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MAGI1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAGI1
Entrez Gene ID 9223
Full Name membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms AIP-3, AIP3, BAIAP1, BAP-1, BAP1, MAGI-1, Magi1d, TNRC19, WWP3
General protein information
Preferred Names
membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
Names
membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
BAI1-associated protein 1
WW domain-containing protein 3
atrophin-1-interacting protein 3
membrane-associated guanylate kinase inverted 1
trinucleotide repeat-containing gene 19 protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3p14.1

Summary The protein encoded by this gene is a member of the membrane-associated guanylate kinase homologue (MAGUK) family. MAGUK proteins participate in the assembly of multiprotein complexes on the inner surface of the plasma membrane at regions of cell-cell contact. The product of this gene may play a role as scaffolding protein at cell-cell junctions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

602625


mRNA and Protein(s)

mRNA Protein Name
XM_006713407 XP_006713470 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
XM_006713408 XP_006713471 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
XM_006713409 XP_006713472 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
XM_005265563 XP_005265620 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
XM_005265564 XP_005265621 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
XM_005265565 XP_005265622 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X6
XM_011534236 XP_011532538 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X7
XM_005265566 XP_005265623 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X8
XM_006713410 XP_006713473 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X9
XM_005265568 XP_005265625 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X10
XM_011534237 XP_011532539 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X11
XM_006713411 XP_006713474 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X12
XM_006713412 XP_006713475 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X13
XM_005265570 XP_005265627 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X14
XM_005265571 XP_005265628 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X15
XM_006713413 XP_006713476 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X16
XM_006713414 XP_006713477 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X17
XM_005265574 XP_005265631 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X18
XM_011534238 XP_011532540 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X19
XM_011534239 XP_011532541 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X20
XM_011534240 XP_011532542 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X21
XM_011534241 XP_011532543 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X22
NM_004742 NP_004733 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform b
NM_015520 NP_056335 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform a
NM_001033057 NP_001028229 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform c



Homo sapiens (human) MAGI1 NP_001028229.1
Pan troglodytes (chimpanzee) MAGI1 XP_003309903.1
Canis lupus familiaris (dog) MAGI1 XP_005633237.1
Bos taurus (cattle) MAGI1 XP_002697009.1
Mus musculus (house mouse) Magi1 NP_001025021.1
Rattus norvegicus (Norway rat) Magi1 NP_001025216.1
Gallus gallus (chicken) MAGI1 XP_003642069.1
Danio rerio (zebrafish) magi1b NP_001007064.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAGI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu37583 XM_006713407 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37584 XM_006713408 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37585 XM_006713409 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37586 XM_005265563 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37587 XM_005265564 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37588 XM_005265565 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu62631 XM_011534236 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37589 XM_005265566 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37590 XM_006713410 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37591 XM_005265568 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu62632 XM_011534237 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37592 XM_006713411 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37593 XM_006713412 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37594 XM_005265570 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37595 XM_005265571 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu62633 XM_006713413 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X16, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu37597 XM_006713414 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X17, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu37598 XM_005265574 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X18, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu62634 XM_011534238 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X19, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu62635 XM_011534239 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X20, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu62636 XM_011534240 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X21, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $391.30
$559.00
OHu62637 XM_011534241 PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X22, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $391.30
$559.00
OHu24845 NM_004742 Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $888.30
$1269.00
OHu24816
NM_015520 Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$649.50-$909.30
$1299.00
OHu24988 NM_001033057 Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $923.30
$1319.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu37583
Clone ID Related Accession (Same CDS sequence) XM_006713407
Accession Version XM_006713407.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACCTATGTCA CCTTCACCTG CGTCGGGATT GAGCAAGGGT
GAGAGAGAGA GAGAAATCAA TTCCACGAAT TTTGGAGAAT GTCCGATTCC AGATTACCAG
GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACTGGATTTG GATTTAGGAT TCTGGGTGGA
AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACT
GACGGCCGCC TGAGGTCTGG AGATGAATTA ATCTGTGTGG ATGGGACGCC AGTAATTGGA
AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCTG CCAAGCAAGG CCACGTCAAT
CTCACGGTGC GGCGTAAAGT GGTTTTTGCG GTGCCCAAAA CCGAGAACGA GGTGCCCTCG
CCAGCCTCCT CTCATCACAG TAGCAACCAG CCGGCCTCGC TGACAGAAGA GAAGCGCACT
CCGCAGGGCA GCCAGAACTC GCTGAACACG GTGAGCTCGG GCAGCGGCAG CACCAGCGGC
ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC GTGGTCAGCA CCGTGGTGCA GCCCTACGAC
GTGGAGATCC GGCGCGGGGA GAACGAGGGC TTCGGCTTCG TCATCGTGTC CTCGGTGAGC
AGGCCCGAAG CAGGCACGAC CTTTGCAGGC AATGCATGTG TGGCTATGCC TCACAAAATA
GGTCGGATTA TTGAGGGGAG CCCTGCTGAC CGCTGTGGCA AGCTGAAAGT AGGAGACCGG
ATCTTGGCAG TAAATGGATG TTCCATCACC AACAAATCCC ATTCAGACAT TGTGAACCTA
ATCAAGGAAG CGGGAAACAC AGTTACCCTC CGCATCATTC CTGGGGATGA GTCCTCGAAT
GCCACCTTGC TGACCAATGC AGAGAAGATT GCCACCATCA CCACCACACA CACCCCTTCT
CAGCAAGGGA CCCAGGAGAC AAGGAATACC ACCAAACCAA AGCAGGAATC TCAATTTGAG
TTCAAAGCAC CCCAAGCAAC ACAGGAGCAA GATTTTTACA CTGTGGAACT GGAAAGAGGA
GCCAAGGGAT TTGGCTTTAG CCTTCGAGGA GGCCGAGAGT ATAACATGGA CCTCTATGTT
CTGCGCTTAG CAGAGGACGG TCCTGCGGAG AGGTGTGGAA AGATGAGGAT TGGTGATGAA
ATTTTAGAGA TCAATGGTGA GACCACCAAA AACATGAAGC ATTCTCGAGC TATAGAACTG
ATTAAGAATG GTGGCCGCAG AGTTCGTCTG TTTCTGAAGC GGGGAGACGG CTCAGTACCA
GAATATGACC CCAGCAGCGA CCGCCACGGC CCCGCCACCG GTCCACAAGG TGTTCCGGAA
GTGAGGGCCG GGCCCGACCG CCGGCAGCAT CCGTCATTGG AGTCCAGTTA CCCACCCGAT
CTTCACAAAT CATCACCACA TGGGGAAAAG CGGGCACACG CAAGGGATCC GAAAGGCAGC
AGAGAGTATA GCAGACAACC CAATGAACAC CACACCTGGA ATGGGACTTC GAGGAAACCC
GACAGCGGGG CATGCCGACC CAAGGACCGG GCGCCGGAGG GACGGAGGGA CGCGCAGGCC
GAGCGCGCGG CAGCCGCCAA CGGCCCCAAG AGGCGGTCCC CAGAGAAGCG GAGGGAGGGC
ACCCGCAGCG CCGACAACAC TTTGGAGAGG AGGGAGAAGC ACGAGAAGAG ACGAGACGTC
TCTCCGGAGC GGAGGCGAGA GCGGTCACCC ACCCGCAGGA GAGACGGCTC CCCCAGCCGG
CGGAGACGGT CTCTGGAGAG ACTCCTGGAG CAGAGGAGGT CCCCCGAGCG CAGGAGAGGG
GGCTCGCCCG AGCGCAGGGC CAAGTCCACC GACCGGAGGC GCGCACGCTC CCCCGAGCGC
AGGAGAGAGC GGTCCCTGGA CAAAAGGAAC AGAGAGGACA GAGCCAGCCA CCGAGAAAGG
GAAGAGGCGA ATCTGAAACA GGATGCCGGC AGAAGTTCCA GACATCCCCC GGAGCAGAGA
AGGCGACCTT ACAAAGAATG TAGCACCGAC CTCAGTATCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713470.1
CDS529..5010
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1410(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1438..1527(+)
Misc Feature(6)1480..1515(+)
Misc Feature(7)1618..1710(+)
Misc Feature(8)1660..1695(+)
Misc Feature(9)1960..2163(+)
Misc Feature(10)1975..2154(+)
Misc Feature(11)2455..2694(+)
Misc Feature(12)2488..2655(+)
Misc Feature(13)3073..3300(+)
Misc Feature(14)3082..3261(+)
Misc Feature(15)3520..3807(+)
Misc Feature(16)3556..3774(+)
Misc Feature(17)3985..4218(+)
Misc Feature(18)4015..4194(+)
Translation

Target ORF information:

RefSeq Version XM_006713407
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006713407

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACCTATGTCA CCTTCACCTG CGTCGGGATT GAGCAAGGGT
GAGAGAGAGA GAGAAATCAA TTCCACGAAT TTTGGAGAAT GTCCGATTCC AGATTACCAG
GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACTGGATTTG GATTTAGGAT TCTGGGTGGA
AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACT
GACGGCCGCC TGAGGTCTGG AGATGAATTA ATCTGTGTGG ATGGGACGCC AGTAATTGGA
AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCTG CCAAGCAAGG CCACGTCAAT
CTCACGGTGC GGCGTAAAGT GGTTTTTGCG GTGCCCAAAA CCGAGAACGA GGTGCCCTCG
CCAGCCTCCT CTCATCACAG TAGCAACCAG CCGGCCTCGC TGACAGAAGA GAAGCGCACT
CCGCAGGGCA GCCAGAACTC GCTGAACACG GTGAGCTCGG GCAGCGGCAG CACCAGCGGC
ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC GTGGTCAGCA CCGTGGTGCA GCCCTACGAC
GTGGAGATCC GGCGCGGGGA GAACGAGGGC TTCGGCTTCG TCATCGTGTC CTCGGTGAGC
AGGCCCGAAG CAGGCACGAC CTTTGCAGGC AATGCATGTG TGGCTATGCC TCACAAAATA
GGTCGGATTA TTGAGGGGAG CCCTGCTGAC CGCTGTGGCA AGCTGAAAGT AGGAGACCGG
ATCTTGGCAG TAAATGGATG TTCCATCACC AACAAATCCC ATTCAGACAT TGTGAACCTA
ATCAAGGAAG CGGGAAACAC AGTTACCCTC CGCATCATTC CTGGGGATGA GTCCTCGAAT
GCCACCTTGC TGACCAATGC AGAGAAGATT GCCACCATCA CCACCACACA CACCCCTTCT
CAGCAAGGGA CCCAGGAGAC AAGGAATACC ACCAAACCAA AGCAGGAATC TCAATTTGAG
TTCAAAGCAC CCCAAGCAAC ACAGGAGCAA GATTTTTACA CTGTGGAACT GGAAAGAGGA
GCCAAGGGAT TTGGCTTTAG CCTTCGAGGA GGCCGAGAGT ATAACATGGA CCTCTATGTT
CTGCGCTTAG CAGAGGACGG TCCTGCGGAG AGGTGTGGAA AGATGAGGAT TGGTGATGAA
ATTTTAGAGA TCAATGGTGA GACCACCAAA AACATGAAGC ATTCTCGAGC TATAGAACTG
ATTAAGAATG GTGGCCGCAG AGTTCGTCTG TTTCTGAAGC GGGGAGACGG CTCAGTACCA
GAATATGACC CCAGCAGCGA CCGCCACGGC CCCGCCACCG GTCCACAAGG TGTTCCGGAA
GTGAGGGCCG GGCCCGACCG CCGGCAGCAT CCGTCATTGG AGTCCAGTTA CCCACCCGAT
CTTCACAAAT CATCACCACA TGGGGAAAAG CGGGCACACG CAAGGGATCC GAAAGGCAGC
AGAGAGTATA GCAGACAACC CAATGAACAC CACACCTGGA ATGGGACTTC GAGGAAACCC
GACAGCGGGG CATGCCGACC CAAGGACCGG GCGCCGGAGG GACGGAGGGA CGCGCAGGCC
GAGCGCGCGG CAGCCGCCAA CGGCCCCAAG AGGCGGTCCC CAGAGAAGCG GAGGGAGGGC
ACCCGCAGCG CCGACAACAC TTTGGAGAGG AGGGAGAAGC ACGAGAAGAG ACGAGACGTC
TCTCCGGAGC GGAGGCGAGA GCGGTCACCC ACCCGCAGGA GAGACGGCTC CCCCAGCCGG
CGGAGACGGT CTCTGGAGAG ACTCCTGGAG CAGAGGAGGT CCCCCGAGCG CAGGAGAGGG
GGCTCGCCCG AGCGCAGGGC CAAGTCCACC GACCGGAGGC GCGCACGCTC CCCCGAGCGC
AGGAGAGAGC GGTCCCTGGA CAAAAGGAAC AGAGAGGACA GAGCCAGCCA CCGAGAAAGG
GAAGAGGCGA ATCTGAAACA GGATGCCGGC AGAAGTTCCA GACATCCCCC GGAGCAGAGA
AGGCGACCTT ACAAAGAATG TAGCACCGAC CTCAGTATCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37584
Clone ID Related Accession (Same CDS sequence) XM_006713408
Accession Version XM_006713408.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4479bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGAAGGG GTACACACCG AGGAGCTGGA CAGTGAACTA
GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
CACATCAACA GGAAGACACA ATATGAGAAC CCGGTTCTAG AAGCCAAACG GAAGAAGCAG
CTTGAGCAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
CAGCAGACAG AAGAATGGAC AGAAGATCAC TCAGCCCTTG TGCCTCCTGT TATTCCAAAC
CACCCTCCAA GCAATCCAGA GCCAGCCAGA GAAGTTCCAC TTCAGGGCAA ACCCTTTTTT
ACACGAAACC CTTCTGAGTT GAAAGGCAAG TTCATTCACA CAAAGCTGCG GAAAAGCAGT
CGTGGCTTTG GCTTCACGGT GGTTGGAGGG GATGAACCTG ATGAGTTTCT CCAGATCAAG
AGCTTGGTCC TAGATGGTCC TGCTGCATTG GATGGCAAGA TGGAAACAGG GGATGTGATT
GTAAGTGTGA ATGACACCTG TGTTTTGGGA CACACACATG CTCAAGTTGT GAAGATCTTC
CAGTCCATCC CCATTGGTGC CAGCGTGGAC CTTGAACTCT GCCGAGGTTA TCCATTGCCT
TTTGATCCAG ATGACCCCAA TACAAGTTTA GTGACCTCGG TAGCCATTTT GGATAAAGAA
CCAATTATTG TGAATGGGCA AGAGACCTAT GATTCACCAG CTAGCCACAG TAGTAAAACA
GGCAAAGTCA ATGGCATGAA GGATGCCAGG CCAAGCAGCC CAGCGGACGT GGCTTCAAAT
AGTTCTCATG GTTATCCTAA TGACACTGTT TCTTTGGCTT CCTCCATAGC CACTCAGCCA
GAACTCATAA CTGTTCATAT TGTCAAAGGG CCAATGGGCT TTGGTTTTAC TATCGCAGAC
AGTCCTGGTG GGGGTGGCCA AAGAGTGAAA CAGATTGTTG ACAGCCCAAG GTGCCGAGGC
CTGAAAGAAG GGGATCTCAT AGTGGAAGTT AATAAGAAGA ACGTGCAGGC CCTAACTCAC
AACCAAGTGG TGGATATGCT GGTTGAGTGT CCTAAGGGAA GTGAGGTCAC ATTGTTGGTG
CAACGAGGAG GGCTGCCAGT TCCCAAGAAG AGCCCAAAGT CGCAACCACT GGAAAGGAAA
GACAGCCAGA ATAGTTCTCA GCACAGTGTT TCCAGCCACC GAAGCCTGCA CACAGCATCC
CCAAGCCACA GCACACAGGT GCTCCCCGAG TTCCCACCTG CAGAGGCCCA AGCTCCAGAT
CAAACTGACA GCTCTGGCCA GAAAAAACCA GATCCTTTTA AAATCTGGGC CCAGTCCAGG
AGCATGTATG AAAACCGACC TATGTCACCT TCACCTGCGT CGGGATTGAG CAAGGGTGAG
AGAGAGAGAG AAATCAATTC CACGAATTTT GGAGAATGTC CGATTCCAGA TTACCAGGAA
CAGGACATCT TCCTCTGGAG AAAAGAGACT GGATTTGGAT TTAGGATTCT GGGTGGAAAT
GAACCAGGGG AACCTATTTA TATTGGTCAC ATCGTACCAC TGGGTGCTGC TGATACTGAC
GGCCGCCTGA GGTCTGGAGA TGAATTAATC TGTGTGGATG GGACGCCAGT AATTGGAAAA
TCACACCAGC TTGTGGTCCA GCTTATGCAA CAAGCTGCCA AGCAAGGCCA CGTCAATCTC
ACGGTGCGGC GTAAAGTGGT TTTTGCGGTG CCCAAAACCG AGAACGAGGT GCCCTCGCCA
GCCTCCTCTC ATCACAGTAG CAACCAGCCG GCCTCGCTGA CAGAAGAGAA GCGCACTCCG
CAGGGCAGCC AGAACTCGCT GAACACGGTG AGCTCGGGCA GCGGCAGCAC CAGCGGCATC
GGCAGTGGCG GCGGCGGGGG CAGCGGCGTG GTCAGCACCG TGGTGCAGCC CTACGACGTG
GAGATCCGGC GCGGGGAGAA CGAGGGCTTC GGCTTCGTCA TCGTGTCCTC GGTGAGCAGG
CCCGAAGCAG GCACGACCTT TGCAGGCAAT GCATGTGTGG CTATGCCTCA CAAAATAGGT
CGGATTATTG AGGGGAGCCC TGCTGACCGC TGTGGCAAGC TGAAAGTAGG AGACCGGATC
TTGGCAGTAA ATGGATGTTC CATCACCAAC AAATCCCATT CAGACATTGT GAACCTAATC
AAGGAAGCGG GAAACACAGT TACCCTCCGC ATCATTCCTG GGGATGAGTC CTCGAATGCC
ACCTTGCTGA CCAATGCAGA GAAGATTGCC ACCATCACCA CCACACACAC CCCTTCTCAG
CAAGGGACCC AGGAGACAAG GAATACCACC AAACCAAAGC AGGAATCTCA ATTTGAGTTC
AAAGCACCCC AAGCAACACA GGAGCAAGAT TTTTACACTG TGGAACTGGA AAGAGGAGCC
AAGGGATTTG GCTTTAGCCT TCGAGGAGGC CGAGAGTATA ACATGGACCT CTATGTTCTG
CGCTTAGCAG AGGACGGTCC TGCGGAGAGG TGTGGAAAGA TGAGGATTGG TGATGAAATT
TTAGAGATCA ATGGTGAGAC CACCAAAAAC ATGAAGCATT CTCGAGCTAT AGAACTGATT
AAGAATGGTG GCCGCAGAGT TCGTCTGTTT CTGAAGCGGG GAGACGGCTC AGTACCAGAA
TATGACCCCA GCAGCGACCG CCACGGCCCC GCCACCGGTC CACAAGGTGT TCCGGAAGTG
AGGGCCGGGC CCGACCGCCG GCAGCATCCG TCATTGGAGT CCAGTTACCC ACCCGATCTT
CACAAATCAT CACCACATGG GGAAAAGCGG GCACACGCAA GGGATCCGAA AGGCAGCAGA
GAGTATAGCA GACAACCCAA TGAACACCAC ACCTGGAATG GGACTTCGAG GAAACCCGAC
AGCGGGGCAT GCCGACCCAA GGACCGGGCG CCGGAGGGAC GGAGGGACGC GCAGGCCGAG
CGCGCGGCAG CCGCCAACGG CCCCAAGAGG CGGTCCCCAG AGAAGCGGAG GGAGGGCACC
CGCAGCGCCG ACAACACTTT GGAGAGGAGG GAGAAGCACG AGAAGAGACG AGACGTCTCT
CCGGAGCGGA GGCGAGAGCG GTCACCCACC CGCAGGAGAG ACGGCTCCCC CAGCCGGCGG
AGACGGTCTC TGGAGAGACT CCTGGAGCAG AGGAGGTCCC CCGAGCGCAG GAGAGGGGGC
TCGCCCGAGC GCAGGGCCAA GTCCACCGAC CGGAGGCGCG CACGCTCCCC CGAGCGCAGG
AGAGAGCGGT CCCTGGACAA AAGGAACAGA GAGGACAGAG CCAGCCACCG AGAAAGGGAA
GAGGCGAATC TGAAACAGGA TGCCGGCAGA AGTTCCAGAC ATCCCCCGGA GCAGAGAAGG
CGACCTTACA AAGAATGTAG CACCGACCTC AGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713471.1
CDS529..5007
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1407(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1435..1524(+)
Misc Feature(6)1477..1512(+)
Misc Feature(7)1615..1707(+)
Misc Feature(8)1657..1692(+)
Misc Feature(9)1957..2160(+)
Misc Feature(10)1972..2151(+)
Misc Feature(11)2452..2691(+)
Misc Feature(12)2485..2652(+)
Misc Feature(13)3070..3297(+)
Misc Feature(14)3079..3258(+)
Misc Feature(15)3517..3804(+)
Misc Feature(16)3553..3771(+)
Misc Feature(17)3982..4215(+)
Misc Feature(18)4012..4191(+)
Translation

Target ORF information:

RefSeq Version XM_006713408
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006713408

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGAAGGG GTACACACCG AGGAGCTGGA CAGTGAACTA
GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
CACATCAACA GGAAGACACA ATATGAGAAC CCGGTTCTAG AAGCCAAACG GAAGAAGCAG
CTTGAGCAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
CAGCAGACAG AAGAATGGAC AGAAGATCAC TCAGCCCTTG TGCCTCCTGT TATTCCAAAC
CACCCTCCAA GCAATCCAGA GCCAGCCAGA GAAGTTCCAC TTCAGGGCAA ACCCTTTTTT
ACACGAAACC CTTCTGAGTT GAAAGGCAAG TTCATTCACA CAAAGCTGCG GAAAAGCAGT
CGTGGCTTTG GCTTCACGGT GGTTGGAGGG GATGAACCTG ATGAGTTTCT CCAGATCAAG
AGCTTGGTCC TAGATGGTCC TGCTGCATTG GATGGCAAGA TGGAAACAGG GGATGTGATT
GTAAGTGTGA ATGACACCTG TGTTTTGGGA CACACACATG CTCAAGTTGT GAAGATCTTC
CAGTCCATCC CCATTGGTGC CAGCGTGGAC CTTGAACTCT GCCGAGGTTA TCCATTGCCT
TTTGATCCAG ATGACCCCAA TACAAGTTTA GTGACCTCGG TAGCCATTTT GGATAAAGAA
CCAATTATTG TGAATGGGCA AGAGACCTAT GATTCACCAG CTAGCCACAG TAGTAAAACA
GGCAAAGTCA ATGGCATGAA GGATGCCAGG CCAAGCAGCC CAGCGGACGT GGCTTCAAAT
AGTTCTCATG GTTATCCTAA TGACACTGTT TCTTTGGCTT CCTCCATAGC CACTCAGCCA
GAACTCATAA CTGTTCATAT TGTCAAAGGG CCAATGGGCT TTGGTTTTAC TATCGCAGAC
AGTCCTGGTG GGGGTGGCCA AAGAGTGAAA CAGATTGTTG ACAGCCCAAG GTGCCGAGGC
CTGAAAGAAG GGGATCTCAT AGTGGAAGTT AATAAGAAGA ACGTGCAGGC CCTAACTCAC
AACCAAGTGG TGGATATGCT GGTTGAGTGT CCTAAGGGAA GTGAGGTCAC ATTGTTGGTG
CAACGAGGAG GGCTGCCAGT TCCCAAGAAG AGCCCAAAGT CGCAACCACT GGAAAGGAAA
GACAGCCAGA ATAGTTCTCA GCACAGTGTT TCCAGCCACC GAAGCCTGCA CACAGCATCC
CCAAGCCACA GCACACAGGT GCTCCCCGAG TTCCCACCTG CAGAGGCCCA AGCTCCAGAT
CAAACTGACA GCTCTGGCCA GAAAAAACCA GATCCTTTTA AAATCTGGGC CCAGTCCAGG
AGCATGTATG AAAACCGACC TATGTCACCT TCACCTGCGT CGGGATTGAG CAAGGGTGAG
AGAGAGAGAG AAATCAATTC CACGAATTTT GGAGAATGTC CGATTCCAGA TTACCAGGAA
CAGGACATCT TCCTCTGGAG AAAAGAGACT GGATTTGGAT TTAGGATTCT GGGTGGAAAT
GAACCAGGGG AACCTATTTA TATTGGTCAC ATCGTACCAC TGGGTGCTGC TGATACTGAC
GGCCGCCTGA GGTCTGGAGA TGAATTAATC TGTGTGGATG GGACGCCAGT AATTGGAAAA
TCACACCAGC TTGTGGTCCA GCTTATGCAA CAAGCTGCCA AGCAAGGCCA CGTCAATCTC
ACGGTGCGGC GTAAAGTGGT TTTTGCGGTG CCCAAAACCG AGAACGAGGT GCCCTCGCCA
GCCTCCTCTC ATCACAGTAG CAACCAGCCG GCCTCGCTGA CAGAAGAGAA GCGCACTCCG
CAGGGCAGCC AGAACTCGCT GAACACGGTG AGCTCGGGCA GCGGCAGCAC CAGCGGCATC
GGCAGTGGCG GCGGCGGGGG CAGCGGCGTG GTCAGCACCG TGGTGCAGCC CTACGACGTG
GAGATCCGGC GCGGGGAGAA CGAGGGCTTC GGCTTCGTCA TCGTGTCCTC GGTGAGCAGG
CCCGAAGCAG GCACGACCTT TGCAGGCAAT GCATGTGTGG CTATGCCTCA CAAAATAGGT
CGGATTATTG AGGGGAGCCC TGCTGACCGC TGTGGCAAGC TGAAAGTAGG AGACCGGATC
TTGGCAGTAA ATGGATGTTC CATCACCAAC AAATCCCATT CAGACATTGT GAACCTAATC
AAGGAAGCGG GAAACACAGT TACCCTCCGC ATCATTCCTG GGGATGAGTC CTCGAATGCC
ACCTTGCTGA CCAATGCAGA GAAGATTGCC ACCATCACCA CCACACACAC CCCTTCTCAG
CAAGGGACCC AGGAGACAAG GAATACCACC AAACCAAAGC AGGAATCTCA ATTTGAGTTC
AAAGCACCCC AAGCAACACA GGAGCAAGAT TTTTACACTG TGGAACTGGA AAGAGGAGCC
AAGGGATTTG GCTTTAGCCT TCGAGGAGGC CGAGAGTATA ACATGGACCT CTATGTTCTG
CGCTTAGCAG AGGACGGTCC TGCGGAGAGG TGTGGAAAGA TGAGGATTGG TGATGAAATT
TTAGAGATCA ATGGTGAGAC CACCAAAAAC ATGAAGCATT CTCGAGCTAT AGAACTGATT
AAGAATGGTG GCCGCAGAGT TCGTCTGTTT CTGAAGCGGG GAGACGGCTC AGTACCAGAA
TATGACCCCA GCAGCGACCG CCACGGCCCC GCCACCGGTC CACAAGGTGT TCCGGAAGTG
AGGGCCGGGC CCGACCGCCG GCAGCATCCG TCATTGGAGT CCAGTTACCC ACCCGATCTT
CACAAATCAT CACCACATGG GGAAAAGCGG GCACACGCAA GGGATCCGAA AGGCAGCAGA
GAGTATAGCA GACAACCCAA TGAACACCAC ACCTGGAATG GGACTTCGAG GAAACCCGAC
AGCGGGGCAT GCCGACCCAA GGACCGGGCG CCGGAGGGAC GGAGGGACGC GCAGGCCGAG
CGCGCGGCAG CCGCCAACGG CCCCAAGAGG CGGTCCCCAG AGAAGCGGAG GGAGGGCACC
CGCAGCGCCG ACAACACTTT GGAGAGGAGG GAGAAGCACG AGAAGAGACG AGACGTCTCT
CCGGAGCGGA GGCGAGAGCG GTCACCCACC CGCAGGAGAG ACGGCTCCCC CAGCCGGCGG
AGACGGTCTC TGGAGAGACT CCTGGAGCAG AGGAGGTCCC CCGAGCGCAG GAGAGGGGGC
TCGCCCGAGC GCAGGGCCAA GTCCACCGAC CGGAGGCGCG CACGCTCCCC CGAGCGCAGG
AGAGAGCGGT CCCTGGACAA AAGGAACAGA GAGGACAGAG CCAGCCACCG AGAAAGGGAA
GAGGCGAATC TGAAACAGGA TGCCGGCAGA AGTTCCAGAC ATCCCCCGGA GCAGAGAAGG
CGACCTTACA AAGAATGTAG CACCGACCTC AGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37585
Clone ID Related Accession (Same CDS sequence) XM_006713409
Accession Version XM_006713409.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4479bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGGG GTACACACCG AGGAGCTGGA CAGTGAACTA
GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
CACATCAACA GGAAGACACA ATATGAGAAC CCGGTTCTAG AAGCCAAACG GAAGAAGCAG
CTTGAGCAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
CAGCAGACAG AAGAATGGAC AGAAGATCAC TCAGCCCTTG TGCCTCCTGT TATTCCAAAC
CACCCTCCAA GCAATCCAGA GCCAGCCAGA GAAGTTCCAC TTCAGGGCAA ACCCTTTTTT
ACACGAAACC CTTCTGAGTT GAAAGGCAAG TTCATTCACA CAAAGCTGCG GAAAAGCAGT
CGTGGCTTTG GCTTCACGGT GGTTGGAGGG GATGAACCTG ATGAGTTTCT CCAGATCAAG
AGCTTGGTCC TAGATGGTCC TGCTGCATTG GATGGCAAGA TGGAAACAGG GGATGTGATT
GTAAGTGTGA ATGACACCTG TGTTTTGGGA CACACACATG CTCAAGTTGT GAAGATCTTC
CAGTCCATCC CCATTGGTGC CAGCGTGGAC CTTGAACTCT GCCGAGGTTA TCCATTGCCT
TTTGATCCAG ATGACCCCAA TACAAGTTTA GTGACCTCGG TAGCCATTTT GGATAAAGAA
CCAATTATTG TGAATGGGCA AGAGACCTAT GATTCACCAG CTAGCCACAG TAGTAAAACA
GGCAAAGTCA ATGGCATGAA GGATGCCAGG CCAAGCAGCC CAGCGGACGT GGCTTCAAAT
AGTTCTCATG GTTATCCTAA TGACACTGTT TCTTTGGCTT CCTCCATAGC CACTCAGCCA
GAACTCATAA CTGTTCATAT TGTCAAAGGG CCAATGGGCT TTGGTTTTAC TATCGCAGAC
AGTCCTGGTG GGGGTGGCCA AAGAGTGAAA CAGATTGTTG ACAGCCCAAG GTGCCGAGGC
CTGAAAGAAG GGGATCTCAT AGTGGAAGTT AATAAGAAGA ACGTGCAGGC CCTAACTCAC
AACCAAGTGG TGGATATGCT GGTTGAGTGT CCTAAGGGAA GTGAGGTCAC ATTGTTGGTG
CAACGAGGAG GGCTGCCAGT TCCCAAGAAG AGCCCAAAGT CGCAACCACT GGAAAGGAAA
GACAGCCAGA ATAGTTCTCA GCACAGTGTT TCCAGCCACC GAAGCCTGCA CACAGCATCC
CCAAGCCACA GCACACAGGT GCTCCCCGAG TTCCCACCTG CAGAGGCCCA AGCTCCAGAT
CAAACTGACA GCTCTGGCCA GAAAAAACCA GATCCTTTTA AAATCTGGGC CCAGTCCAGG
AGCATGTATG AAAACCGACC TATGTCACCT TCACCTGCGT CGGGATTGAG CAAGGGTGAG
AGAGAGAGAG AAATCAATTC CACGAATTTT GGAGAATGTC CGATTCCAGA TTACCAGGAA
CAGGACATCT TCCTCTGGAG AAAAGAGACT GGATTTGGAT TTAGGATTCT GGGTGGAAAT
GAACCAGGGG AACCTATTTA TATTGGTCAC ATCGTACCAC TGGGTGCTGC TGATACTGAC
GGCCGCCTGA GGTCTGGAGA TGAATTAATC TGTGTGGATG GGACGCCAGT AATTGGAAAA
TCACACCAGC TTGTGGTCCA GCTTATGCAA CAAGCTGCCA AGCAAGGCCA CGTCAATCTC
ACGGTGCGGC GTAAAGTGGT TTTTGCGGTG CCCAAAACCG AGAACGAGGT GCCCTCGCCA
GCCTCCTCTC ATCACAGTAG CAACCAGCCG GCCTCGCTGA CAGAAGAGAA GCGCACTCCG
CAGGGCAGCC AGAACTCGCT GAACACGGTG AGCTCGGGCA GCGGCAGCAC CAGCGGCATC
GGCAGTGGCG GCGGCGGGGG CAGCGGCGTG GTCAGCACCG TGGTGCAGCC CTACGACGTG
GAGATCCGGC GCGGGGAGAA CGAGGGCTTC GGCTTCGTCA TCGTGTCCTC GGTGAGCAGG
CCCGAAGCAG GCACGACCTT TGCAGGCAAT GCATGTGTGG CTATGCCTCA CAAAATAGGT
CGGATTATTG AGGGGAGCCC TGCTGACCGC TGTGGCAAGC TGAAAGTAGG AGACCGGATC
TTGGCAGTAA ATGGATGTTC CATCACCAAC AAATCCCATT CAGACATTGT GAACCTAATC
AAGGAAGCGG GAAACACAGT TACCCTCCGC ATCATTCCTG GGGATGAGTC CTCGAATGCC
ACCTTGCTGA CCAATGCAGA GAAGATTGCC ACCATCACCA CCACACACAC CCCTTCTCAG
CAAGGGACCC AGGAGACAAG GAATACCACC AAACCAAAGC AGGAATCTCA ATTTGAGTTC
AAAGCACCCC AAGCAACACA GGAGCAAGAT TTTTACACTG TGGAACTGGA AAGAGGAGCC
AAGGGATTTG GCTTTAGCCT TCGAGGAGGC CGAGAGTATA ACATGGACCT CTATGTTCTG
CGCTTAGCAG AGGACGGTCC TGCGGAGAGG TGTGGAAAGA TGAGGATTGG TGATGAAATT
TTAGAGATCA ATGGTGAGAC CACCAAAAAC ATGAAGCATT CTCGAGCTAT AGAACTGATT
AAGAATGGTG GCCGCAGAGT TCGTCTGTTT CTGAAGCGGG GAGACGGCTC AGTACCAGAA
TATGACCCCA GCAGCGACCG CCACGGCCCC GCCACCGGTC CACAAGGTGT TCCGGAAGTG
AGGGCCGGGC CCGACCGCCG GCAGCATCCG TCATTGGAGT CCAGTTACCC ACCCGATCTT
CACAAATCAT CACCACATGG GGAAAAGCGG GCACACGCAA GGGATCCGAA AGGCAGCAGA
GAGTATAGCA GACAACCCAA TGAACACCAC ACCTGGAATG GGACTTCGAG GAAACCCGAC
AGCGGGGCAT GCCGACCCAA GGACCGGGCG CCGGAGGGAC GGAGGGACGC GCAGGCCGAG
CGCGCGGCAG CCGCCAACGG CCCCAAGAGG CGGTCCCCAG AGAAGCGGAG GGAGGGCACC
CGCAGCGCCG ACAACACTTT GGAGAGGAGG GAGAAGCACG AGAAGAGACG AGACGTCTCT
CCGGAGCGGA GGCGAGAGCG GTCACCCACC CGCAGGAGAG ACGGCTCCCC CAGCCGGCGG
AGACGGTCTC TGGAGAGACT CCTGGAGCAG AGGAGGTCCC CCGAGCGCAG GAGAGGGGGC
TCGCCCGAGC GCAGGGCCAA GTCCACCGAC CGGAGGCGCG CACGCTCCCC CGAGCGCAGG
AGAGAGCGGT CCCTGGACAA AAGGAACAGA GAGGACAGAG CCAGCCACCG AGAAAGGGAA
GAGGCGAATC TGAAACAGGA TGCCGGCAGA AGTTCCAGAC ATCCCCCGGA GCAGAGAAGG
CGACCTTACA AAGAATGTAG CACCGACCTC AGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713472.1
CDS529..5007
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1410(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1438..1527(+)
Misc Feature(6)1480..1515(+)
Misc Feature(7)1615..1707(+)
Misc Feature(8)1657..1692(+)
Misc Feature(9)1957..2160(+)
Misc Feature(10)1972..2151(+)
Misc Feature(11)2452..2691(+)
Misc Feature(12)2485..2652(+)
Misc Feature(13)3070..3297(+)
Misc Feature(14)3079..3258(+)
Misc Feature(15)3517..3804(+)
Misc Feature(16)3553..3771(+)
Misc Feature(17)3982..4215(+)
Misc Feature(18)4012..4191(+)
Translation

Target ORF information:

RefSeq Version XM_006713409
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006713409

