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RECQL4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol RECQL4
Entrez Gene ID 9401
Full Name RecQ helicase-like 4
Synonyms RECQ4
General protein information
Preferred Names
ATP-dependent DNA helicase Q4
Names
ATP-dependent DNA helicase Q4
DNA helicase, RecQ-like type 4
DNA helicase, RecQ-like, type 4
NP_004251.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

8

8q24.3

Summary The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. [provided by RefSeq, Jan 2010]. lac of sum
Disorder MIM:

603780

Disorder Html: Rothmund-Thomson syndrome, 268400 (3); RAPADILINO syndrome, 266280

mRNA and Protein(s)

mRNA Protein Name
NM_004260 NP_004251 ATP-dependent DNA helicase Q4
XM_011517380 XP_011515682 ATP-dependent DNA helicase Q4 isoform X1
XM_011517381 XP_011515683 ATP-dependent DNA helicase Q4 isoform X2
XM_011517382 XP_011515684 ATP-dependent DNA helicase Q4 isoform X3
XM_011517383 XP_011515685 ATP-dependent DNA helicase Q4 isoform X4
XM_011517384 XP_011515686 ATP-dependent DNA helicase Q4 isoform X5
XM_011517385 XP_011515687 ATP-dependent DNA helicase Q4 isoform X6


Homo sapiens (human) RECQL4 NP_004251.3
Pan troglodytes (chimpanzee) RECQL4 XP_520023.2
Canis lupus familiaris (dog) RECQL4 XP_005628375.1
Bos taurus (cattle) RECQL4 NP_001091506.1
Mus musculus (house mouse) Recql4 NP_478121.2
Rattus norvegicus (Norway rat) Recql4 NP_001123966.1
Danio rerio (zebrafish) LOC567383 XP_001920631.4
Xenopus (Silurana) tropicalis (western clawed frog) recql4 NP_001072882.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RECQL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RECQL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18758 NM_004260 Homo sapiens RecQ helicase-like 4 (RECQL4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $853.30
$1219.00
OHu62799 XM_011517380 PREDICTED: Homo sapiens RecQ protein-like 4 (RECQL4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu62800 XM_011517381 PREDICTED: Homo sapiens RecQ protein-like 4 (RECQL4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu62801 XM_011517382 PREDICTED: Homo sapiens RecQ protein-like 4 (RECQL4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu62802 XM_011517383 PREDICTED: Homo sapiens RecQ protein-like 4 (RECQL4), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu62803 XM_011517384 PREDICTED: Homo sapiens RecQ protein-like 4 (RECQL4), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu62804 XM_011517385 PREDICTED: Homo sapiens RecQ protein-like 4 (RECQL4), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18758
Clone ID Related Accession (Same CDS sequence) NM_004260
Accession Version NM_004260.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-AUG-2015
Organism Homo sapiens (human)
Product ATP-dependent DNA helicase Q4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN286057.1, AB006532.1 and BC013277.2. This sequence is a reference standard in the RefSeqGene project. On Jan 16, 2010 this sequence version replaced gi:116812615. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB006532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGCCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGTACA GCGAGCCTTC
ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC ACGGTGGCCT TTGGGATGGG GCTGGACCGG
CCAGATGTGC GGGCTGTGCT GCATCTGGGG CTGCCCCCAA GCTTCGAGAG CTACGTGCAG
GCCGTGGGCC GGGCCGGGCG TGACGGGCAG CCTGCCCACT GCCACCTCTT CCTGCAGCCC
CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA CATGTGCACG CCGACAGCAC GGACTTCCTG
GCTGTGAAGA GGCTGGTACA GCGCGTGTTC CCAGCCTGCA CCTGCACCTG CACCAGGCCG
CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA
GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC CCAGGACCCA GAAGGGTCTG CATGGGCCAT
GAGCGGGCAC TCCCAATACA GCTTACCGTA CAGGCTTTGG ACATGCCGGA GGAGGCCATC
GAGACTTTGC TGTGCTACCT GGAGCTGCAC CCACACCACT GGCTGGAGCT GCTGGCGACC
ACCTATACCC ATTGCCGTCT GAACTGCCCT GGGGGCCCTG CCCAGCTCCA GGCCCTGGCC
CACAGGTGTC CCCCTTTGGC TGTGTGCTTG GCCCAGCAGC TGCCTGAGGA CCCAGGGCAA
GGCAGCAGCT CCGTGGAGTT TGACATGGTC AAGCTGGTGG ACTCCATGGG CTGGGAGCTG
GCCTCTGTGC GGCGGGCTCT CTGCCAGCTG CAGTGGGACC ACGAGCCCAG GACAGGTGTG
CGGCGTGGGA CAGGGGTGCT TGTGGAGTTC AGTGAGCTGG CCTTCCACCT TCGCAGCCCG
GGGGACCTGA CCGCTGAGGA GAAGGACCAG ATATGTGACT TCCTCTATGG CCGTGTGCAG
GCCCGGGAGC GCCAGGCCCT GGCCCGTCTG CGCAGAACCT TCCAGGCCTT TCACAGCGTA
GCCTTCCCCA GCTGCGGGCC CTGCCTGGAG CAGCAGGATG AGGAGCGCAG CACCAGGCTC
AAGGACCTGC TCGGCCGCTA CTTTGAGGAA GAGGAAGGGC AGGAGCCGGG AGGCATGGAG
GACGCACAGG GCCCCGAGCC AGGGCAGGCC AGACTCCAGG ATTGGGAGGA CCAGGTCCGC
TGCGACATCC GCCAGTTCCT GTCCCTGAGG CCAGAGGAGA AGTTCTCCAG CAGGGCTGTG
GCCCGCATCT TCCACGGCAT CGGAAGCCCC TGCTACCCGG CCCAGGTGTA CGGGCAGGAC
CGACGCTTCT GGAGAAAATA CCTGCACCTG AGCTTCCATG CCCTGGTGGG CCTGGCCACG
GAAGAGCTCC TGCAGGTGGC CCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004251.3
CDS43..3669
Misc Feature(1)1459..2010(+)
Misc Feature(2)1531..1971(+)
Misc Feature(3)1555..1569(+)
Misc Feature(4)1855..1866(+)
Misc Feature(5)1855..1866(+)
Misc Feature(6)2095..2493(+)
Misc Feature(7)2164..2361(+)
Misc Feature(8)2377..2463(+)
Exon (1)1..126
Gene:RECQL4
Gene Synonym:
Exon (2)127..160
Gene:RECQL4
Gene Synonym:
Exon (3)161..255
Gene:RECQL4
Gene Synonym:
Exon (4)256..396
Gene:RECQL4
Gene Synonym:
Exon (5)397..1173
Gene:RECQL4
Gene Synonym:
Exon (6)1174..1300
Gene:RECQL4
Gene Synonym:
Exon (7)1301..1432
Gene:RECQL4
Gene Synonym:
Exon (8)1433..1525
Gene:RECQL4
Gene Synonym:
Exon (9)1526..1662
Gene:RECQL4
Gene Synonym:
Exon (10)1663..1746
Gene:RECQL4
Gene Synonym:
Exon (11)1747..1920
Gene:RECQL4
Gene Synonym:
Exon (12)1921..2100
Gene:RECQL4
Gene Synonym:
Exon (13)2101..2242
Gene:RECQL4
Gene Synonym:
Exon (14)2243..2505
Gene:RECQL4
Gene Synonym:
Exon (15)2506..2797
Gene:RECQL4
Gene Synonym:
Exon (16)2798..2927
Gene:RECQL4
Gene Synonym:
Exon (17)2928..3097
Gene:RECQL4
Gene Synonym:
Exon (18)3098..3278
Gene:RECQL4
Gene Synonym:
Exon (19)3279..3435
Gene:RECQL4
Gene Synonym:
Exon (20)3436..3544
Gene:RECQL4
Gene Synonym:
Exon (21)3545..3816
Gene:RECQL4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004260
Organism Homo sapiens (human)
Definition Homo sapiens RecQ helicase-like 4 (RECQL4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004260

