGAA CGCCATAAAC
ATCACATCGG CCCTAGACAG GGGTGGCAGG GTGCCACTTC CCATTAATGA CCTGAAGGAG
GGCGTTTATG TCATGGTTGG TGCAGATGTC CCGTTTTCTT CTTGTTTACG AGAAGTTGAA
AATCCACAGA ATCAATTGAG ATGTAGTCAA GAAATGGAGC CTGTAATAAC ATGTGATAAA
AAATTTCGTA CTCAATTTTA CATTGACTGG TGCAAAATTT CATTGGTTGA TAAAACAAAG
CAAGTGTCCA CCTATCAGGA AGTGATTCGT GGAGAGGGGA TTTTACCTGA TGGTGGAGAA
TACAAACCCC CTTCTGATTC TTTGAAAAGC AGAGACTATT ACACGGATTT CCTAATTACA
CTGGCTGTGC CCTCGGCAGT GGCACTGGTC CTTTTTCTAA TACTTGCTTA TATCATGTGC
TGCCGACGGG AAGGCGTGGA AAAGAGAAAC ATGCAAACAC CAGACATCCA ACTGGTCCAT
CACAGTGCTA TTCAGAAATC TACCAAGGAG CTTCGAGACA TGTCCAAGAA TAGAGAGATA
GCATGGCCCC TGTCAACGCT TCCTGTGTTC CACCCTGTGA CTGGGGAAAT CATACCTCCT
TTACACACAG ACAACTATGA TAGCACAAAC ATGCCATTGA TGCAAACGCA GCAGAACTTG
CCACATCAGA CTCAGATTCC CCAACAGCAG ACTACAGGAG ATTTTCGTTT GACAACTTTT
CAAAGATTTG AGGCAAGTAT AATGAAATTG TTCATCAGAT TTCAATTACA AGATGTTTAT
CAGAATTTTT CCCTACAATT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011514967.1
CDS108..1511
Misc Feature(1)195..1361(+)
Misc Feature(2)258..572(+)
Misc Feature(3)267..470(+)
Translation
Position Chain Variation Link
2 2 g, t dbSNP:565630781
10 10 g, t dbSNP:548813052
11 11 a, g dbSNP:529057223
24 24 c, t dbSNP:747133401
59 59 a, t dbSNP:776543055
72 72 a, g dbSNP:768305737
75 75 -, gggag dbSNP:534632849
75 75 a, g dbSNP:760407098
76 76 -, ggaga dbSNP:777001642
79 79 c, t dbSNP:775235692
86 86 c, g, t dbSNP:745436141
87 87 c, g dbSNP:773984458
88 88 a, c dbSNP:771517384
90 90 a, g dbSNP:749727444
101 101 a, g dbSNP:778226570
104 104 a, g dbSNP:756382042
109 109 c, t dbSNP:748379190
111 111 a, c dbSNP:781322593
112 112 a, c dbSNP:755191122
116 116 g, t dbSNP:751698983
118 118 c, t dbSNP:372207991
121 121 g, t dbSNP:147148588
128 128 a, g dbSNP:201378067
140 140 c, t dbSNP:753678586
144 144 -, a dbSNP:766778442
147 147 a, g dbSNP:763973436
151 151 c, g dbSNP:760330361
157 157 g, t dbSNP:373127563
162 162 a, g dbSNP:767335346
166 166 a, g dbSNP:759241679
170 170 -, g dbSNP:760824608
174 174 c, g dbSNP:774045225
175 175 g, t dbSNP:770302911
181 181 a, t dbSNP:749820152
184 184 a, g dbSNP:773531358
191 191 g, t dbSNP:770197127
200 200 c, t dbSNP:748581376
217 217 c, t dbSNP:369006527
222 222 a, t dbSNP:751226572
225 225 a, g dbSNP:766011440
232 232 c, t dbSNP:762656646
240 240 c, t dbSNP:749953335
241 241 a, c dbSNP:764776893
245 245 c, t dbSNP:116632777
246 246 a, g dbSNP:199787155
249 249 a, c, t dbSNP:760964336
250 250 a, g dbSNP:148483382
253 253 a, g dbSNP:11548284
254 254 g, t dbSNP:772230342
263 263 a, g dbSNP:746070224
276 276 -, caggtgtcc dbSNP:773475595
276 276 c, t dbSNP:778890137
278 278 c, t dbSNP:771086531
279 279 c, t dbSNP:748106020
298 298 c, g dbSNP:766866819
312 312 a, g dbSNP:754959062
316 316 a, g dbSNP:751366329
322 322 c, t dbSNP:779878703
325 325 c, t dbSNP:758030589
326 326 c, t dbSNP:750056501
341 341 c, t dbSNP:768766085
342 342 a, g dbSNP:375983969
344 344 c, g dbSNP:144527028
348 348 a, g dbSNP:758164053
381 381 a, g dbSNP:751883227
384 384 g, t dbSNP:149295657
385 385 a, g dbSNP:778474711
386 386 c, t dbSNP:756901549
396 396 c, t dbSNP:121908489
397 397 a, g dbSNP:11548285
401 401 c, t dbSNP:138616225
411 411 c, t dbSNP:121908490
412 412 a, g dbSNP:753195301
415 415 a, g dbSNP:200979763
425 425 a, g dbSNP:767782290
427 427 c, t dbSNP:759877587
431 431 a, g dbSNP:554784851
435 435 a, g dbSNP:370382282
463 463 a, c dbSNP:766764222
474 474 a, g dbSNP:369755440
476 476 c, g dbSNP:140913016
477 477 a, g dbSNP:142076876
485 485 a, c dbSNP:746942662
491 491 c, t dbSNP:138794751
494 494 c, t dbSNP:775439702
498 498 a, g dbSNP:370609227
502 502 c, t dbSNP:772108295
516 516 c, t dbSNP:557861177
517 517 a, g, t dbSNP:548306335
520 520 c, g, t dbSNP:777335847
530 530 -, act dbSNP:539327067
531 531 a, g dbSNP:769326027
535 535 a, g dbSNP:747750684
536 536 g, t dbSNP:145010266
543 543 a, c, t dbSNP:752074255
552 552 a, g dbSNP:780589840
556 556 c, t dbSNP:572107071
559 559 a, t dbSNP:750679376
563 563 c, t dbSNP:765566124
577 577 c, t dbSNP:768233445
578 578 a, g dbSNP:747531940
591 591 a, g dbSNP:780640982
594 594 g, t dbSNP:758973154
605 605 g, t dbSNP:746296901
607 607 a, c dbSNP:779127500
611 611 a, t dbSNP:757542604
612 612 a, g dbSNP:753955515
614 614 g, t dbSNP:764244520
617 617 c, t dbSNP:55853245
628 628 c, t dbSNP:752764608
640 640 a, c dbSNP:766361503
642 642 g, t dbSNP:762854508
645 645 a, c dbSNP:376936851
647 647 c, t dbSNP:199727473
657 657 c, t dbSNP:761551499
659 659 c, t dbSNP:776091871
662 662 c, t dbSNP:768286622
663 663 a, g dbSNP:746585235
686 686 a, g dbSNP:148169153
690 690 c, t dbSNP:143839858
691 691 a, g dbSNP:746331571
694 694 g, t dbSNP:121908491
698 698 c, t dbSNP:372968907
699 699 a, g, t dbSNP:749526870
704 704 a, g dbSNP:777920643
713 713 a, c, g dbSNP:148979783
716 716 a, g, t dbSNP:779674432
724 724 a, g dbSNP:727504150
740 740 a, g dbSNP:758362726
743 743 a, g dbSNP:750280111
766 766 a, t dbSNP:765095895
767 767 c, g dbSNP:369157830
770 770 c, t dbSNP:781270494
771 771 a, g dbSNP:754956483