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGGG GTACACACCG AGGAGCTGGA CAGTGAACTA
GAACTGCCTG CTGGTTGGGA AAAGATTGAA GACCCTGTCT ATGGTATCTA CTATGTAGAC
CACATCAACA GGAAGACACA ATATGAGAAC CCGGTTCTAG AAGCCAAACG GAAGAAGCAG
CTTGAGCAGC AGCAGCAGCA GCAACAGCAG CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
CAGCAGACAG AAGAATGGAC AGAAGATCAC TCAGCCCTTG TGCCTCCTGT TATTCCAAAC
CACCCTCCAA GCAATCCAGA GCCAGCCAGA GAAGTTCCAC TTCAGGGCAA ACCCTTTTTT
ACACGAAACC CTTCTGAGTT GAAAGGCAAG TTCATTCACA CAAAGCTGCG GAAAAGCAGT
CGTGGCTTTG GCTTCACGGT GGTTGGAGGG GATGAACCTG ATGAGTTTCT CCAGATCAAG
AGCTTGGTCC TAGATGGTCC TGCTGCATTG GATGGCAAGA TGGAAACAGG GGATGTGATT
GTAAGTGTGA ATGACACCTG TGTTTTGGGA CACACACATG CTCAAGTTGT GAAGATCTTC
CAGTCCATCC CCATTGGTGC CAGCGTGGAC CTTGAACTCT GCCGAGGTTA TCCATTGCCT
TTTGATCCAG ATGACCCCAA TACAAGTTTA GTGACCTCGG TAGCCATTTT GGATAAAGAA
CCAATTATTG TGAATGGGCA AGAGACCTAT GATTCACCAG CTAGCCACAG TAGTAAAACA
GGCAAAGTCA ATGGCATGAA GGATGCCAGG CCAAGCAGCC CAGCGGACGT GGCTTCAAAT
AGTTCTCATG GTTATCCTAA TGACACTGTT TCTTTGGCTT CCTCCATAGC CACTCAGCCA
GAACTCATAA CTGTTCATAT TGTCAAAGGG CCAATGGGCT TTGGTTTTAC TATCGCAGAC
AGTCCTGGTG GGGGTGGCCA AAGAGTGAAA CAGATTGTTG ACAGCCCAAG GTGCCGAGGC
CTGAAAGAAG GGGATCTCAT AGTGGAAGTT AATAAGAAGA ACGTGCAGGC CCTAACTCAC
AACCAAGTGG TGGATATGCT GGTTGAGTGT CCTAAGGGAA GTGAGGTCAC ATTGTTGGTG
CAACGAGGAG GGCTGCCAGT TCCCAAGAAG AGCCCAAAGT CGCAACCACT GGAAAGGAAA
GACAGCCAGA ATAGTTCTCA GCACAGTGTT TCCAGCCACC GAAGCCTGCA CACAGCATCC
CCAAGCCACA GCACACAGGT GCTCCCCGAG TTCCCACCTG CAGAGGCCCA AGCTCCAGAT
CAAACTGACA GCTCTGGCCA GAAAAAACCA GATCCTTTTA AAATCTGGGC CCAGTCCAGG
AGCATGTATG AAAACCGACC TATGTCACCT TCACCTGCGT CGGGATTGAG CAAGGGTGAG
AGAGAGAGAG AAATCAATTC CACGAATTTT GGAGAATGTC CGATTCCAGA TTACCAGGAA
CAGGACATCT TCCTCTGGAG AAAAGAGACT GGATTTGGAT TTAGGATTCT GGGTGGAAAT
GAACCAGGGG AACCTATTTA TATTGGTCAC ATCGTACCAC TGGGTGCTGC TGATACTGAC
GGCCGCCTGA GGTCTGGAGA TGAATTAATC TGTGTGGATG GGACGCCAGT AATTGGAAAA
TCACACCAGC TTGTGGTCCA GCTTATGCAA CAAGCTGCCA AGCAAGGCCA CGTCAATCTC
ACGGTGCGGC GTAAAGTGGT TTTTGCGGTG CCCAAAACCG AGAACGAGGT GCCCTCGCCA
GCCTCCTCTC ATCACAGTAG CAACCAGCCG GCCTCGCTGA CAGAAGAGAA GCGCACTCCG
CAGGGCAGCC AGAACTCGCT GAACACGGTG AGCTCGGGCA GCGGCAGCAC CAGCGGCATC
GGCAGTGGCG GCGGCGGGGG CAGCGGCGTG GTCAGCACCG TGGTGCAGCC CTACGACGTG
GAGATCCGGC GCGGGGAGAA CGAGGGCTTC GGCTTCGTCA TCGTGTCCTC GGTGAGCAGG
CCCGAAGCAG GCACGACCTT TGCAGGCAAT GCATGTGTGG CTATGCCTCA CAAAATAGGT
CGGATTATTG AGGGGAGCCC TGCTGACCGC TGTGGCAAGC TGAAAGTAGG AGACCGGATC
TTGGCAGTAA ATGGATGTTC CATCACCAAC AAATCCCATT CAGACATTGT GAACCTAATC
AAGGAAGCGG GAAACACAGT TACCCTCCGC ATCATTCCTG GGGATGAGTC CTCGAATGCC
ACCTTGCTGA CCAATGCAGA GAAGATTGCC ACCATCACCA CCACACACAC CCCTTCTCAG
CAAGGGACCC AGGAGACAAG GAATACCACC AAACCAAAGC AGGAATCTCA ATTTGAGTTC
AAAGCACCCC AAGCAACACA GGAGCAAGAT TTTTACACTG TGGAACTGGA AAGAGGAGCC
AAGGGATTTG GCTTTAGCCT TCGAGGAGGC CGAGAGTATA ACATGGACCT CTATGTTCTG
CGCTTAGCAG AGGACGGTCC TGCGGAGAGG TGTGGAAAGA TGAGGATTGG TGATGAAATT
TTAGAGATCA ATGGTGAGAC CACCAAAAAC ATGAAGCATT CTCGAGCTAT AGAACTGATT
AAGAATGGTG GCCGCAGAGT TCGTCTGTTT CTGAAGCGGG GAGACGGCTC AGTACCAGAA
TATGACCCCA GCAGCGACCG CCACGGCCCC GCCACCGGTC CACAAGGTGT TCCGGAAGTG
AGGGCCGGGC CCGACCGCCG GCAGCATCCG TCATTGGAGT CCAGTTACCC ACCCGATCTT
CACAAATCAT CACCACATGG GGAAAAGCGG GCACACGCAA GGGATCCGAA AGGCAGCAGA
GAGTATAGCA GACAACCCAA TGAACACCAC ACCTGGAATG GGACTTCGAG GAAACCCGAC
AGCGGGGCAT GCCGACCCAA GGACCGGGCG CCGGAGGGAC GGAGGGACGC GCAGGCCGAG
CGCGCGGCAG CCGCCAACGG CCCCAAGAGG CGGTCCCCAG AGAAGCGGAG GGAGGGCACC
CGCAGCGCCG ACAACACTTT GGAGAGGAGG GAGAAGCACG AGAAGAGACG AGACGTCTCT
CCGGAGCGGA GGCGAGAGCG GTCACCCACC CGCAGGAGAG ACGGCTCCCC CAGCCGGCGG
AGACGGTCTC TGGAGAGACT CCTGGAGCAG AGGAGGTCCC CCGAGCGCAG GAGAGGGGGC
TCGCCCGAGC GCAGGGCCAA GTCCACCGAC CGGAGGCGCG CACGCTCCCC CGAGCGCAGG
AGAGAGCGGT CCCTGGACAA AAGGAACAGA GAGGACAGAG CCAGCCACCG AGAAAGGGAA
GAGGCGAATC TGAAACAGGA TGCCGGCAGA AGTTCCAGAC ATCCCCCGGA GCAGAGAAGG
CGACCTTACA AAGAATGTAG CACCGACCTC AGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37586
Clone ID Related Accession (Same CDS sequence) XM_005265563
Accession Version XM_005265563.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4479bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACCTATGTCA CCTTCACCTG CGTCGGGATT GAGCAAGGGT
GAGAGAGAGA GAGAAATCAA TTCCACGAAT TTTGGAGAAT GTCCGATTCC AGATTACCAG
GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACTGGATTTG GATTTAGGAT TCTGGGTGGA
AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACT
GACGGCCGCC TGAGGTCTGG AGATGAATTA ATCTGTGTGG ATGGGACGCC AGTAATTGGA
AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCTG CCAAGCAAGG CCACGTCAAT
CTCACGGTGC GGCGTAAAGT GGTTTTTGCG GTGCCCAAAA CCGAGAACGA GGTGCCCTCG
CCAGCCTCCT CTCATCACAG TAGCAACCAG CCGGCCTCGC TGACAGAAGA GAAGCGCACT
CCGCAGGGCA GCCAGAACTC GCTGAACACG GTGAGCTCGG GCAGCGGCAG CACCAGCGGC
ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC GTGGTCAGCA CCGTGGTGCA GCCCTACGAC
GTGGAGATCC GGCGCGGGGA GAACGAGGGC TTCGGCTTCG TCATCGTGTC CTCGGTGAGC
AGGCCCGAAG CAGGCACGAC CTTTGGCAAT GCATGTGTGG CTATGCCTCA CAAAATAGGT
CGGATTATTG AGGGGAGCCC TGCTGACCGC TGTGGCAAGC TGAAAGTAGG AGACCGGATC
TTGGCAGTAA ATGGATGTTC CATCACCAAC AAATCCCATT CAGACATTGT GAACCTAATC
AAGGAAGCGG GAAACACAGT TACCCTCCGC ATCATTCCTG GGGATGAGTC CTCGAATGCC
ACCTTGCTGA CCAATGCAGA GAAGATTGCC ACCATCACCA CCACACACAC CCCTTCTCAG
CAAGGGACCC AGGAGACAAG GAATACCACC AAACCAAAGC AGGAATCTCA ATTTGAGTTC
AAAGCACCCC AAGCAACACA GGAGCAAGAT TTTTACACTG TGGAACTGGA AAGAGGAGCC
AAGGGATTTG GCTTTAGCCT TCGAGGAGGC CGAGAGTATA ACATGGACCT CTATGTTCTG
CGCTTAGCAG AGGACGGTCC TGCGGAGAGG TGTGGAAAGA TGAGGATTGG TGATGAAATT
TTAGAGATCA ATGGTGAGAC CACCAAAAAC ATGAAGCATT CTCGAGCTAT AGAACTGATT
AAGAATGGTG GCCGCAGAGT TCGTCTGTTT CTGAAGCGGG GAGACGGCTC AGTACCAGAA
TATGACCCCA GCAGCGACCG CCACGGCCCC GCCACCGGTC CACAAGGTGT TCCGGAAGTG
AGGGCCGGGC CCGACCGCCG GCAGCATCCG TCATTGGAGT CCAGTTACCC ACCCGATCTT
CACAAATCAT CACCACATGG GGAAAAGCGG GCACACGCAA GGGATCCGAA AGGCAGCAGA
GAGTATAGCA GACAACCCAA TGAACACCAC ACCTGGAATG GGACTTCGAG GAAACCCGAC
AGCGGGGCAT GCCGACCCAA GGACCGGGCG CCGGAGGGAC GGAGGGACGC GCAGGCCGAG
CGCGCGGCAG CCGCCAACGG CCCCAAGAGG CGGTCCCCAG AGAAGCGGAG GGAGGGCACC
CGCAGCGCCG ACAACACTTT GGAGAGGAGG GAGAAGCACG AGAAGAGACG AGACGTCTCT
CCGGAGCGGA GGCGAGAGCG GTCACCCACC CGCAGGAGAG ACGGCTCCCC CAGCCGGCGG
AGACGGTCTC TGGAGAGACT CCTGGAGCAG AGGAGGTCCC CCGAGCGCAG GAGAGGGGGC
TCGCCCGAGC GCAGGGCCAA GTCCACCGAC CGGAGGCGCG CACGCTCCCC CGAGCGCAGG
AGAGAGCGGT CCCTGGACAA AAGGAACAGA GAGGACAGAG CCAGCCACCG AGAAAGGGAA
GAGGCGAATC TGAAACAGGA TGCCGGCAGA AGTTCCAGAC ATCCCCCGGA GCAGAGAAGG
CGACCTTACA AAGAATGTAG CACCGACCTC AGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265620.1
CDS529..5007
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1410(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1438..1527(+)
Misc Feature(6)1480..1515(+)
Misc Feature(7)1618..1710(+)
Misc Feature(8)1660..1695(+)
Misc Feature(9)1960..2163(+)
Misc Feature(10)1975..2154(+)
Misc Feature(11)2455..2694(+)
Misc Feature(12)2488..2655(+)
Misc Feature(13)3073..3300(+)
Misc Feature(14)3082..3261(+)
Misc Feature(15)3520..3804(+)
Misc Feature(16)3556..3771(+)
Misc Feature(17)3982..4215(+)
Misc Feature(18)4012..4191(+)
Translation

Target ORF information:

RefSeq Version XM_005265563
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265563

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACCTATGTCA CCTTCACCTG CGTCGGGATT GAGCAAGGGT
GAGAGAGAGA GAGAAATCAA TTCCACGAAT TTTGGAGAAT GTCCGATTCC AGATTACCAG
GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACTGGATTTG GATTTAGGAT TCTGGGTGGA
AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACT
GACGGCCGCC TGAGGTCTGG AGATGAATTA ATCTGTGTGG ATGGGACGCC AGTAATTGGA
AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCTG CCAAGCAAGG CCACGTCAAT
CTCACGGTGC GGCGTAAAGT GGTTTTTGCG GTGCCCAAAA CCGAGAACGA GGTGCCCTCG
CCAGCCTCCT CTCATCACAG TAGCAACCAG CCGGCCTCGC TGACAGAAGA GAAGCGCACT
CCGCAGGGCA GCCAGAACTC GCTGAACACG GTGAGCTCGG GCAGCGGCAG CACCAGCGGC
ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC GTGGTCAGCA CCGTGGTGCA GCCCTACGAC
GTGGAGATCC GGCGCGGGGA GAACGAGGGC TTCGGCTTCG TCATCGTGTC CTCGGTGAGC
AGGCCCGAAG CAGGCACGAC CTTTGGCAAT GCATGTGTGG CTATGCCTCA CAAAATAGGT
CGGATTATTG AGGGGAGCCC TGCTGACCGC TGTGGCAAGC TGAAAGTAGG AGACCGGATC
TTGGCAGTAA ATGGATGTTC CATCACCAAC AAATCCCATT CAGACATTGT GAACCTAATC
AAGGAAGCGG GAAACACAGT TACCCTCCGC ATCATTCCTG GGGATGAGTC CTCGAATGCC
ACCTTGCTGA CCAATGCAGA GAAGATTGCC ACCATCACCA CCACACACAC CCCTTCTCAG
CAAGGGACCC AGGAGACAAG GAATACCACC AAACCAAAGC AGGAATCTCA ATTTGAGTTC
AAAGCACCCC AAGCAACACA GGAGCAAGAT TTTTACACTG TGGAACTGGA AAGAGGAGCC
AAGGGATTTG GCTTTAGCCT TCGAGGAGGC CGAGAGTATA ACATGGACCT CTATGTTCTG
CGCTTAGCAG AGGACGGTCC TGCGGAGAGG TGTGGAAAGA TGAGGATTGG TGATGAAATT
TTAGAGATCA ATGGTGAGAC CACCAAAAAC ATGAAGCATT CTCGAGCTAT AGAACTGATT
AAGAATGGTG GCCGCAGAGT TCGTCTGTTT CTGAAGCGGG GAGACGGCTC AGTACCAGAA
TATGACCCCA GCAGCGACCG CCACGGCCCC GCCACCGGTC CACAAGGTGT TCCGGAAGTG
AGGGCCGGGC CCGACCGCCG GCAGCATCCG TCATTGGAGT CCAGTTACCC ACCCGATCTT
CACAAATCAT CACCACATGG GGAAAAGCGG GCACACGCAA GGGATCCGAA AGGCAGCAGA
GAGTATAGCA GACAACCCAA TGAACACCAC ACCTGGAATG GGACTTCGAG GAAACCCGAC
AGCGGGGCAT GCCGACCCAA GGACCGGGCG CCGGAGGGAC GGAGGGACGC GCAGGCCGAG
CGCGCGGCAG CCGCCAACGG CCCCAAGAGG CGGTCCCCAG AGAAGCGGAG GGAGGGCACC
CGCAGCGCCG ACAACACTTT GGAGAGGAGG GAGAAGCACG AGAAGAGACG AGACGTCTCT
CCGGAGCGGA GGCGAGAGCG GTCACCCACC CGCAGGAGAG ACGGCTCCCC CAGCCGGCGG
AGACGGTCTC TGGAGAGACT CCTGGAGCAG AGGAGGTCCC CCGAGCGCAG GAGAGGGGGC
TCGCCCGAGC GCAGGGCCAA GTCCACCGAC CGGAGGCGCG CACGCTCCCC CGAGCGCAGG
AGAGAGCGGT CCCTGGACAA AAGGAACAGA GAGGACAGAG CCAGCCACCG AGAAAGGGAA
GAGGCGAATC TGAAACAGGA TGCCGGCAGA AGTTCCAGAC ATCCCCCGGA GCAGAGAAGG
CGACCTTACA AAGAATGTAG CACCGACCTC AGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37587
Clone ID Related Accession (Same CDS sequence) XM_005265564
Accession Version XM_005265564.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACCTAT GTCACCTTCA CCTGCGTCGG GATTGAGCAA GGGTGAGAGA
GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCCGA TTCCAGATTA CCAGGAACAG
GACATCTTCC TCTGGAGAAA AGAGACTGGA TTTGGATTTA GGATTCTGGG TGGAAATGAA
CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACTGACGGC
CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA CGCCAGTAAT TGGAAAATCA
CACCAGCTTG TGGTCCAGCT TATGCAACAA GCTGCCAAGC AAGGCCACGT CAATCTCACG
GTGCGGCGTA AAGTGGTTTT TGCGGTGCCC AAAACCGAGA ACGAGGTGCC CTCGCCAGCC
TCCTCTCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACAG AAGAGAAGCG CACTCCGCAG
GGCAGCCAGA ACTCGCTGAA CACGGTGAGC TCGGGCAGCG GCAGCACCAG CGGCATCGGC
AGTGGCGGCG GCGGGGGCAG CGGCGTGGTC AGCACCGTGG TGCAGCCCTA CGACGTGGAG
ATCCGGCGCG GGGAGAACGA GGGCTTCGGC TTCGTCATCG TGTCCTCGGT GAGCAGGCCC
GAAGCAGGCA CGACCTTTGC AGGCAATGCA TGTGTGGCTA TGCCTCACAA AATAGGTCGG
ATTATTGAGG GGAGCCCTGC TGACCGCTGT GGCAAGCTGA AAGTAGGAGA CCGGATCTTG
GCAGTAAATG GATGTTCCAT CACCAACAAA TCCCATTCAG ACATTGTGAA CCTAATCAAG
GAAGCGGGAA ACACAGTTAC CCTCCGCATC ATTCCTGGGG ATGAGTCCTC GAATGCCACC
TTGCTGACCA ATGCAGAGAA GATTGCCACC ATCACCACCA CACACACCCC TTCTCAGCAA
GGGACCCAGG AGACAAGGAA TACCACCAAA CCAAAGCAGG AATCTCAATT TGAGTTCAAA
GCACCCCAAG CAACACAGGA GCAAGATTTT TACACTGTGG AACTGGAAAG AGGAGCCAAG
GGATTTGGCT TTAGCCTTCG AGGAGGCCGA GAGTATAACA TGGACCTCTA TGTTCTGCGC
TTAGCAGAGG ACGGTCCTGC GGAGAGGTGT GGAAAGATGA GGATTGGTGA TGAAATTTTA
GAGATCAATG GTGAGACCAC CAAAAACATG AAGCATTCTC GAGCTATAGA ACTGATTAAG
AATGGTGGCC GCAGAGTTCG TCTGTTTCTG AAGCGGGGAG ACGGCTCAGT ACCAGAATAT
GACCCCAGCA GCGACCGCCA CGGCCCCGCC ACCGGTCCAC AAGGTGTTCC GGAAGTGAGG
GCCGGGCCCG ACCGCCGGCA GCATCCGTCA TTGGAGTCCA GTTACCCACC CGATCTTCAC
AAATCATCAC CACATGGGGA AAAGCGGGCA CACGCAAGGG ATCCGAAAGG CAGCAGAGAG
TATAGCAGAC AACCCAATGA ACACCACACC TGGAATGGGA CTTCGAGGAA ACCCGACAGC
GGGGCATGCC GACCCAAGGA CCGGGCGCCG GAGGGACGGA GGGACGCGCA GGCCGAGCGC
GCGGCAGCCG CCAACGGCCC CAAGAGGCGG TCCCCAGAGA AGCGGAGGGA GGGCACCCGC
AGCGCCGACA ACACTTTGGA GAGGAGGGAG AAGCACGAGA AGAGACGAGA CGTCTCTCCG
GAGCGGAGGC GAGAGCGGTC ACCCACCCGC AGGAGAGACG GCTCCCCCAG CCGGCGGAGA
CGGTCTCTGG AGAGACTCCT GGAGCAGAGG AGGTCCCCCG AGCGCAGGAG AGGGGGCTCG
CCCGAGCGCA GGGCCAAGTC CACCGACCGG AGGCGCGCAC GCTCCCCCGA GCGCAGGAGA
GAGCGGTCCC TGGACAAAAG GAACAGAGAG GACAGAGCCA GCCACCGAGA AAGGGAAGAG
GCGAATCTGA AACAGGATGC CGGCAGAAGT TCCAGACATC CCCCGGAGCA GAGAAGGCGA
CCTTACAAAG AATGTAGCAC CGACCTCAGT ATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265621.1
CDS529..5004
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1407(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1435..1524(+)
Misc Feature(6)1477..1512(+)
Misc Feature(7)1612..1704(+)
Misc Feature(8)1654..1689(+)
Misc Feature(9)1954..2157(+)
Misc Feature(10)1969..2148(+)
Misc Feature(11)2449..2688(+)
Misc Feature(12)2482..2649(+)
Misc Feature(13)3067..3294(+)
Misc Feature(14)3076..3255(+)
Misc Feature(15)3514..3801(+)
Misc Feature(16)3550..3768(+)
Misc Feature(17)3979..4212(+)
Misc Feature(18)4009..4188(+)
Translation

Target ORF information:

RefSeq Version XM_005265564
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265564

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACCTAT GTCACCTTCA CCTGCGTCGG GATTGAGCAA GGGTGAGAGA
GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCCGA TTCCAGATTA CCAGGAACAG
GACATCTTCC TCTGGAGAAA AGAGACTGGA TTTGGATTTA GGATTCTGGG TGGAAATGAA
CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACTGACGGC
CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA CGCCAGTAAT TGGAAAATCA
CACCAGCTTG TGGTCCAGCT TATGCAACAA GCTGCCAAGC AAGGCCACGT CAATCTCACG
GTGCGGCGTA AAGTGGTTTT TGCGGTGCCC AAAACCGAGA ACGAGGTGCC CTCGCCAGCC
TCCTCTCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACAG AAGAGAAGCG CACTCCGCAG
GGCAGCCAGA ACTCGCTGAA CACGGTGAGC TCGGGCAGCG GCAGCACCAG CGGCATCGGC
AGTGGCGGCG GCGGGGGCAG CGGCGTGGTC AGCACCGTGG TGCAGCCCTA CGACGTGGAG
ATCCGGCGCG GGGAGAACGA GGGCTTCGGC TTCGTCATCG TGTCCTCGGT GAGCAGGCCC
GAAGCAGGCA CGACCTTTGC AGGCAATGCA TGTGTGGCTA TGCCTCACAA AATAGGTCGG
ATTATTGAGG GGAGCCCTGC TGACCGCTGT GGCAAGCTGA AAGTAGGAGA CCGGATCTTG
GCAGTAAATG GATGTTCCAT CACCAACAAA TCCCATTCAG ACATTGTGAA CCTAATCAAG
GAAGCGGGAA ACACAGTTAC CCTCCGCATC ATTCCTGGGG ATGAGTCCTC GAATGCCACC
TTGCTGACCA ATGCAGAGAA GATTGCCACC ATCACCACCA CACACACCCC TTCTCAGCAA
GGGACCCAGG AGACAAGGAA TACCACCAAA CCAAAGCAGG AATCTCAATT TGAGTTCAAA
GCACCCCAAG CAACACAGGA GCAAGATTTT TACACTGTGG AACTGGAAAG AGGAGCCAAG
GGATTTGGCT TTAGCCTTCG AGGAGGCCGA GAGTATAACA TGGACCTCTA TGTTCTGCGC
TTAGCAGAGG ACGGTCCTGC GGAGAGGTGT GGAAAGATGA GGATTGGTGA TGAAATTTTA
GAGATCAATG GTGAGACCAC CAAAAACATG AAGCATTCTC GAGCTATAGA ACTGATTAAG
AATGGTGGCC GCAGAGTTCG TCTGTTTCTG AAGCGGGGAG ACGGCTCAGT ACCAGAATAT
GACCCCAGCA GCGACCGCCA CGGCCCCGCC ACCGGTCCAC AAGGTGTTCC GGAAGTGAGG
GCCGGGCCCG ACCGCCGGCA GCATCCGTCA TTGGAGTCCA GTTACCCACC CGATCTTCAC
AAATCATCAC CACATGGGGA AAAGCGGGCA CACGCAAGGG ATCCGAAAGG CAGCAGAGAG
TATAGCAGAC AACCCAATGA ACACCACACC TGGAATGGGA CTTCGAGGAA ACCCGACAGC
GGGGCATGCC GACCCAAGGA CCGGGCGCCG GAGGGACGGA GGGACGCGCA GGCCGAGCGC
GCGGCAGCCG CCAACGGCCC CAAGAGGCGG TCCCCAGAGA AGCGGAGGGA GGGCACCCGC
AGCGCCGACA ACACTTTGGA GAGGAGGGAG AAGCACGAGA AGAGACGAGA CGTCTCTCCG
GAGCGGAGGC GAGAGCGGTC ACCCACCCGC AGGAGAGACG GCTCCCCCAG CCGGCGGAGA
CGGTCTCTGG AGAGACTCCT GGAGCAGAGG AGGTCCCCCG AGCGCAGGAG AGGGGGCTCG
CCCGAGCGCA GGGCCAAGTC CACCGACCGG AGGCGCGCAC GCTCCCCCGA GCGCAGGAGA
GAGCGGTCCC TGGACAAAAG GAACAGAGAG GACAGAGCCA GCCACCGAGA AAGGGAAGAG
GCGAATCTGA AACAGGATGC CGGCAGAAGT TCCAGACATC CCCCGGAGCA GAGAAGGCGA
CCTTACAAAG AATGTAGCAC CGACCTCAGT ATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37588
Clone ID Related Accession (Same CDS sequence) XM_005265565
Accession Version XM_005265565.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACCTATGTCA CCTTCACCTG CGTCGGGATT GAGCAAGGGT
GAGAGAGAGA GAGAAATCAA TTCCACGAAT TTTGGAGAAT GTCCGATTCC AGATTACCAG
GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACTGGATTTG GATTTAGGAT TCTGGGTGGA
AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACT
GACGGCCGCC TGAGGTCTGG AGATGAATTA ATCTGTGTGG ATGGGACGCC AGTAATTGGA
AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCTG CCAAGCAAGG CCACGTCAAT
CTCACGGTGC GGCGTAAAGT GGTTTTTGCG GTGCCCAAAA CCGAGAACGA GGTGCCCTCG
CCAGCCTCCT CTCATCACAG TAGCAACCAG CCGGCCTCGC TGACAGAAGA GAAGCGCACT
CCGCAGGGCA GCCAGAACTC GCTGAACACG GTGAGCTCGG GCAGCGGCAG CACCAGCGGC
ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC GTGGTCAGCA CCGTGGTGCA GCCCTACGAC
GTGGAGATCC GGCGCGGGGA GAACGAGGGC TTCGGCTTCG TCATCGTGTC CTCGGTGAGC
AGGCCCGAAG CAGGCACGAC CTTTGGTCGG ATTATTGAGG GGAGCCCTGC TGACCGCTGT
GGCAAGCTGA AAGTAGGAGA CCGGATCTTG GCAGTAAATG GATGTTCCAT CACCAACAAA
TCCCATTCAG ACATTGTGAA CCTAATCAAG GAAGCGGGAA ACACAGTTAC CCTCCGCATC
ATTCCTGGGG ATGAGTCCTC GAATGCCACC TTGCTGACCA ATGCAGAGAA GATTGCCACC
ATCACCACCA CACACACCCC TTCTCAGCAA GGGACCCAGG AGACAAGGAA TACCACCAAA
CCAAAGCAGG AATCTCAATT TGAGTTCAAA GCACCCCAAG CAACACAGGA GCAAGATTTT
TACACTGTGG AACTGGAAAG AGGAGCCAAG GGATTTGGCT TTAGCCTTCG AGGAGGCCGA
GAGTATAACA TGGACCTCTA TGTTCTGCGC TTAGCAGAGG ACGGTCCTGC GGAGAGGTGT
GGAAAGATGA GGATTGGTGA TGAAATTTTA GAGATCAATG GTGAGACCAC CAAAAACATG
AAGCATTCTC GAGCTATAGA ACTGATTAAG AATGGTGGCC GCAGAGTTCG TCTGTTTCTG
AAGCGGGGAG ACGGCTCAGT ACCAGAATAT GACCCCAGCA GCGACCGCCA CGGCCCCGCC
ACCGGTCCAC AAGGTGTTCC GGAAGTGAGG GCCGGGCCCG ACCGCCGGCA GCATCCGTCA
TTGGAGTCCA GTTACCCACC CGATCTTCAC AAATCATCAC CACATGGGGA AAAGCGGGCA
CACGCAAGGG ATCCGAAAGG CAGCAGAGAG TATAGCAGAC AACCCAATGA ACACCACACC
TGGAATGGGA CTTCGAGGAA ACCCGACAGC GGGGCATGCC GACCCAAGGA CCGGGCGCCG
GAGGGACGGA GGGACGCGCA GGCCGAGCGC GCGGCAGCCG CCAACGGCCC CAAGAGGCGG
TCCCCAGAGA AGCGGAGGGA GGGCACCCGC AGCGCCGACA ACACTTTGGA GAGGAGGGAG
AAGCACGAGA AGAGACGAGA CGTCTCTCCG GAGCGGAGGC GAGAGCGGTC ACCCACCCGC
AGGAGAGACG GCTCCCCCAG CCGGCGGAGA CGGTCTCTGG AGAGACTCCT GGAGCAGAGG
AGGTCCCCCG AGCGCAGGAG AGGGGGCTCG CCCGAGCGCA GGGCCAAGTC CACCGACCGG
AGGCGCGCAC GCTCCCCCGA GCGCAGGAGA GAGCGGTCCC TGGACAAAAG GAACAGAGAG
GACAGAGCCA GCCACCGAGA AAGGGAAGAG GCGAATCTGA AACAGGATGC CGGCAGAAGT
TCCAGACATC CCCCGGAGCA GAGAAGGCGA CCTTACAAAG AATGTAGCAC CGACCTCAGT
ATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265622.1
CDS529..4974
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1410(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1438..1527(+)
Misc Feature(6)1480..1515(+)
Misc Feature(7)1618..1710(+)
Misc Feature(8)1660..1695(+)
Misc Feature(9)1960..2163(+)
Misc Feature(10)1975..2154(+)
Misc Feature(11)2455..2694(+)
Misc Feature(12)2488..2655(+)
Misc Feature(13)3073..3300(+)
Misc Feature(14)3082..3261(+)
Misc Feature(15)3520..3771(+)
Misc Feature(16)3556..3738(+)
Misc Feature(17)3949..4182(+)
Misc Feature(18)3979..4158(+)
Translation

Target ORF information:

RefSeq Version XM_005265565
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265565

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACCTATGTCA CCTTCACCTG CGTCGGGATT GAGCAAGGGT
GAGAGAGAGA GAGAAATCAA TTCCACGAAT TTTGGAGAAT GTCCGATTCC AGATTACCAG
GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACTGGATTTG GATTTAGGAT TCTGGGTGGA
AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACT
GACGGCCGCC TGAGGTCTGG AGATGAATTA ATCTGTGTGG ATGGGACGCC AGTAATTGGA
AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCTG CCAAGCAAGG CCACGTCAAT
CTCACGGTGC GGCGTAAAGT GGTTTTTGCG GTGCCCAAAA CCGAGAACGA GGTGCCCTCG
CCAGCCTCCT CTCATCACAG TAGCAACCAG CCGGCCTCGC TGACAGAAGA GAAGCGCACT
CCGCAGGGCA GCCAGAACTC GCTGAACACG GTGAGCTCGG GCAGCGGCAG CACCAGCGGC
ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC GTGGTCAGCA CCGTGGTGCA GCCCTACGAC
GTGGAGATCC GGCGCGGGGA GAACGAGGGC TTCGGCTTCG TCATCGTGTC CTCGGTGAGC
AGGCCCGAAG CAGGCACGAC CTTTGGTCGG ATTATTGAGG GGAGCCCTGC TGACCGCTGT
GGCAAGCTGA AAGTAGGAGA CCGGATCTTG GCAGTAAATG GATGTTCCAT CACCAACAAA
TCCCATTCAG ACATTGTGAA CCTAATCAAG GAAGCGGGAA ACACAGTTAC CCTCCGCATC
ATTCCTGGGG ATGAGTCCTC GAATGCCACC TTGCTGACCA ATGCAGAGAA GATTGCCACC
ATCACCACCA CACACACCCC TTCTCAGCAA GGGACCCAGG AGACAAGGAA TACCACCAAA
CCAAAGCAGG AATCTCAATT TGAGTTCAAA GCACCCCAAG CAACACAGGA GCAAGATTTT
TACACTGTGG AACTGGAAAG AGGAGCCAAG GGATTTGGCT TTAGCCTTCG AGGAGGCCGA
GAGTATAACA TGGACCTCTA TGTTCTGCGC TTAGCAGAGG ACGGTCCTGC GGAGAGGTGT
GGAAAGATGA GGATTGGTGA TGAAATTTTA GAGATCAATG GTGAGACCAC CAAAAACATG
AAGCATTCTC GAGCTATAGA ACTGATTAAG AATGGTGGCC GCAGAGTTCG TCTGTTTCTG
AAGCGGGGAG ACGGCTCAGT ACCAGAATAT GACCCCAGCA GCGACCGCCA CGGCCCCGCC
ACCGGTCCAC AAGGTGTTCC GGAAGTGAGG GCCGGGCCCG ACCGCCGGCA GCATCCGTCA
TTGGAGTCCA GTTACCCACC CGATCTTCAC AAATCATCAC CACATGGGGA AAAGCGGGCA
CACGCAAGGG ATCCGAAAGG CAGCAGAGAG TATAGCAGAC AACCCAATGA ACACCACACC
TGGAATGGGA CTTCGAGGAA ACCCGACAGC GGGGCATGCC GACCCAAGGA CCGGGCGCCG
GAGGGACGGA GGGACGCGCA GGCCGAGCGC GCGGCAGCCG CCAACGGCCC CAAGAGGCGG
TCCCCAGAGA AGCGGAGGGA GGGCACCCGC AGCGCCGACA ACACTTTGGA GAGGAGGGAG
AAGCACGAGA AGAGACGAGA CGTCTCTCCG GAGCGGAGGC GAGAGCGGTC ACCCACCCGC
AGGAGAGACG GCTCCCCCAG CCGGCGGAGA CGGTCTCTGG AGAGACTCCT GGAGCAGAGG
AGGTCCCCCG AGCGCAGGAG AGGGGGCTCG CCCGAGCGCA GGGCCAAGTC CACCGACCGG
AGGCGCGCAC GCTCCCCCGA GCGCAGGAGA GAGCGGTCCC TGGACAAAAG GAACAGAGAG
GACAGAGCCA GCCACCGAGA AAGGGAAGAG GCGAATCTGA AACAGGATGC CGGCAGAAGT
TCCAGACATC CCCCGGAGCA GAGAAGGCGA CCTTACAAAG AATGTAGCAC CGACCTCAGT
ATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62631
Clone ID Related Accession (Same CDS sequence) XM_011534236
Accession Version XM_011534236.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAACTG CCTGCTGGTT GGGAAAAGAT TGAAGACCCT
GTCTATGGTA TCTACTATGT AGACCACATC AACAGGAAGA CACAATATGA GAACCCGGTT
CTAGAAGCCA AACGGAAGAA GCAGCTTGAG CAGCAGCAGC AGCAGCAACA GCAGCAGCAA
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG ACAGAAGAAT GGACAGAAGA TCACTCAGCC
CTTGTGCCTC CTGTTATTCC AAACCACCCT CCAAGCAATC CAGAGCCAGC CAGAGAAGTT
CCACTTCAGG GCAAACCCTT TTTTACACGA AACCCTTCTG AGTTGAAAGG CAAGTTCATT
CACACAAAGC TGCGGAAAAG CAGTCGTGGC TTTGGCTTCA CGGTGGTTGG AGGGGATGAA
CCTGATGAGT TTCTCCAGAT CAAGAGCTTG GTCCTAGATG GTCCTGCTGC ATTGGATGGC
AAGATGGAAA CAGGGGATGT GATTGTAAGT GTGAATGACA CCTGTGTTTT GGGACACACA
CATGCTCAAG TTGTGAAGAT CTTCCAGTCC ATCCCCATTG GTGCCAGCGT GGACCTTGAA
CTCTGCCGAG GTTATCCATT GCCTTTTGAT CCAGATGACC CCAATACAAG TTTAGTGACC
TCGGTAGCCA TTTTGGATAA AGAACCAATT ATTGTGAATG GGCAAGAGAC CTATGATTCA
CCAGCTAGCC ACAGTAGTAA AACAGGCAAA GTCAATGGCA TGAAGGATGC CAGGCCAAGC
AGCCCAGCGG ACGTGGCTTC AAATAGTTCT CATGGTTATC CTAATGACAC TGTTTCTTTG
GCTTCCTCCA TAGCCACTCA GCCAGAACTC ATAACTGTTC ATATTGTCAA AGGGCCAATG
GGCTTTGGTT TTACTATCGC AGACAGTCCT GGTGGGGGTG GCCAAAGAGT GAAACAGATT
GTTGACAGCC CAAGGTGCCG AGGCCTGAAA GAAGGGGATC TCATAGTGGA AGTTAATAAG
AAGAACGTGC AGGCCCTAAC TCACAACCAA GTGGTGGATA TGCTGGTTGA GTGTCCTAAG
GGAAGTGAGG TCACATTGTT GGTGCAACGA GGAGGGCTGC CAGTTCCCAA GAAGAGCCCA
AAGTCGCAAC CACTGGAAAG GAAAGACAGC CAGAATAGTT CTCAGCACAG TGTTTCCAGC
CACCGAAGCC TGCACACAGC ATCCCCAAGC CACAGCACAC AGGTGCTCCC CGAGTTCCCA
CCTGCAGAGG CCCAAGCTCC AGATCAAACT GACAGCTCTG GCCAGAAAAA ACCAGATCCT
TTTAAAATCT GGGCCCAGTC CAGGAGCATG TATGAAAACC GACCTATGTC ACCTTCACCT
GCGTCGGGAT TGAGCAAGGG TGAGAGAGAG AGAGAAATCA ATTCCACGAA TTTTGGAGAA
TGTCCGATTC CAGATTACCA GGAACAGGAC ATCTTCCTCT GGAGAAAAGA GACTGGATTT
GGATTTAGGA TTCTGGGTGG AAATGAACCA GGGGAACCTA TTTATATTGG TCACATCGTA
CCACTGGGTG CTGCTGATAC TGACGGCCGC CTGAGGTCTG GAGATGAATT AATCTGTGTG
GATGGGACGC CAGTAATTGG AAAATCACAC CAGCTTGTGG TCCAGCTTAT GCAACAAGCT
GCCAAGCAAG GCCACGTCAA TCTCACGGTG CGGCGTAAAG TGGTTTTTGC GGTGCCCAAA
ACCGAGAACG AGGTGCCCTC GCCAGCCTCC TCTCATCACA GTAGCAACCA GCCGGCCTCG
CTGACAGAAG AGAAGCGCAC TCCGCAGGGC AGCCAGAACT CGCTGAACAC GGTGAGCTCG
GGCAGCGGCA GCACCAGCGG CATCGGCAGT GGCGGCGGCG GGGGCAGCGG CGTGGTCAGC
ACCGTGGTGC AGCCCTACGA CGTGGAGATC CGGCGCGGGG AGAACGAGGG CTTCGGCTTC
GTCATCGTGT CCTCGGTGAG CAGGCCCGAA GCAGGCACGA CCTTTGCAGG CAATGCATGT
GTGGCTATGC CTCACAAAAT AGGTCGGATT ATTGAGGGGA GCCCTGCTGA CCGCTGTGGC
AAGCTGAAAG TAGGAGACCG GATCTTGGCA GTAAATGGAT GTTCCATCAC CAACAAATCC
CATTCAGACA TTGTGAACCT AATCAAGGAA GCGGGAAACA CAGTTACCCT CCGCATCATT
CCTGGGGATG AGTCCTCGAA TGCCACCTTG CTGACCAATG CAGAGAAGAT TGCCACCATC
ACCACCACAC ACACCCCTTC TCAGCAAGGG ACCCAGGAGA CAAGGAATAC CACCAAACCA
AAGCAGGAAT CTCAATTTGA GTTCAAAGCA CCCCAAGCAA CACAGGAGCA AGATTTTTAC
ACTGTGGAAC TGGAAAGAGG AGCCAAGGGA TTTGGCTTTA GCCTTCGAGG AGGCCGAGAG
TATAACATGG ACCTCTATGT TCTGCGCTTA GCAGAGGACG GTCCTGCGGA GAGGTGTGGA
AAGATGAGGA TTGGTGATGA AATTTTAGAG ATCAATGGTG AGACCACCAA AAACATGAAG
CATTCTCGAG CTATAGAACT GATTAAGAAT GGTGGCCGCA GAGTTCGTCT GTTTCTGAAG
CGGGGAGACG GCTCAGTACC AGAATATGAC CCCAGCAGCG ACCGCCACGG CCCCGCCACC
GGTCCACAAG GTGTTCCGGA AGTGAGGGCC GGGCCCGACC GCCGGCAGCA TCCGTCATTG
GAGTCCAGTT ACCCACCCGA TCTTCACAAA TCATCACCAC ATGGGGAAAA GCGGGCACAC
GCAAGGGATC CGAAAGGCAG CAGAGAGTAT AGCAGACAAC CCAATGAACA CCACACCTGG
AATGGGACTT CGAGGAAACC CGACAGCGGG GCATGCCGAC CCAAGGACCG GGCGCCGGAG
GGACGGAGGG ACGCGCAGGC CGAGCGCGCG GCAGCCGCCA ACGGCCCCAA GAGGCGGTCC
CCAGAGAAGC GGAGGGAGGG CACCCGCAGC GCCGACAACA CTTTGGAGAG GAGGGAGAAG
CACGAGAAGA GACGAGACGT CTCTCCGGAG CGGAGGCGAG AGCGGTCACC CACCCGCAGG
AGAGACGGCT CCCCCAGCCG GCGGAGACGG TCTCTGGAGA GACTCCTGGA GCAGAGGAGG
TCCCCCGAGC GCAGGAGAGG GGGCTCGCCC GAGCGCAGGG CCAAGTCCAC CGACCGGAGG
CGCGCACGCT CCCCCGAGCG CAGGAGAGAG CGGTCCCTGG ACAAAAGGAA CAGAGAGGAC
AGAGCCAGCC ACCGAGAAAG GGAAGAGGCG AATCTGAAAC AGGATGCCGG CAGAAGTTCC
AGACATCCCC CGGAGCAGAG AAGGCGACCT TACAAAGAAT GTAGCACCGA CCTCAGTATC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532538.1
CDS529..4971
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1410(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1438..1527(+)
Misc Feature(6)1480..1515(+)
Misc Feature(7)1579..1671(+)
Misc Feature(8)1621..1656(+)
Misc Feature(9)1921..2124(+)
Misc Feature(10)1936..2115(+)
Misc Feature(11)2416..2655(+)
Misc Feature(12)2449..2616(+)
Misc Feature(13)3034..3261(+)
Misc Feature(14)3043..3222(+)
Misc Feature(15)3481..3768(+)
Misc Feature(16)3517..3735(+)
Misc Feature(17)3946..4179(+)
Misc Feature(18)3976..4155(+)
Translation