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGCCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGTACA GCGAGCCTTC
ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC ACGGTGGCCT TTGGGATGGG GCTGGACCGG
CCAGATGTGC GGGCTGTGCT GCATCTGGGG CTGCCCCCAA GCTTCGAGAG CTACGTGCAG
GCCGTGGGCC GGGCCGGGCG TGACGGGCAG CCTGCCCACT GCCACCTCTT CCTGCAGCCC
CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA CATGTGCACG CCGACAGCAC GGACTTCCTG
GCTGTGAAGA GGCTGGTACA GCGCGTGTTC CCAGCCTGCA CCTGCACCTG CACCAGGCCG
CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA
GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC CCAGGACCCA GAAGGGTCTG CATGGGCCAT
GAGCGGGCAC TCCCAATACA GCTTACCGTA CAGGCTTTGG ACATGCCGGA GGAGGCCATC
GAGACTTTGC TGTGCTACCT GGAGCTGCAC CCACACCACT GGCTGGAGCT GCTGGCGACC
ACCTATACCC ATTGCCGTCT GAACTGCCCT GGGGGCCCTG CCCAGCTCCA GGCCCTGGCC
CACAGGTGTC CCCCTTTGGC TGTGTGCTTG GCCCAGCAGC TGCCTGAGGA CCCAGGGCAA
GGCAGCAGCT CCGTGGAGTT TGACATGGTC AAGCTGGTGG ACTCCATGGG CTGGGAGCTG
GCCTCTGTGC GGCGGGCTCT CTGCCAGCTG CAGTGGGACC ACGAGCCCAG GACAGGTGTG
CGGCGTGGGA CAGGGGTGCT TGTGGAGTTC AGTGAGCTGG CCTTCCACCT TCGCAGCCCG
GGGGACCTGA CCGCTGAGGA GAAGGACCAG ATATGTGACT TCCTCTATGG CCGTGTGCAG
GCCCGGGAGC GCCAGGCCCT GGCCCGTCTG CGCAGAACCT TCCAGGCCTT TCACAGCGTA
GCCTTCCCCA GCTGCGGGCC CTGCCTGGAG CAGCAGGATG AGGAGCGCAG CACCAGGCTC
AAGGACCTGC TCGGCCGCTA CTTTGAGGAA GAGGAAGGGC AGGAGCCGGG AGGCATGGAG
GACGCACAGG GCCCCGAGCC AGGGCAGGCC AGACTCCAGG ATTGGGAGGA CCAGGTCCGC
TGCGACATCC GCCAGTTCCT GTCCCTGAGG CCAGAGGAGA AGTTCTCCAG CAGGGCTGTG
GCCCGCATCT TCCACGGCAT CGGAAGCCCC TGCTACCCGG CCCAGGTGTA CGGGCAGGAC
CGACGCTTCT GGAGAAAATA CCTGCACCTG AGCTTCCATG CCCTGGTGGG CCTGGCCACG
GAAGAGCTCC TGCAGGTGGC CCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62799
Clone ID Related Accession (Same CDS sequence) XM_011517380
Accession Version XM_011517380.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent DNA helicase Q4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008046.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGTACA GCGAGCCTTC
ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC ACGGTGGCCT TTGGGATGGG GCTGGACCGG
CCAGATGTGC GGGCTGTGCT GCATCTGGGG CTGCCCCCAA GCTTCGAGAG CTACGTGCAG
GCCGTGGGCC GGGCCGGGCG TGACGGGCAG CCTGCCCACT GCCACCTCTT CCTGCAGCCC
CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA CATGTGCACG CCGACAGCAC GGACTTCCTG
GCTGTGAAGA GGCTGGTACA GCGCGTGTTC CCAGCCTGCA CCTGCACCTG CACCAGGCCG
CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA
GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC CCAGGACCCA GAAGGGTCTG CATGGGCCAT
GAGCGGGCAC TCCCAATACA GCTTACCGTA CAGGCTTTGG ACATGCCGGA GGAGGGTGAG
GAACCTGGGG TAAGCCACAG GGGTGTGGAG GGGCTGTCCC CGCGTCCGCT GAGCCCTGCT
CTGCCCCCAG CCATCGAGAC TTTGCTGTGC TACCTGGAGC TGCACCCACA CCACTGGCTG
GAGCTGCTGG CGACCACCTA TACCCATTGC CGTCTGAACT GCCCTGGGGG CCCTGCCCAG
CTCCAGGCCC TGGCCCACAG GTGTCCCCCT TTGGCTGTGT GCTTGGCCCA GCAGCTGCCT
GAGGACCCAG GGCAAGGCAG CAGCTCCGTG GAGTTTGACA TGGTCAAGCT GGTGGACTCC
ATGGGCTGGG AGCTGGCCTC TGTGCGGCGG GCTCTCTGCC AGCTGCAGTG GGACCACGAG
CCCAGGACAG GTGTGCGGCG TGGGACAGGG GTGCTTGTGG AGTTCAGTGA GCTGGCCTTC
CACCTTCGCA GCCCGGGGGA CTTGACCGCT GAGGAGAAGG ACCAGATATG TGACTTCCTC
TATGGCCGTG TGCAGGCCCG GGAGCGCCAG GCCCTGGCCC GTCTGCGCAG AACCTTCCAG
GCCTTTCACA GCGTAGCCTT CCCCAGCTGC GGGCCCTGCC TGGAGCAGCA GGATGAGGAG
CGCAGCACCA GGCTCAAGGA CCTGCTCGGC CGCTACTTTG AGGAAGAGGA AGGGCAGGAG
CCGGGAGGCA TGGAGGACGC ACAGGGCCCC GAGCCAGGGC AGGCCAGACT CCAGGATTGG
GAGGACCAGG TCCGCTGCGA CATCCGCCAG TTCCTGTCCC TGAGGCCAGA GGAGAAGTTC
TCCAGCAGGG CTGTGGCCCG CATCTTCCAC GGCATCGGAA GCCCCTGCTA CCCGGCCCAG
GTGTACGGGC AGGACCGACG CTTCTGGAGA AAATACCTGC ACCTGAGCTT CCATGCCCTG
GTGGGCCTGG CCACGGAAGA GCTCCTGCAG GTGGCCCGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515682.1
CDS38..3739
Misc Feature(1)1454..2005(+)
Misc Feature(2)1526..1966(+)
Misc Feature(3)1550..1564(+)
Misc Feature(4)1850..1861(+)
Misc Feature(5)2090..2488(+)
Misc Feature(6)2159..2356(+)
Misc Feature(7)2372..2458(+)
Translation

Target ORF information:

RefSeq Version XM_011517380
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens RecQ protein-like 4 (RECQL4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517380