777 777 a, g dbSNP:374149816
781 781 c, t dbSNP:549213726
796 796 c, t dbSNP:201158658
799 799 c, t dbSNP:755996611
800 800 a, g dbSNP:757206832
802 802 c, t dbSNP:200912914
816 816 c, t dbSNP:398123812
819 819 a, c, g dbSNP:200035109
823 823 c, t dbSNP:142833106
839 839 g, t dbSNP:760437933
843 843 c, t dbSNP:752351941
845 845 a, g dbSNP:767137229
870 870 a, g dbSNP:759032005
871 871 c, t dbSNP:773893939
876 876 a, c dbSNP:116105264
890 890 a, g dbSNP:763484333
894 894 c, t dbSNP:199871508
895 895 a, g, t dbSNP:375899729
906 906 a, t dbSNP:781326840
919 919 a, g dbSNP:372686312
928 928 c, t dbSNP:747116474
930 930 c, t dbSNP:780163710
943 943 c, t dbSNP:747227969
949 949 a, g dbSNP:200400681
962 962 c, t dbSNP:201234561
963 963 c, g dbSNP:775468466
971 971 c, g dbSNP:772144979
975 975 a, c, g, t dbSNP:576417569
976 976 a, g dbSNP:748001731
986 986 a, g dbSNP:781005887
987 987 a, t dbSNP:556458255
991 991 c, t dbSNP:142079200
993 993 c, t dbSNP:375828258
999 999 a, g dbSNP:757835571
1000 1000 a, g dbSNP:749932459
1002 1002 a, g dbSNP:765792098
1006 1006 a, c dbSNP:11548286
1012 1012 a, t dbSNP:762287154
1014 1014 c, t dbSNP:777023291
1016 1016 c, t dbSNP:764483578
1035 1035 a, g dbSNP:760832014
1041 1041 a, g dbSNP:775727447
1044 1044 c, t dbSNP:774701123
1046 1046 c, t dbSNP:772177078
1052 1052 a, g dbSNP:539333988
1067 1067 a, c dbSNP:774546647
1073 1073 a, t dbSNP:769888226
1074 1074 a, g dbSNP:147550814
1076 1076 a, g dbSNP:780870614
1080 1080 g, t dbSNP:754742075
1081 1081 c, t dbSNP:559353446
1082 1082 a, g dbSNP:145040065
1103 1103 c, t dbSNP:779825167
1105 1105 c, t dbSNP:758035606
1116 1116 c, t dbSNP:749985422
1132 1132 a, g dbSNP:764696852
1134 1134 c, t dbSNP:757796461
1135 1135 a, g dbSNP:755083075
1136 1136 g, t dbSNP:764383967
1140 1140 a, g dbSNP:553677309
1143 1143 a, g dbSNP:533652380
1146 1146 c, g dbSNP:756703589
1150 1150 a, g dbSNP:754309608
1162 1162 a, g dbSNP:186919286
1165 1165 -, cacca dbSNP:758213105
1168 1168 c, t dbSNP:778398331
1169 1169 a, g dbSNP:756552102
1179 1179 c, t dbSNP:139062360
1208 1208 a, t dbSNP:756672671
1213 1213 a, c dbSNP:753092622
1216 1216 a, g dbSNP:781699232
1221 1221 c, t dbSNP:121908492
1222 1222 a, g dbSNP:373904456
1228 1228 c, t dbSNP:751967746
1235 1235 a, g dbSNP:766764410
1236 1236 a, c dbSNP:763064939
1242 1242 a, g dbSNP:750556405
1246 1246 g, t dbSNP:765193255
1256 1256 c, g dbSNP:760687034
1259 1259 c, g dbSNP:775351067
1262 1262 a, g dbSNP:767308451
1263 1263 a, g dbSNP:759396645
1264 1264 c, g, t dbSNP:370800943
1265 1265 a, g dbSNP:368276611
1272 1272 c, g dbSNP:374697567
1283 1283 c, t dbSNP:769125810
1284 1284 a, g dbSNP:150797717
1285 1285 c, t dbSNP:200020922
1293 1293 g, t dbSNP:781781262
1295 1295 a, g dbSNP:755528173
1302 1302 c, t dbSNP:747427574
1303 1303 a, c, t dbSNP:17851923
1313 1313 c, t dbSNP:758680153
1324 1324 a, g dbSNP:148660601
1333 1333 c, t dbSNP:545029310
1344 1344 c, t dbSNP:147387740
1354 1354 c, t dbSNP:560154922
1355 1355 a, c, g dbSNP:759435338
1374 1374 c, t dbSNP:766199553
1382 1382 c, g dbSNP:762720842
1388 1388 c, g, t dbSNP:148126317
1392 1392 c, g dbSNP:371568101
1397 1397 a, g dbSNP:370115639
1401 1401 a, g dbSNP:776313613
1414 1414 a, g dbSNP:370584439
1435 1435 g, t dbSNP:763703803
1440 1440 g, t dbSNP:760331718
1455 1455 c, t dbSNP:530763000
1457 1457 g, t dbSNP:775056533
1473 1473 c, t dbSNP:772509228
1503 1503 c, t dbSNP:188711194
1505 1505 a, g dbSNP:551544662
1539 1539 a, g dbSNP:767828846
1556 1556 c, g dbSNP:759942828
1560 1560 a, g dbSNP:528155494
1587 1587 -, a dbSNP:764856244
1636 1636 a, c dbSNP:559397123
1653 1653 a, c dbSNP:775004588
1668 1668 a, t dbSNP:562232172

Target ORF information:

RefSeq Version XM_011516665
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516665

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCAATTGC CCCGGTGGTG GGAGCTGGGA GACCCCTGTG CTTGGACGGG ACAGGGTCGG 
GGGACACGCA GGATGAGCCC CGCGACCACT GGCACATTCT TGCTGACAGT GTACAGTATT
TTCTCCAAGG TACACTCCGA TCGGAATGTA TACCCATCAG CAGGTGTCCT CTTTGTTCAT
GTTTTGGAAA GAGAATATTT TAAGGGGGAA TTTCCACCTT ACCCAAAACC TGGCGAGATT
AGTAATGATC CCATAACATT TAATACAAAT TTAATGGGTT ACCCAGACCG ACCTGGATGG
CTTCGATATA TCCAAAGGAC ACCATATAGT GATGGAGTCC TATATGGGTC CCCAACAGCT
GAAAATGTGG GGAAGCCAAC AATCATTGAG ATAACTGCCT ACAACAGGCG CACCTTTGAG
ACTGCAAGGC ATAATTTGAT AATTAATATA ATGTCTGCAG AAGACTTCCC GTTGCCATAT
CAAGCAGAAT TCTTCATTAA GAATATGAAT GTAGAAGAAA TGTTGGCCAG TGAGGTTCTT
GGAGACTTTC TTGGCGCAGT GAAAAATGTG TGGCAGCCAG AGCGCCTGAA CGCCATAAAC
ATCACATCGG CCCTAGACAG GGGTGGCAGG GTGCCACTTC CCATTAATGA CCTGAAGGAG
GGCGTTTATG TCATGGTTGG TGCAGATGTC CCGTTTTCTT CTTGTTTACG AGAAGTTGAA
AATCCACAGA ATCAATTGAG ATGTAGTCAA GAAATGGAGC CTGTAATAAC ATGTGATAAA
AAATTTCGTA CTCAATTTTA CATTGACTGG TGCAAAATTT CATTGGTTGA TAAAACAAAG
CAAGTGTCCA CCTATCAGGA AGTGATTCGT GGAGAGGGGA TTTTACCTGA TGGTGGAGAA
TACAAACCCC CTTCTGATTC TTTGAAAAGC AGAGACTATT ACACGGATTT CCTAATTACA
CTGGCTGTGC CCTCGGCAGT GGCACTGGTC CTTTTTCTAA TACTTGCTTA TATCATGTGC
TGCCGACGGG AAGGCGTGGA AAAGAGAAAC ATGCAAACAC CAGACATCCA ACTGGTCCAT
