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LGI1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol LGI1
Entrez Gene ID 9211
Full Name leucine-rich, glioma inactivated 1
Synonyms ADLTE, ADPAEF, ADPEAF, EPITEMPIN, EPT, ETL1, IB1099
General protein information
Preferred Names
leucine-rich glioma-inactivated protein 1
Names
leucine-rich glioma-inactivated protein 1
epitempin-1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q24

Summary This gene encodes a member of the secreted leucine-rich repeat (LRR) superfamily and shares homology with members of the SLIT protein family. The encoded protein may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. This gene is rearranged as a result of translocations in glioblastoma cell lines, and it is frequently down-regulated or rearranged in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]. lac of sum
Disorder MIM:

604619

Disorder Html: Epilepsy, partial, with auditory features, 600512 (3)





The following LGI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LGI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19654 NM_005097 Homo sapiens leucine-rich, glioma inactivated 1 (LGI1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.30
$379.00
OHu62623 NM_001308276 Homo sapiens leucine-rich, glioma inactivated 1 (LGI1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439.00
OHu62624 NM_001308275 Homo sapiens leucine-rich, glioma inactivated 1 (LGI1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu19654
Accession Version NM_005097.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1674bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 05-MAY-2015
Organism Homo sapiens (human)
Product leucine-rich glioma-inactivated protein 1 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC326684.1, AY358885.1, DB546258.1 and H05384.1. This sequence is a reference standard in the RefSeqGene project. On Apr 28, 2015 this sequence version replaced gi:171846229. Summary: This gene encodes a member of the secreted leucine-rich repeat (LRR) superfamily and shares homology with members of the SLIT protein family. The encoded protein may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. This gene is rearranged as a result of translocations in glioblastoma cell lines, and it is frequently down-regulated or rearranged in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022500.1, AF055636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGAATCAG AAAGAAGCAA AAGGATGGGA AATGCCTGCA TTCCCCTGAA AAGAATTGCT 
TATTTCCTAT GTCTCTTATC TGCGCTTTTG CTGACTGAGG GGAAGAAACC AGCGAAGCCA
AAATGCCCTG CCGTGTGTAC TTGTACCAAA GATAATGCTT TATGTGAGAA TGCCAGATCC
ATTCCACGCA CCGTTCCTCC TGATGTTATC TCATTATCCT TTGTGAGATC TGGTTTTACT
GAAATCTCAG AAGGGAGTTT TTTATTCACG CCATCGCTGC AGCTCTTGTT ATTCACATCG
AACTCCTTTG ATGTGATCAG TGATGATGCT TTTATTGGTC TTCCACATCT AGAGTATTTA