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGTACA GCGAGCCTTC
ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC ACGGTGGCCT TTGGGATGGG GCTGGACCGG
CCAGATGTGC GGGCTGTGCT GCATCTGGGG CTGCCCCCAA GCTTCGAGAG CTACGTGCAG
GCCGTGGGCC GGGCCGGGCG TGACGGGCAG CCTGCCCACT GCCACCTCTT CCTGCAGCCC
CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA CATGTGCACG CCGACAGCAC GGACTTCCTG
GCTGTGAAGA GGCTGGTACA GCGCGTGTTC CCAGCCTGCA CCTGCACCTG CACCAGGCCG
CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA
GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC CCAGGACCCA GAAGGGTCTG CATGGGCCAT
GAGCGGGCAC TCCCAATACA GCTTACCGTA CAGGCTTTGG ACATGCCGGA GGAGGGTGAG
GAACCTGGGG TAAGCCACAG GGGTGTGGAG GGGCTGTCCC CGCGTCCGCT GAGCCCTGCT
CTGCCCCCAG CCATCGAGAC TTTGCTGTGC TACCTGGAGC TGCACCCACA CCACTGGCTG
GAGCTGCTGG CGACCACCTA TACCCATTGC CGTCTGAACT GCCCTGGGGG CCCTGCCCAG
CTCCAGGCCC TGGCCCACAG GTGTCCCCCT TTGGCTGTGT GCTTGGCCCA GCAGCTGCCT
GAGGACCCAG GGCAAGGCAG CAGCTCCGTG GAGTTTGACA TGGTCAAGCT GGTGGACTCC
ATGGGCTGGG AGCTGGCCTC TGTGCGGCGG GCTCTCTGCC AGCTGCAGTG GGACCACGAG
CCCAGGACAG GTGTGCGGCG TGGGACAGGG GTGCTTGTGG AGTTCAGTGA GCTGGCCTTC
CACCTTCGCA GCCCGGGGGA CTTGACCGCT GAGGAGAAGG ACCAGATATG TGACTTCCTC
TATGGCCGTG TGCAGGCCCG GGAGCGCCAG GCCCTGGCCC GTCTGCGCAG AACCTTCCAG
GCCTTTCACA GCGTAGCCTT CCCCAGCTGC GGGCCCTGCC TGGAGCAGCA GGATGAGGAG
CGCAGCACCA GGCTCAAGGA CCTGCTCGGC CGCTACTTTG AGGAAGAGGA AGGGCAGGAG
CCGGGAGGCA TGGAGGACGC ACAGGGCCCC GAGCCAGGGC AGGCCAGACT CCAGGATTGG
GAGGACCAGG TCCGCTGCGA CATCCGCCAG TTCCTGTCCC TGAGGCCAGA GGAGAAGTTC
TCCAGCAGGG CTGTGGCCCG CATCTTCCAC GGCATCGGAA GCCCCTGCTA CCCGGCCCAG
GTGTACGGGC AGGACCGACG CTTCTGGAGA AAATACCTGC ACCTGAGCTT CCATGCCCTG
GTGGGCCTGG CCACGGAAGA GCTCCTGCAG GTGGCCCGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62800
Clone ID Related Accession (Same CDS sequence) XM_011517381
Accession Version XM_011517381.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent DNA helicase Q4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008046.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGGGCAAT
TACGTACGGC TCAACATGAA GCAGAAACAC TACGTGCGGG GCCGGGCACT CCGTAGCAGG
CTCCTCCGCA AGCAGGCATG GAAGCAGAAG TGGCGGAAGA AAGGGGAGTG TTTTGGGGGT
GGTGGTGCCA CAGTCACAAC CAAGGAGTCT TGTTTCCTGA ACGAGCAGTT CGATCACTGG
GCAGCCCAGT GTCCCCGGCC AGCAAGTGAG GAAGACACAG ATGCTGTTGG GCCTGAGCCA
CTGGTTCCTT CACCACAACC TGTACCTGAG GTGCCCAGCC TGGACCCCAC CGTGCTGCCA
CTCTACTCCC TGGGGCCCTC AGGGCAGTTG GCAGAGACGC CGGCTGAGGT GTTCCAGGCC
CTGGAGCAGC TGGGGCACCA AGCCTTTCGC CCTGGGCAGG AGCGTGCAGT CATGCGGATC
CTGTCTGGCA TCTCCACGCT GCTGGTGCTG CCTACAGGTG CCGGCAAGTC CCTGTGCTAC
CAGCTCCCAG CGCTGCTCTA CAGCCGGCGC AGCCCCTGCC TCACGTTGGT CGTCTCTCCC
CTGCTGTCAC TCATGGATGA CCAGGTGTCT GGCCTGCCAC CGTGTCTCAA GGCGGCCTGC
ATACACTCGG GCATGACCAG GAAGCAACGG GAATCTGTCC TGCAGAAGAT TCGGGCAGCC
CAGGTACACG TGCTGATGCT GACACCTGAG GCACTGGTGG GGGCGGGAGG CCTCCCTCCA
GCCGCACAGC TGCCTCCAGT TGCTTTTGCC TGCATTGATG AGGCCCACTG CCTCTCCCAG
TGGTCCCACA ACTTCCGGCC CTGCTACCTG CGCGTCTGCA AGGTGCTTCG GGAGCGCATG
GGCGTGCACT GCTTCCTGGG CCTCACAGCC ACAGCCACAC GCCGCACTGC CAGTGACGTG
GCACAGCACC TGGCTGTGGC TGAAGAGCCT GACCTCCACG GGCCAGCCCC AGTTCCCACC
AACCTGCACC TTTCCGTGTC CATGGACAGG GACACAGACC AGGCACTGTT GACGCTGCTG
CAAGGCAAAC GTTTTCAAAA CCTCGATTCC ATTATCATTT ACTGCAACCG GCGCGAGGAC
ACAGAGCGGA TCGCTGCGCT CCTCCGAACC TGCCTGCACG CAGCCTGGGT CCCAGGGTCT
GGAGGTCGTG CCCCCAAAAC CACAGCCGAG GCCTACCACG CGGGCATGTG CAGCCGGGAA
CGGCGGCGGG TACAGCGAGC CTTCATGCAG GGCCAGTTGC GGGTGGTGGT GGCCACGGTG
GCCTTTGGGA TGGGGCTGGA CCGGCCAGAT GTGCGGGCTG TGCTGCATCT GGGGCTGCCC
CCAAGCTTCG AGAGCTACGT GCAGGCCGTG GGCCGGGCCG GGCGTGACGG GCAGCCTGCC
CACTGCCACC TCTTCCTGCA GCCCCAGGGC GAAGACCTGC GAGAGCTGCG CAGACATGTG
CACGCCGACA GCACGGACTT CCTGGCTGTG AAGAGGCTGG TACAGCGCGT GTTCCCAGCC
TGCACCTGCA CCTGCACCAG GCCGCCCTCG GAGCAGGAAG GGGCCGTGGG TGGGGAGAGG
CCTGTGCCCA AGTACCCCCC TCAAGAGGCT GAGCAGCTTA GCCACCAAGC AGCCCCAGGA
CCCAGAAGGG TCTGCATGGG CCATGAGCGG GCACTCCCAA TACAGCTTAC CGTACAGGCT
TTGGACATGC CGGAGGAGGG TGAGGAACCT GGGGTAAGCC ACAGGGGTGT GGAGGGGCTG
TCCCCGCGTC CGCTGAGCCC TGCTCTGCCC CCAGCCATCG AGACTTTGCT GTGCTACCTG
GAGCTGCACC CACACCACTG GCTGGAGCTG CTGGCGACCA CCTATACCCA TTGCCGTCTG
AACTGCCCTG GGGGCCCTGC CCAGCTCCAG GCCCTGGCCC ACAGGTGTCC CCCTTTGGCT
GTGTGCTTGG CCCAGCAGCT GCCTGAGGAC CCAGGGCAAG GCAGCAGCTC CGTGGAGTTT
GACATGGTCA AGCTGGTGGA CTCCATGGGC TGGGAGCTGG CCTCTGTGCG GCGGGCTCTC
TGCCAGCTGC AGTGGGACCA CGAGCCCAGG ACAGGTGTGC GGCGTGGGAC AGGGGTGCTT
GTGGAGTTCA GTGAGCTGGC CTTCCACCTT CGCAGCCCGG GGGACTTGAC CGCTGAGGAG
AAGGACCAGA TATGTGACTT CCTCTATGGC CGTGTGCAGG CCCGGGAGCG CCAGGCCCTG
GCCCGTCTGC GCAGAACCTT CCAGGCCTTT CACAGCGTAG CCTTCCCCAG CTGCGGGCCC
TGCCTGGAGC AGCAGGATGA GGAGCGCAGC ACCAGGCTCA AGGACCTGCT CGGCCGCTAC
TTTGAGGAAG AGGAAGGGCA GGAGCCGGGA GGCATGGAGG ACGCACAGGG CCCCGAGCCA
GGGCAGGCCA GACTCCAGGA TTGGGAGGAC CAGGTCCGCT GCGACATCCG CCAGTTCCTG
TCCCTGAGGC CAGAGGAGAA GTTCTCCAGC AGGGCTGTGG CCCGCATCTT CCACGGCATC
GGAAGCCCCT GCTACCCGGC CCAGGTGTAC GGGCAGGACC GACGCTTCTG GAGAAAATAC
CTGCACCTGA GCTTCCATGC CCTGGTGGGC CTGGCCACGG AAGAGCTCCT GCAGGTGGCC
CGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515683.1
CDS38..3643
Misc Feature(1)1358..1909(+)
Misc Feature(2)1430..1870(+)
Misc Feature(3)1454..1468(+)
Misc Feature(4)1754..1765(+)
Misc Feature(5)1994..2392(+)
Misc Feature(6)2063..2260(+)
Misc Feature(7)2276..2362(+)
Translation

Target ORF information:

RefSeq Version XM_011517381
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens RecQ protein-like 4 (RECQL4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517381