CACAGTGCTA TTCAGAAATC TACCAAGGAG CTTCGAGACA TGTCCAAGAA TAGAGAGATA
GCATGGCCCC TGTCAACGCT TCCTGTGTTC CACCCTGTGA CTGGGGAAAT CATACCTCCT
TTACACACAG ACAACTATGA TAGCACAAAC ATGCCATTGA TGCAAACGCA GCAGAACTTG
CCACATCAGA CTCAGATTCC CCAACAGCAG ACTACAGGAG ATTTTCGTTT GACAACTTTT
CAAAGATTTG AGGCAAGTAT AATGAAATTG TTCATCAGAT TTCAATTACA AGATGTTTAT
CAGAATTTTT CCCTACAATT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41838
Accession Version XM_005250677.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1395bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product epsilon-sarcoglycan isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578814715. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCAATTGC CCCGGTGGTG GGAGCTGGGA GACCCCTGTG CTTGGACGGG ACAGGGTCGG 
GGGACACGCA GGATGAGCCC CGCGACCACT GGCACATTCT TGCTGACAGC TTTTTTATTA
AGCTGTGCTG ATGGCATAAA TGGAACAGTT AACTGGAAGA CCAGACAGGC CAACAATTTT
ATTATCAGCA GAAAAATGGC TGCTGGGATG AAAGATGTGT ACAGTATTTT CTCCAAGGTA
CACTCCGATC GGAATGTATA CCCATCAGCA GGTGTCCTCT TTGTTCATGT TTTGGAAAGA
GAATATTTTA AGGGGGAATT TCCACCTTAC CCAAAACCTG GCGAGATTAG TAATGATCCC
ATAACATTTA ATACAAATTT AATGGGTTAC CCAGACCGAC CTGGATGGCT TCGATATATC
CAAAGGACAC CATATAGTGA TGGAGTCCTA TATGGGTCCC CAACAGCTGA AAATGTGGGG
AAGCCAACAA TCATTGAGAT AACTGCCTAC AACAGGCGCA CCTTTGAGAC TGCAAGGCAT
AATTTGATAA TTAATATAAT GTCTGCAGAA GACTTCCCGT TGCCATATCA AGCAGAATTC
TTCATTAAGA ATATGAATGT AGAAGAAATG TTGGCCAGTG AGGTTCTTGG AGACTTTCTT
GGCGCAGTGA AAAATGTGTG GCAGCCAGAG CGCCTGAACG CCATAAACAT CACATCGGCC
CTAGACAGGG GTGGCAGGGT GCCACTTCCC ATTAATGACC TGAAGGAGGG CGTTTATGTC
ATGGTTGGTG CAGATGTCCC GTTTTCTTCT TGTTTACGAG AAGTTGAAAA TCCACAGAAT
CAATTGAGAT GTAGTCAAGA AATGGAGCCT GTAATAACAT GTGATAAAAA ATTTCGTACT
CAATTTTACA TTGACTGGTG CAAAATTTCA TTGGTTGATA AAACAAAGCA AGTGTCCACC
TATCAGGAAG TGATTCGTGG AGAGGGGATT TTACCTGATG GTGGAGAATA CAAACCCCCT
TCTGATTCTT TGAAAAGCAG AGACTATTAC ACGGATTTCC TAATTACACT GGCTGTGCCC
TCGGCAGTGG CACTGGTCCT TTTTCTAATA CTTGCTTATA TCATGTGCTG CCGACGGGAA
GGCGTCATCC AACTGGTCCA TCACAGTGCT ATTCAGAAAT CTACCAAGGA GCTTCGAGAC
ATGTCCAAGA ATAGAGAGAT AGCATGGCCC CTGTCAACGC TTCCTGTGTT CCACCCTGTG
ACTGGGGAAA TCATACCTCC TTTACACACA GACAACTATG ATAGCACAAA CATGCCATTG
ATGCAAACGC AGCAGAACTT GCCACATCAG ACTCAGATTC CCCAACAGCA GACTACAGGT
AAATGGTATC CCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005250734.1
CDS84..1478
Misc Feature(1)300..1418(+)
Misc Feature(2)342..656(+)
Misc Feature(3)351..554(+)
Translation
Position Chain Variation Link
35 35 a, t dbSNP:776543055
48 48 a, g dbSNP:768305737
51 51 -, gggag dbSNP:534632849
51 51 a, g dbSNP:760407098
52 52 -, ggaga dbSNP:777001642
55 55 c, t dbSNP:775235692
62 62 c, g, t dbSNP:745436141
63 63 c, g dbSNP:773984458
64 64 a, c dbSNP:771517384
66 66 a, g dbSNP:749727444
77 77 a, g dbSNP:778226570
80 80 a, g dbSNP:756382042
85 85 c, t dbSNP:748379190
87 87 a, c dbSNP:781322593
88 88 a, c dbSNP:755191122
92 92 g, t dbSNP:751698983
94 94 c, t dbSNP:372207991
97 97 g, t dbSNP:147148588
104 104 a, g dbSNP:201378067
116 116 c, t dbSNP:753678586
120 120 -, a dbSNP:766778442
123 123 a, g dbSNP:763973436
127 127 c, g dbSNP:760330361
133 133 g, t dbSNP:373127563
138 138 a, g dbSNP:767335346
142 142 a, g dbSNP:759241679
146 146 -, g dbSNP:760824608
150 150 c, g dbSNP:774045225
151 151 g, t dbSNP:770302911
157 157 a, t dbSNP:749820152
160 160 a, g dbSNP:773531358
167 167 g, t dbSNP:770197127
176 176 c, t dbSNP:748581376
211 211 c, t dbSNP:761677071
220 220 c, t dbSNP:142744182
241 241 a, c, g dbSNP:116324366
248 248 a, g dbSNP:768630572
262 262 c, t dbSNP:534807014
282 282 a, g dbSNP:565647846
301 301 c, t dbSNP:369006527
306 306 a, t dbSNP:751226572
309 309 a, g dbSNP:766011440
316 316 c, t dbSNP:762656646
324 324 c, t dbSNP:749953335
325 325 a, c dbSNP:764776893
329 329 c, t dbSNP:116632777
330 330 a, g dbSNP:199787155
333 333 a, c, t dbSNP:760964336
334 334 a, g dbSNP:148483382
337 337 a, g dbSNP:11548284
338 338 g, t dbSNP:772230342
347 347 a, g dbSNP:746070224
360 360 -, caggtgtcc dbSNP:773475595
360 360 c, t dbSNP:778890137
362 362 c, t dbSNP:771086531
363 363 c, t dbSNP:748106020
382 382 c, g dbSNP:766866819
396 396 a, g dbSNP:754959062
400 400 a, g dbSNP:751366329
406 406 c, t dbSNP:779878703
409 409 c, t dbSNP:758030589
410 410 c, t dbSNP:750056501
425 425 c, t dbSNP:768766085
426 426 a, g dbSNP:375983969
428 428 c, g dbSNP:144527028
432 432 a, g dbSNP:758164053
465 465 a, g dbSNP:751883227
468 468 g, t dbSNP:149295657
469 469 a, g dbSNP:778474711
470 470 c, t dbSNP:756901549
480 480 c, t dbSNP:121908489
481 481 a, g dbSNP:11548285
485 485 c, t dbSNP:138616225
495 495 c, t dbSNP:121908490
496 496 a, g dbSNP:753195301
499 499 a, g dbSNP:200979763
509 509 a, g dbSNP:767782290
511 511 c, t dbSNP:759877587
515 515 a, g dbSNP:554784851
519 519 a, g dbSNP:370382282
547 547 a, c dbSNP:766764222
558 558 a, g dbSNP:369755440
560 560 c, g dbSNP:140913016
561 561 a, g dbSNP:142076876
569 569 a, c dbSNP:746942662
575 575 c, t dbSNP:138794751
578 578 c, t dbSNP:775439702
582 582 a, g dbSNP:370609227
586 586 c, t dbSNP:772108295
600 600 c, t dbSNP:557861177
