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LARGE cDNA ORF clone, Homo sapiens (human)

Gene Symbol LARGE
Entrez Gene ID 9215
Full Name like-glycosyltransferase
Synonyms MDC1D, MDDGA6, MDDGB6
General protein information
Preferred Names
glycosyltransferase-like protein LARGE1
Names
glycosyltransferase-like protein LARGE1
like-acetylglucosaminyltransferase
acetylglucosaminyltransferase-like 1A
acetylglucosaminyltransferase-like protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q12.3

Summary This gene, which is one of the largest in the human genome, encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. It may also be involved in the addition of a repeated disaccharide unit. Mutations in this gene cause MDC1D, a novel form of congenital muscular dystrophy with severe mental retardation and abnormal glycosylation of alpha-dystroglycan. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

603590

Disorder Html: Muscular dystrophy-dystroglycanopathy (congenital with brain and eye

mRNA and Protein(s)

mRNA Protein Name
XM_005261831 XP_005261888 glycosyltransferase-like protein LARGE1 isoform X1
XM_011530510 XP_011528812 glycosyltransferase-like protein LARGE1 isoform X1
XM_005261832 XP_005261889 glycosyltransferase-like protein LARGE1 isoform X1
XM_011530511 XP_011528813 glycosyltransferase-like protein LARGE1 isoform X2
XM_011530512 XP_011528814 glycosyltransferase-like protein LARGE1 isoform X3
XM_011530513 XP_011528815 glycosyltransferase-like protein LARGE1 isoform X4
XM_011530514 XP_011528816 glycosyltransferase-like protein LARGE1 isoform X5
NM_004737 NP_004728 glycosyltransferase-like protein LARGE1
NM_133642 NP_598397 glycosyltransferase-like protein LARGE1


Homo sapiens (human) LARGE NP_004728.1
Pan troglodytes (chimpanzee) LARGE XP_001154608.1
Macaca mulatta (Rhesus monkey) LARGE XP_001113031.1
Canis lupus familiaris (dog) LARGE XP_005625982.1
Bos taurus (cattle) LARGE NP_001192517.1
Mus musculus (house mouse) Large NP_034817.1
Gallus gallus (chicken) LARGE NP_001004383.1
Danio rerio (zebrafish) large NP_001004537.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100498555 XP_002943094.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LARGE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LARGE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu28267
XM_005261831 PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu28267
XM_011530510 PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu28267
XM_005261832 PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu62626 XM_011530511 PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu62627 XM_011530512 PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu62628 XM_011530513 PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu62629 XM_011530514 PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu28267
NM_004737 Homo sapiens like-glycosyltransferase (LARGE), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu28267
NM_133642 Homo sapiens like-glycosyltransferase (LARGE), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu28267
Clone ID Related Accession (Same CDS sequence) NM_004737 , NM_133642 , XM_005261831 , XM_005261832 , XM_011530510
Accession Version XM_005261831.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glycosyltransferase-like protein LARGE1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530420562. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GGTCATTCAC
TGGAACTCCC CCAAGAAGCT CCGGGTGAAG AACAAGCATG TGGAGTTTTT TCGCAACCTC
TACCTGACCT TCCTGGAGTA TGACGGCAAT CTTCTGAGGC GGGAACTGTT TGGCTGCCCC
AGTGAGGCTG ATGTCAACAG TGAAAACCTC CAGAAGCAGC TGTCTGAGCT GGACGAGGAC
GACCTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACCGCACCCA CCTGTACTTC
CTGCACTACG AGTATGAGCC TGCAGCAGAC AGCACGGACG TCACCCTGGT CGCTCAGCTG
TCCATGGACA GGCTCCAGAT GCTGGAGGCC ATCTGCAAGC ACTGGGAGGG GCCCATCAGC
CTGGCCCTCT ACCTGTCAGA CGCCGAGGCC CAGCAGTTCC TCCGCTACGC ACAGGGCTCT
GAGGTGCTTA TGAGCCGCCA CAACGTGGGC TACCACATCG TGTACAAGGA GGGCCAGTTC
TACCCCGTGA ACCTGCTGCG CAACGTGGCC ATGAAGCACA TCAGCACTCC CTACATGTTC
CTGTCTGACA TTGACTTCCT GCCCATGTAT GGGCTCTATG AGTACCTCAG GAAGTCTGTC
ATCCAGCTCG ATCTTGCCAA CACCAAGAAA GCAATGATTG TCCCCGCGTT CGAGACACTG
CGCTACCGGC TGTCCTTCCC CAAGTCAAAA GCGGAGTTGC TGTCAATGCT GGACATGGGG
ACCCTCTTCA CATTCAGGTA CCACGTCTGG ACGAAAGGCC ACGCACCCAC AAACTTCGCC
AAGTGGCGGA CCGCCACCAC GCCTTACCGG GTTGAGTGGG AGGCCGATTT TGAGCCGTAT
GTTGTTGTGA GACGTGACTG CCCGGAGTAC GACCGGAGGT TTGTAGGCTT TGGCTGGAAC
AAAGTGGCTC ATATCATGGA GCTGGATGTG CAGGAGTATG AGTTCATTGT GCTGCCCAAC
GCCTACATGA TCCACATGCC TCATGCCCCC AGCTTCGACA TTACCAAGTT CCGTTCCAAC
AAGCAATACC GCATCTGTCT CAAAACCCTC AAGGAAGAGT TTCAGCAGGA CATGTCCCGC
CGCTACGGCT TTGCTGCCCT GAAATATCTC ACAGCCGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005261888.1
CDS426..2696
Misc Feature(1)831..>1583(+)
Misc Feature(2)837..1676(+)
Misc Feature(3)852..1583(+)
Misc Feature(4)1149..1565(+)
Misc Feature(5)1842..2654(+)
Translation

Target ORF information:

RefSeq Version XM_005261831
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005261831