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGGGCAAT
TACGTACGGC TCAACATGAA GCAGAAACAC TACGTGCGGG GCCGGGCACT CCGTAGCAGG
CTCCTCCGCA AGCAGGCATG GAAGCAGAAG TGGCGGAAGA AAGGGGAGTG TTTTGGGGGT
GGTGGTGCCA CAGTCACAAC CAAGGAGTCT TGTTTCCTGA ACGAGCAGTT CGATCACTGG
GCAGCCCAGT GTCCCCGGCC AGCAAGTGAG GAAGACACAG ATGCTGTTGG GCCTGAGCCA
CTGGTTCCTT CACCACAACC TGTACCTGAG GTGCCCAGCC TGGACCCCAC CGTGCTGCCA
CTCTACTCCC TGGGGCCCTC AGGGCAGTTG GCAGAGACGC CGGCTGAGGT GTTCCAGGCC
CTGGAGCAGC TGGGGCACCA AGCCTTTCGC CCTGGGCAGG AGCGTGCAGT CATGCGGATC
CTGTCTGGCA TCTCCACGCT GCTGGTGCTG CCTACAGGTG CCGGCAAGTC CCTGTGCTAC
CAGCTCCCAG CGCTGCTCTA CAGCCGGCGC AGCCCCTGCC TCACGTTGGT CGTCTCTCCC
CTGCTGTCAC TCATGGATGA CCAGGTGTCT GGCCTGCCAC CGTGTCTCAA GGCGGCCTGC
ATACACTCGG GCATGACCAG GAAGCAACGG GAATCTGTCC TGCAGAAGAT TCGGGCAGCC
CAGGTACACG TGCTGATGCT GACACCTGAG GCACTGGTGG GGGCGGGAGG CCTCCCTCCA
GCCGCACAGC TGCCTCCAGT TGCTTTTGCC TGCATTGATG AGGCCCACTG CCTCTCCCAG
TGGTCCCACA ACTTCCGGCC CTGCTACCTG CGCGTCTGCA AGGTGCTTCG GGAGCGCATG
GGCGTGCACT GCTTCCTGGG CCTCACAGCC ACAGCCACAC GCCGCACTGC CAGTGACGTG
GCACAGCACC TGGCTGTGGC TGAAGAGCCT GACCTCCACG GGCCAGCCCC AGTTCCCACC
AACCTGCACC TTTCCGTGTC CATGGACAGG GACACAGACC AGGCACTGTT GACGCTGCTG
CAAGGCAAAC GTTTTCAAAA CCTCGATTCC ATTATCATTT ACTGCAACCG GCGCGAGGAC
ACAGAGCGGA TCGCTGCGCT CCTCCGAACC TGCCTGCACG CAGCCTGGGT CCCAGGGTCT
GGAGGTCGTG CCCCCAAAAC CACAGCCGAG GCCTACCACG CGGGCATGTG CAGCCGGGAA
CGGCGGCGGG TACAGCGAGC CTTCATGCAG GGCCAGTTGC GGGTGGTGGT GGCCACGGTG
GCCTTTGGGA TGGGGCTGGA CCGGCCAGAT GTGCGGGCTG TGCTGCATCT GGGGCTGCCC
CCAAGCTTCG AGAGCTACGT GCAGGCCGTG GGCCGGGCCG GGCGTGACGG GCAGCCTGCC
CACTGCCACC TCTTCCTGCA GCCCCAGGGC GAAGACCTGC GAGAGCTGCG CAGACATGTG
CACGCCGACA GCACGGACTT CCTGGCTGTG AAGAGGCTGG TACAGCGCGT GTTCCCAGCC
TGCACCTGCA CCTGCACCAG GCCGCCCTCG GAGCAGGAAG GGGCCGTGGG TGGGGAGAGG
CCTGTGCCCA AGTACCCCCC TCAAGAGGCT GAGCAGCTTA GCCACCAAGC AGCCCCAGGA
CCCAGAAGGG TCTGCATGGG CCATGAGCGG GCACTCCCAA TACAGCTTAC CGTACAGGCT
TTGGACATGC CGGAGGAGGG TGAGGAACCT GGGGTAAGCC ACAGGGGTGT GGAGGGGCTG
TCCCCGCGTC CGCTGAGCCC TGCTCTGCCC CCAGCCATCG AGACTTTGCT GTGCTACCTG
GAGCTGCACC CACACCACTG GCTGGAGCTG CTGGCGACCA CCTATACCCA TTGCCGTCTG
AACTGCCCTG GGGGCCCTGC CCAGCTCCAG GCCCTGGCCC ACAGGTGTCC CCCTTTGGCT
GTGTGCTTGG CCCAGCAGCT GCCTGAGGAC CCAGGGCAAG GCAGCAGCTC CGTGGAGTTT
GACATGGTCA AGCTGGTGGA CTCCATGGGC TGGGAGCTGG CCTCTGTGCG GCGGGCTCTC
TGCCAGCTGC AGTGGGACCA CGAGCCCAGG ACAGGTGTGC GGCGTGGGAC AGGGGTGCTT
GTGGAGTTCA GTGAGCTGGC CTTCCACCTT CGCAGCCCGG GGGACTTGAC CGCTGAGGAG
AAGGACCAGA TATGTGACTT CCTCTATGGC CGTGTGCAGG CCCGGGAGCG CCAGGCCCTG
GCCCGTCTGC GCAGAACCTT CCAGGCCTTT CACAGCGTAG CCTTCCCCAG CTGCGGGCCC
TGCCTGGAGC AGCAGGATGA GGAGCGCAGC ACCAGGCTCA AGGACCTGCT CGGCCGCTAC
TTTGAGGAAG AGGAAGGGCA GGAGCCGGGA GGCATGGAGG ACGCACAGGG CCCCGAGCCA
GGGCAGGCCA GACTCCAGGA TTGGGAGGAC CAGGTCCGCT GCGACATCCG CCAGTTCCTG
TCCCTGAGGC CAGAGGAGAA GTTCTCCAGC AGGGCTGTGG CCCGCATCTT CCACGGCATC
GGAAGCCCCT GCTACCCGGC CCAGGTGTAC GGGCAGGACC GACGCTTCTG GAGAAAATAC
CTGCACCTGA GCTTCCATGC CCTGGTGGGC CTGGCCACGG AAGAGCTCCT GCAGGTGGCC
CGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62801
Clone ID Related Accession (Same CDS sequence) XM_011517382
Accession Version XM_011517382.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent DNA helicase Q4 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008046.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGCCCCA GGGCGAAGAC
CTGCGAGAGC TGCGCAGACA TGTGCACGCC GACAGCACGG ACTTCCTGGC TGTGAAGAGG
CTGGTACAGC GCGTGTTCCC AGCCTGCACC TGCACCTGCA CCAGGCCGCC CTCGGAGCAG
GAAGGGGCCG TGGGTGGGGA GAGGCCTGTG CCCAAGTACC CCCCTCAAGA GGCTGAGCAG
CTTAGCCACC AAGCAGCCCC AGGACCCAGA AGGGTCTGCA TGGGCCATGA GCGGGCACTC
CCAATACAGC TTACCGTACA GGCTTTGGAC ATGCCGGAGG AGGGTGAGGA ACCTGGGGTA
AGCCACAGGG GTGTGGAGGG GCTGTCCCCG CGTCCGCTGA GCCCTGCTCT GCCCCCAGCC
ATCGAGACTT TGCTGTGCTA CCTGGAGCTG CACCCACACC ACTGGCTGGA GCTGCTGGCG
ACCACCTATA CCCATTGCCG TCTGAACTGC CCTGGGGGCC CTGCCCAGCT CCAGGCCCTG
GCCCACAGGT GTCCCCCTTT GGCTGTGTGC TTGGCCCAGC AGCTGCCTGA GGACCCAGGG
CAAGGCAGCA GCTCCGTGGA GTTTGACATG GTCAAGCTGG TGGACTCCAT GGGCTGGGAG
CTGGCCTCTG TGCGGCGGGC TCTCTGCCAG CTGCAGTGGG ACCACGAGCC CAGGACAGGT
GTGCGGCGTG GGACAGGGGT GCTTGTGGAG TTCAGTGAGC TGGCCTTCCA CCTTCGCAGC
CCGGGGGACT TGACCGCTGA GGAGAAGGAC CAGATATGTG ACTTCCTCTA TGGCCGTGTG
CAGGCCCGGG AGCGCCAGGC CCTGGCCCGT CTGCGCAGAA CCTTCCAGGC CTTTCACAGC
GTAGCCTTCC CCAGCTGCGG GCCCTGCCTG GAGCAGCAGG ATGAGGAGCG CAGCACCAGG
CTCAAGGACC TGCTCGGCCG CTACTTTGAG GAAGAGGAAG GGCAGGAGCC GGGAGGCATG
GAGGACGCAC AGGGCCCCGA GCCAGGGCAG GCCAGACTCC AGGATTGGGA GGACCAGGTC
CGCTGCGACA TCCGCCAGTT CCTGTCCCTG AGGCCAGAGG AGAAGTTCTC CAGCAGGGCT
GTGGCCCGCA TCTTCCACGG CATCGGAAGC CCCTGCTACC CGGCCCAGGT GTACGGGCAG
GACCGACGCT TCTGGAGAAA ATACCTGCAC CTGAGCTTCC ATGCCCTGGT GGGCCTGGCC
ACGGAAGAGC TCCTGCAGGT GGCCCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515684.1
CDS38..3547
Misc Feature(1)1454..2005(+)
Misc Feature(2)1526..1966(+)
Misc Feature(3)1550..1564(+)
Misc Feature(4)1850..1861(+)
Translation