Target ORF information:

RefSeq Version XM_011534236
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534236

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAACTG CCTGCTGGTT GGGAAAAGAT TGAAGACCCT
GTCTATGGTA TCTACTATGT AGACCACATC AACAGGAAGA CACAATATGA GAACCCGGTT
CTAGAAGCCA AACGGAAGAA GCAGCTTGAG CAGCAGCAGC AGCAGCAACA GCAGCAGCAA
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG ACAGAAGAAT GGACAGAAGA TCACTCAGCC
CTTGTGCCTC CTGTTATTCC AAACCACCCT CCAAGCAATC CAGAGCCAGC CAGAGAAGTT
CCACTTCAGG GCAAACCCTT TTTTACACGA AACCCTTCTG AGTTGAAAGG CAAGTTCATT
CACACAAAGC TGCGGAAAAG CAGTCGTGGC TTTGGCTTCA CGGTGGTTGG AGGGGATGAA
CCTGATGAGT TTCTCCAGAT CAAGAGCTTG GTCCTAGATG GTCCTGCTGC ATTGGATGGC
AAGATGGAAA CAGGGGATGT GATTGTAAGT GTGAATGACA CCTGTGTTTT GGGACACACA
CATGCTCAAG TTGTGAAGAT CTTCCAGTCC ATCCCCATTG GTGCCAGCGT GGACCTTGAA
CTCTGCCGAG GTTATCCATT GCCTTTTGAT CCAGATGACC CCAATACAAG TTTAGTGACC
TCGGTAGCCA TTTTGGATAA AGAACCAATT ATTGTGAATG GGCAAGAGAC CTATGATTCA
CCAGCTAGCC ACAGTAGTAA AACAGGCAAA GTCAATGGCA TGAAGGATGC CAGGCCAAGC
AGCCCAGCGG ACGTGGCTTC AAATAGTTCT CATGGTTATC CTAATGACAC TGTTTCTTTG
GCTTCCTCCA TAGCCACTCA GCCAGAACTC ATAACTGTTC ATATTGTCAA AGGGCCAATG
GGCTTTGGTT TTACTATCGC AGACAGTCCT GGTGGGGGTG GCCAAAGAGT GAAACAGATT
GTTGACAGCC CAAGGTGCCG AGGCCTGAAA GAAGGGGATC TCATAGTGGA AGTTAATAAG
AAGAACGTGC AGGCCCTAAC TCACAACCAA GTGGTGGATA TGCTGGTTGA GTGTCCTAAG
GGAAGTGAGG TCACATTGTT GGTGCAACGA GGAGGGCTGC CAGTTCCCAA GAAGAGCCCA
AAGTCGCAAC CACTGGAAAG GAAAGACAGC CAGAATAGTT CTCAGCACAG TGTTTCCAGC
CACCGAAGCC TGCACACAGC ATCCCCAAGC CACAGCACAC AGGTGCTCCC CGAGTTCCCA
CCTGCAGAGG CCCAAGCTCC AGATCAAACT GACAGCTCTG GCCAGAAAAA ACCAGATCCT
TTTAAAATCT GGGCCCAGTC CAGGAGCATG TATGAAAACC GACCTATGTC ACCTTCACCT
GCGTCGGGAT TGAGCAAGGG TGAGAGAGAG AGAGAAATCA ATTCCACGAA TTTTGGAGAA
TGTCCGATTC CAGATTACCA GGAACAGGAC ATCTTCCTCT GGAGAAAAGA GACTGGATTT
GGATTTAGGA TTCTGGGTGG AAATGAACCA GGGGAACCTA TTTATATTGG TCACATCGTA
CCACTGGGTG CTGCTGATAC TGACGGCCGC CTGAGGTCTG GAGATGAATT AATCTGTGTG
GATGGGACGC CAGTAATTGG AAAATCACAC CAGCTTGTGG TCCAGCTTAT GCAACAAGCT
GCCAAGCAAG GCCACGTCAA TCTCACGGTG CGGCGTAAAG TGGTTTTTGC GGTGCCCAAA
ACCGAGAACG AGGTGCCCTC GCCAGCCTCC TCTCATCACA GTAGCAACCA GCCGGCCTCG
CTGACAGAAG AGAAGCGCAC TCCGCAGGGC AGCCAGAACT CGCTGAACAC GGTGAGCTCG
GGCAGCGGCA GCACCAGCGG CATCGGCAGT GGCGGCGGCG GGGGCAGCGG CGTGGTCAGC
ACCGTGGTGC AGCCCTACGA CGTGGAGATC CGGCGCGGGG AGAACGAGGG CTTCGGCTTC
GTCATCGTGT CCTCGGTGAG CAGGCCCGAA GCAGGCACGA CCTTTGCAGG CAATGCATGT
GTGGCTATGC CTCACAAAAT AGGTCGGATT ATTGAGGGGA GCCCTGCTGA CCGCTGTGGC
AAGCTGAAAG TAGGAGACCG GATCTTGGCA GTAAATGGAT GTTCCATCAC CAACAAATCC
CATTCAGACA TTGTGAACCT AATCAAGGAA GCGGGAAACA CAGTTACCCT CCGCATCATT
CCTGGGGATG AGTCCTCGAA TGCCACCTTG CTGACCAATG CAGAGAAGAT TGCCACCATC
ACCACCACAC ACACCCCTTC TCAGCAAGGG ACCCAGGAGA CAAGGAATAC CACCAAACCA
AAGCAGGAAT CTCAATTTGA GTTCAAAGCA CCCCAAGCAA CACAGGAGCA AGATTTTTAC
ACTGTGGAAC TGGAAAGAGG AGCCAAGGGA TTTGGCTTTA GCCTTCGAGG AGGCCGAGAG
TATAACATGG ACCTCTATGT TCTGCGCTTA GCAGAGGACG GTCCTGCGGA GAGGTGTGGA
AAGATGAGGA TTGGTGATGA AATTTTAGAG ATCAATGGTG AGACCACCAA AAACATGAAG
CATTCTCGAG CTATAGAACT GATTAAGAAT GGTGGCCGCA GAGTTCGTCT GTTTCTGAAG
CGGGGAGACG GCTCAGTACC AGAATATGAC CCCAGCAGCG ACCGCCACGG CCCCGCCACC
GGTCCACAAG GTGTTCCGGA AGTGAGGGCC GGGCCCGACC GCCGGCAGCA TCCGTCATTG
GAGTCCAGTT ACCCACCCGA TCTTCACAAA TCATCACCAC ATGGGGAAAA GCGGGCACAC
GCAAGGGATC CGAAAGGCAG CAGAGAGTAT AGCAGACAAC CCAATGAACA CCACACCTGG
AATGGGACTT CGAGGAAACC CGACAGCGGG GCATGCCGAC CCAAGGACCG GGCGCCGGAG
GGACGGAGGG ACGCGCAGGC CGAGCGCGCG GCAGCCGCCA ACGGCCCCAA GAGGCGGTCC
CCAGAGAAGC GGAGGGAGGG CACCCGCAGC GCCGACAACA CTTTGGAGAG GAGGGAGAAG
CACGAGAAGA GACGAGACGT CTCTCCGGAG CGGAGGCGAG AGCGGTCACC CACCCGCAGG
AGAGACGGCT CCCCCAGCCG GCGGAGACGG TCTCTGGAGA GACTCCTGGA GCAGAGGAGG
TCCCCCGAGC GCAGGAGAGG GGGCTCGCCC GAGCGCAGGG CCAAGTCCAC CGACCGGAGG
CGCGCACGCT CCCCCGAGCG CAGGAGAGAG CGGTCCCTGG ACAAAAGGAA CAGAGAGGAC
AGAGCCAGCC ACCGAGAAAG GGAAGAGGCG AATCTGAAAC AGGATGCCGG CAGAAGTTCC
AGACATCCCC CGGAGCAGAG AAGGCGACCT TACAAAGAAT GTAGCACCGA CCTCAGTATC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37589
Clone ID Related Accession (Same CDS sequence) XM_005265566
Accession Version XM_005265566.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4440bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACCTAT GTCACCTTCA CCTGCGTCGG GATTGAGCAA GGGTGAGAGA
GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCCGA TTCCAGATTA CCAGGAACAG
GACATCTTCC TCTGGAGAAA AGAGACTGGA TTTGGATTTA GGATTCTGGG TGGAAATGAA
CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACTGACGGC
CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA CGCCAGTAAT TGGAAAATCA
CACCAGCTTG TGGTCCAGCT TATGCAACAA GCTGCCAAGC AAGGCCACGT CAATCTCACG
GTGCGGCGTA AAGTGGTTTT TGCGGTGCCC AAAACCGAGA ACGAGGTGCC CTCGCCAGCC
TCCTCTCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACAG AAGAGAAGCG CACTCCGCAG
GGCAGCCAGA ACTCGCTGAA CACGGTGAGC TCGGGCAGCG GCAGCACCAG CGGCATCGGC
AGTGGCGGCG GCGGGGGCAG CGGCGTGGTC AGCACCGTGG TGCAGCCCTA CGACGTGGAG
ATCCGGCGCG GGGAGAACGA GGGCTTCGGC TTCGTCATCG TGTCCTCGGT GAGCAGGCCC
GAAGCAGGCA CGACCTTTGG TCGGATTATT GAGGGGAGCC CTGCTGACCG CTGTGGCAAG
CTGAAAGTAG GAGACCGGAT CTTGGCAGTA AATGGATGTT CCATCACCAA CAAATCCCAT
TCAGACATTG TGAACCTAAT CAAGGAAGCG GGAAACACAG TTACCCTCCG CATCATTCCT
GGGGATGAGT CCTCGAATGC CACCTTGCTG ACCAATGCAG AGAAGATTGC CACCATCACC
ACCACACACA CCCCTTCTCA GCAAGGGACC CAGGAGACAA GGAATACCAC CAAACCAAAG
CAGGAATCTC AATTTGAGTT CAAAGCACCC CAAGCAACAC AGGAGCAAGA TTTTTACACT
GTGGAACTGG AAAGAGGAGC CAAGGGATTT GGCTTTAGCC TTCGAGGAGG CCGAGAGTAT
AACATGGACC TCTATGTTCT GCGCTTAGCA GAGGACGGTC CTGCGGAGAG GTGTGGAAAG
ATGAGGATTG GTGATGAAAT TTTAGAGATC AATGGTGAGA CCACCAAAAA CATGAAGCAT
TCTCGAGCTA TAGAACTGAT TAAGAATGGT GGCCGCAGAG TTCGTCTGTT TCTGAAGCGG
GGAGACGGCT CAGTACCAGA ATATGACCCC AGCAGCGACC GCCACGGCCC CGCCACCGGT
CCACAAGGTG TTCCGGAAGT GAGGGCCGGG CCCGACCGCC GGCAGCATCC GTCATTGGAG
TCCAGTTACC CACCCGATCT TCACAAATCA TCACCACATG GGGAAAAGCG GGCACACGCA
AGGGATCCGA AAGGCAGCAG AGAGTATAGC AGACAACCCA ATGAACACCA CACCTGGAAT
GGGACTTCGA GGAAACCCGA CAGCGGGGCA TGCCGACCCA AGGACCGGGC GCCGGAGGGA
CGGAGGGACG CGCAGGCCGA GCGCGCGGCA GCCGCCAACG GCCCCAAGAG GCGGTCCCCA
GAGAAGCGGA GGGAGGGCAC CCGCAGCGCC GACAACACTT TGGAGAGGAG GGAGAAGCAC
GAGAAGAGAC GAGACGTCTC TCCGGAGCGG AGGCGAGAGC GGTCACCCAC CCGCAGGAGA
GACGGCTCCC CCAGCCGGCG GAGACGGTCT CTGGAGAGAC TCCTGGAGCA GAGGAGGTCC
CCCGAGCGCA GGAGAGGGGG CTCGCCCGAG CGCAGGGCCA AGTCCACCGA CCGGAGGCGC
GCACGCTCCC CCGAGCGCAG GAGAGAGCGG TCCCTGGACA AAAGGAACAG AGAGGACAGA
GCCAGCCACC GAGAAAGGGA AGAGGCGAAT CTGAAACAGG ATGCCGGCAG AAGTTCCAGA
CATCCCCCGG AGCAGAGAAG GCGACCTTAC AAAGAATGTA GCACCGACCT CAGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265623.1
CDS529..4968
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1407(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1435..1524(+)
Misc Feature(6)1477..1512(+)
Misc Feature(7)1612..1704(+)
Misc Feature(8)1654..1689(+)
Misc Feature(9)1954..2157(+)
Misc Feature(10)1969..2148(+)
Misc Feature(11)2449..2688(+)
Misc Feature(12)2482..2649(+)
Misc Feature(13)3067..3294(+)
Misc Feature(14)3076..3255(+)
Misc Feature(15)3514..3765(+)
Misc Feature(16)3550..3732(+)
Misc Feature(17)3943..4176(+)
Misc Feature(18)3973..4152(+)
Translation

Target ORF information:

RefSeq Version XM_005265566
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265566

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACCTAT GTCACCTTCA CCTGCGTCGG GATTGAGCAA GGGTGAGAGA
GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCCGA TTCCAGATTA CCAGGAACAG
GACATCTTCC TCTGGAGAAA AGAGACTGGA TTTGGATTTA GGATTCTGGG TGGAAATGAA
CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACTGACGGC
CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA CGCCAGTAAT TGGAAAATCA
CACCAGCTTG TGGTCCAGCT TATGCAACAA GCTGCCAAGC AAGGCCACGT CAATCTCACG
GTGCGGCGTA AAGTGGTTTT TGCGGTGCCC AAAACCGAGA ACGAGGTGCC CTCGCCAGCC
TCCTCTCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACAG AAGAGAAGCG CACTCCGCAG
GGCAGCCAGA ACTCGCTGAA CACGGTGAGC TCGGGCAGCG GCAGCACCAG CGGCATCGGC
AGTGGCGGCG GCGGGGGCAG CGGCGTGGTC AGCACCGTGG TGCAGCCCTA CGACGTGGAG
ATCCGGCGCG GGGAGAACGA GGGCTTCGGC TTCGTCATCG TGTCCTCGGT GAGCAGGCCC
GAAGCAGGCA CGACCTTTGG TCGGATTATT GAGGGGAGCC CTGCTGACCG CTGTGGCAAG
CTGAAAGTAG GAGACCGGAT CTTGGCAGTA AATGGATGTT CCATCACCAA CAAATCCCAT
TCAGACATTG TGAACCTAAT CAAGGAAGCG GGAAACACAG TTACCCTCCG CATCATTCCT
GGGGATGAGT CCTCGAATGC CACCTTGCTG ACCAATGCAG AGAAGATTGC CACCATCACC
ACCACACACA CCCCTTCTCA GCAAGGGACC CAGGAGACAA GGAATACCAC CAAACCAAAG
CAGGAATCTC AATTTGAGTT CAAAGCACCC CAAGCAACAC AGGAGCAAGA TTTTTACACT
GTGGAACTGG AAAGAGGAGC CAAGGGATTT GGCTTTAGCC TTCGAGGAGG CCGAGAGTAT
AACATGGACC TCTATGTTCT GCGCTTAGCA GAGGACGGTC CTGCGGAGAG GTGTGGAAAG
ATGAGGATTG GTGATGAAAT TTTAGAGATC AATGGTGAGA CCACCAAAAA CATGAAGCAT
TCTCGAGCTA TAGAACTGAT TAAGAATGGT GGCCGCAGAG TTCGTCTGTT TCTGAAGCGG
GGAGACGGCT CAGTACCAGA ATATGACCCC AGCAGCGACC GCCACGGCCC CGCCACCGGT
CCACAAGGTG TTCCGGAAGT GAGGGCCGGG CCCGACCGCC GGCAGCATCC GTCATTGGAG
TCCAGTTACC CACCCGATCT TCACAAATCA TCACCACATG GGGAAAAGCG GGCACACGCA
AGGGATCCGA AAGGCAGCAG AGAGTATAGC AGACAACCCA ATGAACACCA CACCTGGAAT
GGGACTTCGA GGAAACCCGA CAGCGGGGCA TGCCGACCCA AGGACCGGGC GCCGGAGGGA
CGGAGGGACG CGCAGGCCGA GCGCGCGGCA GCCGCCAACG GCCCCAAGAG GCGGTCCCCA
GAGAAGCGGA GGGAGGGCAC CCGCAGCGCC GACAACACTT TGGAGAGGAG GGAGAAGCAC
GAGAAGAGAC GAGACGTCTC TCCGGAGCGG AGGCGAGAGC GGTCACCCAC CCGCAGGAGA
GACGGCTCCC CCAGCCGGCG GAGACGGTCT CTGGAGAGAC TCCTGGAGCA GAGGAGGTCC
CCCGAGCGCA GGAGAGGGGG CTCGCCCGAG CGCAGGGCCA AGTCCACCGA CCGGAGGCGC
GCACGCTCCC CCGAGCGCAG GAGAGAGCGG TCCCTGGACA AAAGGAACAG AGAGGACAGA
GCCAGCCACC GAGAAAGGGA AGAGGCGAAT CTGAAACAGG ATGCCGGCAG AAGTTCCAGA
CATCCCCCGG AGCAGAGAAG GCGACCTTAC AAAGAATGTA GCACCGACCT CAGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37590
Clone ID Related Accession (Same CDS sequence) XM_006713410
Accession Version XM_006713410.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4440bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCGGTTCTA
GAAGCCAAAC GGAAGAAGCA GCTTGAGCAG CAGCAGCAGC AGCAACAGCA GCAGCAACAG
CAGCAGCAGC AGCAGCAGCA GCAGCAGACA GAAGAATGGA CAGAAGATCA CTCAGCCCTT
GTGCCTCCTG TTATTCCAAA CCACCCTCCA AGCAATCCAG AGCCAGCCAG AGAAGTTCCA
CTTCAGGGCA AACCCTTTTT TACACGAAAC CCTTCTGAGT TGAAAGGCAA GTTCATTCAC
ACAAAGCTGC GGAAAAGCAG TCGTGGCTTT GGCTTCACGG TGGTTGGAGG GGATGAACCT
GATGAGTTTC TCCAGATCAA GAGCTTGGTC CTAGATGGTC CTGCTGCATT GGATGGCAAG
ATGGAAACAG GGGATGTGAT TGTAAGTGTG AATGACACCT GTGTTTTGGG ACACACACAT
GCTCAAGTTG TGAAGATCTT CCAGTCCATC CCCATTGGTG CCAGCGTGGA CCTTGAACTC
TGCCGAGGTT ATCCATTGCC TTTTGATCCA GATGACCCCA ATACAAGTTT AGTGACCTCG
GTAGCCATTT TGGATAAAGA ACCAATTATT GTGAATGGGC AAGAGACCTA TGATTCACCA
GCTAGCCACA GTAGTAAAAC AGGCAAAGTC AATGGCATGA AGGATGCCAG GCCAAGCAGC
CCAGCGGACG TGGCTTCAAA TAGTTCTCAT GGTTATCCTA ATGACACTGT TTCTTTGGCT
TCCTCCATAG CCACTCAGCC AGAACTCATA ACTGTTCATA TTGTCAAAGG GCCAATGGGC
TTTGGTTTTA CTATCGCAGA CAGTCCTGGT GGGGGTGGCC AAAGAGTGAA ACAGATTGTT
GACAGCCCAA GGTGCCGAGG CCTGAAAGAA GGGGATCTCA TAGTGGAAGT TAATAAGAAG
AACGTGCAGG CCCTAACTCA CAACCAAGTG GTGGATATGC TGGTTGAGTG TCCTAAGGGA
AGTGAGGTCA CATTGTTGGT GCAACGAGGA GGGCTGCCAG TTCCCAAGAA GAGCCCAAAG
TCGCAACCAC TGGAAAGGAA AGACAGCCAG AATAGTTCTC AGCACAGTGT TTCCAGCCAC
CGAAGCCTGC ACACAGCATC CCCAAGCCAC AGCACACAGG TGCTCCCCGA GTTCCCACCT
GCAGAGGCCC AAGCTCCAGA TCAAACTGAC AGCTCTGGCC AGAAAAAACC AGATCCTTTT
AAAATCTGGG CCCAGTCCAG GAGCATGTAT GAAAACCGAC CTATGTCACC TTCACCTGCG
TCGGGATTGA GCAAGGGTGA GAGAGAGAGA GAAATCAATT CCACGAATTT TGGAGAATGT
CCGATTCCAG ATTACCAGGA ACAGGACATC TTCCTCTGGA GAAAAGAGAC TGGATTTGGA
TTTAGGATTC TGGGTGGAAA TGAACCAGGG GAACCTATTT ATATTGGTCA CATCGTACCA
CTGGGTGCTG CTGATACTGA CGGCCGCCTG AGGTCTGGAG ATGAATTAAT CTGTGTGGAT
GGGACGCCAG TAATTGGAAA ATCACACCAG CTTGTGGTCC AGCTTATGCA ACAAGCTGCC
AAGCAAGGCC ACGTCAATCT CACGGTGCGG CGTAAAGTGG TTTTTGCGGT GCCCAAAACC
GAGAACGAGG TGCCCTCGCC AGCCTCCTCT CATCACAGTA GCAACCAGCC GGCCTCGCTG
ACAGAAGAGA AGCGCACTCC GCAGGGCAGC CAGAACTCGC TGAACACGGT GAGCTCGGGC
AGCGGCAGCA CCAGCGGCAT CGGCAGTGGC GGCGGCGGGG GCAGCGGCGT GGTCAGCACC
GTGGTGCAGC CCTACGACGT GGAGATCCGG CGCGGGGAGA ACGAGGGCTT CGGCTTCGTC
ATCGTGTCCT CGGTGAGCAG GCCCGAAGCA GGCACGACCT TTGCAGGCAA TGCATGTGTG
GCTATGCCTC ACAAAATAGG TCGGATTATT GAGGGGAGCC CTGCTGACCG CTGTGGCAAG
CTGAAAGTAG GAGACCGGAT CTTGGCAGTA AATGGATGTT CCATCACCAA CAAATCCCAT
TCAGACATTG TGAACCTAAT CAAGGAAGCG GGAAACACAG TTACCCTCCG CATCATTCCT
GGGGATGAGT CCTCGAATGC CACCTTGCTG ACCAATGCAG AGAAGATTGC CACCATCACC
ACCACACACA CCCCTTCTCA GCAAGGGACC CAGGAGACAA GGAATACCAC CAAACCAAAG
CAGGAATCTC AATTTGAGTT CAAAGCACCC CAAGCAACAC AGGAGCAAGA TTTTTACACT
GTGGAACTGG AAAGAGGAGC CAAGGGATTT GGCTTTAGCC TTCGAGGAGG CCGAGAGTAT
AACATGGACC TCTATGTTCT GCGCTTAGCA GAGGACGGTC CTGCGGAGAG GTGTGGAAAG
ATGAGGATTG GTGATGAAAT TTTAGAGATC AATGGTGAGA CCACCAAAAA CATGAAGCAT
TCTCGAGCTA TAGAACTGAT TAAGAATGGT GGCCGCAGAG TTCGTCTGTT TCTGAAGCGG
GGAGACGGCT CAGTACCAGA ATATGACCCC AGCAGCGACC GCCACGGCCC CGCCACCGGT
CCACAAGGTG TTCCGGAAGT GAGGGCCGGG CCCGACCGCC GGCAGCATCC GTCATTGGAG
TCCAGTTACC CACCCGATCT TCACAAATCA TCACCACATG GGGAAAAGCG GGCACACGCA
AGGGATCCGA AAGGCAGCAG AGAGTATAGC AGACAACCCA ATGAACACCA CACCTGGAAT
GGGACTTCGA GGAAACCCGA CAGCGGGGCA TGCCGACCCA AGGACCGGGC GCCGGAGGGA
CGGAGGGACG CGCAGGCCGA GCGCGCGGCA GCCGCCAACG GCCCCAAGAG GCGGTCCCCA
GAGAAGCGGA GGGAGGGCAC CCGCAGCGCC GACAACACTT TGGAGAGGAG GGAGAAGCAC
GAGAAGAGAC GAGACGTCTC TCCGGAGCGG AGGCGAGAGC GGTCACCCAC CCGCAGGAGA
GACGGCTCCC CCAGCCGGCG GAGACGGTCT CTGGAGAGAC TCCTGGAGCA GAGGAGGTCC
CCCGAGCGCA GGAGAGGGGG CTCGCCCGAG CGCAGGGCCA AGTCCACCGA CCGGAGGCGC
GCACGCTCCC CCGAGCGCAG GAGAGAGCGG TCCCTGGACA AAAGGAACAG AGAGGACAGA
GCCAGCCACC GAGAAAGGGA AGAGGCGAAT CTGAAACAGG ATGCCGGCAG AAGTTCCAGA
CATCCCCCGG AGCAGAGAAG GCGACCTTAC AAAGAATGTA GCACCGACCT CAGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713473.1
CDS529..4968
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1407(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1435..1524(+)
Misc Feature(6)1477..1512(+)
Misc Feature(7)1576..1668(+)
Misc Feature(8)1618..1653(+)
Misc Feature(9)1918..2121(+)
Misc Feature(10)1933..2112(+)
Misc Feature(11)2413..2652(+)
Misc Feature(12)2446..2613(+)
Misc Feature(13)3031..3258(+)
Misc Feature(14)3040..3219(+)
Misc Feature(15)3478..3765(+)
Misc Feature(16)3514..3732(+)
Misc Feature(17)3943..4176(+)
Misc Feature(18)3973..4152(+)
Translation

Target ORF information:

RefSeq Version XM_006713410
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006713410