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GGTCATTCAC
TGGAACTCCC CCAAGAAGCT CCGGGTGAAG AACAAGCATG TGGAGTTTTT TCGCAACCTC
TACCTGACCT TCCTGGAGTA TGACGGCAAT CTTCTGAGGC GGGAACTGTT TGGCTGCCCC
AGTGAGGCTG ATGTCAACAG TGAAAACCTC CAGAAGCAGC TGTCTGAGCT GGACGAGGAC
GACCTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACCGCACCCA CCTGTACTTC
CTGCACTACG AGTATGAGCC TGCAGCAGAC AGCACGGACG TCACCCTGGT CGCTCAGCTG
TCCATGGACA GGCTCCAGAT GCTGGAGGCC ATCTGCAAGC ACTGGGAGGG GCCCATCAGC
CTGGCCCTCT ACCTGTCAGA CGCCGAGGCC CAGCAGTTCC TCCGCTACGC ACAGGGCTCT
GAGGTGCTTA TGAGCCGCCA CAACGTGGGC TACCACATCG TGTACAAGGA GGGCCAGTTC
TACCCCGTGA ACCTGCTGCG CAACGTGGCC ATGAAGCACA TCAGCACTCC CTACATGTTC
CTGTCTGACA TTGACTTCCT GCCCATGTAT GGGCTCTATG AGTACCTCAG GAAGTCTGTC
ATCCAGCTCG ATCTTGCCAA CACCAAGAAA GCAATGATTG TCCCCGCGTT CGAGACACTG
CGCTACCGGC TGTCCTTCCC CAAGTCAAAA GCGGAGTTGC TGTCAATGCT GGACATGGGG
ACCCTCTTCA CATTCAGGTA CCACGTCTGG ACGAAAGGCC ACGCACCCAC AAACTTCGCC
AAGTGGCGGA CCGCCACCAC GCCTTACCGG GTTGAGTGGG AGGCCGATTT TGAGCCGTAT
GTTGTTGTGA GACGTGACTG CCCGGAGTAC GACCGGAGGT TTGTAGGCTT TGGCTGGAAC
AAAGTGGCTC ATATCATGGA GCTGGATGTG CAGGAGTATG AGTTCATTGT GCTGCCCAAC
GCCTACATGA TCCACATGCC TCATGCCCCC AGCTTCGACA TTACCAAGTT CCGTTCCAAC
AAGCAATACC GCATCTGTCT CAAAACCCTC AAGGAAGAGT TTCAGCAGGA CATGTCCCGC
CGCTACGGCT TTGCTGCCCT GAAATATCTC ACAGCCGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28267
Clone ID Related Accession (Same CDS sequence) NM_004737 , NM_133642 , XM_005261831 , XM_005261832 , XM_011530510
Accession Version XM_011530510.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glycosyltransferase-like protein LARGE1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GGTCATTCAC
TGGAACTCCC CCAAGAAGCT CCGGGTGAAG AACAAGCATG TGGAGTTTTT TCGCAACCTC
TACCTGACCT TCCTGGAGTA TGACGGCAAT CTTCTGAGGC GGGAACTGTT TGGCTGCCCC
AGTGAGGCTG ATGTCAACAG TGAAAACCTC CAGAAGCAGC TGTCTGAGCT GGACGAGGAC
GACCTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACCGCACCCA CCTGTACTTC
CTGCACTACG AGTATGAGCC TGCAGCAGAC AGCACGGACG TCACCCTGGT CGCTCAGCTG
TCCATGGACA GGCTCCAGAT GCTGGAGGCC ATCTGCAAGC ACTGGGAGGG GCCCATCAGC
CTGGCCCTCT ACCTGTCAGA CGCCGAGGCC CAGCAGTTCC TCCGCTACGC ACAGGGCTCT
GAGGTGCTTA TGAGCCGCCA CAACGTGGGC TACCACATCG TGTACAAGGA GGGCCAGTTC
TACCCCGTGA ACCTGCTGCG CAACGTGGCC ATGAAGCACA TCAGCACTCC CTACATGTTC
CTGTCTGACA TTGACTTCCT GCCCATGTAT GGGCTCTATG AGTACCTCAG GAAGTCTGTC
ATCCAGCTCG ATCTTGCCAA CACCAAGAAA GCAATGATTG TCCCCGCGTT CGAGACACTG
CGCTACCGGC TGTCCTTCCC CAAGTCAAAA GCGGAGTTGC TGTCAATGCT GGACATGGGG
ACCCTCTTCA CATTCAGGTA CCACGTCTGG ACGAAAGGCC ACGCACCCAC AAACTTCGCC
AAGTGGCGGA CCGCCACCAC GCCTTACCGG GTTGAGTGGG AGGCCGATTT TGAGCCGTAT
GTTGTTGTGA GACGTGACTG CCCGGAGTAC GACCGGAGGT TTGTAGGCTT TGGCTGGAAC
AAAGTGGCTC ATATCATGGA GCTGGATGTG CAGGAGTATG AGTTCATTGT GCTGCCCAAC
GCCTACATGA TCCACATGCC TCATGCCCCC AGCTTCGACA TTACCAAGTT CCGTTCCAAC
AAGCAATACC GCATCTGTCT CAAAACCCTC AAGGAAGAGT TTCAGCAGGA CATGTCCCGC
CGCTACGGCT TTGCTGCCCT GAAATATCTC ACAGCCGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528812.1
CDS367..2637
Misc Feature(1)772..>1524(+)
Misc Feature(2)778..1617(+)
Misc Feature(3)793..1524(+)
Misc Feature(4)1090..1506(+)
Misc Feature(5)1783..2595(+)
Translation

Target ORF information:

RefSeq Version XM_011530510
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530510