601 601 a, g, t dbSNP:548306335
604 604 c, g, t dbSNP:777335847
614 614 -, act dbSNP:539327067
615 615 a, g dbSNP:769326027
619 619 a, g dbSNP:747750684
620 620 g, t dbSNP:145010266
627 627 a, c, t dbSNP:752074255
636 636 a, g dbSNP:780589840
640 640 c, t dbSNP:572107071
643 643 a, t dbSNP:750679376
647 647 c, t dbSNP:765566124
661 661 c, t dbSNP:768233445
662 662 a, g dbSNP:747531940
675 675 a, g dbSNP:780640982
678 678 g, t dbSNP:758973154
689 689 g, t dbSNP:746296901
691 691 a, c dbSNP:779127500
695 695 a, t dbSNP:757542604
696 696 a, g dbSNP:753955515
698 698 g, t dbSNP:764244520
701 701 c, t dbSNP:55853245
712 712 c, t dbSNP:752764608
724 724 a, c dbSNP:766361503
726 726 g, t dbSNP:762854508
729 729 a, c dbSNP:376936851
731 731 c, t dbSNP:199727473
741 741 c, t dbSNP:761551499
743 743 c, t dbSNP:776091871
746 746 c, t dbSNP:768286622
747 747 a, g dbSNP:746585235
770 770 a, g dbSNP:148169153
774 774 c, t dbSNP:143839858
775 775 a, g dbSNP:746331571
778 778 g, t dbSNP:121908491
782 782 c, t dbSNP:372968907
783 783 a, g, t dbSNP:749526870
788 788 a, g dbSNP:777920643
797 797 a, c, g dbSNP:148979783
800 800 a, g, t dbSNP:779674432
808 808 a, g dbSNP:727504150
824 824 a, g dbSNP:758362726
827 827 a, g dbSNP:750280111
850 850 a, t dbSNP:765095895
851 851 c, g dbSNP:369157830
854 854 c, t dbSNP:781270494
855 855 a, g dbSNP:754956483
861 861 a, g dbSNP:374149816
865 865 c, t dbSNP:549213726
880 880 c, t dbSNP:201158658
883 883 c, t dbSNP:755996611
884 884 a, g dbSNP:757206832
886 886 c, t dbSNP:200912914
900 900 c, t dbSNP:398123812
903 903 a, c, g dbSNP:200035109
907 907 c, t dbSNP:142833106
923 923 g, t dbSNP:760437933
927 927 c, t dbSNP:752351941
929 929 a, g dbSNP:767137229
954 954 a, g dbSNP:759032005
955 955 c, t dbSNP:773893939
960 960 a, c dbSNP:116105264
974 974 a, g dbSNP:763484333
978 978 c, t dbSNP:199871508
979 979 a, g, t dbSNP:375899729
990 990 a, t dbSNP:781326840
1003 1003 a, g dbSNP:372686312
1012 1012 c, t dbSNP:747116474
1014 1014 c, t dbSNP:780163710
1027 1027 c, t dbSNP:747227969
1033 1033 a, g dbSNP:200400681
1046 1046 c, t dbSNP:201234561
1047 1047 c, g dbSNP:775468466
1055 1055 c, g dbSNP:772144979
1059 1059 a, c, g, t dbSNP:576417569
1060 1060 a, g dbSNP:748001731
1070 1070 a, g dbSNP:781005887
1071 1071 a, t dbSNP:556458255
1075 1075 c, t dbSNP:142079200
1077 1077 c, t dbSNP:375828258
1083 1083 a, g dbSNP:757835571
1084 1084 a, g dbSNP:749932459
1086 1086 a, g dbSNP:765792098
1090 1090 a, c dbSNP:11548286
1096 1096 a, t dbSNP:762287154
1098 1098 c, t dbSNP:777023291
1100 1100 c, t dbSNP:764483578
1119 1119 a, g dbSNP:760832014
1125 1125 a, g dbSNP:775727447
1128 1128 c, t dbSNP:774701123
1130 1130 c, t dbSNP:772177078
1136 1136 a, g dbSNP:539333988
1151 1151 a, c dbSNP:774546647
1157 1157 a, t dbSNP:769888226
1158 1158 a, g dbSNP:147550814
1160 1160 a, g dbSNP:780870614
1164 1164 g, t dbSNP:754742075
1165 1165 c, t dbSNP:559353446
1166 1166 a, g dbSNP:145040065
1187 1187 c, t dbSNP:779825167
1189 1189 c, t dbSNP:758035606
1200 1200 c, t dbSNP:749985422
1216 1216 a, g dbSNP:764696852
1218 1218 c, t dbSNP:757796461
1219 1219 a, g dbSNP:755083075
1220 1220 g, t dbSNP:764383967
1224 1224 a, g dbSNP:553677309
1227 1227 a, g dbSNP:533652380
1236 1236 c, t dbSNP:139062360
1265 1265 a, t dbSNP:756672671
1270 1270 a, c dbSNP:753092622
1273 1273 a, g dbSNP:781699232
1278 1278 c, t dbSNP:121908492
1279 1279 a, g dbSNP:373904456
1285 1285 c, t dbSNP:751967746
1292 1292 a, g dbSNP:766764410
1293 1293 a, c dbSNP:763064939
1299 1299 a, g dbSNP:750556405
1303 1303 g, t dbSNP:765193255
1313 1313 c, g dbSNP:760687034
1316 1316 c, g dbSNP:775351067
1319 1319 a, g dbSNP:767308451
1320 1320 a, g dbSNP:759396645
1321 1321 c, g, t dbSNP:370800943
1322 1322 a, g dbSNP:368276611
1329 1329 c, g dbSNP:374697567
1340 1340 c, t dbSNP:769125810
1341 1341 a, g dbSNP:150797717
1342 1342 c, t dbSNP:200020922
1350 1350 g, t dbSNP:781781262
1352 1352 a, g dbSNP:755528173
1359 1359 c, t dbSNP:747427574
1360 1360 a, c, t dbSNP:17851923
1370 1370 c, t dbSNP:758680153
1381 1381 a, g dbSNP:148660601
1390 1390 c, t dbSNP:545029310
1401 1401 c, t dbSNP:147387740
1411 1411 c, t dbSNP:560154922
1412 1412 a, c, g dbSNP:759435338
1431 1431 c, t dbSNP:766199553
1439 1439 c, g dbSNP:762720842
1445 1445 c, g, t dbSNP:148126317
1449 1449 c, g dbSNP:371568101
1454 1454 a, g dbSNP:370115639
1458 1458 a, g dbSNP:776313613
1462 1462 a, g dbSNP:750326561
1474 1474 c, t dbSNP:778866625
1490 1490 g, t dbSNP:757047752
1508 1508 c, t dbSNP:753684985
1515 1515 a, c dbSNP:760109715
1579 1579 a, g dbSNP:193172306
1633 1633 c, g dbSNP:527929196
1650 1650 c, t dbSNP:112954947
1674 1674 c, t dbSNP:558194543
1682 1682 a, g dbSNP:542318820
1688 1688 c, t dbSNP:188526246
1740 1740 -, tta dbSNP:532244072
1750 1750 c, t dbSNP:11984148

Target ORF information:

RefSeq Version XM_005250677
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005250677

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCAATTGC CCCGGTGGTG GGAGCTGGGA GACCCCTGTG CTTGGACGGG ACAGGGTCGG 