TTCATAGAAA ACAACAACAT CAAGTCAATT TCAAGACATA CTTTCCGGGG ACTAAAGTCA
TTAATTCACT TGAGCCTTGC AAACAACAAT CTCCAGACAC TCCCAAAAGA TATTTTCAAA
GGCCTGGATT CTTTAACAAA TGTGGACCTG AGGGGTAATT CATTTAATTG TGACTGTAAA
CTGAAATGGC TAGTGGAATG GCTTGGCCAC ACCAATGCAA CTGTTGAAGA CATCTACTGC
GAAGGCCCCC CAGAATACAA GAAGCGCAAA ATCAATAGTC TCTCCTCGAA GGATTTCGAT
TGCATCATTA CAGAATTTGC AAAGTCTCAA GACCTGCCTT ATCAATCATT GTCCATAGAC
ACTTTTTCTT ATTTGAATGA TGAGTATGTA GTCATCGCTC AGCCTTTTAC TGGAAAATGC
ATTTTCCTTG AATGGGACCA TGTGGAAAAG ACCTTCCGGA ATTATGACAA CATTACAGGC
ACATCCACTG TAGTATGCAA GCCTATAGTC ATTGAAACTC AGCTCTATGT TATTGTGGCC
CAGCTGTTTG GTGGCTCTCA CATCTATAAG CGAGACAGTT TTGCAAATAA ATTCATAAAA
ATCCAGGATA TTGAAATTCT CAAAATCCGA AAACCCAATG ACATTGAAAC ATTCAAGATT
GAAAACAACT GGTACTTTGT TGTTGCTGAC AGTTCAAAAG CTGGTTTTAC TACCATTTAC
AAATGGAACG GAAACGGATT CTACTCCCAT CAATCCTTAC ACGCGTGGTA CAGGGACACT
GATGTGGAAT ATCTAGAAAT AGTCAGAACA CCTCAGACAC TCAGAACGCC TCATTTAATT
CTGTCTAGTA GTTCCCAGCG TCCTGTAATT TATCAGTGGA ACAAAGCAAC ACAATTATTC
ACTAACCAAA CTGACATTCC TAACATGGAG GATGTGTACG CAGTGAAGCA CTTCTCAGTG
AAAGGGGACG TGTACATTTG CTTGACAAGA TTCATTGGTG ATTCCAAAGT CATGAAATGG
GGAGGCTCCT CGTTCCAGGA TATTCAGAGG ATGCCATCGC GAGGATCCAT GGTGTTCCAG
CCTCTTCAAA TAAATAATTA CCAATATGCA ATTCTTGGAA GTGATTACTC CTTTACTCAA
GTGTATAACT GGGATGCAGA GAAAGCCAAA TTTGTGAAAT TTCAGGAATT AAATGTTCAG
GCACCAAGAT CATTCACACA TGTGTCCATT AATAAGCGTA ATTTTCTTTT TGCTTCCAGT
TTTAAGGGAA ATACACAGAT TTACAAACAT GTCATAGTTG ACTTAAGCGC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_005088.1
CDS337..2010
Misc Feature(1)211..213(+)
Misc Feature(2)547..612(+)
Misc Feature(3)607..789(+)
Misc Feature(4)610..675(+)
Misc Feature(5)613..684(+)
Misc Feature(6)679..801(+)
Misc Feature(7)682..747(+)
Misc Feature(8)685..756(+)
Misc Feature(9)754..819(+)
Misc Feature(10)757..828(+)
Misc Feature(11)829..867(+)
Misc Feature(12)853..999(+)
Misc Feature(13)1006..1137(+)
Misc Feature(14)1006..1137(+)
Misc Feature(15)1144..1275(+)
Misc Feature(16)1144..1275(+)
Misc Feature(17)1282..1428(+)
Misc Feature(18)1282..1428(+)
Misc Feature(19)1429..1581(+)
Misc Feature(20)1429..1578(+)
Misc Feature(21)1588..1722(+)
Misc Feature(22)1588..1722(+)
Misc Feature(23)1723..1854(+)
Misc Feature(24)1723..1854(+)
Misc Feature(25)1861..1992(+)
Misc Feature(26)1864..1983(+)
Exon (1)1..551
Gene:LGI1
Gene Synonym:
Exon (2)552..623
Gene:LGI1
Gene Synonym:
Exon (3)624..695
Gene:LGI1
Gene Synonym:
Exon (4)696..767
Gene:LGI1
Gene Synonym:
Exon (5)768..839
Gene:LGI1
Gene Synonym:
Exon (6)840..1009
Gene:LGI1
Gene Synonym:
Exon (7)1010..1174
Gene:LGI1
Gene Synonym:
Exon (8)1175..2381
Gene:LGI1
Gene Synonym:
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_005097
Organism Homo sapiens (human)
Definition Homo sapiens leucine-rich, glioma inactivated 1 (LGI1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005097