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GGTCATTCAC
TGGAACTCCC CCAAGAAGCT CCGGGTGAAG AACAAGCATG TGGAGTTTTT TCGCAACCTC
TACCTGACCT TCCTGGAGTA TGACGGCAAT CTTCTGAGGC GGGAACTGTT TGGCTGCCCC
AGTGAGGCTG ATGTCAACAG TGAAAACCTC CAGAAGCAGC TGTCTGAGCT GGACGAGGAC
GACCTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACCGCACCCA CCTGTACTTC
CTGCACTACG AGTATGAGCC TGCAGCAGAC AGCACGGACG TCACCCTGGT CGCTCAGCTG
TCCATGGACA GGCTCCAGAT GCTGGAGGCC ATCTGCAAGC ACTGGGAGGG GCCCATCAGC
CTGGCCCTCT ACCTGTCAGA CGCCGAGGCC CAGCAGTTCC TCCGCTACGC ACAGGGCTCT
GAGGTGCTTA TGAGCCGCCA CAACGTGGGC TACCACATCG TGTACAAGGA GGGCCAGTTC
TACCCCGTGA ACCTGCTGCG CAACGTGGCC ATGAAGCACA TCAGCACTCC CTACATGTTC
CTGTCTGACA TTGACTTCCT GCCCATGTAT GGGCTCTATG AGTACCTCAG GAAGTCTGTC
ATCCAGCTCG ATCTTGCCAA CACCAAGAAA GCAATGATTG TCCCCGCGTT CGAGACACTG
CGCTACCGGC TGTCCTTCCC CAAGTCAAAA GCGGAGTTGC TGTCAATGCT GGACATGGGG
ACCCTCTTCA CATTCAGGTA CCACGTCTGG ACGAAAGGCC ACGCACCCAC AAACTTCGCC
AAGTGGCGGA CCGCCACCAC GCCTTACCGG GTTGAGTGGG AGGCCGATTT TGAGCCGTAT
GTTGTTGTGA GACGTGACTG CCCGGAGTAC GACCGGAGGT TTGTAGGCTT TGGCTGGAAC
AAAGTGGCTC ATATCATGGA GCTGGATGTG CAGGAGTATG AGTTCATTGT GCTGCCCAAC
GCCTACATGA TCCACATGCC TCATGCCCCC AGCTTCGACA TTACCAAGTT CCGTTCCAAC
AAGCAATACC GCATCTGTCT CAAAACCCTC AAGGAAGAGT TTCAGCAGGA CATGTCCCGC
CGCTACGGCT TTGCTGCCCT GAAATATCTC ACAGCCGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28267
Clone ID Related Accession (Same CDS sequence) NM_004737 , NM_133642 , XM_005261831 , XM_005261832 , XM_011530510
Accession Version XM_005261832.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glycosyltransferase-like protein LARGE1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530420564. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GGTCATTCAC
TGGAACTCCC CCAAGAAGCT CCGGGTGAAG AACAAGCATG TGGAGTTTTT TCGCAACCTC
TACCTGACCT TCCTGGAGTA TGACGGCAAT CTTCTGAGGC GGGAACTGTT TGGCTGCCCC
AGTGAGGCTG ATGTCAACAG TGAAAACCTC CAGAAGCAGC TGTCTGAGCT GGACGAGGAC
GACCTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACCGCACCCA CCTGTACTTC
CTGCACTACG AGTATGAGCC TGCAGCAGAC AGCACGGACG TCACCCTGGT CGCTCAGCTG
TCCATGGACA GGCTCCAGAT GCTGGAGGCC ATCTGCAAGC ACTGGGAGGG GCCCATCAGC
CTGGCCCTCT ACCTGTCAGA CGCCGAGGCC CAGCAGTTCC TCCGCTACGC ACAGGGCTCT
GAGGTGCTTA TGAGCCGCCA CAACGTGGGC TACCACATCG TGTACAAGGA GGGCCAGTTC
TACCCCGTGA ACCTGCTGCG CAACGTGGCC ATGAAGCACA TCAGCACTCC CTACATGTTC
CTGTCTGACA TTGACTTCCT GCCCATGTAT GGGCTCTATG AGTACCTCAG GAAGTCTGTC
ATCCAGCTCG ATCTTGCCAA CACCAAGAAA GCAATGATTG TCCCCGCGTT CGAGACACTG
CGCTACCGGC TGTCCTTCCC CAAGTCAAAA GCGGAGTTGC TGTCAATGCT GGACATGGGG
ACCCTCTTCA CATTCAGGTA CCACGTCTGG ACGAAAGGCC ACGCACCCAC AAACTTCGCC
AAGTGGCGGA CCGCCACCAC GCCTTACCGG GTTGAGTGGG AGGCCGATTT TGAGCCGTAT
GTTGTTGTGA GACGTGACTG CCCGGAGTAC GACCGGAGGT TTGTAGGCTT TGGCTGGAAC
AAAGTGGCTC ATATCATGGA GCTGGATGTG CAGGAGTATG AGTTCATTGT GCTGCCCAAC
GCCTACATGA TCCACATGCC TCATGCCCCC AGCTTCGACA TTACCAAGTT CCGTTCCAAC
AAGCAATACC GCATCTGTCT CAAAACCCTC AAGGAAGAGT TTCAGCAGGA CATGTCCCGC
CGCTACGGCT TTGCTGCCCT GAAATATCTC ACAGCCGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005261889.1
CDS381..2651
Misc Feature(1)786..>1538(+)
Misc Feature(2)792..1631(+)
Misc Feature(3)807..1538(+)
Misc Feature(4)1104..1520(+)
Misc Feature(5)1797..2609(+)
Translation

Target ORF information:

RefSeq Version XM_005261832
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005261832

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GGTCATTCAC
TGGAACTCCC CCAAGAAGCT CCGGGTGAAG AACAAGCATG TGGAGTTTTT TCGCAACCTC
TACCTGACCT TCCTGGAGTA TGACGGCAAT CTTCTGAGGC GGGAACTGTT TGGCTGCCCC
AGTGAGGCTG ATGTCAACAG TGAAAACCTC CAGAAGCAGC TGTCTGAGCT GGACGAGGAC
GACCTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACCGCACCCA CCTGTACTTC
CTGCACTACG AGTATGAGCC TGCAGCAGAC AGCACGGACG TCACCCTGGT CGCTCAGCTG
TCCATGGACA GGCTCCAGAT GCTGGAGGCC ATCTGCAAGC ACTGGGAGGG GCCCATCAGC
CTGGCCCTCT ACCTGTCAGA CGCCGAGGCC CAGCAGTTCC TCCGCTACGC ACAGGGCTCT
GAGGTGCTTA TGAGCCGCCA CAACGTGGGC TACCACATCG TGTACAAGGA GGGCCAGTTC
TACCCCGTGA ACCTGCTGCG CAACGTGGCC ATGAAGCACA TCAGCACTCC CTACATGTTC
CTGTCTGACA TTGACTTCCT GCCCATGTAT GGGCTCTATG AGTACCTCAG GAAGTCTGTC
ATCCAGCTCG ATCTTGCCAA CACCAAGAAA GCAATGATTG TCCCCGCGTT CGAGACACTG
CGCTACCGGC TGTCCTTCCC CAAGTCAAAA GCGGAGTTGC TGTCAATGCT GGACATGGGG
ACCCTCTTCA CATTCAGGTA CCACGTCTGG ACGAAAGGCC ACGCACCCAC AAACTTCGCC
AAGTGGCGGA CCGCCACCAC GCCTTACCGG GTTGAGTGGG AGGCCGATTT TGAGCCGTAT
GTTGTTGTGA GACGTGACTG CCCGGAGTAC GACCGGAGGT TTGTAGGCTT TGGCTGGAAC
AAAGTGGCTC ATATCATGGA GCTGGATGTG CAGGAGTATG AGTTCATTGT GCTGCCCAAC
GCCTACATGA TCCACATGCC TCATGCCCCC AGCTTCGACA TTACCAAGTT CCGTTCCAAC
AAGCAATACC GCATCTGTCT CAAAACCCTC AAGGAAGAGT TTCAGCAGGA CATGTCCCGC
CGCTACGGCT TTGCTGCCCT GAAATATCTC ACAGCCGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62626
Clone ID Related Accession (Same CDS sequence) XM_011530511
Accession Version XM_011530511.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glycosyltransferase-like protein LARGE1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GGTCATTCAC
TGGAACTCCC CCAAGAAGCT CCGGGTGAAG AACAAGCATG TGGAGTTTTT TCGCAACCTC
TACCTGACCT TCCTGGAGTA TGACGGCAAT CTTCTGAGGC GGGAACTGTT TGGCTGCCCC
AGTGAGGCTG ATGTCAACAG TGAAAACCTC CAGAAGCAGC TGTCTGAGCT GGACGAGGAC
GACCTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACCGCACCCA CCTGTACTTC
CTGCACTACG AGTATGAGCC TGCAGCAGAC AGCACGGACG TCACCCTGGT CGCTCAGCTG
TCCATGGACA GGAAGTCTGT CATCCAGCTC GATCTTGCCA ACACCAAGAA AGCAATGATT
GTCCCCGCGT TCGAGACACT GCGCTACCGG CTGTCCTTCC CCAAGTCAAA AGCGGAGTTG
CTGTCAATGC TGGACATGGG GACCCTCTTC ACATTCAGGT ACCACGTCTG GACGAAAGGC
CACGCACCCA CAAACTTCGC CAAGTGGCGG ACCGCCACCA CGCCTTACCG GGTTGAGTGG
GAGGCCGATT TTGAGCCGTA TGTTGTTGTG AGACGTGACT GCCCGGAGTA CGACCGGAGG
TTTGTAGGCT TTGGCTGGAA CAAAGTGGCT CATATCATGG AGCTGGATGT GCAGGAGTAT
GAGTTCATTG TGCTGCCCAA CGCCTACATG ATCCACATGC CTCATGCCCC CAGCTTCGAC
ATTACCAAGT TCCGTTCCAA CAAGCAATAC CGCATCTGTC TCAAAACCCT CAAGGAAGAG
TTTCAGCAGG ACATGTCCCG CCGCTACGGC TTTGCTGCCC TGAAATATCT CACAGCCGAG
AACAACAGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528813.1
CDS628..2619
Misc Feature(1)1033..>1785(+)
Misc Feature(2)1039..1878(+)
Misc Feature(3)1054..1785(+)
Misc Feature(4)1351..1767(+)
Misc Feature(5)2107..2577(+)
Translation

