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MMP20 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MMP20
Entrez Gene ID 9313
Full Name matrix metallopeptidase 20
Synonyms AI2A2, MMP-20
General protein information
Preferred Names
matrix metalloproteinase-20
Names
matrix metalloproteinase-20
enamel metalloproteinase
matrix metalloproteinase 20 (enamelysin)
NP_004762.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q22.3

Summary Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3. [provided by RefSeq, Aug 2011]. lac of sum
Disorder MIM:

604629

Disorder Html: Amelogenesis imperfecta, type IIA2, 612529 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_004771 NP_004762 matrix metalloproteinase-20 preproprotein



Homo sapiens (human) MMP20 NP_004762.2
Pan troglodytes (chimpanzee) MMP20 XP_001153208.1
Macaca mulatta (Rhesus monkey) MMP20 XP_001097602.1
Canis lupus familiaris (dog) MMP20 XP_854639.1
Bos taurus (cattle) MMP20 NP_776816.1
Mus musculus (house mouse) Mmp20 NP_038931.1
Rattus norvegicus (Norway rat) Mmp20 NP_001100270.1
Danio rerio (zebrafish) LOC100329766 XP_002664668.3
Xenopus (Silurana) tropicalis (western clawed frog) mmp20 XP_002934967.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MMP20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMP20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20263 NM_004771 Homo sapiens matrix metallopeptidase 20 (MMP20), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20263
Clone ID Related Accession (Same CDS sequence) NM_004771
Accession Version NM_004771.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1452bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product matrix metalloproteinase-20 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Y12779.1 and AJ296177.1. This sequence is a reference standard in the RefSeqGene project. On Mar 11, 2004 this sequence version replaced gi:13027805. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y12779.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAAGGTGC TCCCTGCATC TGGCCTTGCT GTCTTCCTCA TCATGGCTTT GAAGTTTTCC 
ACTGCAGCCC CCTCCCTAGT TGCAGCCTCC CCCAGGACCT GGAGGAACAA CTACCGCCTC
GCACAGGCGT ATCTTGACAA ATATTACACA AATAAAGAAG GACACCAGAT TGGTGAGATG
GTTGCAAGAG GAAGCAATTC CATGATAAGG AAGATTAAGG AGCTACAAGC GTTCTTTGGC
CTCCAAGTCA CCGGGAAGTT AGACCAGACC ACAATGAACG TGATCAAGAA GCCTCGCTGT
GGAGTTCCTG ATGTGGCCAA TTATCGCCTC TTCCCTGGTG AACCCAAATG GAAAAAAAAT
ACTTTGACAT ACAGAATATC TAAATACACA CCTTCCATGA GTTCTGTCGA GGTGGACAAA
GCAGTGGAGA TGGCCTTGCA GGCCTGGAGT AGCGCCGTCC CTCTGAGCTT TGTCAGAATA
AACTCAGGAG AAGCGGATAT TATGATATCT TTTGAAAATG GAGATCACGG GGATTCCTAT
CCATTCGATG GGCCTCGGGG GACTCTAGCC CATGCATTTG CTCCTGGAGA AGGCCTGGGA
GGAGATACAC ATTTCGACAA TGCTGAGAAG TGGACTATGG GAACGAATGG TTTTAATTTG
TTTACCGTTG CTGCTCATGA ATTTGGCCAT GCCCTGGGCC TGGCCCATTC CACAGACCCA
TCAGCACTGA TGTACCCAAC TTATAAGTAC AAGAATCCCT ATGGATTCCA CCTCCCCAAA
GATGATGTGA AAGGGATCCA GGCATTATAC GGACCTCGGA AAGTATTCCT GGGGAAGCCC
ACTCTGCCCC ATGCCCCCCA TCACAAGCCA TCCATCCCTG ACCTCTGTGA CTCCAGCTCA
TCCTTTGACG CTGTGACAAT GCTGGGGAAG GAGCTCCTGC TCTTCAAGGA CCGGATTTTC
TGGAGACGGC AGGTTCACTT GCGGACAGGA ATTCGGCCCA GCACTATTAC CAGCTCCTTC
CCCCAGCTCA TGTCCAATGT GGATGCAGCT TACGAAGTGG CTGAGAGGGG CACTGCTTAC
TTCTTCAAAG GTCCCCACTA CTGGATAACA AGAGGATTCC AAATGCAAGG TCCTCCTCGG
ACTATTTATG ACTTTGGATT TCCAAGGCAC GTGCAGCAAA TAGATGCTGC TGTCTACCTC
AGGGAGCCAC AGAAGACCCT TTTCTTTGTG GGAGATGAAT ACTACAGCTA CGACGAAAGG
AAAAGGAAAA TGGAAAAAGA CTATCCAAAG AATACTGAAG AAGAATTTTC AGGAGTAAAT
GGCCAAATCG ATGCTGCTGT AGAATTAAAT GGCTACATTT ACTTCTTTTC AGGACCAAAA
ACATACAAGT ATGACACAGA GAAGGAAGAT GTGGTTAGTG TGGTGAAATC TAGTTCCTGG
ATTGGTTGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004762.2
CDS14..1465
Misc Feature(1)128..298(+)
Misc Feature(2)305..328(+)
Misc Feature(3)359..826(+)
Misc Feature(4)359..826(+)
Misc Feature(5)515..757(+)
Misc Feature(6)689..721(+)
Misc Feature(7)890..1462(+)
Misc Feature(8)890..1042(+)
Misc Feature(9)920..1351(+)
Misc Feature(10)1043..1180(+)
Misc Feature(11)1184..1330(+)
Misc Feature(12)1331..1462(+)
Exon (1)1..139
Gene:MMP20
Gene Synonym:
Exon (2)140..387
Gene:MMP20
Gene Synonym:
Exon (3)388..536
Gene:MMP20
Gene Synonym:
Exon (4)537..662
Gene:MMP20
Gene Synonym:
Exon (5)663..824
Gene:MMP20
Gene Synonym:
Exon (6)825..966
Gene:MMP20
Gene Synonym:
Exon (7)967..1103
Gene:MMP20
Gene Synonym:
Exon (8)1104..1260
Gene:MMP20
Gene Synonym:
Exon (9)1261..1364
Gene:MMP20
Gene Synonym:
Exon (10)1365..1956
Gene:MMP20
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004771
Organism Homo sapiens (human)
Definition Homo sapiens matrix metallopeptidase 20 (MMP20), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004771