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGAATCAG AAAGAAGCAA AAGGATGGGA AATGCCTGCA TTCCCCTGAA AAGAATTGCT 
TATTTCCTAT GTCTCTTATC TGCGCTTTTG CTGACTGAGG GGAAGAAACC AGCGAAGCCA
AAATGCCCTG CCGTGTGTAC TTGTACCAAA GATAATGCTT TATGTGAGAA TGCCAGATCC
ATTCCACGCA CCGTTCCTCC TGATGTTATC TCATTATCCT TTGTGAGATC TGGTTTTACT
GAAATCTCAG AAGGGAGTTT TTTATTCACG CCATCGCTGC AGCTCTTGTT ATTCACATCG
AACTCCTTTG ATGTGATCAG TGATGATGCT TTTATTGGTC TTCCACATCT AGAGTATTTA
TTCATAGAAA ACAACAACAT CAAGTCAATT TCAAGACATA CTTTCCGGGG ACTAAAGTCA
TTAATTCACT TGAGCCTTGC AAACAACAAT CTCCAGACAC TCCCAAAAGA TATTTTCAAA
GGCCTGGATT CTTTAACAAA TGTGGACCTG AGGGGTAATT CATTTAATTG TGACTGTAAA
CTGAAATGGC TAGTGGAATG GCTTGGCCAC ACCAATGCAA CTGTTGAAGA CATCTACTGC
GAAGGCCCCC CAGAATACAA GAAGCGCAAA ATCAATAGTC TCTCCTCGAA GGATTTCGAT
TGCATCATTA CAGAATTTGC AAAGTCTCAA GACCTGCCTT ATCAATCATT GTCCATAGAC
ACTTTTTCTT ATTTGAATGA TGAGTATGTA GTCATCGCTC AGCCTTTTAC TGGAAAATGC
ATTTTCCTTG AATGGGACCA TGTGGAAAAG ACCTTCCGGA ATTATGACAA CATTACAGGC
ACATCCACTG TAGTATGCAA GCCTATAGTC ATTGAAACTC AGCTCTATGT TATTGTGGCC
CAGCTGTTTG GTGGCTCTCA CATCTATAAG CGAGACAGTT TTGCAAATAA ATTCATAAAA
ATCCAGGATA TTGAAATTCT CAAAATCCGA AAACCCAATG ACATTGAAAC ATTCAAGATT
GAAAACAACT GGTACTTTGT TGTTGCTGAC AGTTCAAAAG CTGGTTTTAC TACCATTTAC
AAATGGAACG GAAACGGATT CTACTCCCAT CAATCCTTAC ACGCGTGGTA CAGGGACACT
GATGTGGAAT ATCTAGAAAT AGTCAGAACA CCTCAGACAC TCAGAACGCC TCATTTAATT
CTGTCTAGTA GTTCCCAGCG TCCTGTAATT TATCAGTGGA ACAAAGCAAC ACAATTATTC
ACTAACCAAA CTGACATTCC TAACATGGAG GATGTGTACG CAGTGAAGCA CTTCTCAGTG
AAAGGGGACG TGTACATTTG CTTGACAAGA TTCATTGGTG ATTCCAAAGT CATGAAATGG
GGAGGCTCCT CGTTCCAGGA TATTCAGAGG ATGCCATCGC GAGGATCCAT GGTGTTCCAG
CCTCTTCAAA TAAATAATTA CCAATATGCA ATTCTTGGAA GTGATTACTC CTTTACTCAA
GTGTATAACT GGGATGCAGA GAAAGCCAAA TTTGTGAAAT TTCAGGAATT AAATGTTCAG
GCACCAAGAT CATTCACACA TGTGTCCATT AATAAGCGTA ATTTTCTTTT TGCTTCCAGT
TTTAAGGGAA ATACACAGAT TTACAAACAT GTCATAGTTG ACTTAAGCGC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62623
Accession Version NM_001308276.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 05-MAY-2015
Organism Homo sapiens (human)
Product leucine-rich glioma-inactivated protein 1 isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC326684.1, AK303956.1, AF055636.1, DB546258.1 and H05384.1. Summary: This gene encodes a member of the secreted leucine-rich repeat (LRR) superfamily and shares homology with members of the SLIT protein family. The encoded protein may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. This gene is rearranged as a result of translocations in glioblastoma cell lines, and it is frequently down-regulated or rearranged in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]. Transcript Variant: This variant (3) lacks two in-frame exons in the 5' coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303956.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2147975 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGAATCAG AAAGAAGCAA AAGGATGGGA AATGCCTGCA TTCCCCTGAA AAGAATTGCT 