Target ORF information:

RefSeq Version XM_011530511
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530511

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GGTCATTCAC
TGGAACTCCC CCAAGAAGCT CCGGGTGAAG AACAAGCATG TGGAGTTTTT TCGCAACCTC
TACCTGACCT TCCTGGAGTA TGACGGCAAT CTTCTGAGGC GGGAACTGTT TGGCTGCCCC
AGTGAGGCTG ATGTCAACAG TGAAAACCTC CAGAAGCAGC TGTCTGAGCT GGACGAGGAC
GACCTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACCGCACCCA CCTGTACTTC
CTGCACTACG AGTATGAGCC TGCAGCAGAC AGCACGGACG TCACCCTGGT CGCTCAGCTG
TCCATGGACA GGAAGTCTGT CATCCAGCTC GATCTTGCCA ACACCAAGAA AGCAATGATT
GTCCCCGCGT TCGAGACACT GCGCTACCGG CTGTCCTTCC CCAAGTCAAA AGCGGAGTTG
CTGTCAATGC TGGACATGGG GACCCTCTTC ACATTCAGGT ACCACGTCTG GACGAAAGGC
CACGCACCCA CAAACTTCGC CAAGTGGCGG ACCGCCACCA CGCCTTACCG GGTTGAGTGG
GAGGCCGATT TTGAGCCGTA TGTTGTTGTG AGACGTGACT GCCCGGAGTA CGACCGGAGG
TTTGTAGGCT TTGGCTGGAA CAAAGTGGCT CATATCATGG AGCTGGATGT GCAGGAGTAT
GAGTTCATTG TGCTGCCCAA CGCCTACATG ATCCACATGC CTCATGCCCC CAGCTTCGAC
ATTACCAAGT TCCGTTCCAA CAAGCAATAC CGCATCTGTC TCAAAACCCT CAAGGAAGAG
TTTCAGCAGG ACATGTCCCG CCGCTACGGC TTTGCTGCCC TGAAATATCT CACAGCCGAG
AACAACAGCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62627
Clone ID Related Accession (Same CDS sequence) XM_011530512
Accession Version XM_011530512.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glycosyltransferase-like protein LARGE1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTTAATAT GGTCTGAAGT TTCCTGGATC CCCAATAAAC ATTACTCTGG GATTTATGGT 
CTGATGAAGC TTGTCCTGAC CAAGACTCTT CCTGCCAACC TGGAGAGAGT CATCGTCCTT
GACACGGATA TCACCTTTGC CACTGACATT GCAGAGCTGT GGGCTGTGTT CCACAAGTTC
AAAGGTCAGC AAGTCCTGGG CTTGGTGGAG AACCAGAGTG ACTGGTACCT TGGAAACCTG
TGGAAAAATC ACCGCCCATG GCCAGCCCTT GGAAGAGGCT ACAACACAGG GGTGATCCTG
TTACTTCTGG ATAAGCTGCG GAAGATGAAA TGGGAGCAGA TGTGGAGGCT GACCGCAGAG
AGGGAGCTCA TGGGCATGCT CTCTACATCC TTAGCTGACC AGGATATTTT CAATGCCGTC
ATCAAACAAA ACCCCTTCCT TGTGTACCAG CTCCCCTGCT TCTGGAATGT GCAGCTGTCA
GACCACACCC GCTCCGAGCA GTGCTACAGA GACGTGTCTG ATCTAAAGGT CATTCACTGG
AACTCCCCCA AGAAGCTCCG GGTGAAGAAC AAGCATGTGG AGTTTTTTCG CAACCTCTAC
CTGACCTTCC TGGAGTATGA CGGCAATCTT CTGAGGCGGG AACTGTTTGG CTGCCCCAGT
GAGGCTGATG TCAACAGTGA AAACCTCCAG AAGCAGCTGT CTGAGCTGGA CGAGGACGAC
CTGTGCTATG AGTTCCGGCG AGAGCGCTTC ACTGTCCACC GCACCCACCT GTACTTCCTG
CACTACGAGT ATGAGCCTGC AGCAGACAGC ACGGACGTCA CCCTGGTCGC TCAGCTGTCC
ATGGACAGGC TCCAGATGCT GGAGGCCATC TGCAAGCACT GGGAGGGGCC CATCAGCCTG
GCCCTCTACC TGTCAGACGC CGAGGCCCAG CAGTTCCTCC GCTACGCACA GGGCTCTGAG
GTGCTTATGA GCCGCCACAA CGTGGGCTAC CACATCGTGT ACAAGGAGGG CCAGTTCTAC
CCCGTGAACC TGCTGCGCAA CGTGGCCATG AAGCACATCA GCACTCCCTA CATGTTCCTG
TCTGACATTG ACTTCCTGCC CATGTATGGG CTCTATGAGT ACCTCAGGAA GTCTGTCATC
CAGCTCGATC TTGCCAACAC CAAGAAAGCA ATGATTGTCC CCGCGTTCGA GACACTGCGC
TACCGGCTGT CCTTCCCCAA GTCAAAAGCG GAGTTGCTGT CAATGCTGGA CATGGGGACC
CTCTTCACAT TCAGGTACCA CGTCTGGACG AAAGGCCACG CACCCACAAA CTTCGCCAAG
TGGCGGACCG CCACCACGCC TTACCGGGTT GAGTGGGAGG CCGATTTTGA GCCGTATGTT
GTTGTGAGAC GTGACTGCCC GGAGTACGAC CGGAGGTTTG TAGGCTTTGG CTGGAACAAA
GTGGCTCATA TCATGGAGCT GGATGTGCAG GAGTATGAGT TCATTGTGCT GCCCAACGCC
TACATGATCC ACATGCCTCA TGCCCCCAGC TTCGACATTA CCAAGTTCCG TTCCAACAAG
CAATACCGCA TCTGTCTCAA AACCCTCAAG GAAGAGTTTC AGCAGGACAT GTCCCGCCGC
TACGGCTTTG CTGCCCTGAA ATATCTCACA GCCGAGAACA ACAGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528814.1
CDS107..1774
Misc Feature(1)119..754(+)
Misc Feature(2)920..1732(+)
Translation