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCGGTTCTA
GAAGCCAAAC GGAAGAAGCA GCTTGAGCAG CAGCAGCAGC AGCAACAGCA GCAGCAACAG
CAGCAGCAGC AGCAGCAGCA GCAGCAGACA GAAGAATGGA CAGAAGATCA CTCAGCCCTT
GTGCCTCCTG TTATTCCAAA CCACCCTCCA AGCAATCCAG AGCCAGCCAG AGAAGTTCCA
CTTCAGGGCA AACCCTTTTT TACACGAAAC CCTTCTGAGT TGAAAGGCAA GTTCATTCAC
ACAAAGCTGC GGAAAAGCAG TCGTGGCTTT GGCTTCACGG TGGTTGGAGG GGATGAACCT
GATGAGTTTC TCCAGATCAA GAGCTTGGTC CTAGATGGTC CTGCTGCATT GGATGGCAAG
ATGGAAACAG GGGATGTGAT TGTAAGTGTG AATGACACCT GTGTTTTGGG ACACACACAT
GCTCAAGTTG TGAAGATCTT CCAGTCCATC CCCATTGGTG CCAGCGTGGA CCTTGAACTC
TGCCGAGGTT ATCCATTGCC TTTTGATCCA GATGACCCCA ATACAAGTTT AGTGACCTCG
GTAGCCATTT TGGATAAAGA ACCAATTATT GTGAATGGGC AAGAGACCTA TGATTCACCA
GCTAGCCACA GTAGTAAAAC AGGCAAAGTC AATGGCATGA AGGATGCCAG GCCAAGCAGC
CCAGCGGACG TGGCTTCAAA TAGTTCTCAT GGTTATCCTA ATGACACTGT TTCTTTGGCT
TCCTCCATAG CCACTCAGCC AGAACTCATA ACTGTTCATA TTGTCAAAGG GCCAATGGGC
TTTGGTTTTA CTATCGCAGA CAGTCCTGGT GGGGGTGGCC AAAGAGTGAA ACAGATTGTT
GACAGCCCAA GGTGCCGAGG CCTGAAAGAA GGGGATCTCA TAGTGGAAGT TAATAAGAAG
AACGTGCAGG CCCTAACTCA CAACCAAGTG GTGGATATGC TGGTTGAGTG TCCTAAGGGA
AGTGAGGTCA CATTGTTGGT GCAACGAGGA GGGCTGCCAG TTCCCAAGAA GAGCCCAAAG
TCGCAACCAC TGGAAAGGAA AGACAGCCAG AATAGTTCTC AGCACAGTGT TTCCAGCCAC
CGAAGCCTGC ACACAGCATC CCCAAGCCAC AGCACACAGG TGCTCCCCGA GTTCCCACCT
GCAGAGGCCC AAGCTCCAGA TCAAACTGAC AGCTCTGGCC AGAAAAAACC AGATCCTTTT
AAAATCTGGG CCCAGTCCAG GAGCATGTAT GAAAACCGAC CTATGTCACC TTCACCTGCG
TCGGGATTGA GCAAGGGTGA GAGAGAGAGA GAAATCAATT CCACGAATTT TGGAGAATGT
CCGATTCCAG ATTACCAGGA ACAGGACATC TTCCTCTGGA GAAAAGAGAC TGGATTTGGA
TTTAGGATTC TGGGTGGAAA TGAACCAGGG GAACCTATTT ATATTGGTCA CATCGTACCA
CTGGGTGCTG CTGATACTGA CGGCCGCCTG AGGTCTGGAG ATGAATTAAT CTGTGTGGAT
GGGACGCCAG TAATTGGAAA ATCACACCAG CTTGTGGTCC AGCTTATGCA ACAAGCTGCC
AAGCAAGGCC ACGTCAATCT CACGGTGCGG CGTAAAGTGG TTTTTGCGGT GCCCAAAACC
GAGAACGAGG TGCCCTCGCC AGCCTCCTCT CATCACAGTA GCAACCAGCC GGCCTCGCTG
ACAGAAGAGA AGCGCACTCC GCAGGGCAGC CAGAACTCGC TGAACACGGT GAGCTCGGGC
AGCGGCAGCA CCAGCGGCAT CGGCAGTGGC GGCGGCGGGG GCAGCGGCGT GGTCAGCACC
GTGGTGCAGC CCTACGACGT GGAGATCCGG CGCGGGGAGA ACGAGGGCTT CGGCTTCGTC
ATCGTGTCCT CGGTGAGCAG GCCCGAAGCA GGCACGACCT TTGCAGGCAA TGCATGTGTG
GCTATGCCTC ACAAAATAGG TCGGATTATT GAGGGGAGCC CTGCTGACCG CTGTGGCAAG
CTGAAAGTAG GAGACCGGAT CTTGGCAGTA AATGGATGTT CCATCACCAA CAAATCCCAT
TCAGACATTG TGAACCTAAT CAAGGAAGCG GGAAACACAG TTACCCTCCG CATCATTCCT
GGGGATGAGT CCTCGAATGC CACCTTGCTG ACCAATGCAG AGAAGATTGC CACCATCACC
ACCACACACA CCCCTTCTCA GCAAGGGACC CAGGAGACAA GGAATACCAC CAAACCAAAG
CAGGAATCTC AATTTGAGTT CAAAGCACCC CAAGCAACAC AGGAGCAAGA TTTTTACACT
GTGGAACTGG AAAGAGGAGC CAAGGGATTT GGCTTTAGCC TTCGAGGAGG CCGAGAGTAT
AACATGGACC TCTATGTTCT GCGCTTAGCA GAGGACGGTC CTGCGGAGAG GTGTGGAAAG
ATGAGGATTG GTGATGAAAT TTTAGAGATC AATGGTGAGA CCACCAAAAA CATGAAGCAT
TCTCGAGCTA TAGAACTGAT TAAGAATGGT GGCCGCAGAG TTCGTCTGTT TCTGAAGCGG
GGAGACGGCT CAGTACCAGA ATATGACCCC AGCAGCGACC GCCACGGCCC CGCCACCGGT
CCACAAGGTG TTCCGGAAGT GAGGGCCGGG CCCGACCGCC GGCAGCATCC GTCATTGGAG
TCCAGTTACC CACCCGATCT TCACAAATCA TCACCACATG GGGAAAAGCG GGCACACGCA
AGGGATCCGA AAGGCAGCAG AGAGTATAGC AGACAACCCA ATGAACACCA CACCTGGAAT
GGGACTTCGA GGAAACCCGA CAGCGGGGCA TGCCGACCCA AGGACCGGGC GCCGGAGGGA
CGGAGGGACG CGCAGGCCGA GCGCGCGGCA GCCGCCAACG GCCCCAAGAG GCGGTCCCCA
GAGAAGCGGA GGGAGGGCAC CCGCAGCGCC GACAACACTT TGGAGAGGAG GGAGAAGCAC
GAGAAGAGAC GAGACGTCTC TCCGGAGCGG AGGCGAGAGC GGTCACCCAC CCGCAGGAGA
GACGGCTCCC CCAGCCGGCG GAGACGGTCT CTGGAGAGAC TCCTGGAGCA GAGGAGGTCC
CCCGAGCGCA GGAGAGGGGG CTCGCCCGAG CGCAGGGCCA AGTCCACCGA CCGGAGGCGC
GCACGCTCCC CCGAGCGCAG GAGAGAGCGG TCCCTGGACA AAAGGAACAG AGAGGACAGA
GCCAGCCACC GAGAAAGGGA AGAGGCGAAT CTGAAACAGG ATGCCGGCAG AAGTTCCAGA
CATCCCCCGG AGCAGAGAAG GCGACCTTAC AAAGAATGTA GCACCGACCT CAGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37591
Clone ID Related Accession (Same CDS sequence) XM_005265568
Accession Version XM_005265568.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCGGTTCTA
GAAGCCAAAC GGAAGAAGCA GCTTGAGCAG CAGCAGCAGC AGCAACAGCA GCAGCAACAG
CAGCAGCAGC AGCAGCAGCA GCAGCAGACA GAAGAATGGA CAGAAGATCA CTCAGCCCTT
GTGCCTCCTG TTATTCCAAA CCACCCTCCA AGCAATCCAG AGCCAGCCAG AGAAGTTCCA
CTTCAGGGCA AACCCTTTTT TACACGAAAC CCTTCTGAGT TGAAAGGCAA GTTCATTCAC
ACAAAGCTGC GGAAAAGCAG TCGTGGCTTT GGCTTCACGG TGGTTGGAGG GGATGAACCT
GATGAGTTTC TCCAGATCAA GAGCTTGGTC CTAGATGGTC CTGCTGCATT GGATGGCAAG
ATGGAAACAG GGGATGTGAT TGTAAGTGTG AATGACACCT GTGTTTTGGG ACACACACAT
GCTCAAGTTG TGAAGATCTT CCAGTCCATC CCCATTGGTG CCAGCGTGGA CCTTGAACTC
TGCCGAGGTT ATCCATTGCC TTTTGATCCA GATGACCCCA ATACAAGTTT AGTGACCTCG
GTAGCCATTT TGGATAAAGA ACCAATTATT GTGAATGGGC AAGAGACCTA TGATTCACCA
GCTAGCCACA GTAGTAAAAC AGGCAAAGTC AATGGCATGA AGGATGCCAG GCCAAGCAGC
CCAGCGGACG TGGCTTCAAA TAGTTCTCAT GGTTATCCTA ATGACACTGT TTCTTTGGCT
TCCTCCATAG CCACTCAGCC AGAACTCATA ACTGTTCATA TTGTCAAAGG GCCAATGGGC
TTTGGTTTTA CTATCGCAGA CAGTCCTGGT GGGGGTGGCC AAAGAGTGAA ACAGATTGTT
GACAGCCCAA GGTGCCGAGG CCTGAAAGAA GGGGATCTCA TAGTGGAAGT TAATAAGAAG
AACGTGCAGG CCCTAACTCA CAACCAAGTG GTGGATATGC TGGTTGAGTG TCCTAAGGGA
AGTGAGGTCA CATTGTTGGT GCAACGAGGA GGGCTGCCAG TTCCCAAGAA GAGCCCAAAG
TCGCAACCAC TGGAAAGGAA AGACAGCCAG AATAGTTCTC AGCACAGTGT TTCCAGCCAC
CGAAGCCTGC ACACAGCATC CCCAAGCCAC AGCACACAGG TGCTCCCCGA GTTCCCACCT
GCAGAGGCCC AAGCTCCAGA TCAAACTGAC AGCTCTGGCC AGAAAAAACC AGATCCTTTT
AAAATCTGGG CCCAGTCCAG GAGCATGTAT GAAAACCGAC CTATGTCACC TTCACCTGCG
TCGGGATTGA GCAAGGGTGA GAGAGAGAGA GAAATCAATT CCACGAATTT TGGAGAATGT
CCGATTCCAG ATTACCAGGA ACAGGACATC TTCCTCTGGA GAAAAGAGAC TGGATTTGGA
TTTAGGATTC TGGGTGGAAA TGAACCAGGG GAACCTATTT ATATTGGTCA CATCGTACCA
CTGGGTGCTG CTGATACTGA CGGCCGCCTG AGGTCTGGAG ATGAATTAAT CTGTGTGGAT
GGGACGCCAG TAATTGGAAA ATCACACCAG CTTGTGGTCC AGCTTATGCA ACAAGCTGCC
AAGCAAGGCC ACGTCAATCT CACGGTGCGG CGTAAAGTGG TTTTTGCGGT GCCCAAAACC
GAGAACGAGG TGCCCTCGCC AGCCTCCTCT CATCACAGTA GCAACCAGCC GGCCTCGCTG
ACAGAAGAGA AGCGCACTCC GCAGGGCAGC CAGAACTCGC TGAACACGGT GAGCTCGGGC
AGCGGCAGCA CCAGCGGCAT CGGCAGTGGC GGCGGCGGGG GCAGCGGCGT GGTCAGCACC
GTGGTGCAGC CCTACGACGT GGAGATCCGG CGCGGGGAGA ACGAGGGCTT CGGCTTCGTC
ATCGTGTCCT CGGTGAGCAG GCCCGAAGCA GGCACGACCT TTGGCAATGC ATGTGTGGCT
ATGCCTCACA AAATAGGTCG GATTATTGAG GGGAGCCCTG CTGACCGCTG TGGCAAGCTG
AAAGTAGGAG ACCGGATCTT GGCAGTAAAT GGATGTTCCA TCACCAACAA ATCCCATTCA
GACATTGTGA ACCTAATCAA GGAAGCGGGA AACACAGTTA CCCTCCGCAT CATTCCTGGG
GATGAGTCCT CGAATGCCAC CTTGCTGACC AATGCAGAGA AGATTGCCAC CATCACCACC
ACACACACCC CTTCTCAGCA AGGGACCCAG GAGACAAGGA ATACCACCAA ACCAAAGCAG
GAATCTCAAT TTGAGTTCAA AGCACCCCAA GCAACACAGG AGCAAGATTT TTACACTGTG
GAACTGGAAA GAGGAGCCAA GGGATTTGGC TTTAGCCTTC GAGGAGGCCG AGAGTATAAC
ATGGACCTCT ATGTTCTGCG CTTAGCAGAG GACGGTCCTG CGGAGAGGTG TGGAAAGATG
AGGATTGGTG ATGAAATTTT AGAGATCAAT GGTGAGACCA CCAAAAACAT GAAGCATTCT
CGAGCTATAG AACTGATTAA GAATGGTGGC CGCAGAGTTC GTCTGTTTCT GAAGCGGGGA
GACGGCTCAG TACCAGAATA TGACCCCAGC AGCGACCGCC ACGGCCCCGC CACCGGTCCA
CAAGGTGTTC CGGAAGTGAG GGCCGGGCCC GACCGCCGGC AGCATCCGTC ATTGGAGTCC
AGTTACCCAC CCGATCTTCA CAAATCATCA CCACATGGGG AAAAGCGGGC ACACGCAAGG
GATCCGAAAG GCAGCAGAGA GTATAGCAGA CAACCCAATG AACACCACAC CTGGAATGGG
ACTTCGAGGA AACCCGACAG CGGGGCATGC CGACCCAAGG ACCGGGCGCC GGAGGGACGG
AGGGACGCGC AGGCCGAGCG CGCGGCAGCC GCCAACGGCC CCAAGAGGCG GTCCCCAGAG
AAGCGGAGGG AGGGCACCCG CAGCGCCGAC AACACTTTGG AGAGGAGGGA GAAGCACGAG
AAGAGACGAG ACGTCTCTCC GGAGCGGAGG CGAGAGCGGT CACCCACCCG CAGGAGAGAC
GGCTCCCCCA GCCGGCGGAG ACGGTCTCTG GAGAGACTCC TGGAGCAGAG GAGGTCCCCC
GAGCGCAGGA GAGGGGGCTC GCCCGAGCGC AGGGCCAAGT CCACCGACCG GAGGCGCGCA
CGCTCCCCCG AGCGCAGGAG AGAGCGGTCC CTGGACAAAA GGAACAGAGA GGACAGAGCC
AGCCACCGAG AAAGGGAAGA GGCGAATCTG AAACAGGATG CCGGCAGAAG TTCCAGACAT
CCCCCGGAGC AGAGAAGGCG ACCTTACAAA GAATGTAGCA CCGACCTCAG TATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265625.1
CDS529..4965
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1407(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1435..1524(+)
Misc Feature(6)1477..1512(+)
Misc Feature(7)1576..1668(+)
Misc Feature(8)1618..1653(+)
Misc Feature(9)1918..2121(+)
Misc Feature(10)1933..2112(+)
Misc Feature(11)2413..2652(+)
Misc Feature(12)2446..2613(+)
Misc Feature(13)3031..3258(+)
Misc Feature(14)3040..3219(+)
Misc Feature(15)3478..3762(+)
Misc Feature(16)3514..3729(+)
Misc Feature(17)3940..4173(+)
Misc Feature(18)3970..4149(+)
Translation

Target ORF information:

RefSeq Version XM_005265568
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265568

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCGGTTCTA
GAAGCCAAAC GGAAGAAGCA GCTTGAGCAG CAGCAGCAGC AGCAACAGCA GCAGCAACAG
CAGCAGCAGC AGCAGCAGCA GCAGCAGACA GAAGAATGGA CAGAAGATCA CTCAGCCCTT
GTGCCTCCTG TTATTCCAAA CCACCCTCCA AGCAATCCAG AGCCAGCCAG AGAAGTTCCA
CTTCAGGGCA AACCCTTTTT TACACGAAAC CCTTCTGAGT TGAAAGGCAA GTTCATTCAC
ACAAAGCTGC GGAAAAGCAG TCGTGGCTTT GGCTTCACGG TGGTTGGAGG GGATGAACCT
GATGAGTTTC TCCAGATCAA GAGCTTGGTC CTAGATGGTC CTGCTGCATT GGATGGCAAG
ATGGAAACAG GGGATGTGAT TGTAAGTGTG AATGACACCT GTGTTTTGGG ACACACACAT
GCTCAAGTTG TGAAGATCTT CCAGTCCATC CCCATTGGTG CCAGCGTGGA CCTTGAACTC
TGCCGAGGTT ATCCATTGCC TTTTGATCCA GATGACCCCA ATACAAGTTT AGTGACCTCG
GTAGCCATTT TGGATAAAGA ACCAATTATT GTGAATGGGC AAGAGACCTA TGATTCACCA
GCTAGCCACA GTAGTAAAAC AGGCAAAGTC AATGGCATGA AGGATGCCAG GCCAAGCAGC
CCAGCGGACG TGGCTTCAAA TAGTTCTCAT GGTTATCCTA ATGACACTGT TTCTTTGGCT
TCCTCCATAG CCACTCAGCC AGAACTCATA ACTGTTCATA TTGTCAAAGG GCCAATGGGC
TTTGGTTTTA CTATCGCAGA CAGTCCTGGT GGGGGTGGCC AAAGAGTGAA ACAGATTGTT
GACAGCCCAA GGTGCCGAGG CCTGAAAGAA GGGGATCTCA TAGTGGAAGT TAATAAGAAG
AACGTGCAGG CCCTAACTCA CAACCAAGTG GTGGATATGC TGGTTGAGTG TCCTAAGGGA
AGTGAGGTCA CATTGTTGGT GCAACGAGGA GGGCTGCCAG TTCCCAAGAA GAGCCCAAAG
TCGCAACCAC TGGAAAGGAA AGACAGCCAG AATAGTTCTC AGCACAGTGT TTCCAGCCAC
CGAAGCCTGC ACACAGCATC CCCAAGCCAC AGCACACAGG TGCTCCCCGA GTTCCCACCT
GCAGAGGCCC AAGCTCCAGA TCAAACTGAC AGCTCTGGCC AGAAAAAACC AGATCCTTTT
AAAATCTGGG CCCAGTCCAG GAGCATGTAT GAAAACCGAC CTATGTCACC TTCACCTGCG
TCGGGATTGA GCAAGGGTGA GAGAGAGAGA GAAATCAATT CCACGAATTT TGGAGAATGT
CCGATTCCAG ATTACCAGGA ACAGGACATC TTCCTCTGGA GAAAAGAGAC TGGATTTGGA
TTTAGGATTC TGGGTGGAAA TGAACCAGGG GAACCTATTT ATATTGGTCA CATCGTACCA
CTGGGTGCTG CTGATACTGA CGGCCGCCTG AGGTCTGGAG ATGAATTAAT CTGTGTGGAT
GGGACGCCAG TAATTGGAAA ATCACACCAG CTTGTGGTCC AGCTTATGCA ACAAGCTGCC
AAGCAAGGCC ACGTCAATCT CACGGTGCGG CGTAAAGTGG TTTTTGCGGT GCCCAAAACC
GAGAACGAGG TGCCCTCGCC AGCCTCCTCT CATCACAGTA GCAACCAGCC GGCCTCGCTG
ACAGAAGAGA AGCGCACTCC GCAGGGCAGC CAGAACTCGC TGAACACGGT GAGCTCGGGC
AGCGGCAGCA CCAGCGGCAT CGGCAGTGGC GGCGGCGGGG GCAGCGGCGT GGTCAGCACC
GTGGTGCAGC CCTACGACGT GGAGATCCGG CGCGGGGAGA ACGAGGGCTT CGGCTTCGTC
ATCGTGTCCT CGGTGAGCAG GCCCGAAGCA GGCACGACCT TTGGCAATGC ATGTGTGGCT
ATGCCTCACA AAATAGGTCG GATTATTGAG GGGAGCCCTG CTGACCGCTG TGGCAAGCTG
AAAGTAGGAG ACCGGATCTT GGCAGTAAAT GGATGTTCCA TCACCAACAA ATCCCATTCA
GACATTGTGA ACCTAATCAA GGAAGCGGGA AACACAGTTA CCCTCCGCAT CATTCCTGGG
GATGAGTCCT CGAATGCCAC CTTGCTGACC AATGCAGAGA AGATTGCCAC CATCACCACC
ACACACACCC CTTCTCAGCA AGGGACCCAG GAGACAAGGA ATACCACCAA ACCAAAGCAG
GAATCTCAAT TTGAGTTCAA AGCACCCCAA GCAACACAGG AGCAAGATTT TTACACTGTG
GAACTGGAAA GAGGAGCCAA GGGATTTGGC TTTAGCCTTC GAGGAGGCCG AGAGTATAAC
ATGGACCTCT ATGTTCTGCG CTTAGCAGAG GACGGTCCTG CGGAGAGGTG TGGAAAGATG
AGGATTGGTG ATGAAATTTT AGAGATCAAT GGTGAGACCA CCAAAAACAT GAAGCATTCT
CGAGCTATAG AACTGATTAA GAATGGTGGC CGCAGAGTTC GTCTGTTTCT GAAGCGGGGA
GACGGCTCAG TACCAGAATA TGACCCCAGC AGCGACCGCC ACGGCCCCGC CACCGGTCCA
CAAGGTGTTC CGGAAGTGAG GGCCGGGCCC GACCGCCGGC AGCATCCGTC ATTGGAGTCC
AGTTACCCAC CCGATCTTCA CAAATCATCA CCACATGGGG AAAAGCGGGC ACACGCAAGG
GATCCGAAAG GCAGCAGAGA GTATAGCAGA CAACCCAATG AACACCACAC CTGGAATGGG
ACTTCGAGGA AACCCGACAG CGGGGCATGC CGACCCAAGG ACCGGGCGCC GGAGGGACGG
AGGGACGCGC AGGCCGAGCG CGCGGCAGCC GCCAACGGCC CCAAGAGGCG GTCCCCAGAG
AAGCGGAGGG AGGGCACCCG CAGCGCCGAC AACACTTTGG AGAGGAGGGA GAAGCACGAG
AAGAGACGAG ACGTCTCTCC GGAGCGGAGG CGAGAGCGGT CACCCACCCG CAGGAGAGAC
GGCTCCCCCA GCCGGCGGAG ACGGTCTCTG GAGAGACTCC TGGAGCAGAG GAGGTCCCCC
GAGCGCAGGA GAGGGGGCTC GCCCGAGCGC AGGGCCAAGT CCACCGACCG GAGGCGCGCA
CGCTCCCCCG AGCGCAGGAG AGAGCGGTCC CTGGACAAAA GGAACAGAGA GGACAGAGCC
AGCCACCGAG AAAGGGAAGA GGCGAATCTG AAACAGGATG CCGGCAGAAG TTCCAGACAT
CCCCCGGAGC AGAGAAGGCG ACCTTACAAA GAATGTAGCA CCGACCTCAG TATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62632
Clone ID Related Accession (Same CDS sequence) XM_011534237
Accession Version XM_011534237.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X11
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCAGTGC CTATGGCTCT ACCCAGGGTG ATGCTGCTGA TCTGGTCTGA TTCCCAAGCT 
GGAGGTAATG GCGTGTGTCC TGCCCTAGTG CTGCTCCCTG ATGGGGAGCA TGCTCAGTTT
CCTGCATTCA GGTACGAGCA GAGACTTGGC AGGATCCGCC CCGTTCAGAG TTGCAGGAAG
AGCCCTGGAT GCCTGTCGCA AATTGGAATA AGTGGGAGGG AGCCACAGGC TGGTACAGGA
GGAAGGCTGA ACAAGGACCT GCGACATTTT CTCAATCAGC GATTCCAGAA GGGGTCTCCT
GATCATGAGC TCCAGCAGAC CATAAGGGAT AACCTTTACC GCCATGCTGT GCCTTGCACA
ACCCGATCTC CCAGAGAAGG AGAAGTGCCT GGCGTGGACT ATAACTTTCT GACTGTGAAG
GAGTTCTTGG ACCTCGAGCA GAGTGGGACT CTTCTGGAAG TCGGCACCTA TGAAGGAAAC
TATTATGGGA CACCCAAGCC TCCTAGCCAG CCAGTCAGTG GGAAAGTGAT CACGACGGAT
GCCTTGCACA GCCTTCAGTC TGGCTCTAAG CAGTCGACCC CGAAGCGAAC CAAGTCCTAC
AATGATATGC AAAATGCTGG CATAGTCCAC GCGGAGAATG AGGAGGAGGA TGACGTTCCT
GAAATGAACA GCAGCTTTAC AGCAGCCGAT TCTGGTGAAC AAGAGGAGCA CACTCTCCAA
GAAACAGCAT TACCACCTGT GAATAGTAGC ATCATCGCTG CTCCCATCAC GGACCCTTCT
CAGAAGTTCC CTCAATACCT ACCTCTTTCT GCAGAGGATA ATTTAGGTCC TCTACCTGAA
AACTGGGAGA TGGCCTATAC TGAAAATGGA GAAGTCTATT TTATAGACCA TAACACGAAA
ACAACATCTT GGTTAGACCC TCGGTGCCTA AACAAGCAGC AGAAGCCACT GGAAGAGTGT
GAAGATGATG AAGAAGGGGT ACACACCGAG GAGCTGGACA GTGAACTAGA ACTGCCTGCT
GGTTGGGAAA AGATTGAAGA CCCTGTCTAT GGTATCTACT ATGTAGACCA CATCAACAGG
AAGACACAAT ATGAGAACCC GGTTCTAGAA GCCAAACGGA AGAAGCAGCT TGAGCAGCAG
CAGCAGCAGC AACAGCAGCA GCAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAGACAGAA
GAATGGACAG AAGATCACTC AGCCCTTGTG CCTCCTGTTA TTCCAAACCA CCCTCCAAGC
AATCCAGAGC CAGCCAGAGA AGTTCCACTT CAGGGCAAAC CCTTTTTTAC ACGAAACCCT
TCTGAGTTGA AAGGCAAGTT CATTCACACA AAGCTGCGGA AAAGCAGTCG TGGCTTTGGC
TTCACGGTGG TTGGAGGGGA TGAACCTGAT GAGTTTCTCC AGATCAAGAG CTTGGTCCTA
GATGGTCCTG CTGCATTGGA TGGCAAGATG GAAACAGGGG ATGTGATTGT AAGTGTGAAT
GACACCTGTG TTTTGGGACA CACACATGCT CAAGTTGTGA AGATCTTCCA GTCCATCCCC
ATTGGTGCCA GCGTGGACCT TGAACTCTGC CGAGGTTATC CATTGCCTTT TGATCCAGAT
GACCCCAATA CAAGTTTAGT GACCTCGGTA GCCATTTTGG ATAAAGAACC AATTATTGTG
AATGGGCAAG AGACCTATGA TTCACCAGCT AGCCACAGTA GTAAAACAGG CAAAGTCAAT
GGCATGAAGG ATGCCAGGCC AAGCAGCCCA GCGGACGTGG CTTCAAATAG TTCTCATGGT
TATCCTAATG ACACTGTTTC TTTGGCTTCC TCCATAGCCA CTCAGCCAGA ACTCATAACT
GTTCATATTG TCAAAGGGCC AATGGGCTTT GGTTTTACTA TCGCAGACAG TCCTGGTGGG
GGTGGCCAAA GAGTGAAACA GATTGTTGAC AGCCCAAGGT GCCGAGGCCT GAAAGAAGGG
GATCTCATAG TGGAAGTTAA TAAGAAGAAC GTGCAGGCCC TAACTCACAA CCAAGTGGTG
GATATGCTGG TTGAGTGTCC TAAGGGAAGT GAGGTCACAT TGTTGGTGCA ACGAGGAGGG
CTGCCAGTTC CCAAGAAGAG CCCAAAGTCG CAACCACTGG AAAGGAAAGA CAGCCAGAAT
AGTTCTCAGC ACAGTGTTTC CAGCCACCGA AGCCTGCACA CAGCATCCCC AAGCCACAGC
ACACAGGTGC TCCCCGAGTT CCCACCTGCA GAGGCCCAAG CTCCAGATCA AACTGACAGC
TCTGGCCAGA AAAAACCAGA TCCTTTTAAA ATCTGGGCCC AGTCCAGGAG CATGTATGAA
AACCGACCTA TGTCACCTTC ACCTGCGTCG GGATTGAGCA AGGGTGAGAG AGAGAGAGAA
ATCAATTCCA CGAATTTTGG AGAATGTCCG ATTCCAGATT ACCAGGAACA GGACATCTTC
CTCTGGAGAA AAGAGACTGG ATTTGGATTT AGGATTCTGG GTGGAAATGA ACCAGGGGAA
CCTATTTATA TTGGTCACAT CGTACCACTG GGTGCTGCTG ATACTGACGG CCGCCTGAGG
TCTGGAGATG AATTAATCTG TGTGGATGGG ACGCCAGTAA TTGGAAAATC ACACCAGCTT
GTGGTCCAGC TTATGCAACA AGCTGCCAAG CAAGGCCACG TCAATCTCAC GGTGCGGCGT
AAAGTGGTTT TTGCGGTGCC CAAAACCGAG AACGAGGTGC CCTCGCCAGC CTCCTCTCAT
CACAGTAGCA ACCAGCCGGC CTCGCTGACA GAAGAGAAGC GCACTCCGCA GGGCAGCCAG
AACTCGCTGA ACACGGTGAG CTCGGGCAGC GGCAGCACCA GCGGCATCGG CAGTGGCGGC
GGCGGGGGCA GCGGCGTGGT CAGCACCGTG GTGCAGCCCT ACGACGTGGA GATCCGGCGC
GGGGAGAACG AGGGCTTCGG CTTCGTCATC GTGTCCTCGG TGAGCAGGCC CGAAGCAGGC
ACGACCTTTG CAGGCAATGC ATGTGTGGCT ATGCCTCACA AAATAGGTCG GATTATTGAG
GGGAGCCCTG CTGACCGCTG TGGCAAGCTG AAAGTAGGAG ACCGGATCTT GGCAGTAAAT
GGATGTTCCA TCACCAACAA ATCCCATTCA GACATTGTGA ACCTAATCAA GGAAGCGGGA
AACACAGTTA CCCTCCGCAT CATTCCTGGG GATGAGTCCT CGAATGCCAC CTTGCTGACC
AATGCAGAGA AGATTGCCAC CATCACCACC ACACACACCC CTTCTCAGCA AGGGACCCAG
GAGACAAGGA ATACCACCAA ACCAAAGCAG GAATCTCAAT TTGAGTTCAA AGCACCCCAA
GCAACACAGG AGCAAGATTT TTACACTGTG GAACTGGAAA GAGGAGCCAA GGGATTTGGC
TTTAGCCTTC GAGGAGGCCG AGAGTATAAC ATGGACCTCT ATGTTCTGCG CTTAGCAGAG
GACGGTCCTG CGGAGAGGTG TGGAAAGATG AGGATTGGTG ATGAAATTTT AGAGATCAAT
GGTGAGACCA CCAAAAACAT GAAGCATTCT CGAGCTATAG AACTGATTAA GAATGGTGGC
CGCAGAGTTC GTCTGTTTCT GAAGCGGGGA GACGGCTCAG TACCAGAATA TGACCCCAGC
AGCGACCGCC ACGGCCCCGC CACCGGTCCA CAAGGTGTTC CGGAAGTGAG GGCCGGGCCC
GACCGCCGGC AGCATCCGTC ATTGGAGTCC AGTTACCCAC CCGATCTTCA CAAATCATCA
CCACATGGGG AAAAGCGGGC ACACGCAAGG GATCCGAAAG GCAGCAGAGA GTATAGCAGA
CAACCCAATG AACACCACAC CTGGAATGGG ACTTCGAGGA AACCCGACAG CGGGGCATGC
CGACCCAAGG ACCGGGCGCC GGAGGGACGG AGGGACGCGC AGGCCGAGCG CGCGGCAGCC
GCCAACGGCC CCAAGAGGCG GTCCCCAGAG AAGCGGAGGG AGGGCACCCG CAGCGCCGAC
AACACTTTGG AGAGGAGGGA GAAGCACGAG AAGAGACGAG ACGTCTCTCC GGAGCGGAGG
CGAGAGCGGT CACCCACCCG CAGGAGAGAC GGCTCCCCCA GCCGGCGGAG ACGGTCTCTG
GAGAGACTCC TGGAGCAGAG GAGGTCCCCC GAGCGCAGGA GAGGGGGCTC GCCCGAGCGC
AGGGCCAAGT CCACCGACCG GAGGCGCGCA CGCTCCCCCG AGCGCAGGAG AGAGCGGTCC
CTGGACAAAA GGAACAGAGA GGACAGAGCC AGCCACCGAG AAAGGGAAGA GGCGAATCTG
AAACAGGATG CCGGCAGAAG TTCCAGACAT CCCCCGGAGC AGAGAAGGCG ACCTTACAAA
GAATGTAGCA CCGACCTCAG TATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532539.1
CDS89..4495
Misc Feature(1)377..895(+)
Misc Feature(2)377..385(+)
Misc Feature(3)923..1012(+)
Misc Feature(4)965..1000(+)
Misc Feature(5)1103..1195(+)
Misc Feature(6)1145..1180(+)
Misc Feature(7)1445..1648(+)
Misc Feature(8)1460..1639(+)
Misc Feature(9)1940..2179(+)
Misc Feature(10)1973..2140(+)
Misc Feature(11)2558..2785(+)
Misc Feature(12)2567..2746(+)
Misc Feature(13)3005..3292(+)
Misc Feature(14)3041..3259(+)
Misc Feature(15)3470..3703(+)
Misc Feature(16)3500..3679(+)
Translation

Target ORF information:

RefSeq Version XM_011534237
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534237

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCAGTGC CTATGGCTCT ACCCAGGGTG ATGCTGCTGA TCTGGTCTGA TTCCCAAGCT 
GGAGGTAATG GCGTGTGTCC TGCCCTAGTG CTGCTCCCTG ATGGGGAGCA TGCTCAGTTT
CCTGCATTCA GGTACGAGCA GAGACTTGGC AGGATCCGCC CCGTTCAGAG TTGCAGGAAG
AGCCCTGGAT GCCTGTCGCA AATTGGAATA AGTGGGAGGG AGCCACAGGC TGGTACAGGA
GGAAGGCTGA ACAAGGACCT GCGACATTTT CTCAATCAGC GATTCCAGAA GGGGTCTCCT
GATCATGAGC TCCAGCAGAC CATAAGGGAT AACCTTTACC GCCATGCTGT GCCTTGCACA
ACCCGATCTC CCAGAGAAGG AGAAGTGCCT GGCGTGGACT ATAACTTTCT GACTGTGAAG
GAGTTCTTGG ACCTCGAGCA GAGTGGGACT CTTCTGGAAG TCGGCACCTA TGAAGGAAAC
TATTATGGGA CACCCAAGCC TCCTAGCCAG CCAGTCAGTG GGAAAGTGAT CACGACGGAT
GCCTTGCACA GCCTTCAGTC TGGCTCTAAG CAGTCGACCC CGAAGCGAAC CAAGTCCTAC
AATGATATGC AAAATGCTGG CATAGTCCAC GCGGAGAATG AGGAGGAGGA TGACGTTCCT
GAAATGAACA GCAGCTTTAC AGCAGCCGAT TCTGGTGAAC AAGAGGAGCA CACTCTCCAA
GAAACAGCAT TACCACCTGT GAATAGTAGC ATCATCGCTG CTCCCATCAC GGACCCTTCT
CAGAAGTTCC CTCAATACCT ACCTCTTTCT GCAGAGGATA ATTTAGGTCC TCTACCTGAA
AACTGGGAGA TGGCCTATAC TGAAAATGGA GAAGTCTATT TTATAGACCA TAACACGAAA
ACAACATCTT GGTTAGACCC TCGGTGCCTA AACAAGCAGC AGAAGCCACT GGAAGAGTGT
GAAGATGATG AAGAAGGGGT ACACACCGAG GAGCTGGACA GTGAACTAGA ACTGCCTGCT
GGTTGGGAAA AGATTGAAGA CCCTGTCTAT GGTATCTACT ATGTAGACCA CATCAACAGG
AAGACACAAT ATGAGAACCC GGTTCTAGAA GCCAAACGGA AGAAGCAGCT TGAGCAGCAG
CAGCAGCAGC AACAGCAGCA GCAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAGACAGAA
GAATGGACAG AAGATCACTC AGCCCTTGTG CCTCCTGTTA TTCCAAACCA CCCTCCAAGC
AATCCAGAGC CAGCCAGAGA AGTTCCACTT CAGGGCAAAC CCTTTTTTAC ACGAAACCCT
TCTGAGTTGA AAGGCAAGTT CATTCACACA AAGCTGCGGA AAAGCAGTCG TGGCTTTGGC
TTCACGGTGG TTGGAGGGGA TGAACCTGAT GAGTTTCTCC AGATCAAGAG CTTGGTCCTA
GATGGTCCTG CTGCATTGGA TGGCAAGATG GAAACAGGGG ATGTGATTGT AAGTGTGAAT
GACACCTGTG TTTTGGGACA CACACATGCT CAAGTTGTGA AGATCTTCCA GTCCATCCCC
ATTGGTGCCA GCGTGGACCT TGAACTCTGC CGAGGTTATC CATTGCCTTT TGATCCAGAT
GACCCCAATA CAAGTTTAGT GACCTCGGTA GCCATTTTGG ATAAAGAACC AATTATTGTG
AATGGGCAAG AGACCTATGA TTCACCAGCT AGCCACAGTA GTAAAACAGG CAAAGTCAAT
GGCATGAAGG ATGCCAGGCC AAGCAGCCCA GCGGACGTGG CTTCAAATAG TTCTCATGGT
TATCCTAATG ACACTGTTTC TTTGGCTTCC TCCATAGCCA CTCAGCCAGA ACTCATAACT
GTTCATATTG TCAAAGGGCC AATGGGCTTT GGTTTTACTA TCGCAGACAG TCCTGGTGGG
GGTGGCCAAA GAGTGAAACA GATTGTTGAC AGCCCAAGGT GCCGAGGCCT GAAAGAAGGG
GATCTCATAG TGGAAGTTAA TAAGAAGAAC GTGCAGGCCC TAACTCACAA CCAAGTGGTG
GATATGCTGG TTGAGTGTCC TAAGGGAAGT GAGGTCACAT TGTTGGTGCA ACGAGGAGGG
CTGCCAGTTC CCAAGAAGAG CCCAAAGTCG CAACCACTGG AAAGGAAAGA CAGCCAGAAT
AGTTCTCAGC ACAGTGTTTC CAGCCACCGA AGCCTGCACA CAGCATCCCC AAGCCACAGC
ACACAGGTGC TCCCCGAGTT CCCACCTGCA GAGGCCCAAG CTCCAGATCA AACTGACAGC
TCTGGCCAGA AAAAACCAGA TCCTTTTAAA ATCTGGGCCC AGTCCAGGAG CATGTATGAA
AACCGACCTA TGTCACCTTC ACCTGCGTCG GGATTGAGCA AGGGTGAGAG AGAGAGAGAA
ATCAATTCCA CGAATTTTGG AGAATGTCCG ATTCCAGATT ACCAGGAACA GGACATCTTC
CTCTGGAGAA AAGAGACTGG ATTTGGATTT AGGATTCTGG GTGGAAATGA ACCAGGGGAA
CCTATTTATA TTGGTCACAT CGTACCACTG GGTGCTGCTG ATACTGACGG CCGCCTGAGG
TCTGGAGATG AATTAATCTG TGTGGATGGG ACGCCAGTAA TTGGAAAATC ACACCAGCTT
GTGGTCCAGC TTATGCAACA AGCTGCCAAG CAAGGCCACG TCAATCTCAC GGTGCGGCGT
AAAGTGGTTT TTGCGGTGCC CAAAACCGAG AACGAGGTGC CCTCGCCAGC CTCCTCTCAT
CACAGTAGCA ACCAGCCGGC CTCGCTGACA GAAGAGAAGC GCACTCCGCA GGGCAGCCAG
AACTCGCTGA ACACGGTGAG CTCGGGCAGC GGCAGCACCA GCGGCATCGG CAGTGGCGGC
GGCGGGGGCA GCGGCGTGGT CAGCACCGTG GTGCAGCCCT ACGACGTGGA GATCCGGCGC
GGGGAGAACG AGGGCTTCGG CTTCGTCATC GTGTCCTCGG TGAGCAGGCC CGAAGCAGGC
ACGACCTTTG CAGGCAATGC ATGTGTGGCT ATGCCTCACA AAATAGGTCG GATTATTGAG
GGGAGCCCTG CTGACCGCTG TGGCAAGCTG AAAGTAGGAG ACCGGATCTT GGCAGTAAAT
GGATGTTCCA TCACCAACAA ATCCCATTCA GACATTGTGA ACCTAATCAA GGAAGCGGGA
AACACAGTTA CCCTCCGCAT CATTCCTGGG GATGAGTCCT CGAATGCCAC CTTGCTGACC
AATGCAGAGA AGATTGCCAC CATCACCACC ACACACACCC CTTCTCAGCA AGGGACCCAG
GAGACAAGGA ATACCACCAA ACCAAAGCAG GAATCTCAAT TTGAGTTCAA AGCACCCCAA
GCAACACAGG AGCAAGATTT TTACACTGTG GAACTGGAAA GAGGAGCCAA GGGATTTGGC
TTTAGCCTTC GAGGAGGCCG AGAGTATAAC ATGGACCTCT ATGTTCTGCG CTTAGCAGAG
GACGGTCCTG CGGAGAGGTG TGGAAAGATG AGGATTGGTG ATGAAATTTT AGAGATCAAT
GGTGAGACCA CCAAAAACAT GAAGCATTCT CGAGCTATAG AACTGATTAA GAATGGTGGC
CGCAGAGTTC GTCTGTTTCT GAAGCGGGGA GACGGCTCAG TACCAGAATA TGACCCCAGC
AGCGACCGCC ACGGCCCCGC CACCGGTCCA CAAGGTGTTC CGGAAGTGAG GGCCGGGCCC
GACCGCCGGC AGCATCCGTC ATTGGAGTCC AGTTACCCAC CCGATCTTCA CAAATCATCA
CCACATGGGG AAAAGCGGGC ACACGCAAGG GATCCGAAAG GCAGCAGAGA GTATAGCAGA
CAACCCAATG AACACCACAC CTGGAATGGG ACTTCGAGGA AACCCGACAG CGGGGCATGC
CGACCCAAGG ACCGGGCGCC GGAGGGACGG AGGGACGCGC AGGCCGAGCG CGCGGCAGCC
GCCAACGGCC CCAAGAGGCG GTCCCCAGAG AAGCGGAGGG AGGGCACCCG CAGCGCCGAC
AACACTTTGG AGAGGAGGGA GAAGCACGAG AAGAGACGAG ACGTCTCTCC GGAGCGGAGG
CGAGAGCGGT CACCCACCCG CAGGAGAGAC GGCTCCCCCA GCCGGCGGAG ACGGTCTCTG
GAGAGACTCC TGGAGCAGAG GAGGTCCCCC GAGCGCAGGA GAGGGGGCTC GCCCGAGCGC
AGGGCCAAGT CCACCGACCG GAGGCGCGCA CGCTCCCCCG AGCGCAGGAG AGAGCGGTCC
CTGGACAAAA GGAACAGAGA GGACAGAGCC AGCCACCGAG AAAGGGAAGA GGCGAATCTG
AAACAGGATG CCGGCAGAAG TTCCAGACAT CCCCCGGAGC AGAGAAGGCG ACCTTACAAA
GAATGTAGCA CCGACCTCAG TATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37592
Clone ID Related Accession (Same CDS sequence) XM_006713411
Accession Version XM_006713411.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACTTCCAGAT TACCAGGAAC AGGACATCTT CCTCTGGAGA
AAAGAGACTG GATTTGGATT TAGGATTCTG GGTGGAAATG AACCAGGGGA ACCTATTTAT
ATTGGTCACA TCGTACCACT GGGTGCTGCT GATACTGACG GCCGCCTGAG GTCTGGAGAT
GAATTAATCT GTGTGGATGG GACGCCAGTA ATTGGAAAAT CACACCAGCT TGTGGTCCAG
CTTATGCAAC AAGCTGCCAA GCAAGGCCAC GTCAATCTCA CGGTGCGGCG TAAAGTGGTT
TTTGCGGTGC CCAAAACCGA GAACGAGGTG CCCTCGCCAG CCTCCTCTCA TCACAGTAGC
AACCAGCCGG CCTCGCTGAC AGAAGAGAAG CGCACTCCGC AGGGCAGCCA GAACTCGCTG
AACACGGTGA GCTCGGGCAG CGGCAGCACC AGCGGCATCG GCAGTGGCGG CGGCGGGGGC
AGCGGCGTGG TCAGCACCGT GGTGCAGCCC TACGACGTGG AGATCCGGCG CGGGGAGAAC
GAGGGCTTCG GCTTCGTCAT CGTGTCCTCG GTGAGCAGGC CCGAAGCAGG CACGACCTTT
GCAGGCAATG CATGTGTGGC TATGCCTCAC AAAATAGGTC GGATTATTGA GGGGAGCCCT
GCTGACCGCT GTGGCAAGCT GAAAGTAGGA GACCGGATCT TGGCAGTAAA TGGATGTTCC
ATCACCAACA AATCCCATTC AGACATTGTG AACCTAATCA AGGAAGCGGG AAACACAGTT
ACCCTCCGCA TCATTCCTGG GGATGAGTCC TCGAATGCCA CCTTGCTGAC CAATGCAGAG
AAGATTGCCA CCATCACCAC CACACACACC CCTTCTCAGC AAGGGACCCA GGAGACAAGG
AATACCACCA AACCAAAGCA GGAATCTCAA TTTGAGTTCA AAGCACCCCA AGCAACACAG
GAGCAAGATT TTTACACTGT GGAACTGGAA AGAGGAGCCA AGGGATTTGG CTTTAGCCTT
CGAGGAGGCC GAGAGTATAA CATGGACCTC TATGTTCTGC GCTTAGCAGA GGACGGTCCT
GCGGAGAGGT GTGGAAAGAT GAGGATTGGT GATGAAATTT TAGAGATCAA TGGTGAGACC
ACCAAAAACA TGAAGCATTC TCGAGCTATA GAACTGATTA AGAATGGTGG CCGCAGAGTT
CGTCTGTTTC TGAAGCGGGG AGACGGCTCA GTACCAGAAT ATGACCCCAG CAGCGACCGC
CACGGCCCCG CCACCGGTCC ACAAGGTGTT CCGGAAGTGA GGGCCGGGCC CGACCGCCGG
CAGCATCCGT CATTGGAGTC CAGTTACCCA CCCGATCTTC ACAAATCATC ACCACATGGG
GAAAAGCGGG CACACGCAAG GGATCCGAAA GGCAGCAGAG AGTATAGCAG ACAACCCAAT
GAACACCACA CCTGGAATGG GACTTCGAGG AAACCCGACA GCGGGGCATG CCGACCCAAG
GACCGGGCGC CGGAGGGACG GAGGGACGCG CAGGCCGAGC GCGCGGCAGC CGCCAACGGC
CCCAAGAGGC GGTCCCCAGA GAAGCGGAGG GAGGGCACCC GCAGCGCCGA CAACACTTTG
GAGAGGAGGG AGAAGCACGA GAAGAGACGA GACGTCTCTC CGGAGCGGAG GCGAGAGCGG
TCACCCACCC GCAGGAGAGA CGGCTCCCCC AGCCGGCGGA GACGGTCTCT GGAGAGACTC
CTGGAGCAGA GGAGGTCCCC CGAGCGCAGG AGAGGGGGCT CGCCCGAGCG CAGGGCCAAG
TCCACCGACC GGAGGCGCGC ACGCTCCCCC GAGCGCAGGA GAGAGCGGTC CCTGGACAAA
AGGAACAGAG AGGACAGAGC CAGCCACCGA GAAAGGGAAG AGGCGAATCT GAAACAGGAT
GCCGGCAGAA GTTCCAGACA TCCCCCGGAG CAGAGAAGGC GACCTTACAA AGAATGTAGC
ACCGACCTCA GTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713474.1
CDS529..4926
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1410(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1438..1527(+)
Misc Feature(6)1480..1515(+)
Misc Feature(7)1618..1710(+)
Misc Feature(8)1660..1695(+)
Misc Feature(9)1960..2163(+)
Misc Feature(10)1975..2154(+)
Misc Feature(11)2455..2694(+)
Misc Feature(12)2488..2655(+)
Misc Feature(13)2989..3216(+)
Misc Feature(14)2998..3177(+)
Misc Feature(15)3436..3723(+)
Misc Feature(16)3472..3690(+)
Misc Feature(17)3901..4134(+)
Misc Feature(18)3931..4110(+)
Translation