Target ORF information:

RefSeq Version XM_011517382
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens RecQ protein-like 4 (RECQL4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517382

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGCCCCA GGGCGAAGAC
CTGCGAGAGC TGCGCAGACA TGTGCACGCC GACAGCACGG ACTTCCTGGC TGTGAAGAGG
CTGGTACAGC GCGTGTTCCC AGCCTGCACC TGCACCTGCA CCAGGCCGCC CTCGGAGCAG
GAAGGGGCCG TGGGTGGGGA GAGGCCTGTG CCCAAGTACC CCCCTCAAGA GGCTGAGCAG
CTTAGCCACC AAGCAGCCCC AGGACCCAGA AGGGTCTGCA TGGGCCATGA GCGGGCACTC
CCAATACAGC TTACCGTACA GGCTTTGGAC ATGCCGGAGG AGGGTGAGGA ACCTGGGGTA
AGCCACAGGG GTGTGGAGGG GCTGTCCCCG CGTCCGCTGA GCCCTGCTCT GCCCCCAGCC
ATCGAGACTT TGCTGTGCTA CCTGGAGCTG CACCCACACC ACTGGCTGGA GCTGCTGGCG
ACCACCTATA CCCATTGCCG TCTGAACTGC CCTGGGGGCC CTGCCCAGCT CCAGGCCCTG
GCCCACAGGT GTCCCCCTTT GGCTGTGTGC TTGGCCCAGC AGCTGCCTGA GGACCCAGGG
CAAGGCAGCA GCTCCGTGGA GTTTGACATG GTCAAGCTGG TGGACTCCAT GGGCTGGGAG
CTGGCCTCTG TGCGGCGGGC TCTCTGCCAG CTGCAGTGGG ACCACGAGCC CAGGACAGGT
GTGCGGCGTG GGACAGGGGT GCTTGTGGAG TTCAGTGAGC TGGCCTTCCA CCTTCGCAGC
CCGGGGGACT TGACCGCTGA GGAGAAGGAC CAGATATGTG ACTTCCTCTA TGGCCGTGTG
CAGGCCCGGG AGCGCCAGGC CCTGGCCCGT CTGCGCAGAA CCTTCCAGGC CTTTCACAGC
GTAGCCTTCC CCAGCTGCGG GCCCTGCCTG GAGCAGCAGG ATGAGGAGCG CAGCACCAGG
CTCAAGGACC TGCTCGGCCG CTACTTTGAG GAAGAGGAAG GGCAGGAGCC GGGAGGCATG
GAGGACGCAC AGGGCCCCGA GCCAGGGCAG GCCAGACTCC AGGATTGGGA GGACCAGGTC
CGCTGCGACA TCCGCCAGTT CCTGTCCCTG AGGCCAGAGG AGAAGTTCTC CAGCAGGGCT
GTGGCCCGCA TCTTCCACGG CATCGGAAGC CCCTGCTACC CGGCCCAGGT GTACGGGCAG
GACCGACGCT TCTGGAGAAA ATACCTGCAC CTGAGCTTCC ATGCCCTGGT GGGCCTGGCC
ACGGAAGAGC TCCTGCAGGT GGCCCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62802
Clone ID Related Accession (Same CDS sequence) XM_011517383
Accession Version XM_011517383.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent DNA helicase Q4 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008046.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGGCGA AGACCTGCGA
GAGCTGCGCA GACATGTGCA CGCCGACAGC ACGGACTTCC TGGCTGTGAA GAGGCTGGTA
CAGCGCGTGT TCCCAGCCTG CACCTGCACC TGCACCAGGC CGCCCTCGGA GCAGGAAGGG
GCCGTGGGTG GGGAGAGGCC TGTGCCCAAG TACCCCCCTC AAGAGGCTGA GCAGCTTAGC
CACCAAGCAG CCCCAGGACC CAGAAGGGTC TGCATGGGCC ATGAGCGGGC ACTCCCAATA
CAGCTTACCG TACAGGCTTT GGACATGCCG GAGGAGGGTG AGGAACCTGG GGTAAGCCAC
AGGGGTGTGG AGGGGCTGTC CCCGCGTCCG CTGAGCCCTG CTCTGCCCCC AGCCATCGAG
ACTTTGCTGT GCTACCTGGA GCTGCACCCA CACCACTGGC TGGAGCTGCT GGCGACCACC
TATACCCATT GCCGTCTGAA CTGCCCTGGG GGCCCTGCCC AGCTCCAGGC CCTGGCCCAC
AGGTGTCCCC CTTTGGCTGT GTGCTTGGCC CAGCAGCTGC CTGAGGACCC AGGGCAAGGC
AGCAGCTCCG TGGAGTTTGA CATGGTCAAG CTGGTGGACT CCATGGGCTG GGAGCTGGCC
TCTGTGCGGC GGGCTCTCTG CCAGCTGCAG TGGGACCACG AGCCCAGGAC AGGTGTGCGG
CGTGGGACAG GGGTGCTTGT GGAGTTCAGT GAGCTGGCCT TCCACCTTCG CAGCCCGGGG
GACTTGACCG CTGAGGAGAA GGACCAGATA TGTGACTTCC TCTATGGCCG TGTGCAGGCC
CGGGAGCGCC AGGCCCTGGC CCGTCTGCGC AGAACCTTCC AGGCCTTTCA CAGCGTAGCC
TTCCCCAGCT GCGGGCCCTG CCTGGAGCAG CAGGATGAGG AGCGCAGCAC CAGGCTCAAG
GACCTGCTCG GCCGCTACTT TGAGGAAGAG GAAGGGCAGG AGCCGGGAGG CATGGAGGAC
GCACAGGGCC CCGAGCCAGG GCAGGCCAGA CTCCAGGATT GGGAGGACCA GGTCCGCTGC
GACATCCGCC AGTTCCTGTC CCTGAGGCCA GAGGAGAAGT TCTCCAGCAG GGCTGTGGCC
CGCATCTTCC ACGGCATCGG AAGCCCCTGC TACCCGGCCC AGGTGTACGG GCAGGACCGA
CGCTTCTGGA GAAAATACCT GCACCTGAGC TTCCATGCCC TGGTGGGCCT GGCCACGGAA
GAGCTCCTGC AGGTGGCCCG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515685.1
CDS38..3541
Misc Feature(1)1454..2005(+)
Misc Feature(2)1526..1966(+)
Misc Feature(3)1550..1564(+)
Misc Feature(4)1850..1861(+)
Translation

Target ORF information:

RefSeq Version XM_011517383
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens RecQ protein-like 4 (RECQL4), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517383