Target ORF information:

RefSeq Version XM_011530512
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530512

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTTAATAT GGTCTGAAGT TTCCTGGATC CCCAATAAAC ATTACTCTGG GATTTATGGT 
CTGATGAAGC TTGTCCTGAC CAAGACTCTT CCTGCCAACC TGGAGAGAGT CATCGTCCTT
GACACGGATA TCACCTTTGC CACTGACATT GCAGAGCTGT GGGCTGTGTT CCACAAGTTC
AAAGGTCAGC AAGTCCTGGG CTTGGTGGAG AACCAGAGTG ACTGGTACCT TGGAAACCTG
TGGAAAAATC ACCGCCCATG GCCAGCCCTT GGAAGAGGCT ACAACACAGG GGTGATCCTG
TTACTTCTGG ATAAGCTGCG GAAGATGAAA TGGGAGCAGA TGTGGAGGCT GACCGCAGAG
AGGGAGCTCA TGGGCATGCT CTCTACATCC TTAGCTGACC AGGATATTTT CAATGCCGTC
ATCAAACAAA ACCCCTTCCT TGTGTACCAG CTCCCCTGCT TCTGGAATGT GCAGCTGTCA
GACCACACCC GCTCCGAGCA GTGCTACAGA GACGTGTCTG ATCTAAAGGT CATTCACTGG
AACTCCCCCA AGAAGCTCCG GGTGAAGAAC AAGCATGTGG AGTTTTTTCG CAACCTCTAC
CTGACCTTCC TGGAGTATGA CGGCAATCTT CTGAGGCGGG AACTGTTTGG CTGCCCCAGT
GAGGCTGATG TCAACAGTGA AAACCTCCAG AAGCAGCTGT CTGAGCTGGA CGAGGACGAC
CTGTGCTATG AGTTCCGGCG AGAGCGCTTC ACTGTCCACC GCACCCACCT GTACTTCCTG
CACTACGAGT ATGAGCCTGC AGCAGACAGC ACGGACGTCA CCCTGGTCGC TCAGCTGTCC
ATGGACAGGC TCCAGATGCT GGAGGCCATC TGCAAGCACT GGGAGGGGCC CATCAGCCTG
GCCCTCTACC TGTCAGACGC CGAGGCCCAG CAGTTCCTCC GCTACGCACA GGGCTCTGAG
GTGCTTATGA GCCGCCACAA CGTGGGCTAC CACATCGTGT ACAAGGAGGG CCAGTTCTAC
CCCGTGAACC TGCTGCGCAA CGTGGCCATG AAGCACATCA GCACTCCCTA CATGTTCCTG
TCTGACATTG ACTTCCTGCC CATGTATGGG CTCTATGAGT ACCTCAGGAA GTCTGTCATC
CAGCTCGATC TTGCCAACAC CAAGAAAGCA ATGATTGTCC CCGCGTTCGA GACACTGCGC
TACCGGCTGT CCTTCCCCAA GTCAAAAGCG GAGTTGCTGT CAATGCTGGA CATGGGGACC
CTCTTCACAT TCAGGTACCA CGTCTGGACG AAAGGCCACG CACCCACAAA CTTCGCCAAG
TGGCGGACCG CCACCACGCC TTACCGGGTT GAGTGGGAGG CCGATTTTGA GCCGTATGTT
GTTGTGAGAC GTGACTGCCC GGAGTACGAC CGGAGGTTTG TAGGCTTTGG CTGGAACAAA
GTGGCTCATA TCATGGAGCT GGATGTGCAG GAGTATGAGT TCATTGTGCT GCCCAACGCC
TACATGATCC ACATGCCTCA TGCCCCCAGC TTCGACATTA CCAAGTTCCG TTCCAACAAG
CAATACCGCA TCTGTCTCAA AACCCTCAAG GAAGAGTTTC AGCAGGACAT GTCCCGCCGC
TACGGCTTTG CTGCCCTGAA ATATCTCACA GCCGAGAACA ACAGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62628
Clone ID Related Accession (Same CDS sequence) XM_011530513
Accession Version XM_011530513.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1173bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glycosyltransferase-like protein LARGE1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACCTCAC TTAATCTTCC CAAGTCTCAC AAGGTCATTC ACTGGAACTC CCCCAAGAAG 
CTCCGGGTGA AGAACAAGCA TGTGGAGTTT TTTCGCAACC TCTACCTGAC CTTCCTGGAG
TATGACGGCA ATCTTCTGAG GCGGGAACTG TTTGGCTGCC CCAGTGAGGC TGATGTCAAC
AGTGAAAACC TCCAGAAGCA GCTGTCTGAG CTGGACGAGG ACGACCTGTG CTATGAGTTC
CGGCGAGAGC GCTTCACTGT CCACCGCACC CACCTGTACT TCCTGCACTA CGAGTATGAG
CCTGCAGCAG ACAGCACGGA CGTCACCCTG GTCGCTCAGC TGTCCATGGA CAGGCTCCAG
ATGCTGGAGG CCATCTGCAA GCACTGGGAG GGGCCCATCA GCCTGGCCCT CTACCTGTCA
GACGCCGAGG CCCAGCAGTT CCTCCGCTAC GCACAGGGCT CTGAGGTGCT TATGAGCCGC
CACAACGTGG GCTACCACAT CGTGTACAAG GAGGGCCAGT TCTACCCCGT GAACCTGCTG
CGCAACGTGG CCATGAAGCA CATCAGCACT CCCTACATGT TCCTGTCTGA CATTGACTTC
CTGCCCATGT ATGGGCTCTA TGAGTACCTC AGGAAGTCTG TCATCCAGCT CGATCTTGCC
AACACCAAGA AAGCAATGAT TGTCCCCGCG TTCGAGACAC TGCGCTACCG GCTGTCCTTC
CCCAAGTCAA AAGCGGAGTT GCTGTCAATG CTGGACATGG GGACCCTCTT CACATTCAGG
TACCACGTCT GGACGAAAGG CCACGCACCC ACAAACTTCG CCAAGTGGCG GACCGCCACC
ACGCCTTACC GGGTTGAGTG GGAGGCCGAT TTTGAGCCGT ATGTTGTTGT GAGACGTGAC
TGCCCGGAGT ACGACCGGAG GTTTGTAGGC TTTGGCTGGA ACAAAGTGGC TCATATCATG
GAGCTGGATG TGCAGGAGTA TGAGTTCATT GTGCTGCCCA ACGCCTACAT GATCCACATG
CCTCATGCCC CCAGCTTCGA CATTACCAAG TTCCGTTCCA ACAAGCAATA CCGCATCTGT
CTCAAAACCC TCAAGGAAGA GTTTCAGCAG GACATGTCCC GCCGCTACGG CTTTGCTGCC
CTGAAATATC TCACAGCCGA GAACAACAGC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528815.1
CDS64..1236
Misc Feature(1)94..216(+)
Misc Feature(2)382..1194(+)
Translation