GGGACACGCA GGATGAGCCC CGCGACCACT GGCACATTCT TGCTGACAGC TTTTTTATTA
AGCTGTGCTG ATGGCATAAA TGGAACAGTT AACTGGAAGA CCAGACAGGC CAACAATTTT
ATTATCAGCA GAAAAATGGC TGCTGGGATG AAAGATGTGT ACAGTATTTT CTCCAAGGTA
CACTCCGATC GGAATGTATA CCCATCAGCA GGTGTCCTCT TTGTTCATGT TTTGGAAAGA
GAATATTTTA AGGGGGAATT TCCACCTTAC CCAAAACCTG GCGAGATTAG TAATGATCCC
ATAACATTTA ATACAAATTT AATGGGTTAC CCAGACCGAC CTGGATGGCT TCGATATATC
CAAAGGACAC CATATAGTGA TGGAGTCCTA TATGGGTCCC CAACAGCTGA AAATGTGGGG
AAGCCAACAA TCATTGAGAT AACTGCCTAC AACAGGCGCA CCTTTGAGAC TGCAAGGCAT
AATTTGATAA TTAATATAAT GTCTGCAGAA GACTTCCCGT TGCCATATCA AGCAGAATTC
TTCATTAAGA ATATGAATGT AGAAGAAATG TTGGCCAGTG AGGTTCTTGG AGACTTTCTT
GGCGCAGTGA AAAATGTGTG GCAGCCAGAG CGCCTGAACG CCATAAACAT CACATCGGCC
CTAGACAGGG GTGGCAGGGT GCCACTTCCC ATTAATGACC TGAAGGAGGG CGTTTATGTC
ATGGTTGGTG CAGATGTCCC GTTTTCTTCT TGTTTACGAG AAGTTGAAAA TCCACAGAAT
CAATTGAGAT GTAGTCAAGA AATGGAGCCT GTAATAACAT GTGATAAAAA ATTTCGTACT
CAATTTTACA TTGACTGGTG CAAAATTTCA TTGGTTGATA AAACAAAGCA AGTGTCCACC
TATCAGGAAG TGATTCGTGG AGAGGGGATT TTACCTGATG GTGGAGAATA CAAACCCCCT
TCTGATTCTT TGAAAAGCAG AGACTATTAC ACGGATTTCC TAATTACACT GGCTGTGCCC
TCGGCAGTGG CACTGGTCCT TTTTCTAATA CTTGCTTATA TCATGTGCTG CCGACGGGAA
GGCGTCATCC AACTGGTCCA TCACAGTGCT ATTCAGAAAT CTACCAAGGA GCTTCGAGAC
ATGTCCAAGA ATAGAGAGAT AGCATGGCCC CTGTCAACGC TTCCTGTGTT CCACCCTGTG
ACTGGGGAAA TCATACCTCC TTTACACACA GACAACTATG ATAGCACAAA CATGCCATTG
ATGCAAACGC AGCAGAACTT GCCACATCAG ACTCAGATTC CCCAACAGCA GACTACAGGT
AAATGGTATC CCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62305
Accession Version XM_011516666.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1377bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product epsilon-sarcoglycan isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCAATTGC CCCGGTGGTG GGAGCTGGGA GACCCCTGTG CTTGGACGGG ACAGGGTCGG 
GGGACACGCA GGATGAGCCC CGCGACCACT GGCACATTCT TGCTGACAGT GTACAGTATT
TTCTCCAAGG TACACTCCGA TCGGAATGTA TACCCATCAG CAGGTGTCCT CTTTGTTCAT
GTTTTGGAAA GAGAATATTT TAAGGGGGAA TTTCCACCTT ACCCAAAACC TGGCGAGATT
AGTAATGATC CCATAACATT TAATACAAAT TTAATGGGTT ACCCAGACCG ACCTGGATGG
CTTCGATATA TCCAAAGGAC ACCATATAGT GATGGAGTCC TATATGGGTC CCCAACAGCT
GAAAATGTGG GGAAGCCAAC AATCATTGAG ATAACTGCCT ACAACAGGCG CACCTTTGAG
ACTGCAAGGC ATAATTTGAT AATTAATATA ATGTCTGCAG AAGACTTCCC GTTGCCATAT
CAAGCAGAAT TCTTCATTAA GAATATGAAT GTAGAAGAAA TGTTGGCCAG TGAGGTTCTT
GGAGACTTTC TTGGCGCAGT GAAAAATGTG TGGCAGCCAG AGCGCCTGAA CGCCATAAAC
ATCACATCGG CCCTAGACAG GGGTGGCAGG GTGCCACTTC CCATTAATGA CCTGAAGGAG
GGCGTTTATG TCATGGTTGG TGCAGATGTC CCGTTTTCTT CTTGTTTACG AGAAGTTGAA
AATCCACAGA ATCAATTGAG ATGTAGTCAA GAAATGGAGC CTGTAATAAC ATGTGATAAA
AAATTTCGTA CTCAATTTTA CATTGACTGG TGCAAAATTT CATTGGTTGA TAAAACAAAG
CAAGTGTCCA CCTATCAGGA AGTGATTCGT GGAGAGGGGA TTTTACCTGA TGGTGGAGAA
TACAAACCCC CTTCTGATTC TTTGAAAAGC AGAGACTATT ACACGGATTT CCTAATTACA
CTGGCTGTGC CCTCGGCAGT GGCACTGGTC CTTTTTCTAA TACTTGCTTA TATCATGTGC
TGCCGACGGG AAGGCGTCAT CCAACTGGTC CATCACAGTG CTATTCAGAA ATCTACCAAG
GAGCTTCGAG ACATGTCCAA GAATAGAGAG ATAGCATGGC CCCTGTCAAC GCTTCCTGTG
TTCCACCCTG TGACTGGGGA AATCATACCT CCTTTACACA CAGACAACTA TGATAGCACA
AACATGCCAT TGATGCAAAC GCAGCAGAAC TTGCCACATC AGACTCAGAT TCCCCAACAG
CAGACTACAG GAGATTTTCG TTTGACAACT TTTCAAAGAT TTGAGGCAAG TATAATGAAA
TTGTTCATCA GATTTCAATT ACAAGATGTT TATCAGAATT TTTCCCTACA ATTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011514968.1
CDS108..1484
Misc Feature(1)195..1334(+)
Misc Feature(2)258..572(+)
Misc Feature(3)267..470(+)
Translation
Position Chain Variation Link
2 2 g, t dbSNP:565630781
10 10 g, t dbSNP:548813052
11 11 a, g dbSNP:529057223
24 24 c, t dbSNP:747133401
59 59 a, t dbSNP:776543055
72 72 a, g dbSNP:768305737
75 75 -, gggag dbSNP:534632849
75 75 a, g dbSNP:760407098
76 76 -, ggaga dbSNP:777001642
79 79 c, t dbSNP:775235692
86 86 c, g, t dbSNP:745436141
87 87 c, g dbSNP:773984458
88 88 a, c dbSNP:771517384
90 90 a, g dbSNP:749727444
101 101 a, g dbSNP:778226570
104 104 a, g dbSNP:756382042
109 109 c, t dbSNP:748379190
111 111 a, c dbSNP:781322593
112 112 a, c dbSNP:755191122
116 116 g, t dbSNP:751698983
118 118 c, t dbSNP:372207991
121 121 g, t dbSNP:147148588
128 128 a, g dbSNP:201378067
140 140 c, t dbSNP:753678586
144 144 -, a dbSNP:766778442
147 147 a, g dbSNP:763973436
151 151 c, g dbSNP:760330361
157 157 g, t dbSNP:373127563
162 162 a, g dbSNP:767335346
166 166 a, g dbSNP:759241679
170 170 -, g dbSNP:760824608
174 174 c, g dbSNP:774045225
175 175 g, t dbSNP:770302911
181 181 a, t dbSNP:749820152
184 184 a, g dbSNP:773531358
191 191 g, t dbSNP:770197127
200 200 c, t dbSNP:748581376
217 217 c, t dbSNP:369006527
222 222 a, t dbSNP:751226572
225 225 a, g dbSNP:766011440
232 232 c, t dbSNP:762656646
240 240 c, t dbSNP:749953335
241 241 a, c dbSNP:764776893
245 245 c, t dbSNP:116632777
246 246 a, g dbSNP:199787155
249 249 a, c, t dbSNP:760964336
250 250 a, g dbSNP:148483382
253 253 a, g dbSNP:11548284
254 254 g, t dbSNP:772230342
263 263 a, g dbSNP:746070224
276 276 -, caggtgtcc dbSNP:773475595
276 276 c, t dbSNP:778890137
278 278 c, t dbSNP:771086531