TATTTCCTAT GTCTCTTATC TGCGCTTTTG CTGACTGAGG GGAAGAAACC AGCGAAGCCA
AAATGCCCTG CCGTGTGTAC TTGTACCAAA GATAATGCTT TATGTGAGAA TGCCAGATCC
ATTCCACGCA CCGTTCCTCC TGATGTTATC TCATTATCCT TTGTGAGATC TGGTTTTACT
GAAATCTCAG AAGGGAGTTT TTTATTCACG CCATCGCTGC AGCTCTTGAG CCTTGCAAAC
AACAATCTCC AGACACTCCC AAAAGATATT TTCAAAGGCC TGGATTCTTT AACAAATGTG
GACCTGAGGG GTAATTCATT TAATTGTGAC TGTAAACTGA AATGGCTAGT GGAATGGCTT
GGCCACACCA ATGCAACTGT TGAAGACATC TACTGCGAAG GCCCCCCAGA ATACAAGAAG
CGCAAAATCA ATAGTCTCTC CTCGAAGGAT TTCGATTGCA TCATTACAGA ATTTGCAAAG
TCTCAAGACC TGCCTTATCA ATCATTGTCC ATAGACACTT TTTCTTATTT GAATGATGAG
TATGTAGTCA TCGCTCAGCC TTTTACTGGA AAATGCATTT TCCTTGAATG GGACCATGTG
GAAAAGACCT TCCGGAATTA TGACAACATT ACAGGCACAT CCACTGTAGT ATGCAAGCCT
ATAGTCATTG AAACTCAGCT CTATGTTATT GTGGCCCAGC TGTTTGGTGG CTCTCACATC
TATAAGCGAG ACAGTTTTGC AAATAAATTC ATAAAAATCC AGGATATTGA AATTCTCAAA
ATCCGAAAAC CCAATGACAT TGAAACATTC AAGATTGAAA ACAACTGGTA CTTTGTTGTT
GCTGACAGTT CAAAAGCTGG TTTTACTACC ATTTACAAAT GGAACGGAAA CGGATTCTAC
TCCCATCAAT CCTTACACGC GTGGTACAGG GACACTGATG TGGAATATCT AGAAATAGTC
AGAACACCTC AGACACTCAG AACGCCTCAT TTAATTCTGT CTAGTAGTTC CCAGCGTCCT
GTAATTTATC AGTGGAACAA AGCAACACAA TTATTCACTA ACCAAACTGA CATTCCTAAC
ATGGAGGATG TGTACGCAGT GAAGCACTTC TCAGTGAAAG GGGACGTGTA CATTTGCTTG
ACAAGATTCA TTGGTGATTC CAAAGTCATG AAATGGGGAG GCTCCTCGTT CCAGGATATT
CAGAGGATGC CATCGCGAGG ATCCATGGTG TTCCAGCCTC TTCAAATAAA TAATTACCAA
TATGCAATTC TTGGAAGTGA TTACTCCTTT ACTCAAGTGT ATAACTGGGA TGCAGAGAAA
GCCAAATTTG TGAAATTTCA GGAATTAAAT GTTCAGGCAC CAAGATCATT CACACATGTG
TCCATTAATA AGCGTAATTT TCTTTTTGCT TCCAGTTTTA AGGGAAATAC ACAGATTTAC
AAACATGTCA TAGTTGACTT AAGCGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001295205.1
CDS337..1866
Misc Feature(1)211..213(+)
Misc Feature(2)547..717(+)
Misc Feature(3)607..675(+)
Misc Feature(4)613..684(+)
Misc Feature(5)685..723(+)
Misc Feature(6)709..855(+)
Misc Feature(7)862..993(+)
Misc Feature(8)1000..1131(+)
Misc Feature(9)1138..1284(+)
Misc Feature(10)1285..1434(+)
Misc Feature(11)1444..1578(+)
Misc Feature(12)1579..1710(+)
Misc Feature(13)1720..1839(+)
Exon (1)1..551
Gene:LGI1
Gene Synonym:
Exon (2)552..623
Gene:LGI1
Gene Synonym:
Exon (3)624..695
Gene:LGI1
Gene Synonym:
Exon (4)696..865
Gene:LGI1
Gene Synonym:
Exon (5)866..1030
Gene:LGI1
Gene Synonym:
Exon (6)1031..2237
Gene:LGI1
Gene Synonym:
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_001308276
Organism Homo sapiens (human)
Definition Homo sapiens leucine-rich, glioma inactivated 1 (LGI1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001308276

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGAATCAG AAAGAAGCAA AAGGATGGGA AATGCCTGCA TTCCCCTGAA AAGAATTGCT 