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAAGGTGC TCCCTGCATC TGGCCTTGCT GTCTTCCTCA TCATGGCTTT GAAGTTTTCC 
ACTGCAGCCC CCTCCCTAGT TGCAGCCTCC CCCAGGACCT GGAGGAACAA CTACCGCCTC
GCACAGGCGT ATCTTGACAA ATATTACACA AATAAAGAAG GACACCAGAT TGGTGAGATG
GTTGCAAGAG GAAGCAATTC CATGATAAGG AAGATTAAGG AGCTACAAGC GTTCTTTGGC
CTCCAAGTCA CCGGGAAGTT AGACCAGACC ACAATGAACG TGATCAAGAA GCCTCGCTGT
GGAGTTCCTG ATGTGGCCAA TTATCGCCTC TTCCCTGGTG AACCCAAATG GAAAAAAAAT
ACTTTGACAT ACAGAATATC TAAATACACA CCTTCCATGA GTTCTGTCGA GGTGGACAAA
GCAGTGGAGA TGGCCTTGCA GGCCTGGAGT AGCGCCGTCC CTCTGAGCTT TGTCAGAATA
AACTCAGGAG AAGCGGATAT TATGATATCT TTTGAAAATG GAGATCACGG GGATTCCTAT
CCATTCGATG GGCCTCGGGG GACTCTAGCC CATGCATTTG CTCCTGGAGA AGGCCTGGGA
GGAGATACAC ATTTCGACAA TGCTGAGAAG TGGACTATGG GAACGAATGG TTTTAATTTG
TTTACCGTTG CTGCTCATGA ATTTGGCCAT GCCCTGGGCC TGGCCCATTC CACAGACCCA
TCAGCACTGA TGTACCCAAC TTATAAGTAC AAGAATCCCT ATGGATTCCA CCTCCCCAAA
GATGATGTGA AAGGGATCCA GGCATTATAC GGACCTCGGA AAGTATTCCT GGGGAAGCCC
ACTCTGCCCC ATGCCCCCCA TCACAAGCCA TCCATCCCTG ACCTCTGTGA CTCCAGCTCA
TCCTTTGACG CTGTGACAAT GCTGGGGAAG GAGCTCCTGC TCTTCAAGGA CCGGATTTTC
TGGAGACGGC AGGTTCACTT GCGGACAGGA ATTCGGCCCA GCACTATTAC CAGCTCCTTC
CCCCAGCTCA TGTCCAATGT GGATGCAGCT TACGAAGTGG CTGAGAGGGG CACTGCTTAC
TTCTTCAAAG GTCCCCACTA CTGGATAACA AGAGGATTCC AAATGCAAGG TCCTCCTCGG
ACTATTTATG ACTTTGGATT TCCAAGGCAC GTGCAGCAAA TAGATGCTGC TGTCTACCTC
AGGGAGCCAC AGAAGACCCT TTTCTTTGTG GGAGATGAAT ACTACAGCTA CGACGAAAGG
AAAAGGAAAA TGGAAAAAGA CTATCCAAAG AATACTGAAG AAGAATTTTC AGGAGTAAAT
GGCCAAATCG ATGCTGCTGT AGAATTAAAT GGCTACATTT ACTTCTTTTC AGGACCAAAA
ACATACAAGT ATGACACAGA GAAGGAAGAT GTGGTTAGTG TGGTGAAATC TAGTTCCTGG
ATTGGTTGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Identification of the effects of aging-related gene-matrix metalloproteinase on allograft outcomes in kidney transplantation
Transplant. Proc. 45 (6), 2158-2164 (2013)
Han SS, Lee H, Oh YJ, Lee JP, Kim S, Ha J, Kim SJ, Park MH, Kim YS and Kim DK.