279 279 c, t dbSNP:748106020
298 298 c, g dbSNP:766866819
312 312 a, g dbSNP:754959062
316 316 a, g dbSNP:751366329
322 322 c, t dbSNP:779878703
325 325 c, t dbSNP:758030589
326 326 c, t dbSNP:750056501
341 341 c, t dbSNP:768766085
342 342 a, g dbSNP:375983969
344 344 c, g dbSNP:144527028
348 348 a, g dbSNP:758164053
381 381 a, g dbSNP:751883227
384 384 g, t dbSNP:149295657
385 385 a, g dbSNP:778474711
386 386 c, t dbSNP:756901549
396 396 c, t dbSNP:121908489
397 397 a, g dbSNP:11548285
401 401 c, t dbSNP:138616225
411 411 c, t dbSNP:121908490
412 412 a, g dbSNP:753195301
415 415 a, g dbSNP:200979763
425 425 a, g dbSNP:767782290
427 427 c, t dbSNP:759877587
431 431 a, g dbSNP:554784851
435 435 a, g dbSNP:370382282
463 463 a, c dbSNP:766764222
474 474 a, g dbSNP:369755440
476 476 c, g dbSNP:140913016
477 477 a, g dbSNP:142076876
485 485 a, c dbSNP:746942662
491 491 c, t dbSNP:138794751
494 494 c, t dbSNP:775439702
498 498 a, g dbSNP:370609227
502 502 c, t dbSNP:772108295
516 516 c, t dbSNP:557861177
517 517 a, g, t dbSNP:548306335
520 520 c, g, t dbSNP:777335847
530 530 -, act dbSNP:539327067
531 531 a, g dbSNP:769326027
535 535 a, g dbSNP:747750684
536 536 g, t dbSNP:145010266
543 543 a, c, t dbSNP:752074255
552 552 a, g dbSNP:780589840
556 556 c, t dbSNP:572107071
559 559 a, t dbSNP:750679376
563 563 c, t dbSNP:765566124
577 577 c, t dbSNP:768233445
578 578 a, g dbSNP:747531940
591 591 a, g dbSNP:780640982
594 594 g, t dbSNP:758973154
605 605 g, t dbSNP:746296901
607 607 a, c dbSNP:779127500
611 611 a, t dbSNP:757542604
612 612 a, g dbSNP:753955515
614 614 g, t dbSNP:764244520
617 617 c, t dbSNP:55853245
628 628 c, t dbSNP:752764608
640 640 a, c dbSNP:766361503
642 642 g, t dbSNP:762854508
645 645 a, c dbSNP:376936851
647 647 c, t dbSNP:199727473
657 657 c, t dbSNP:761551499
659 659 c, t dbSNP:776091871
662 662 c, t dbSNP:768286622
663 663 a, g dbSNP:746585235
686 686 a, g dbSNP:148169153
690 690 c, t dbSNP:143839858
691 691 a, g dbSNP:746331571
694 694 g, t dbSNP:121908491
698 698 c, t dbSNP:372968907
699 699 a, g, t dbSNP:749526870
704 704 a, g dbSNP:777920643
713 713 a, c, g dbSNP:148979783
716 716 a, g, t dbSNP:779674432
724 724 a, g dbSNP:727504150
740 740 a, g dbSNP:758362726
743 743 a, g dbSNP:750280111
766 766 a, t dbSNP:765095895
767 767 c, g dbSNP:369157830
770 770 c, t dbSNP:781270494
771 771 a, g dbSNP:754956483
777 777 a, g dbSNP:374149816
781 781 c, t dbSNP:549213726
796 796 c, t dbSNP:201158658
799 799 c, t dbSNP:755996611
800 800 a, g dbSNP:757206832
802 802 c, t dbSNP:200912914
816 816 c, t dbSNP:398123812
819 819 a, c, g dbSNP:200035109
823 823 c, t dbSNP:142833106
839 839 g, t dbSNP:760437933
843 843 c, t dbSNP:752351941
845 845 a, g dbSNP:767137229
870 870 a, g dbSNP:759032005
871 871 c, t dbSNP:773893939
876 876 a, c dbSNP:116105264
890 890 a, g dbSNP:763484333
894 894 c, t dbSNP:199871508
895 895 a, g, t dbSNP:375899729
906 906 a, t dbSNP:781326840
919 919 a, g dbSNP:372686312
928 928 c, t dbSNP:747116474
930 930 c, t dbSNP:780163710
943 943 c, t dbSNP:747227969
949 949 a, g dbSNP:200400681
962 962 c, t dbSNP:201234561
963 963 c, g dbSNP:775468466
971 971 c, g dbSNP:772144979
975 975 a, c, g, t dbSNP:576417569
976 976 a, g dbSNP:748001731
986 986 a, g dbSNP:781005887
987 987 a, t dbSNP:556458255
991 991 c, t dbSNP:142079200
993 993 c, t dbSNP:375828258
999 999 a, g dbSNP:757835571
1000 1000 a, g dbSNP:749932459
1002 1002 a, g dbSNP:765792098
1006 1006 a, c dbSNP:11548286
1012 1012 a, t dbSNP:762287154
1014 1014 c, t dbSNP:777023291
1016 1016 c, t dbSNP:764483578
1035 1035 a, g dbSNP:760832014
1041 1041 a, g dbSNP:775727447
1044 1044 c, t dbSNP:774701123
1046 1046 c, t dbSNP:772177078
1052 1052 a, g dbSNP:539333988
1067 1067 a, c dbSNP:774546647
1073 1073 a, t dbSNP:769888226
1074 1074 a, g dbSNP:147550814
1076 1076 a, g dbSNP:780870614
1080 1080 g, t dbSNP:754742075
1081 1081 c, t dbSNP:559353446
1082 1082 a, g dbSNP:145040065
1103 1103 c, t dbSNP:779825167
1105 1105 c, t dbSNP:758035606
1116 1116 c, t dbSNP:749985422
1132 1132 a, g dbSNP:764696852
1134 1134 c, t dbSNP:757796461
1135 1135 a, g dbSNP:755083075
1136 1136 g, t dbSNP:764383967
1140 1140 a, g dbSNP:553677309
1143 1143 a, g dbSNP:533652380
1152 1152 c, t dbSNP:139062360
1181 1181 a, t dbSNP:756672671
1186 1186 a, c dbSNP:753092622
1189 1189 a, g dbSNP:781699232
1194 1194 c, t dbSNP:121908492
1195 1195 a, g dbSNP:373904456
1201 1201 c, t dbSNP:751967746
1208 1208 a, g dbSNP:766764410
1209 1209 a, c dbSNP:763064939
1215 1215 a, g dbSNP:750556405
1219 1219 g, t dbSNP:765193255
1229 1229 c, g dbSNP:760687034
1232 1232 c, g dbSNP:775351067
1235 1235 a, g dbSNP:767308451
1236 1236 a, g dbSNP:759396645
1237 1237 c, g, t dbSNP:370800943
1238 1238 a, g dbSNP:368276611
1245 1245 c, g dbSNP:374697567
1256 1256 c, t dbSNP:769125810
1257 1257 a, g dbSNP:150797717
1258 1258 c, t dbSNP:200020922
1266 1266 g, t dbSNP:781781262
1268 1268 a, g dbSNP:755528173
1275 1275 c, t dbSNP:747427574
1276 1276 a, c, t dbSNP:17851923
1286 1286 c, t dbSNP:758680153