Target ORF information:

RefSeq Version XM_011530513
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530513

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACCTCAC TTAATCTTCC CAAGTCTCAC AAGGTCATTC ACTGGAACTC CCCCAAGAAG 
CTCCGGGTGA AGAACAAGCA TGTGGAGTTT TTTCGCAACC TCTACCTGAC CTTCCTGGAG
TATGACGGCA ATCTTCTGAG GCGGGAACTG TTTGGCTGCC CCAGTGAGGC TGATGTCAAC
AGTGAAAACC TCCAGAAGCA GCTGTCTGAG CTGGACGAGG ACGACCTGTG CTATGAGTTC
CGGCGAGAGC GCTTCACTGT CCACCGCACC CACCTGTACT TCCTGCACTA CGAGTATGAG
CCTGCAGCAG ACAGCACGGA CGTCACCCTG GTCGCTCAGC TGTCCATGGA CAGGCTCCAG
ATGCTGGAGG CCATCTGCAA GCACTGGGAG GGGCCCATCA GCCTGGCCCT CTACCTGTCA
GACGCCGAGG CCCAGCAGTT CCTCCGCTAC GCACAGGGCT CTGAGGTGCT TATGAGCCGC
CACAACGTGG GCTACCACAT CGTGTACAAG GAGGGCCAGT TCTACCCCGT GAACCTGCTG
CGCAACGTGG CCATGAAGCA CATCAGCACT CCCTACATGT TCCTGTCTGA CATTGACTTC
CTGCCCATGT ATGGGCTCTA TGAGTACCTC AGGAAGTCTG TCATCCAGCT CGATCTTGCC
AACACCAAGA AAGCAATGAT TGTCCCCGCG TTCGAGACAC TGCGCTACCG GCTGTCCTTC
CCCAAGTCAA AAGCGGAGTT GCTGTCAATG CTGGACATGG GGACCCTCTT CACATTCAGG
TACCACGTCT GGACGAAAGG CCACGCACCC ACAAACTTCG CCAAGTGGCG GACCGCCACC
ACGCCTTACC GGGTTGAGTG GGAGGCCGAT TTTGAGCCGT ATGTTGTTGT GAGACGTGAC
TGCCCGGAGT ACGACCGGAG GTTTGTAGGC TTTGGCTGGA ACAAAGTGGC TCATATCATG
GAGCTGGATG TGCAGGAGTA TGAGTTCATT GTGCTGCCCA ACGCCTACAT GATCCACATG
CCTCATGCCC CCAGCTTCGA CATTACCAAG TTCCGTTCCA ACAAGCAATA CCGCATCTGT
CTCAAAACCC TCAAGGAAGA GTTTCAGCAG GACATGTCCC GCCGCTACGG CTTTGCTGCC
CTGAAATATC TCACAGCCGA GAACAACAGC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62629
Clone ID Related Accession (Same CDS sequence) XM_011530514
Accession Version XM_011530514.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1152bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glycosyltransferase-like protein LARGE1 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GACAGTGGTT
GACCTCTGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528816.1
CDS628..1779
Misc Feature(1)1033..>1704(+)
Misc Feature(2)1039..1764(+)
Misc Feature(3)1054..1710(+)
Misc Feature(4)1351..1359(+)
Translation

Target ORF information:

RefSeq Version XM_011530514
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens like-glycosyltransferase (LARGE), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530514

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GACAGTGGTT
GACCTCTGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28267
Clone ID Related Accession (Same CDS sequence) NM_004737 , NM_133642 , XM_005261831 , XM_005261832 , XM_011530510
Accession Version NM_004737.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glycosyltransferase-like protein LARGE1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR000293.1, AI198348.1, AJ007583.1, BF057493.1 and BC117425.1. This sequence is a reference standard in the RefSeqGene project. On May 24, 2008 this sequence version replaced gi:59853527. Transcript Variant: This variant (1) represents the longer transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ007583.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GGTCATTCAC
TGGAACTCCC CCAAGAAGCT CCGGGTGAAG AACAAGCATG TGGAGTTTTT TCGCAACCTC
TACCTGACCT TCCTGGAGTA TGACGGCAAT CTTCTGAGGC GGGAACTGTT TGGCTGCCCC
AGTGAGGCTG ATGTCAACAG TGAAAACCTC CAGAAGCAGC TGTCTGAGCT GGACGAGGAC
GACCTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACCGCACCCA CCTGTACTTC
CTGCACTACG AGTATGAGCC TGCAGCAGAC AGCACGGACG TCACCCTGGT CGCTCAGCTG
TCCATGGACA GGCTCCAGAT GCTGGAGGCC ATCTGCAAGC ACTGGGAGGG GCCCATCAGC
CTGGCCCTCT ACCTGTCAGA CGCCGAGGCC CAGCAGTTCC TCCGCTACGC ACAGGGCTCT
GAGGTGCTTA TGAGCCGCCA CAACGTGGGC TACCACATCG TGTACAAGGA GGGCCAGTTC
TACCCCGTGA ACCTGCTGCG CAACGTGGCC ATGAAGCACA TCAGCACTCC CTACATGTTC
CTGTCTGACA TTGACTTCCT GCCCATGTAT GGGCTCTATG AGTACCTCAG GAAGTCTGTC
ATCCAGCTCG ATCTTGCCAA CACCAAGAAA GCAATGATTG TCCCCGCGTT CGAGACACTG
CGCTACCGGC TGTCCTTCCC CAAGTCAAAA GCGGAGTTGC TGTCAATGCT GGACATGGGG
ACCCTCTTCA CATTCAGGTA CCACGTCTGG ACGAAAGGCC ACGCACCCAC AAACTTCGCC
AAGTGGCGGA CCGCCACCAC GCCTTACCGG GTTGAGTGGG AGGCCGATTT TGAGCCGTAT
GTTGTTGTGA GACGTGACTG CCCGGAGTAC GACCGGAGGT TTGTAGGCTT TGGCTGGAAC
AAAGTGGCTC ATATCATGGA GCTGGATGTG CAGGAGTATG AGTTCATTGT GCTGCCCAAC
GCCTACATGA TCCACATGCC TCATGCCCCC AGCTTCGACA TTACCAAGTT CCGTTCCAAC
AAGCAATACC GCATCTGTCT CAAAACCCTC AAGGAAGAGT TTCAGCAGGA CATGTCCCGC
CGCTACGGCT TTGCTGCCCT GAAATATCTC ACAGCCGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004728.1
CDS580..2850
Misc Feature(1)490..492(+)
Misc Feature(2)610..672(+)
Misc Feature(3)985..>1737(+)
Misc Feature(4)991..1830(+)
Misc Feature(5)1006..1737(+)
Misc Feature(6)1303..1719(+)
Misc Feature(7)1996..2808(+)
Exon (1)1..434
Gene:LARGE
Gene Synonym:
Exon (2)435..497
Gene:LARGE
Gene Synonym:
Exon (3)498..685
Gene:LARGE
Gene Synonym:
Exon (4)686..987
Gene:LARGE
Gene Synonym:
Exon (5)988..1070
Gene:LARGE
Gene Synonym:
Exon (6)1071..1194
Gene:LARGE
Gene Synonym:
Exon (7)1195..1366
Gene:LARGE
Gene Synonym:
Exon (8)1367..1471
Gene:LARGE
Gene Synonym:
Exon (9)1472..1584
Gene:LARGE
Gene Synonym:
Exon (10)1585..1710
Gene:LARGE
Gene Synonym:
Exon (11)1711..1866
Gene:LARGE
Gene Synonym:
Exon (12)1867..2030
Gene:LARGE
Gene Synonym:
Exon (13)2031..2309
Gene:LARGE
Gene Synonym:
Exon (14)2310..2456
Gene:LARGE
Gene Synonym:
Exon (15)2457..2652
Gene:LARGE
Gene Synonym:
Exon (16)2653..4201
Gene:LARGE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004737
Organism Homo sapiens (human)
Definition Homo sapiens like-glycosyltransferase (LARGE), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004737