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGGCGA AGACCTGCGA
GAGCTGCGCA GACATGTGCA CGCCGACAGC ACGGACTTCC TGGCTGTGAA GAGGCTGGTA
CAGCGCGTGT TCCCAGCCTG CACCTGCACC TGCACCAGGC CGCCCTCGGA GCAGGAAGGG
GCCGTGGGTG GGGAGAGGCC TGTGCCCAAG TACCCCCCTC AAGAGGCTGA GCAGCTTAGC
CACCAAGCAG CCCCAGGACC CAGAAGGGTC TGCATGGGCC ATGAGCGGGC ACTCCCAATA
CAGCTTACCG TACAGGCTTT GGACATGCCG GAGGAGGGTG AGGAACCTGG GGTAAGCCAC
AGGGGTGTGG AGGGGCTGTC CCCGCGTCCG CTGAGCCCTG CTCTGCCCCC AGCCATCGAG
ACTTTGCTGT GCTACCTGGA GCTGCACCCA CACCACTGGC TGGAGCTGCT GGCGACCACC
TATACCCATT GCCGTCTGAA CTGCCCTGGG GGCCCTGCCC AGCTCCAGGC CCTGGCCCAC
AGGTGTCCCC CTTTGGCTGT GTGCTTGGCC CAGCAGCTGC CTGAGGACCC AGGGCAAGGC
AGCAGCTCCG TGGAGTTTGA CATGGTCAAG CTGGTGGACT CCATGGGCTG GGAGCTGGCC
TCTGTGCGGC GGGCTCTCTG CCAGCTGCAG TGGGACCACG AGCCCAGGAC AGGTGTGCGG
CGTGGGACAG GGGTGCTTGT GGAGTTCAGT GAGCTGGCCT TCCACCTTCG CAGCCCGGGG
GACTTGACCG CTGAGGAGAA GGACCAGATA TGTGACTTCC TCTATGGCCG TGTGCAGGCC
CGGGAGCGCC AGGCCCTGGC CCGTCTGCGC AGAACCTTCC AGGCCTTTCA CAGCGTAGCC
TTCCCCAGCT GCGGGCCCTG CCTGGAGCAG CAGGATGAGG AGCGCAGCAC CAGGCTCAAG
GACCTGCTCG GCCGCTACTT TGAGGAAGAG GAAGGGCAGG AGCCGGGAGG CATGGAGGAC
GCACAGGGCC CCGAGCCAGG GCAGGCCAGA CTCCAGGATT GGGAGGACCA GGTCCGCTGC
GACATCCGCC AGTTCCTGTC CCTGAGGCCA GAGGAGAAGT TCTCCAGCAG GGCTGTGGCC
CGCATCTTCC ACGGCATCGG AAGCCCCTGC TACCCGGCCC AGGTGTACGG GCAGGACCGA
CGCTTCTGGA GAAAATACCT GCACCTGAGC TTCCATGCCC TGGTGGGCCT GGCCACGGAA
GAGCTCCTGC AGGTGGCCCG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62803
Clone ID Related Accession (Same CDS sequence) XM_011517384
Accession Version XM_011517384.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent DNA helicase Q4 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008046.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGGCGA AGACCTGCGA
GAGCTGCGCA GACATGTGCA CGCCGACAGC ACGGACTTCC TGGCTGTGAA GAGGCTGGTA
CAGCGCGTGT TCCCAGCCTG CACCTGCACC TGCACCAGGC CGCCCTCGGA GCAGGAAGGG
GCCGTGGGTG GGGAGAGGCC TGTGCCCAAG TACCCCCCTC AAGAGGCTGA GCAGCTTAGC
CACCAAGCAG CCCCAGGACC CAGAAGGGTC TGCATGGGCC ATGAGCGGGC ACTCCCAATA
CAGCTTACCG TACAGGCTTT GGACATGCCG GAGGAGGCCA TCGAGACTTT GCTGTGCTAC
CTGGAGCTGC ACCCACACCA CTGGCTGGAG CTGCTGGCGA CCACCTATAC CCATTGCCGT
CTGAACTGCC CTGGGGGCCC TGCCCAGCTC CAGGCCCTGG CCCACAGGTG TCCCCCTTTG
GCTGTGTGCT TGGCCCAGCA GCTGCCTGAG GACCCAGGGC AAGGCAGCAG CTCCGTGGAG
TTTGACATGG TCAAGCTGGT GGACTCCATG GGCTGGGAGC TGGCCTCTGT GCGGCGGGCT
CTCTGCCAGC TGCAGTGGGA CCACGAGCCC AGGACAGGTG TGCGGCGTGG GACAGGGGTG
CTTGTGGAGT TCAGTGAGCT GGCCTTCCAC CTTCGCAGCC CGGGGGACTT GACCGCTGAG
GAGAAGGACC AGATATGTGA CTTCCTCTAT GGCCGTGTGC AGGCCCGGGA GCGCCAGGCC
CTGGCCCGTC TGCGCAGAAC CTTCCAGGCC TTTCACAGCG TAGCCTTCCC CAGCTGCGGG
CCCTGCCTGG AGCAGCAGGA TGAGGAGCGC AGCACCAGGC TCAAGGACCT GCTCGGCCGC
TACTTTGAGG AAGAGGAAGG GCAGGAGCCG GGAGGCATGG AGGACGCACA GGGCCCCGAG
CCAGGGCAGG CCAGACTCCA GGATTGGGAG GACCAGGTCC GCTGCGACAT CCGCCAGTTC
CTGTCCCTGA GGCCAGAGGA GAAGTTCTCC AGCAGGGCTG TGGCCCGCAT CTTCCACGGC
ATCGGAAGCC CCTGCTACCC GGCCCAGGTG TACGGGCAGG ACCGACGCTT CTGGAGAAAA
TACCTGCACC TGAGCTTCCA TGCCCTGGTG GGCCTGGCCA CGGAAGAGCT CCTGCAGGTG
GCCCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515686.1
CDS38..3466
Misc Feature(1)1454..2005(+)
Misc Feature(2)1526..1966(+)
Misc Feature(3)1550..1564(+)
Misc Feature(4)1850..1861(+)
Translation

Target ORF information:

RefSeq Version XM_011517384
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens RecQ protein-like 4 (RECQL4), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517384