TATTTCCTAT GTCTCTTATC TGCGCTTTTG CTGACTGAGG GGAAGAAACC AGCGAAGCCA
AAATGCCCTG CCGTGTGTAC TTGTACCAAA GATAATGCTT TATGTGAGAA TGCCAGATCC
ATTCCACGCA CCGTTCCTCC TGATGTTATC TCATTATCCT TTGTGAGATC TGGTTTTACT
GAAATCTCAG AAGGGAGTTT TTTATTCACG CCATCGCTGC AGCTCTTGAG CCTTGCAAAC
AACAATCTCC AGACACTCCC AAAAGATATT TTCAAAGGCC TGGATTCTTT AACAAATGTG
GACCTGAGGG GTAATTCATT TAATTGTGAC TGTAAACTGA AATGGCTAGT GGAATGGCTT
GGCCACACCA ATGCAACTGT TGAAGACATC TACTGCGAAG GCCCCCCAGA ATACAAGAAG
CGCAAAATCA ATAGTCTCTC CTCGAAGGAT TTCGATTGCA TCATTACAGA ATTTGCAAAG
TCTCAAGACC TGCCTTATCA ATCATTGTCC ATAGACACTT TTTCTTATTT GAATGATGAG
TATGTAGTCA TCGCTCAGCC TTTTACTGGA AAATGCATTT TCCTTGAATG GGACCATGTG
GAAAAGACCT TCCGGAATTA TGACAACATT ACAGGCACAT CCACTGTAGT ATGCAAGCCT
ATAGTCATTG AAACTCAGCT CTATGTTATT GTGGCCCAGC TGTTTGGTGG CTCTCACATC
TATAAGCGAG ACAGTTTTGC AAATAAATTC ATAAAAATCC AGGATATTGA AATTCTCAAA
ATCCGAAAAC CCAATGACAT TGAAACATTC AAGATTGAAA ACAACTGGTA CTTTGTTGTT
GCTGACAGTT CAAAAGCTGG TTTTACTACC ATTTACAAAT GGAACGGAAA CGGATTCTAC
TCCCATCAAT CCTTACACGC GTGGTACAGG GACACTGATG TGGAATATCT AGAAATAGTC
AGAACACCTC AGACACTCAG AACGCCTCAT TTAATTCTGT CTAGTAGTTC CCAGCGTCCT
GTAATTTATC AGTGGAACAA AGCAACACAA TTATTCACTA ACCAAACTGA CATTCCTAAC
ATGGAGGATG TGTACGCAGT GAAGCACTTC TCAGTGAAAG GGGACGTGTA CATTTGCTTG
ACAAGATTCA TTGGTGATTC CAAAGTCATG AAATGGGGAG GCTCCTCGTT CCAGGATATT
CAGAGGATGC CATCGCGAGG ATCCATGGTG TTCCAGCCTC TTCAAATAAA TAATTACCAA
TATGCAATTC TTGGAAGTGA TTACTCCTTT ACTCAAGTGT ATAACTGGGA TGCAGAGAAA
GCCAAATTTG TGAAATTTCA GGAATTAAAT GTTCAGGCAC CAAGATCATT CACACATGTG
TCCATTAATA AGCGTAATTT TCTTTTTGCT TCCAGTTTTA AGGGAAATAC ACAGATTTAC
AAACATGTCA TAGTTGACTT AAGCGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62624
Accession Version NM_001308275.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 876bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product leucine-rich glioma-inactivated protein 1 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC326684.1, AF473548.1, DB546258.1 and H05384.1. On Apr 28, 2015 this sequence version replaced gi:530394597. Summary: This gene encodes a member of the secreted leucine-rich repeat (LRR) superfamily and shares homology with members of the SLIT protein family. The encoded protein may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. This gene is rearranged as a result of translocations in glioblastoma cell lines, and it is frequently down-regulated or rearranged in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which leads to a frameshift compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF473548.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAATCAG AAAGAAGCAA AAGGATGGGA AATGCCTGCA TTCCCCTGAA AAGAATTGCT 
TATTTCCTAT GTCTCTTATC TGCGCTTTTG CTGACTGAGG GGAAGAAACC AGCGAAGCCA
AAATGCCCTG CCGTGTGTAC TTGTACCAAA GATAATGCTT TATGTGAGAA TGCCAGATCC
ATTCCACGCA CCGTTCCTCC TGATGTTATC TCATTATCCT TTGTGAGATC TGGTTTTACT