1297 1297 a, g dbSNP:148660601
1306 1306 c, t dbSNP:545029310
1317 1317 c, t dbSNP:147387740
1327 1327 c, t dbSNP:560154922
1328 1328 a, c, g dbSNP:759435338
1347 1347 c, t dbSNP:766199553
1355 1355 c, g dbSNP:762720842
1361 1361 c, g, t dbSNP:148126317
1365 1365 c, g dbSNP:371568101
1370 1370 a, g dbSNP:370115639
1374 1374 a, g dbSNP:776313613
1387 1387 a, g dbSNP:370584439
1408 1408 g, t dbSNP:763703803
1413 1413 g, t dbSNP:760331718
1428 1428 c, t dbSNP:530763000
1430 1430 g, t dbSNP:775056533
1446 1446 c, t dbSNP:772509228
1476 1476 c, t dbSNP:188711194
1478 1478 a, g dbSNP:551544662
1512 1512 a, g dbSNP:767828846
1529 1529 c, g dbSNP:759942828
1533 1533 a, g dbSNP:528155494
1560 1560 -, a dbSNP:764856244
1609 1609 a, c dbSNP:559397123
1626 1626 a, c dbSNP:775004588
1641 1641 a, t dbSNP:562232172
1692 1692 a, t dbSNP:542582678
1764 1764 a, t dbSNP:149413332
1899 1899 c, g dbSNP:564045029
1900 1900 a, g dbSNP:184591117
1914 1914 a, g dbSNP:577854696
1916 1916 a, g dbSNP:550409736
1927 1927 a, g dbSNP:181929444
1957 1957 c, g dbSNP:207468289
2004 2004 a, c dbSNP:138932929
2011 2011 g, t dbSNP:572179315
2048 2048 a, c dbSNP:756204577
2075 2075 a, c dbSNP:117713497
2085 2085 a, g dbSNP:10224050
2126 2126 c, t dbSNP:368212965
2135 2135 a, g dbSNP:761432661
2180 2180 g, t dbSNP:569930848
2187 2187 c, t dbSNP:189873533
2205 2205 a, g dbSNP:374795955
2221 2221 a, g dbSNP:536937536
2227 2227 a, c dbSNP:571477191
2230 2230 -, ac dbSNP:34311900
2233 2233 -, ca dbSNP:200947714
2272 2272 -, tagt dbSNP:570295132
2392 2392 c, t dbSNP:1851422
2398 2398 c, t dbSNP:528220025
2483 2483 c, t dbSNP:11770181
2484 2484 a, g dbSNP:185746195
2518 2518 -, a dbSNP:397945128
2523 2523 -, a dbSNP:58533148
2524 2524 c, t dbSNP:528928324
2565 2565 c, t dbSNP:545463863
2567 2567 c, g dbSNP:746695033
2574 2574 a, g dbSNP:779747909
2579 2579 a, g dbSNP:527307225
2603 2603 a, g dbSNP:192622243
2608 2608 g, t dbSNP:146910891
2646 2646 c, t dbSNP:11770125
2666 2666 c, t dbSNP:11770122
2673 2673 a, g dbSNP:187795375
2688 2688 -, a dbSNP:201939274
2688 2688 a, g dbSNP:183955857
2711 2711 a, g dbSNP:771766275
2754 2754 c, t dbSNP:541191572
2769 2769 c, t dbSNP:565793555
2780 2780 a, g dbSNP:751940135
2785 2785 a, g dbSNP:537403420
2813 2813 c, t dbSNP:73425436
2841 2841 a, t dbSNP:745601660
2856 2856 a, g dbSNP:555856232
2862 2862 a, g dbSNP:541956695
2902 2902 c, g dbSNP:547583586
2955 2955 g, t dbSNP:191381456
2972 2972 c, t dbSNP:188293027
2974 2974 c, t dbSNP:778842632
2983 2983 a, t dbSNP:538851719
3026 3026 a, g dbSNP:537346506
3053 3053 c, t dbSNP:753743424
3057 3057 c, t dbSNP:114647036
3100 3100 g, t dbSNP:13222972

Target ORF information:

RefSeq Version XM_011516666
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens sarcoglycan, epsilon (SGCE), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516666

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCAATTGC CCCGGTGGTG GGAGCTGGGA GACCCCTGTG CTTGGACGGG ACAGGGTCGG 
GGGACACGCA GGATGAGCCC CGCGACCACT GGCACATTCT TGCTGACAGT GTACAGTATT
TTCTCCAAGG TACACTCCGA TCGGAATGTA TACCCATCAG CAGGTGTCCT CTTTGTTCAT
GTTTTGGAAA GAGAATATTT TAAGGGGGAA TTTCCACCTT ACCCAAAACC TGGCGAGATT
AGTAATGATC CCATAACATT TAATACAAAT TTAATGGGTT ACCCAGACCG ACCTGGATGG
CTTCGATATA TCCAAAGGAC ACCATATAGT GATGGAGTCC TATATGGGTC CCCAACAGCT
GAAAATGTGG GGAAGCCAAC AATCATTGAG ATAACTGCCT ACAACAGGCG CACCTTTGAG
ACTGCAAGGC ATAATTTGAT AATTAATATA ATGTCTGCAG AAGACTTCCC GTTGCCATAT
CAAGCAGAAT TCTTCATTAA GAATATGAAT GTAGAAGAAA TGTTGGCCAG TGAGGTTCTT
GGAGACTTTC TTGGCGCAGT GAAAAATGTG TGGCAGCCAG AGCGCCTGAA CGCCATAAAC
ATCACATCGG CCCTAGACAG GGGTGGCAGG GTGCCACTTC CCATTAATGA CCTGAAGGAG
GGCGTTTATG TCATGGTTGG TGCAGATGTC CCGTTTTCTT CTTGTTTACG AGAAGTTGAA
AATCCACAGA ATCAATTGAG ATGTAGTCAA GAAATGGAGC CTGTAATAAC ATGTGATAAA
AAATTTCGTA CTCAATTTTA CATTGACTGG TGCAAAATTT CATTGGTTGA TAAAACAAAG
CAAGTGTCCA CCTATCAGGA AGTGATTCGT GGAGAGGGGA TTTTACCTGA TGGTGGAGAA
TACAAACCCC CTTCTGATTC TTTGAAAAGC AGAGACTATT ACACGGATTT CCTAATTACA
CTGGCTGTGC CCTCGGCAGT GGCACTGGTC CTTTTTCTAA TACTTGCTTA TATCATGTGC
TGCCGACGGG AAGGCGTCAT CCAACTGGTC CATCACAGTG CTATTCAGAA ATCTACCAAG
GAGCTTCGAG ACATGTCCAA GAATAGAGAG ATAGCATGGC CCCTGTCAAC GCTTCCTGTG
TTCCACCCTG TGACTGGGGA AATCATACCT CCTTTACACA CAGACAACTA TGATAGCACA
AACATGCCAT TGATGCAAAC GCAGCAGAAC TTGCCACATC AGACTCAGAT TCCCCAACAG
CAGACTACAG GAGATTTTCG TTTGACAACT TTTCAAAGAT TTGAGGCAAG TATAATGAAA
TTGTTCATCA GATTTCAATT ACAAGATGTT TATCAGAATT TTTCCCTACA ATTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62306
Accession Version XM_011516667.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1317bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product epsilon-sarcoglycan isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCTGCTG GGATGAAAGA TGTGTACAGT ATTTTCTCCA AGGTACACTC CGATCGGAAT 
GTATACCCAT CAGCAGGTGT CCTCTTTGTT CATGTTTTGG AAAGAGAATA TTTTAAGGGG
GAATTTCCAC CTTACCCAAA ACCTGGCGAG ATTAGTAATG ATCCCATAAC ATTTAATACA
AATTTAATGG GTTACCCAGA CCGACCTGGA TGGCTTCGAT ATATCCAAAG GACACCATAT
AGTGATGGAG TCCTATATGG GTCCCCAACA GCTGAAAATG TGGGGAAGCC AACAATCATT