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GGTCATTCAC
TGGAACTCCC CCAAGAAGCT CCGGGTGAAG AACAAGCATG TGGAGTTTTT TCGCAACCTC
TACCTGACCT TCCTGGAGTA TGACGGCAAT CTTCTGAGGC GGGAACTGTT TGGCTGCCCC
AGTGAGGCTG ATGTCAACAG TGAAAACCTC CAGAAGCAGC TGTCTGAGCT GGACGAGGAC
GACCTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACCGCACCCA CCTGTACTTC
CTGCACTACG AGTATGAGCC TGCAGCAGAC AGCACGGACG TCACCCTGGT CGCTCAGCTG
TCCATGGACA GGCTCCAGAT GCTGGAGGCC ATCTGCAAGC ACTGGGAGGG GCCCATCAGC
CTGGCCCTCT ACCTGTCAGA CGCCGAGGCC CAGCAGTTCC TCCGCTACGC ACAGGGCTCT
GAGGTGCTTA TGAGCCGCCA CAACGTGGGC TACCACATCG TGTACAAGGA GGGCCAGTTC
TACCCCGTGA ACCTGCTGCG CAACGTGGCC ATGAAGCACA TCAGCACTCC CTACATGTTC
CTGTCTGACA TTGACTTCCT GCCCATGTAT GGGCTCTATG AGTACCTCAG GAAGTCTGTC
ATCCAGCTCG ATCTTGCCAA CACCAAGAAA GCAATGATTG TCCCCGCGTT CGAGACACTG
CGCTACCGGC TGTCCTTCCC CAAGTCAAAA GCGGAGTTGC TGTCAATGCT GGACATGGGG
ACCCTCTTCA CATTCAGGTA CCACGTCTGG ACGAAAGGCC ACGCACCCAC AAACTTCGCC
AAGTGGCGGA CCGCCACCAC GCCTTACCGG GTTGAGTGGG AGGCCGATTT TGAGCCGTAT
GTTGTTGTGA GACGTGACTG CCCGGAGTAC GACCGGAGGT TTGTAGGCTT TGGCTGGAAC
AAAGTGGCTC ATATCATGGA GCTGGATGTG CAGGAGTATG AGTTCATTGT GCTGCCCAAC
GCCTACATGA TCCACATGCC TCATGCCCCC AGCTTCGACA TTACCAAGTT CCGTTCCAAC
AAGCAATACC GCATCTGTCT CAAAACCCTC AAGGAAGAGT TTCAGCAGGA CATGTCCCGC
CGCTACGGCT TTGCTGCCCT GAAATATCTC ACAGCCGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28267
Clone ID Related Accession (Same CDS sequence) NM_004737 , NM_133642 , XM_005261831 , XM_005261832 , XM_011530510
Accession Version NM_133642.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glycosyltransferase-like protein LARGE1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR000293.1, AI198348.1, AJ007583.1, BF057493.1 and BC117425.1. On May 24, 2008 this sequence version replaced gi:59853581. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AJ007583.1, BC117425.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GGTCATTCAC
TGGAACTCCC CCAAGAAGCT CCGGGTGAAG AACAAGCATG TGGAGTTTTT TCGCAACCTC
TACCTGACCT TCCTGGAGTA TGACGGCAAT CTTCTGAGGC GGGAACTGTT TGGCTGCCCC
AGTGAGGCTG ATGTCAACAG TGAAAACCTC CAGAAGCAGC TGTCTGAGCT GGACGAGGAC
GACCTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACCGCACCCA CCTGTACTTC
CTGCACTACG AGTATGAGCC TGCAGCAGAC AGCACGGACG TCACCCTGGT CGCTCAGCTG
TCCATGGACA GGCTCCAGAT GCTGGAGGCC ATCTGCAAGC ACTGGGAGGG GCCCATCAGC
CTGGCCCTCT ACCTGTCAGA CGCCGAGGCC CAGCAGTTCC TCCGCTACGC ACAGGGCTCT
GAGGTGCTTA TGAGCCGCCA CAACGTGGGC TACCACATCG TGTACAAGGA GGGCCAGTTC
TACCCCGTGA ACCTGCTGCG CAACGTGGCC ATGAAGCACA TCAGCACTCC CTACATGTTC
CTGTCTGACA TTGACTTCCT GCCCATGTAT GGGCTCTATG AGTACCTCAG GAAGTCTGTC
ATCCAGCTCG ATCTTGCCAA CACCAAGAAA GCAATGATTG TCCCCGCGTT CGAGACACTG
CGCTACCGGC TGTCCTTCCC CAAGTCAAAA GCGGAGTTGC TGTCAATGCT GGACATGGGG
ACCCTCTTCA CATTCAGGTA CCACGTCTGG ACGAAAGGCC ACGCACCCAC AAACTTCGCC
AAGTGGCGGA CCGCCACCAC GCCTTACCGG GTTGAGTGGG AGGCCGATTT TGAGCCGTAT
GTTGTTGTGA GACGTGACTG CCCGGAGTAC GACCGGAGGT TTGTAGGCTT TGGCTGGAAC
AAAGTGGCTC ATATCATGGA GCTGGATGTG CAGGAGTATG AGTTCATTGT GCTGCCCAAC
GCCTACATGA TCCACATGCC TCATGCCCCC AGCTTCGACA TTACCAAGTT CCGTTCCAAC
AAGCAATACC GCATCTGTCT CAAAACCCTC AAGGAAGAGT TTCAGCAGGA CATGTCCCGC
CGCTACGGCT TTGCTGCCCT GAAATATCTC ACAGCCGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_598397.1
CDS517..2787
Misc Feature(1)319..321(+)
Misc Feature(2)547..609(+)
Misc Feature(3)922..>1674(+)
Misc Feature(4)928..1767(+)
Misc Feature(5)943..1674(+)
Misc Feature(6)1240..1656(+)
Misc Feature(7)1933..2745(+)
Exon (1)1..434
Gene:LARGE
Gene Synonym:
Exon (2)435..622
Gene:LARGE
Gene Synonym:
Exon (3)623..924
Gene:LARGE
Gene Synonym:
Exon (4)925..1007
Gene:LARGE
Gene Synonym:
Exon (5)1008..1131
Gene:LARGE
Gene Synonym:
Exon (6)1132..1303
Gene:LARGE
Gene Synonym:
Exon (7)1304..1408
Gene:LARGE
Gene Synonym:
Exon (8)1409..1521
Gene:LARGE
Gene Synonym:
Exon (9)1522..1647
Gene:LARGE
Gene Synonym:
Exon (10)1648..1803
Gene:LARGE
Gene Synonym:
Exon (11)1804..1967
Gene:LARGE
Gene Synonym:
Exon (12)1968..2246
Gene:LARGE
Gene Synonym:
Exon (13)2247..2393
Gene:LARGE
Gene Synonym:
Exon (14)2394..2589
Gene:LARGE
Gene Synonym:
Exon (15)2590..4138
Gene:LARGE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_133642
Organism Homo sapiens (human)
Definition Homo sapiens like-glycosyltransferase (LARGE), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_133642