GAAATCTCAG AAGGGAGTTT TTTATTCACG CCATCGCTGC AGCTCTTGTT ATTCACATCG
AACTCCTTTG ATGTGATCAG TGATGATGCT TTTATTGGTC TTCCACATCT AGAGTATTTA
TTCATAGAAA ACAACAACAT CAAGTCAATT TCAAGACATA CTTTCCGGGG ACTAAAGTCA
TTAATTCACT TGAGCCTTGC AAACAACAAT CTCCAGACAC TCCCAAAAGA TATTTTCAAA
GGCCTGGATT CTTTAACAAA TGTGGACCTG AGGGGTAATT CATTTAATTG TGACTGTAAA
CTGAAATGGC TAGTGGAATG GCTTGGCCAC ACCAATGCAA CTGTTGAAGA CATCTACTGC
GAAGGCCCCC CAGAATACAA GAAGCGCAAA ATCAATAGTC TCTCCTCGAA GGATTTCGAT
TGCATCATTA CAGAATTTGC AAAGTCTCAA GACCTGCCTT ATCAATCATT GTCCATAGAC
ACTTTTTCTT ATTTGAATGA TGAGTATGTA GTCATCGCTC AGCCTTTTAC TGGAAAATGC
ATTTTCCTTG AATGGGACCA TGTGGAAAAG ACCTTCCGGA ATTATGACAA CATTACAGTT
TTAAGGGAAA TACACAGATT TACAAACATG TCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001295204.1
CDS337..1212
Misc Feature(1)211..213(+)
Misc Feature(2)547..612(+)
Misc Feature(3)607..789(+)
Misc Feature(4)610..675(+)
Misc Feature(5)613..684(+)
Misc Feature(6)679..801(+)
Misc Feature(7)682..747(+)
Misc Feature(8)685..756(+)
Misc Feature(9)754..819(+)
Misc Feature(10)757..828(+)
Misc Feature(11)829..867(+)
Misc Feature(12)853..999(+)
Misc Feature(13)1006..1137(+)
Misc Feature(14)1006..1137(+)
Exon (1)1..551
Gene:LGI1
Gene Synonym:
Exon (2)552..623
Gene:LGI1
Gene Synonym:
Exon (3)624..695
Gene:LGI1
Gene Synonym:
Exon (4)696..767
Gene:LGI1
Gene Synonym:
Exon (5)768..839
Gene:LGI1
Gene Synonym:
Exon (6)840..1009
Gene:LGI1
Gene Synonym:
Exon (7)1010..1174
Gene:LGI1
Gene Synonym:
Exon (8)1175..1600
Gene:LGI1
Gene Synonym:
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_001308275
Organism Homo sapiens (human)
Definition Homo sapiens leucine-rich, glioma inactivated 1 (LGI1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001308275

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAATCAG AAAGAAGCAA AAGGATGGGA AATGCCTGCA TTCCCCTGAA AAGAATTGCT 
TATTTCCTAT GTCTCTTATC TGCGCTTTTG CTGACTGAGG GGAAGAAACC AGCGAAGCCA
AAATGCCCTG CCGTGTGTAC TTGTACCAAA GATAATGCTT TATGTGAGAA TGCCAGATCC
ATTCCACGCA CCGTTCCTCC TGATGTTATC TCATTATCCT TTGTGAGATC TGGTTTTACT
GAAATCTCAG AAGGGAGTTT TTTATTCACG CCATCGCTGC AGCTCTTGTT ATTCACATCG
AACTCCTTTG ATGTGATCAG TGATGATGCT TTTATTGGTC TTCCACATCT AGAGTATTTA
TTCATAGAAA ACAACAACAT CAAGTCAATT TCAAGACATA CTTTCCGGGG ACTAAAGTCA
TTAATTCACT TGAGCCTTGC AAACAACAAT CTCCAGACAC TCCCAAAAGA TATTTTCAAA
GGCCTGGATT CTTTAACAAA TGTGGACCTG AGGGGTAATT CATTTAATTG TGACTGTAAA
CTGAAATGGC TAGTGGAATG GCTTGGCCAC ACCAATGCAA CTGTTGAAGA CATCTACTGC
GAAGGCCCCC CAGAATACAA GAAGCGCAAA ATCAATAGTC TCTCCTCGAA GGATTTCGAT
TGCATCATTA CAGAATTTGC AAAGTCTCAA GACCTGCCTT ATCAATCATT GTCCATAGAC
ACTTTTTCTT ATTTGAATGA TGAGTATGTA GTCATCGCTC AGCCTTTTAC TGGAAAATGC
ATTTTCCTTG AATGGGACCA TGTGGAAAAG ACCTTCCGGA ATTATGACAA CATTACAGTT
TTAAGGGAAA TACACAGATT TACAAACATG TCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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