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VAMP3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol VAMP3
Entrez Gene ID 9341
Full Name vesicle-associated membrane protein 3
Synonyms CEB
General protein information
Preferred Names
vesicle-associated membrane protein 3
Names
vesicle-associated membrane protein 3
VAMP-3
cellubrevin
synaptobrevin-3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p36.23

Summary Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

603657


mRNA and Protein(s)

mRNA Protein Name
NM_004781 NP_004772 vesicle-associated membrane protein 3



Homo sapiens (human) VAMP3 NP_004772.1
Pan troglodytes (chimpanzee) VAMP3 NP_001233512.1
Macaca mulatta (Rhesus monkey) VAMP3 XP_001095950.1
Canis lupus familiaris (dog) VAMP3 XP_005620536.1
Bos taurus (cattle) VAMP3 NP_001039380.1
Mus musculus (house mouse) Vamp3 NP_033524.1
Rattus norvegicus (Norway rat) Vamp3 NP_476438.1
Gallus gallus (chicken) VAMP3 NP_001034578.1
Danio rerio (zebrafish) vamp3 NP_001002073.1
Xenopus (Silurana) tropicalis (western clawed frog) vamp3 NP_001016972.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VAMP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VAMP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu15733
NM_004781 Homo sapiens vesicle-associated membrane protein 3 (VAMP3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu15733
Clone ID Related Accession (Same CDS sequence) NM_004781
Accession Version NM_004781.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 303bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product vesicle-associated membrane protein 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005941.1 and BC003570.1. On Feb 13, 2004 this sequence version replaced gi:9257252. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003570.1, BC007050.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGTCTACAG GTCCAACTGC TGCCACTGGC AGTAATCGAA GACTTCAGCA GACACAAAAT 
CAAGTAGATG AGGTGGTGGA CATAATGCGA GTTAACGTGG ACAAGGTTCT GGAAAGAGAC
CAGAAGCTCT CTGAGTTAGA CGACCGTGCA GACGCACTGC AGGCAGGCGC TTCTCAATTT
GAAACGAGCG CAGCCAAGTT GAAGAGGAAA TATTGGTGGA AGAATTGCAA GATGTGGGCA
ATCGGGATTA CTGTTCTGGT TATCTTCATC ATCATCATCA TCGTGTGGGT TGTCTCTTCA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004772.1
CDS116..418
Misc Feature(1)74..76(+)
Misc Feature(2)119..121(+)
Misc Feature(3)146..>358(+)
Misc Feature(4)152..340(+)
Misc Feature(5)155..340(+)
Misc Feature(6)230..232(+)
Misc Feature(7)347..409(+)
Exon (1)1..117
Gene:VAMP3
Gene Synonym:
Exon (2)118..187
Gene:VAMP3
Gene Synonym:
Exon (3)188..346
Gene:VAMP3
Gene Synonym:
Exon (4)347..398
Gene:VAMP3
Gene Synonym:
Exon (5)399..2205
Gene:VAMP3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004781
Organism Homo sapiens (human)
Definition Homo sapiens vesicle-associated membrane protein 3 (VAMP3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004781

ORF Insert Sequence:

1
61
121
181
241
301
ATGTCTACAG GTCCAACTGC TGCCACTGGC AGTAATCGAA GACTTCAGCA GACACAAAAT 
CAAGTAGATG AGGTGGTGGA CATAATGCGA GTTAACGTGG ACAAGGTTCT GGAAAGAGAC
CAGAAGCTCT CTGAGTTAGA CGACCGTGCA GACGCACTGC AGGCAGGCGC TTCTCAATTT
GAAACGAGCG CAGCCAAGTT GAAGAGGAAA TATTGGTGGA AGAATTGCAA GATGTGGGCA
ATCGGGATTA CTGTTCTGGT TATCTTCATC ATCATCATCA TCGTGTGGGT TGTCTCTTCA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

SNAP-23 and VAMP-3 contribute to the release of IL-6 and TNFalpha from a human synovial sarcoma cell line
FEBS J. 281 (3), 750-765 (2014)
Boddul SV, Meng J, Dolly JO and Wang J.