Target ORF information:

RefSeq Version XM_006713411
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006713411

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACTTCCAGAT TACCAGGAAC AGGACATCTT CCTCTGGAGA
AAAGAGACTG GATTTGGATT TAGGATTCTG GGTGGAAATG AACCAGGGGA ACCTATTTAT
ATTGGTCACA TCGTACCACT GGGTGCTGCT GATACTGACG GCCGCCTGAG GTCTGGAGAT
GAATTAATCT GTGTGGATGG GACGCCAGTA ATTGGAAAAT CACACCAGCT TGTGGTCCAG
CTTATGCAAC AAGCTGCCAA GCAAGGCCAC GTCAATCTCA CGGTGCGGCG TAAAGTGGTT
TTTGCGGTGC CCAAAACCGA GAACGAGGTG CCCTCGCCAG CCTCCTCTCA TCACAGTAGC
AACCAGCCGG CCTCGCTGAC AGAAGAGAAG CGCACTCCGC AGGGCAGCCA GAACTCGCTG
AACACGGTGA GCTCGGGCAG CGGCAGCACC AGCGGCATCG GCAGTGGCGG CGGCGGGGGC
AGCGGCGTGG TCAGCACCGT GGTGCAGCCC TACGACGTGG AGATCCGGCG CGGGGAGAAC
GAGGGCTTCG GCTTCGTCAT CGTGTCCTCG GTGAGCAGGC CCGAAGCAGG CACGACCTTT
GCAGGCAATG CATGTGTGGC TATGCCTCAC AAAATAGGTC GGATTATTGA GGGGAGCCCT
GCTGACCGCT GTGGCAAGCT GAAAGTAGGA GACCGGATCT TGGCAGTAAA TGGATGTTCC
ATCACCAACA AATCCCATTC AGACATTGTG AACCTAATCA AGGAAGCGGG AAACACAGTT
ACCCTCCGCA TCATTCCTGG GGATGAGTCC TCGAATGCCA CCTTGCTGAC CAATGCAGAG
AAGATTGCCA CCATCACCAC CACACACACC CCTTCTCAGC AAGGGACCCA GGAGACAAGG
AATACCACCA AACCAAAGCA GGAATCTCAA TTTGAGTTCA AAGCACCCCA AGCAACACAG
GAGCAAGATT TTTACACTGT GGAACTGGAA AGAGGAGCCA AGGGATTTGG CTTTAGCCTT
CGAGGAGGCC GAGAGTATAA CATGGACCTC TATGTTCTGC GCTTAGCAGA GGACGGTCCT
GCGGAGAGGT GTGGAAAGAT GAGGATTGGT GATGAAATTT TAGAGATCAA TGGTGAGACC
ACCAAAAACA TGAAGCATTC TCGAGCTATA GAACTGATTA AGAATGGTGG CCGCAGAGTT
CGTCTGTTTC TGAAGCGGGG AGACGGCTCA GTACCAGAAT ATGACCCCAG CAGCGACCGC
CACGGCCCCG CCACCGGTCC ACAAGGTGTT CCGGAAGTGA GGGCCGGGCC CGACCGCCGG
CAGCATCCGT CATTGGAGTC CAGTTACCCA CCCGATCTTC ACAAATCATC ACCACATGGG
GAAAAGCGGG CACACGCAAG GGATCCGAAA GGCAGCAGAG AGTATAGCAG ACAACCCAAT
GAACACCACA CCTGGAATGG GACTTCGAGG AAACCCGACA GCGGGGCATG CCGACCCAAG
GACCGGGCGC CGGAGGGACG GAGGGACGCG CAGGCCGAGC GCGCGGCAGC CGCCAACGGC
CCCAAGAGGC GGTCCCCAGA GAAGCGGAGG GAGGGCACCC GCAGCGCCGA CAACACTTTG
GAGAGGAGGG AGAAGCACGA GAAGAGACGA GACGTCTCTC CGGAGCGGAG GCGAGAGCGG
TCACCCACCC GCAGGAGAGA CGGCTCCCCC AGCCGGCGGA GACGGTCTCT GGAGAGACTC
CTGGAGCAGA GGAGGTCCCC CGAGCGCAGG AGAGGGGGCT CGCCCGAGCG CAGGGCCAAG
TCCACCGACC GGAGGCGCGC ACGCTCCCCC GAGCGCAGGA GAGAGCGGTC CCTGGACAAA
AGGAACAGAG AGGACAGAGC CAGCCACCGA GAAAGGGAAG AGGCGAATCT GAAACAGGAT
GCCGGCAGAA GTTCCAGACA TCCCCCGGAG CAGAGAAGGC GACCTTACAA AGAATGTAGC
ACCGACCTCA GTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37593
Clone ID Related Accession (Same CDS sequence) XM_006713412
Accession Version XM_006713412.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X13
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACTTCC AGATTACCAG GAACAGGACA TCTTCCTCTG GAGAAAAGAG
ACTGGATTTG GATTTAGGAT TCTGGGTGGA AATGAACCAG GGGAACCTAT TTATATTGGT
CACATCGTAC CACTGGGTGC TGCTGATACT GACGGCCGCC TGAGGTCTGG AGATGAATTA
ATCTGTGTGG ATGGGACGCC AGTAATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
CAACAAGCTG CCAAGCAAGG CCACGTCAAT CTCACGGTGC GGCGTAAAGT GGTTTTTGCG
GTGCCCAAAA CCGAGAACGA GGTGCCCTCG CCAGCCTCCT CTCATCACAG TAGCAACCAG
CCGGCCTCGC TGACAGAAGA GAAGCGCACT CCGCAGGGCA GCCAGAACTC GCTGAACACG
GTGAGCTCGG GCAGCGGCAG CACCAGCGGC ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC
GTGGTCAGCA CCGTGGTGCA GCCCTACGAC GTGGAGATCC GGCGCGGGGA GAACGAGGGC
TTCGGCTTCG TCATCGTGTC CTCGGTGAGC AGGCCCGAAG CAGGCACGAC CTTTGCAGGC
AATGCATGTG TGGCTATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
CGCTGTGGCA AGCTGAAAGT AGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
AACAAATCCC ATTCAGACAT TGTGAACCTA ATCAAGGAAG CGGGAAACAC AGTTACCCTC
CGCATCATTC CTGGGGATGA GTCCTCGAAT GCCACCTTGC TGACCAATGC AGAGAAGATT
GCCACCATCA CCACCACACA CACCCCTTCT CAGCAAGGGA CCCAGGAGAC AAGGAATACC
ACCAAACCAA AGCAGGAATC TCAATTTGAG TTCAAAGCAC CCCAAGCAAC ACAGGAGCAA
GATTTTTACA CTGTGGAACT GGAAAGAGGA GCCAAGGGAT TTGGCTTTAG CCTTCGAGGA
GGCCGAGAGT ATAACATGGA CCTCTATGTT CTGCGCTTAG CAGAGGACGG TCCTGCGGAG
AGGTGTGGAA AGATGAGGAT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
AACATGAAGC ATTCTCGAGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTTCGTCTG
TTTCTGAAGC GGGGAGACGG CTCAGTACCA GAATATGACC CCAGCAGCGA CCGCCACGGC
CCCGCCACCG GTCCACAAGG TGTTCCGGAA GTGAGGGCCG GGCCCGACCG CCGGCAGCAT
CCGTCATTGG AGTCCAGTTA CCCACCCGAT CTTCACAAAT CATCACCACA TGGGGAAAAG
CGGGCACACG CAAGGGATCC GAAAGGCAGC AGAGAGTATA GCAGACAACC CAATGAACAC
CACACCTGGA ATGGGACTTC GAGGAAACCC GACAGCGGGG CATGCCGACC CAAGGACCGG
GCGCCGGAGG GACGGAGGGA CGCGCAGGCC GAGCGCGCGG CAGCCGCCAA CGGCCCCAAG
AGGCGGTCCC CAGAGAAGCG GAGGGAGGGC ACCCGCAGCG CCGACAACAC TTTGGAGAGG
AGGGAGAAGC ACGAGAAGAG ACGAGACGTC TCTCCGGAGC GGAGGCGAGA GCGGTCACCC
ACCCGCAGGA GAGACGGCTC CCCCAGCCGG CGGAGACGGT CTCTGGAGAG ACTCCTGGAG
CAGAGGAGGT CCCCCGAGCG CAGGAGAGGG GGCTCGCCCG AGCGCAGGGC CAAGTCCACC
GACCGGAGGC GCGCACGCTC CCCCGAGCGC AGGAGAGAGC GGTCCCTGGA CAAAAGGAAC
AGAGAGGACA GAGCCAGCCA CCGAGAAAGG GAAGAGGCGA ATCTGAAACA GGATGCCGGC
AGAAGTTCCA GACATCCCCC GGAGCAGAGA AGGCGACCTT ACAAAGAATG TAGCACCGAC
CTCAGTATCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713475.1
CDS529..4920
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1407(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1435..1524(+)
Misc Feature(6)1477..1512(+)
Misc Feature(7)1612..1704(+)
Misc Feature(8)1654..1689(+)
Misc Feature(9)1954..2157(+)
Misc Feature(10)1969..2148(+)
Misc Feature(11)2449..2688(+)
Misc Feature(12)2482..2649(+)
Misc Feature(13)2983..3210(+)
Misc Feature(14)2992..3171(+)
Misc Feature(15)3430..3717(+)
Misc Feature(16)3466..3684(+)
Misc Feature(17)3895..4128(+)
Misc Feature(18)3925..4104(+)
Translation

Target ORF information:

RefSeq Version XM_006713412
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006713412

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACTTCC AGATTACCAG GAACAGGACA TCTTCCTCTG GAGAAAAGAG
ACTGGATTTG GATTTAGGAT TCTGGGTGGA AATGAACCAG GGGAACCTAT TTATATTGGT
CACATCGTAC CACTGGGTGC TGCTGATACT GACGGCCGCC TGAGGTCTGG AGATGAATTA
ATCTGTGTGG ATGGGACGCC AGTAATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
CAACAAGCTG CCAAGCAAGG CCACGTCAAT CTCACGGTGC GGCGTAAAGT GGTTTTTGCG
GTGCCCAAAA CCGAGAACGA GGTGCCCTCG CCAGCCTCCT CTCATCACAG TAGCAACCAG
CCGGCCTCGC TGACAGAAGA GAAGCGCACT CCGCAGGGCA GCCAGAACTC GCTGAACACG
GTGAGCTCGG GCAGCGGCAG CACCAGCGGC ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC
GTGGTCAGCA CCGTGGTGCA GCCCTACGAC GTGGAGATCC GGCGCGGGGA GAACGAGGGC
TTCGGCTTCG TCATCGTGTC CTCGGTGAGC AGGCCCGAAG CAGGCACGAC CTTTGCAGGC
AATGCATGTG TGGCTATGCC TCACAAAATA GGTCGGATTA TTGAGGGGAG CCCTGCTGAC
CGCTGTGGCA AGCTGAAAGT AGGAGACCGG ATCTTGGCAG TAAATGGATG TTCCATCACC
AACAAATCCC ATTCAGACAT TGTGAACCTA ATCAAGGAAG CGGGAAACAC AGTTACCCTC
CGCATCATTC CTGGGGATGA GTCCTCGAAT GCCACCTTGC TGACCAATGC AGAGAAGATT
GCCACCATCA CCACCACACA CACCCCTTCT CAGCAAGGGA CCCAGGAGAC AAGGAATACC
ACCAAACCAA AGCAGGAATC TCAATTTGAG TTCAAAGCAC CCCAAGCAAC ACAGGAGCAA
GATTTTTACA CTGTGGAACT GGAAAGAGGA GCCAAGGGAT TTGGCTTTAG CCTTCGAGGA
GGCCGAGAGT ATAACATGGA CCTCTATGTT CTGCGCTTAG CAGAGGACGG TCCTGCGGAG
AGGTGTGGAA AGATGAGGAT TGGTGATGAA ATTTTAGAGA TCAATGGTGA GACCACCAAA
AACATGAAGC ATTCTCGAGC TATAGAACTG ATTAAGAATG GTGGCCGCAG AGTTCGTCTG
TTTCTGAAGC GGGGAGACGG CTCAGTACCA GAATATGACC CCAGCAGCGA CCGCCACGGC
CCCGCCACCG GTCCACAAGG TGTTCCGGAA GTGAGGGCCG GGCCCGACCG CCGGCAGCAT
CCGTCATTGG AGTCCAGTTA CCCACCCGAT CTTCACAAAT CATCACCACA TGGGGAAAAG
CGGGCACACG CAAGGGATCC GAAAGGCAGC AGAGAGTATA GCAGACAACC CAATGAACAC
CACACCTGGA ATGGGACTTC GAGGAAACCC GACAGCGGGG CATGCCGACC CAAGGACCGG
GCGCCGGAGG GACGGAGGGA CGCGCAGGCC GAGCGCGCGG CAGCCGCCAA CGGCCCCAAG
AGGCGGTCCC CAGAGAAGCG GAGGGAGGGC ACCCGCAGCG CCGACAACAC TTTGGAGAGG
AGGGAGAAGC ACGAGAAGAG ACGAGACGTC TCTCCGGAGC GGAGGCGAGA GCGGTCACCC
ACCCGCAGGA GAGACGGCTC CCCCAGCCGG CGGAGACGGT CTCTGGAGAG ACTCCTGGAG
CAGAGGAGGT CCCCCGAGCG CAGGAGAGGG GGCTCGCCCG AGCGCAGGGC CAAGTCCACC
GACCGGAGGC GCGCACGCTC CCCCGAGCGC AGGAGAGAGC GGTCCCTGGA CAAAAGGAAC
AGAGAGGACA GAGCCAGCCA CCGAGAAAGG GAAGAGGCGA ATCTGAAACA GGATGCCGGC
AGAAGTTCCA GACATCCCCC GGAGCAGAGA AGGCGACCTT ACAAAGAATG TAGCACCGAC
CTCAGTATCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37594
Clone ID Related Accession (Same CDS sequence) XM_005265570
Accession Version XM_005265570.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4278bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X14
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACCTATGTCA CCTTCACCTG CGTCGGGATT GAGCAAGGGT
GAGAGAGAGA GAGAAATCAA TTCCACGAAT TTTGGAGAAT GTCCGATTCC AGATTACCAG
GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACTGGATTTG GATTTAGGAT TCTGGGTGGA
AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACT
GACGGCCGCC TGAGGTCTGG AGATGAATTA ATCTGTGTGG ATGGGACGCC AGTAATTGGA
AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCTG CCAAGCAAGG CCACGTCAAT
CTCACGGTGC GGCGTAAAGT GGTTTTTGCG GTGCCCAAAA CCGAGAACGA GGTGCCCTCG
CCAGCCTCCT CTCATCACAG TAGCAACCAG CCGGCCTCGC TGACAGAAGA GAAGCGCACT
CCGCAGGGCA GCCAGAACTC GCTGAACACG GTGAGCTCGG GCAGCGGCAG CACCAGCGGC
ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC GTGGTCAGCA CCGTGGTGCA GCCCTACGAC
GTGGAGATCC GGCGCGGGGA GAACGAGGGC TTCGGCTTCG TCATCGTGTC CTCGGTGAGC
AGGCCCGAAG CAGGCACGAC CTTTGAGTCC TCGAATGCCA CCTTGCTGAC CAATGCAGAG
AAGATTGCCA CCATCACCAC CACACACACC CCTTCTCAGC AAGGGACCCA GGAGACAAGG
AATACCACCA AACCAAAGCA GGAATCTCAA TTTGAGTTCA AAGCACCCCA AGCAACACAG
GAGCAAGATT TTTACACTGT GGAACTGGAA AGAGGAGCCA AGGGATTTGG CTTTAGCCTT
CGAGGAGGCC GAGAGTATAA CATGGACCTC TATGTTCTGC GCTTAGCAGA GGACGGTCCT
GCGGAGAGGT GTGGAAAGAT GAGGATTGGT GATGAAATTT TAGAGATCAA TGGTGAGACC
ACCAAAAACA TGAAGCATTC TCGAGCTATA GAACTGATTA AGAATGGTGG CCGCAGAGTT
CGTCTGTTTC TGAAGCGGGG AGACGGCTCA GTACCAGAAT ATGACCCCAG CAGCGACCGC
CACGGCCCCG CCACCGGTCC ACAAGGTGTT CCGGAAGTGA GGGCCGGGCC CGACCGCCGG
CAGCATCCGT CATTGGAGTC CAGTTACCCA CCCGATCTTC ACAAATCATC ACCACATGGG
GAAAAGCGGG CACACGCAAG GGATCCGAAA GGCAGCAGAG AGTATAGCAG ACAACCCAAT
GAACACCACA CCTGGAATGG GACTTCGAGG AAACCCGACA GCGGGGCATG CCGACCCAAG
GACCGGGCGC CGGAGGGACG GAGGGACGCG CAGGCCGAGC GCGCGGCAGC CGCCAACGGC
CCCAAGAGGC GGTCCCCAGA GAAGCGGAGG GAGGGCACCC GCAGCGCCGA CAACACTTTG
GAGAGGAGGG AGAAGCACGA GAAGAGACGA GACGTCTCTC CGGAGCGGAG GCGAGAGCGG
TCACCCACCC GCAGGAGAGA CGGCTCCCCC AGCCGGCGGA GACGGTCTCT GGAGAGACTC
CTGGAGCAGA GGAGGTCCCC CGAGCGCAGG AGAGGGGGCT CGCCCGAGCG CAGGGCCAAG
TCCACCGACC GGAGGCGCGC ACGCTCCCCC GAGCGCAGGA GAGAGCGGTC CCTGGACAAA
AGGAACAGAG AGGACAGAGC CAGCCACCGA GAAAGGGAAG AGGCGAATCT GAAACAGGAT
GCCGGCAGAA GTTCCAGACA TCCCCCGGAG CAGAGAAGGC GACCTTACAA AGAATGTAGC
ACCGACCTCA GTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265627.1
CDS529..4806
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1410(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1438..1527(+)
Misc Feature(6)1480..1515(+)
Misc Feature(7)1618..1710(+)
Misc Feature(8)1660..1695(+)
Misc Feature(9)1960..2163(+)
Misc Feature(10)1975..2154(+)
Misc Feature(11)2455..2694(+)
Misc Feature(12)2488..2655(+)
Misc Feature(13)3073..3300(+)
Misc Feature(14)3082..3261(+)
Misc Feature(15)3781..4014(+)
Misc Feature(16)3811..3990(+)
Translation

Target ORF information:

RefSeq Version XM_005265570
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265570

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACCTATGTCA CCTTCACCTG CGTCGGGATT GAGCAAGGGT
GAGAGAGAGA GAGAAATCAA TTCCACGAAT TTTGGAGAAT GTCCGATTCC AGATTACCAG
GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACTGGATTTG GATTTAGGAT TCTGGGTGGA
AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACT
GACGGCCGCC TGAGGTCTGG AGATGAATTA ATCTGTGTGG ATGGGACGCC AGTAATTGGA
AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCTG CCAAGCAAGG CCACGTCAAT
CTCACGGTGC GGCGTAAAGT GGTTTTTGCG GTGCCCAAAA CCGAGAACGA GGTGCCCTCG
CCAGCCTCCT CTCATCACAG TAGCAACCAG CCGGCCTCGC TGACAGAAGA GAAGCGCACT
CCGCAGGGCA GCCAGAACTC GCTGAACACG GTGAGCTCGG GCAGCGGCAG CACCAGCGGC
ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC GTGGTCAGCA CCGTGGTGCA GCCCTACGAC
GTGGAGATCC GGCGCGGGGA GAACGAGGGC TTCGGCTTCG TCATCGTGTC CTCGGTGAGC
AGGCCCGAAG CAGGCACGAC CTTTGAGTCC TCGAATGCCA CCTTGCTGAC CAATGCAGAG
AAGATTGCCA CCATCACCAC CACACACACC CCTTCTCAGC AAGGGACCCA GGAGACAAGG
AATACCACCA AACCAAAGCA GGAATCTCAA TTTGAGTTCA AAGCACCCCA AGCAACACAG
GAGCAAGATT TTTACACTGT GGAACTGGAA AGAGGAGCCA AGGGATTTGG CTTTAGCCTT
CGAGGAGGCC GAGAGTATAA CATGGACCTC TATGTTCTGC GCTTAGCAGA GGACGGTCCT
GCGGAGAGGT GTGGAAAGAT GAGGATTGGT GATGAAATTT TAGAGATCAA TGGTGAGACC
ACCAAAAACA TGAAGCATTC TCGAGCTATA GAACTGATTA AGAATGGTGG CCGCAGAGTT
CGTCTGTTTC TGAAGCGGGG AGACGGCTCA GTACCAGAAT ATGACCCCAG CAGCGACCGC
CACGGCCCCG CCACCGGTCC ACAAGGTGTT CCGGAAGTGA GGGCCGGGCC CGACCGCCGG
CAGCATCCGT CATTGGAGTC CAGTTACCCA CCCGATCTTC ACAAATCATC ACCACATGGG
GAAAAGCGGG CACACGCAAG GGATCCGAAA GGCAGCAGAG AGTATAGCAG ACAACCCAAT
GAACACCACA CCTGGAATGG GACTTCGAGG AAACCCGACA GCGGGGCATG CCGACCCAAG
GACCGGGCGC CGGAGGGACG GAGGGACGCG CAGGCCGAGC GCGCGGCAGC CGCCAACGGC
CCCAAGAGGC GGTCCCCAGA GAAGCGGAGG GAGGGCACCC GCAGCGCCGA CAACACTTTG
GAGAGGAGGG AGAAGCACGA GAAGAGACGA GACGTCTCTC CGGAGCGGAG GCGAGAGCGG
TCACCCACCC GCAGGAGAGA CGGCTCCCCC AGCCGGCGGA GACGGTCTCT GGAGAGACTC
CTGGAGCAGA GGAGGTCCCC CGAGCGCAGG AGAGGGGGCT CGCCCGAGCG CAGGGCCAAG
TCCACCGACC GGAGGCGCGC ACGCTCCCCC GAGCGCAGGA GAGAGCGGTC CCTGGACAAA
AGGAACAGAG AGGACAGAGC CAGCCACCGA GAAAGGGAAG AGGCGAATCT GAAACAGGAT
GCCGGCAGAA GTTCCAGACA TCCCCCGGAG CAGAGAAGGC GACCTTACAA AGAATGTAGC
ACCGACCTCA GTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37595
Clone ID Related Accession (Same CDS sequence) XM_005265571
Accession Version XM_005265571.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X15
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACTTCC AGATTACCAG GAACAGGACA TCTTCCTCTG GAGAAAAGAG
ACTGGATTTG GATTTAGGAT TCTGGGTGGA AATGAACCAG GGGAACCTAT TTATATTGGT
CACATCGTAC CACTGGGTGC TGCTGATACT GACGGCCGCC TGAGGTCTGG AGATGAATTA
ATCTGTGTGG ATGGGACGCC AGTAATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
CAACAAGCTG CCAAGCAAGG CCACGTCAAT CTCACGGTGC GGCGTAAAGT GGTTTTTGCG
GTGCCCAAAA CCGAGAACGA GGTGCCCTCG CCAGCCTCCT CTCATCACAG TAGCAACCAG
CCGGCCTCGC TGACAGAAGA GAAGCGCACT CCGCAGGGCA GCCAGAACTC GCTGAACACG
GTGAGCTCGG GCAGCGGCAG CACCAGCGGC ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC
GTGGTCAGCA CCGTGGTGCA GCCCTACGAC GTGGAGATCC GGCGCGGGGA GAACGAGGGC
TTCGGCTTCG TCATCGTGTC CTCGGTGAGC AGGCCCGAAG CAGGCACGAC CTTTGAGTCC
TCGAATGCCA CCTTGCTGAC CAATGCAGAG AAGATTGCCA CCATCACCAC CACACACACC
CCTTCTCAGC AAGGGACCCA GGAGACAAGG AATACCACCA AACCAAAGCA GGAATCTCAA
TTTGAGTTCA AAGCACCCCA AGCAACACAG GAGCAAGATT TTTACACTGT GGAACTGGAA
AGAGGAGCCA AGGGATTTGG CTTTAGCCTT CGAGGAGGCC GAGAGTATAA CATGGACCTC
TATGTTCTGC GCTTAGCAGA GGACGGTCCT GCGGAGAGGT GTGGAAAGAT GAGGATTGGT
GATGAAATTT TAGAGATCAA TGGTGAGACC ACCAAAAACA TGAAGCATTC TCGAGCTATA
GAACTGATTA AGAATGGTGG CCGCAGAGTT CGTCTGTTTC TGAAGCGGGG AGACGGCTCA
GTACCAGAAT ATGACCCCAG CAGCGACCGC CACGGCCCCG CCACCGGTCC ACAAGGTGTT
CCGGAAGTGA GGGCCGGGCC CGACCGCCGG CAGCATCCGT CATTGGAGTC CAGTTACCCA
CCCGATCTTC ACAAATCATC ACCACATGGG GAAAAGCGGG CACACGCAAG GGATCCGAAA
GGCAGCAGAG AGTATAGCAG ACAACCCAAT GAACACCACA CCTGGAATGG GACTTCGAGG
AAACCCGACA GCGGGGCATG CCGACCCAAG GACCGGGCGC CGGAGGGACG GAGGGACGCG
CAGGCCGAGC GCGCGGCAGC CGCCAACGGC CCCAAGAGGC GGTCCCCAGA GAAGCGGAGG
GAGGGCACCC GCAGCGCCGA CAACACTTTG GAGAGGAGGG AGAAGCACGA GAAGAGACGA
GACGTCTCTC CGGAGCGGAG GCGAGAGCGG TCACCCACCC GCAGGAGAGA CGGCTCCCCC
AGCCGGCGGA GACGGTCTCT GGAGAGACTC CTGGAGCAGA GGAGGTCCCC CGAGCGCAGG
AGAGGGGGCT CGCCCGAGCG CAGGGCCAAG TCCACCGACC GGAGGCGCGC ACGCTCCCCC
GAGCGCAGGA GAGAGCGGTC CCTGGACAAA AGGAACAGAG AGGACAGAGC CAGCCACCGA
GAAAGGGAAG AGGCGAATCT GAAACAGGAT GCCGGCAGAA GTTCCAGACA TCCCCCGGAG
CAGAGAAGGC GACCTTACAA AGAATGTAGC ACCGACCTCA GTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265628.1
CDS529..4716
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1407(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1435..1524(+)
Misc Feature(6)1477..1512(+)
Misc Feature(7)1612..1704(+)
Misc Feature(8)1654..1689(+)
Misc Feature(9)1954..2157(+)
Misc Feature(10)1969..2148(+)
Misc Feature(11)2449..2688(+)
Misc Feature(12)2482..2649(+)
Misc Feature(13)2983..3210(+)
Misc Feature(14)2992..3171(+)
Misc Feature(15)3691..3924(+)
Misc Feature(16)3721..3900(+)
Translation

Target ORF information:

RefSeq Version XM_005265571
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265571