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGGCGA AGACCTGCGA
GAGCTGCGCA GACATGTGCA CGCCGACAGC ACGGACTTCC TGGCTGTGAA GAGGCTGGTA
CAGCGCGTGT TCCCAGCCTG CACCTGCACC TGCACCAGGC CGCCCTCGGA GCAGGAAGGG
GCCGTGGGTG GGGAGAGGCC TGTGCCCAAG TACCCCCCTC AAGAGGCTGA GCAGCTTAGC
CACCAAGCAG CCCCAGGACC CAGAAGGGTC TGCATGGGCC ATGAGCGGGC ACTCCCAATA
CAGCTTACCG TACAGGCTTT GGACATGCCG GAGGAGGCCA TCGAGACTTT GCTGTGCTAC
CTGGAGCTGC ACCCACACCA CTGGCTGGAG CTGCTGGCGA CCACCTATAC CCATTGCCGT
CTGAACTGCC CTGGGGGCCC TGCCCAGCTC CAGGCCCTGG CCCACAGGTG TCCCCCTTTG
GCTGTGTGCT TGGCCCAGCA GCTGCCTGAG GACCCAGGGC AAGGCAGCAG CTCCGTGGAG
TTTGACATGG TCAAGCTGGT GGACTCCATG GGCTGGGAGC TGGCCTCTGT GCGGCGGGCT
CTCTGCCAGC TGCAGTGGGA CCACGAGCCC AGGACAGGTG TGCGGCGTGG GACAGGGGTG
CTTGTGGAGT TCAGTGAGCT GGCCTTCCAC CTTCGCAGCC CGGGGGACTT GACCGCTGAG
GAGAAGGACC AGATATGTGA CTTCCTCTAT GGCCGTGTGC AGGCCCGGGA GCGCCAGGCC
CTGGCCCGTC TGCGCAGAAC CTTCCAGGCC TTTCACAGCG TAGCCTTCCC CAGCTGCGGG
CCCTGCCTGG AGCAGCAGGA TGAGGAGCGC AGCACCAGGC TCAAGGACCT GCTCGGCCGC
TACTTTGAGG AAGAGGAAGG GCAGGAGCCG GGAGGCATGG AGGACGCACA GGGCCCCGAG
CCAGGGCAGG CCAGACTCCA GGATTGGGAG GACCAGGTCC GCTGCGACAT CCGCCAGTTC
CTGTCCCTGA GGCCAGAGGA GAAGTTCTCC AGCAGGGCTG TGGCCCGCAT CTTCCACGGC
ATCGGAAGCC CCTGCTACCC GGCCCAGGTG TACGGGCAGG ACCGACGCTT CTGGAGAAAA
TACCTGCACC TGAGCTTCCA TGCCCTGGTG GGCCTGGCCA CGGAAGAGCT CCTGCAGGTG
GCCCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62804
Clone ID Related Accession (Same CDS sequence) XM_011517385
Accession Version XM_011517385.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ATP-dependent DNA helicase Q4 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008046.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGGAAGCAG AAGTGGCGGA AGAAAGGGGA GTGTTTTGGG GGTGGTGGTG CCACAGTCAC 
AACCAAGGAG TCTTGTTTCC TGAACGAGCA GTTCGATCAC TGGGCAGCCC AGTGTCCCCG
GCCAGTGAGG AAGACACAGA TGCTGTTGGG CCTGAGCCAC TGGTTCCTTC ACCACAACCT
GTACCTGAGG TGCCCAGCCT GGACCCCACC GTGCTGCCAC TCTACTCCCT GGGGCCCTCA
GGGCAGTTGG CAGAGACGCC GGCTGAGGTG TTCCAGGCCC TGGAGCAGCT GGGGCACCAA
GCCTTTCGCC CTGGGCAGGA GCGTGCAGTC ATGCGGATCC TGTCTGGCAT CTCCACGCTG
CTGGTGCTGC CTACAGGTGC CGGCAAGTCC CTGTGCTACC AGCTCCCAGC GCTGCTCTAC
AGCCGGCGCA GCCCCTGCCT CACGTTGGTC GTCTCTCCCC TGCTGTCACT CATGGATGAC
CAGGTGTCTG GCCTGCCACC GTGTCTCAAG GCGGCCTGCA TACACTCGGG CATGACCAGG
AAGCAACGGG AATCTGTCCT GCAGAAGATT CGGGCAGCCC AGGTACACGT GCTGATGCTG
ACACCTGAGG CACTGGTGGG GGCGGGAGGC CTCCCTCCAG CCGCACAGCT GCCTCCAGTT
GCTTTTGCCT GCATTGATGA GGCCCACTGC CTCTCCCAGT GGTCCCACAA CTTCCGGCCC
TGCTACCTGC GCGTCTGCAA GGTGCTTCGG GAGCGCATGG GCGTGCACTG CTTCCTGGGC
CTCACAGCCA CAGCCACACG CCGCACTGCC AGTGACGTGG CACAGCACCT GGCTGTGGCT
GAAGAGCCTG ACCTCCACGG GCCAGCCCCA GTTCCCACCA ACCTGCACCT TTCCGTGTCC
ATGGACAGGG ACACAGACCA GGCACTGTTG ACGCTGCTGC AAGGCAAACG TTTTCAAAAC
CTCGATTCCA TTATCATTTA CTGCAACCGG CGCGAGGACA CAGAGCGGAT CGCTGCGCTC
CTCCGAACCT GCCTGCACGC AGCCTGGGTC CCAGGGTCTG GAGGTCGTGC CCCCAAAACC
ACAGCCGAGG CCTACCACGC GGGCATGTGC AGCCGGGAAC GGCGGCGGGT ACAGCGAGCC
TTCATGCAGG GCCAGTTGCG GGTGGTGGTG GCCACGGTGG CCTTTGGGAT GGGGCTGGAC
CGGCCAGATG TGCGGGCTGT GCTGCATCTG GGGCTGCCCC CAAGCTTCGA GAGCTACGTG
CAGGCCGTGG GCCGGGCCGG GCGTGACGGG CAGCCTGCCC ACTGCCACCT CTTCCTGCAG
CCCCAGGGCG AAGACCTGCG AGAGCTGCGC AGACATGTGC ACGCCGACAG CACGGACTTC
CTGGCTGTGA AGAGGCTGGT ACAGCGCGTG TTCCCAGCCT GCACCTGCAC CTGCACCAGG
CCGCCCTCGG AGCAGGAAGG GGCCGTGGGT GGGGAGAGGC CTGTGCCCAA GTACCCCCCT
CAAGAGGCTG AGCAGCTTAG CCACCAAGCA GCCCCAGGAC CCAGAAGGGT CTGCATGGGC
CATGAGCGGG CACTCCCAAT ACAGCTTACC GTACAGGCTT TGGACATGCC GGAGGAGGGT
GAGGAACCTG GGGTAAGCCA CAGGGGTGTG GAGGGGCTGT CCCCGCGTCC GCTGAGCCCT
GCTCTGCCCC CAGCCATCGA GACTTTGCTG TGCTACCTGG AGCTGCACCC ACACCACTGG
CTGGAGCTGC TGGCGACCAC CTATACCCAT TGCCGTCTGA ACTGCCCTGG GGGCCCTGCC
CAGCTCCAGG CCCTGGCCCA CAGGTGTCCC CCTTTGGCTG TGTGCTTGGC CCAGCAGCTG
CCTGAGGACC CAGGGCAAGG CAGCAGCTCC GTGGAGTTTG ACATGGTCAA GCTGGTGGAC
TCCATGGGCT GGGAGCTGGC CTCTGTGCGG CGGGCTCTCT GCCAGCTGCA GTGGGACCAC
GAGCCCAGGA CAGGTGTGCG GCGTGGGACA GGGGTGCTTG TGGAGTTCAG TGAGCTGGCC
TTCCACCTTC GCAGCCCGGG GGACTTGACC GCTGAGGAGA AGGACCAGAT ATGTGACTTC
CTCTATGGCC GTGTGCAGGC CCGGGAGCGC CAGGCCCTGG CCCGTCTGCG CAGAACCTTC
CAGGCCTTTC ACAGCGTAGC CTTCCCCAGC TGCGGGCCCT GCCTGGAGCA GCAGGATGAG
GAGCGCAGCA CCAGGCTCAA GGACCTGCTC GGCCGCTACT TTGAGGAAGA GGAAGGGCAG
GAGCCGGGAG GCATGGAGGA CGCACAGGGC CCCGAGCCAG GGCAGGCCAG ACTCCAGGAT
TGGGAGGACC AGGTCCGCTG CGACATCCGC CAGTTCCTGT CCCTGAGGCC AGAGGAGAAG
TTCTCCAGCA GGGCTGTGGC CCGCATCTTC CACGGCATCG GAAGCCCCTG CTACCCGGCC
CAGGTGTACG GGCAGGACCG ACGCTTCTGG AGAAAATACC TGCACCTGAG CTTCCATGCC
CTGGTGGGCC TGGCCACGGA AGAGCTCCTG CAGGTGGCCC GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515687.1
CDS96..2660
Misc Feature(1)375..926(+)
Misc Feature(2)447..887(+)
Misc Feature(3)471..485(+)
Misc Feature(4)771..782(+)
Misc Feature(5)1011..1409(+)
Misc Feature(6)1080..1277(+)
Misc Feature(7)1293..1379(+)
Translation

Target ORF information:

RefSeq Version XM_011517385
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens RecQ protein-like 4 (RECQL4), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517385