book

Homozygous and compound heterozygous MMP20 mutations in amelogenesis imperfecta
J. Dent. Res. 92 (7), 598-603 (2013)
Gasse B, Karayigit E, Mathieu E, Jung S, Garret A, Huckert M, Morkmued S, Schneider C, Vidal L, Hemmerle J, Sire JY and Bloch-Zupan A.


book

Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array
Nat. Genet. 45 (4), 385-391 (2013)
Eeles RA, Olama AA, Benlloch S, Saunders EJ, Leongamornlert DA, Tymrakiewicz M, Ghoussaini M, Luccarini C, Dennis J, Jugurnauth-Little S, Dadaev T, Neal DE, Hamdy FC, Donovan JL, Muir K, Giles GG, Severi G, Wiklund F, Gronberg H, Haiman CA, Schumacher F, Henderson BE, Le Marchand L, Lindstrom S, Kraft P, Hunter DJ, Gapstur S, Chanock SJ, Berndt SI, Albanes D, Andriole G, Schleutker J, Weischer M, Canzian F, Riboli E, Key TJ, Travis RC, Campa D, Ingles SA, John EM, Hayes RB, Pharoah PD, Pashayan N, Khaw KT, Stanford JL, Ostrander EA, Signorello LB, Thibodeau SN, Schaid D, Maier C, Vogel W, Kibel AS, Cybulski C, Lubinski J, Cannon-Albright L, Brenner H, Park JY, Kaneva R, Batra J, Spurdle AB, Clements JA, Teixeira MR, Dicks E, Lee A, Dunning AM, Baynes C, Conroy D, Maranian MJ, Ahmed S, Govindasami K, Guy M, Wilkinson RA, Sawyer EJ, Morgan A, Dearnaley DP, Horwich A, Huddart RA, Khoo VS, Parker CC, Van As NJ, Woodhouse CJ, Thompson A, Dudderidge T, Ogden C, Cooper CS, Lophatananon A, Cox A, Southey MC, Hopper JL, English DR, Aly M, Adolfsson J, Xu J, Zheng SL, Yeager M, Kaaks R, Diver WR, Gaudet MM, Stern MC, Corral R, Joshi AD, Shahabi A, Wahlfors T, Tammela TL, Auvinen A, Virtamo J, Klarskov P, Nordestgaard BG, Roder MA, Nielsen SF, Bojesen SE, Siddiq A, Fitzgerald LM, Kolb S, Kwon EM, Karyadi DM, Blot WJ, Zheng W, Cai Q, McDonnell SK, Rinckleb AE, Drake B, Colditz G, Wokolorczyk D, Stephenson RA, Teerlink C, Muller H, Rothenbacher D, Sellers TA, Lin HY, Slavov C, Mitev V, Lose F, Srinivasan S, Maia S, Paulo P, Lange E, Cooney KA, Antoniou AC, Vincent D, Bacot F, Tessier DC, Kote-Jarai Z and Easton DF.


book

Novel KLK4 and MMP20 mutations discovered by whole-exome sequencing
J. Dent. Res. 92 (3), 266-271 (2013)
Wang SK, Hu Y, Simmer JP, Seymen F, Estrella NM, Pal S, Reid BM, Yildirim M, Bayram M, Bartlett JD and Hu JC.


book

Genetic variation in MMP20 contributes to higher caries experience
J Dent 40 (5), 381-386 (2012)
Tannure PN, Kuchler EC, Lips A, Costa Mde C, Luiz RR, Granjeiro JM and Vieira AR.


book

Matrix metalloproteinases 19 and 20 cleave aggrecan and cartilage oligomeric matrix protein (COMP)
FEBS Lett. 478 (1-2), 52-56 (2000)
Stracke JO, Fosang AJ, Last K, Mercuri FA, Pendas AM, Llano E, Perris R, Di Cesare PE, Murphy G and Knauper V.


book

Matrix metalloproteinases
J. Biol. Chem. 274 (31), 21491-21494 (1999)
Nagase H and Woessner JF Jr.


book

Proteinases in developing dental enamel
Crit. Rev. Oral Biol. Med. 10 (4), 425-441 (1999)
Bartlett JD and Simmer JP.


book

Identification and structural and functional characterization of human enamelysin (MMP-20)
Biochemistry 36 (49), 15101-15108 (1997)
Llano E, Pendas AM, Knauper V, Sorsa T, Salo T, Salido E, Murphy G, Simmer JP, Bartlett JD and Lopez-Otin C.


book

Fine physical mapping of the human matrix metalloproteinase genes clustered on chromosome 11q22.3
Genomics 37 (2), 266-268 (1996)
Pendas AM, Santamaria I, Alvarez MV, Pritchard M and Lopez-Otin C.


 
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