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACTTCC AGATTACCAG GAACAGGACA TCTTCCTCTG GAGAAAAGAG
ACTGGATTTG GATTTAGGAT TCTGGGTGGA AATGAACCAG GGGAACCTAT TTATATTGGT
CACATCGTAC CACTGGGTGC TGCTGATACT GACGGCCGCC TGAGGTCTGG AGATGAATTA
ATCTGTGTGG ATGGGACGCC AGTAATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
CAACAAGCTG CCAAGCAAGG CCACGTCAAT CTCACGGTGC GGCGTAAAGT GGTTTTTGCG
GTGCCCAAAA CCGAGAACGA GGTGCCCTCG CCAGCCTCCT CTCATCACAG TAGCAACCAG
CCGGCCTCGC TGACAGAAGA GAAGCGCACT CCGCAGGGCA GCCAGAACTC GCTGAACACG
GTGAGCTCGG GCAGCGGCAG CACCAGCGGC ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC
GTGGTCAGCA CCGTGGTGCA GCCCTACGAC GTGGAGATCC GGCGCGGGGA GAACGAGGGC
TTCGGCTTCG TCATCGTGTC CTCGGTGAGC AGGCCCGAAG CAGGCACGAC CTTTGAGTCC
TCGAATGCCA CCTTGCTGAC CAATGCAGAG AAGATTGCCA CCATCACCAC CACACACACC
CCTTCTCAGC AAGGGACCCA GGAGACAAGG AATACCACCA AACCAAAGCA GGAATCTCAA
TTTGAGTTCA AAGCACCCCA AGCAACACAG GAGCAAGATT TTTACACTGT GGAACTGGAA
AGAGGAGCCA AGGGATTTGG CTTTAGCCTT CGAGGAGGCC GAGAGTATAA CATGGACCTC
TATGTTCTGC GCTTAGCAGA GGACGGTCCT GCGGAGAGGT GTGGAAAGAT GAGGATTGGT
GATGAAATTT TAGAGATCAA TGGTGAGACC ACCAAAAACA TGAAGCATTC TCGAGCTATA
GAACTGATTA AGAATGGTGG CCGCAGAGTT CGTCTGTTTC TGAAGCGGGG AGACGGCTCA
GTACCAGAAT ATGACCCCAG CAGCGACCGC CACGGCCCCG CCACCGGTCC ACAAGGTGTT
CCGGAAGTGA GGGCCGGGCC CGACCGCCGG CAGCATCCGT CATTGGAGTC CAGTTACCCA
CCCGATCTTC ACAAATCATC ACCACATGGG GAAAAGCGGG CACACGCAAG GGATCCGAAA
GGCAGCAGAG AGTATAGCAG ACAACCCAAT GAACACCACA CCTGGAATGG GACTTCGAGG
AAACCCGACA GCGGGGCATG CCGACCCAAG GACCGGGCGC CGGAGGGACG GAGGGACGCG
CAGGCCGAGC GCGCGGCAGC CGCCAACGGC CCCAAGAGGC GGTCCCCAGA GAAGCGGAGG
GAGGGCACCC GCAGCGCCGA CAACACTTTG GAGAGGAGGG AGAAGCACGA GAAGAGACGA
GACGTCTCTC CGGAGCGGAG GCGAGAGCGG TCACCCACCC GCAGGAGAGA CGGCTCCCCC
AGCCGGCGGA GACGGTCTCT GGAGAGACTC CTGGAGCAGA GGAGGTCCCC CGAGCGCAGG
AGAGGGGGCT CGCCCGAGCG CAGGGCCAAG TCCACCGACC GGAGGCGCGC ACGCTCCCCC
GAGCGCAGGA GAGAGCGGTC CCTGGACAAA AGGAACAGAG AGGACAGAGC CAGCCACCGA
GAAAGGGAAG AGGCGAATCT GAAACAGGAT GCCGGCAGAA GTTCCAGACA TCCCCCGGAG
CAGAGAAGGC GACCTTACAA AGAATGTAGC ACCGACCTCA GTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62633
Clone ID Related Accession (Same CDS sequence) XM_006713413
Accession Version XM_006713413.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X16
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578806759. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGGTGTTCC TACTAAAAGA GAAAACAGGC ACAACCCGAT CTCCCAGAGA AGGAGAAGTG 
CCTGGCGTGG ACTATAACTT TCTGACTGTG AAGGAGTTCT TGGACCTCGA GCAGAGTGGG
ACTCTTCTGG AAGTCGGCAC CTATGAAGGA AACTATTATG GGACACCCAA GCCTCCTAGC
CAGCCAGTCA GTGGGAAAGT GATCACGACG GATGCCTTGC ACAGCCTTCA GTCTGGCTCT
AAGCAGTCGA CCCCGAAGCG AACCAAGTCC TACAATGATA TGCAAAATGC TGGCATAGTC
CACGCGGAGA ATGAGGAGGA GGATGACGTT CCTGAAATGA ACAGCAGCTT TACAGCAGCC
GATTCTGGTG AACAAGAGGA GCACACTCTC CAAGAAACAG CATTACCACC TGTGAATAGT
AGCATCATCG CTGCTCCCAT CACGGACCCT TCTCAGAAGT TCCCTCAATA CCTACCTCTT
TCTGCAGAGG ATAATTTAGG TCCTCTACCT GAAAACTGGG AGATGGCCTA TACTGAAAAT
GGAGAAGTCT ATTTTATAGA CCATAACACG AAAACAACAT CTTGGTTAGA CCCTCGGTGC
CTAAACAAGC AGCAGAAGCC ACTGGAAGAG TGTGAAGATG ATGAAGAAGG GGTACACACC
GAGGAGCTGG ACAGTGAACT AGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCGGTTCTA
GAAGCCAAAC GGAAGAAGCA GCTTGAGCAG CAGCAGCAGC AGCAACAGCA GCAGCAACAG
CAGCAGCAGC AGCAGCAGCA GCAGCAGACA GAAGAATGGA CAGAAGATCA CTCAGCCCTT
GTGCCTCCTG TTATTCCAAA CCACCCTCCA AGCAATCCAG AGCCAGCCAG AGAAGTTCCA
CTTCAGGGCA AACCCTTTTT TACACGAAAC CCTTCTGAGT TGAAAGGCAA GTTCATTCAC
ACAAAGCTGC GGAAAAGCAG TCGTGGCTTT GGCTTCACGG TGGTTGGAGG GGATGAACCT
GATGAGTTTC TCCAGATCAA GAGCTTGGTC CTAGATGGTC CTGCTGCATT GGATGGCAAG
ATGGAAACAG GGGATGTGAT TGTAAGTGTG AATGACACCT GTGTTTTGGG ACACACACAT
GCTCAAGTTG TGAAGATCTT CCAGTCCATC CCCATTGGTG CCAGCGTGGA CCTTGAACTC
TGCCGAGGTT ATCCATTGCC TTTTGATCCA GATGACCCCA ATACAAGTTT AGTGACCTCG
GTAGCCATTT TGGATAAAGA ACCAATTATT GTGAATGGGC AAGAGACCTA TGATTCACCA
GCTAGCCACA GTAGTAAAAC AGGCAAAGTC AATGGCATGA AGGATGCCAG GCCAAGCAGC
CCAGCGGACG TGGCTTCAAA TAGTTCTCAT GGTTATCCTA ATGACACTGT TTCTTTGGCT
TCCTCCATAG CCACTCAGCC AGAACTCATA ACTGTTCATA TTGTCAAAGG GCCAATGGGC
TTTGGTTTTA CTATCGCAGA CAGTCCTGGT GGGGGTGGCC AAAGAGTGAA ACAGATTGTT
GACAGCCCAA GGTGCCGAGG CCTGAAAGAA GGGGATCTCA TAGTGGAAGT TAATAAGAAG
AACGTGCAGG CCCTAACTCA CAACCAAGTG GTGGATATGC TGGTTGAGTG TCCTAAGGGA
AGTGAGGTCA CATTGTTGGT GCAACGAGGA GGGCTGCCAG TTCCCAAGAA GAGCCCAAAG
TCGCAACCAC TGGAAAGGAA AGACAGCCAG AATAGTTCTC AGCACAGTGT TTCCAGCCAC
CGAAGCCTGC ACACAGCATC CCCAAGCCAC AGCACACAGG TGCTCCCCGA GTTCCCACCT
GCAGAGGCCC AAGCTCCAGA TCAAACTGAC AGCTCTGGCC AGAAAAAACC AGATCCTTTT
AAAATCTGGG CCCAGTCCAG GAGCATGTAT GAAAACCGAC CTATGTCACC TTCACCTGCG
TCGGGATTGA GCAAGGGTGA GAGAGAGAGA GAAATCAATT CCACGAATTT TGGAGAATGT
CCGATTCCAG ATTACCAGGA ACAGGACATC TTCCTCTGGA GAAAAGAGAC TGGATTTGGA
TTTAGGATTC TGGGTGGAAA TGAACCAGGG GAACCTATTT ATATTGGTCA CATCGTACCA
CTGGGTGCTG CTGATACTGA CGGCCGCCTG AGGTCTGGAG ATGAATTAAT CTGTGTGGAT
GGGACGCCAG TAATTGGAAA ATCACACCAG CTTGTGGTCC AGCTTATGCA ACAAGCTGCC
AAGCAAGGCC ACGTCAATCT CACGGTGCGG CGTAAAGTGG TTTTTGCGGT GCCCAAAACC
GAGAACGAGG TGCCCTCGCC AGCCTCCTCT CATCACAGTA GCAACCAGCC GGCCTCGCTG
ACAGAAGAGA AGCGCACTCC GCAGGGCAGC CAGAACTCGC TGAACACGGT GAGCTCGGGC
AGCGGCAGCA CCAGCGGCAT CGGCAGTGGC GGCGGCGGGG GCAGCGGCGT GGTCAGCACC
GTGGTGCAGC CCTACGACGT GGAGATCCGG CGCGGGGAGA ACGAGGGCTT CGGCTTCGTC
ATCGTGTCCT CGGTGAGCAG GCCCGAAGCA GGCACGACCT TTGCAGGCAA TGCATGTGTG
GCTATGCCTC ACAAAATAGG TCGGATTATT GAGGGGAGCC CTGCTGACCG CTGTGGCAAG
CTGAAAGTAG GAGACCGGAT CTTGGCAGTA AATGGATGTT CCATCACCAA CAAATCCCAT
TCAGACATTG TGAACCTAAT CAAGGAAGCG GGAAACACAG TTACCCTCCG CATCATTCCT
GGGGATGAGT CCTCGAATGC CACCTTGCTG ACCAATGCAG AGAAGATTGC CACCATCACC
ACCACACACA CCCCTTCTCA GCAAGGGACC CAGGAGACAA GGAATACCAC CAAACCAAAG
CAGGAATCTC AATTTGAGTT CAAAGCACCC CAAGCAACAC AGGAGCAAGA TTTTTACACT
GTGGAACTGG AAAGAGGAGC CAAGGGATTT GGCTTTAGCC TTCGAGGAGG CCGAGAGTAT
AACATGGACC TCTATGTTCT GCGCTTAGCA GAGGACGGTC CTGCGGAGAG GTGTGGAAAG
ATGAGGATTG GTGATGAAAT TTTAGAGATC AATGGTGAGA CCACCAAAAA CATGAAGCAT
TCTCGAGCTA TAGAACTGAT TAAGAATGGT GGCCGCAGAG TTCGTCTGTT TCTGAAGCGG
GGAGACGGCT CAGTACCAGA ATATGACCCC AGCAGCGACC GCCACGGCCC CGCCACCGGT
CCACAAGGTG TTCCGGAAGT GAGGGCCGGG CCCGACCGCC GGCAGCATCC GTCATTGGAG
TCCAGTTACC CACCCGATCT TCACAAATCA TCACCACATG GGGAAAAGCG GGCACACGCA
AGGGATCCGA AAGGCAGCAG AGAGTATAGC AGACAACCCA ATGAACACCA CACCTGGAAT
GGGACTTCGA GGAAACCCGA CAGCGGGGCA TGCCGACCCA AGGACCGGGC GCCGGAGGGA
CGGAGGGACG CGCAGGCCGA GCGCGCGGCA GCCGCCAACG GCCCCAAGAG GCGGTCCCCA
GAGAAGCGGA GGGAGGGCAC CCGCAGCGCC GACAACACTT TGGAGAGGAG GGAGAAGCAC
GAGAAGAGAC GAGACGTCTC TCCGGAGCGG AGGCGAGAGC GGTCACCCAC CCGCAGGAGA
GACGGCTCCC CCAGCCGGCG GAGACGGTCT CTGGAGAGAC TCCTGGAGCA GAGGAGGTCC
CCCGAGCGCA GGAGAGGGGG CTCGCCCGAG CGCAGGGCCA AGTCCACCGA CCGGAGGCGC
GCACGCTCCC CCGAGCGCAG GAGAGAGCGG TCCCTGGACA AAAGGAACAG AGAGGACAGA
GCCAGCCACC GAGAAAGGGA AGAGGCGAAT CTGAAACAGG ATGCCGGCAG AAGTTCCAGA
CATCCCCCGG AGCAGAGAAG GCGACCTTAC AAAGAATGTA GCACCGACCT CAGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713476.2
CDS16..4095
Misc Feature(1)46..495(+)
Misc Feature(2)523..612(+)
Misc Feature(3)565..600(+)
Misc Feature(4)703..795(+)
Misc Feature(5)745..780(+)
Misc Feature(6)1045..1248(+)
Misc Feature(7)1060..1239(+)
Misc Feature(8)1540..1779(+)
Misc Feature(9)1573..1740(+)
Misc Feature(10)2158..2385(+)
Misc Feature(11)2167..2346(+)
Misc Feature(12)2605..2892(+)
Misc Feature(13)2641..2859(+)
Misc Feature(14)3070..3303(+)
Misc Feature(15)3100..3279(+)
Translation

Target ORF information:

RefSeq Version XM_006713413
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X16, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006713413

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGGTGTTCC TACTAAAAGA GAAAACAGGC ACAACCCGAT CTCCCAGAGA AGGAGAAGTG 
CCTGGCGTGG ACTATAACTT TCTGACTGTG AAGGAGTTCT TGGACCTCGA GCAGAGTGGG
ACTCTTCTGG AAGTCGGCAC CTATGAAGGA AACTATTATG GGACACCCAA GCCTCCTAGC
CAGCCAGTCA GTGGGAAAGT GATCACGACG GATGCCTTGC ACAGCCTTCA GTCTGGCTCT
AAGCAGTCGA CCCCGAAGCG AACCAAGTCC TACAATGATA TGCAAAATGC TGGCATAGTC
CACGCGGAGA ATGAGGAGGA GGATGACGTT CCTGAAATGA ACAGCAGCTT TACAGCAGCC
GATTCTGGTG AACAAGAGGA GCACACTCTC CAAGAAACAG CATTACCACC TGTGAATAGT
AGCATCATCG CTGCTCCCAT CACGGACCCT TCTCAGAAGT TCCCTCAATA CCTACCTCTT
TCTGCAGAGG ATAATTTAGG TCCTCTACCT GAAAACTGGG AGATGGCCTA TACTGAAAAT
GGAGAAGTCT ATTTTATAGA CCATAACACG AAAACAACAT CTTGGTTAGA CCCTCGGTGC
CTAAACAAGC AGCAGAAGCC ACTGGAAGAG TGTGAAGATG ATGAAGAAGG GGTACACACC
GAGGAGCTGG ACAGTGAACT AGAACTGCCT GCTGGTTGGG AAAAGATTGA AGACCCTGTC
TATGGTATCT ACTATGTAGA CCACATCAAC AGGAAGACAC AATATGAGAA CCCGGTTCTA
GAAGCCAAAC GGAAGAAGCA GCTTGAGCAG CAGCAGCAGC AGCAACAGCA GCAGCAACAG
CAGCAGCAGC AGCAGCAGCA GCAGCAGACA GAAGAATGGA CAGAAGATCA CTCAGCCCTT
GTGCCTCCTG TTATTCCAAA CCACCCTCCA AGCAATCCAG AGCCAGCCAG AGAAGTTCCA
CTTCAGGGCA AACCCTTTTT TACACGAAAC CCTTCTGAGT TGAAAGGCAA GTTCATTCAC
ACAAAGCTGC GGAAAAGCAG TCGTGGCTTT GGCTTCACGG TGGTTGGAGG GGATGAACCT
GATGAGTTTC TCCAGATCAA GAGCTTGGTC CTAGATGGTC CTGCTGCATT GGATGGCAAG
ATGGAAACAG GGGATGTGAT TGTAAGTGTG AATGACACCT GTGTTTTGGG ACACACACAT
GCTCAAGTTG TGAAGATCTT CCAGTCCATC CCCATTGGTG CCAGCGTGGA CCTTGAACTC
TGCCGAGGTT ATCCATTGCC TTTTGATCCA GATGACCCCA ATACAAGTTT AGTGACCTCG
GTAGCCATTT TGGATAAAGA ACCAATTATT GTGAATGGGC AAGAGACCTA TGATTCACCA
GCTAGCCACA GTAGTAAAAC AGGCAAAGTC AATGGCATGA AGGATGCCAG GCCAAGCAGC
CCAGCGGACG TGGCTTCAAA TAGTTCTCAT GGTTATCCTA ATGACACTGT TTCTTTGGCT
TCCTCCATAG CCACTCAGCC AGAACTCATA ACTGTTCATA TTGTCAAAGG GCCAATGGGC
TTTGGTTTTA CTATCGCAGA CAGTCCTGGT GGGGGTGGCC AAAGAGTGAA ACAGATTGTT
GACAGCCCAA GGTGCCGAGG CCTGAAAGAA GGGGATCTCA TAGTGGAAGT TAATAAGAAG
AACGTGCAGG CCCTAACTCA CAACCAAGTG GTGGATATGC TGGTTGAGTG TCCTAAGGGA
AGTGAGGTCA CATTGTTGGT GCAACGAGGA GGGCTGCCAG TTCCCAAGAA GAGCCCAAAG
TCGCAACCAC TGGAAAGGAA AGACAGCCAG AATAGTTCTC AGCACAGTGT TTCCAGCCAC
CGAAGCCTGC ACACAGCATC CCCAAGCCAC AGCACACAGG TGCTCCCCGA GTTCCCACCT
GCAGAGGCCC AAGCTCCAGA TCAAACTGAC AGCTCTGGCC AGAAAAAACC AGATCCTTTT
AAAATCTGGG CCCAGTCCAG GAGCATGTAT GAAAACCGAC CTATGTCACC TTCACCTGCG
TCGGGATTGA GCAAGGGTGA GAGAGAGAGA GAAATCAATT CCACGAATTT TGGAGAATGT
CCGATTCCAG ATTACCAGGA ACAGGACATC TTCCTCTGGA GAAAAGAGAC TGGATTTGGA
TTTAGGATTC TGGGTGGAAA TGAACCAGGG GAACCTATTT ATATTGGTCA CATCGTACCA
CTGGGTGCTG CTGATACTGA CGGCCGCCTG AGGTCTGGAG ATGAATTAAT CTGTGTGGAT
GGGACGCCAG TAATTGGAAA ATCACACCAG CTTGTGGTCC AGCTTATGCA ACAAGCTGCC
AAGCAAGGCC ACGTCAATCT CACGGTGCGG CGTAAAGTGG TTTTTGCGGT GCCCAAAACC
GAGAACGAGG TGCCCTCGCC AGCCTCCTCT CATCACAGTA GCAACCAGCC GGCCTCGCTG
ACAGAAGAGA AGCGCACTCC GCAGGGCAGC CAGAACTCGC TGAACACGGT GAGCTCGGGC
AGCGGCAGCA CCAGCGGCAT CGGCAGTGGC GGCGGCGGGG GCAGCGGCGT GGTCAGCACC
GTGGTGCAGC CCTACGACGT GGAGATCCGG CGCGGGGAGA ACGAGGGCTT CGGCTTCGTC
ATCGTGTCCT CGGTGAGCAG GCCCGAAGCA GGCACGACCT TTGCAGGCAA TGCATGTGTG
GCTATGCCTC ACAAAATAGG TCGGATTATT GAGGGGAGCC CTGCTGACCG CTGTGGCAAG
CTGAAAGTAG GAGACCGGAT CTTGGCAGTA AATGGATGTT CCATCACCAA CAAATCCCAT
TCAGACATTG TGAACCTAAT CAAGGAAGCG GGAAACACAG TTACCCTCCG CATCATTCCT
GGGGATGAGT CCTCGAATGC CACCTTGCTG ACCAATGCAG AGAAGATTGC CACCATCACC
ACCACACACA CCCCTTCTCA GCAAGGGACC CAGGAGACAA GGAATACCAC CAAACCAAAG
CAGGAATCTC AATTTGAGTT CAAAGCACCC CAAGCAACAC AGGAGCAAGA TTTTTACACT
GTGGAACTGG AAAGAGGAGC CAAGGGATTT GGCTTTAGCC TTCGAGGAGG CCGAGAGTAT
AACATGGACC TCTATGTTCT GCGCTTAGCA GAGGACGGTC CTGCGGAGAG GTGTGGAAAG
ATGAGGATTG GTGATGAAAT TTTAGAGATC AATGGTGAGA CCACCAAAAA CATGAAGCAT
TCTCGAGCTA TAGAACTGAT TAAGAATGGT GGCCGCAGAG TTCGTCTGTT TCTGAAGCGG
GGAGACGGCT CAGTACCAGA ATATGACCCC AGCAGCGACC GCCACGGCCC CGCCACCGGT
CCACAAGGTG TTCCGGAAGT GAGGGCCGGG CCCGACCGCC GGCAGCATCC GTCATTGGAG
TCCAGTTACC CACCCGATCT TCACAAATCA TCACCACATG GGGAAAAGCG GGCACACGCA
AGGGATCCGA AAGGCAGCAG AGAGTATAGC AGACAACCCA ATGAACACCA CACCTGGAAT
GGGACTTCGA GGAAACCCGA CAGCGGGGCA TGCCGACCCA AGGACCGGGC GCCGGAGGGA
CGGAGGGACG CGCAGGCCGA GCGCGCGGCA GCCGCCAACG GCCCCAAGAG GCGGTCCCCA
GAGAAGCGGA GGGAGGGCAC CCGCAGCGCC GACAACACTT TGGAGAGGAG GGAGAAGCAC
GAGAAGAGAC GAGACGTCTC TCCGGAGCGG AGGCGAGAGC GGTCACCCAC CCGCAGGAGA
GACGGCTCCC CCAGCCGGCG GAGACGGTCT CTGGAGAGAC TCCTGGAGCA GAGGAGGTCC
CCCGAGCGCA GGAGAGGGGG CTCGCCCGAG CGCAGGGCCA AGTCCACCGA CCGGAGGCGC
GCACGCTCCC CCGAGCGCAG GAGAGAGCGG TCCCTGGACA AAAGGAACAG AGAGGACAGA
GCCAGCCACC GAGAAAGGGA AGAGGCGAAT CTGAAACAGG ATGCCGGCAG AAGTTCCAGA
CATCCCCCGG AGCAGAGAAG GCGACCTTAC AAAGAATGTA GCACCGACCT CAGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37597
Clone ID Related Accession (Same CDS sequence) XM_006713414
Accession Version XM_006713414.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X17
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578806761. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACCTATGTCA CCTTCACCTG CGTCGGGATT GAGCAAGGGT
GAGAGAGAGA GAGAAATCAA TTCCACGAAT TTTGGAGAAT GTCCGATTCC AGATTACCAG
GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACTGGATTTG GATTTAGGAT TCTGGGTGGA
AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACT
GACGGCCGCC TGAGGTCTGG AGATGAATTA ATCTGTGTGG ATGGGACGCC AGTAATTGGA
AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCTG CCAAGCAAGG CCACGTCAAT
CTCACGGTGC GGCGTAAAGT GGTTTTTGCG GTGCCCAAAA CCGAGAACGA GGTGCCCTCG
CCAGCCTCCT CTCATCACAG TAGCAACCAG CCGGCCTCGC TGACAGAAGA GAAGCGCACT
CCGCAGGGCA GCCAGAACTC GCTGAACACG GTGAGCTCGG GCAGCGGCAG CACCAGCGGC
ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC GTGGTCAGCA CCGTGGTGCA GCCCTACGAC
GTGGAGATCC GGCGCGGGGA GAACGAGGGC TTCGGCTTCG TCATCGTGTC CTCGGTGAGC
AGGCCCGAAG CAGGCACGAC CTTTGCAGGC AATGCATGTG TGGCTATGCC TCACAAAATA
GGTCGGATTA TTGAGGGGAG CCCTGCTGAC CGCTGTGGCA AGCTGAAAGT AGGAGACCGG
ATCTTGGCAG TAAATGGATG TTCCATCACC AACAAATCCC ATTCAGACAT TGTGAACCTA
ATCAAGGAAG CGGGAAACAC AGTTACCCTC CGCATCATTC CTGGGGATGA GTCCTCGAAT
GCCACCTTGC TGACCAATGC AGAGAAGATT GCCACCATCA CCACCACACA CACCCCTTCT
CAGCAAGGGA CCCAGGAGAC AAGGAATACC ACCAAACCAA AGCAGGAATC TCAATTTGAG
TTCAAAGCAC CCCAAGCAAC ACAGGAGCAA GATTTTTACA CTGTGGAACT GGAAAGAGGA
GCCAAGGGAT TTGGCTTTAG CCTTCGAGGA GGCCGAGAGT ATAACATGGA CCTCTATGTT
CTGCGCTTAG CAGAGGACGG TCCTGCGGAG AGGTGTGGAA AGATGAGGAT TGGTGATGAA
ATTTTAGAGA TCAATGGTGA GACCACCAAA AACATGAAGC ATTCTCGAGC TATAGAACTG
ATTAAGAATG GTGGCCGCAG AGTTCGTCTG TTTCTGAAGC GGGGAGACGG CTCAGTACCA
GAATATGCGA TGATACCTCC TAATATCGCT GCATGTATGA GAAATGAAAA GCTCGGGGAG
GCTTGCTTCT ACCTTATGGG CCATAATCAA ACTACGACCC CAGCAGCGAC CGCCACGGCC
CCGCCACCGG TCCACAAGGT GTTCCGGAAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713477.1
CDS529..4401
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1410(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1438..1527(+)
Misc Feature(6)1480..1515(+)
Misc Feature(7)1618..1710(+)
Misc Feature(8)1660..1695(+)
Misc Feature(9)1960..2163(+)
Misc Feature(10)1975..2154(+)
Misc Feature(11)2455..2694(+)
Misc Feature(12)2488..2655(+)
Misc Feature(13)3073..3300(+)
Misc Feature(14)3082..3261(+)
Misc Feature(15)3520..3807(+)
Misc Feature(16)3556..3774(+)
Misc Feature(17)3985..4218(+)
Misc Feature(18)4015..4194(+)
Translation

Target ORF information:

RefSeq Version XM_006713414
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X17, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006713414

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACCTATGTCA CCTTCACCTG CGTCGGGATT GAGCAAGGGT
GAGAGAGAGA GAGAAATCAA TTCCACGAAT TTTGGAGAAT GTCCGATTCC AGATTACCAG
GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACTGGATTTG GATTTAGGAT TCTGGGTGGA
AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACT
GACGGCCGCC TGAGGTCTGG AGATGAATTA ATCTGTGTGG ATGGGACGCC AGTAATTGGA
AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCTG CCAAGCAAGG CCACGTCAAT
CTCACGGTGC GGCGTAAAGT GGTTTTTGCG GTGCCCAAAA CCGAGAACGA GGTGCCCTCG
CCAGCCTCCT CTCATCACAG TAGCAACCAG CCGGCCTCGC TGACAGAAGA GAAGCGCACT
CCGCAGGGCA GCCAGAACTC GCTGAACACG GTGAGCTCGG GCAGCGGCAG CACCAGCGGC
ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC GTGGTCAGCA CCGTGGTGCA GCCCTACGAC
GTGGAGATCC GGCGCGGGGA GAACGAGGGC TTCGGCTTCG TCATCGTGTC CTCGGTGAGC
AGGCCCGAAG CAGGCACGAC CTTTGCAGGC AATGCATGTG TGGCTATGCC TCACAAAATA
GGTCGGATTA TTGAGGGGAG CCCTGCTGAC CGCTGTGGCA AGCTGAAAGT AGGAGACCGG
ATCTTGGCAG TAAATGGATG TTCCATCACC AACAAATCCC ATTCAGACAT TGTGAACCTA
ATCAAGGAAG CGGGAAACAC AGTTACCCTC CGCATCATTC CTGGGGATGA GTCCTCGAAT
GCCACCTTGC TGACCAATGC AGAGAAGATT GCCACCATCA CCACCACACA CACCCCTTCT
CAGCAAGGGA CCCAGGAGAC AAGGAATACC ACCAAACCAA AGCAGGAATC TCAATTTGAG
TTCAAAGCAC CCCAAGCAAC ACAGGAGCAA GATTTTTACA CTGTGGAACT GGAAAGAGGA
GCCAAGGGAT TTGGCTTTAG CCTTCGAGGA GGCCGAGAGT ATAACATGGA CCTCTATGTT
CTGCGCTTAG CAGAGGACGG TCCTGCGGAG AGGTGTGGAA AGATGAGGAT TGGTGATGAA
ATTTTAGAGA TCAATGGTGA GACCACCAAA AACATGAAGC ATTCTCGAGC TATAGAACTG
ATTAAGAATG GTGGCCGCAG AGTTCGTCTG TTTCTGAAGC GGGGAGACGG CTCAGTACCA
GAATATGCGA TGATACCTCC TAATATCGCT GCATGTATGA GAAATGAAAA GCTCGGGGAG
GCTTGCTTCT ACCTTATGGG CCATAATCAA ACTACGACCC CAGCAGCGAC CGCCACGGCC
CCGCCACCGG TCCACAAGGT GTTCCGGAAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37598
Clone ID Related Accession (Same CDS sequence) XM_005265574
Accession Version XM_005265574.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X18
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530373370. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACCTAT GTCACCTTCA CCTGCGTCGG GATTGAGCAA GGGTGAGAGA
GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCCGA TTCCAGATTA CCAGGAACAG
GACATCTTCC TCTGGAGAAA AGAGACTGGA TTTGGATTTA GGATTCTGGG TGGAAATGAA
CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACTGACGGC
CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA CGCCAGTAAT TGGAAAATCA
CACCAGCTTG TGGTCCAGCT TATGCAACAA GCTGCCAAGC AAGGCCACGT CAATCTCACG
GTGCGGCGTA AAGTGGTTTT TGCGGTGCCC AAAACCGAGA ACGAGGTGCC CTCGCCAGCC
TCCTCTCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACAG AAGAGAAGCG CACTCCGCAG
GGCAGCCAGA ACTCGCTGAA CACGGTGAGC TCGGGCAGCG GCAGCACCAG CGGCATCGGC
AGTGGCGGCG GCGGGGGCAG CGGCGTGGTC AGCACCGTGG TGCAGCCCTA CGACGTGGAG
ATCCGGCGCG GGGAGAACGA GGGCTTCGGC TTCGTCATCG TGTCCTCGGT GAGCAGGCCC
GAAGCAGGCA CGACCTTTGC AGGCAATGCA TGTGTGGCTA TGCCTCACAA AATAGGTCGG
ATTATTGAGG GGAGCCCTGC TGACCGCTGT GGCAAGCTGA AAGTAGGAGA CCGGATCTTG
GCAGTAAATG GATGTTCCAT CACCAACAAA TCCCATTCAG ACATTGTGAA CCTAATCAAG
GAAGCGGGAA ACACAGTTAC CCTCCGCATC ATTCCTGGGG ATGAGTCCTC GAATGCCACC
TTGCTGACCA ATGCAGAGAA GATTGCCACC ATCACCACCA CACACACCCC TTCTCAGCAA
GGGACCCAGG AGACAAGGAA TACCACCAAA CCAAAGCAGG AATCTCAATT TGAGTTCAAA
GCACCCCAAG CAACACAGGA GCAAGATTTT TACACTGTGG AACTGGAAAG AGGAGCCAAG
GGATTTGGCT TTAGCCTTCG AGGAGGCCGA GAGTATAACA TGGACCTCTA TGTTCTGCGC
TTAGCAGAGG ACGGTCCTGC GGAGAGGTGT GGAAAGATGA GGATTGGTGA TGAAATTTTA
GAGATCAATG GTGAGACCAC CAAAAACATG AAGCATTCTC GAGCTATAGA ACTGATTAAG
AATGGTGGCC GCAGAGTTCG TCTGTTTCTG AAGCGGGGAG ACGGCTCAGT ACCAGAATAT
GCGATGATAC CTCCTAATAT CGCTGCATGT ATGAGAAATG AAAAGCTCGG GGAGGCTTGC
TTCTACCTTA TGGGCCATAA TCAAACTACG ACCCCAGCAG CGACCGCCAC GGCCCCGCCA
CCGGTCCACA AGGTGTTCCG GAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265631.1
CDS529..4395
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1407(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1435..1524(+)
Misc Feature(6)1477..1512(+)
Misc Feature(7)1612..1704(+)
Misc Feature(8)1654..1689(+)
Misc Feature(9)1954..2157(+)
Misc Feature(10)1969..2148(+)
Misc Feature(11)2449..2688(+)
Misc Feature(12)2482..2649(+)
Misc Feature(13)3067..3294(+)
Misc Feature(14)3076..3255(+)
Misc Feature(15)3514..3801(+)
Misc Feature(16)3550..3768(+)
Misc Feature(17)3979..4212(+)
Misc Feature(18)4009..4188(+)
Translation

Target ORF information:

RefSeq Version XM_005265574
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X18, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265574

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACCTAT GTCACCTTCA CCTGCGTCGG GATTGAGCAA GGGTGAGAGA
GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCCGA TTCCAGATTA CCAGGAACAG
GACATCTTCC TCTGGAGAAA AGAGACTGGA TTTGGATTTA GGATTCTGGG TGGAAATGAA
CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACTGACGGC
CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA CGCCAGTAAT TGGAAAATCA
CACCAGCTTG TGGTCCAGCT TATGCAACAA GCTGCCAAGC AAGGCCACGT CAATCTCACG
GTGCGGCGTA AAGTGGTTTT TGCGGTGCCC AAAACCGAGA ACGAGGTGCC CTCGCCAGCC
TCCTCTCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACAG AAGAGAAGCG CACTCCGCAG
GGCAGCCAGA ACTCGCTGAA CACGGTGAGC TCGGGCAGCG GCAGCACCAG CGGCATCGGC
AGTGGCGGCG GCGGGGGCAG CGGCGTGGTC AGCACCGTGG TGCAGCCCTA CGACGTGGAG
ATCCGGCGCG GGGAGAACGA GGGCTTCGGC TTCGTCATCG TGTCCTCGGT GAGCAGGCCC
GAAGCAGGCA CGACCTTTGC AGGCAATGCA TGTGTGGCTA TGCCTCACAA AATAGGTCGG
ATTATTGAGG GGAGCCCTGC TGACCGCTGT GGCAAGCTGA AAGTAGGAGA CCGGATCTTG
GCAGTAAATG GATGTTCCAT CACCAACAAA TCCCATTCAG ACATTGTGAA CCTAATCAAG
GAAGCGGGAA ACACAGTTAC CCTCCGCATC ATTCCTGGGG ATGAGTCCTC GAATGCCACC
TTGCTGACCA ATGCAGAGAA GATTGCCACC ATCACCACCA CACACACCCC TTCTCAGCAA
GGGACCCAGG AGACAAGGAA TACCACCAAA CCAAAGCAGG AATCTCAATT TGAGTTCAAA
GCACCCCAAG CAACACAGGA GCAAGATTTT TACACTGTGG AACTGGAAAG AGGAGCCAAG
GGATTTGGCT TTAGCCTTCG AGGAGGCCGA GAGTATAACA TGGACCTCTA TGTTCTGCGC
TTAGCAGAGG ACGGTCCTGC GGAGAGGTGT GGAAAGATGA GGATTGGTGA TGAAATTTTA
GAGATCAATG GTGAGACCAC CAAAAACATG AAGCATTCTC GAGCTATAGA ACTGATTAAG
AATGGTGGCC GCAGAGTTCG TCTGTTTCTG AAGCGGGGAG ACGGCTCAGT ACCAGAATAT
GCGATGATAC CTCCTAATAT CGCTGCATGT ATGAGAAATG AAAAGCTCGG GGAGGCTTGC
TTCTACCTTA TGGGCCATAA TCAAACTACG ACCCCAGCAG CGACCGCCAC GGCCCCGCCA
CCGGTCCACA AGGTGTTCCG GAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62634
Clone ID Related Accession (Same CDS sequence) XM_011534238
Accession Version XM_011534238.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X19
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGCAAAATG CTGGCATAGT CCACGCGGAG AATGAGGAGG AGGATGACGT TCCTGAAATG 
AACAGCAGCT TTACAGCCGA TTCTGGTGAA CAAGAGGAGC ACACTCTCCA AGAAACAGCA
TTACCACCTG TGAATAGTAG CATCATCGCT GCTCCCATCA CGGACCCTTC TCAGAAGTTC
CCTCAATACC TACCTCTTTC TGCAGAGGAT AATTTAGGTC CTCTACCTGA AAACTGGGAG
ATGGCCTATA CTGAAAATGG AGAAGTCTAT TTTATAGACC ATAACACGAA AACAACATCT
TGGTTAGACC CTCGGTGCCT AAACAAGCAG CAGAAGCCAC TGGAAGAGTG TGAAGATGAT
GAAGAAGGGG TACACACCGA GGAGCTGGAC AGTGAACTAG AACTGCCTGC TGGTTGGGAA
AAGATTGAAG ACCCTGTCTA TGGTATCTAC TATGTAGACC ACATCAACAG GAAGACACAA
TATGAGAACC CGGTTCTAGA AGCCAAACGG AAGAAGCAGC TTGAGCAGCA GCAGCAGCAG
CAACAGCAGC AGCAACAGCA GCAGCAGCAG CAGCAGCAGC AGCAGACAGA AGAATGGACA
GAAGATCACT CAGCCCTTGT GCCTCCTGTT ATTCCAAACC ACCCTCCAAG CAATCCAGAG
CCAGCCAGAG AAGTTCCACT TCAGGGCAAA CCCTTTTTTA CACGAAACCC TTCTGAGTTG
AAAGGCAAGT TCATTCACAC AAAGCTGCGG AAAAGCAGTC GTGGCTTTGG CTTCACGGTG
GTTGGAGGGG ATGAACCTGA TGAGTTTCTC CAGATCAAGA GCTTGGTCCT AGATGGTCCT
GCTGCATTGG ATGGCAAGAT GGAAACAGGG GATGTGATTG TAAGTGTGAA TGACACCTGT
GTTTTGGGAC ACACACATGC TCAAGTTGTG AAGATCTTCC AGTCCATCCC CATTGGTGCC
AGCGTGGACC TTGAACTCTG CCGAGGTTAT CCATTGCCTT TTGATCCAGA TGACCCCAAT
ACAAGTTTAG TGACCTCGGT AGCCATTTTG GATAAAGAAC CAATTATTGT GAATGGGCAA
GAGACCTATG ATTCACCAGC TAGCCACAGT AGTAAAACAG GCAAAGTCAA TGGCATGAAG
GATGCCAGGC CAAGCAGCCC AGCGGACGTG GCTTCAAATA GTTCTCATGG TTATCCTAAT
GACACTGTTT CTTTGGCTTC CTCCATAGCC ACTCAGCCAG AACTCATAAC TGTTCATATT
GTCAAAGGGC CAATGGGCTT TGGTTTTACT ATCGCAGACA GTCCTGGTGG GGGTGGCCAA
AGAGTGAAAC AGATTGTTGA CAGCCCAAGG TGCCGAGGCC TGAAAGAAGG GGATCTCATA
GTGGAAGTTA ATAAGAAGAA CGTGCAGGCC CTAACTCACA ACCAAGTGGT GGATATGCTG
GTTGAGTGTC CTAAGGGAAG TGAGGTCACA TTGTTGGTGC AACGAGGAGG GCTGCCAGTT
CCCAAGAAGA GCCCAAAGTC GCAACCACTG GAAAGGAAAG ACAGCCAGAA TAGTTCTCAG
CACAGTGTTT CCAGCCACCG AAGCCTGCAC ACAGCATCCC CAAGCCACAG CACACAGGTG
CTCCCCGAGT TCCCACCTGC AGAGGCCCAA GCTCCAGATC AAACTGACAG CTCTGGCCAG
AAAAAACCAG ATCCTTTTAA AATCTGGGCC CAGTCCAGGA GCATGTATGA AAACCGACCT
ATGTCACCTT CACCTGCGTC GGGATTGAGC AAGGGTGAGA GAGAGAGAGA AATCAATTCC
ACGAATTTTG GAGAATGTCC GATTCCAGAT TACCAGGAAC AGGACATCTT CCTCTGGAGA
AAAGAGACTG GATTTGGATT TAGGATTCTG GGTGGAAATG AACCAGGGGA ACCTATTTAT
ATTGGTCACA TCGTACCACT GGGTGCTGCT GATACTGACG GCCGCCTGAG GTCTGGAGAT
GAATTAATCT GTGTGGATGG GACGCCAGTA ATTGGAAAAT CACACCAGCT TGTGGTCCAG
CTTATGCAAC AAGCTGCCAA GCAAGGCCAC GTCAATCTCA CGGTGCGGCG TAAAGTGGTT
TTTGCGGTGC CCAAAACCGA GAACGAGGTG CCCTCGCCAG CCTCCTCTCA TCACAGTAGC
AACCAGCCGG CCTCGCTGAC AGAAGAGAAG CGCACTCCGC AGGGCAGCCA GAACTCGCTG
AACACGGTGA GCTCGGGCAG CGGCAGCACC AGCGGCATCG GCAGTGGCGG CGGCGGGGGC
AGCGGCGTGG TCAGCACCGT GGTGCAGCCC TACGACGTGG AGATCCGGCG CGGGGAGAAC
GAGGGCTTCG GCTTCGTCAT CGTGTCCTCG GTGAGCAGGC CCGAAGCAGG CACGACCTTT
GCAGGCAATG CATGTGTGGC TATGCCTCAC AAAATAGGTC GGATTATTGA GGGGAGCCCT
GCTGACCGCT GTGGCAAGCT GAAAGTAGGA GACCGGATCT TGGCAGTAAA TGGATGTTCC
ATCACCAACA AATCCCATTC AGACATTGTG AACCTAATCA AGGAAGCGGG AAACACAGTT
ACCCTCCGCA TCATTCCTGG GGATGAGTCC TCGAATGCCA CCTTGCTGAC CAATGCAGAG
AAGATTGCCA CCATCACCAC CACACACACC CCTTCTCAGC AAGGGACCCA GGAGACAAGG
AATACCACCA AACCAAAGCA GGAATCTCAA TTTGAGTTCA AAGCACCCCA AGCAACACAG
GAGCAAGATT TTTACACTGT GGAACTGGAA AGAGGAGCCA AGGGATTTGG CTTTAGCCTT
CGAGGAGGCC GAGAGTATAA CATGGACCTC TATGTTCTGC GCTTAGCAGA GGACGGTCCT
GCGGAGAGGT GTGGAAAGAT GAGGATTGGT GATGAAATTT TAGAGATCAA TGGTGAGACC
ACCAAAAACA TGAAGCATTC TCGAGCTATA GAACTGATTA AGAATGGTGG CCGCAGAGTT
CGTCTGTTTC TGAAGCGGGG AGACGGCTCA GTACCAGAAT ATGACCCCAG CAGCGACCGC
CACGGCCCCG CCACCGGTCC ACAAGGTGTT CCGGAAGTGA GGGCCGGGCC CGACCGCCGG
CAGCATCCGT CATTGGAGTC CAGTTACCCA CCCGATCTTC ACAAATCATC ACCACATGGG
GAAAAGCGGG CACACGCAAG GGATCCGAAA GGCAGCAGAG AGTATAGCAG ACAACCCAAT
GAACACCACA CCTGGAATGG GACTTCGAGG AAACCCGACA GCGGGGCATG CCGACCCAAG
GACCGGGCGC CGGAGGGACG GAGGGACGCG CAGGCCGAGC GCGCGGCAGC CGCCAACGGC
CCCAAGAGGC GGTCCCCAGA GAAGCGGAGG GAGGGCACCC GCAGCGCCGA CAACACTTTG
GAGAGGAGGG AGAAGCACGA GAAGAGACGA GACGTCTCTC CGGAGCGGAG GCGAGAGCGG
TCACCCACCC GCAGGAGAGA CGGCTCCCCC AGCCGGCGGA GACGGTCTCT GGAGAGACTC
CTGGAGCAGA GGAGGTCCCC CGAGCGCAGG AGAGGGGGCT CGCCCGAGCG CAGGGCCAAG
TCCACCGACC GGAGGCGCGC ACGCTCCCCC GAGCGCAGGA GAGAGCGGTC CCTGGACAAA
AGGAACAGAG AGGACAGAGC CAGCCACCGA GAAAGGGAAG AGGCGAATCT GAAACAGGAT
GCCGGCAGAA GTTCCAGACA TCCCCCGGAG CAGAGAAGGC GACCTTACAA AGAATGTAGC
ACCGACCTCA GTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532540.1
CDS270..4067
Misc Feature(1)495..584(+)
Misc Feature(2)537..572(+)
Misc Feature(3)675..767(+)
Misc Feature(4)717..752(+)
Misc Feature(5)1017..1220(+)
Misc Feature(6)1032..1211(+)
Misc Feature(7)1512..1751(+)
Misc Feature(8)1545..1712(+)
Misc Feature(9)2130..2357(+)
Misc Feature(10)2139..2318(+)
Misc Feature(11)2577..2864(+)
Misc Feature(12)2613..2831(+)
Misc Feature(13)3042..3275(+)
Misc Feature(14)3072..3251(+)
Translation