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGGAAGCAG AAGTGGCGGA AGAAAGGGGA GTGTTTTGGG GGTGGTGGTG CCACAGTCAC 
AACCAAGGAG TCTTGTTTCC TGAACGAGCA GTTCGATCAC TGGGCAGCCC AGTGTCCCCG
GCCAGTGAGG AAGACACAGA TGCTGTTGGG CCTGAGCCAC TGGTTCCTTC ACCACAACCT
GTACCTGAGG TGCCCAGCCT GGACCCCACC GTGCTGCCAC TCTACTCCCT GGGGCCCTCA
GGGCAGTTGG CAGAGACGCC GGCTGAGGTG TTCCAGGCCC TGGAGCAGCT GGGGCACCAA
GCCTTTCGCC CTGGGCAGGA GCGTGCAGTC ATGCGGATCC TGTCTGGCAT CTCCACGCTG
CTGGTGCTGC CTACAGGTGC CGGCAAGTCC CTGTGCTACC AGCTCCCAGC GCTGCTCTAC
AGCCGGCGCA GCCCCTGCCT CACGTTGGTC GTCTCTCCCC TGCTGTCACT CATGGATGAC
CAGGTGTCTG GCCTGCCACC GTGTCTCAAG GCGGCCTGCA TACACTCGGG CATGACCAGG
AAGCAACGGG AATCTGTCCT GCAGAAGATT CGGGCAGCCC AGGTACACGT GCTGATGCTG
ACACCTGAGG CACTGGTGGG GGCGGGAGGC CTCCCTCCAG CCGCACAGCT GCCTCCAGTT
GCTTTTGCCT GCATTGATGA GGCCCACTGC CTCTCCCAGT GGTCCCACAA CTTCCGGCCC
TGCTACCTGC GCGTCTGCAA GGTGCTTCGG GAGCGCATGG GCGTGCACTG CTTCCTGGGC
CTCACAGCCA CAGCCACACG CCGCACTGCC AGTGACGTGG CACAGCACCT GGCTGTGGCT
GAAGAGCCTG ACCTCCACGG GCCAGCCCCA GTTCCCACCA ACCTGCACCT TTCCGTGTCC
ATGGACAGGG ACACAGACCA GGCACTGTTG ACGCTGCTGC AAGGCAAACG TTTTCAAAAC
CTCGATTCCA TTATCATTTA CTGCAACCGG CGCGAGGACA CAGAGCGGAT CGCTGCGCTC
CTCCGAACCT GCCTGCACGC AGCCTGGGTC CCAGGGTCTG GAGGTCGTGC CCCCAAAACC
ACAGCCGAGG CCTACCACGC GGGCATGTGC AGCCGGGAAC GGCGGCGGGT ACAGCGAGCC
TTCATGCAGG GCCAGTTGCG GGTGGTGGTG GCCACGGTGG CCTTTGGGAT GGGGCTGGAC
CGGCCAGATG TGCGGGCTGT GCTGCATCTG GGGCTGCCCC CAAGCTTCGA GAGCTACGTG
CAGGCCGTGG GCCGGGCCGG GCGTGACGGG CAGCCTGCCC ACTGCCACCT CTTCCTGCAG
CCCCAGGGCG AAGACCTGCG AGAGCTGCGC AGACATGTGC ACGCCGACAG CACGGACTTC
CTGGCTGTGA AGAGGCTGGT ACAGCGCGTG TTCCCAGCCT GCACCTGCAC CTGCACCAGG
CCGCCCTCGG AGCAGGAAGG GGCCGTGGGT GGGGAGAGGC CTGTGCCCAA GTACCCCCCT
CAAGAGGCTG AGCAGCTTAG CCACCAAGCA GCCCCAGGAC CCAGAAGGGT CTGCATGGGC
CATGAGCGGG CACTCCCAAT ACAGCTTACC GTACAGGCTT TGGACATGCC GGAGGAGGGT
GAGGAACCTG GGGTAAGCCA CAGGGGTGTG GAGGGGCTGT CCCCGCGTCC GCTGAGCCCT
GCTCTGCCCC CAGCCATCGA GACTTTGCTG TGCTACCTGG AGCTGCACCC ACACCACTGG
CTGGAGCTGC TGGCGACCAC CTATACCCAT TGCCGTCTGA ACTGCCCTGG GGGCCCTGCC
CAGCTCCAGG CCCTGGCCCA CAGGTGTCCC CCTTTGGCTG TGTGCTTGGC CCAGCAGCTG
CCTGAGGACC CAGGGCAAGG CAGCAGCTCC GTGGAGTTTG ACATGGTCAA GCTGGTGGAC
TCCATGGGCT GGGAGCTGGC CTCTGTGCGG CGGGCTCTCT GCCAGCTGCA GTGGGACCAC
GAGCCCAGGA CAGGTGTGCG GCGTGGGACA GGGGTGCTTG TGGAGTTCAG TGAGCTGGCC
TTCCACCTTC GCAGCCCGGG GGACTTGACC GCTGAGGAGA AGGACCAGAT ATGTGACTTC
CTCTATGGCC GTGTGCAGGC CCGGGAGCGC CAGGCCCTGG CCCGTCTGCG CAGAACCTTC
CAGGCCTTTC ACAGCGTAGC CTTCCCCAGC TGCGGGCCCT GCCTGGAGCA GCAGGATGAG
GAGCGCAGCA CCAGGCTCAA GGACCTGCTC GGCCGCTACT TTGAGGAAGA GGAAGGGCAG
GAGCCGGGAG GCATGGAGGA CGCACAGGGC CCCGAGCCAG GGCAGGCCAG ACTCCAGGAT
TGGGAGGACC AGGTCCGCTG CGACATCCGC CAGTTCCTGT CCCTGAGGCC AGAGGAGAAG
TTCTCCAGCA GGGCTGTGGC CCGCATCTTC CACGGCATCG GAAGCCCCTG CTACCCGGCC
CAGGTGTACG GGCAGGACCG ACGCTTCTGG AGAAAATACC TGCACCTGAG CTTCCATGCC
CTGGTGGGCC TGGCCACGGA AGAGCTCCTG CAGGTGGCCC GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The intrinsically disordered amino-terminal region of human RecQL4: multiple DNA-binding domains confer annealing, strand exchange and G4 DNA binding
Nucleic Acids Res. 42 (20), 12614-12627 (2014)
Keller H, Kiosze K, Sachsenweger J, Haumann S, Ohlenschlager O, Nuutinen T, Syvaoja JE, Gorlach M, Grosse F and Pospiech H.


book

RECQ helicase RECQL4 participates in non-homologous end joining and interacts with the Ku complex
Carcinogenesis 35 (11), 2415-2424 (2014)
Shamanna RA, Singh DK, Lu H, Mirey G, Keijzers G, Salles B, Croteau DL and Bohr VA.


book

Common genetic variants associated with cognitive performance identified using the proxy-phenotype method
Proc. Natl. Acad. Sci. U.S.A. 111 (38), 13790-13794 (2014)
Rietveld CA, Esko T, Davies G, Pers TH, Turley P, Benyamin B, Chabris CF, Emilsson V, Johnson AD, Lee JJ, de Leeuw C, Marioni RE, Medland SE, Miller MB, Rostapshova O, van der Lee SJ, Vinkhuyzen AA, Amin N, Conley D, Derringer J, van Duijn CM, Fehrmann R, Franke L, Glaeser EL, Hansell NK, Hayward C, Iacono WG, Ibrahim-Verbaas C, Jaddoe V, Karjalainen J, Laibson D, Lichtenstein P, Liewald DC, Magnusson PK, Martin NG, McGue M, McMahon G, Pedersen NL, Pinker S, Porteous DJ, Posthuma D, Rivadeneira F, Smith BH, Starr JM, Tiemeier H, Timpson NJ, Trzaskowski M, Uitterlinden AG, Verhulst FC, Ward ME, Wright MJ, Davey Smith G, Deary IJ, Johannesson M, Plomin R, Visscher PM, Benjamin DJ, Cesarini D and Koellinger PD.


book

RECQ DNA helicases and osteosarcoma
Adv. Exp. Med. Biol. 804, 129-145 (2014)
Lu L, Jin W, Liu H and Wang LL.


book

Rothmund-thomson syndrome responsible gene, RECQL4: genomic structure and products
Genomics 61 (3), 268-276 (1999)
Kitao S, Lindor NM, Shiratori M, Furuichi Y and Shimamoto A.


book

Mutations in RECQL4 cause a subset of cases of Rothmund-Thomson syndrome
Nat. Genet. 22 (1), 82-84 (1999)
Kitao S, Shimamoto A, Goto M, Miller RW, Smithson WA, Lindor NM and Furuichi Y.


book

Cloning of two new human helicase genes of the RecQ family: biological significance of multiple species in higher eukaryotes
Genomics 54 (3), 443-452 (1998)
Kitao S, Ohsugi I, Ichikawa K, Goto M, Furuichi Y and Shimamoto A.


book

Rothmund-Thomson Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Wang,L.L. and Plon,S.E.


book

Baller-Gerold Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Van Maldergem,L.


book

Intrafusal fibre types in rat limb muscle spindles: morphological and histochemical characteristics
Histochemistry 47 (1), 43-57 (1976)
Soukup,T.


 
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