book

Goliath family E3 ligases regulate the recycling endosome pathway via VAMP3 ubiquitylation
EMBO J. 32 (4), 524-537 (2013)
Yamazaki Y, Schonherr C, Varshney GK, Dogru M, Hallberg B and Palmer RH.


book

Regulation of integrin endocytic recycling and chemotactic cell migration by syntaxin 6 and VAMP3 interaction
J. Cell. Sci. 125 (PT 16), 3827-3839 (2012)
Riggs KA, Hasan N, Humphrey D, Raleigh C, Nevitt C, Corbin D and Hu C.


book

Genome-wide association study of periodontal pathogen colonization
J. Dent. Res. 91 (7 SUPPL), 21S-28S (2012)
Divaris K, Monda KL, North KE, Olshan AF, Lange EM, Moss K, Barros SP, Beck JD and Offenbacher S.


book

Genome-wide meta-analysis increases to 71 the number of confirmed Crohn's disease susceptibility loci
Nat. Genet. 42 (12), 1118-1125 (2010)
Franke A, McGovern DP, Barrett JC, Wang K, Radford-Smith GL, Ahmad T, Lees CW, Balschun T, Lee J, Roberts R, Anderson CA, Bis JC, Bumpstead S, Ellinghaus D, Festen EM, Georges M, Green T, Haritunians T, Jostins L, Latiano A, Mathew CG, Montgomery GW, Prescott NJ, Raychaudhuri S, Rotter JI, Schumm P, Sharma Y, Simms LA, Taylor KD, Whiteman D, Wijmenga C, Baldassano RN, Barclay M, Bayless TM, Brand S, Buning C, Cohen A, Colombel JF, Cottone M, Stronati L, Denson T, De Vos M, D'Inca R, Dubinsky M, Edwards C, Florin T, Franchimont D, Gearry R, Glas J, Van Gossum A, Guthery SL, Halfvarson J, Verspaget HW, Hugot JP, Karban A, Laukens D, Lawrance I, Lemann M, Levine A, Libioulle C, Louis E, Mowat C, Newman W, Panes J, Phillips A, Proctor DD, Regueiro M, Russell R, Rutgeerts P, Sanderson J, Sans M, Seibold F, Steinhart AH, Stokkers PC, Torkvist L, Kullak-Ublick G, Wilson D, Walters T, Targan SR, Brant SR, Rioux JD, D'Amato M, Weersma RK, Kugathasan S, Griffiths AM, Mansfield JC, Vermeire S, Duerr RH, Silverberg MS, Satsangi J, Schreiber S, Cho JH, Annese V, Hakonarson H, Daly MJ and Parkes M.


book

Syntaxin 13 mediates cycling of plasma membrane proteins via tubulovesicular recycling endosomes
J. Cell Biol. 143 (4), 957-971 (1998)
Prekeris R, Klumperman J, Chen YA and Scheller RH.


book

Interaction of Munc-18-2 with syntaxin 3 controls the association of apical SNAREs in epithelial cells
J. Cell. Sci. 111 (PT 17), 2681-2688 (1998)
Riento K, Galli T, Jansson S, Ehnholm C, Lehtonen E and Olkkonen VM.


book

A novel tetanus neurotoxin-insensitive vesicle-associated membrane protein in SNARE complexes of the apical plasma membrane of epithelial cells
Mol. Biol. Cell 9 (6), 1437-1448 (1998)
Galli T, Zahraoui A, Vaidyanathan VV, Raposo G, Tian JM, Karin M, Niemann H and Louvard D.


book

Export of cellubrevin from the endoplasmic reticulum is controlled by BAP31
J. Cell Biol. 139 (6), 1397-1410 (1997)
Annaert WG, Becker B, Kistner U, Reth M and Jahn R.


book

Identification of SNAP receptors in rat adipose cell membrane fractions and in SNARE complexes co-immunoprecipitated with epitope-tagged N-ethylmaleimide-sensitive fusion protein
Biochem. J. 320 (PT 2), 429-436 (1996)
Timmers KI, Clark AE, Omatsu-Kanbe M, Whiteheart SW, Bennett MK, Holman GD and Cushman SW.


 
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