Target ORF information:

RefSeq Version XM_011534238
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X19, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534238

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGCAAAATG CTGGCATAGT CCACGCGGAG AATGAGGAGG AGGATGACGT TCCTGAAATG 
AACAGCAGCT TTACAGCCGA TTCTGGTGAA CAAGAGGAGC ACACTCTCCA AGAAACAGCA
TTACCACCTG TGAATAGTAG CATCATCGCT GCTCCCATCA CGGACCCTTC TCAGAAGTTC
CCTCAATACC TACCTCTTTC TGCAGAGGAT AATTTAGGTC CTCTACCTGA AAACTGGGAG
ATGGCCTATA CTGAAAATGG AGAAGTCTAT TTTATAGACC ATAACACGAA AACAACATCT
TGGTTAGACC CTCGGTGCCT AAACAAGCAG CAGAAGCCAC TGGAAGAGTG TGAAGATGAT
GAAGAAGGGG TACACACCGA GGAGCTGGAC AGTGAACTAG AACTGCCTGC TGGTTGGGAA
AAGATTGAAG ACCCTGTCTA TGGTATCTAC TATGTAGACC ACATCAACAG GAAGACACAA
TATGAGAACC CGGTTCTAGA AGCCAAACGG AAGAAGCAGC TTGAGCAGCA GCAGCAGCAG
CAACAGCAGC AGCAACAGCA GCAGCAGCAG CAGCAGCAGC AGCAGACAGA AGAATGGACA
GAAGATCACT CAGCCCTTGT GCCTCCTGTT ATTCCAAACC ACCCTCCAAG CAATCCAGAG
CCAGCCAGAG AAGTTCCACT TCAGGGCAAA CCCTTTTTTA CACGAAACCC TTCTGAGTTG
AAAGGCAAGT TCATTCACAC AAAGCTGCGG AAAAGCAGTC GTGGCTTTGG CTTCACGGTG
GTTGGAGGGG ATGAACCTGA TGAGTTTCTC CAGATCAAGA GCTTGGTCCT AGATGGTCCT
GCTGCATTGG ATGGCAAGAT GGAAACAGGG GATGTGATTG TAAGTGTGAA TGACACCTGT
GTTTTGGGAC ACACACATGC TCAAGTTGTG AAGATCTTCC AGTCCATCCC CATTGGTGCC
AGCGTGGACC TTGAACTCTG CCGAGGTTAT CCATTGCCTT TTGATCCAGA TGACCCCAAT
ACAAGTTTAG TGACCTCGGT AGCCATTTTG GATAAAGAAC CAATTATTGT GAATGGGCAA
GAGACCTATG ATTCACCAGC TAGCCACAGT AGTAAAACAG GCAAAGTCAA TGGCATGAAG
GATGCCAGGC CAAGCAGCCC AGCGGACGTG GCTTCAAATA GTTCTCATGG TTATCCTAAT
GACACTGTTT CTTTGGCTTC CTCCATAGCC ACTCAGCCAG AACTCATAAC TGTTCATATT
GTCAAAGGGC CAATGGGCTT TGGTTTTACT ATCGCAGACA GTCCTGGTGG GGGTGGCCAA
AGAGTGAAAC AGATTGTTGA CAGCCCAAGG TGCCGAGGCC TGAAAGAAGG GGATCTCATA
GTGGAAGTTA ATAAGAAGAA CGTGCAGGCC CTAACTCACA ACCAAGTGGT GGATATGCTG
GTTGAGTGTC CTAAGGGAAG TGAGGTCACA TTGTTGGTGC AACGAGGAGG GCTGCCAGTT
CCCAAGAAGA GCCCAAAGTC GCAACCACTG GAAAGGAAAG ACAGCCAGAA TAGTTCTCAG
CACAGTGTTT CCAGCCACCG AAGCCTGCAC ACAGCATCCC CAAGCCACAG CACACAGGTG
CTCCCCGAGT TCCCACCTGC AGAGGCCCAA GCTCCAGATC AAACTGACAG CTCTGGCCAG
AAAAAACCAG ATCCTTTTAA AATCTGGGCC CAGTCCAGGA GCATGTATGA AAACCGACCT
ATGTCACCTT CACCTGCGTC GGGATTGAGC AAGGGTGAGA GAGAGAGAGA AATCAATTCC
ACGAATTTTG GAGAATGTCC GATTCCAGAT TACCAGGAAC AGGACATCTT CCTCTGGAGA
AAAGAGACTG GATTTGGATT TAGGATTCTG GGTGGAAATG AACCAGGGGA ACCTATTTAT
ATTGGTCACA TCGTACCACT GGGTGCTGCT GATACTGACG GCCGCCTGAG GTCTGGAGAT
GAATTAATCT GTGTGGATGG GACGCCAGTA ATTGGAAAAT CACACCAGCT TGTGGTCCAG
CTTATGCAAC AAGCTGCCAA GCAAGGCCAC GTCAATCTCA CGGTGCGGCG TAAAGTGGTT
TTTGCGGTGC CCAAAACCGA GAACGAGGTG CCCTCGCCAG CCTCCTCTCA TCACAGTAGC
AACCAGCCGG CCTCGCTGAC AGAAGAGAAG CGCACTCCGC AGGGCAGCCA GAACTCGCTG
AACACGGTGA GCTCGGGCAG CGGCAGCACC AGCGGCATCG GCAGTGGCGG CGGCGGGGGC
AGCGGCGTGG TCAGCACCGT GGTGCAGCCC TACGACGTGG AGATCCGGCG CGGGGAGAAC
GAGGGCTTCG GCTTCGTCAT CGTGTCCTCG GTGAGCAGGC CCGAAGCAGG CACGACCTTT
GCAGGCAATG CATGTGTGGC TATGCCTCAC AAAATAGGTC GGATTATTGA GGGGAGCCCT
GCTGACCGCT GTGGCAAGCT GAAAGTAGGA GACCGGATCT TGGCAGTAAA TGGATGTTCC
ATCACCAACA AATCCCATTC AGACATTGTG AACCTAATCA AGGAAGCGGG AAACACAGTT
ACCCTCCGCA TCATTCCTGG GGATGAGTCC TCGAATGCCA CCTTGCTGAC CAATGCAGAG
AAGATTGCCA CCATCACCAC CACACACACC CCTTCTCAGC AAGGGACCCA GGAGACAAGG
AATACCACCA AACCAAAGCA GGAATCTCAA TTTGAGTTCA AAGCACCCCA AGCAACACAG
GAGCAAGATT TTTACACTGT GGAACTGGAA AGAGGAGCCA AGGGATTTGG CTTTAGCCTT
CGAGGAGGCC GAGAGTATAA CATGGACCTC TATGTTCTGC GCTTAGCAGA GGACGGTCCT
GCGGAGAGGT GTGGAAAGAT GAGGATTGGT GATGAAATTT TAGAGATCAA TGGTGAGACC
ACCAAAAACA TGAAGCATTC TCGAGCTATA GAACTGATTA AGAATGGTGG CCGCAGAGTT
CGTCTGTTTC TGAAGCGGGG AGACGGCTCA GTACCAGAAT ATGACCCCAG CAGCGACCGC
CACGGCCCCG CCACCGGTCC ACAAGGTGTT CCGGAAGTGA GGGCCGGGCC CGACCGCCGG
CAGCATCCGT CATTGGAGTC CAGTTACCCA CCCGATCTTC ACAAATCATC ACCACATGGG
GAAAAGCGGG CACACGCAAG GGATCCGAAA GGCAGCAGAG AGTATAGCAG ACAACCCAAT
GAACACCACA CCTGGAATGG GACTTCGAGG AAACCCGACA GCGGGGCATG CCGACCCAAG
GACCGGGCGC CGGAGGGACG GAGGGACGCG CAGGCCGAGC GCGCGGCAGC CGCCAACGGC
CCCAAGAGGC GGTCCCCAGA GAAGCGGAGG GAGGGCACCC GCAGCGCCGA CAACACTTTG
GAGAGGAGGG AGAAGCACGA GAAGAGACGA GACGTCTCTC CGGAGCGGAG GCGAGAGCGG
TCACCCACCC GCAGGAGAGA CGGCTCCCCC AGCCGGCGGA GACGGTCTCT GGAGAGACTC
CTGGAGCAGA GGAGGTCCCC CGAGCGCAGG AGAGGGGGCT CGCCCGAGCG CAGGGCCAAG
TCCACCGACC GGAGGCGCGC ACGCTCCCCC GAGCGCAGGA GAGAGCGGTC CCTGGACAAA
AGGAACAGAG AGGACAGAGC CAGCCACCGA GAAAGGGAAG AGGCGAATCT GAAACAGGAT
GCCGGCAGAA GTTCCAGACA TCCCCCGGAG CAGAGAAGGC GACCTTACAA AGAATGTAGC
ACCGACCTCA GTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62635
Clone ID Related Accession (Same CDS sequence) XM_011534239
Accession Version XM_011534239.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X20
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACCTATGTCA CCTTCACCTG CGTCGGGATT GAGCAAGGGT
GAGAGAGAGA GAGAAATCAA TTCCACGAAT TTTGGAGAAT GTCCGATTCC AGATTACCAG
GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACTGGATTTG GATTTAGGAT TCTGGGTGGA
AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACT
GACGGCCGCC TGAGGTCTGG AGATGAATTA ATCTGTGTGG ATGGGACGCC AGTAATTGGA
AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCTG CCAAGCAAGG CCACGTCAAT
CTCACGGTGC GGCGTAAAGT GGTTTTTGCG GTGCCCAAAA CCGAGAACGA GGTGCCCTCG
CCAGCCTCCT CTCATCACAG TAGCAACCAG CCGGCCTCGC TGACAGAAGA GAAGCGCACT
CCGCAGGGCA GCCAGAACTC GCTGAACACG GTGAGCTCGG GCAGCGGCAG CACCAGCGGC
ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC GTGGTCAGCA CCGTGGTGCA GCCCTACGAC
GTGGAGATCC GGCGCGGGGA GAACGAGGGC TTCGGCTTCG TCATCGTGTC CTCGGTGAGC
AGGCCCGAAG CAGGCACGAC CTTTGCAGGC AATGCATGTG TGGCTATGCC TCACAAAATA
GGTCGGATTA TTGAGGGGAG CCCTGCTGAC CGCTGTGGCA AGCTGAAAGT AGGAGACCGG
ATCTTGGCAG TAAATGGATG TTCCATCACC AACAAATCCC ATTCAGACAT TGTGAACCTA
ATCAAGGAAG CGGGAAACAC AGTTACCCTC CGCATCATTC CTGGGGATGA GTCCTCGAAT
GCCACCTTGC TGACCAATGC AGAGAAGATT GCCACCATCA CCACCACACA CACCCCTTCT
CAGCAAGGGA CCCAGGAGAC AAGGAATACC ACCAAACCAA AGCAGGAATC TCAATTTGAG
TTCAAAGCAC CCCAAGCAAC ACAGGAGCAA GATTTTTACA CTGTGGAACT GGAAAGAGGA
GCCAAGGGAT TTGGCTTTAG CCTTCGAGGA GGCCGAGAGT ATAACATGGA CCTCTATGTT
CTGCGCTTAG CAGAGGACGG TCCTGCGGAG AGGTGTGGAA AGATGAGGAT TGGTGATGAA
ATTTTAGAGA TCAATGGTGA GACCACCAAA AACATGAAGC ATTCTCGAGC TATAGAACTG
ATTAAGAATG GTGGCCGCAG AGTTCGTCTG TTTCTGAAGC GGGGAGACGG CTCAGTACCA
GAATATGTTC TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532541.1
CDS529..4263
Misc Feature(1)571..828(+)
Misc Feature(2)607..801(+)
Misc Feature(3)892..1410(+)
Misc Feature(4)892..900(+)
Misc Feature(5)1438..1527(+)
Misc Feature(6)1480..1515(+)
Misc Feature(7)1618..1710(+)
Misc Feature(8)1660..1695(+)
Misc Feature(9)1960..2163(+)
Misc Feature(10)1975..2154(+)
Misc Feature(11)2455..2694(+)
Misc Feature(12)2488..2655(+)
Misc Feature(13)3073..3300(+)
Misc Feature(14)3082..3261(+)
Misc Feature(15)3520..3807(+)
Misc Feature(16)3556..3774(+)
Misc Feature(17)3985..4218(+)
Misc Feature(18)4015..4194(+)
Translation

Target ORF information:

RefSeq Version XM_011534239
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X20, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534239

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCAG CCGATTCTGG TGAACAAGAG
GAGCACACTC TCCAAGAAAC AGCATTACCA CCTGTGAATA GTAGCATCAT CGCTGCTCCC
ATCACGGACC CTTCTCAGAA GTTCCCTCAA TACCTACCTC TTTCTGCAGA GGATAATTTA
GGTCCTCTAC CTGAAAACTG GGAGATGGCC TATACTGAAA ATGGAGAAGT CTATTTTATA
GACCATAACA CGAAAACAAC ATCTTGGTTA GACCCTCGGT GCCTAAACAA GCAGCAGAAG
CCACTGGAAG AGTGTGAAGA TGATGAAGAA GGGGTACACA CCGAGGAGCT GGACAGTGAA
CTAGAACTGC CTGCTGGTTG GGAAAAGATT GAAGACCCTG TCTATGGTAT CTACTATGTA
GACCACATCA ACAGGAAGAC ACAATATGAG AACCCGGTTC TAGAAGCCAA ACGGAAGAAG
CAGCTTGAGC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG
CAGCAGCAGA CAGAAGAATG GACAGAAGAT CACTCAGCCC TTGTGCCTCC TGTTATTCCA
AACCACCCTC CAAGCAATCC AGAGCCAGCC AGAGAAGTTC CACTTCAGGG CAAACCCTTT
TTTACACGAA ACCCTTCTGA GTTGAAAGGC AAGTTCATTC ACACAAAGCT GCGGAAAAGC
AGTCGTGGCT TTGGCTTCAC GGTGGTTGGA GGGGATGAAC CTGATGAGTT TCTCCAGATC
AAGAGCTTGG TCCTAGATGG TCCTGCTGCA TTGGATGGCA AGATGGAAAC AGGGGATGTG
ATTGTAAGTG TGAATGACAC CTGTGTTTTG GGACACACAC ATGCTCAAGT TGTGAAGATC
TTCCAGTCCA TCCCCATTGG TGCCAGCGTG GACCTTGAAC TCTGCCGAGG TTATCCATTG
CCTTTTGATC CAGATGACCC CAATACAAGT TTAGTGACCT CGGTAGCCAT TTTGGATAAA
GAACCAATTA TTGTGAATGG GCAAGAGACC TATGATTCAC CAGCTAGCCA CAGTAGTAAA
ACAGGCAAAG TCAATGGCAT GAAGGATGCC AGGCCAAGCA GCCCAGCGGA CGTGGCTTCA
AATAGTTCTC ATGGTTATCC TAATGACACT GTTTCTTTGG CTTCCTCCAT AGCCACTCAG
CCAGAACTCA TAACTGTTCA TATTGTCAAA GGGCCAATGG GCTTTGGTTT TACTATCGCA
GACAGTCCTG GTGGGGGTGG CCAAAGAGTG AAACAGATTG TTGACAGCCC AAGGTGCCGA
GGCCTGAAAG AAGGGGATCT CATAGTGGAA GTTAATAAGA AGAACGTGCA GGCCCTAACT
CACAACCAAG TGGTGGATAT GCTGGTTGAG TGTCCTAAGG GAAGTGAGGT CACATTGTTG
GTGCAACGAG GAGGGCTGCC AGTTCCCAAG AAGAGCCCAA AGTCGCAACC ACTGGAAAGG
AAAGACAGCC AGAATAGTTC TCAGCACAGT GTTTCCAGCC ACCGAAGCCT GCACACAGCA
TCCCCAAGCC ACAGCACACA GGTGCTCCCC GAGTTCCCAC CTGCAGAGGC CCAAGCTCCA
GATCAAACTG ACAGCTCTGG CCAGAAAAAA CCAGATCCTT TTAAAATCTG GGCCCAGTCC
AGGAGCATGT ATGAAAACCG ACCTATGTCA CCTTCACCTG CGTCGGGATT GAGCAAGGGT
GAGAGAGAGA GAGAAATCAA TTCCACGAAT TTTGGAGAAT GTCCGATTCC AGATTACCAG
GAACAGGACA TCTTCCTCTG GAGAAAAGAG ACTGGATTTG GATTTAGGAT TCTGGGTGGA
AATGAACCAG GGGAACCTAT TTATATTGGT CACATCGTAC CACTGGGTGC TGCTGATACT
GACGGCCGCC TGAGGTCTGG AGATGAATTA ATCTGTGTGG ATGGGACGCC AGTAATTGGA
AAATCACACC AGCTTGTGGT CCAGCTTATG CAACAAGCTG CCAAGCAAGG CCACGTCAAT
CTCACGGTGC GGCGTAAAGT GGTTTTTGCG GTGCCCAAAA CCGAGAACGA GGTGCCCTCG
CCAGCCTCCT CTCATCACAG TAGCAACCAG CCGGCCTCGC TGACAGAAGA GAAGCGCACT
CCGCAGGGCA GCCAGAACTC GCTGAACACG GTGAGCTCGG GCAGCGGCAG CACCAGCGGC
ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC GTGGTCAGCA CCGTGGTGCA GCCCTACGAC
GTGGAGATCC GGCGCGGGGA GAACGAGGGC TTCGGCTTCG TCATCGTGTC CTCGGTGAGC
AGGCCCGAAG CAGGCACGAC CTTTGCAGGC AATGCATGTG TGGCTATGCC TCACAAAATA
GGTCGGATTA TTGAGGGGAG CCCTGCTGAC CGCTGTGGCA AGCTGAAAGT AGGAGACCGG
ATCTTGGCAG TAAATGGATG TTCCATCACC AACAAATCCC ATTCAGACAT TGTGAACCTA
ATCAAGGAAG CGGGAAACAC AGTTACCCTC CGCATCATTC CTGGGGATGA GTCCTCGAAT
GCCACCTTGC TGACCAATGC AGAGAAGATT GCCACCATCA CCACCACACA CACCCCTTCT
CAGCAAGGGA CCCAGGAGAC AAGGAATACC ACCAAACCAA AGCAGGAATC TCAATTTGAG
TTCAAAGCAC CCCAAGCAAC ACAGGAGCAA GATTTTTACA CTGTGGAACT GGAAAGAGGA
GCCAAGGGAT TTGGCTTTAG CCTTCGAGGA GGCCGAGAGT ATAACATGGA CCTCTATGTT
CTGCGCTTAG CAGAGGACGG TCCTGCGGAG AGGTGTGGAA AGATGAGGAT TGGTGATGAA
ATTTTAGAGA TCAATGGTGA GACCACCAAA AACATGAAGC ATTCTCGAGC TATAGAACTG
ATTAAGAATG GTGGCCGCAG AGTTCGTCTG TTTCTGAAGC GGGGAGACGG CTCAGTACCA
GAATATGTTC TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62636
Clone ID Related Accession (Same CDS sequence) XM_011534240
Accession Version XM_011534240.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X21
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTAGGT TGCCATGGAA CACCTGGGGA TTGTTCTTTA AGGAAAAGCA ACCACTGGAA 
AGGAAAGACA GCCAGAATAG TTCTCAGCAC AGTGTTTCCA GCCACCGAAG CCTGCACACA
GCATCCCCAA GCCACAGCAC ACAGGTGCTC CCCGAGTTCC CACCTGCAGA GGCCCAAGCT
CCAGATCAAA CTGACAGCTC TGGCCAGAAA AAACCAGATC CTTTTAAAAT CTGGGCCCAG
TCCAGGAGCA TGTATGAAAA CCGACCTATG TCACCTTCAC CTGCGTCGGG ATTGAGCAAG
GGTGAGAGAG AGAGAGAAAT CAATTCCACG AATTTTGGAG AATGTCCGAT TCCAGATTAC
CAGGAACAGG ACATCTTCCT CTGGAGAAAA GAGACTGGAT TTGGATTTAG GATTCTGGGT
GGAAATGAAC CAGGGGAACC TATTTATATT GGTCACATCG TACCACTGGG TGCTGCTGAT
ACTGACGGCC GCCTGAGGTC TGGAGATGAA TTAATCTGTG TGGATGGGAC GCCAGTAATT
GGAAAATCAC ACCAGCTTGT GGTCCAGCTT ATGCAACAAG CTGCCAAGCA AGGCCACGTC
AATCTCACGG TGCGGCGTAA AGTGGTTTTT GCGGTGCCCA AAACCGAGAA CGAGGTGCCC
TCGCCAGCCT CCTCTCATCA CAGTAGCAAC CAGCCGGCCT CGCTGACAGA AGAGAAGCGC
ACTCCGCAGG GCAGCCAGAA CTCGCTGAAC ACGGTGAGCT CGGGCAGCGG CAGCACCAGC
GGCATCGGCA GTGGCGGCGG CGGGGGCAGC GGCGTGGTCA GCACCGTGGT GCAGCCCTAC
GACGTGGAGA TCCGGCGCGG GGAGAACGAG GGCTTCGGCT TCGTCATCGT GTCCTCGGTG
AGCAGGCCCG AAGCAGGCAC GACCTTTGCA GGCAATGCAT GTGTGGCTAT GCCTCACAAA
ATAGGTCGGA TTATTGAGGG GAGCCCTGCT GACCGCTGTG GCAAGCTGAA AGTAGGAGAC
CGGATCTTGG CAGTAAATGG ATGTTCCATC ACCAACAAAT CCCATTCAGA CATTGTGAAC
CTAATCAAGG AAGCGGGAAA CACAGTTACC CTCCGCATCA TTCCTGGGGA TGAGTCCTCG
AATGCCACCT TGCTGACCAA TGCAGAGAAG ATTGCCACCA TCACCACCAC ACACACCCCT
TCTCAGCAAG GGACCCAGGA GACAAGGAAT ACCACCAAAC CAAAGCAGGA ATCTCAATTT
GAGTTCAAAG CACCCCAAGC AACACAGGAG CAAGATTTTT ACACTGTGGA ACTGGAAAGA
GGAGCCAAGG GATTTGGCTT TAGCCTTCGA GGAGGCCGAG AGTATAACAT GGACCTCTAT
GTTCTGCGCT TAGCAGAGGA CGGTCCTGCG GAGAGGTGTG GAAAGATGAG GATTGGTGAT
GAAATTTTAG AGATCAATGG TGAGACCACC AAAAACATGA AGCATTCTCG AGCTATAGAA
CTGATTAAGA ATGGTGGCCG CAGAGTTCGT CTGTTTCTGA AGCGGGGAGA CGGCTCAGTA
CCAGAATATG ACCCCAGCAG CGACCGCCAC GGCCCCGCCA CCGGTCCACA AGGTGTTCCG
GAAGTGAGGG CCGGGCCCGA CCGCCGGCAG CATCCGTCAT TGGAGTCCAG TTACCCACCC
GATCTTCACA AATCATCACC ACATGGGGAA AAGCGGGCAC ACGCAAGGGA TCCGAAAGGC
AGCAGAGAGT ATAGCAGACA ACCCAATGAA CACCACACCT GGAATGGGAC TTCGAGGAAA
CCCGACAGCG GGGCATGCCG ACCCAAGGAC CGGGCGCCGG AGGGACGGAG GGACGCGCAG
GCCGAGCGCG CGGCAGCCGC CAACGGCCCC AAGAGGCGGT CCCCAGAGAA GCGGAGGGAG
GGCACCCGCA GCGCCGACAA CACTTTGGAG AGGAGGGAGA AGCACGAGAA GAGACGAGAC
GTCTCTCCGG AGCGGAGGCG AGAGCGGTCA CCCACCCGCA GGAGAGACGG CTCCCCCAGC
CGGCGGAGAC GGTCTCTGGA GAGACTCCTG GAGCAGAGGA GGTCCCCCGA GCGCAGGAGA
GGGGGCTCGC CCGAGCGCAG GGCCAAGTCC ACCGACCGGA GGCGCGCACG CTCCCCCGAG
CGCAGGAGAG AGCGGTCCCT GGACAAAAGG AACAGAGAGG ACAGAGCCAG CCACCGAGAA
AGGGAAGAGG CGAATCTGAA ACAGGATGCC GGCAGAAGTT CCAGACATCC CCCGGAGCAG
AGAAGGCGAC CTTACAAAGA ATGTAGCACC GACCTCAGTA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532542.1
CDS294..2618
Misc Feature(1)681..908(+)
Misc Feature(2)690..869(+)
Misc Feature(3)1128..1415(+)
Misc Feature(4)1164..1382(+)
Misc Feature(5)1593..1826(+)
Misc Feature(6)1623..1802(+)
Translation

Target ORF information:

RefSeq Version XM_011534240
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X21, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534240

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTAGGT TGCCATGGAA CACCTGGGGA TTGTTCTTTA AGGAAAAGCA ACCACTGGAA 
AGGAAAGACA GCCAGAATAG TTCTCAGCAC AGTGTTTCCA GCCACCGAAG CCTGCACACA
GCATCCCCAA GCCACAGCAC ACAGGTGCTC CCCGAGTTCC CACCTGCAGA GGCCCAAGCT
CCAGATCAAA CTGACAGCTC TGGCCAGAAA AAACCAGATC CTTTTAAAAT CTGGGCCCAG
TCCAGGAGCA TGTATGAAAA CCGACCTATG TCACCTTCAC CTGCGTCGGG ATTGAGCAAG
GGTGAGAGAG AGAGAGAAAT CAATTCCACG AATTTTGGAG AATGTCCGAT TCCAGATTAC
CAGGAACAGG ACATCTTCCT CTGGAGAAAA GAGACTGGAT TTGGATTTAG GATTCTGGGT
GGAAATGAAC CAGGGGAACC TATTTATATT GGTCACATCG TACCACTGGG TGCTGCTGAT
ACTGACGGCC GCCTGAGGTC TGGAGATGAA TTAATCTGTG TGGATGGGAC GCCAGTAATT
GGAAAATCAC ACCAGCTTGT GGTCCAGCTT ATGCAACAAG CTGCCAAGCA AGGCCACGTC
AATCTCACGG TGCGGCGTAA AGTGGTTTTT GCGGTGCCCA AAACCGAGAA CGAGGTGCCC
TCGCCAGCCT CCTCTCATCA CAGTAGCAAC CAGCCGGCCT CGCTGACAGA AGAGAAGCGC
ACTCCGCAGG GCAGCCAGAA CTCGCTGAAC ACGGTGAGCT CGGGCAGCGG CAGCACCAGC
GGCATCGGCA GTGGCGGCGG CGGGGGCAGC GGCGTGGTCA GCACCGTGGT GCAGCCCTAC
GACGTGGAGA TCCGGCGCGG GGAGAACGAG GGCTTCGGCT TCGTCATCGT GTCCTCGGTG
AGCAGGCCCG AAGCAGGCAC GACCTTTGCA GGCAATGCAT GTGTGGCTAT GCCTCACAAA
ATAGGTCGGA TTATTGAGGG GAGCCCTGCT GACCGCTGTG GCAAGCTGAA AGTAGGAGAC
CGGATCTTGG CAGTAAATGG ATGTTCCATC ACCAACAAAT CCCATTCAGA CATTGTGAAC
CTAATCAAGG AAGCGGGAAA CACAGTTACC CTCCGCATCA TTCCTGGGGA TGAGTCCTCG
AATGCCACCT TGCTGACCAA TGCAGAGAAG ATTGCCACCA TCACCACCAC ACACACCCCT
TCTCAGCAAG GGACCCAGGA GACAAGGAAT ACCACCAAAC CAAAGCAGGA ATCTCAATTT
GAGTTCAAAG CACCCCAAGC AACACAGGAG CAAGATTTTT ACACTGTGGA ACTGGAAAGA
GGAGCCAAGG GATTTGGCTT TAGCCTTCGA GGAGGCCGAG AGTATAACAT GGACCTCTAT
GTTCTGCGCT TAGCAGAGGA CGGTCCTGCG GAGAGGTGTG GAAAGATGAG GATTGGTGAT
GAAATTTTAG AGATCAATGG TGAGACCACC AAAAACATGA AGCATTCTCG AGCTATAGAA
CTGATTAAGA ATGGTGGCCG CAGAGTTCGT CTGTTTCTGA AGCGGGGAGA CGGCTCAGTA
CCAGAATATG ACCCCAGCAG CGACCGCCAC GGCCCCGCCA CCGGTCCACA AGGTGTTCCG
GAAGTGAGGG CCGGGCCCGA CCGCCGGCAG CATCCGTCAT TGGAGTCCAG TTACCCACCC
GATCTTCACA AATCATCACC ACATGGGGAA AAGCGGGCAC ACGCAAGGGA TCCGAAAGGC
AGCAGAGAGT ATAGCAGACA ACCCAATGAA CACCACACCT GGAATGGGAC TTCGAGGAAA
CCCGACAGCG GGGCATGCCG ACCCAAGGAC CGGGCGCCGG AGGGACGGAG GGACGCGCAG
GCCGAGCGCG CGGCAGCCGC CAACGGCCCC AAGAGGCGGT CCCCAGAGAA GCGGAGGGAG
GGCACCCGCA GCGCCGACAA CACTTTGGAG AGGAGGGAGA AGCACGAGAA GAGACGAGAC
GTCTCTCCGG AGCGGAGGCG AGAGCGGTCA CCCACCCGCA GGAGAGACGG CTCCCCCAGC
CGGCGGAGAC GGTCTCTGGA GAGACTCCTG GAGCAGAGGA GGTCCCCCGA GCGCAGGAGA
GGGGGCTCGC CCGAGCGCAG GGCCAAGTCC ACCGACCGGA GGCGCGCACG CTCCCCCGAG
CGCAGGAGAG AGCGGTCCCT GGACAAAAGG AACAGAGAGG ACAGAGCCAG CCACCGAGAA
AGGGAAGAGG CGAATCTGAA ACAGGATGCC GGCAGAAGTT CCAGACATCC CCCGGAGCAG
AGAAGGCGAC CTTACAAAGA ATGTAGCACC GACCTCAGTA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62637
Clone ID Related Accession (Same CDS sequence) XM_011534241
Accession Version XM_011534241.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X22
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022517.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCTAGGT TGCCATGGAA CACCTGGGGA TTGTTCTTTA AGGAAAAGCA ACCACTGGAA 
AGGAAAGACA GCCAGAATAG TTCTCAGCAC AGTGTTTCCA GCCACCGAAG CCTGCACACA
GCATCCCCAA GCCACAGCAC ACAGGTGCTC CCCGAGTTCC CACCTGCAGA GGCCCAAGCT
CCAGATCAAA CTGACAGCTC TGGCCAGAAA AAACCAGATC CTTTTAAAAT CTGGGCCCAG
TCCAGGAGCA TGTATGAAAA CCGACTTCCA GATTACCAGG AACAGGACAT CTTCCTCTGG
AGAAAAGAGA CTGGATTTGG ATTTAGGATT CTGGGTGGAA ATGAACCAGG GGAACCTATT
TATATTGGTC ACATCGTACC ACTGGGTGCT GCTGATACTG ACGGCCGCCT GAGGTCTGGA
GATGAATTAA TCTGTGTGGA TGGGACGCCA GTAATTGGAA AATCACACCA GCTTGTGGTC
CAGCTTATGC AACAAGCTGC CAAGCAAGGC CACGTCAATC TCACGGTGCG GCGTAAAGTG
GTTTTTGCGG TGCCCAAAAC CGAGAACGAG GTGCCCTCGC CAGCCTCCTC TCATCACAGT
AGCAACCAGC CGGCCTCGCT GACAGAAGAG AAGCGCACTC CGCAGGGCAG CCAGAACTCG
CTGAACACGG TGAGCTCGGG CAGCGGCAGC ACCAGCGGCA TCGGCAGTGG CGGCGGCGGG
GGCAGCGGCG TGGTCAGCAC CGTGGTGCAG CCCTACGACG TGGAGATCCG GCGCGGGGAG
AACGAGGGCT TCGGCTTCGT CATCGTGTCC TCGGTGAGCA GGCCCGAAGC AGGCACGACC
TTTGCAGGCA ATGCATGTGT GGCTATGCCT CACAAAATAG GTCGGATTAT TGAGGGGAGC
CCTGCTGACC GCTGTGGCAA GCTGAAAGTA GGAGACCGGA TCTTGGCAGT AAATGGATGT
TCCATCACCA ACAAATCCCA TTCAGACATT GTGAACCTAA TCAAGGAAGC GGGAAACACA
GTTACCCTCC GCATCATTCC TGGGGATGAG TCCTCGAATG CCACCTTGCT GACCAATGCA
GAGAAGATTG CCACCATCAC CACCACACAC ACCCCTTCTC AGCAAGGGAC CCAGGAGACA
AGGAATACCA CCAAACCAAA GCAGGAATCT CAATTTGAGT TCAAAGCACC CCAAGCAACA
CAGGAGCAAG ATTTTTACAC TGTGGAACTG GAAAGAGGAG CCAAGGGATT TGGCTTTAGC
CTTCGAGGAG GCCGAGAGTA TAACATGGAC CTCTATGTTC TGCGCTTAGC AGAGGACGGT
CCTGCGGAGA GGTGTGGAAA GATGAGGATT GGTGATGAAA TTTTAGAGAT CAATGGTGAG
ACCACCAAAA ACATGAAGCA TTCTCGAGCT ATAGAACTGA TTAAGAATGG TGGCCGCAGA
GTTCGTCTGT TTCTGAAGCG GGGAGACGGC TCAGTACCAG AATATGACCC CAGCAGCGAC
CGCCACGGCC CCGCCACCGG TCCACAAGGT GTTCCGGAAG TGAGGGCCGG GCCCGACCGC
CGGCAGCATC CGTCATTGGA GTCCAGTTAC CCACCCGATC TTCACAAATC ATCACCACAT
GGGGAAAAGC GGGCACACGC AAGGGATCCG AAAGGCAGCA GAGAGTATAG CAGACAACCC
AATGAACACC ACACCTGGAA TGGGACTTCG AGGAAACCCG ACAGCGGGGC ATGCCGACCC
AAGGACCGGG CGCCGGAGGG ACGGAGGGAC GCGCAGGCCG AGCGCGCGGC AGCCGCCAAC
GGCCCCAAGA GGCGGTCCCC AGAGAAGCGG AGGGAGGGCA CCCGCAGCGC CGACAACACT
TTGGAGAGGA GGGAGAAGCA CGAGAAGAGA CGAGACGTCT CTCCGGAGCG GAGGCGAGAG
CGGTCACCCA CCCGCAGGAG AGACGGCTCC CCCAGCCGGC GGAGACGGTC TCTGGAGAGA
CTCCTGGAGC AGAGGAGGTC CCCCGAGCGC AGGAGAGGGG GCTCGCCCGA GCGCAGGGCC
AAGTCCACCG ACCGGAGGCG CGCACGCTCC CCCGAGCGCA GGAGAGAGCG GTCCCTGGAC
AAAAGGAACA GAGAGGACAG AGCCAGCCAC CGAGAAAGGG AAGAGGCGAA TCTGAAACAG
GATGCCGGCA GAAGTTCCAG ACATCCCCCG GAGCAGAGAA GGCGACCTTA CAAAGAATGT
AGCACCGACC TCAGTATCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532543.1
CDS286..2526
Misc Feature(1)589..816(+)
Misc Feature(2)598..777(+)
Misc Feature(3)1036..1323(+)
Misc Feature(4)1072..1290(+)
Misc Feature(5)1501..1734(+)
Misc Feature(6)1531..1710(+)
Translation

Target ORF information:

RefSeq Version XM_011534241
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant X22, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534241