GAGATAACTG CCTACAACAG GCGCACCTTT GAGACTGCAA GGCATAATTT GATAATTAAT
ATAATGTCTG CAGAAGACTT CCCGTTGCCA TATCAAGCAG AATTCTTCAT TAAGAATATG
AATGTAGAAG AAATGTTGGC CAGTGAGGTT CTTGGAGACT TTCTTGGCGC AGTGAAAAAT
GTGTGGCAGC CAGAGCGCCT GAACGCCATA AACATCACAT CGGCCCTAGA CAGGGGTGGC
AGGGTGCCAC TTCCCATTAA TGACCTGAAG GAGGGCGTTT ATGTCATGGT TGGTGCAGAT
GTCCCGTTTT CTTCTTGTTT ACGAGAAGTT GAAAATCCAC AGAATCAATT GAGATGTAGT
CAAGAAATGG AGCCTGTAAT AACATGTGAT AAAAAATTTC GTACTCAATT TTACATTGAC
TGGTGCAAAA TTTCATTGGT TGATAAAACA AAGCAAGTGT CCACCTATCA GGAAGTGATT
CGTGGAGAGG GGATTTTACC TGATGGTGGA GAATACAAAC CCCCTTCTGA TTCTTTGAAA
AGCAGAGACT ATTACACGGA TTTCCTAATT ACACTGGCTG TGCCCTCGGC AGTGGCACTG
GTCCTTTTTC TAATACTTGC TTATATCATG TGCTGCCGAC GGGAAGGCGT GGAAAAGAGA
AACATGCAAA CACCAGACAT CCAACTGGTC CATCACAGTG CTATTCAGAA ATCTACCAAG
GAGCTTCGAG ACATGTCCAA GAATAGAGAG ATAGCATGGC CCCTGTCAAC GCTTCCTGTG
TTCCACCCTG TGACTGGGGA AATCATACCT CCTTTACACA CAGACAACTA TGATAGCACA
AACATGCCAT TGATGCAAAC GCAGCAGAAC TTGCCACATC AGACTCAGAT TCCCCAACAG
CAGACTACAG GAGATTTTCG TTTGACAACT TTTCAAAGAT TTGAGGCAAG TATAATGAAA
TTGTTCATCA GATTTCAATT ACAAGATGTT TATCAGAATT TTTCCCTACA ATTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011514969.1
CDS288..1604
Misc Feature(1)309..1454(+)
Misc Feature(2)351..665(+)
Misc Feature(3)360..563(+)
Translation
Position Chain Variation Link
2 2 c, t dbSNP:775235692
9 9 c, g, t dbSNP:745436141
10 10 c, g dbSNP:773984458
11 11 a, c dbSNP:771517384
13 13 a, g dbSNP:749727444
24 24 a, g dbSNP:778226570
27 27 a, g dbSNP:756382042
32 32 c, t dbSNP:748379190
34 34 a, c dbSNP:781322593
35 35 a, c dbSNP:755191122
39 39 g, t dbSNP:751698983
41 41 c, t dbSNP:372207991
44 44 g, t dbSNP:147148588
51 51 a, g dbSNP:201378067
63 63 c, t dbSNP:753678586
67 67 -, a dbSNP:766778442
70 70 a, g dbSNP:763973436
74 74 c, g dbSNP:760330361
80 80 g, t dbSNP:373127563
85 85 a, g dbSNP:767335346
89 89 a, g dbSNP:759241679
93 93 -, g dbSNP:760824608
97 97 c, g dbSNP:774045225
98 98 g, t dbSNP:770302911
104 104 a, t dbSNP:749820152
107 107 a, g dbSNP:773531358
114 114 g, t dbSNP:770197127
123 123 c, t dbSNP:748581376
152 152 a, g dbSNP:767388763
154 154 a, g dbSNP:561858113
160 160 c, t dbSNP:189630116
172 172 c, t dbSNP:576315606
220 220 c, t dbSNP:761677071
229 229 c, t dbSNP:142744182
250 250 a, c, g dbSNP:116324366
257 257 a, g dbSNP:768630572
271 271 c, t dbSNP:534807014
291 291 a, g dbSNP:565647846
310 310 c, t dbSNP:369006527
315 315 a, t dbSNP:751226572
318 318 a, g dbSNP:766011440
325 325 c, t dbSNP:762656646
333 333 c, t dbSNP:749953335
334 334 a, c dbSNP:764776893
338 338 c, t dbSNP:116632777
339 339 a, g dbSNP:199787155
342 342 a, c, t dbSNP:760964336
343 343 a, g dbSNP:148483382
346 346 a, g dbSNP:11548284
347 347 g, t dbSNP:772230342
356 356 a, g dbSNP:746070224
369 369 -, caggtgtcc dbSNP:773475595
369 369 c, t dbSNP:778890137
371 371 c, t dbSNP:771086531
372 372 c, t dbSNP:748106020
391 391 c, g dbSNP:766866819
405 405 a, g dbSNP:754959062
409 409 a, g dbSNP:751366329
415 415 c, t dbSNP:779878703
418 418 c, t dbSNP:758030589
419 419 c, t dbSNP:750056501
434 434 c, t dbSNP:768766085
435 435 a, g dbSNP:375983969
437 437 c, g dbSNP:144527028
441 441 a, g dbSNP:758164053
474 474 a, g dbSNP:751883227
477 477 g, t dbSNP:149295657
478 478 a, g dbSNP:778474711
479 479 c, t dbSNP:756901549
489 489 c, t dbSNP:121908489
490 490 a, g dbSNP:11548285
494 494 c, t dbSNP:138616225
504 504 c, t dbSNP:121908490
505 505 a, g dbSNP:753195301
508 508 a, g dbSNP:200979763
518 518 a, g dbSNP:767782290
520 520 c, t dbSNP:759877587
524 524 a, g dbSNP:554784851
528 528 a, g dbSNP:370382282
556 556 a, c dbSNP:766764222
567 567 a, g dbSNP:369755440
569 569 c, g dbSNP:140913016
570 570 a, g dbSNP:142076876
578 578 a, c dbSNP:746942662
584 584 c, t dbSNP:138794751
587 587 c, t dbSNP:775439702
591 591 a, g dbSNP:370609227
595 595 c, t dbSNP:772108295
609 609 c, t dbSNP:557861177
610 610 a, g, t dbSNP:548306335
613 613 c, g, t dbSNP:777335847
623 623 -, act dbSNP:539327067
624 624 a, g dbSNP:769326027
628 628 a, g dbSNP:747750684
629 629 g, t dbSNP:145010266
636 636 a, c, t dbSNP:752074255
645 645 a, g dbSNP:780589840
649 649 c, t dbSNP:572107071
652 652 a, t dbSNP:750679376
656 656 c, t dbSNP:765566124
670 670 c, t dbSNP:768233445
671 671 a, g dbSNP:747531940
684 684 a, g dbSNP:780640982
687 687 g, t dbSNP:758973154
698 698 g, t dbSNP:746296901
700 700 a, c dbSNP:779127500
704 704 a, t dbSNP:757542604
705 705 a, g dbSNP:753955515
707 707 g, t dbSNP:764244520
710 710 c, t dbSNP:55853245
721 721 c, t dbSNP:752764608
733 733 a, c dbSNP:766361503
735 735 g, t dbSNP:762854508
738 738 a, c dbSNP:376936851
740 740 c, t dbSNP:199727473
750 750 c, t dbSNP:761551499
752 752 c, t dbSNP:776091871
755 755 c, t dbSNP:768286622
756 756 a, g dbSNP:746585235
779 779 a, g dbSNP:148169153
783 783 c, t dbSNP:143839858
784 784 a, g dbSNP:746331571
787 787 g, t dbSNP:121908491
791 791 c, t dbSNP:372968907
792 792 a, g, t dbSNP:749526870
797 797 a, g dbSNP:777920643
806 806 a, c, g dbSNP:148979783
809 809 a, g, t