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTGGGAA TCTGCAGGGG GAGACGGAAA TTCTTGGCTG CCTCGTTGAG TCTTCTCTGC 
ATCCCAGCCA TCACCTGGAT TTACCTGTTT TCTGGGAGCT TCGAAGATGG AAAGCCCGTG
TCTCTGTCAC CGCTGGAGTC CCAGGCACAC AGCCCCAGGT ACACGGCCTC CAGCCAGCGG
GAGCGCGAGA GCCTGGAGGT GCGCATGCGC GAGGTGGAGG AGGAGAACCG CGCCCTCCGC
AGGCAGCTCA GCCTGGCCCA GGGCCGAGCC CCATCCCATC GCCGAGGCAA CCACTCCAAG
ACCTACTCCA TGGAGGAGGG CACTGGAGAC AGCGAGAACC TTCGGGCTGG CATCGTGGCA
GGCAACAGCT CCGAGTGTGG GCAGCAGCCG GTCGTGGAGA AATGCGAGAC AATCCACGTT
GCTATTGTCT GCGCCGGATA CAATGCCAGC CGGGATGTCG TCACCCTGGT CAAATCCGTC
CTGTTCCATA GACGGAACCC TCTGCACTTC CACCTTATTG CTGACTCCAT TGCGGAGCAG
ATCCTGGCCA CGCTCTTCCA GACCTGGATG GTGCCCGCTG TGCGTGTGGA CTTCTACAAT
GCAGACGAGC TCAAGTCTGA AGTTTCCTGG ATCCCCAATA AACATTACTC TGGGATTTAT
GGTCTGATGA AGCTTGTCCT GACCAAGACT CTTCCTGCCA ACCTGGAGAG AGTCATCGTC
CTTGACACGG ATATCACCTT TGCCACTGAC ATTGCAGAGC TGTGGGCTGT GTTCCACAAG
TTCAAAGGTC AGCAAGTCCT GGGCTTGGTG GAGAACCAGA GTGACTGGTA CCTTGGAAAC
CTGTGGAAAA ATCACCGCCC ATGGCCAGCC CTTGGAAGAG GCTACAACAC AGGGGTGATC
CTGTTACTTC TGGATAAGCT GCGGAAGATG AAATGGGAGC AGATGTGGAG GCTGACCGCA
GAGAGGGAGC TCATGGGCAT GCTCTCTACA TCCTTAGCTG ACCAGGATAT TTTCAATGCC
GTCATCAAAC AAAACCCCTT CCTTGTGTAC CAGCTCCCCT GCTTCTGGAA TGTGCAGCTG
TCAGACCACA CCCGCTCCGA GCAGTGCTAC AGAGACGTGT CTGATCTAAA GGTCATTCAC
TGGAACTCCC CCAAGAAGCT CCGGGTGAAG AACAAGCATG TGGAGTTTTT TCGCAACCTC
TACCTGACCT TCCTGGAGTA TGACGGCAAT CTTCTGAGGC GGGAACTGTT TGGCTGCCCC
AGTGAGGCTG ATGTCAACAG TGAAAACCTC CAGAAGCAGC TGTCTGAGCT GGACGAGGAC
GACCTGTGCT ATGAGTTCCG GCGAGAGCGC TTCACTGTCC ACCGCACCCA CCTGTACTTC
CTGCACTACG AGTATGAGCC TGCAGCAGAC AGCACGGACG TCACCCTGGT CGCTCAGCTG
TCCATGGACA GGCTCCAGAT GCTGGAGGCC ATCTGCAAGC ACTGGGAGGG GCCCATCAGC
CTGGCCCTCT ACCTGTCAGA CGCCGAGGCC CAGCAGTTCC TCCGCTACGC ACAGGGCTCT
GAGGTGCTTA TGAGCCGCCA CAACGTGGGC TACCACATCG TGTACAAGGA GGGCCAGTTC
TACCCCGTGA ACCTGCTGCG CAACGTGGCC ATGAAGCACA TCAGCACTCC CTACATGTTC
CTGTCTGACA TTGACTTCCT GCCCATGTAT GGGCTCTATG AGTACCTCAG GAAGTCTGTC
ATCCAGCTCG ATCTTGCCAA CACCAAGAAA GCAATGATTG TCCCCGCGTT CGAGACACTG
CGCTACCGGC TGTCCTTCCC CAAGTCAAAA GCGGAGTTGC TGTCAATGCT GGACATGGGG
ACCCTCTTCA CATTCAGGTA CCACGTCTGG ACGAAAGGCC ACGCACCCAC AAACTTCGCC
AAGTGGCGGA CCGCCACCAC GCCTTACCGG GTTGAGTGGG AGGCCGATTT TGAGCCGTAT
GTTGTTGTGA GACGTGACTG CCCGGAGTAC GACCGGAGGT TTGTAGGCTT TGGCTGGAAC
AAAGTGGCTC ATATCATGGA GCTGGATGTG CAGGAGTATG AGTTCATTGT GCTGCCCAAC
GCCTACATGA TCCACATGCC TCATGCCCCC AGCTTCGACA TTACCAAGTT CCGTTCCAAC
AAGCAATACC GCATCTGTCT CAAAACCCTC AAGGAAGAGT TTCAGCAGGA CATGTCCCGC
CGCTACGGCT TTGCTGCCCT GAAATATCTC ACAGCCGAGA ACAACAGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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