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCTAGGT TGCCATGGAA CACCTGGGGA TTGTTCTTTA AGGAAAAGCA ACCACTGGAA 
AGGAAAGACA GCCAGAATAG TTCTCAGCAC AGTGTTTCCA GCCACCGAAG CCTGCACACA
GCATCCCCAA GCCACAGCAC ACAGGTGCTC CCCGAGTTCC CACCTGCAGA GGCCCAAGCT
CCAGATCAAA CTGACAGCTC TGGCCAGAAA AAACCAGATC CTTTTAAAAT CTGGGCCCAG
TCCAGGAGCA TGTATGAAAA CCGACTTCCA GATTACCAGG AACAGGACAT CTTCCTCTGG
AGAAAAGAGA CTGGATTTGG ATTTAGGATT CTGGGTGGAA ATGAACCAGG GGAACCTATT
TATATTGGTC ACATCGTACC ACTGGGTGCT GCTGATACTG ACGGCCGCCT GAGGTCTGGA
GATGAATTAA TCTGTGTGGA TGGGACGCCA GTAATTGGAA AATCACACCA GCTTGTGGTC
CAGCTTATGC AACAAGCTGC CAAGCAAGGC CACGTCAATC TCACGGTGCG GCGTAAAGTG
GTTTTTGCGG TGCCCAAAAC CGAGAACGAG GTGCCCTCGC CAGCCTCCTC TCATCACAGT
AGCAACCAGC CGGCCTCGCT GACAGAAGAG AAGCGCACTC CGCAGGGCAG CCAGAACTCG
CTGAACACGG TGAGCTCGGG CAGCGGCAGC ACCAGCGGCA TCGGCAGTGG CGGCGGCGGG
GGCAGCGGCG TGGTCAGCAC CGTGGTGCAG CCCTACGACG TGGAGATCCG GCGCGGGGAG
AACGAGGGCT TCGGCTTCGT CATCGTGTCC TCGGTGAGCA GGCCCGAAGC AGGCACGACC
TTTGCAGGCA ATGCATGTGT GGCTATGCCT CACAAAATAG GTCGGATTAT TGAGGGGAGC
CCTGCTGACC GCTGTGGCAA GCTGAAAGTA GGAGACCGGA TCTTGGCAGT AAATGGATGT
TCCATCACCA ACAAATCCCA TTCAGACATT GTGAACCTAA TCAAGGAAGC GGGAAACACA
GTTACCCTCC GCATCATTCC TGGGGATGAG TCCTCGAATG CCACCTTGCT GACCAATGCA
GAGAAGATTG CCACCATCAC CACCACACAC ACCCCTTCTC AGCAAGGGAC CCAGGAGACA
AGGAATACCA CCAAACCAAA GCAGGAATCT CAATTTGAGT TCAAAGCACC CCAAGCAACA
CAGGAGCAAG ATTTTTACAC TGTGGAACTG GAAAGAGGAG CCAAGGGATT TGGCTTTAGC
CTTCGAGGAG GCCGAGAGTA TAACATGGAC CTCTATGTTC TGCGCTTAGC AGAGGACGGT
CCTGCGGAGA GGTGTGGAAA GATGAGGATT GGTGATGAAA TTTTAGAGAT CAATGGTGAG
ACCACCAAAA ACATGAAGCA TTCTCGAGCT ATAGAACTGA TTAAGAATGG TGGCCGCAGA
GTTCGTCTGT TTCTGAAGCG GGGAGACGGC TCAGTACCAG AATATGACCC CAGCAGCGAC
CGCCACGGCC CCGCCACCGG TCCACAAGGT GTTCCGGAAG TGAGGGCCGG GCCCGACCGC
CGGCAGCATC CGTCATTGGA GTCCAGTTAC CCACCCGATC TTCACAAATC ATCACCACAT
GGGGAAAAGC GGGCACACGC AAGGGATCCG AAAGGCAGCA GAGAGTATAG CAGACAACCC
AATGAACACC ACACCTGGAA TGGGACTTCG AGGAAACCCG ACAGCGGGGC ATGCCGACCC
AAGGACCGGG CGCCGGAGGG ACGGAGGGAC GCGCAGGCCG AGCGCGCGGC AGCCGCCAAC
GGCCCCAAGA GGCGGTCCCC AGAGAAGCGG AGGGAGGGCA CCCGCAGCGC CGACAACACT
TTGGAGAGGA GGGAGAAGCA CGAGAAGAGA CGAGACGTCT CTCCGGAGCG GAGGCGAGAG
CGGTCACCCA CCCGCAGGAG AGACGGCTCC CCCAGCCGGC GGAGACGGTC TCTGGAGAGA
CTCCTGGAGC AGAGGAGGTC CCCCGAGCGC AGGAGAGGGG GCTCGCCCGA GCGCAGGGCC
AAGTCCACCG ACCGGAGGCG CGCACGCTCC CCCGAGCGCA GGAGAGAGCG GTCCCTGGAC
AAAAGGAACA GAGAGGACAG AGCCAGCCAC CGAGAAAGGG AAGAGGCGAA TCTGAAACAG
GATGCCGGCA GAAGTTCCAG ACATCCCCCG GAGCAGAGAA GGCGACCTTA CAAAGAATGT
AGCACCGACC TCAGTATCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24845
Clone ID Related Accession (Same CDS sequence) NM_004742
Accession Version NM_004742.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3771bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC062367.1, AB010894.1 and AC121493.1. On May 19, 2005 this sequence version replaced gi:9257194. Transcript Variant: This variant (2) differs in the 3' UTR and the 3' coding region, compared to variant 1. The resulting isoform (b) contains a distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB010894.1, AK289803.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACCTAT GTCACCTTCA CCTGCGTCGG GATTGAGCAA GGGTGAGAGA
GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCCGA TTCCAGATTA CCAGGAACAG
GACATCTTCC TCTGGAGAAA AGAGACTGGA TTTGGATTTA GGATTCTGGG TGGAAATGAA
CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACTGACGGC
CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA CGCCAGTAAT TGGAAAATCA
CACCAGCTTG TGGTCCAGCT TATGCAACAA GCTGCCAAGC AAGGCCACGT CAATCTCACG
GTGCGGCGTA AAGTGGTTTT TGCGGTGCCC AAAACCGAGA ACGAGGTGCC CTCGCCAGCC
TCCTCTCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACAG AAGAGAAGCG CACTCCGCAG
GGCAGCCAGA ACTCGCTGAA CACGGTGAGC TCGGGCAGCG GCAGCACCAG CGGCATCGGC
AGTGGCGGCG GCGGGGGCAG CGGCGTGGTC AGCACCGTGG TGCAGCCCTA CGACGTGGAG
ATCCGGCGCG GGGAGAACGA GGGCTTCGGC TTCGTCATCG TGTCCTCGGT GAGCAGGCCC
GAAGCAGGCA CGACCTTTGC AGGCAATGCA TGTGTGGCTA TGCCTCACAA AATAGGTCGG
ATTATTGAGG GGAGCCCTGC TGACCGCTGT GGCAAGCTGA AAGTAGGAGA CCGGATCTTG
GCAGTAAATG GATGTTCCAT CACCAACAAA TCCCATTCAG ACATTGTGAA CCTAATCAAG
GAAGCGGGAA ACACAGTTAC CCTCCGCATC ATTCCTGGGG ATGAGTCCTC GAATGCCACC
TTGCTGACCA ATGCAGAGAA GATTGCCACC ATCACCACCA CACACACCCC TTCTCAGCAA
GGGACCCAGG AGACAAGGAA TACCACCAAA CCAAAGCAGG AATCTCAATT TGAGTTCAAA
GCACCCCAAG CAACACAGGA GCAAGATTTT TACACTGTGG AACTGGAAAG AGGAGCCAAG
GGATTTGGCT TTAGCCTTCG AGGAGGCCGA GAGTATAACA TGGACCTCTA TGTTCTGCGC
TTAGCAGAGG ACGGTCCTGC GGAGAGGTGT GGAAAGATGA GGATTGGTGA TGAAATTTTA
GAGATCAATG GTGAGACCAC CAAAAACATG AAGCATTCTC GAGCTATAGA ACTGATTAAG
AATGGTGGCC GCAGAGTTCG TCTGTTTCTG AAGCGGGGAG ACGGCTCAGT ACCAGAATAT
GGTGGGTCAA ACTATGAAAA CATTCCTTCC TTCCCTGGCA TGACTCCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004733.2
CDS527..4297
Misc Feature(1)518..520(+)
Misc Feature(2)569..826(+)
Misc Feature(3)605..799(+)
Misc Feature(4)890..1405(+)
Misc Feature(5)890..898(+)
Misc Feature(6)1433..1522(+)
Misc Feature(7)1475..1510(+)
Misc Feature(8)1610..1702(+)
Misc Feature(9)1652..1687(+)
Misc Feature(10)1952..2155(+)
Misc Feature(11)1967..2146(+)
Misc Feature(12)2447..2686(+)
Misc Feature(13)2480..2647(+)
Misc Feature(14)2714..2716(+)
Misc Feature(15)2747..2749(+)
Misc Feature(16)3065..3292(+)
Misc Feature(17)3074..3253(+)
Misc Feature(18)3434..3724(+)
Misc Feature(19)3512..3799(+)
Misc Feature(20)3548..3766(+)
Misc Feature(21)3977..4210(+)
Misc Feature(22)4007..4186(+)
Exon (1)1..839
Gene:MAGI1
Gene Synonym:
Exon (2)840..956
Gene:MAGI1
Gene Synonym:
Exon (3)957..1076
Gene:MAGI1
Gene Synonym:
Exon (4)1077..1283
Gene:MAGI1
Gene Synonym:
Exon (5)1284..1485
Gene:MAGI1
Gene Synonym:
Exon (6)1486..1568
Gene:MAGI1
Gene Synonym:
Exon (7)1569..1604
Gene:MAGI1
Gene Synonym:
Exon (8)1605..1662
Gene:MAGI1
Gene Synonym:
Exon (9)1663..1796
Gene:MAGI1
Gene Synonym:
Exon (10)1797..1889
Gene:MAGI1
Gene Synonym:
Exon (11)1890..2072
Gene:MAGI1
Gene Synonym:
Exon (12)2073..2693
Gene:MAGI1
Gene Synonym:
Exon (13)2694..2725
Gene:MAGI1
Gene Synonym:
Exon (14)2726..2942
Gene:MAGI1
Gene Synonym:
Exon (15)2943..3026
Gene:MAGI1
Gene Synonym:
Exon (16)3027..3118
Gene:MAGI1
Gene Synonym:
Exon (17)3119..3311
Gene:MAGI1
Gene Synonym:
Exon (18)3312..3605
Gene:MAGI1
Gene Synonym:
Exon (19)3606..3809
Gene:MAGI1
Gene Synonym:
Exon (20)3810..3903
Gene:MAGI1
Gene Synonym:
Exon (21)3904..3964
Gene:MAGI1
Gene Synonym:
Exon (22)3965..4108
Gene:MAGI1
Gene Synonym:
Exon (23)4109..6178
Gene:MAGI1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004742
Organism Homo sapiens (human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004742

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACCTAT GTCACCTTCA CCTGCGTCGG GATTGAGCAA GGGTGAGAGA
GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCCGA TTCCAGATTA CCAGGAACAG
GACATCTTCC TCTGGAGAAA AGAGACTGGA TTTGGATTTA GGATTCTGGG TGGAAATGAA
CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACTGACGGC
CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA CGCCAGTAAT TGGAAAATCA
CACCAGCTTG TGGTCCAGCT TATGCAACAA GCTGCCAAGC AAGGCCACGT CAATCTCACG
GTGCGGCGTA AAGTGGTTTT TGCGGTGCCC AAAACCGAGA ACGAGGTGCC CTCGCCAGCC
TCCTCTCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACAG AAGAGAAGCG CACTCCGCAG
GGCAGCCAGA ACTCGCTGAA CACGGTGAGC TCGGGCAGCG GCAGCACCAG CGGCATCGGC
AGTGGCGGCG GCGGGGGCAG CGGCGTGGTC AGCACCGTGG TGCAGCCCTA CGACGTGGAG
ATCCGGCGCG GGGAGAACGA GGGCTTCGGC TTCGTCATCG TGTCCTCGGT GAGCAGGCCC
GAAGCAGGCA CGACCTTTGC AGGCAATGCA TGTGTGGCTA TGCCTCACAA AATAGGTCGG
ATTATTGAGG GGAGCCCTGC TGACCGCTGT GGCAAGCTGA AAGTAGGAGA CCGGATCTTG
GCAGTAAATG GATGTTCCAT CACCAACAAA TCCCATTCAG ACATTGTGAA CCTAATCAAG
GAAGCGGGAA ACACAGTTAC CCTCCGCATC ATTCCTGGGG ATGAGTCCTC GAATGCCACC
TTGCTGACCA ATGCAGAGAA GATTGCCACC ATCACCACCA CACACACCCC TTCTCAGCAA
GGGACCCAGG AGACAAGGAA TACCACCAAA CCAAAGCAGG AATCTCAATT TGAGTTCAAA
GCACCCCAAG CAACACAGGA GCAAGATTTT TACACTGTGG AACTGGAAAG AGGAGCCAAG
GGATTTGGCT TTAGCCTTCG AGGAGGCCGA GAGTATAACA TGGACCTCTA TGTTCTGCGC
TTAGCAGAGG ACGGTCCTGC GGAGAGGTGT GGAAAGATGA GGATTGGTGA TGAAATTTTA
GAGATCAATG GTGAGACCAC CAAAAACATG AAGCATTCTC GAGCTATAGA ACTGATTAAG
AATGGTGGCC GCAGAGTTCG TCTGTTTCTG AAGCGGGGAG ACGGCTCAGT ACCAGAATAT
GGTGGGTCAA ACTATGAAAA CATTCCTTCC TTCCCTGGCA TGACTCCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24816
Clone ID Related Accession (Same CDS sequence) NM_015520
Accession Version NM_015520.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC062367.1, AF401654.1, AK023358.1 and AC121493.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript, and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF401654.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACCTAT GTCACCTTCA CCTGCGTCGG GATTGAGCAA GGGTGAGAGA
GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCCGA TTCCAGATTA CCAGGAACAG
GACATCTTCC TCTGGAGAAA AGAGACTGGA TTTGGATTTA GGATTCTGGG TGGAAATGAA
CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACTGACGGC
CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA CGCCAGTAAT TGGAAAATCA
CACCAGCTTG TGGTCCAGCT TATGCAACAA GCTGCCAAGC AAGGCCACGT CAATCTCACG
GTGCGGCGTA AAGTGGTTTT TGCGGTGCCC AAAACCGAGA ACGAGGTGCC CTCGCCAGCC
TCCTCTCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACAG AAGAGAAGCG CACTCCGCAG
GGCAGCCAGA ACTCGCTGAA CACGGTGAGC TCGGGCAGCG GCAGCACCAG CGGCATCGGC
AGTGGCGGCG GCGGGGGCAG CGGCGTGGTC AGCACCGTGG TGCAGCCCTA CGACGTGGAG
ATCCGGCGCG GGGAGAACGA GGGCTTCGGC TTCGTCATCG TGTCCTCGGT GAGCAGGCCC
GAAGCAGGCA CGACCTTTGG CAATGCATGT GTGGCTATGC CTCACAAAAT AGGTCGGATT
ATTGAGGGGA GCCCTGCTGA CCGCTGTGGC AAGCTGAAAG TAGGAGACCG GATCTTGGCA
GTAAATGGAT GTTCCATCAC CAACAAATCC CATTCAGACA TTGTGAACCT AATCAAGGAA
GCGGGAAACA CAGTTACCCT CCGCATCATT CCTGGGGATG AGTCCTCGAA TGCCACCTTG
CTGACCAATG CAGAGAAGAT TGCCACCATC ACCACCACAC ACACCCCTTC TCAGCAAGGG
ACCCAGGAGA CAAGGAATAC CACCAAACCA AAGCAGGAAT CTCAATTTGA GTTCAAAGCA
CCCCAAGCAA CACAGGAGCA AGATTTTTAC ACTGTGGAAC TGGAAAGAGG AGCCAAGGGA
TTTGGCTTTA GCCTTCGAGG AGGCCGAGAG TATAACATGG ACCTCTATGT TCTGCGCTTA
GCAGAGGACG GTCCTGCGGA GAGGTGTGGA AAGATGAGGA TTGGTGATGA AATTTTAGAG
ATCAATGGTG AGACCACCAA AAACATGAAG CATTCTCGAG CTATAGAACT GATTAAGAAT
GGTGGCCGCA GAGTTCGTCT GTTTCTGAAG CGGGGAGACG GCTCAGTACC AGAATATGCG
ATGATACCTC CTAATATCGC TGCATGTATG AGAAATGAAA AGCTCGGGGA GGCTTGCTTC
TACCTTATGG GCCATAATCA AACTACGACC CCAGCAGCGA CCGCCACGGC CCCGCCACCG
GTCCACAAGG TGTTCCGGAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_056335.1
CDS527..4390
Misc Feature(1)518..520(+)
Misc Feature(2)569..826(+)
Misc Feature(3)605..799(+)
Misc Feature(4)890..1405(+)
Misc Feature(5)890..898(+)
Misc Feature(6)1433..1522(+)
Misc Feature(7)1475..1510(+)
Misc Feature(8)1610..1702(+)
Misc Feature(9)1652..1687(+)
Misc Feature(10)1952..2155(+)
Misc Feature(11)1967..2146(+)
Misc Feature(12)2447..2686(+)
Misc Feature(13)2480..2647(+)
Misc Feature(14)2714..2716(+)
Misc Feature(15)2747..2749(+)
Misc Feature(16)3065..3292(+)
Misc Feature(17)3074..3253(+)
Misc Feature(18)3512..3796(+)
Misc Feature(19)3548..3763(+)
Misc Feature(20)3974..4207(+)
Misc Feature(21)4004..4183(+)
Exon (1)1..839
Gene:MAGI1
Gene Synonym:
Exon (2)840..956
Gene:MAGI1
Gene Synonym:
Exon (3)957..1076
Gene:MAGI1
Gene Synonym:
Exon (4)1077..1283
Gene:MAGI1
Gene Synonym:
Exon (5)1284..1485
Gene:MAGI1
Gene Synonym:
Exon (6)1486..1568
Gene:MAGI1
Gene Synonym:
Exon (7)1569..1604
Gene:MAGI1
Gene Synonym:
Exon (8)1605..1662
Gene:MAGI1
Gene Synonym:
Exon (9)1663..1796
Gene:MAGI1
Gene Synonym:
Exon (10)1797..1889
Gene:MAGI1
Gene Synonym:
Exon (11)1890..2072
Gene:MAGI1
Gene Synonym:
Exon (12)2073..2693
Gene:MAGI1
Gene Synonym:
Exon (13)2694..2725
Gene:MAGI1
Gene Synonym:
Exon (14)2726..2942
Gene:MAGI1
Gene Synonym:
Exon (15)2943..3026
Gene:MAGI1
Gene Synonym:
Exon (16)3027..3118
Gene:MAGI1
Gene Synonym:
Exon (17)3119..3311
Gene:MAGI1
Gene Synonym:
Exon (18)3312..3605
Gene:MAGI1
Gene Synonym:
Exon (19)3606..3806
Gene:MAGI1
Gene Synonym:
Exon (20)3807..3900
Gene:MAGI1
Gene Synonym:
Exon (21)3901..3961
Gene:MAGI1
Gene Synonym:
Exon (22)3962..4105
Gene:MAGI1
Gene Synonym:
Exon (23)4106..4244
Gene:MAGI1
Gene Synonym:
Exon (24)4245..4333
Gene:MAGI1
Gene Synonym:
Exon (25)4334..7235
Gene:MAGI1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_015520
Organism Homo sapiens (human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_015520

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACCTAT GTCACCTTCA CCTGCGTCGG GATTGAGCAA GGGTGAGAGA
GAGAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCCGA TTCCAGATTA CCAGGAACAG
GACATCTTCC TCTGGAGAAA AGAGACTGGA TTTGGATTTA GGATTCTGGG TGGAAATGAA
CCAGGGGAAC CTATTTATAT TGGTCACATC GTACCACTGG GTGCTGCTGA TACTGACGGC
CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA CGCCAGTAAT TGGAAAATCA
CACCAGCTTG TGGTCCAGCT TATGCAACAA GCTGCCAAGC AAGGCCACGT CAATCTCACG
GTGCGGCGTA AAGTGGTTTT TGCGGTGCCC AAAACCGAGA ACGAGGTGCC CTCGCCAGCC
TCCTCTCATC ACAGTAGCAA CCAGCCGGCC TCGCTGACAG AAGAGAAGCG CACTCCGCAG
GGCAGCCAGA ACTCGCTGAA CACGGTGAGC TCGGGCAGCG GCAGCACCAG CGGCATCGGC
AGTGGCGGCG GCGGGGGCAG CGGCGTGGTC AGCACCGTGG TGCAGCCCTA CGACGTGGAG
ATCCGGCGCG GGGAGAACGA GGGCTTCGGC TTCGTCATCG TGTCCTCGGT GAGCAGGCCC
GAAGCAGGCA CGACCTTTGG CAATGCATGT GTGGCTATGC CTCACAAAAT AGGTCGGATT
ATTGAGGGGA GCCCTGCTGA CCGCTGTGGC AAGCTGAAAG TAGGAGACCG GATCTTGGCA
GTAAATGGAT GTTCCATCAC CAACAAATCC CATTCAGACA TTGTGAACCT AATCAAGGAA
GCGGGAAACA CAGTTACCCT CCGCATCATT CCTGGGGATG AGTCCTCGAA TGCCACCTTG
CTGACCAATG CAGAGAAGAT TGCCACCATC ACCACCACAC ACACCCCTTC TCAGCAAGGG
ACCCAGGAGA CAAGGAATAC CACCAAACCA AAGCAGGAAT CTCAATTTGA GTTCAAAGCA
CCCCAAGCAA CACAGGAGCA AGATTTTTAC ACTGTGGAAC TGGAAAGAGG AGCCAAGGGA
TTTGGCTTTA GCCTTCGAGG AGGCCGAGAG TATAACATGG ACCTCTATGT TCTGCGCTTA
GCAGAGGACG GTCCTGCGGA GAGGTGTGGA AAGATGAGGA TTGGTGATGA AATTTTAGAG
ATCAATGGTG AGACCACCAA AAACATGAAG CATTCTCGAG CTATAGAACT GATTAAGAAT
GGTGGCCGCA GAGTTCGTCT GTTTCTGAAG CGGGGAGACG GCTCAGTACC AGAATATGCG
ATGATACCTC CTAATATCGC TGCATGTATG AGAAATGAAA AGCTCGGGGA GGCTTGCTTC
TACCTTATGG GCCATAATCA AACTACGACC CCAGCAGCGA CCGCCACGGC CCCGCCACCG
GTCCACAAGG TGTTCCGGAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24988
Clone ID Related Accession (Same CDS sequence) NM_001033057
Accession Version NM_001033057.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4389bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC062367.1, AF401654.1, AK023358.1 and AC121493.1. Transcript Variant: This variant (3) lacks two segments in the coding region, one of which leads to a frameshift, compared to variant 1. The resulting isoform (c) contains a shorter and distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF401656.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACTTCC AGATTACCAG GAACAGGACA TCTTCCTCTG GAGAAAAGAG
ACTGGATTTG GATTTAGGAT TCTGGGTGGA AATGAACCAG GGGAACCTAT TTATATTGGT
CACATCGTAC CACTGGGTGC TGCTGATACT GACGGCCGCC TGAGGTCTGG AGATGAATTA
ATCTGTGTGG ATGGGACGCC AGTAATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
CAACAAGCTG CCAAGCAAGG CCACGTCAAT CTCACGGTGC GGCGTAAAGT GGTTTTTGCG
GTGCCCAAAA CCGAGAACGA GGTGCCCTCG CCAGCCTCCT CTCATCACAG TAGCAACCAG
CCGGCCTCGC TGACAGAAGA GAAGCGCACT CCGCAGGGCA GCCAGAACTC GCTGAACACG
GTGAGCTCGG GCAGCGGCAG CACCAGCGGC ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC
GTGGTCAGCA CCGTGGTGCA GCCCTACGAC GTGGAGATCC GGCGCGGGGA GAACGAGGGC
TTCGGCTTCG TCATCGTGTC CTCGGTGAGC AGGCCCGAAG CAGGCACGAC CTTTGGCAAT
GCATGTGTGG CTATGCCTCA CAAAATAGGT CGGATTATTG AGGGGAGCCC TGCTGACCGC
TGTGGCAAGC TGAAAGTAGG AGACCGGATC TTGGCAGTAA ATGGATGTTC CATCACCAAC
AAATCCCATT CAGACATTGT GAACCTAATC AAGGAAGCGG GAAACACAGT TACCCTCCGC
ATCATTCCTG GGGATGAGTC CTCGAATGCC ACCTTGCTGA CCAATGCAGA GAAGATTGCC
ACCATCACCA CCACACACAC CCCTTCTCAG CAAGGGACCC AGGAGACAAG GAATACCACC
AAACCAAAGC AGGAATCTCA ATTTGAGTTC AAAGCACCCC AAGCAACACA GGAGCAAGAT
TTTTACACTG TGGAACTGGA AAGAGGAGCC AAGGGATTTG GCTTTAGCCT TCGAGGAGGC
CGAGAGTATA ACATGGACCT CTATGTTCTG CGCTTAGCAG AGGACGGTCC TGCGGAGAGG
TGTGGAAAGA TGAGGATTGG TGATGAAATT TTAGAGATCA ATGGTGAGAC CACCAAAAAC
ATGAAGCATT CTCGAGCTAT AGAACTGATT AAGAATGGTG GCCGCAGAGT TCGTCTGTTT
CTGAAGCGGG GAGACGGCTC AGTACCAGAA TATGACCCCA GCAGCGACCG CCACGGCCCC
GCCACCGGTC CACAAGGTGT TCCGGAAGTG AGGGCCGGGC CCGACCGCCG GCAGCATCCG
TCATTGGAGT CCAGTTACCC ACCCGATCTT CACAAATCAT CACCACATGG GGAAAAGCGG
GCACACGCAA GGGATCCGAA AGGCAGCAGA GAGTATAGCA GACAACCCAA TGAACACCAC
ACCTGGAATG GGACTTCGAG GAAACCCGAC AGCGGGGCAT GCCGACCCAA GGACCGGGCG
CCGGAGGGAC GGAGGGACGC GCAGGCCGAG CGCGCGGCAG CCGCCAACGG CCCCAAGAGG
CGGTCCCCAG AGAAGCGGAG GGAGGGCACC CGCAGCGCCG ACAACACTTT GGAGAGGAGG
GAGAAGCACG AGAAGAGACG AGACGTCTCT CCGGAGCGGA GGCGAGAGCG GTCACCCACC
CGCAGGAGAG ACGGCTCCCC CAGCCGGCGG AGACGGTCTC TGGAGAGACT CCTGGAGCAG
AGGAGGTCCC CCGAGCGCAG GAGAGGGGGC TCGCCCGAGC GCAGGGCCAA GTCCACCGAC
CGGAGGCGCG CACGCTCCCC CGAGCGCAGG AGAGAGCGGT CCCTGGACAA AAGGAACAGA
GAGGACAGAG CCAGCCACCG AGAAAGGGAA GAGGCGAATC TGAAACAGGA TGCCGGCAGA
AGTTCCAGAC ATCCCCCGGA GCAGAGAAGG CGACCTTACA AAGAATGTAG CACCGACCTC
AGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001028229.1
CDS527..4915
Misc Feature(1)518..520(+)
Misc Feature(2)569..826(+)
Misc Feature(3)605..799(+)
Misc Feature(4)890..1405(+)
Misc Feature(5)890..898(+)
Misc Feature(6)1433..1522(+)
Misc Feature(7)1475..1510(+)
Misc Feature(8)1610..1702(+)
Misc Feature(9)1652..1687(+)
Misc Feature(10)1952..2155(+)
Misc Feature(11)1967..2146(+)
Misc Feature(12)2447..2686(+)
Misc Feature(13)2480..2647(+)
Misc Feature(14)2981..3208(+)
Misc Feature(15)2990..3169(+)
Misc Feature(16)3428..3712(+)
Misc Feature(17)3464..3679(+)
Misc Feature(18)3890..4123(+)
Misc Feature(19)3920..4099(+)
Exon (1)1..839
Gene:MAGI1
Gene Synonym:
Exon (2)840..956
Gene:MAGI1
Gene Synonym:
Exon (3)957..1076
Gene:MAGI1
Gene Synonym:
Exon (4)1077..1283
Gene:MAGI1
Gene Synonym:
Exon (5)1284..1485
Gene:MAGI1
Gene Synonym:
Exon (6)1486..1568
Gene:MAGI1
Gene Synonym:
Exon (7)1569..1604
Gene:MAGI1
Gene Synonym:
Exon (8)1605..1662
Gene:MAGI1
Gene Synonym:
Exon (9)1663..1796
Gene:MAGI1
Gene Synonym:
Exon (10)1797..1889
Gene:MAGI1
Gene Synonym:
Exon (11)1890..2072
Gene:MAGI1
Gene Synonym:
Exon (12)2073..2693
Gene:MAGI1
Gene Synonym:
Exon (13)2694..2725
Gene:MAGI1
Gene Synonym:
Exon (14)2726..2942
Gene:MAGI1
Gene Synonym:
Exon (15)2943..3034
Gene:MAGI1
Gene Synonym:
Exon (16)3035..3227
Gene:MAGI1
Gene Synonym:
Exon (17)3228..3521
Gene:MAGI1
Gene Synonym:
Exon (18)3522..3722
Gene:MAGI1
Gene Synonym:
Exon (19)3723..3816
Gene:MAGI1
Gene Synonym:
Exon (20)3817..3877
Gene:MAGI1
Gene Synonym:
Exon (21)3878..4021
Gene:MAGI1
Gene Synonym:
Exon (22)4022..4160
Gene:MAGI1
Gene Synonym:
Exon (23)4161..7062
Gene:MAGI1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001033057
Organism Homo sapiens (human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001033057

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGTCCAAAG TGATCCAGAA GAAGAACCAC TGGACTAGCA GGGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GAGGCGCGGA GCACGGGGAG
TTTCCGTACG TCGGAGCGGT GGCGGCGGTC GAGGCAGCGG GGCTTCCCGG CGGCGGCGAG
GGCCCGAGGC TGGGCGAAGG GGAGCTGCTT CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC
TTGCCCCGCT ATGACGTGCT GGGGGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAG
GCCGTCAGAC AAGGAGGAAG GCTGAACAAG GACCTGCGAC ATTTTCTCAA TCAGCGATTC
CAGAAGGGGT CTCCTGATCA TGAGCTCCAG CAGACCATAA GGGATAACCT TTACCGCCAT
GCTGTGCCTT GCACAACCCG ATCTCCCAGA GAAGGAGAAG TGCCTGGCGT GGACTATAAC
TTTCTGACTG TGAAGGAGTT CTTGGACCTC GAGCAGAGTG GGACTCTTCT GGAAGTCGGC
ACCTATGAAG GAAACTATTA TGGGACACCC AAGCCTCCTA GCCAGCCAGT CAGTGGGAAA
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CTAAGCAGTC GACCCCGAAG
CGAACCAAGT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACGCGGA GAATGAGGAG
GAGGATGACG TTCCTGAAAT GAACAGCAGC TTTACAGCCG ATTCTGGTGA ACAAGAGGAG
CACACTCTCC AAGAAACAGC ATTACCACCT GTGAATAGTA GCATCATCGC TGCTCCCATC
ACGGACCCTT CTCAGAAGTT CCCTCAATAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC
CATAACACGA AAACAACATC TTGGTTAGAC CCTCGGTGCC TAAACAAGCA GCAGAAGCCA
CTGGAAGAGT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAA
CTGCCTGCTG GTTGGGAAAA GATTGAAGAC CCTGTCTATG GTATCTACTA TGTAGACCAC
ATCAACAGGA AGACACAATA TGAGAACCCG GTTCTAGAAG CCAAACGGAA GAAGCAGCTT
GAGCAGCAGC AGCAGCAGCA ACAGCAGCAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
CAGACAGAAG AATGGACAGA AGATCACTCA GCCCTTGTGC CTCCTGTTAT TCCAAACCAC
CCTCCAAGCA ATCCAGAGCC AGCCAGAGAA GTTCCACTTC AGGGCAAACC CTTTTTTACA
CGAAACCCTT CTGAGTTGAA AGGCAAGTTC ATTCACACAA AGCTGCGGAA AAGCAGTCGT
GGCTTTGGCT TCACGGTGGT TGGAGGGGAT GAACCTGATG AGTTTCTCCA GATCAAGAGC
TTGGTCCTAG ATGGTCCTGC TGCATTGGAT GGCAAGATGG AAACAGGGGA TGTGATTGTA
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA GATCTTCCAG
TCCATCCCCA TTGGTGCCAG CGTGGACCTT GAACTCTGCC GAGGTTATCC ATTGCCTTTT
GATCCAGATG ACCCCAATAC AAGTTTAGTG ACCTCGGTAG CCATTTTGGA TAAAGAACCA
ATTATTGTGA ATGGGCAAGA GACCTATGAT TCACCAGCTA GCCACAGTAG TAAAACAGGC
AAAGTCAATG GCATGAAGGA TGCCAGGCCA AGCAGCCCAG CGGACGTGGC TTCAAATAGT
TCTCATGGTT ATCCTAATGA CACTGTTTCT TTGGCTTCCT CCATAGCCAC TCAGCCAGAA
CTCATAACTG TTCATATTGT CAAAGGGCCA ATGGGCTTTG GTTTTACTAT CGCAGACAGT
CCTGGTGGGG GTGGCCAAAG AGTGAAACAG ATTGTTGACA GCCCAAGGTG CCGAGGCCTG
AAAGAAGGGG ATCTCATAGT GGAAGTTAAT AAGAAGAACG TGCAGGCCCT AACTCACAAC
CAAGTGGTGG ATATGCTGGT TGAGTGTCCT AAGGGAAGTG AGGTCACATT GTTGGTGCAA
CGAGGAGGGC TGCCAGTTCC CAAGAAGAGC CCAAAGTCGC AACCACTGGA AAGGAAAGAC
AGCCAGAATA GTTCTCAGCA CAGTGTTTCC AGCCACCGAA GCCTGCACAC AGCATCCCCA
AGCCACAGCA CACAGGTGCT CCCCGAGTTC CCACCTGCAG AGGCCCAAGC TCCAGATCAA
ACTGACAGCT CTGGCCAGAA AAAACCAGAT CCTTTTAAAA TCTGGGCCCA GTCCAGGAGC
ATGTATGAAA ACCGACTTCC AGATTACCAG GAACAGGACA TCTTCCTCTG GAGAAAAGAG
ACTGGATTTG GATTTAGGAT TCTGGGTGGA AATGAACCAG GGGAACCTAT TTATATTGGT
CACATCGTAC CACTGGGTGC TGCTGATACT GACGGCCGCC TGAGGTCTGG AGATGAATTA
ATCTGTGTGG ATGGGACGCC AGTAATTGGA AAATCACACC AGCTTGTGGT CCAGCTTATG
CAACAAGCTG CCAAGCAAGG CCACGTCAAT CTCACGGTGC GGCGTAAAGT GGTTTTTGCG
GTGCCCAAAA CCGAGAACGA GGTGCCCTCG CCAGCCTCCT CTCATCACAG TAGCAACCAG
CCGGCCTCGC TGACAGAAGA GAAGCGCACT CCGCAGGGCA GCCAGAACTC GCTGAACACG
GTGAGCTCGG GCAGCGGCAG CACCAGCGGC ATCGGCAGTG GCGGCGGCGG GGGCAGCGGC
GTGGTCAGCA CCGTGGTGCA GCCCTACGAC GTGGAGATCC GGCGCGGGGA GAACGAGGGC
TTCGGCTTCG TCATCGTGTC CTCGGTGAGC AGGCCCGAAG CAGGCACGAC CTTTGGCAAT
GCATGTGTGG CTATGCCTCA CAAAATAGGT CGGATTATTG AGGGGAGCCC TGCTGACCGC
TGTGGCAAGC TGAAAGTAGG AGACCGGATC TTGGCAGTAA ATGGATGTTC CATCACCAAC
AAATCCCATT CAGACATTGT GAACCTAATC AAGGAAGCGG GAAACACAGT TACCCTCCGC
ATCATTCCTG GGGATGAGTC CTCGAATGCC ACCTTGCTGA CCAATGCAGA GAAGATTGCC
ACCATCACCA CCACACACAC CCCTTCTCAG CAAGGGACCC AGGAGACAAG GAATACCACC
AAACCAAAGC AGGAATCTCA ATTTGAGTTC AAAGCACCCC AAGCAACACA GGAGCAAGAT
TTTTACACTG TGGAACTGGA AAGAGGAGCC AAGGGATTTG GCTTTAGCCT TCGAGGAGGC
CGAGAGTATA ACATGGACCT CTATGTTCTG CGCTTAGCAG AGGACGGTCC TGCGGAGAGG
TGTGGAAAGA TGAGGATTGG TGATGAAATT TTAGAGATCA ATGGTGAGAC CACCAAAAAC
ATGAAGCATT CTCGAGCTAT AGAACTGATT AAGAATGGTG GCCGCAGAGT TCGTCTGTTT
CTGAAGCGGG GAGACGGCTC AGTACCAGAA TATGACCCCA GCAGCGACCG CCACGGCCCC
GCCACCGGTC CACAAGGTGT TCCGGAAGTG AGGGCCGGGC CCGACCGCCG GCAGCATCCG
TCATTGGAGT CCAGTTACCC ACCCGATCTT CACAAATCAT CACCACATGG GGAAAAGCGG
GCACACGCAA GGGATCCGAA AGGCAGCAGA GAGTATAGCA GACAACCCAA TGAACACCAC
ACCTGGAATG GGACTTCGAG GAAACCCGAC AGCGGGGCAT GCCGACCCAA GGACCGGGCG
CCGGAGGGAC GGAGGGACGC GCAGGCCGAG CGCGCGGCAG CCGCCAACGG CCCCAAGAGG
CGGTCCCCAG AGAAGCGGAG GGAGGGCACC CGCAGCGCCG ACAACACTTT GGAGAGGAGG
GAGAAGCACG AGAAGAGACG AGACGTCTCT CCGGAGCGGA GGCGAGAGCG GTCACCCACC
CGCAGGAGAG ACGGCTCCCC CAGCCGGCGG AGACGGTCTC TGGAGAGACT CCTGGAGCAG
AGGAGGTCCC CCGAGCGCAG GAGAGGGGGC TCGCCCGAGC GCAGGGCCAA GTCCACCGAC
CGGAGGCGCG CACGCTCCCC CGAGCGCAGG AGAGAGCGGT CCCTGGACAA AAGGAACAGA
GAGGACAGAG CCAGCCACCG AGAAAGGGAA GAGGCGAATC TGAAACAGGA TGCCGGCAGA
AGTTCCAGAC ATCCCCCGGA GCAGAGAAGG CGACCTTACA AAGAATGTAG CACCGACCTC
AGTATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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